Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,359,250	A→C	15.8%	S261R (AGT→AGG)	cadB ←	putative lysine/cadaverine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,359,250	0	A	C	15.8%	183.3 / 4.5	87	S261R (AGT→AGG)	cadB	putative lysine/cadaverine transporter
Reads supporting (aligned to +/- strand):  ref base A (39/30);  new base C (4/12);  total (43/44)
Fisher's exact test for biased strand distribution p-value = 2.83e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCATCAGAGTGATAAGGATCATCAGGGCAGTCATTTTCACTGCAGCCAGCAGCAGACCTTTTTT‑CGG‑AATACCGTTGCTGTCGACTTCACCAT‑AAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGT  >  NC_000913/4359012‑4359486                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             tatgcaggtgtaTAAGGTTCAGCAGGGCAGTCATTTTCCCTGACGCCAGCAGCAGACCTTTTTT‑CGG‑AATTCCGTTGCTGCCGCCTTCACCAT‑AAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCa                                                                                                                                                                                                                                              <  2:270490/228‑1 (MQ=255)  catcaGAGTGATAAGGGTCTTCAGGGCAGTCATTTTCACTGCAGCCAGCAGCAGACCTTTTTG‑CGG‑AATACCGTTGCTGTCGACTTCACCAT‑AAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCAc                                                                                                                                                                                                                                             <  2:747806/239‑1 (MQ=255)   aacatagTGTTAAGGTTCCTCAGGGCGTTCATTTTCACTGCGGCTAGCAGCAGACCTTTTTTCCGG‑AATACCGTTGCTGTCGACTTCACCAT‑AAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTCCACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCAc                                                                                                                                                                                                                                             <  2:255556/234‑1 (MQ=255)        agTGATAAGGATCATCAGGGCAGTCATTTTCACTGCAGCCAGCAGCAGACCTTTTTT‑CGG‑AATACCGTTGCTGTCGACTTCACCAT‑AAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTg                                                                                                                                                                                                                                       >  1:383470/1‑239 (MQ=255)          tGATAAGGATCATCAGGGCAGTCATTTTCACTGCAGCCAGCAGCAGACCTTTTTT‑CGG‑AATACCGTTGCTGTCGACTTCACCAT‑AAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGACCTGATTGCa                                                                                                                                                                                                                                     >  1:105532/1‑239 (MQ=255)          tGATAAGGATCATCAGGGCAGTCATTTTCACTGCAGCCAGCAGCAGACCTTTTTT‑CGG‑AATACCGTTGCTGTCGACTTCACCAT‑AAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGGG‑AATGCAGAAACCAGCGGCCCA‑GCCCAGGTACC‑CAGGATAGTTGAAGACCGGATTGCa                                                                                                                                                                                                                                     >  2:95665/1‑239 (MQ=255)          tGATAAGGATCATCAGGGCAGTCATTTACACTGCAGGCAGCAGCAGGCCTTGTTT‑CGG‑AATGCCGTTGGTGTCGACTTCACCAT‑AATCTTGCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGc                                                                                                                                                                                                                                      <  1:376859/238‑1 (MQ=255)                  atcatcAGGGCAGTCATTTTCACTGCAGCCAGCAGCAGACCTTTTTT‑CGG‑AATACCGTTGCTGTCGACTTCACCAT‑AAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCCGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑ACCCCATTAAC‑GGGGAAAGTTGAAAACCAGATGGCAAACgggga                                                                                                                                                                                                                             >  2:117438/1‑236 (MQ=255)                  atcatcAGGGCAGTCATTTTCACTGCAGCCAGCAGCAGACCTTTTTT‑CGG‑AATACCGTTGCTGTCGACTTCACCAT‑AAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑ATTGAAGAAACCAGCGCCGCA‑GCCCAGTTACC‑GAGGCTAGTTGAAGAACTGATTGCAAACGGAg                                                                                                                                                                                                                              >  1:353003/1‑238 (MQ=255)                    catcaGGGCAGTCATTTTCACTGCAGCCAGCAGCAGACCTTTTTT‑CGG‑AATACCGTTGCTGTCGACTTCACCAT‑AAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCCGTTACC‑GAGGATAGTTTAAGCACTCATTGCACACGGAGCa                                                                                                                                                                                                                            >  1:846494/1‑238 (MQ=255)                    catcaGGGCAGTCATTTTCACTGCAGCCAGCAGCAGACCTTTTTT‑CGG‑AATACCGTTGCTGTCGACTTCACCAT‑AAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGAACTGATTGCAAACGGTGCa                                                                                                                                                                                                                            >  1:201905/1‑238 (MQ=255)                      tcaGGGCAGTCATTTTCACTGCAGCCAGCAGCAGACCTTTTTT‑CGG‑AATACCGTTGCTGTCGACTTCACCAT‑AAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCg                                                                                                                                                                                                                         >  1:284827/1‑239 (MQ=255)                          ggCAGTCATTTTCACTGCAGCCAGCAGCAGACCTTTTTT‑CGG‑AATACCGTTGCTGTCGACTTCACCAT‑AAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCCCGCAAAGGCGGTG‑AATGCAAAAACCAGAGGCGAA‑GCCCCATTACC‑GAGGAAAGTTGAAACCCGAGTTGCAAAAGGGGCACCGGaa                                                                                                                                                                                                                      >  2:280503/1‑238 (MQ=255)                           gCAGTCATTTTCACTGCAGCCAGCAGCAGACCTTTTTT‑CGG‑AATACCGTTGCTGTCGACTTCACCAT‑AAACTTTCGGGAAGTTACCGTCGTTAGCGGCACTTACCCCTGCCTGGCCTACCAACATAATCCAGGAGCCCAGAGAAGCCAGGAACTCAAAGACGGGG‑AATGCAGAAACCACCGG‑GCACGCCAAGTAACC‑GAGCAAAGGTGAAGCACGAATTACaaaggcgaaaccggaaaa                                                                                                                                                                                                                    >  2:254579/1‑224 (MQ=255)                            gtttttttttCCCTGCCCCCCGCCGCACACTTTTTTT‑GGG‑ACTCCGGTTGCTGTCCACTTC‑CCATAAAACTGACGGGAAGTTACCGTTGTTAGCGGCACGACCCCCTTCCTGGCCTACCAACATCACCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAACCCAGCGGCGCA‑GCCCAGTTCCC‑GAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAgc                                                                                                                                                                                                                    <  2:304077/232‑1 (MQ=255)                            cAGTCATTTTCCCGGCAGCCAGCAGCAGACCTTTTTG‑CGG‑AATCCCGTTGCTGTCGCCTGCACCAT‑AAACTTGCGGGAAGTTACCGGCGTTAGCGGCACGTACCCCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAgc                                                                                                                                                                                                                    <  1:322416/238‑1 (MQ=255)                             aGTCATTTTCCCTGCAGCCAGCAGCAGACCTTTTTT‑CGG‑AATCCCGTTGCTGTCGACTTCACCAT‑AAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAgcc                                                                                                                                                                                                                   <  1:221018/238‑1 (MQ=255)                              gtttttttCCCTTCCGCCCGCAGCAGTCTTTTTTT‑GTG‑AATCCCGTTGCGGTCGATTTCACCAA‑AACCTTTCGGGAAGTTCCCGTCGTTAGCGGCACGTACCCCGGCCTGGCCTACAACCATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AAGGCAGAACCCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCCCTGATTGCAAACGGAGCACCGGAAgccgc                                                                                                                                                                                                                 <  2:24001/235‑1 (MQ=255)                              gTCATTTTCCCTGCAGCCAGCAGCAGCCCTTTTTT‑CGG‑AATACCGTTGCTGTCGACTTCACCAT‑AAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTCCACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCCCTGATTGCAAACGGAGCCCCGGAAgccgc                                                                                                                                                                                                                 <  1:902869/239‑1 (MQ=255)                               tCATTTTCACTGCAGCCAGCAGCAGACCTTTTTT‑CGG‑AATACCGTTGCTGTCGACTTCACCAT‑AAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGAGAGTTGAAGCACTGATTGCAAAAGGAGAAACGGAAaccgca                                                                                                                                                                                                                >  2:782006/1‑238 (MQ=255)                                   tttCCCGGCACCCAGCAGCAGCCCTTTTTT‑GGG‑AATCCCGTTGGTGTCGACTTCACCAT‑AAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCGGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTa                                                                                                                                                                                                            <  1:95665/239‑1 (MQ=255)                                         tGCAGCCAGCAGCAGACATTTTTT‑CGG‑AATACCGTTGCTGTCGACTTCCCCAT‑AAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCCGAGAAGTCAGGCACGCAAAGTAGGTG‑AATGGAGAAACCAGCGGCTCG‑GCCCAGTAACC‑GAGAATAGGTGAGAAACGAAATGAAAAAGGAGCAACGGAAGCGGCAAATAAagaaa                                                                                                                                                                                                      >  2:458807/1‑238 (MQ=255)                                          gcacccacctccaccccTTTTTT‑CGG‑AATCCCGTTGCTGTCGACTCCACCAT‑AAGCTTGCGGGAATTTCCCGGCGTTAGCGGCACGTACACCGGCCTGGCCTACAAACATCACCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGCAACCGGAGCACCGGAAGCCGCCATTACagaaga                                                                                                                                                                                                     <  2:616074/224‑1 (MQ=255)                                                cacccccctccTTTTTT‑CGGAAATCCCTTTGCTTTCGCTTTCCCAAT‑AAACTTCGGGAAATTTACCGTCGTTAGCGGCACGTCCCCCTGCCTGGCCTACCAACATAAACCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAAAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGAAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGat                                                                                                                                                                                                <  2:383470/230‑1 (MQ=255)                                                agcagcagACCTTTTTT‑CGG‑AATACCGTTGCTGTCGACTTCACCAT‑AAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGGGCACCGGAAGCCGCCATTAAAAAAGCAGGata                                                                                                                                                                                               >  2:885269/1‑239 (MQ=255)                                                                      aaTACCGTTGCTGTCGACTTCACCAT‑AAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGCTAGTTGAATCACTGATTGCAAACGGAGCACCGGAAACACCCATTCAAGAAGACGGCGACAAACCgagaaaacccaagg                                                                                                                                                                           >  2:853604/1‑226 (MQ=255)                                                                                cTGTCGGCTTCACCAT‑AAACTTTCGGGAAGTTCCCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAAGCACGCGACGGCGGTG‑AATGCAGAAACCAGCGGCCAA‑GACCAGGAAAC‑TAGGAGAGTTGAAGCACTACTTTCAAACGACCAACTGGAATCCGCCATTTAGTGAGACGGCTACAAACCAGGAAACACCTGAGGCGCAGCGAt                                                                                                                                                                 >  2:899489/1‑239 (MQ=255)                                                                                   tCGACTTCACCAT‑AAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGAACTGATTGCCAACGGAGCACCGGAAGCCGCCATTACAGCAGAAGGAACAATACCGGAAAGCACCTGAGCCCCAACGATgaa                                                                                                                                                              >  2:177551/1‑237 (MQ=255)                                                                                                aaaccTTGCGGGAATGTACCGTTCTTAGCGGCACGTCACCCTGCCTGGCCTACCAACACCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCCCTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGc                                                                                                                                                  <  1:209640/235‑1 (MQ=255)                                                                                                  accTTCCGGGAATTTACCGTCGTTAGCGGCACGGCCCCCGGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCAAGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTa                                                                                                                                                <  1:714131/237‑1 (MQ=255)                                                                                                        cGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCACAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAACCACTGCTTGCAAACGGAGCACCGGAAGCCGCCATTACAGAATACGGATACATACCGGAAAGCACCTGAGCCGCAGCGATGTAACCAATACCTACTAAACCAg                                                                                                                                          >  1:676002/1‑239 (MQ=255)                                                                                                                 cccctctttAGCGGCCCGTCCCCCTGCCTGGCCTACCAACAACATCCAGGACCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAACCCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGAAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCa                                                                                                                                 <  2:284827/232‑1 (MQ=255)                                                                                                                       gtTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGACGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCCTTACAGAAGACGGATACATACCGGAAAGAACCCCAGCCCCCCGCATGTAAACAAAACCCCCAAAACCaaacccccaaacgact                                                                                                                            >  2:367390/1‑223 (MQ=255)                                                                                                                                ctgtccccctgccggGCCACCCAACATCACCCAGGGCCACAGGGAAGT‑AGGGCCGGAAAAGGGGTGAAATGCAGAAACCAGGGGAGCA‑GCCCATTTTCC‑GAGGATAGTGGAAGCCCGGTTGGCAAACGGGGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGa                                                                                                                   <  2:656449/231‑1 (MQ=255)                                                                                                                                 cGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGacc                                                                                                                 >  1:165193/1‑237 (MQ=255)                                                                                                                                         tgcctgGCATCCCAACAACACCCAGGAGCCAAGAAAAGTCGGGCACGAAAGGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCGGTTACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGtt                                                                                                         <  1:864368/239‑1 (MQ=255)                                                                                                                                          gcctgGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGCTTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCCGAATCCCAGCGATGTAAACAATACCCGCTAAACCAAAACCCACCAAGGTAGCCAGCGGAACGGTaaattt                                                                                                        >  2:213673/1‑234 (MQ=255)                                                                                                                                           cctgGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTc                                                                                                       >  1:777815/1‑239 (MQ=255)                                                                                                                                            ctgGCCTACCAACATCATCCAGGGCCCCAGAGAAGCCAGGCACGCAAAGGCGGTG‑AAGGCAAAAACCAGCGGCGAA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTc                                                                                                       <  1:487741/238‑1 (MQ=255)                                                                                                                                            ctgGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGAt                                                                                                                                                                 <  1:482477/180‑1 (MQ=255)                                                                                                                                            ctgGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGAt                                                                                                                                                                 >  2:482477/1‑180 (MQ=255)                                                                                                                                            ctgGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAAACGGATACATACCGGAAAGGACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGAACCCACCAAGGCTGCCAGGCGAACGGGACCttcgg                                                                                                      >  2:180106/1‑236 (MQ=255)                                                                                                                                             tgGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAAc                                                                                                                                             >  1:619927/1‑199 (MQ=255)                                                                                                                                                 cTACCAACCACCACCAGGATGCGAGAGAAGCCAGGCACGCAAGGGCGGTG‑AATGCAGAACCCAGCGGCCCA‑GCCCAGTAACC‑GAGGATAGTTGAAGCCCGGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCCCCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGttt                                                                                                 <  1:108007/239‑1 (MQ=255)                                                                                                                                                  tACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGgttt                                                                                                >  1:80895/1‑239 (MQ=255)                                                                                                                                                        catcctCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCa                                                                                          <  1:885269/239‑1 (MQ=255)                                                                                                                                                          tcatcCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTGGCCAGCGGAACGGGAAGTTTCGGGTTTTTAACCAt                                                                                         >  2:749216/1‑238 (MQ=255)                                                                                                                                                             tcCAGGAGCCCAGAGAAGTCAGGCACGCAAGGGCGGTG‑AATGCAGAACCCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCa                                                                                     <  1:280503/239‑1 (MQ=255)                                                                                                                                                             tcCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTg                                                                                                                                                                                                                                           <  2:657537/89‑1 (MQ=255)                                                                                                                                                             tcCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTg                                                                                                                                                                                                                                           >  1:657537/1‑89 (MQ=255)                                                                                                                                                              ccAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGAATACATACCGGAAAGCACCTGAGTCGCAGCGATGTACACAATACCTGCTAAACCAGTACCCAGCATGGGTGCCAGCGGCACGGGACAGTTACGGTTTTTAACCaaaacac                                                                                    >  2:131950/1‑233 (MQ=255)                                                                                                                                                                   aGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAACCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGAACCCAGAAAGGTTGCCACAGGAACGGAACGTTTCGGGTTTTTAAcaaaacaacaaata                                                                               >  2:20622/1‑226 (MQ=255)                                                                                                                                                                       cAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACagaa                                                                                                                                                                                                       >  2:84404/1‑115 (MQ=255)                                                                                                                                                                       cAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACagaa                                                                                                                                                                                                       <  1:84404/115‑1 (MQ=255)                                                                                                                                                                           gaAGTCAGGCCGGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCCCTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTg                                                                        <  1:210446/238‑1 (MQ=255)                                                                                                                                                                            aaGTCAGGCACGCAAGGGCGGTG‑AATGCAAAAACCAGCAGCGCG‑CCCCGGTTACG‑GTGGGTGTTGGAACCCCTGAGTGAAACGGGAGGCCCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCGGAGTCGCAGCGATGTAAACAATCCCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGc                                                                       <  2:29066/238‑1 (MQ=255)                                                                                                                                                                               tCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCgggt                                                                   >  1:292271/1‑237 (MQ=255)                                                                                                                                                                                  ggCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGAGGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGGTTCGGGGTTTTAACCATACCAGAACTTACAGCCGAGGAttaa                                                                >  2:222904/1‑237 (MQ=255)                                                                                                                                                                                      cGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAaca                                                             >  2:580887/1‑238 (MQ=255)                                                                                                                                                                                        cAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGAACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCAAACAAGTACTTAAAGCTGCGGATTAAaaacac                                                          >  2:877382/1‑237 (MQ=255)                                                                                                                                                                                        aaaacgggtgtgatagaaGAACCCAGCGGGCCA‑CCCCGTTCGCC‑GAGGATAGTTAAACCGCTGGTTGACAACGGGGCTCCGGATGTCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCCGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACAcc                                                           <  2:658595/222‑1 (MQ=255)                                                                                                                                                                                              cGGTA‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGaa                                                     <  1:711980/238‑1 (MQ=255)                                                                                                                                                                                               ggggggatggaaaacccgggggcgCA‑GCCGAGTTCCC‑GATGAAAGTTGAAGCAGTGCTTGCAACCGGGGCCCCGGAGGCCGCCAGTACAGAAGCCGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTTCTTACAGCTGCGGATTCAACACCCACGaa                                                     <  2:514112/218‑1 (MQ=255)                                                                                                                                                                                                 tG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTAAACACCCACAAAAGcc                                                 >  2:159590/1‑239 (MQ=255)                                                                                                                                                                                                   gatggaaaaacccggcggcgCA‑GCCCGTTTACC‑GAGGATGGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGccc                                                <  1:117438/225‑1 (MQ=255)                                                                                                                                                                                                         agaatgcAGCGGCCAC‑GCCCAGTTATG‑TAGGATAGTTGAACCACTGATTGCAAACGCAGCACTGGAAGCCGCACTCTCAAAAGACGGATACATCCCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAgg                                           <  1:458807/232‑1 (MQ=255)                                                                                                                                                                                                         aaaaaCCAGGGGCCCA‑GCCCAGTTACC‑GAGGAAAGTTGAAGCCTTGATTGAAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATGCCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAgg                                           <  1:177551/236‑1 (MQ=255)                                                                                                                                                                                                         aGAACCCAGCGGCGCA‑GCCCAGTGACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGACCCCCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAgg                                           <  1:159590/238‑1 (MQ=255)                                                                                                                                                                                                          aaccccggcggcgCA‑CCCCGTTTACC‑GGGGAAATTGAAAGCCCTGAGTGCAAACGGAGCCCCGAAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGgca                                         <  2:292271/232‑1 (MQ=255)                                                                                                                                                                                                          aaaaCCAGGGGCGGA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGAATGCAAACGGAGCCCCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGgca                                         <  1:69325/238‑1 (MQ=255)                                                                                                                                                                                                           aaaCCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGgcag                                        >  1:814273/1‑239 (MQ=255)                                                                                                                                                                                                            aaCCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATCCACACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCACGGTTCCCAGCGGAACGGTACGTTTCGGGTGTTTAACACTACCAATACTTCAAGCTGCGGATTCACAACCCACAAAGAccccaagggaag                                       >  2:114438/1‑230 (MQ=255)                                                                                                                                                                                                                  cggcgAC‑GCCCAGTACCC‑GAGGATAGTTGAGGCACTGATTGCAAACGGAGCAGCGGAAGCCGCCATTACAGAAGTCGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGTATTCAACACCCCCGAAGGCCCACAGGCAGAGCAGaa                                 <  1:131950/239‑1 (MQ=255)                                                                                                                                                                                                                   ggggcA‑GGCCACTTTCCGGAGGATAGTGAAACTCCTGATGGAAACCGGAAACCCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGaa                                 <  2:777815/236‑1 (MQ=255)                                                                                                                                                                                                                   ggcgCA‑GGCCAGTTACC‑GAAGATAGTTAGAGCCTTGTATTCAAAGGTGACGCCAGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGGCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGaa                                 <  1:238406/238‑1 (MQ=255)                                                                                                                                                                                                                    ggccgcgCCTAGTTACC‑GAGGATAGTTGAAGCACTGCTTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGaa                                 <  1:254579/232‑1 (MQ=255)                                                                                                                                                                                                                       cA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGAACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCCGAGCAGAATACtt                            >  1:529328/1‑239 (MQ=255)                                                                                                                                                                                                                                 tACC‑GAGGATAGTTGAAACACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGAt                    <  1:749216/238‑1 (MQ=255)                                                                                                                                                                                                                                      gAGTATAGTGAAAGCGCGGATTGAAAACGGAGCACCGG‑AGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATg              <  1:312117/239‑1 (MQ=255)                                                                                                                                                                                                                                      gAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCGAAGCAGAATACTTTTAATGATCGctt               >  1:674831/1‑237 (MQ=255)                                                                                                                                                                                                                                      gAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGAACTTACAGCTGCGGATTCAACCCCAACCAAGGCCCACAGGCAAACCAAAAAAATTTTAAtaaaaccat               >  2:19600/1‑231 (MQ=255)                                                                                                                                                                                                                                       gggtgtgTTGAAGCAGTGGTGGCAAAGGGGGCACCGGAACCCGCCATTACAGAAGGCGGATACATCCCCGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATg              <  2:676002/233‑1 (MQ=255)                                                                                                                                                                                                                                        acagaGTTGAAGCCTTGTTTGACAACGGACCCCCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATCCCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATg              <  2:255691/234‑1 (MQ=255)                                                                                                                                                                                                                                         gATAGTTGAAGCACTGATTGCAAACGGAGCACCGG‑AGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAAAGCAGAAAACTTTTAATGATCGCCTGAca           >  2:312117/1‑238 (MQ=255)                                                                                                                                                                                                                                          atcgtggAAGCAATTGTTCCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGAc            <  1:227264/232‑1 (MQ=255)                                                                                                                                                                                                                                            tgTTGAACCATTGATTGCAAAAGGAGCACGGGAAGCCGCCATCACAGAAGACGGATACATACCGGAAAGCCCCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCAt         <  2:893806/238‑1 (MQ=255)                                                                                                                                                                                                                                                  aGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTCCAGCTGCGGATTCAACCCCCACGAAGGCCCACACGCAGCGCAAAAAACTTTTCATGAACCCAGGACACTCAGTgg   >  2:218829/1‑239 (MQ=255)                                                                                                                                                                                                                                                    cACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAACAGAAAAATTTTAATAAACGCATGACAAACAGAggg  >  2:323875/1‑237 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             TCATCAGAGTGATAAGGATCATCAGGGCAGTCATTTTCACTGCAGCCAGCAGCAGACCTTTTTT‑CGG‑AATACCGTTGCTGTCGACTTCACCAT‑AAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTG‑AATGCAGAAACCAGCGGCGCA‑GCCCAGTTACC‑GAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGT  >  NC_000913/4359012‑4359486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑