Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,442,011 A→C 17.0% V56G (GTG→GGG)  msrA ← methionine sulfoxide reductase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,442,0110AC17.0% 103.2 / 2.0 58V56G (GTG→GGG) msrAmethionine sulfoxide reductase A
Reads supporting (aligned to +/- strand):  ref base A (8/36);  new base C (11/2);  total (19/39)
Fisher's exact test for biased strand distribution p-value = 2.25e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGATTCTCCCAAAATACCTGTAGCAACTGCTCATAGCTGATGACGGAAGGATCGTAAACAATGCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGCGGGGGAAACCAGATGCTTTTTATCAAATAAACTCATGGTGTCGCTCTCCCGAAAATCGGTCATTGGGGTTAAGGTTGTAACAAGAGGCGTATTTGCACACAATAA  >  NC_000913/4441773‑4442246
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                             
tttcTGGGTGCCGTGGTCATTCCCGTGACCAATTCCCGGGGGGGGAGCGGGATTCTCCAAAAATCCCTGTAGCAACTGCTCAGAGCGGAAGACGGAAGGATCGTAAACAATGCGTACCGCTTCGGCAGGCCCCGTATCCCCGGAGCGCCCTTCCCGATAAGTCGGATTGGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCca                                                                                                                                                                                                                                               <  2:545274/236‑1 (MQ=255)
ttcgtggggtccggtgtttttccccTGACCATTTCCCTGGGGGGGTCTGTTATCCCCCAAAAATCCCGTTAGCAACTGCTAAGAGCGGATGACGGAAGGATCGTAAACAAGGCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACCCTTCCCGATAAGTCGGATTTGGGGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCca                                                                                                                                                                                                                                               <  2:278049/217‑1 (MQ=255)
   ctggcgctcggtgTCTTTCCCCTCACCCTTCCCCTGGGCGGGATCGTGTTTCCCCAAAAACCCTGTGAGCAACGCCTCATCGCGGATGACGGAAGGATCGTAAACAATGCTTACCGTTTCGGCATGACCCGTCTCCCCGGAGCCCCCTTCCCGATAAGGCGGATGTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCca                                                                                                                                                                                                                                               <  2:814947/224‑1 (MQ=255)
           cGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGATTCTCCCAAAATACCTGTAGCAACTGCTCATAGCTGATGACGGAAGGATCGTAAACAATGCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTCTAGCCGCCGGTATAGCCTGCGCCGGTGCTGTTAACGCCGGGTAAATGCCTGACCCCACGCTCCCCAaccccgcaa                                                                                                                                                                                                                                    >  1:369456/1‑234 (MQ=255)
           cGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGATTCTCCCAAAATACCTGTAGCAACTGCTCATAGCTGATGACGGAAGGATCGTAAACAATGCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGGAACTGCCACAACAGACGCTCCACACCCCCGaaa                                                                                                                                                                                                                                    >  1:613378/1‑239 (MQ=255)
           cGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGATTCTCCCAAAATACCTGTAGCAACTGCTCATAGCTGATGACGGAAGGATCGTAAACAATGCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGGGCTGTAAAAGCCGGGGAACTGCCAGAACAGACGCCCCACACCCCAaaaa                                                                                                                                                                                                                                    >  1:222344/1‑239 (MQ=255)
           cGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGATTCTCCCAAAATACCTGTAGCAACTGCTCATAGCTGATGACGGAAGGATCGTAAACAATGCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGGATAGCCTGCGGCGGGGCGGGTAATAGCGGGTCACTTCCGGAACAGACGCCCCCCACCCCCGaca                                                                                                                                                                                                                                    >  1:30728/1‑237 (MQ=255)
             tGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGATTCTCCCAAAATACCTGTAGCAACTGCTCATAGCTGATGACGGAAGGATCGTAAACAATGCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGGAAAAGCCGGGGAAAGGCCAGAACAGACGCGCCCACCCCCAGaaaac                                                                                                                                                                                                                                  >  1:814947/1‑237 (MQ=255)
              ggTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGATTCTCCCAAAATACCTGTAGCAACTGCTCATAGCTGATGACGGAAGGATCGTAAACAATGCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAAACCCGGGTAACTGCCAGAACAGACGCTCCCCACCCCAGAaaca                                                                                                                                                                                                                                  >  1:178484/1‑238 (MQ=255)
                            cGCATTCCCTGGGCGGGATCGTGATTCTCCCAAAATACCTGTAGCAACTGCTCATAGCTGATGACGGAAGGATCGTAAACAATGCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGGAAACGCCGGGGAACTGCCCAGAAAAAACCCCCCAACCCCCGAAACAACCCACCGCcaaaaa                                                                                                                                                                                                                   >  1:476193/1‑238 (MQ=255)
                                       ggCGGGATCGTGATTCTCCCAAAATACCTGTAGCAACTGCTCATAGCTGATGACGGAAGGATCGTAAACAATGCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCCGGTAACTGCCCGAACAGACGCCCCCCACCCCCGAAACAACCCATCGCCAAAAAGGaaacacc                                                                                                                                                                                                          >  1:185770/1‑233 (MQ=255)
                                            gttCGTGATTCCCCCAAAATACCTGTAGCAACTGCTCATAGCTGATGCCGGAAGGATCGTAAACAAGGCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACCCTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTcc                                                                                                                                                                                                    <  1:418955/236‑1 (MQ=255)
                                             aTCGTGATTCTCCCAAAATACCTGTAGCAACTGCTCATAGCTGATGACGGAAGGATCGTAAACAATGCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACACTTCCCGATCAGTCGGATTTGGCGTATAGCCGCCGGTCAAGCCTGCGGGGGGGGCGTACACCCCCGGGAACTGCCGCGACAAACGCCCCACCCCCCCCACACCAACCACCCCAAAAACAGCaaaccacacaccc                                                                                                                                                                                                   >  2:787023/1‑227 (MQ=255)
                                              ttttttttCCCCCAAATTTCCTTTAACAAGTGCCCTTGGGGGATGACGGAGGGATCGAAACCATTCCGTCCCGTTTGGGCTGGACCCGTAGCCCCGGAGCCCCCTTCCCGATAAGGCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTc                                                                                                                                                                                                 <  2:290232/233‑1 (MQ=255)
                                              tcggtatcctcCCAAAATCCCTGTAGCAACGGCCCAAAGCTGATGACGGAAGGATCGTAAACAATGCGTACCGCTTCGGCATGCCCCGTATCCCCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTc                                                                                                                                                                                                 <  1:120497/231‑1 (MQ=255)
                                              taggtaTTCTCCAAAAATCCCTGTAGCAACGGCTCATAGCTGATGACGGAAGGATCGTAAACAATGCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTc                                                                                                                                                                                                 <  1:164765/234‑1 (MQ=255)
                                               cGTGTTTCCCCCAAAATCCCTTGAGCAACTGCCCATGGCTGATGACGGAAGGATCGTAAACAATGCGTACCGCTTCGGCATGACCCGTATCCCCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTc                                                                                                                                                                                                 <  1:607535/238‑1 (MQ=255)
                                                ggtatttccccAAAATCCCTGTAACAACTGCTCATAGCTGATGACGGAAGGATCGAAACCAATGCGTACCGCTTCGGCATGACCCGTATCCCCGGAGCACCCTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAg                                                                                                                                                                                               <  1:692174/231‑1 (MQ=255)
                                                gTGATTCCCCAAAAATCCCTGTAGCAACTCACCAAGGGTGATGACGGAAGGATCGTAAACAATGGGTACCGCTTCGGCATGACCCGTATCCCCGGAGCACACTTCCCGATAAGGCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAg                                                                                                                                                                                               <  1:9207/239‑1 (MQ=255)
                                                 tGATTCTCCCAAAATACCTGTAGCAACTGCTCATAGCTGATGACGGAAGGATCGTAAACAATGCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGGGCTGTAAAACCCCGGGAACTGCCCGAACAGACACCCCCCCCCCCCCAAAAAACACAACCCcaaaaaaaaaaaacacaatccccacgg                                                                                                                                                                                              >  2:418955/1‑217 (MQ=255)
                                                      ctcCCAAAATACCTGTAGCAACTGCTCATAGCTGATGACGGAAGGATCGTAAACAATGCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCCGGGAACCTCCAGAACAGACGCTCCACACCCCAGAAACAAACCAACCCCAAAATGGCAAACTCCATTCCCTCACGGACaa                                                                                                                                                                                         >  1:772523/1‑238 (MQ=255)
                                                      ctcCCAAAATACCTGTAGCAACTGCTCATAGCTGATGACGGAAGGATCGTAAACAATGCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGAATAGCCTGCGGCGGTGCTGTGAAAGCCGGGGGAATTCCACAAAACAAGCTCCCCAACCCCGCAACAAACCAACCCAAAAAAGGCAAACCCCATTCCCCCAggaaaaa                                                                                                                                                                                         >  2:669367/1‑234 (MQ=255)
                                                      ccccaaaaaTACTTGTGGCAACTGCTCAAAGGTGATGACGGAAGGATCGAAAACAAGGGTACCGGCTTCGGCTTGCCCCGTACGACCGGAGCACCCTTCCCGAAAGGTCGGATTTGGCGTATGGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACAt                                                                                                                                                                                         <  2:277922/234‑1 (MQ=255)
                                                                     tAGCAACTGCTCATAGCTGATGACGGAAGGATCGTAAACAATGCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAAACCCGGGTAACTGCCAGACAAGACCCTCCCCACCCCCGCAAAAACCCACCCCAAAAATGGCAAACTCCATTCCCTCCgggaaatatgcacttaggtat                                                                                                                                                                           >  1:149100/1‑219 (MQ=255)
                                                                                 caaaGCTGATGACGGAAGGACCGTAAACAATGCGTCCCGCTTCGGCATGACCCGTATCCCCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCa                                                                                                                                                              <  1:873085/236‑1 (MQ=255)
                                                                                   gagttggagagggAAGTATCGTAAACAAGGCGTCCCGCTTCGGCATGCCCCGTATCACCGGAACACCCTCCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTACATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATg                                                                                                                                                            <  1:649400/228‑1 (MQ=255)
                                                                                      ttgaggaCGGAAGGATCGTAAACAAGGCGTACCGCTTCGGCATGCCCCGTATCCCCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAg                                                                                                                                                                                               <  1:741523/201‑1 (MQ=255)
                                                                                                     tcgaaaaacgggCGTCCCGCTTGGGCATGACCCGTATCCCGGGAGCACACTTCCCGAAAAGGCGGATGTGGCGTAACGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCa                                                                                                                                           <  2:178484/227‑1 (MQ=255)
                                                                                                            cAATGCGTACCGCTTGGGCATGACCCGTATCCCCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCCACAcccc                                                                                                                                                                                                                                         <  1:563270/138‑1 (MQ=255)
                                                                                                             aatggggtccGCGTCGGCTTGCCCCGTTTCCCGGGAGGCACCTGCCCGATAAGTCGGATTGGGCGTAGAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGgtgt                                                                                                                                  <  1:449982/231‑1 (MQ=255)
                                                                                                                   ccgccccTTCGGCATCCCCCGTACCCCCGGGCCCACCTTCCCGATAGGTCGGATTTGGGGTATAGCCGCCGGTATAGCCTGCGGCGGTCCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGt                                                                                                                             <  2:540303/233‑1 (MQ=255)
                                                                                                                          ttCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGGAACTGCCAGAACAGACGCTCcaca                                                                                                                                                                                                                                             >  1:786942/1‑121 (MQ=255)
                                                                                                                                 tGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCCGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGGACATTGGTCATTGGGGGACCGTTTACCGCATGCACCGTGGCTCCGGGCATCGGGGGGTTTCGTCCAGGCCGGGccct                                                                                                              >  1:540303/1‑236 (MQ=255)
                                                                                                                                   ccccGTATCCCGGGAGCACACTCCCCGATAGGTAGGATTGGGCGTAAAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATcgg                                                                                                            <  1:18960/238‑1 (MQ=255)
                                                                                                                                   aCCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACCCCCCCGAAACAAACCATCGCAAAAAAGGCAATCTCCATTCCCTCAGGGCAATTGGTCCTTGAGGGACCGTTGACCCCCTCCCACGGGGCTACGGGGATCGGGGGGTTACGGCCCGGCAGGGAACcgg                                                                                                            >  2:753780/1‑239 (MQ=255)
                                                                                                                                              cGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGCACAAACGCCCCCCAACCCAATAACCAACCACCGCCAAAATTGCCATATCCACTCCGCGCGGTACCTTGGTCCTTGCGTGACCGTTGACCGCCGCCCACGTGGCTACGGGCCACAGGGTGGTACGTCCcaggcaagccccggggggggggaaa                                                                                                  >  2:74529/1‑220 (MQ=255)
                                                                                                                                              cGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAAATGCCAGAACAGACGCCCCCCACCCCCGAAACAACCCCTCGCAAAAAAGGCCATCTCCATTCCCTCAGGGACAATTGGCCTTTAGTGAACGTTGACCCCACTCAGCGGGGCTCCGGGCAACCGGGGGGTTCGGCCAGGCGAGGCCACGGCGGGGGgaaaa                                                                                                 >  2:843334/1‑237 (MQ=255)
                                                                                                                                                           tCCCGAAAAGTCGGTTTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGCGGGGGAAACCAGATGCttttt                                                                                      <  1:368887/239‑1 (MQ=255)
                                                                                                                                                                 tagttCGGTTTGGGCGTATTGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCCAACCCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGCGGGGGAAACCAGATGCTTTTTATCaaa                                                                                <  2:222344/235‑1 (MQ=255)
                                                                                                                                                                  aaGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAAATGCCCGAAAAGACGCCCCCCACCCCCGGAAAAACCCCTCTCAAAAATGGCCATATCCATTCCGCCACGTACACTGGTCCATGGTGGCACATTGACCACATACAGCGTGGCTACGGGCATCGGGGGGTTACGTCCCGGCAGGGGCTCCGCgggggggaacacagggctttttttaata                                                                               >  2:872675/1‑216 (MQ=255)
                                                                                                                                                                   acgcgtaTTTGGCTATAAGCCGGCGGTTAAGCCTGGGGCGGTGCTGTAAACGGCGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGGAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGCGGGGGAAACCAGATGCTTTTTATCAAATa                                                                              <  1:92413/233‑1 (MQ=255)
                                                                                                                                                                                    caGCCGCCTGTATAGCCTGCGGCGGGACTGGAAACGACGGGTAACCGCCAGAACAGACCCCTCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTTACCGCATTCAGCGTGGCTACTGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGCGGGGGAAACCAGATGCTTTTTATCAAATAAACTCATGGTGTCGct                                                              <  1:101353/238‑1 (MQ=255)
                                                                                                                                                                                         gccgGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGCGGGGGAAACCAGATGCTTTTTATCAAATAAACTCATGGTGTCGCTCTccc                                                         <  1:330430/239‑1 (MQ=255)
                                                                                                                                                                                                       gggccggtcttgaacacccGGTTAACTGCCAGAACAGACGCGCCACACCCCAAAAACAACCCAACGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGGCATTTAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGCGGGGGAAACCAGATGCTTTTTATCAAATAAACTCATGGTGACGCTCACCCGAAGTACGGTCAtt                                           <  2:30728/222‑1 (MQ=255)
                                                                                                                                                                                                        gggggctcTGTAAACGCCGGGTAACTGCCAGAACAGACGGTCCACACCCCCGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGCGGGGGAAACCAGATGCTTTTTATCAAATAAACTCATGGTGTCGCTCTCCCGAAAATCGGTCAtt                                           <  1:859404/231‑1 (MQ=255)
                                                                                                                                                                                                              tcgtaaaaCGCCGGGTAACTTCAAGAAACGACGCTCCACACCCCAGAAAAAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGTGGGGGAAACCAGATGCTTTTTATCAAATAAACTCATGGTGTCGCTCTCCCGAAAATCGGTCATTGGGGtt                                     <  2:369456/233‑1 (MQ=255)
                                                                                                                                                                                                              gccgTAAACGCCGGGTAACCGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGCGGGGGAAACCAGATGCTTTTTATCAAATAAACTCATGGTGTCGCTCTCCCGAAAATCGGTCATTGGGGtt                                     <  1:278054/235‑1 (MQ=255)
                                                                                                                                                                                                              gCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCCACCCCCCAGAAACTACCCATCGCAAAAATGGCAATCGCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGCGGGGGAAACCAGATGCTTTTTATCAAATAAACTCATGGTGTCGCTCTCCCGAAAATCGGTCATTGGGGTTa                                    <  1:74529/239‑1 (MQ=255)
                                                                                                                                                                                                              gCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGCGGGGGAAACCAGATGCTTTTTATCAAATAAACTCATGGTGTCGCTCTCCCGAAAATCGGTCATTGGGGtt                                     <  1:614974/238‑1 (MQ=255)
                                                                                                                                                                                                               cTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGCGGGGGAAACCAGATGCTTTTTATCAAATAAACTCATGGTGTCGCTCTCCCGAAAATCGGTCATTGGGGTTa                                    <  1:753780/238‑1 (MQ=255)
                                                                                                                                                                                                                         cGGGTAACTGCCAGAACAGACGTTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGt                                                                                                                             <  1:363772/140‑1 (MQ=255)
                                                                                                                                                                                                                         cGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCATCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGt                                                                                                                             >  2:363772/1‑140 (MQ=255)
                                                                                                                                                                                                                          gggTAAATGCCAGAAGAGACGGTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCAGTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGCGGGGGAAACCAGATGCTTTTTATCAAATAAACTCATGGTGTCGCTCTCCCGAAAATCGGTCATTGGGGTTAAGGTTGTAACa                         <  1:152713/238‑1 (MQ=255)
                                                                                                                                                                                                                              tcccgCCAGAACAGCCGCTCCCCCCCCCACAAACAACCCCTCGCAAAAATGGCAATCCCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGCGGGGGAAACCAGATGCTTTTTATCAAATAAACTCATGGTGTCGCTCTCCCGAAAATCGGTCATTGGGGTTAAGGTTGTAACAAGAgg                    <  2:774664/235‑1 (MQ=255)
                                                                                                                                                                                                                                        aaCAGGCGCTCAGCACCCCAGAAACAAGCCATCGCAAAAAAGGCCATCCCCAATCCGTCAGGTACATTGGTCCATGAGTGCCCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGCGGGGGAAACCAGATGCTTTTTATCAAATAAACTCATGGTGTCGCTCTCCCGAAAATCGGTCATTGGGGTTAAGGTTGTAACAAGAGGCGTATTTGc           <  1:138327/239‑1 (MQ=255)
                                                                                                                                                                                                                                        aaCAGCCGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCCCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGCGGGGGAAACCAGATGCTTTTTATCAAATAAACTCATGGTGTCGCTCTCCCGAAAATCGGTCATTGGGGTTAAGGTTGTAACAAGAGGCGTATTTGc           <  1:843334/239‑1 (MQ=255)
                                                                                                                                                                                                                                         aCAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGCGGGGGAATCCAGATGCTTTTTATCAAATAAACTCATGGTGTCGCTCTACCGAAAATCGGTCATTGGGGTTAAGGTTGTAACAAGAGGCGTATTTGc           <  1:578886/238‑1 (MQ=255)
                                                                                                                                                                                                                                         aCAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGCGGGGGAAACCAGATGCTTTTTATCAAATAAACTCATGGTGTCGCTCTCCCGAAAATCGGTCATTGGGGTTAAGGTTGTAACAAGAGGCGTATTTGca          <  1:147092/239‑1 (MQ=255)
                                                                                                                                                                                                                                         aCAGACGCTCCAAACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGCGGGGGAAACCAGATGCTTTTTATCAAATAAACTCATGGTGTCGCTCTCCCGAAAATCGGTCATTGGGGTTAAGGTTGTAACAAGAGGCGTATTTg            <  1:49419/237‑1 (MQ=255)
                                                                                                                                                                                                                                          ccgccccTCCCCCCCCCAAAAAAACCCCATGCAAAAAATGGCAATCCCCATTCGGGGAGGTCAATTGGTCTTTGAGTGACCGTTGGCCGCATGCAGCGTGGGTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGCGGGGGAAACCAGATGCTTTTTATCAAATAAACTCATGGTGTCGCTCTCCCGAAAATCGGTCATTGGGGTTAAGGTTGTAACAAGAGGCGTATTTGcac         <  2:843086/233‑1 (MQ=255)
                                                                                                                                                                                                                                              cccTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCCCCATTCCGTCAGGTACATTGGTCATTGAGTTACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGCGGGGGAAACCAGATGCTTTTTATCAAATAAACTCATGGTGTCGCTCTCCCGAAAATCGGTCATTGGGGTTAAGGTTGTAACAAGAGGCGTATTTGcac         <  1:551157/233‑1 (MQ=255)
                                                                                                                                                                                                                                                 cccACCCCCCAGAAACAACCCAACGCAAAAATGGCAATCCCCATTCCGGCAGTTACATTGGTCATTGAGTGACCGTTGACCGCAGGCAGCGGGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGCGGGGGAAACCAGATGCTTTTTATCAAATAAACTCATGGTGTCGCTCTCCCGAAAATCGGTCATTGGGGTTAAGGTTGTAACAAGAGGCGTATTTGCACACAATaa  <  1:54887/238‑1 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                             
ATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGATTCTCCCAAAATACCTGTAGCAACTGCTCATAGCTGATGACGGAAGGATCGTAAACAATGCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGCGGGGGAAACCAGATGCTTTTTATCAAATAAACTCATGGTGTCGCTCTCCCGAAAATCGGTCATTGGGGTTAAGGTTGTAACAAGAGGCGTATTTGCACACAATAA  >  NC_000913/4441773‑4442246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: