Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 48,555 A→G 18.7% S263G (AGC→GGC)  kefC → potassium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091348,5550AG18.7% 96.6 / 2.2 47S263G (AGC→GGC) kefCpotassium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base A (7/29);  new base G (7/3);  total (15/32)
Fisher's exact test for biased strand distribution p-value = 4.56e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGGCGTTAAAAGTGGCGGGTGCGCTGGTGCTGGTGGTATTGCTGGGGCGCTATGTCACGCGTCCGGCGCTGCGTTTTGTAGCCCGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGGGCGCGTTTCTGGCGGGCGTACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCATCGGTGTTGGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTAGGGCAGGGCAGTGAGTTTGCCT  >  NC_000913/48318‑48786
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                             
tgggggTTAAAAGGGGGGGGTGCGCTGGGGCTGGTGGTATTGCTGGGTTGGTATGTCACGCGTCCGGCGCTGCGTTTTGTAGCCCGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGGGCGCGTTTCTGGCGGGCGTACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGagag                                                                                                                                                                                                                                              <  1:893373/234‑1 (MQ=255)
tcGGCGTTAAAAGTGGCGGGTGCGCTGGTGCTGGTGGTATTGCTGGGGCGGTATGTCACGCGTCCGGCGCTGCGTTTTGTAGCCCGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGGGCGCGTTTCTGGCGGGCGTACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGagag                                                                                                                                                                                                                                              <  1:697404/239‑1 (MQ=255)
         aaaGGGGCGGGTGCGCTGGGGCTGGTGGTATTGCTGGGGCGCTATGTCACGCGTCCGGCGCTGCGTTTTGTAGCCCGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGGGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGGGCGCGTTTCTGGCGGGCGTACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGa                                                                                                                                                                                                                                     <  1:713413/239‑1 (MQ=255)
          aaGTGGCGGGTGCGCTGGTGCTGGTGGTATTGCTGGGGCGCTATGTCACGCGTCCGGCGCTGCGTTTTGTAGCCCGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGGGCGCGTTTCTGGCGGGCGTACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGaa                                                                                                                                                                                                                                    <  1:679041/239‑1 (MQ=255)
          aaGTGGCGGGTGCGCTGGTGCTGGTGGTATTGCTGGGGCGCTATGTCACGCGTCCGGCGCTGCGTTTTGTAGCCCGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGGGCGCGTTTCTGGCGGGCGTACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGaa                                                                                                                                                                                                                                    <  1:515293/239‑1 (MQ=255)
          aaGGGGGGGGTGCGCTGGTGCTGGTGGTATTGCCTGGGCGCTATGTCACGCGTCCGGCGCTGTGTTTTGTAGCACGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGGGCGCGTTTCTGGCGGGCGTACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGaa                                                                                                                                                                                                                                    <  1:482963/239‑1 (MQ=255)
          aaGGGGCGGGTTCGCTGGTGCTGGTTGTATTGCTGGGGCGCTATGTCACGCGTCCGGCGCTGCGTTTTGTAGCCCGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGGGCGCGTTTCTGGCGGGCGTACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGGGCGATATCGaa                                                                                                                                                                                                                                    <  1:483725/239‑1 (MQ=255)
          aaGGGGCGGGTGCGGTGGTGCGGGTGGTATTGCTGGGGCGCTATGTCACGCGTCCGGCGCTGCGTTTTGTAGCCCGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGGGCGCGTTTCTGGCGGGCGTACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGaa                                                                                                                                                                                                                                    <  1:868974/239‑1 (MQ=255)
                   gTGCGCTGGTGCTGGTGGTATTGCTGGGGCGCTATGTCACGCGTCCGGCGCTGCGTTTTGTAGCCCGCTCTGGCTTGCGGGAAGTGTTTAGGGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGGGCGCGTTTCTGGCGGGCGTACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGAACCATTTaa                                                                                                                                                                                                                            <  1:825501/238‑1 (MQ=255)
                                ggtggtATTGCTGGGGCGCTATGTCACGCGTCCGGCGCTGCGTTTTGTAGCCCGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGGGCGCGTTTCTGGCGGGCGTACTGCTGGCCAGCTCGGGATACCGTCATGGGCTGTGGCGGGGGATCGAACCATTTTCAGGTTTTCGGTTgg                                                                                                                                                                                                            >  1:872382/1‑239 (MQ=255)
                                             gggtcgtttGTCAGGGGTTCGGGGCTGGGTTTTTGACCCCGCTTTGGTTTGCGGGAATGGTTTAGTGCAGTGGCGTTATTCCTGGTGTTTGGGTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGGGCGCGTTTCTGGCGGGCGTGCTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCAt                                                                                                                                                                                               <  2:212942/231‑1 (MQ=255)
                                              ggCGGTATGGTACGCGTCCGGGGCTGCGTTTTGTAGCCCGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGGGCGCGTTTCTGGCGGGCGTACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCAt                                                                                                                                                                                               <  1:474627/238‑1 (MQ=255)
                                               gcgcTATGTCACGCGTCCGGCGCTGCGTTTTGTAGCCCGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGGGCGCGTTTCTGGCGGGGGTACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGGGGGCGGTATCGAGCCATTTAAAGGTTTTCTGTTGGGGCTGttttttctcg                                                                                                                                                                                             >  1:390248/1‑234 (MQ=255)
                                                   tttgtcccggttCCGGCTCTGCGTTTTGTAGCCCGGTCTGGCTTGCGGGAAGGGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGGGCGCGTTTCTGGCGGGCGTACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCATCGgtg                                                                                                                                                                                          <  1:356112/227‑1 (MQ=255)
                                                        cccgcgTCGGGTGTTGGTTTTTTTAGCCCGCTTTGTGTTGCGGGAAGTGTTTAGGGCCGTGGCGTTATTCCTCGTGGTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGGCGATGGCGATGGGCGCGTTTCTGGCGGGCGTACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCATCGGTGTTGGCa                                                                                                                                                                                    <  2:390248/237‑1 (MQ=255)
                                                         aCGCGTCCGGTGCTGCTTTTTGGTGCCCGTTTTGGCTTGCGGGAAGTGTTTAGGGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGGGCGCGTTTCTGGCGGGCGTACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCATCGGTGTTGGCa                                                                                                                                                                                    <  1:378236/238‑1 (MQ=255)
                                                                 ggCGCTGCGTTTTGTAGCCCGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGGGCGCGTTTCTGGCGGTCGGGCCGGTGGGCACCTCGGCGTTACGCCATTCCCCGGGGAGCGGGCTCGAACCCATTTAACGTGTTCTGTTGGGGGTGTTTTTCTTCGGTGGTGGcgttgctgtag                                                                                                                                                                           >  2:4704/1‑229 (MQ=255)
                                                                                            atggCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGGGCGCGTTTCTGGCGGGCGTACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCATCGGTGTTGGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCa                                                                                                                                             <  1:814536/235‑1 (MQ=255)
                                                                                                                   tGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGGGCGCGTTTCTGGCGGGGGGAGTGCTGGCGAGCTCGGGGAAACGGCCTTCGCTGGGGGGCGGTAACGGACCATTTTAAGGTTTGTCTGTGGGGGCGTTTTTTATCGGGGTTTGCATGTTGGTAGAACTTTGGCCGCTGCTTGTAAAACCCCTTgcgagtttgattttttgggcc                                                                                                                      >  2:483763/1‑221 (MQ=255)
                                                                                                                   tGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGGGCGCGTTTCTGGCGGGCGTACTGCTGGGAAGCTCGGAATACCCGCAAGGGCTGGGGGGGCGGATCTAACCACTTAAAGGTTTTCGTGTGGGGGCGTTTTTTCTCTGTGTTTGGGTGTCGGTAAGATTTTGGACGCCGTTTGGAAAACCCTTTGGCATTGTCTTTTttctgggc                                                                                                                     >  2:680970/1‑236 (MQ=255)
                                                                                                                                cGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGGGCGCGTTTCTGGCGGGGGGACTGCTGGCCAGCTCGGCAATACGTCATGGGCTGGCGGGCGGTGTCGTACCATTTTACAGGTTGCGTTTGGGGGGGTTTTTTATCGGGTTTTGGATGTGGTAAAACTTTGTCACGCGGCTTGTAAAACCATTTTGCACTGTCAttttttttctcgtgtttctcttca                                                                                                        >  2:162499/1‑219 (MQ=255)
                                                                                                                                     ttGGCTTTGGGTTGCGGCGGGAAGAGGTCGGCTTGTCGATGGGGATGGGCGCTTTTCGGGCGGGGGTACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATAGCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCATCGGTGTTGGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCaa                                                                                                    <  1:786600/238‑1 (MQ=255)
                                                                                                                                      tGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGGGCGCGTTTCTGGCGGGGGTGATGCTGGCCAACCTGGGGATACGTCCTGTGGTGGGGGGGGTGATCGCGCCCATTTAAGGGTTGGAGTTGGGGGTGTTTTTTCTTCGTGTTGGCATTGCGATAGAGTTTGTCACGCCGCTTGATAAACCCCTGTGCGTTTTCATTTTTCTGGTCGGGTTCCTCCTCATCaaaa                                                                                                  >  2:807953/1‑239 (MQ=255)
                                                                                                                                                        ggAAGAGGTCGGCTTGTCGATGGCGATGGGCGCGTTTCTGGCGGGCGTACTGCTGGCCACCTCGGCATACCGTCATGCTCTGGGGGGCGATATCGAAACCTTTTAAGGTTTGGTGGTGGGGGTGTTTTTTCTTGTGGTTGGGATTTCGCAAAACTTTGTCACGCCGCTTGTAAACCCCTTTCGCATTGTCGTTTTTTTTCCCGGTTTTCCCATAATCAAAAACGCCCAGCTgggggggt                                                                                >  2:724327/1‑235 (MQ=255)
                                                                                                                                                                       ggcggtgcgctggggCGCGTTGTGGGCGGGGGTTCTGCTGGCAAGTCTGGAATGCCGTCGTGGCTTGGAGAGCGATTTCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCATCGGTGTTGGCATGTCGACAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGc                                                                 <  2:872382/227‑1 (MQ=255)
                                                                                                                                                                         cgatggcgatggGCGCGTTTCTGGCGGGCGTACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATAGCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCATCGGTGTTGGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCaa                                                               <  1:888772/239‑1 (MQ=255)
                                                                                                                                                                            tggcgatggGGGCGTTTCTGGCGGGCGTACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCATCGGTGTTGGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGt                                                             <  1:680970/238‑1 (MQ=255)
                                                                                                                                                                            tggcgatggGCGCGTTTCTGGCGGGCGTACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCATCGGTGTTGGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGt                                                             <  1:162499/238‑1 (MQ=255)
                                                                                                                                                                                  tggGCGCGTTTCTGGCGGGCGTACTGCTGGCCACCTCGGTATACCGCCATGCGCTGGTGGGCGATATCGAACCAATTTACGGGTTTCTGTTTGGGCTTTTTTTCTTCGGTGGTGGCATTGTGGTAGACTTTGACACCCGGCTTTCAAACCCATTTGCCCTTGTCATTTTGTTGCTGGGGTGGCTCACACTCAAACTCGCCATGCGTGGGCTGTTTgacagccccgagaaagggcagaac                                                      >  2:713944/1‑216 (MQ=255)
                                                                                                                                                                                                     cgTACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGGGAGCGGTATCGAACCATTTAAAGGGTTGCTGTTGGGGCCGTTTTTCATCGGTGTTGGCATGTCGATAGAGTTTGGCACGCTGCTTGAAAACCCATTGCGCATTTTCATTTTGCTGCTCGGTTTCCTCATCCTCCAAATCGCCATGCTGTGGCTGATTGCCCCACCCGTGCAAGAGCaaaaaaaaaacagcgggtggtttg                                   >  2:194612/1‑223 (MQ=255)
                                                                                                                                                                                                     cgTACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCATCGGTGTTGGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTttctgc                                                                                                                       >  1:854364/1‑155 (MQ=255)
                                                                                                                                                                                                     cgTACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCATCGGTGTTGGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTtgctgc                                                                                                                       <  2:854364/155‑1 (MQ=255)
                                                                                                                                                                                                      gTACTGCTGGCAAGCTCGGAATTCCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCATCGGTGTTGGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTg                                   <  1:807953/238‑1 (MQ=255)
                                                                                                                                                                                                      gTACTGCGTGCAATCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCATCGGTGTTGGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTg                                   <  1:590440/238‑1 (MQ=255)
                                                                                                                                                                                                         ctggtgGCAAGCGCGGAATTCCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCATCGGTGTTGGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGgt                               <  1:483763/239‑1 (MQ=255)
                                                                                                                                                                                                         ctgctgGCAAGCTCGGAATGCCGTCATGCGCTGGAGGGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCATCGGTTTTGGCATGTCGATAGACTTTGGCTCGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGgt                               <  1:842954/239‑1 (MQ=255)
                                                                                                                                                                                                         ctgctgGCAAGCGCGGAATACCGTCATGCGCTGGAGGGAGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCGGTTTTTCATCGGTGTTGGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCAGTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGgt                               <  1:497132/239‑1 (MQ=255)
                                                                                                                                                                                                          tgcttGCAAGCTTGGAATTCCGTCATGCGCTGGAGAGCGATTTCGAACCATTTAAAGGTTTTCTGTTGGGGCTGTTTTTCATCGGTGTTGGCATGTCGATAGACTTTGGCACGCTGCTTGAAACCCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGgt                               <  1:63503/238‑1 (MQ=255)
                                                                                                                                                                                                                     cTCGGAATACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCATCGGTGTTGGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCa                                                                                                                                             <  2:350578/118‑1 (MQ=255)
                                                                                                                                                                                                                     cTCGGAATACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCATCGGTGTTGGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCa                                                                                                                                             >  1:350578/1‑118 (MQ=255)
                                                                                                                                                                                                                     cTCGGAATACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCATCGGTGTTGGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCCTCTGTGGGTTGGGGGGTGGTGagggc                    >  2:775964/1‑239 (MQ=255)
                                                                                                                                                                                                                       tcggtttACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTGTCTGTGGGGGCTGTTTTTCATCGGTGTTGGCATGTCGATAGAGTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTagggc                    <  1:310008/232‑1 (MQ=255)
                                                                                                                                                                                                                       cGGAATACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCATCGGTGTTGGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGGGTTGTTagggcag                  >  1:103583/1‑239 (MQ=255)
                                                                                                                                                                                                                             ttccGTCATGCGGTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCATCGGTGTTGGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTAgggcagggca              <  1:339238/236‑1 (MQ=255)
                                                                                                                                                                                                                             tACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCATCGGTGTTGGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTAgggcagggca              <  1:4704/238‑1 (MQ=255)
                                                                                                                                                                                                                                   cATGCGTTGGAGAGGGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCATCGGTGTTGGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCAATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTAGGGCAGGGCAGTGAGtt       <  1:748220/239‑1 (MQ=255)
                                                                                                                                                                                                                                     tGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCATCGGTGTTGGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAg                                                <  1:280962/196‑1 (MQ=255)
                                                                                                                                                                                                                                     tGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCATCGGTGTTGGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAg                                                >  2:280962/1‑196 (MQ=255)
                                                                                                                                                                                                                                         cTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCATCGGTGTTGGCATGTCGATAGACTTTGGCACGGTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGGCGTTGGTTTGCGGTGTTGTTAGGGCAGGGCAGTGAGTTTGCCt  <  1:213075/238‑1 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                             
TCGGCGTTAAAAGTGGCGGGTGCGCTGGTGCTGGTGGTATTGCTGGGGCGCTATGTCACGCGTCCGGCGCTGCGTTTTGTAGCCCGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGGGCGCGTTTCTGGCGGGCGTACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCATCGGTGTTGGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTAGGGCAGGGCAGTGAGTTTGCCT  >  NC_000913/48318‑48786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: