Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,058,486 | T→C | 19.2% | S135P (TCG→CCG) | torC → | trimethylamine N‑oxide (TMAO) reductase I, cytochrome c‑type subunit |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,058,486 | 0 | T | C | 19.2% | 120.7 / 6.5 | 70 | S135P (TCG→CCG) | torC | trimethylamine N‑oxide (TMAO) reductase I, cytochrome c‑type subunit |
Reads supporting (aligned to +/- strand): ref base T (38/14); new base C (2/12); total (41/29) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.03e-04 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GTGTATGAAGAATATAAACAGTCGGTGCATTTCCAGAA‑CGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTCATATCCCGCCGGATA‑TTCCAGGCATGGTGAAGCGCAAACTGG‑AAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGACACGCTGTACTCTA > NC_000913/1058252‑1058708 | gtgtATGAAGAATATAAACAGTCGGTGCATTTCCAGAA‑CGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTCATATCCCGCCGGATA‑TTCCAGGCATGGTGCAGCGCAAACTGG‑AAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGTTAAACCTGAAAAATTCCAACCCAAACGC‑GCGGAACTTGCCGAGCCTGATTGGGCGCGAAT‑ACAAGAAAACA‑ACTCGGc > 1:538806/1‑239 (MQ=255) gtgtATGAAGAATATAAACAGTCGGTGCATTTCCAGAA‑CGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTCATATCCCGCCGGATA‑TTCCAGGCATGGTGAAGCGCAAACTGG‑AAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGC‑CAGGACATTGCCGAACGTGACTGGGCGCGAAT‑GAAAGAAAACA‑ACTCgcg > 1:871094/1‑237 (MQ=255) atatAAACAGTCGGTGCATTTCCAGAA‑CGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTCATATCCCGCCGGATA‑TTCCAGGCATGGTGAAGCGCAAACTGG‑AAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACACGGCACCCGGCCGc > 1:203115/1‑238 (MQ=255) atatAAACAGTCGGTGCATTTCCAGAA‑CGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTCATATCCCGCCGGATA‑TTCCAGGCATGGTGAAGCGCAAACTGG‑AAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAAAAA‑AACCGGCAACCGGCCGc > 2:726531/1‑238 (MQ=255) tataAACGTTCGGTGC‑TTTTCAGAACCGCCTCGGGCGGGCGGGCGAAATGCCATGACTGTCATACCCCGCCGGATA‑TTCCAGGCATGGGGAGGCCAAACCTGA‑AAGGAAGAAATAATATCTACCAGCCCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCAAACCGC‑GGGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACTCGGCACCCTGCCGCTc < 2:4340/239‑1 (MQ=255) ataAACGGGGGGTTCATTTCCAGAC‑CCCCTCCGGCGTGCGAGCTAAATGCAATGCCTGTAATATCCCGCCGGATA‑TTCCAGGCATGGGGAAGCGCAAACGGA‑AAGGGGACAATGATAACTCCCAGCCCTTTATTGCTCACCCCATTGATACACCGGAAAAATTCGAAGCCAAACGC‑GGGGAACTTGCCGGGCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACCCGGCACCCTGCCGcccc < 2:256085/239‑1 (MQ=255) aCAGTCGGTGCATTTCCAGAA‑CGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTCATATCCCGCCGGATA‑TTCCAGGCATGGTGAAGCGCAAACTGG‑AAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACTCGGCAACCTGCCGCTCCTGcc > 1:800379/1‑239 (MQ=255) gTCGGTGCATTTCCAGAA‑CGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTCATATCCCGCCGGATA‑TTCCAGGCATGGTGAAGCGCAAACTGG‑AAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACCCGGCAACCTGCCGCTCCTGCCATa > 1:154785/1‑239 (MQ=255) gTCGGTGCATTTCCAGAA‑CGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTCATATCCCGCCGGATA‑TTCCAGGCATGGTGAAGCGCAAACTGG‑AAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCCCGAAT‑GAAAGAAAACA‑ACTCGGCAACCCGCCGCCCCTGCCATa > 2:122203/1‑239 (MQ=255) gTCGGTGCATTTCCAGAA‑CGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTCATATCCCGCCGGATA‑TTCCAGGCATGGTGAAGCGCAAACTGG‑AAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACCC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGACT‑GAAAGAAAACA‑ACTCGGCAACCTGCCGCTCCTGCCAtc > 1:174589/1‑238 (MQ=255) ggTGCATTTCCAGAA‑CGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTCATATCCCGCCGGATA‑TTCCAGGCATGGTGAAGCGCAAACTGG‑AAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGATTGGGCCGGAAT‑GAAAAAAAACA‑ACTCGGCAACCTGACGCACCCGCCATaaaa > 2:294726/1‑237 (MQ=255) cATTTCCAAAA‑CGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTCATACCCCGCCGGATA‑TTCCAGGCATGGTGAAGCGCAAACTGG‑AAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACTCGGCAACCTGCCGCTCCTGCCATAACTAcg < 1:122203/238‑1 (MQ=255) ccccggggtgggtgtgaagtcccATGCCTGTAATATCCCGCCGGATT‑TCCCGGGCATGGGAAACCCAAAACTGG‑AAGCGAGAAATGATATCTACCAGACCTTTATTGCGCACTCCATTGATACCCCTGAAAAATTCGAAGCAAAACGC‑GCGGAATTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACTCGGCACCCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATg < 2:392546/218‑1 (MQ=255) ccGGCGGGCGGTTTGAATGCCCTGCCTGACATATCCGCCCGGAAA‑TTCCAGGCATGGTGAAGCGCAAACTGG‑AAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGC‑GAGGAACTTGCCGAGCGTGAAGGGGCGCGAAT‑GAAAGAAAACA‑ACCCGGCACCCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCg < 1:294726/239‑1 (MQ=255) tGCGAGCTGAATGCCATGACTGTCATATCCCGCCGGATA‑TTCCAGGCATGGTGAAGCGCAAACTGG‑AAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAagcag > 1:392546/1‑239 (MQ=255) tGCGAGCTGAATGCCATGACTGTCATATCCCGCCGGATA‑TTCCAGGCATGGTGAAGCGCAAACTGG‑AAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAACCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAACCA‑ACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAagcag > 1:662563/1‑239 (MQ=255) cGAGCTGAATGCCATGACTGTCATATCCCGCCGGATA‑TTCCAGGCATGGTGAAGCGCAAACTGG‑AAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGa > 1:765410/1‑224 (MQ=255) cGAGCTGAATGCCATGACTGTCATATCCCGCCGGATA‑TTCCAGGCATGGTGAAGCGCAAACTGG‑AAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAagcagc > 1:824582/1‑238 (MQ=255) ggttaattgccctgacgtgcaatCCCGCCGGGTATTTCCAGCCGTGGGAAAGCAAAAACGGA‑AAGGGTACAAGAATATTTACCAACCCTTTATTGCCCACCCCATTGATACCCCTGAAAAAGTCGAAGCAAAACGC‑GCGGAACTTGCCGGGCGTGAAGGGGCGCGAAG‑GAAAGAAAACA‑ACCCGGCAGCCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATc < 2:869292/218‑1 (MQ=255) cttccATTACTTTCATCTCCCGCCGATTT‑TCCCAGGAAGGGTGAAGCAAAAACTGT‑AAGCGAGCAATAATATCCACCAGACTTATATGGCTCACTCCATCGATCACCCTGAAAAATTAGAACCCAAACGG‑GCGCACCTTGCCGAGCGTGAAGGGGAGCAAAA‑GAACGAAAACA‑ACTCGCCACCCCCCTGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAag < 2:487258/236‑1 (MQ=255) gtttGTCCTACCCCGCGGGATA‑TTCCAGGCATGGTGAAGCGCAAACTGG‑AAGCGAGAAATGATATCCACCAGACCTTTATGGCTCACTCAATTGATACACCTGAAAAATTCGAAGCAAAACGC‑GCGAAACTTGCCGGGCGTGAATGGGCGAGAAT‑GAAAGAAAACA‑ACTCGGCACCCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACg < 2:897240/236‑1 (MQ=255) cTGTCATATCCCGCCGGATA‑TTCCAGGCATGGTGAAGCGCAAACTGG‑AAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GATAGAAAACA‑ATTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGTACCTCGGAAGCCGCACCCTGAAGAGGCACGTc > 1:31988/1‑239 (MQ=255) cATATCCCGCCGGATA‑TTCCAGGCATGGTGAAGCGCAAACTGG‑AAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGCTGGATCATGCGAACCAGCATCCTGAAGCAGCACGTCAGAt > 1:450797/1‑239 (MQ=255) ccgGATA‑TTCCAGGCATGGTGAAGCGCAAACTGG‑AAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAAATAAAGggggg > 2:871632/1‑238 (MQ=255) gCATGGTGAAGCGCAAACTGG‑AAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GCAAGAAAACA‑ACTCGCCAACCTGCCGCTCCTGCCATAACTACGATGCGAGGGAGCATGCGAACCAGAACACTCAAGCACCACGCAGGAGGAAGGGGGGAGGGAAAGaaaaa > 2:465372/1‑238 (MQ=255) gCATGGTGAAGCGCAAACTGG‑AAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATaa > 1:842668/1‑238 (MQ=255) gCATGGTGAAGCGCAAACTGG‑AAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACTCGGCAACCTCCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATACTTAAGCAGCACGTCAGGTGTAGGGAGCAGCGAAAGATaat > 1:842674/1‑239 (MQ=255) cAAACTGG‑AAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCg > 1:456536/1‑239 (MQ=255) aCTGGAAAGCGAGAAATAATATCTCCCAGCCCTTTTTTTCTCATCCCATTGATACACCTGAAAAATTCAAACCAAAACGC‑GCGGACGTGGCGGGGCGTGAAGGGGCGCGAAA‑AAAAGAAAACA‑ACTCGGCAACCGGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGAc < 2:203115/239‑1 (MQ=255) aGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAACCA‑ACTCGGCAACCTGCCGCTCCTGCCATAACTACGCTGCGATGGACCATCCGAAGCAGCATCCTGAACCAGCCCGTCAGATGCAAGGGGCAGCGAAAGGAAATCAAACCGACATCggcgttaataaggg > 2:518299/1‑226 (MQ=255) cAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACTCGGCAACCTGCCGCTCCTGCCATAACTAcg > 1:624729/1‑143 (MQ=255) cAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACTCGGCAACCTGCCGCTCCTGCCATAACTACGTGGCGATGGATCATGCGAAGCAACATCCTGCAGCAGCCCGCCGGCTGAAGGTGGCAGCGAAAGATAATCAAAccctcaccgcacgaccaaaaggaa > 2:797006/1‑217 (MQ=255) tataagaaccagcttttATTGCTCACTCCATTGAACCCCCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACTCGGCAACCGGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGcc < 1:784899/225‑1 (MQ=255) aatCCACCAGGCCTTTATTGCTCACTCCATTGAAACCCCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCTGGAATGGGCGCGAAT‑GAAAGAAAACA‑ACGCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGc < 1:726531/236‑1 (MQ=255) aCCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACTCAGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGCTGGCAGCGAAAGATAATCAAGCCTGCATCAACTGTCATAAAGGGATTGCcaacccg > 1:854315/1‑237 (MQ=255) gccctttattccctcttCCTTTGATACACTTAAAAAATTCGAACACAAAAGC‑GGGGAACTCGCGGAGCTGGAATGGGCGAAAAT‑GAAAAAAAACA‑CCCCGGCAACCGGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTAc < 2:456536/223‑1 (MQ=255) aCCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGCCTGTCATAAAGGAATTGCCCACCAGTTAcc > 1:534168/1‑239 (MQ=255) caaccgcaatgaaacaacCTGAAAAATGAGAGGCAAAACGC‑GTGAAACTTGCCGAGCGATAATGGGC‑AGAATGGAAAGAAACAA‑TCTCGGAAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCGCCAGTTACCGGATATGAGt < 1:137999/223‑1 (MQ=255) tCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCCGATGAAGGTGCCAGCGAAAGATAATCAATCCTCCATCGCCTGTCAAAAAGGAATTGCCCCCCAGTTACCGGAAATGAgaa > 2:900990/1‑237 (MQ=255) cACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAACAGCATCCTGAAGCAGCACGTAAGATGAAGGGGGCCGCGAAAGATAATCAATCCTGCATCGACTGCCATAAAGGAATTGCCAACCAGTTAccccgtcccttata > 2:784899/1‑227 (MQ=255) tctagtagtACACCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAAAAAACA‑ACGCGTCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCgg < 1:115657/231‑1 (MQ=255) tCCATTGATACACCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACTCGGCAACCTGCCGCTCCTGCCATCACTACGATGCGATGGATCATGCGAACCAGCATCCTGACGCAGAACGTCCGTGGAAGGTGGCAGGGAAAGATAATCAATCCTGCATCACCTGTCATAAAGGAATTTCCAACCAGTTACCGGATATCAGAAACgg > 2:723751/1‑239 (MQ=255) aTACACCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GACAGAAAACA‑ACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATCTGAGTACGGGCTTCCGt > 1:345353/1‑239 (MQ=255) tACACCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAgg > 1:138324/1‑198 (MQ=255) tACACCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCCCCAGTTACCGGATATGAGTAGCGGCTTCCGTa > 1:413012/1‑239 (MQ=255) ccccTAAAAAATTCAAAG‑CAAACGCGGCGAAACTTGCCGACGGTGAATGGGCCGGAAT‑GAAAGAAACCA‑ACCCGGAAACCTGCCGTCCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAg < 1:358177/237‑1 (MQ=255) cacCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGCTGAAGGCGGCAGCGAAAGATAATCAATCCTTCATCGACTGTCATAAAGGTATAGCCCACACGTTACAGGATATGAGTAGCGGCGTCCGTaa > 1:703837/1‑238 (MQ=255) cacCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCgg > 1:357050/1‑216 (MQ=255) cacCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGAATTGCCCACCCGTTACCGGATATGAGTAGCGGCTTCCGTaac > 1:255173/1‑238 (MQ=255) ccTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGACTGGGCGCGAAT‑GAAAGAAAACA‑ACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAGGCAGCACGTCAGATGAAGGTGGCAGCGAACGAAAATCAATCCTGCATCGACTGTCATAAGGGTATTGCCCACCAGTTACCGAAAATGAGCAGAggacaccgaaagga > 2:323687/1‑227 (MQ=255) ccTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGCCTGTCATAAAGGAATTGCCCACCAGTTACCGGATATGAGTAGCGGCCTCCGTaacaa > 1:916655/1‑236 (MQ=255) ccTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGAATTGCCCCCCAGTTACCGGATATGATAAGCGGCTCCCGTAAGCa > 2:14760/1‑239 (MQ=255) ttCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGAATTGCCCCCCAGTTACCGGAAATGAGCAGCGGCTTCCGTAAAAAGTTCGCTGa > 2:58496/1‑239 (MQ=255) cGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGCTGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGCTGAAGGTGGCAGCGAACGATAATCAATCCTGCATCGACTGTCATAAAGGACTTGCCCACCAGTTACCGGATATGAGCAACGGCATCCGAAAGAAGTGCGAAGAGc > 2:52195/1‑239 (MQ=255) aaaccggcgGAAATTGCCGGGCGTAAATGGGCGCGAAT‑GAAAGAAAACA‑ACCCGGCAACCGGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTgcgcgc < 1:58496/233‑1 (MQ=255) gaacctgcccggggggaaaTGGGCACGAAG‑GAAAAAAAACA‑CCTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCt < 1:465372/221‑1 (MQ=255) aCTTGCCGAGCTGGAAGGGGCGCGAAT‑GAAAGAAACCA‑ACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAAt < 1:14760/238‑1 (MQ=255) cTTGCCGGGCGTGAAGGGGCGCGAAT‑GAAAGAAAACA‑ACTTGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGa < 1:75687/239‑1 (MQ=255) gCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCTTGAAGCAGCACGTCAGATGAAGGTgg > 1:858452/1‑116 (MQ=255) tttatgggggggaaaT‑AAAAGAAACCC‑CCTCGGAACCCCGCCGCTCCTGACATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGac < 2:154785/226‑1 (MQ=255) gaaaTGGTCGCAAAT‑CAGAAAAATCA‑CCTGGGCAACCGGCCGCTCCTGCCATAACTGCGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTTGTGaca < 1:344165/237‑1 (MQ=255) taaaggggggccatgaaaaaaaaaCA‑ACTCGGCAACCGCCCGCTCCTGCCATAACCACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGac < 2:916655/222‑1 (MQ=255) aTGGGCGCGAAT‑GAAAGAAAACA‑ACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAACAGGTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGACACGc > 2:640545/1‑239 (MQ=255) aTGGGCCAGAAT‑GAAAGAAAAAA‑AGCCGGCACCCTGCCTGCCCTGGCATAACTACGATGCGATGGATCAGGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGACACg < 1:555004/238‑1 (MQ=255) agggggcagaaggaagaaaaaCA‑ACCCGCCACCCTGCCGGTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTGCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGACACg < 1:723751/221‑1 (MQ=255) gggaggaaaT‑GAAAGAAAACC‑ACCTGGACACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGACACGCTg < 1:871632/232‑1 (MQ=255) ggaaaaaaaaaaaaaaAC‑ACGCGGAAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGACACGCTGTa < 2:413012/230‑1 (MQ=255) gcgAAT‑GAAAAAAAAAACACCGGGCAACTCGCCCACCCGGACATAACTACGATGAGATGGATGATGCAGAGCAACATACTGAAGCAGCAGGTCAGGTGAAGGTGGCAGCGAAAGATAATCAAACCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGACACGCTGTa < 2:407925/238‑1 (MQ=255) ccaaaT‑GAAAGAAAACA‑ACCCCTCACCCCCCCCCTCCTGCCAAAACTACGATGCAATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGACACGCTGTAc < 2:255173/235‑1 (MQ=255) aT‑GAAAACAAACA‑CCCCGGCAACCTGCAGCCCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGACACGCTGTACTCTa < 1:473529/238‑1 (MQ=255) | GTGTATGAAGAATATAAACAGTCGGTGCATTTCCAGAA‑CGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTCATATCCCGCCGGATA‑TTCCAGGCATGGTGAAGCGCAAACTGG‑AAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAATGGGCGCGAAT‑GAAAGAAAACA‑ACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGACACGCTGTACTCTA > NC_000913/1058252‑1058708 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |