Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,335,743 A→T 18.2% intergenic (+285/‑88) yciX → / → acnA uncharacterized protein/aconitate hydratase 1, aconitase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,335,7430AT18.2% 155.3 / 2.1 60intergenic (+285/‑88)yciX/acnAuncharacterized protein/aconitate hydratase 1, aconitase A
Reads supporting (aligned to +/- strand):  ref base A (17/31);  new base T (9/3);  total (26/34)
Fisher's exact test for biased strand distribution p-value = 2.14e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACACCGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGAA  >  NC_000913/1335508‑1335971
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                    
accccGCATCAATAACAGTTTGAGTTTTCTAAACAAAAAATAATAACCGGTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCa                                                                                                                                                                                                                                   <  2:20505/236‑1 (MQ=255)
  acCGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGTCTTGCCCCAAGGGTTCTCCTCTTTTATCAACTTTGGTTGTTTTCaaa                                                                                                                                                                                                                                 >  1:134078/1‑239 (MQ=255)
    cGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTGCTTTTCCACAAGGTTTTTCCCCTTTTTTCAATTTGGGTTTTTTTCcaa                                                                                                                                                                                                                                 >  1:318829/1‑237 (MQ=255)
          aaTAACAGCTTGGTTTTACTCAACAAAAAAAAATAACCGTTAAGGGGTTAGCCTATGATCAACACAAATTTGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTTCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTAcg                                                                                                                                                                                                                         <  1:795926/239‑1 (MQ=255)
             aacaattttgtttatcccaaacaaaaaTAATAACCGTTAAGGGGTTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGa                                                                                                                                                                                                                      <  2:329471/216‑1 (MQ=255)
              aCAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTTTCCCTTTGTTTTCCACCAAGTTTTTCCCCTTTTTTCAATTTGGGGTGTTTTCAAACCGTTACGCGaa                                                                                                                                                                                                                     >  1:96189/1‑238 (MQ=255)
                cgttttatttATCTCAAAAAAAAAAAATAACCGTTAAGGTTGTCGCCTAGGATCAACCAAAAGTTGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGGCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGt                                                                                                                                                                                                                   <  2:337426/231‑1 (MQ=255)
                  cTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCCTTTTACTTTCCCCCAAGGTTTTTCCCTTTTTTTCATTTTGGGTGTTTTTCaaaacttaagggtgtttt                                                                                                                                                                                                                  >  2:53594/1‑224 (MQ=255)
                      aGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTgtgt                                                                                                                                                                                                             <  1:257950/239‑1 (MQ=255)
                      aGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCCTTTGCTTTTCCACAAGGGTTCTCCTCTTTTTTCAATTTTGGGTGTTTTCAAAACGCTTAGCGATGTTTgggt                                                                                                                                                                                                             >  2:279033/1‑239 (MQ=255)
                         ttattcAACCAAAAATAATATCCTGTAAGGGTGTGGCCTTTGAGCAACAAAATTATTAATTAGTGGGCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTAt                                                                                                                                                                                                          <  2:880689/235‑1 (MQ=255)
                             taaaCAAAAAATAATCACCTTTAAGGGTTTAGCCTTTGATCAACCCAAATATGAATATTTGGTCCTGGATGGGCGCTTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCGCTGGATCATTACTCCCTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCttt                                                                                                                                                                                                      <  2:318829/237‑1 (MQ=255)
                                      aataatAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGGCCATTGCTTTGCCACAAGGTTTTTCCTCCTTTTTTCATTTGGGTTGTTTTTAAATTGTTACGGCAGGTTTTGTTTTTTTTaaaaaatta                                                                                                                                                                                             >  2:474293/1‑231 (MQ=255)
                                       aaaaatccctttAAGGGTTTTGCTTATGACACAACAAAATTTGAAATATTGGTCCCGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCAcc                                                                                                                                                                                            <  2:134078/229‑1 (MQ=255)
                                          ataaCCGTTAAGGGTTGAGCCTTGTTTCAACACAAATATGAAATTTTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTTGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTg                                                                                                                                                                                         <  2:784755/239‑1 (MQ=255)
                                                   aaGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTGCTTTTCCCCAAGGTTTTTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGCTGTTTGTGTTATCTTTAATATTTACCCTGAAGACAATc                                                                                                                                                                                >  1:195766/1‑239 (MQ=255)
                                                         gtAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCCTTTGCTTTGCCCCCAGGTTTTTCCTCTTTTATCAATTTTGGTTGTTTTCAAATCGGTACCCGCTGTTTGTGTTATCTTTAAAATTTACCCTTAAGAGAATAAAGGCt                                                                                                                                                                          >  1:751697/1‑239 (MQ=255)
                                                          tAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCCTTTGCTTTTCCCCAAGGTTTCTCCTCTTTTATTAATTTGGGGTGTTATCAAATCGTTACGCGATTTTTTTGTTTTCTTTAATATTCACCCTGAAGAAAATCAggggt                                                                                                                                                                          >  1:685693/1‑236 (MQ=255)
                                                                aTGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCCTTTTCTTTGCCCCAAGGGTTCTCCCCTTTTATCCATTTGGGTGGTTTTCAAATCGTTACCTGGTGTTTGTGTTTTTTTTAATTTTTACCCGTAAGAGAATAAAGGGTTCggca                                                                                                                                                                    >  2:878830/1‑235 (MQ=255)
                                                                  tgttCAACAAAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCGTTCGATGCTCTTCTGGGCCGAACTCCTCTGGAGCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAAcc                                                                                                                                                                  <  2:533055/236‑1 (MQ=255)
                                                                  taTCAACCCAAATTTGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGGCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAAccc                                                                                                                                                                 <  1:199051/238‑1 (MQ=255)
                                                                  gATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAAccc                                                                                                                                                                 <  1:279033/239‑1 (MQ=255)
                                                                   aacaaaaccaAATATGAAATTTTGGTCCTGGGTGGGCGCGTTTTCCCTGGCCAATGCCTTCCGGGCCGAACTCCTCTGGATCATTACTCACTTATCCGTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAAccc                                                                                                                                                                 <  2:96189/231‑1 (MQ=255)
                                                                        acacAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGCACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTTCCCACAAGTTTCTCCTCTTTTTTTAAATTTGGTTTTTATCAAATCGTTTCGCGATGTTTGTGTTATTATTTATATTCTACCTCAAGAGAATCAGAGCTTCGCAACCCCGt                                                                                                                                                              >  1:277628/1‑237 (MQ=255)
                                                                        acacAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTGCTTTGCCACAAGGGTTCTCCTCTTTTATCAATTTTGGTTGTTTTCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTTACCCTGAATAGAATCAGGGCTTTGCCACCCCGTCa                                                                                                                                                            >  1:114849/1‑239 (MQ=255)
                                                                          ccaAATTTGAAATTTTGGTCCTGGATGGGGGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCCCTGGATCATTACTCCCTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCAtt                                                                                                                                                          <  2:883358/238‑1 (MQ=255)
                                                                             aaTATGAAATTTTGGTCCTGGATGGGCGCGTTTTCTCTGGCGATGCGCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAag                                                                                                                                                       <  2:195766/239‑1 (MQ=255)
                                                                              atatGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTTTGTTTTTTTTAATATTTACCCTTAAGAGAATCAGGGCTTTCCAACCCTGTCATTAagg                                                                                                                                                      >  1:883358/1‑239 (MQ=255)
                                                                                attaaaTGTTGGGCTTGTGTGGGCTCTTTTTCTCTGTCGAGCCTCGTCTGGGACGAACTCCTCGGGATCATGACTCACTGATCCTGGCCCCCGCTGCGGCGGGGTTGTCATTTGCTTTTCCACAAGGTTTCTCCTCTTGTAGCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCGTGTCATTAaggag                                                                                                                                                    <  2:277628/236‑1 (MQ=255)
                                                                                 tGAAAGTTTGGGCCTGGATGGGCCCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGAGCCTTGACCCCGATGCGGCGGGGTTGTCATTTGCTGTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAagga                                                                                                                                                     <  2:203754/237‑1 (MQ=255)
                                                                                   aaaTATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTTGCTTGCCCCAAGGTTTTTCCCCTTTTTTCAATTTGGGTTGTTATCCAATCGTTACGCGATGTTTGTTTTATATTTAATATTCACCCTGAAGAGAATAAGGGCTTCGCCACCCTTTCATTAAtgagga                                                                                                                                                  >  1:203754/1‑238 (MQ=255)
                                                                                                 gATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAAAGAATCAGGGCTTCGTAACCCTGTACTTAAGGAGGAGCTATGTCGTCAAc                                                                                                                                    >  1:569234/1‑238 (MQ=255)
                                                                                                          gTTTTCTCTGGCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGa                                                                                                                                                                                        <  1:135811/177‑1 (MQ=255)
                                                                                                          gTTTTCCCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGaa                                                                                                                          <  1:608093/239‑1 (MQ=255)
                                                                                                                                    gCCGACCCCCTTTTGTTCCTTACTCCCTGATCCTTGACCCCGCTGGGGGGGGGGTGTCATTTGCTTTGCCACAAGGTTTCCCCTCTTTTATTAATTTTGGTGTTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGcc                                                                                                  <  2:722529/238‑1 (MQ=255)
                                                                                                                                          tgcctcGGGATTATTACTCACTGATCCTTGACCCCGCTGGGGCGGGGTTGGCATTTGCTTGGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTAACAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATa                                                                                           <  1:190545/237‑1 (MQ=255)
                                                                                                                                                    ttaTTACTCACTGATCCTTGACCCCGCTTGGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTTTCCTCTTTTATCAATTTTGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCa                                                                                 <  2:8698/237‑1 (MQ=255)
                                                                                                                                                    tCATTACGCACTGATCCTTGACCCCGGTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTAcc                                                                                  <  1:187995/238‑1 (MQ=255)
                                                                                                                                                    tCATTACCCACTGATCCTTGACCCCGGTGCGGCGGGGTTGTCATTTGATTTGCCACAAGGTTTCTACTCTTTTATCAATGTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTAcc                                                                                  <  1:801867/238‑1 (MQ=255)
                                                                                                                                                       ttACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGGTCTTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTTGGTTGTTATCAAATTGATACGCGATTTTTGTGTTATCTTTTATATTCACCCTCAAGAGGAACCGGGGTTTGCAACCCTTGCATTAAGGAGAGGCCTTATCGTCAACCCCCAGAGAAGCCAGGAAGGCACCATTGGCGGCCaaaaaaaaaaatatatca                                                                                >  2:190545/1‑229 (MQ=255)
                                                                                                                                                                       gACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCCACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGACTGCCg                                                                 >  1:23519/1‑236 (MQ=255)
                                                                                                                                                                         ccccGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTAAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTtgc                                                            <  1:474293/239‑1 (MQ=255)
                                                                                                                                                                           ccGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCAtt                                                                                                                                                          <  2:196007/143‑1 (MQ=255)
                                                                                                                                                                           ccGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAAGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCAACCTGAAGAGAATCAGGGCTTCGCAACCCTGTCAtt                                                                                                                                                          >  1:196007/1‑143 (MQ=255)
                                                                                                                                                                            ttttttaaagagtcggtgtaTTTTCTTGACCACTAGTTTTTCCCTTTTGTATCAAGTTTGGTTGTTTACAAATCGTGACGCGATCGTTGTGGTATCTTTAATATTCACCCTGAAGAGAAGCAGGCCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAACCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTTCAGCCTGCCGCTtgc                                                            <  2:235244/218‑1 (MQ=255)
                                                                                                                                                                            cGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTTTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGcc                                                                                                  <  2:154835/198‑1 (MQ=255)
                                                                                                                                                                            cGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGcc                                                                                                  >  1:154835/1‑198 (MQ=255)
                                                                                                                                                                                    cggGGTTTTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCa                                                  <  1:878830/238‑1 (MQ=255)
                                                                                                                                                                                           gTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTTGGCGa                                          >  1:89891/1‑239 (MQ=255)
                                                                                                                                                                                                 ttgctttgcCACAAGGTTTTTCCTTTTTTATCAATTTGGGTTGTTTTCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCa                                     <  2:114849/239‑1 (MQ=255)
                                                                                                                                                                                                        tgcCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCAACCTGACGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGCGGAGCTATGTCGTCACCCCTACGAGAAGCCAGTAAGGAGCCGTTGCAGGCCAACGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCAcctgtctcttctacaaat                                >  1:235244/1‑221 (MQ=255)
                                                                                                                                                                                                                 agagtcttttttttttCAATTTTGGTTGTTTTCAAATCGTTACGCGATGTTTGTGTTTTCTTTAAAATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGt                      <  2:413886/229‑1 (MQ=255)
                                                                                                                                                                                                                  tttCTCCTTTTTTATCAATTTGGGTTTTTATCAAATCGTTACGGGATGTTTGTTTTATCTTTAATATTCACCCTGAAGGGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTAGGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGt                      <  2:751697/238‑1 (MQ=255)
                                                                                                                                                                                                                    tctcCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCa                    >  1:672250/1‑238 (MQ=255)
                                                                                                                                                                                                                     ttcctcTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACt                  <  2:672250/238‑1 (MQ=255)
                                                                                                                                                                                                                           ttttATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAagg                                                                                                                                                      <  2:426594/101‑1 (MQ=255)
                                                                                                                                                                                                                           ttttATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAagg                                                                                                                                                      >  1:426594/1‑101 (MQ=255)
                                                                                                                                                                                                                            tttATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGCTAAAACTTACCACTACTACACCCTGCCGCTTGCGCCTAAATCACTGGACGATCACACCCGCACACCAAAACCACTCAAACtt           >  2:284081/1‑239 (MQ=255)
                                                                                                                                                                                                                                    tttGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTCTgactct   >  1:413886/1‑234 (MQ=255)
                                                                                                                                                                                                                                     ttGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGaa  >  1:27884/1‑239 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                    
ACACCGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGAA  >  NC_000913/1335508‑1335971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: