Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 71,877 A→G 18.0% E176G (GAG→GGG)  yabI → DedA family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091371,8770AG18.0% 120.9 / 3.7 56E176G (GAG→GGG) yabIDedA family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (9/35);  new base G (8/3);  total (17/39)
Fisher's exact test for biased strand distribution p-value = 1.86e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGG  >  NC_000913/71642‑72109
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                            
ctTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGGTATTCCTTCCGGAATGCCGAGCGGTGAggt                                                                                                                                                                                                                                           >  1:192362/1‑237 (MQ=255)
        aaCTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGGTCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCGGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTTGCGGGCGCTGCGCTCGATATTCCTGCCGGGATGCAGGGCGGTGAGGTTAAATGGt                                                                                                                                                                                                                                   >  1:915249/1‑239 (MQ=255)
        aaCTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGGTTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCCAAGCGGGGGGTTTAAAAGGt                                                                                                                                                                                                                                   >  1:814182/1‑239 (MQ=255)
          cTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGACTATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCCGGGATTCTGGCGGGCGGGGGGGCCGGATTTTCTGCCGGGATGCGAGGGGGGTGGGTTTAAAggggt                                                                                                                                                                                                                                  >  2:711018/1‑237 (MQ=255)
          cTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGGGCGGGGTTCGCATTTCCTTCCGGGATGCCAGGCGGTGGGGTTTAATGGtgt                                                                                                                                                                                                                                 >  1:116112/1‑237 (MQ=255)
                       tcatgaaccaagttttttCCCCAGTTTGGTGGGGCTTTTTTGTGGCCCGCCGCGTCCGCTGGGGACATGGGTGGGGGTAATGTTGGGTCTGCCGGTGGCTAAATTTATTACGCCGAATTTGATCGGCGGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAg                                                                                                                                                                                                                    <  2:346976/223‑1 (MQ=255)
                       gCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCCACGCGTCCGCTGGTGCCAATGGTGGCGGGAAAGCGGGATCGGCCGGTGGCTAAATTTATTACGCCGGATATTTTTGGCGGCCTGCTGTGGGCGCCGTTTTCGTTCCTGCCCGGGATTTTGGCGGGCGCGGGGATCCATTATCTTGCCGGGGAGGAGCGGGGCGAGTTTGAATTGTTGTTACTGGTCACAg                                                                                                                                                                                                                    >  2:560501/1‑239 (MQ=255)
                         aTCAACACAGCATGTTCCCCATTCTGGTCGGTTGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCg                                                                                                                                                                                                                  <  1:197183/239‑1 (MQ=255)
                         aTCAACACAGCATGTTCACCATGCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGGGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCg                                                                                                                                                                                                                  <  1:711018/239‑1 (MQ=255)
                            aacccggcgttttCCCCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAAGGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGgtg                                                                                                                                                                                                               <  1:907308/228‑1 (MQ=255)
                                  gCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGCATATTATCGCCTGCCTGCTTGGGCCGCCGTTTTACTTCCTCCCAGGGATTTTGGCGGGGGCGGGGATCGTGATTCCTGCCGGCGTTGAGAGGGGGGAGTTTAAATGGTGTGTCCTGGGAACACGGGGGtttttt                                                                                                                                                                                                         >  2:673102/1‑238 (MQ=255)
                                  gCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGCGCCGGCGATCGATATTCCTGCCGGAATGCAGAGCGAGGGGTTTAAATGGGTGCTGCTGGCAACACGGGGGGTTTtg                                                                                                                                                                                                         >  1:17002/1‑239 (MQ=255)
                                   cATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCCCGGCGATCGATATTCCTGCCGGAATGCAGGGCGGTGAGGTTGAATGGTTGGTGCTGGCAACAGGGGGGgtttttt                                                                                                                                                                                                        >  1:584822/1‑237 (MQ=255)
                                                   gtcggtcgTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGTCGGGAATGCTGGGTCTGCCGGCGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGGTTTACTTTCCGCCAGGGATTCTGGCGGGCGCGGGGATGGCATGTCCTGGCGGAAGGCGGAGGGGGGGGGTTTAATGGGTGCTGGTGGCAACGGGGGGGGTTTTGgggggcggggggggggg                                                                                                                                                                                        >  2:727129/1‑226 (MQ=255)
                                                   gtcggtcgTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGACTGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCCGCCAGGGATTCTGGCGGGCGCGGGGGTCGCGATTTCTGCCGGGATGCAGAGCGGGGGGGTTTAATGGGGGGTGGTGGCGACCGCGGGGttttttggggggggggggggggg                                                                                                                                                                                        >  2:847778/1‑221 (MQ=255)
                                                   gtcggtcgTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTTGATCTGCCGGTGGCTAAATTTATTACGCCGGATATTATCGGCCGCCAGCTGTGGTCGCCGGTTTACTTCCTTCCAGGGATTCTGGCGGGGGCGGGGATCGATATTTCTGCCGGAATTGAGAGCGGGGGGTTTAAAATGTTGCTGTTGCGGCCAGCGGTGTTTTTGTgggggggggggtgggt                                                                                                                                                                                        >  2:619397/1‑226 (MQ=255)
                                                      ggtcgTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGCTATTCCTGCCGGAATGCAGAGCGGGGGGGTTAAATGGTTGCTGCTGGCAACAGCGGGGTTTTTGTGGGTTGGGGgctggctggg                                                                                                                                                                                     >  1:281678/1‑237 (MQ=255)
                                                           ttttGTTGGCGCGTCGGTTCCGCTGTTGCCATGGGTGGGGGGAATGGGGGATTGGCGGGGGGTAAAATTTATTACGCAGAATATGATGGGCTGCCGGGTGGGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGc                                                                                                                                                                                <  2:192362/239‑1 (MQ=255)
                                                                    gccggggggTCCGGTGGTCGAAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCGGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATgg                                                                                                                                                                        <  1:220770/230‑1 (MQ=255)
                                                                     ccgccgGGTCGGCTGGTGCCAATGGGGGCGTGAATGCTGGATCTGCGGGTGGCTAAATTTATTACGCTGAATATGATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGgcg                                                                                                                                                                      <  1:762029/239‑1 (MQ=255)
                                                                     ccGGCGCGTCCGCTGGGGCCAAGGGGGGCGGTAATGTCGGATCTGCCGGTGGCTAAATTTATTACGCCGAATTTTATCGGCTGCCGGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGgcg                                                                                                                                                                      <  1:133705/239‑1 (MQ=255)
                                                                              ccGCGGGTGCCAATGGGGGCGGAAATGCTGTACCTGCCGGTGGCTAAATTTAGTACGCTGAATATTAGCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTa                                                                                                                                                             <  1:619397/238‑1 (MQ=255)
                                                                                   ggTGCCATTGGGGGGGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTCCGCCGAATATTATGGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGa                                                                                                                                                       <  1:902768/239‑1 (MQ=255)
                                                                                     ggcaaaggggggCGGGAATGCTGGATTTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCTTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGa                                                                                                                                                       <  1:810372/227‑1 (MQ=255)
                                                                                                   gggcttcTGGTTGTGGCGGTGCTTATATTTATGTCGCCGAATTTTAGCGGCTGCCTGCTGTGGCCGCCGTTTTCCGTCCGGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTc                                                                                                                                        <  2:915249/232‑1 (MQ=255)
                                                                                                                 gCCGGGGGCTAAATTTATTACGCCGAATATTAGTGGCTGCCGGCGGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCcgcg                                                                                                                         <  1:501880/239‑1 (MQ=255)
                                                                                                                           tatttttttgccccgattttttgcggcggCCTGCTGTGGCCGCCTTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTttgtt                                                                                                               <  2:821722/216‑1 (MQ=255)
                                                                                                                                        cGAATTTTCTCGTGTGCTTGTTGTTGCCGCCGTTTTACTTCCGGCCAGGGATTCTGGAGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCg                                                                                                 <  2:116112/239‑1 (MQ=255)
                                                                                                                                          aaTATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTaa                                                                                                                                                            <  1:428307/179‑1 (MQ=255)
                                                                                                                                              ttatCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTc                                                                                                                                        <  1:636541/195‑1 (MQ=255)
                                                                                                                                                gtCGGCTGCCTGTTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTc                                                                                         <  1:292877/238‑1 (MQ=255)
                                                                                                                                                       gcctgcTGTGGCCGCCGTTTTCCTTCCGGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCAt                                                                                   <  1:475756/238‑1 (MQ=255)
                                                                                                                                                        cctgcTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGGGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTTTTGCGGCGCGGGAAAACGACTGGCCGTTTGTGTCATTATTTTTTCCGCGGGCGGTTGTTTTGGCTGTCACCCGTGGTTTTTGCCCTCg                                                                                 >  1:483794/1‑239 (MQ=255)
                                                                                                                                                                    gccgTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGGTGGTGGCTGGCTGTGCTGGCGGTTTTGGGGCCGCGGGTGAAGGGACGGCCCGTTTGAGGATTTATTTTTCCCCCGGGGTTTGTTTGGGGTGCTCGCCTTTGTTTTTTGTTATCGGCGgggggggg                                                                     >  2:690553/1‑237 (MQ=255)
                                                                                                                                                                      tcttttTCTTTCTGGCAGGGTCTTTGGGGTGCGGGGGGATCTGGTTTCCTGCCGGAATGAAGGGCGGGTGTGTTTAATGGTTGCTGCTGGCACCAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCtg                                                                 <  2:814182/237‑1 (MQ=255)
                                                                                                                                                                                        ggATTCTGGCGGGCGGGGCGATCGATATTCCTGCCGGAATGCAGGGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCa                                                <  1:690487/238‑1 (MQ=255)
                                                                                                                                                                                         gATTCTGGCGGGCGCGGCGATCGATATTCCTGGCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGcc                                                 <  1:394374/236‑1 (MQ=255)
                                                                                                                                                                                             ggggggggcggggcgTTGGATTTCCCGGGCGGAATGCAGAGGGGTGAGTTTAAATGGTTGCGGCTGGCAACAGCGGTGTTGTTGGGGGTGGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGGTCGAGTCATTATGTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGtt                                          <  2:666934/234‑1 (MQ=255)
                                                                                                                                                                                                  gggcgCGGCGTTCGATATTCCTGCCGGAATTCAGAGGGGTGAGTTTAAATGGTTGCTGCTGGCAACAGGGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGc                                     <  2:248822/239‑1 (MQ=255)
                                                                                                                                                                                                  gggcgCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATg                                      <  1:298896/238‑1 (MQ=255)
                                                                                                                                                                                                    gcgcGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGcc                                    <  1:673102/238‑1 (MQ=255)
                                                                                                                                                                                                     cgcgGCGATCGATATTCCTGCCGGAATGCAGGGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCg                                   <  1:690553/238‑1 (MQ=255)
                                                                                                                                                                                                        cggggtctggtttCCTGTCCGGAATGGAGAGGTGTGAGTTTAAATGGGTGCTGCGGGCAACAGCGGTTTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGGGGTTATGGAGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGgtg                                <  2:847784/228‑1 (MQ=255)
                                                                                                                                                                                                              cgatATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATc                          <  1:510042/238‑1 (MQ=255)
                                                                                                                                                                                                                        cctggctgaatgggggggggTGGGTTTAAATGGTTGCTGCTGGCAACGGGGGTTGTTTTGGGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATAtttt                  <  2:584822/221‑1 (MQ=255)
                                                                                                                                                                                                                          ccGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTTTGGGGCCGCGGTAAAACGACTGGCCGGTTTGGTCCTTTTTTTTTCCCCGGGCGTTTTTTTTGGCTGGAGGCCTTGTTTTTTTCCCTCTGCGTGGTGGTGGCGGGTGGGGTTATTTGCCCCCCCGTGGTTCCGGGGGtatagagtatttttgtg               >  2:430375/1‑226 (MQ=255)
                                                                                                                                                                                                                            ggAATGCAGAGGGGTTGGTTTAAATGGTTGGTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGGTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTaa            <  1:460988/239‑1 (MQ=255)
                                                                                                                                                                                                                              aaTGCAGGGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTaaa           <  1:727129/238‑1 (MQ=255)
                                                                                                                                                                                                                              aaTGCAGAGCTGTGAGTTTAAATGGGTGCGGGTGGCAAGCGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTGTGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTaaa           <  1:127304/238‑1 (MQ=255)
                                                                                                                                                                                                                              aaTGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTaaa           <  1:352672/238‑1 (MQ=255)
                                                                                                                                                                                                                                  cAGTGCGTTGAGTTTAAATGGTTGCTGCTGGCAACAGTGGTGTTTTTGTGGGTTGGTGGTTGGGTGTGGTGGCGGTTCTGGCGCATCGGTAAAGCGAGTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGGCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGt      <  1:455359/239‑1 (MQ=255)
                                                                                                                                                                                                                                    agagCGGTGAGTTGAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTAGGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGt      <  1:560501/238‑1 (MQ=255)
                                                                                                                                                                                                                                      ggCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGGGGTGTTTTTGGGGGTTGGTGGCTGGCTGTGCTGGCGGTTAGGGCGGAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTg    <  1:76586/237‑1 (MQ=255)
                                                                                                                                                                                                                                      ggCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGGGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTg    <  1:170192/237‑1 (MQ=255)
                                                                                                                                                                                                                                      agCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGGGCAGGGGGGAAGGGGATGGCCGGTTGAGTCATTTTTTTTTCCCCGGGCGGTTGTTTTGGCGGAGGCCCGTGGTTTTTGGCATCGCCGCGGTGGGGGCTGGGGGGGTTTATTTGCCCCCCCTTGTTGCCGGTGTatatgcaattattgtgtgagagggggg    >  2:149648/1‑216 (MQ=255)
                                                                                                                                                                                                                                         gggTGGGTTTAAATGGTTGCTGCGGGCAACCGCGGTGTTTTTGGGGGTTGGTGGCTGGCTGGGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTAGTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTggg  <  1:275083/237‑1 (MQ=255)
                                                                                                                                                                                                                                              aGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTTTGgcg                                                                                                                                                                      >  1:783867/1‑73 (MQ=255)
                                                                                                                                                                                                                                              aGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGgcg                                                                                                                                                                      <  2:783867/73‑1 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                            
CTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGG  >  NC_000913/71642‑72109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: