Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,825,164	A→T	14.1%	G154G (GGT→GGA)	spy ←	periplasmic ATP‑independent protein refolding chaperone, stress‑induced

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,825,164	0	A	T	14.1%	150.8 / 3.0	76	G154G (GGT→GGA)	spy	periplasmic ATP‑independent protein refolding chaperone, stress‑induced
Reads supporting (aligned to +/- strand):  ref base A (37/25);  new base T (8/4);  total (46/30)
Fisher's exact test for biased strand distribution p-value = 7.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAA‑GTTTTTTGGCTGAAAGATTATTCAG‑CAGTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCAT  >  NC_000913/1824933‑1825400                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGTAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGCCATTA‑GTTTTTTTTGTGAGAGACTAATTAG‑CAGGT‑GTCA‑GACATTTAA‑Cttttg                                                                                                                                                                                                                                                >  2:794121/1‑238 (MQ=255) tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCCAGGTAGTGGACAAGACCGGCGGCTTTAG‑GTGTTTTTGCGGAAAGAGTATTTGC‑GCGTT‑G‑CA‑GGGATTTTA‑CCtttt                                                                                                                                                                                                                                                >  1:552737/1‑238 (MQ=255) tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAA‑GGTTTTTGGGTGAAAATATATTCAA‑CGGGT‑T‑CA‑GGCATTTTT‑ACtttt                                                                                                                                                                                                                                                >  1:791375/1‑238 (MQ=255) tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAA‑ATTTTTTTGGTTAAAGATTATTCAG‑CAGTT‑G‑CA‑GGGATTTTA‑CCtttt                                                                                                                                                                                                                                                >  1:478023/1‑238 (MQ=255)      aCTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGGCTTAA‑GTTTTTTGGCTGAAAGATTATTCAG‑CAGTT‑G‑CA‑GGCATTTTA‑CCTTTTTcc                                                                                                                                                                                                                                             >  1:910338/1‑236 (MQ=255)        tCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAA‑GGTTTTTTGCTGAAAGATTATTCAG‑CAGTT‑G‑CA‑GGCAttttt‑cctttttcccccgg                                                                                                                                                                                                                                        >  1:142389/1‑234 (MQ=255)        tCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAA‑ATTTTTTGGCTGAAAGATTATTCAGACAG‑T‑G‑CA‑GGCATTTTA‑CCtatctcttttac                                                                                                                                                                                                                                        >  1:595082/1‑228 (MQ=255)          gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCCAGGTAGTGGACAAGACCGGCGGTCTTAC‑GTTTTTTTGCGATAAGAATATTTAG‑CAGGT‑G‑CC‑AGCATTTTACCCTTTTCCCGcggtc                                                                                                                                                                                                                                       >  1:73472/1‑235 (MQ=255)          gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAA‑GTTTTTTGGCTGGAAGATTATTTAG‑CAGGT‑G‑CA‑GGCATTTTA‑CCTTTTTGCGCTgg                                                                                                                                                                                                                                        >  1:637867/1‑237 (MQ=255)            tCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGGGGACAAGACCGGCGGCCTTAA‑GTTTTTTTGCTGAAAGATTATTCCG‑CAGTG‑T‑GA‑GGCCATTTA‑CCTTTTGCCGcctggcgt                                                                                                                                                                                                                                    >  1:878248/1‑232 (MQ=255)                                tGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTC‑CTTTTTTTGCTGAAAGTTTATTCAC‑CCGTTCG‑CG‑GGC‑GTTTC‑CCTTTTTCCGCTTGG‑CGttttttctaccacctttcaaa                                                                                                                                                                                                               >  2:285902/1‑220 (MQ=255)                                   tATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAA‑GTTTTTTTGCTGAAAGATTATTCAG‑CCAGT‑G‑CA‑GGCATTTTT‑CCTTTTTCCGCTGGA‑CGTTCTT‑TCA‑GAACCTTTTCCAAAta                                                                                                                                                                                                          >  1:185017/1‑238 (MQ=255)                                   tATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAA‑GTTTTTTGGCTGAAAGATTATTCAG‑CAGTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTT‑TCA‑CAAGCTTCTCAAAAtt                                                                                                                                                                                                          >  1:323026/1‑239 (MQ=255)                                                     ggATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGGTTTTTTGGC‑GAAAGATTTTTTAT‑CAATT‑G‑CAGGGCATTTAA‑CC‑TTTGCCGCTGGA‑CGGTTTG‑TCG‑AACGCTTCTCCAAATTAGCCTTAAATTCGttttt                                                                                                                                                                                        >  1:429897/1‑239 (MQ=255)                                                       aTGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAA‑GGTTTTTTGCTGAAAGGTTATTCAG‑CAGTT‑G‑CC‑AGCCTTTTA‑CCTTTTTCCCCTGGG‑CGTTCTT‑TCA‑GACGCTTTTCAAAATTAACATTAAATTTCttttttt                                                                                                                                                                                      >  1:586762/1‑237 (MQ=255)                                                                    aCTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCCTAA‑GTTTTTTTGCTGAAAGATTATTCAG‑GAGTT‑G‑CA‑GGCCTTTTA‑CCTTTTTCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAACTTATCATTTAATTGCTTTTTCTTTT‑CCGGGGTCAg                                                                                                                                                                        >  1:769892/1‑239 (MQ=255)                                                                     cTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAA‑GTTTTTTTGCTGAAAAATTATTTAG‑CAGTT‑T‑CA‑GGCATTTTT‑CCTTTTTCCGCTTGG‑AGGTCTG‑TCA‑AACGCTTTTCCAAATTAACATTTAATTGCTTTTTTTTTT‑TCgggggcggg                                                                                                                                                                       >  2:778538/1‑232 (MQ=255)                                                                                 aggTTAGGGGTAATCAGGTTTTTTTTACAATTGTT‑GGAAAAGAAAAAAGAAA‑C‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAA‑GTTTTTTGGCTGAAAGATTATTCAG‑CAGTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAAtt                                                                                                                                                         <  1:672657/237‑1 (MQ=255)                                                                                      aggggtgATCAGGTTTTTTTAACAGTTTTT‑GGAAAAGAAAAAAAAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAA‑GTTTTTTGGCTGAAAGATTATTCAG‑CAGTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGtt                                                                                                                                                    <  1:286089/236‑1 (MQ=255)                                                                                       tttggtgactgtttttttttATAGATGTT‑TAAAAAAAGAAAAAAAACG‑CCGCCGATCCTGTCATCCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAA‑GTTTTTTGGCTGAAAGATTATTCAG‑CAGTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGtt                                                                                                                                                    <  2:791375/226‑1 (MQ=255)                                                                                       gtggtgATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTA‑ATTTTTTTGCTGAAAAATTATTCTG‑CCCAT‑G‑CA‑TGCATTTTT‑CCTTTTGCCGCTGGACCTTTTTG‑TCA‑CAC‑CTTCTCCAAATTAACATTTAATTGCTTTTTTTTTT‑CCGGCGCCCAGAGT‑TTG‑TAAAAttttttt                                                                                                                                                   >  2:677444/1‑238 (MQ=255)                                                                                        tggtgATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAA‑GTTTTTTGGCTGAAAGATTATTCAG‑CAGTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTTTG‑TCT‑GCCGCTTCTCAAAATTAGCATTAAATTGCTTTTTTTTTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGt                                                                                                                                                     >  1:557864/1‑236 (MQ=255)                                                                                             aTCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTA‑GTTTTTTGGCTGAAAGCTTATTCCG‑CAGTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGTG‑CGTTCTG‑TCA‑GACGCCTCTCACAATAAGCATTAAATTTGTTTTTCTTTT‑CCGGGGTCAGGCTG‑TTG‑TTAATTTTGTTCTGGGtt                                                                                                                                             >  1:919049/1‑239 (MQ=255)                                                                                             aTCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAA‑GTTTTTTGGCTGAAAAGTTATTTAG‑CCGTT‑G‑CA‑GGCATTTTA‑CCTTTTTCCCCTGGA‑CGGTCTG‑TCC‑GACGCTTCTCAAAATTATCATTTAATTGCTTTTTTTTTT‑CCGGCGTCCGGGTGTTTG‑GAATTTTTTTTTTGGGt                                                                                                                                              >  1:211242/1‑239 (MQ=255)                                                                                             aTCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAA‑GGTTTTTTGCTTGAAAATTATTCAG‑CAGTT‑T‑CA‑GGCCTTTTT‑CCCTTTTCCGCTGGG‑CGTTCTGTTCA‑G‑CGCTTTTCCAAATTAACATTTAATTGCTTTTTTTTTTCCCGGCGGCGGGTTG‑TGG‑TAAATTTTTTTTTggtt                                                                                                                                              >  2:858070/1‑237 (MQ=255)                                                                                               taGGTTTTTTTTCAAACTGTTGTGAAAAAAAAAAAAAAA‑C‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAA‑GTTTTTTGGCTGAAAGATTATTCAG‑CAGTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGttt                                                                                                                                            <  1:692184/238‑1 (MQ=255)                                                                                                         ttAAAAATGTT‑TGAAAAAAAAAAAAAAA‑ACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAA‑GTTTTTTGGCTGAAAGATTATTCAG‑CAGTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTgcgc                                                                                                                                  <  1:568952/239‑1 (MQ=255)                                                                                                          caaaagtgtggggagaaaaaaaAAAAAA‑C‑CCGCCGATCCGTTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAA‑GTTTTTTGGCTGAAAGATTATTCAG‑CAGTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGcc                                                                                                                                 <  1:677444/227‑1 (MQ=255)                                                                                                          aactgtggTT‑TGAAAAAAAAAAAAAAC‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAA‑GTTTTTTGGCTGAAAGATTATTCAG‑CAGTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTgcgc                                                                                                                                  <  1:615513/230‑1 (MQ=255)                                                                                                            cAACTGTT‑TGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAA‑GTTTTTTGGCTGAAAGATTATTCAG‑CAGGT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTT‑TCA‑AACGCTTCTCAAAATTACAATTTAATTGCTTTTTTTGTT‑CCGGCGCCAGGATG‑TTG‑TAAAATTTGTTCTGGGTTTCCAttttggccacc                                                                                                                              >  1:909466/1‑229 (MQ=255)                                                                                                                 tttttagaaagaaaaAAGAAA‑T‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAA‑GTTTTTTGGCTGAAAGATTATTCAG‑CAGTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGt                                                                                                                          <  1:352080/231‑1 (MQ=255)                                                                                                                  tt‑tGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAA‑GTTTTTTGGCTGAAAGATTATTAAG‑CAGAT‑G‑CA‑GGGATTTTA‑CCTTTTTCCTCTGGA‑CGTTCTG‑TCA‑GAAGCTTCTCTAAATTAGCATTATATTTCTTTTTTTGTT‑TCGGGGTCAGGA‑G‑TTGTTTAATTTTTTTTTTGGtttttctatttccccgccagttt                                                                                                                        >  2:787195/1‑219 (MQ=255)                                                                                                                        gaagagaaAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAA‑GTTTTTTTGCTGGAAGATTATTTAG‑CAGTT‑T‑CA‑GGGATTTTT‑CCTTTTTCCGCTGGG‑GGTTCTT‑TTATGAC‑CCTCTCCAAATTTACATTAAATTGCTTTTTTTTTT‑TCGGGCGCACGAGA‑GTG‑TAAATTTTTTTTTGGGTTTTCaattggggccacagttttggttt                                                                                                                   >  2:433052/1‑217 (MQ=255)                                                                                                                           gagaaAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTA‑GTTTTTTTGCTGAAAGATTATTCAG‑CCGTT‑G‑TC‑GGCATTTTA‑CCTTTTGCCCCTGGA‑AGGTCTG‑GCC‑AACGCCTCTCCAAATTAGCCTTAAATTGATTTTTTTTTT‑TCCGCCGCCGGCTT‑GTG‑TAAATTTTGTTTTGGGGTTTCATTTGGGCCGACATTTTaagtgactc                                                                                                                >  2:615513/1‑231 (MQ=255)                                                                                                                            tgaaaagaaa‑a‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAA‑GATTTTTGGCTGAAAGATTATTCAG‑CAGTT‑G‑CA‑AGCCTTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCC‑GAAGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTTTT‑CCGGCGTCAGGGTG‑GTG‑TAAATTTTGTTCTTGGTTTCCATTTGCGCCCGCCAGCTAGCttttc                                                                                                                 >  1:17515/2‑235 (MQ=255)                                                                                                                                      aacccgccgATCCTGTCCACCGCATTACGGCAAGGTAGTGGACAAGACCGGCGGTCTTAA‑GTTTTTTGGCTGAAAGATTATTCAG‑CAGTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGttctt‑c                                                                                                      <  1:858070/236‑1 (MQ=255)                                                                                                                                       a‑CCGCCGATCCTGTCCATCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAA‑GTTTTTTGGCTGAAAGATTATTCAG‑CAGTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCa                                                                                                    <  1:794121/239‑1 (MQ=255)                                                                                                                                                   tGTCCACCGCATTTCTGCAAGGTTGTGGACAAGGCCGGCGGTCTTAA‑GTTTTTTGGCTGAAAGATTATTCAG‑CAGTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATc                                                                                         <  1:778538/239‑1 (MQ=255)                                                                                                                                                    gTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAA‑GTTTTTTGGCTGAAAGATTATTCAG‑CAGTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTTTT‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTTTT‑TCGGAGTCAGGATG‑TTG‑TAAATTTTTTTTTTGGGTTTCATGTTCGCCAGCATGTTAGCTTTTGCCTGTTCTT‑CCCTTTTTTCGa                                                                                           >  1:128179/1‑236 (MQ=255)                                                                                                                                                         ccGCATTACTGGAAGGTAGGGGACAAGACCGGCGGTCTTAA‑GTTTTTTGGCTGAAAGATTATTCAG‑CAGTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCt                                                                                  <  1:285902/239‑1 (MQ=255)                                                                                                                                                                       ggTAGTGGACAAGACCGGCGGTCTTAA‑GTTTTTTGGCTGAAAGATTATTTAG‑GAGTT‑G‑CA‑GGCATTTTA‑CCTTTTTCCGCTGGG‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCCTTAAATTTCTTTTTCTGTT‑TCCGGGGCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTTCATGTGCGCCAGCCTGTTAGCTTTTTGCTTTTTTT‑TCATTTTTGCGATC‑TGGGGTTTCCGTTTTACttt                                                                    >  2:92072/1‑239 (MQ=255)                                                                                                                                                                       ggTAGTGGACAAGACCGGCGGTCTTAA‑GTTTTTTGGCTGAAAGATTATTCAG‑CAGTT‑T‑CA‑AGCATTTTT‑CCTTTTTACGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCCAAATTAGCATTAAATTGCTTTTTTTGTT‑TCGGCGTCGGGATG‑TTG‑TAAATTTTTTTCTGGGGTTCCTTGTGCGCCATCATGTTAGCTTTTCGCTGTTCTTCCCATTTTTG‑GTTC‑TGCCGTTCAGCTTTTTCttt                                                                    >  1:133897/1‑239 (MQ=255)                                                                                                                                                                         tAGTGGACAAGACCGGCGGTCTTAA‑GTTTTTTGGCTGAAAGATTATTCAG‑CAGTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCg                                                                                            <  1:483229/214‑1 (MQ=255)                                                                                                                                                                          ggttgACAAAACCCGGGGTTTTAA‑GTGTTTTGGCTGAAAGATTATTCCG‑CAGTT‑G‑CA‑GGCATTTTT‑CCTTTTGCCTCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTGCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTGCTTTATc                                                                 <  2:31759/235‑1 (MQ=255)                                                                                                                                                                           gTGGACAAGACCGGCGGTCTTAA‑GTTTTTTGGCTGAAAGATTATTCAG‑CAGTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCCAAATTAGCATTTAATTGCTTTTTCTGTT‑CCGGCGTCCGGGTG‑TTT‑GAAAATTTGTTCTGGGGTTTCATTTTGCCCAGCATGTTTACTTTTCGCTGTTTTT‑CCATTTTTTGGGTC‑TTCGCTTTCGCTTTTTCTTTATCg                                                                >  1:914362/1‑239 (MQ=255)                                                                                                                                                                              gACAAGACCGGCGGTCTTAA‑GTTTTTTTGCTGAAAGATTATTTAG‑AAGTT‑G‑CA‑GGGATTTTT‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGTTTTTTTTTTT‑CCGGCGGCCGGGTG‑TTG‑TAAATTTTTTTCTGGGTTTTCATGTGCGCCACCATGTTAGCTTTGCGCTTTTCTT‑CCCTTTTTTCTCTC‑TTCGCTTTCGCTTTTTCTTTTTCGAAg                                                             >  2:177908/1‑239 (MQ=255)                                                                                                                                                                              gACAAGACCGGCGGTCTTAA‑GTTTTTTGGCTGAAAGATTATTCAG‑CAGTT‑G‑CA‑GGCATTTTA‑ACTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GAAGCTTCTCAAAATTAGCATTAAATTTCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑GAAATTTTTTTCTGGGTTTCCATTTGCGCCAGCCGTTTTGTTTTTGGCGGTTTTT‑CCATTTTTGCGATC‑TTGGCTTCGGCTTTTACTTTATctgag                                                             >  1:646786/1‑235 (MQ=255)                                                                                                                                                                              gACAAGACCGGCGGTCTTAA‑GATTTTTTGCTGAAAAATTATTCAG‑CAGTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCTCTTGA‑CGTTCTG‑TCA‑AACGCTTCTCCAAATTAACATTAAAATGCTTTTTCTTGT‑ACGGCGGCATGTTG‑TTG‑TAAAATTTGATCTTGGTTTCCATGTGGGCCAGCATGTTAACTTTGCGCATTTCTT‑CCATTTTTTCGATCATGC‑CTTCAACTTTTACTTTTTCGaaa                                                             >  1:647470/1‑238 (MQ=255)                                                                                                                                                                                 aaGACCTGCGGTTTTAA‑GTTTTTTGGCTGAAAGATTATTCAG‑CAGTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGt                                                           <  2:637867/238‑1 (MQ=255)                                                                                                                                                                                  agacccggtgttttAT‑GTTTTTTGGTGTAAAGATTTTGCAG‑CCGTT‑A‑CA‑GGCATTTTT‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTTAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTAt                                                         <  2:133897/227‑1 (MQ=255)                                                                                                                                                                                   gACCGGCGGTCTTTA‑GTTTTTTGGCTGAAAGATTATTCAG‑CAGTT‑G‑CA‑GGGATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGCTG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTTCGCCAGCATGTTAGCTTTGTCCGTTTTTT‑CCATTTTTGGGGTC‑TGCGCTTTAGCGTTTTCTTTTTTGAAGGTAt                                                         >  1:889933/1‑238 (MQ=255)                                                                                                                                                                                   gACCGGCGGTCTTAA‑GTTTTTTGGCTGAAAGATTATTCAG‑CAGTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCCAAATTAACATTTAATTGCTTTTTCTGTT‑TCCGCGTCCGGATG‑TTG‑TAAATTTTTTTTTGGGGTTTCCTTTGCGGCCGCCTGTTAGCGTTTCGCTGTTTTT‑TCCTTTTTTTGGTC‑TGCCCTTTACGTTTTACTTTTTCGGAgggaac                                                        >  2:225099/1‑235 (MQ=255)                                                                                                                                                                                   gACCGGCGGTCGTAA‑GTTTTTTGGCTGAAAGATTATTCAG‑CAGAT‑T‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTT‑TCA‑GACGGTTCTCAAAATTAACATTAAATTGCTTTTTCTGTT‑CCGCCGTCCGGGTG‑TTG‑TAAATTTTGTTTTGGGTTTTCATTGTCGCCCGCCAGTTGGCTTTTCGCCGTTCTT‑CCCTTTTTTCGCTT‑TGGGCTTCCGCTTTTTCTTTATTGCAGGTAtt                                                        >  2:836289/1‑238 (MQ=255)                                                                                                                                                                                      cggctgTTTTAT‑TTTTTTTGGGTGAAAGATTATGCAG‑CAGTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCt                                                     <  2:552737/239‑1 (MQ=255)                                                                                                                                                                                            ttttta‑GTTTTTTGGCTGAAAGATTATTCAG‑CAGTT‑G‑CC‑GGCATTTTT‑CCTTTTGCCGCTGGA‑CCGTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCaa                                                <  2:66474/233‑1 (MQ=255)                                                                                                                                                                                            ttttAA‑GTTTTTTGGTTGAAAGGTTTTTCAG‑CAGTT‑G‑CA‑GGAATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCaa                                                <  2:323026/236‑1 (MQ=255)                                                                                                                                                                                            ttctAA‑GTTTTTTGGGTTAAAGGTTTTTCAG‑CAGTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCaa                                                <  1:891995/235‑1 (MQ=255)                                                                                                                                                                                            tCTTTA‑GTTTTTTGGGTGAAAGATTATTCAG‑CAGTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCaa                                                <  2:586762/238‑1 (MQ=255)                                                                                                                                                                                            tCTTAA‑GTTTTTTGGCTGAAAGATTATTCAG‑CAGTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCaa                                                <  1:187490/238‑1 (MQ=255)                                                                                                                                                                                             tttAA‑TTTTTTGGGCTGAAAGTTTTTTCAG‑CAGTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                              <  2:185017/238‑1 (MQ=255)                                                                                                                                                                                               tta‑GTTTTTTGGCTGTAAGTTTTTTCAG‑CATTT‑G‑CA‑GGCATTTTT‑CCTTTTGCCGGTTGA‑CGTTCTG‑TCA‑GACGCGTCGTAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatgat                                            <  2:424309/237‑1 (MQ=255)                                                                                                                                                                                                       tttGGCTGAAAGATTATTCAG‑CAGTT‑G‑CA‑GGCCTTTTA‑CATTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTCAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTTCGCTGTTCTT‑CCATTTTTGCGATC‑TTCGCTTCAGCTTTTACTTTATCGAAGTGAACGCTAGCAATGATGTCCTGc                                     >  2:485277/1‑239 (MQ=255)                                                                                                                                                                                                       tttGGCTGAAAGATTATTCAG‑CAGTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCAt                                       >  1:449612/1‑237 (MQ=255)                                                                                                                                                                                                       tttGGCTGAAAGATTATTCAG‑CAGTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGc                                     >  2:319014/1‑239 (MQ=255)                                                                                                                                                                                                       tttGGCTGAAAGATTATTCAG‑CAGTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCCCTGTTCTT‑CCATTTTTGCGGTC‑TGCGCTTCAGCTTTTACTTTATGGAAGGTATCGCTGGCAATGATGGCATga                                     >  2:878432/1‑238 (MQ=255)                                                                                                                                                                                                              gAAAGATTATTCAG‑TAGTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GAAGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATGTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTgcgc                              <  1:906285/239‑1 (MQ=255)                                                                                                                                                                                                              gAAAGATTATTCAG‑CAGTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAAtt                                                                                                                                                         <  1:124531/118‑1 (MQ=255)                                                                                                                                                                                                              gAAAGATTATTCAG‑CAGTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAAtt                                                                                                                                                         >  2:124531/1‑118 (MQ=255)                                                                                                                                                                                                               taaagtttgtgtcgctttt‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCC‑GAGGCTTCCTAAAATTGGCAGTAAATTGCTTGTTCCGTT‑CCGGCGTCAGGATG‑GTG‑GAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGGTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGTTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTgcgc                              <  2:73472/222‑1 (MQ=255)                                                                                                                                                                                                                         cag‑cagTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTcc                   >  2:231589/1‑239 (MQ=255)                                                                                                                                                                                                                         cag‑cagTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGGCATGCATTGCGCGGCGtttttca                   >  2:129119/1‑238 (MQ=255)                                                                                                                                                                                                                                 t‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAgcggcg            <  1:92072/239‑1 (MQ=255)                                                                                                                                                                                                                                   g‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGTTTTTTCTGTT‑CCGGCGACAGGATG‑ATG‑TAAATTTTGTTCTGGGGTTCCATGTGCTCCAGCATTTTAGCTTTACGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGTAGGTATCGCTGGCAATGTTATCATCCAtctttctcttatacacctctccgt            >  1:505563/1‑215 (MQ=255)                                                                                                                                                                                                                                   g‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGGGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGGTC‑TGCGCGTCAGCTTTTACTTTATCGAAGGATTCGCTGGCAATGATGGCATGCCTTACGGGGCGTTCTTCCAGcggcgg           >  2:520840/1‑239 (MQ=255)                                                                                                                                                                                                                                    tgttggCTTTTTG‑CCTTTGGCCGTGGGA‑GGTTTTG‑TCA‑GGCGGTGCTCAAAAATAGCATTAAAGTGATTTTTCTTTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTGGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTTCGCTGTTCTT‑CCAGTTTTGCGATC‑TGCGCTTCAGCTTTTTCTTTATCGAAGGTATCGCTGGCAATGATGTCATTCATTGCGCGGCGTTCTCTCAgctgag            <  2:17515/235‑5 (MQ=255)                                                                                                                                                                                                                                          cATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCACGCGGCGGCCGtt      >  1:714361/1‑239 (MQ=255)                                                                                                                                                                                                                                              ttA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCAt  <  1:319014/239‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      TCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAA‑GTTTTTTGGCTGAAAGATTATTCAG‑CAGTT‑G‑CA‑GGCATTTTA‑CCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCAT  >  NC_000913/1824933‑1825400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑