Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,825,226 G→T 15.2% Q134K (CAG→AAG)  spy ← periplasmic ATP‑independent protein refolding chaperone, stress‑induced

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,2260GT15.2% 225.9 / 4.1 90Q134K (CAG→AAG) spyperiplasmic ATP‑independent protein refolding chaperone, stress‑induced
Reads supporting (aligned to +/- strand):  ref base G (29/46);  new base T (13/2);  total (42/48)
Fisher's exact test for biased strand distribution p-value = 1.15e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGG  >  NC_000913/1825000‑1825464
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   
aCTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCCTAAGTTTTTTTGCTGAAAGATTATTCAGGAGTTGCAGGCCTTTTACCTTTTTCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAACTTATCATTTAATTGCTTTTTCTTTT‑CCGGGGTCAg                                                                                                                                                                                                                                        >  1:769892/1‑239 (MQ=255)
 cTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAAATTATTTAGCAGTTTCAGGCATTTTTCCTTTTTCCGCTTGG‑AGGTCTG‑TCA‑AACGCTTTTCCAAATTAACATTTAATTGCTTTTTTTTTT‑TCgggggcggg                                                                                                                                                                                                                                       >  2:778538/1‑232 (MQ=255)
             aggTTAGGGGTAATCAGGTTTTTTTTACAATTGTT‑GGAAAAGAAAAAAGAAA‑C‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAAtt                                                                                                                                                                                                                         <  1:672657/237‑1 (MQ=255)
                  aggggtgATCAGGTTTTTTTAACAGTTTTT‑GGAAAAGAAAAAAAAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGtt                                                                                                                                                                                                                    <  1:286089/236‑1 (MQ=255)
                   tttggtgactgtttttttttATAGATGTT‑TAAAAAAAGAAAAAAAACG‑CCGCCGATCCTGTCATCCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGtt                                                                                                                                                                                                                    <  2:791375/226‑1 (MQ=255)
                   gtggtgATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAATTTTTTTGCTGAAAAATTATTCTGCCCATGCATGCATTTTTCCTTTTGCCGCTGGACCTTTTTG‑TCA‑CAC‑CTTCTCCAAATTAACATTTAATTGCTTTTTTTTTT‑CCGGCGCCCAGAGT‑TTG‑TAAAAttttttt                                                                                                                                                                                                                   >  2:677444/1‑238 (MQ=255)
                    tggtgATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTTTG‑TCT‑GCCGCTTCTCAAAATTAGCATTAAATTGCTTTTTTTTTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGt                                                                                                                                                                                                                     >  1:557864/1‑236 (MQ=255)
                         aTCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGTTTTTTGGCTGAAAGCTTATTCCGCAGTTGCAGGCATTTTACCTTTTGCCGCTGTG‑CGTTCTG‑TCA‑GACGCCTCTCACAATAAGCATTAAATTTGTTTTTCTTTT‑CCGGGGTCAGGCTG‑TTG‑TTAATTTTGTTCTGGGtt                                                                                                                                                                                                             >  1:919049/1‑239 (MQ=255)
                         aTCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAAGTTATTTAGCCGTTGCAGGCATTTTACCTTTTTCCCCTGGA‑CGGTCTG‑TCC‑GACGCTTCTCAAAATTATCATTTAATTGCTTTTTTTTTT‑CCGGCGTCCGGGTGTTTG‑GAATTTTTTTTTTGGGt                                                                                                                                                                                                              >  1:211242/1‑239 (MQ=255)
                         aTCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGGTTTTTTGCTTGAAAATTATTCAGCAGTTTCAGGCCTTTTTCCCTTTTCCGCTGGG‑CGTTCTGTTCA‑G‑CGCTTTTCCAAATTAACATTTAATTGCTTTTTTTTTTCCCGGCGGCGGGTTG‑TGG‑TAAATTTTTTTTTggtt                                                                                                                                                                                                              >  2:858070/1‑237 (MQ=255)
                           taGGTTTTTTTTCAAACTGTTGTGAAAAAAAAAAAAAAA‑C‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGttt                                                                                                                                                                                                            <  1:692184/238‑1 (MQ=255)
                                     ttAAAAATGTT‑TGAAAAAAAAAAAAAAA‑ACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTgcgc                                                                                                                                                                                                  <  1:568952/239‑1 (MQ=255)
                                      caaaagtgtggggagaaaaaaaAAAAAA‑C‑CCGCCGATCCGTTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGcc                                                                                                                                                                                                 <  1:677444/227‑1 (MQ=255)
                                      aactgtggTT‑TGAAAAAAAAAAAAAAC‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTgcgc                                                                                                                                                                                                  <  1:615513/230‑1 (MQ=255)
                                        cAACTGTT‑TGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGGTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTT‑TCA‑AACGCTTCTCAAAATTACAATTTAATTGCTTTTTTTGTT‑CCGGCGCCAGGATG‑TTG‑TAAAATTTGTTCTGGGTTTCCAttttggccacc                                                                                                                                                                                              >  1:909466/1‑229 (MQ=255)
                                             tttttagaaagaaaaAAGAAA‑T‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGt                                                                                                                                                                                          <  1:352080/231‑1 (MQ=255)
                                              tt‑tGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTAAGCAGATGCAGGGATTTTACCTTTTTCCTCTGGA‑CGTTCTG‑TCA‑GAAGCTTCTCTAAATTAGCATTATATTTCTTTTTTTGTT‑TCGGGGTCAGGA‑G‑TTGTTTAATTTTTTTTTTGGtttttctatttccccgccagttt                                                                                                                                                                                        >  2:787195/1‑219 (MQ=255)
                                                    gaagagaaAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGGAAGATTATTTAGCAGTTTCAGGGATTTTTCCTTTTTCCGCTGGG‑GGTTCTT‑TTATGAC‑CCTCTCCAAATTTACATTAAATTGCTTTTTTTTTT‑TCGGGCGCACGAGA‑GTG‑TAAATTTTTTTTTGGGTTTTCaattggggccacagttttggttt                                                                                                                                                                                   >  2:433052/1‑217 (MQ=255)
                                                       gagaaAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGTTTTTTTGCTGAAAGATTATTCAGCCGTTGTCGGCATTTTACCTTTTGCCCCTGGA‑AGGTCTG‑GCC‑AACGCCTCTCCAAATTAGCCTTAAATTGATTTTTTTTTT‑TCCGCCGCCGGCTT‑GTG‑TAAATTTTGTTTTGGGGTTTCATTTGGGCCGACATTTTaagtgactc                                                                                                                                                                                >  2:615513/1‑231 (MQ=255)
                                                        tgaaaagaaa‑a‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGATTTTTGGCTGAAAGATTATTCAGCAGTTGCAAGCCTTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCC‑GAAGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTTTT‑CCGGCGTCAGGGTG‑GTG‑TAAATTTTGTTCTTGGTTTCCATTTGCGCCCGCCAGCTAGCttttc                                                                                                                                                                                 >  1:17515/2‑235 (MQ=255)
                                                                  aacccgccgATCCTGTCCACCGCATTACGGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGttctt‑c                                                                                                                                                                      <  1:858070/236‑1 (MQ=255)
                                                                   a‑CCGCCGATCCTGTCCATCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCa                                                                                                                                                                    <  1:794121/239‑1 (MQ=255)
                                                                               tGTCCACCGCATTTCTGCAAGGTTGTGGACAAGGCCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATc                                                                                                                                                         <  1:778538/239‑1 (MQ=255)
                                                                                gTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTTTT‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTTTT‑TCGGAGTCAGGATG‑TTG‑TAAATTTTTTTTTTGGGTTTCATGTTCGCCAGCATGTTAGCTTTTGCCTGTTCTT‑CCCTTTTTTCGa                                                                                                                                                           >  1:128179/1‑236 (MQ=255)
                                                                                     ccGCATTACTGGAAGGTAGGGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCt                                                                                                                                                  <  1:285902/239‑1 (MQ=255)
                                                                                                   ggTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTTAGGAGTTGCAGGCATTTTACCTTTTTCCGCTGGG‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCCTTAAATTTCTTTTTCTGTT‑TCCGGGGCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTTCATGTGCGCCAGCCTGTTAGCTTTTTGCTTTTTTT‑TCATTTTTGCGATC‑TGGGGTTTCCGTTTTACttt                                                                                                                                    >  2:92072/1‑239 (MQ=255)
                                                                                                   ggTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTTCAAGCATTTTTCCTTTTTACGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCCAAATTAGCATTAAATTGCTTTTTTTGTT‑TCGGCGTCGGGATG‑TTG‑TAAATTTTTTTCTGGGGTTCCTTGTGCGCCATCATGTTAGCTTTTCGCTGTTCTTCCCATTTTTG‑GTTC‑TGCCGTTCAGCTTTTTCttt                                                                                                                                    >  1:133897/1‑239 (MQ=255)
                                                                                                     tAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCg                                                                                                                                                            <  1:483229/214‑1 (MQ=255)
                                                                                                      ggttgACAAAACCCGGGGTTTTAAGTGTTTTGGCTGAAAGATTATTCCGCAGTTGCAGGCATTTTTCCTTTTGCCTCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTGCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTGCTTTATc                                                                                                                                 <  2:31759/235‑1 (MQ=255)
                                                                                                       gTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCCAAATTAGCATTTAATTGCTTTTTCTGTT‑CCGGCGTCCGGGTG‑TTT‑GAAAATTTGTTCTGGGGTTTCATTTTGCCCAGCATGTTTACTTTTCGCTGTTTTT‑CCATTTTTTGGGTC‑TTCGCTTTCGCTTTTTCTTTATCg                                                                                                                                >  1:914362/1‑239 (MQ=255)
                                                                                                          gACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTTAGAAGTTGCAGGGATTTTTCCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGTTTTTTTTTTT‑CCGGCGGCCGGGTG‑TTG‑TAAATTTTTTTCTGGGTTTTCATGTGCGCCACCATGTTAGCTTTGCGCTTTTCTT‑CCCTTTTTTCTCTC‑TTCGCTTTCGCTTTTTCTTTTTCGAAg                                                                                                                             >  2:177908/1‑239 (MQ=255)
                                                                                                          gACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTAACTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GAAGCTTCTCAAAATTAGCATTAAATTTCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑GAAATTTTTTTCTGGGTTTCCATTTGCGCCAGCCGTTTTGTTTTTGGCGGTTTTT‑CCATTTTTGCGATC‑TTGGCTTCGGCTTTTACTTTATctgag                                                                                                                             >  1:646786/1‑235 (MQ=255)
                                                                                                          gACAAGACCGGCGGTCTTAAGATTTTTTGCTGAAAAATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCTCTTGA‑CGTTCTG‑TCA‑AACGCTTCTCCAAATTAACATTAAAATGCTTTTTCTTGT‑ACGGCGGCATGTTG‑TTG‑TAAAATTTGATCTTGGTTTCCATGTGGGCCAGCATGTTAACTTTGCGCATTTCTT‑CCATTTTTTCGATCATGC‑CTTCAACTTTTACTTTTTCGaaa                                                                                                                             >  1:647470/1‑238 (MQ=255)
                                                                                                             aaGACCTGCGGTTTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGt                                                                                                                           <  2:637867/238‑1 (MQ=255)
                                                                                                              agacccggtgttttATGTTTTTTGGTGTAAAGATTTTGCAGCCGTTACAGGCATTTTTCCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTTAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTAt                                                                                                                         <  2:133897/227‑1 (MQ=255)
                                                                                                               gACCGGCGGTCTTTAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGGATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGCTG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTTCGCCAGCATGTTAGCTTTGTCCGTTTTTT‑CCATTTTTGGGGTC‑TGCGCTTTAGCGTTTTCTTTTTTGAAGGTAt                                                                                                                         >  1:889933/1‑238 (MQ=255)
                                                                                                               gACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCCAAATTAACATTTAATTGCTTTTTCTGTT‑TCCGCGTCCGGATG‑TTG‑TAAATTTTTTTTTGGGGTTTCCTTTGCGGCCGCCTGTTAGCGTTTCGCTGTTTTT‑TCCTTTTTTTGGTC‑TGCCCTTTACGTTTTACTTTTTCGGAgggaac                                                                                                                        >  2:225099/1‑235 (MQ=255)
                                                                                                               gACCGGCGGTCGTAAGTTTTTTGGCTGAAAGATTATTCAGCAGATTCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTT‑TCA‑GACGGTTCTCAAAATTAACATTAAATTGCTTTTTCTGTT‑CCGCCGTCCGGGTG‑TTG‑TAAATTTTGTTTTGGGTTTTCATTGTCGCCCGCCAGTTGGCTTTTCGCCGTTCTT‑CCCTTTTTTCGCTT‑TGGGCTTCCGCTTTTTCTTTATTGCAGGTAtt                                                                                                                        >  2:836289/1‑238 (MQ=255)
                                                                                                                  cggctgTTTTATTTTTTTTGGGTGAAAGATTATGCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCt                                                                                                                     <  2:552737/239‑1 (MQ=255)
                                                                                                                        tttttaGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCCGGCATTTTTCCTTTTGCCGCTGGA‑CCGTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCaa                                                                                                                <  2:66474/233‑1 (MQ=255)
                                                                                                                        ttttAAGTTTTTTGGTTGAAAGGTTTTTCAGCAGTTGCAGGAATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCaa                                                                                                                <  2:323026/236‑1 (MQ=255)
                                                                                                                        ttctAAGTTTTTTGGGTTAAAGGTTTTTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCaa                                                                                                                <  1:891995/235‑1 (MQ=255)
                                                                                                                        tCTTTAGTTTTTTGGGTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCaa                                                                                                                <  2:586762/238‑1 (MQ=255)
                                                                                                                        tCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCaa                                                                                                                <  1:187490/238‑1 (MQ=255)
                                                                                                                         tttAATTTTTTGGGCTGAAAGTTTTTTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                                                                                              <  2:185017/238‑1 (MQ=255)
                                                                                                                           ttaGTTTTTTGGCTGTAAGTTTTTTCAGCATTTGCAGGCATTTTTCCTTTTGCCGGTTGA‑CGTTCTG‑TCA‑GACGCGTCGTAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatgat                                                                                                            <  2:424309/237‑1 (MQ=255)
                                                                                                                                  tttGGCTGAAAGATTATTCAGCAGTTGCAGGCCTTTTACATTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTCAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTTCGCTGTTCTT‑CCATTTTTGCGATC‑TTCGCTTCAGCTTTTACTTTATCGAAGTGAACGCTAGCAATGATGTCCTGc                                                                                                     >  2:485277/1‑239 (MQ=255)
                                                                                                                                  tttGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCAt                                                                                                       >  1:449612/1‑237 (MQ=255)
                                                                                                                                  tttGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGc                                                                                                     >  2:319014/1‑239 (MQ=255)
                                                                                                                                  tttGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCCCTGTTCTT‑CCATTTTTGCGGTC‑TGCGCTTCAGCTTTTACTTTATGGAAGGTATCGCTGGCAATGATGGCATga                                                                                                     >  2:878432/1‑238 (MQ=255)
                                                                                                                                         taaagtttgtgtcgcttttGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCC‑GAGGCTTCCTAAAATTGGCAGTAAATTGCTTGTTCCGTT‑CCGGCGTCAGGATG‑GTG‑GAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGGTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGTTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTgcgc                                                                                              <  2:73472/222‑1 (MQ=255)
                                                                                                                                         gAAAGATTATTCAGTAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GAAGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATGTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTgcgc                                                                                              <  1:906285/239‑1 (MQ=255)
                                                                                                                                         gAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAAtt                                                                                                                                                                                                                         <  1:124531/118‑1 (MQ=255)
                                                                                                                                         gAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAAtt                                                                                                                                                                                                                         >  2:124531/1‑118 (MQ=255)
                                                                                                                                                    cagcagTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTcc                                                                                   >  2:231589/1‑239 (MQ=255)
                                                                                                                                                    cagcagTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGGCATGCATTGCGCGGCGtttttca                                                                                   >  2:129119/1‑238 (MQ=255)
                                                                                                                                                           tgttggCTTTTTGCCTTTGGCCGTGGGA‑GGTTTTG‑TCA‑GGCGGTGCTCAAAAATAGCATTAAAGTGATTTTTCTTTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTGGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTTCGCTGTTCTT‑CCAGTTTTGCGATC‑TGCGCTTCAGCTTTTTCTTTATCGAAGGTATCGCTGGCAATGATGTCATTCATTGCGCGGCGTTCTCTCAgctgag                                                                            <  2:17515/235‑5 (MQ=255)
                                                                                                                                                           tGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAgcggcg                                                                            <  1:92072/239‑1 (MQ=255)
                                                                                                                                                            gCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGTTTTTTCTGTT‑CCGGCGACAGGATG‑ATG‑TAAATTTTGTTCTGGGGTTCCATGTGCTCCAGCATTTTAGCTTTACGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGTAGGTATCGCTGGCAATGTTATCATCCAtctttctcttatacacctctccgt                                                                            >  1:505563/1‑215 (MQ=255)
                                                                                                                                                            gCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGGGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGGTC‑TGCGCGTCAGCTTTTACTTTATCGAAGGATTCGCTGGCAATGATGGCATGCCTTACGGGGCGTTCTTCCAGcggcgg                                                                           >  2:520840/1‑239 (MQ=255)
                                                                                                                                                                 cATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCACGCGGCGGCCGtt                                                                      >  1:714361/1‑239 (MQ=255)
                                                                                                                                                                     ttACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCAt                                                                  <  1:319014/239‑1 (MQ=255)
                                                                                                                                                                             tGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGTCGGACGGTTCATCTGGTCAc                                                          >  1:134831/1‑239 (MQ=255)
                                                                                                                                                                              gCCGGTGGA‑CCTTCTG‑TCA‑GACGCTTCCCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACg                                                         <  1:225099/239‑1 (MQ=255)
                                                                                                                                                                                ccgcttgcatttttG‑TCA‑GGCGCTTTTTAAAATTTGGATTCAATTGGTTTTTCTGTT‑CCGGCGCTGGGATG‑TTG‑TAATTTTTGTTCTGGTTTTCCATGGGCGGCAGCCTGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGc                                                        <  2:647470/226‑1 (MQ=255)
                                                                                                                                                                                                    gccGCTTTTCAAAATTAGCTTTAAATTGTTTTTTTTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGGTCTGGTTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTcg                                      <  2:909466/237‑1 (MQ=255)
                                                                                                                                                                                                      cGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGTCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTcgcg                                    >  1:554642/1‑239 (MQ=255)
                                                                                                                                                                                                       gCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACCCTGGCCTTTCATGATTTCGCgg                                   >  1:704885/1‑239 (MQ=255)
                                                                                                                                                                                                          tttccaaaTTGCCATTAAATTTTTTTTTTTGTT‑CCGGCCGCTTGATG‑TTT‑TAAATTTTGTTCCTGGTTTCCATTGGCGGCAGCATGTTAGGTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTCCTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATc                                <  2:429897/234‑1 (MQ=255)
                                                                                                                                                                                                           ctcAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCCTGCATTGGGCGGCGTTCTTCCAGTGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCTTGATTTCGGGGATcg                               >  2:320973/1‑238 (MQ=255)
                                                                                                                                                                                                           ctcAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGAttt                                        <  1:607222/230‑1 (MQ=255)
                                                                                                                                                                                                           ctcAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGa                                  >  1:66460/1‑236 (MQ=255)
                                                                                                                                                                                                           ctcAAAATTAGCATTAAATTGCTTTGTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGTTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCACCGGCGGCCTTTTCATCTGGTCACGCTGGCCTTTCTTGATTTCGGGGa                                  >  1:863187/1‑236 (MQ=255)
                                                                                                                                                                                                                     tcttttaatttttttttttttt‑CCGGGTTCAAGTTT‑TTG‑TAAATTTTGTTCTGGGTTTTCTTGTGCGCCAGCATGTTTGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTg                     <  2:134831/227‑1 (MQ=255)
                                                                                                                                                                                                                     gCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatgatg                                                                                                           >  1:733238/1‑153 (MQ=255)
                                                                                                                                                                                                                     gCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatgatg                                                                                                           <  2:733238/153‑1 (MQ=255)
                                                                                                                                                                                                                     gCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGCCGGACGGTTCATCTGGGCACGCTGGCCTTTCATGATTTTGCGGGTctg                              >  2:607222/1‑230 (MQ=255)
                                                                                                                                                                                                                        ttAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTgcg                   <  1:231589/238‑1 (MQ=255)
                                                                                                                                                                                                                          aaaTTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGCCGGACGTTTCATCTGGTCCCGCTGGCCTTTCATGATTTCGCGGGTTTGCTGTTTTTGCGCGt                >  2:248651/1‑239 (MQ=255)
                                                                                                                                                                                                                              ttgtttttCTGTT‑CCGGCGTCAGGTTG‑TTG‑TAAATTTTTTTTTGGGTTTCCATGGGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGt            <  2:128179/236‑1 (MQ=255)
                                                                                                                                                                                                                              ttcTTTTTCTGTT‑GCGGCGTCAGGTTG‑TTG‑TAAATTTTTTTCTGGGTTTCCATGGTCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGt            <  2:714361/237‑1 (MQ=255)
                                                                                                                                                                                                                               ttttttttTGTT‑CCGGCTTGAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTTCTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGtc           <  2:914362/237‑1 (MQ=255)
                                                                                                                                                                                                                               gttttttCTGTT‑CCGGGGTTAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGtc           <  1:889575/237‑1 (MQ=255)
                                                                                                                                                                                                                               gCTTTTTCTGTT‑CCGGCGTTCGGAGG‑TTG‑TAAATTTTGTTGTGGGTTTCCATGTGCGCCAGCATTTTAGCTTTGCTCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGtc           <  1:890255/239‑1 (MQ=255)
                                                                                                                                                                                                                               gCTTTTTCTGTT‑CCGGCGTCAGGATT‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGtc           <  1:433052/239‑1 (MQ=255)
                                                                                                                                                                                                                               gCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGtc           <  1:839606/239‑1 (MQ=255)
                                                                                                                                                                                                                               gCTTTTTCTGTT‑CCGGCGGCAGGATG‑TTG‑TAAATTTTGTTCGGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTG‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGtc           <  1:419575/239‑1 (MQ=255)
                                                                                                                                                                                                                               gCTTTTTCTGGT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGtc           <  1:423688/239‑1 (MQ=255)
                                                                                                                                                                                                                                    ttCTTTT‑CCGGTGTCAGGATG‑TTG‑TAAATTTTTTTCCGGGTTTCCCTTTTCGGCTGCATGTTTGCTTGTCGCTTTTCTT‑CCATTTTTGCTATC‑TTCGCGTCAGCTTTTACTTTATCGAAGGTAGCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTGCATCTGGTCACGCTGGCCTTTCATGATTTCGCTGATCTGCTGTTTCTTCGCGTCGGtcaggtt      <  2:66460/239‑1 (MQ=255)
                                                                                                                                                                                                                                     tCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGCCGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCGGTTTCTGCGCCTCGGtcgggttc     >  1:88145/1‑239 (MQ=255)
                                                                                                                                                                                                                                      ttGTT‑GCGGCGGCTGGTTT‑TTT‑TAAATTTTTTTCTTGTTTTCCCTTTGCGTCCGGTTGGTAACTTTTCGCTGTTTTT‑CCATTTTTGCGATC‑TGCGCTTCGGCTTTTAGTTTATCGAATGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGtcaggttca    <  2:878248/238‑1 (MQ=255)
                                                                                                                                                                                                                                        gTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCAAGCGCCGGACGTTTCATCTGGGTACGCTGGCCTTTCATGATTTTGCGGGTTTGCTGTTTTTGGGGGGCGGggagggtcagg  >  2:419575/1‑239 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   
ACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCA‑GACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTG‑TAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGG  >  NC_000913/1825000‑1825464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑