Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,874,824	C→G	10.5%	T251T (ACG→ACC)	yeaM ←	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,874,824	0	C	G	10.5%	112.4 / 2.5	41	T251T (ACG→ACC)	yeaM	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (11/24);  new base G (3/2);  total (14/27)
Fisher's exact test for biased strand distribution p-value = 3.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTCTGAACTGGAAATCACTGGTGGCGATTGCAGTAGGCGTGATTGTCTCCTGGCTGGGTGGGCGCGGCGTGACGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCC  >  NC_000913/1874589‑1875052                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                     ttttttgattGGAAATCACTTGGGGCGTGTGGGGTAGGCGTGATTGTCCCCGGGCTGGGTGGGCGGGGGGTGACGTTAATGGGCAGCCAGCCGCATCTGGTCGGCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGtt                                                                                                                                                                                                                                   <  2:288516/230‑1 (MQ=255)  ttCTGAACTGGAAATCGCTGGCGGGGATTGCAGTAGGCGTTATTGTCTCCTGGCTGGGTGGGCGCGGCGGGACGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGtt                                                                                                                                                                                                                                   <  1:236865/238‑1 (MQ=255)           ggtaatcgttggtggCGATTGGGGTAGGCGGGATTTTCGCCCGGCTGGGTGGGGGGGGGGGGACGTTAATGGGCAGCCAGCCGGGACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGGTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                                                                                                                                                                                         <  2:379794/230‑1 (MQ=255)           ggAAATCCCTGGTGGCGATTGCAGTAGGCGTGATTGTCTCCTGGCTGGGTGGGCTCGGCGTGACGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                                                                                                                                                                                         <  1:122793/239‑1 (MQ=255)            gAAATCACTGGTGGCGATTGCAGTAGGCGTGATTGTCTCCTGGCTGGGTGGGCGCGGCGTGACGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTGGTTAATCTCGCAATATCTCGGCCTGGGGTTTGGCCCAgg                                                                                                                                                                                                                          >  1:405727/1‑237 (MQ=255)              aacttcTGGTGGGGATTGGGGTGGGCGGGGTTGTCTCCTGGCTGGGGGGGCGGGGCGGGACGGTAATGGGCATCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCCCTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCt                                                                                                                                                                                                                       <  2:346902/233‑1 (MQ=255)                   cTGGTGGCGTTTGCAGTAGGCGTGATTTGCCCCGGGCGGGGGGGGGGCGTCGGGACGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTa                                                                                                                                                                                                                 <  2:818965/239‑1 (MQ=255)                     gggtggggtgggggtggggggtgttGTTTCCTGGGTGGGTGGGCGCGGCGTGACGTTAATGGGCAGCAAGCCGCAACTGGGCGGCGGGCTGCTGGTAGGCTCTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTaaa                                                                                                                                                                                                               <  2:96458/216‑1 (MQ=255)                     gggggCGATTGCAGTAGGCGTGATTGTCTCCTGGCTGGGTGGGCGCGGCGTGACGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTaaa                                                                                                                                                                                                               <  1:250058/236‑1 (MQ=255)                      ggggCGTTTGATGTGGGCGGGTTTGGCTCCTTGCTGGGGGGGCGAGGCGTGACGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGGTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTaaa                                                                                                                                                                                                               <  2:290484/236‑1 (MQ=255)                       tgtgggttGCAGGGGGCGTTGTTGTCCCGTGGCTGGGGGGGGGGGGGGTGGCGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGTTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACa                                                                                                                                                                                                             <  2:780860/233‑1 (MQ=255)                       tgggtgttGCAGTGGGGGTTATTGTCGCCTGGGTGGGGGGGGGCGGGGGGACGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGGGTTTGCCCCAGGCCTTTCTTAAACa                                                                                                                                                                                                             <  2:405727/233‑1 (MQ=255)                          ggttttcgttgggCGTGTTTTTCTCCTGGGTGGGGGGGGGCGGGGTGGCGTTAATGGGAAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATa                                                                                                                                                                                                           <  2:365505/227‑1 (MQ=255)                                     ggCGTGATTGTCTCCTGGCTGGGTGGGCGCGGCGTGACGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGTTTTCTCTGCTTGGGGGGAAACCGTAGTTAATCTCGCCATTAATCGCCCTGGCGTTTGCCCCAGGCCCTTCTTAAAACTAATAAAAAAAGcc                                                                                                                                                                                               >  2:236540/1‑239 (MQ=255)                                           aTTGTCTCCTGGCTGGGTGGGCGCGGCGTGACGTTAATGGGCAGCCAGCCGAAACTGGTCGCCGGGCTGCTGTTAGGCACTGTTTTAGGCGTAGCGCTGTTT‑CGAGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGGCTGGTTTTGCTGATTGCGGGGCATCAGTAGTTAAGCTCGCAATATACCGTGCTGGGGTTTTCCCCCGGCCTTTCTTAAACaaaaaaaaaaagaccagttgg                                                                                                                                                                                         >  2:196283/1‑219 (MQ=255)                                                 tcCTGGCTGGGTGGGCGCGGCGTGACGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCCCTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGGTTGTGGGGAAACAGTCGTTAATCTCGCACTATATCGGCCTGGCGCTTGCCCCAGGCCTTTCTTAAACAAATGAAAAAAGGGCgttgtttacaca                                                                                                                                                                                   >  2:310024/1‑233 (MQ=255)                                                 tcCTGGCTGGGTGGGCGCGGCGTGACGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCa                                                                                                                                                                                   >  2:808719/1‑239 (MQ=255)                                                 tcCTGGCTGGGTGGGCGCGGCGTGACGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGGTT‑CGCCGCGTACCGGTTGGGCCGCTTATTGCCGCGGGGCTGGTTTCGCTGATTGTGGGGAAAACGTGGTTAATCTCGACATATATCCGCCTTGGGTTTGCCCCAGGCCTTCTTTAAACATAGTAATAAAAGCGGTGGTTGAAtta                                                                                                                                                                                   >  2:653474/1‑237 (MQ=255)                                                                   gtcgggccGTTTATGGCCACCCCGCCGCAAGTGGGCGCCGGGCTGGTGGTAGGCATTGTTTTAGGCGTAGCGCTGTTT‑CGCGGTGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCa                                                                                                                                                                 <  2:774522/232‑1 (MQ=255)                                                                   ggcgTGAAGTTAATGGGCAGCCAGCCAGAACTGGTCGCCGTGCTGGTTGTAGGCATTGTTTTAGGCGTAGCTCTTGTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCa                                                                                                                                                                 <  1:418244/239‑1 (MQ=255)                                                                         aCGTTAATGGGCCGGCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACttt                                                                                                                                                            <  1:838059/238‑1 (MQ=255)                                                                                    ccgccagccGCAACTGGTCGCCGGGCTGCTGGTAGGCACTTTTTTAGGCGTAGCGCTGTTT‑CGGGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTAt                                                                                                                                                 <  1:120177/236‑1 (MQ=255)                                                                                        cagccGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGcc                                                                                                                                             <  1:808719/238‑1 (MQ=255)                                                                                               accggggcGCCGGGCTTGTGGTAGGCCCTTTTTTGGGTGTAGCG‑TGTTTGCGGGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACt                                                                                                                                      <  2:519708/231‑1 (MQ=255)                                                                                                  tGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATTGCCTTTCACTAAt                                                                                                                                   >  1:419252/1‑238 (MQ=255)                                                                                                         cGGGCTGCTGGTAGGCCCTGTTTTAGGCGTAGCGTTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAAg                                                                                                                           <  1:310024/239‑1 (MQ=255)                                                                                                            tctgctgGTAGGCACTGTTTTAGGCGTAGCGGTTGTG‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAAgcg                                                                                                                         <  1:286937/237‑1 (MQ=255)                                                                                                                                     gTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGAGTTTTACCAACATTCTTTACAGTATAGCCTTTCCCTAATCCCTGAAGCGCACTAATCAGcgtgaacggcttaca                                                                                                >  1:756107/1‑224 (MQ=255)                                                                                                                                                                  ggcctttttttGCCGGGGGTCTGGTTTCGCTGATTGTGGGGAAACGATGGTTAATCTCGAAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATGCCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCtt                                                                   <  2:794711/230‑1 (MQ=255)                                                                                                                                                                                 cgGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAAGCCCCATAATCAGCTGTAACTGCTGCCGCCACTGGCGAAAACTCAACCCCGTCCCTTTTACTTTCatccgg                                                     >  2:877831/1‑233 (MQ=255)                                                                                                                                                                                  gggTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCACCTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAAGCGCCATAATAAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACCTTCAATCCGGc                                                   >  2:530767/1‑239 (MQ=255)                                                                                                                                                                                    gtttGGTTTCGGTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCGTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGt                                                                        <  1:592668/213‑1 (MQ=255)                                                                                                                                                                                                     gtgGGTAAACAGTTGTTTATCTCGCAATATCTCGGCCTGGGTTTTTCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTTAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCCCTAATCCCTGAAGCGCCATAATCAGCTTTAACTGCTGACGCCCCTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATc                                <  2:539762/239‑1 (MQ=255)                                                                                                                                                                                                                       ccctcgcatttttgccgCCTGGTGTTTTCCCCCGGCCTTTTTTAAACATAGTAATAAAGGCCGTTTTTGAATCATTCCCCAGCGTATGGGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCAt              <  2:419252/223‑1 (MQ=255)                                                                                                                                                                                                                           cGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAAGCGCCATaa                                                                                                                   >  2:614692/1‑134 (MQ=255)                                                                                                                                                                                                                           cGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAAGCGCCATaa                                                                                                                   <  1:614692/134‑1 (MQ=255)                                                                                                                                                                                                                           cGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCCCTGGCGAAAACTCAACCCCGCCTCCTTTACTATCAGTCGGCCAAGATTGCGTTCACTCATCCCCAAAAAGACACCCAAttcc           >  2:144351/1‑236 (MQ=255)                                                                                                                                                                                                                           cGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCTTTCCTTTTACTATCAGTCGGGCACGATTGCGTTCACTCACCCCAAAAACGCCAGCCCATTGc           >  1:403698/1‑238 (MQ=255)                                                                                                                                                                                                                                   atCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGGTGTTGAATCATGCCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGcc  <  1:236540/239‑1 (MQ=255)                                                                                                                                                                                                                                            gCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGc                                           <  1:184693/189‑1 (MQ=255)                                                                                                                                                                                                                                            gCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGc                                           >  2:184693/1‑189 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                     TTTCTGAACTGGAAATCACTGGTGGCGATTGCAGTAGGCGTGATTGTCTCCTGGCTGGGTGGGCGCGGCGTGACGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCC  >  NC_000913/1874589‑1875052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑