Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,260,627 T→C 20.9% E261G (GAG→GGG)  fruA ← fused fructose‑specific PTS enzymes: IIBcomponent/IIC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,260,6270TC20.9% 106.4 / 2.6 54E261G (GAG→GGG) fruAfused fructose‑specific PTS enzymes: IIBcomponent/IIC components
Reads supporting (aligned to +/- strand):  ref base T (18/21);  new base C (10/3);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 6.41e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGT  >  NC_000913/2260392‑2260856
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                      
cAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGACCCCGGCCCtt                                                                                                                                                                                                                                     >  1:403212/1‑239 (MQ=255)
   tttCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTCCCGGCACCATCACCGCCAAGGCTGAACCACCACCAACCCGCCTCAGCGCCCCAACCCACGAACCCGGCACTTaaa                                                                                                                                                                                                                                  >  2:475433/1‑239 (MQ=255)
   tttCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCAACAGCGCCGCAGCCAACGTACCCGGCCCTTTaa                                                                                                                                                                                                                                  >  1:518030/1‑239 (MQ=255)
   tttCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGAACCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAAACTGCATCAGCCCCGCAGCCAACGTAACCGGCCCTTTaa                                                                                                                                                                                                                                  >  1:571848/1‑239 (MQ=255)
     tCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCCGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCCGCCAACGTACCCGGCCCTTTaaa                                                                                                                                                                                                                                 >  1:392746/1‑238 (MQ=255)
     tCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCCCCATCAGAGCAAAGGCTGAACCACCACCAAACCGCACCAGCGCCGCAACCAACGAACCCTGGCCCTTaaaa                                                                                                                                                                                                                                 >  2:378891/1‑239 (MQ=255)
                  gATTAACTTCGAATGTTAACCAGCCAGGAACCCCGAAATAATCCCACCAATGAAGCCAAACCCGGTGCTGCCCGCCAGCATCCCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACcaa                                                                                                                                                                                                                   <  1:254525/239‑1 (MQ=255)
                   aTTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCCGCGCCGCGGCCAACGTACACAGCCCTTTAAACACTTCGATACcaaa                                                                                                                                                                                                                  >  1:645469/1‑239 (MQ=255)
                        cctccacaattgacccagccagAAACCCCCAATATACCCCCCAAAGAAGCCAAAACCCGGTCCTGCCCGCCAGCATGCCGCCAATCAGACCCGGAGTGAGGCCCGGCCGAGCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACcaaaagca                                                                                                                                                                                                              <  2:403212/225‑1 (MQ=255)
                             cAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACAACCACCAATCTGCATCAGCGCCGCAGCCACCGTACCCGGCCCTTTAAACCCTTCGATACCAAAAACAaaagaa                                                                                                                                                                                                        >  1:771460/1‑239 (MQ=255)
                             cAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGAACCGGCACCATCAGCCCACAGACTGAACCACCCCCAACCTGCACCAGCGCCCCACCCACCGAACCCGGCCCTTTAAACGccttcccttatacacatctgacc                                                                                                                                                                                                        >  2:508746/1‑217 (MQ=255)
                                    acccagccagAAACCCCGAAATAATCCACCAAAAGAAGCCGAAACCGGTGCGGACCGCCAGCATGCCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGcgcg                                                                                                                                                                                                  <  1:659487/237‑1 (MQ=255)
                                           cagGAAGCCCGCAATAATACCACCAATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCGGCCAACGTACCCGGCCCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGGGCGATAcaca                                                                                                                                                                                           >  1:112861/1‑239 (MQ=255)
                                             ggAAGCCCGCAATAATACCACCAATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAACCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGCTACCAAAAGCAAAACAAAGCCCGAAAaacaa                                                                                                                                                                                          >  2:263829/1‑237 (MQ=255)
                                                  cccGCAATAATACCACCAATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCCTCAGCGCCGCAGCCACCGAACCCGGCCCTTTAAACACTTCGATACCAAAACCAAAAGAAAACCCGaaaaaaaaacaacc                                                                                                                                                                                    >  2:284756/1‑226 (MQ=255)
                                                    cGCAATAATACCACCAATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGGAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCCATACCAAAAGCAAAGGAAAGCGCGATACACAGACCACCt                                                                                                                                                                                   >  1:489868/1‑238 (MQ=255)
                                                            tccccccaATGAACCCAGTACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGAcc                                                                                                                                                                          <  1:697019/234‑1 (MQ=255)
                                                                 ccaATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAAAAAACGCGAACAACAAACCACCGGCAAAGACCACCgg                                                                                                                                                                     >  2:746006/1‑239 (MQ=255)
                                                                     aggaccccAGAACCGGTGCGCACCCCCAGAATACCGCCAAGCAGCCCCGGAGTGGGGCCCGGACGACCGGAAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCCCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGgcag                                                                                                                                                                  <  2:370129/233‑1 (MQ=255)
                                                                      tGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGgcagc                                                                                                                                                                 >  1:370129/1‑239 (MQ=255)
                                                                             aGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAACAGAAAGCGCGATACCACGCACACCTGCACAGACCATCGGCAGCACGTAAg                                                                                                                                                          >  1:59937/1‑239 (MQ=255)
                                                                                  cggggggaacccGCCAACATGCCACCAACCAGCCCCGGAGGGGGGCCCGGACGATGGGCAAGGGAAAAGGCAATATACCCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGaaa                                                                                                                                                       <  2:42671/228‑1 (MQ=255)
                                                                                     gTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAACGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGTTACCAAAAGCAAAAGAAATCGCGATACCAAAACACCCTGCAACGACCATCGGCAGCATGTAAGAAACGc                                                                                                                                                    >  1:374084/1‑238 (MQ=255)
                                                                                           ccccccaagttactcccataaaGACCCGGGGTGGGGCCCGGACGATCGGCAATGGAAAAGGCAATATACCCTGCCAGTACCGGCCCCAGCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAg                                                                                                                                             <  2:352252/218‑1 (MQ=255)
                                                                                            ccgccgggtacccccaaaacacgcccGGATTGAGGCCCGGACGAGCGGAAATGGAAAAGGCAATAAAACCTGCCAGTACCGGCTCCATCAGCGCAAATGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTGTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAg                                                                                                                                             <  2:489868/215‑1 (MQ=255)
                                                                                                  gattACCGCCATTCAGTCCCGGTGTGAGGCCCGGACGATCGGCAATGGAAAAGACAATTTACCCTGCCAGTACCGGCACCGTCAGCGCAAAGGCTGACCCTCCACCAATCTGCACCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGa                                                                                                                                      <  2:112861/236‑1 (MQ=255)
                                                                                                  cccaccccccAATCAGACCCGGGTTGAGGCCCGGACGATCGGCAAGGGAAAAGGCAAGATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGa                                                                                                                                      <  2:426340/231‑1 (MQ=255)
                                                                                                    aTACCGCCAATCAGACCCGGATTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGAc                                                                                                                                     <  1:402090/238‑1 (MQ=255)
                                                                                                       cccccAACCAGACCCGGGTGGGGCCCCGGACGACTCGGATGGGAAAAGGCAATATAGCCTGCCAGTACCGGCCCCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTa                                                                                                                                 <  2:374084/236‑1 (MQ=255)
                                                                                                       ccGCCAACCAGGCCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTa                                                                                                                                 <  1:387094/239‑1 (MQ=255)
                                                                                                          ccAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATg                                                                                                                               >  1:651044/1‑238 (MQ=255)
                                                                                                            aaacAGGCCCGGAGTTAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATgcgc                                                                                                                            <  1:197266/236‑1 (MQ=255)
                                                                                                                   cccGGTGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATAAAACCTGCCAGTACCGGCACCAGCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCGCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCa                                                                                                                       <  1:710087/237‑1 (MQ=255)
                                                                                                                   cccGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCAct                                                                                                                     <  1:475433/239‑1 (MQ=255)
                                                                                                                   ccaggggTGAGGCCCGGCCGAACGGAAGGAGAAAAGGAAATAAGACCTGCCAGTTCCGGCACCATCAGAGCAAAGGCCAAACCCCCACCAAGCTGCAACAGCGCCTCAGCCAACCTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGc                                                                                                                        <  2:630539/230‑1 (MQ=255)
                                                                                                                     cggggggAGGCCCGGGAGAACGGGAATGGAAAAAGGAAAATAACCCGGCCAGTCCGGTCCCAATAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCAct                                                                                                                     <  1:528602/231‑1 (MQ=255)
                                                                                                                                   ggACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTc                                                                                                      >  1:706492/1‑239 (MQ=255)
                                                                                                                                   ggACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGCAAGCGCCTGCCCTCTCTTTCTTACtttac                                                                                                     >  1:699113/1‑237 (MQ=255)
                                                                                                                                          tCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGCCGGTATGCGCCTGCACTCCCTTTCTTACTTTCAGTgg                                                                                                 >  1:245827/1‑238 (MQ=255)
                                                                                                                                           cGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTc                                                                                               <  1:104809/239‑1 (MQ=255)
                                                                                                                                           cGGCAATGGAAAAGAAAAAAAAACCTGCCAGTACCGGCACCATCACCGAAAAGTCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCTTGCACTCTCTTTCTTACTTTCAGTGGTc                                                                                               <  2:874074/239‑1 (MQ=255)
                                                                                                                                                     aaaGGCAATACAACCTGCCCGTACCGGCCCCACTCGGCCCAAGGCCTAAACCCCCCCAAACTGCATCAGCGCCGCACCCAACGTACCCGGCCCTTTAAACGCTACGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCCCCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAg                                                                                    <  2:245827/239‑1 (MQ=255)
                                                                                                                                                              ataCCCTGCCAGTACCGGCACCATCAGCCAAAAGGCTGACCCACCCCCAATCGGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATCCCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCccg                                                                           <  1:284756/239‑1 (MQ=255)
                                                                                                                                                                      gCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCCCCGGTTc                                                                    >  1:716332/1‑239 (MQ=255)
                                                                                                                                                                      gCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGCTTAAGCTTTGCCCGCCGGTTc                                                                    >  1:604555/1‑239 (MQ=255)
                                                                                                                                                                      gCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGCGCCAGTTTGAGCTTTGCCCGCCGGTTc                                                                    >  1:910352/1‑239 (MQ=255)
                                                                                                                                                                        cAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGGGGTCGCCGTTTGAGATTTGCCCGCCGGTTCAt                                                                  >  1:823791/1‑239 (MQ=255)
                                                                                                                                                                                                aGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCt                                           >  1:660212/1‑239 (MQ=255)
                                                                                                                                                                                                            caccaATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATccctgtct                               >  1:649026/1‑233 (MQ=255)
                                                                                                                                                                                                                cttCTGCATCAGCCCCGCAGCAAACGGCCCCGGCTTTTTAAACGCTTCGAAACCAAAAAAAAAAAAAACCCCGATACACAGACCCCCTGCACGGACCACGGGCAAAATGAAAGAACCGCCCGTCAGCAAGTGCCGGTAGGCGCCTGCCCTCTTTTTCTTCCTTTCAGTGGTCGCCGTTTGATCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTgcg                           <  2:651044/237‑1 (MQ=255)
                                                                                                                                                                                                                    tGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGAATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGcccg                         >  1:346206/1‑237 (MQ=255)
                                                                                                                                                                                                                                 cAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCaa                                                                                                                                                                               <  2:571451/75‑1 (MQ=255)
                                                                                                                                                                                                                                 cAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCaa                                                                                                                                                                               >  1:571451/1‑75 (MQ=255)
                                                                                                                                                                                                                                         gTACCCGGCCCTTTAAACGCTTCGATACCAAAAGAAAAAAAAAGCCGGATACACAGACCACCTGCACCGCCCATCGGCAGCATGTAAGAACCGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGGGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGt  <  1:263829/239‑1 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                      
CAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGT  >  NC_000913/2260392‑2260856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: