Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,975,091	T→G	18.4%	T308P (ACT→CCT)	aas ←	fused 2‑acylglycerophospho‑ethanolamine acyl transferase/acyl‑acyl carrier protein synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,975,091	0	T	G	18.4%	92.7 / 2.4	52	T308P (ACT→CCT)	aas	fused 2‑acylglycerophospho‑ethanolamine acyl transferase/acyl‑acyl carrier protein synthetase
Reads supporting (aligned to +/- strand):  ref base T (24/16);  new base G (6/5);  total (31/21)
Fisher's exact test for biased strand distribution p-value = 7.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAG‑CGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCT‑TCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAAC‑TGGCGGGAAG‑TGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAAACAGCGTTTTCGTAAGCAATTTGCGATAGGAGTCTG  >  NC_000913/2974857‑2975321                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                       tATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAG‑CGAAGATCCATAATTTGTCGGCAGTGGGGACAACTGCTTTTAAATCT‑TCCAGAAAAACCCAAGGCACCTGGGAAAGTTGCTCCGGCAGATGCCAAGGTTTGCCTTTATCCAGAAAA‑TGGGGGGGAG‑GGAAg                                                                                                                                                                                                                                    >  2:718100/1‑239 (MQ=255) tATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGGTTACCCTGTGCCAGACGCGGCATCAGCAAATGAG‑CGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCT‑TCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAAC‑TGGCGGGAAG‑TGAAg                                                                                                                                                                                                                                    <  2:153683/239‑1 (MQ=255)                     tGGCCTTCAGAACCGGAGGTAAAAGGGATCGTCGCCTCTTCTTCCGGCTCCTGTTTACCCTGTCCCAGACGCGGCATCAGCAAATGAG‑CGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCT‑TCCAGATAGACCCAGCGCCCCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAAC‑TGGCGGGAAG‑TGAAGATGGTTTTGATTTCAgccgc                                                                                                                                                                                                                <  1:26672/239‑1 (MQ=255)                      ggCCATCAGACCCGGAGGTAAAAAGGATCAGCCTCTTTTCCTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGAAAATGAG‑CGAAGTCCCATCCTTTGGCGGCAGTGGTGAAATCTGTTTTTAAATCT‑TCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCTTTTATCGAGAAAC‑GGGCGGGAAG‑TGAAGATGGTTTTGATTTCAgccgc                                                                                                                                                                                                                <  1:772584/238‑1 (MQ=255)                          ttCAAAACCGGAGGTAAAAAGGATCAGCGCCCCTTCTCCCGGCTGCTTTTAAACCTGGGCCAGGCGCGGCATCAGCAAATGAG‑CGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCT‑TCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAAC‑TGGCGGGAAG‑TGAAGATGGTTTTGATTTCAGCCGCCGTaa                                                                                                                                                                                                           <  1:779190/239‑1 (MQ=255)                               aaCCGGAGTTAAAAGGATTCACCGCTTCTTCTTCCGGCTGTTTTTTACCCTGGGCCAGCCGCGGCATCAGC‑AATGAGCCAAAGATCCATTCTTTGTCGGCGGTGGTATCATCTGTTTTTAATTCT‑CCCAGATACACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGTTCCCAGAGTTTGCCTTTATCGAAAAAC‑TGGCGGTAAG‑TGAAGATGTTTTTGATTTCAGCCGCCGTAATAGCa                                                                                                                                                                                                      <  2:194546/239‑1 (MQ=255)                                 ccGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAG‑CGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCT‑TCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAAC‑TGGCGGGAAG‑TGAAGATGGGTTTGATTTCAGCCGCCGTAATAGc                                                                                                                                                                                                       >  1:477599/1‑236 (MQ=255)                                    gAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAG‑CGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCT‑TCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAAC‑TGGCGGGAAA‑TGAAGATGGTTTTGGTTTTAACCCCCCTAATTGCACCGGt                                                                                                                                                                                                 >  1:453258/1‑239 (MQ=255)                                      ggTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAG‑CGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCT‑TCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGGTTGCCTTTATCGAGAAAC‑TGGCGGGAAG‑GGAAGATGGTTTTTATTTAAGCCGCCGGAATAGCACTggcgg                                                                                                                                                                                               >  1:712154/1‑236 (MQ=255)                                        tAAAAGGGATCAGCGCCTTCTCTTCCGGGTGCTGTTTAACCTGTCCCAGACGGGGCAACAGCAAATGAG‑CGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTAAAATCT‑TCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAAC‑TGGCGGGAAG‑TGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAg                                                                                                                                                                                              <  1:279045/238‑1 (MQ=255)                                        gaaaaaGGATCAGCGCCTTTTCTTCCGGCTGTTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAG‑CGAAGATCCATACTTTGCCGGCAGTGGTGACATCTGCTTTTAAATCT‑TCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAAC‑TGGCGGGAAG‑TGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAg                                                                                                                                                                                              <  1:501542/237‑1 (MQ=255)                                                  cccccctttttctccCGGCTGTTTTTTAACCGGGCCCAGCGGGGGCATCAGCAAAGGAG‑AAAAGATCCATACTTTGGCGGCAGTGGTGAAATCTGCTTTTAAATCT‑TCCAGATAGACCCGGCGCCCCTGAGAAAGTTGCTCCGGAAGATGCCGGAGTTTGCCTTTATCGAAAAAC‑GGGCGGGAAG‑TGAAGATGTTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTAccc                                                                                                                                                                                   <  2:617049/230‑1 (MQ=255)                                                       ccTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAG‑CGAAGATCCATACTTTGTCGGCAGTGATGACATCTGCTTTTAAATCT‑TCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAAC‑TGGCGGGAAG‑TGAAGATGGTTTTGATTTCAGCCGCCGTAATa                                                                                                                                                                                                         >  1:549841/1‑212 (MQ=255)                                                                  ggcgtgtttttACCCTGTGCCAGACGCGGCAACAACAAATGAG‑CGAGGACCCAATCTTTGTCGGCAGGGGTGACATTTGTTTTCAAATCT‑CCCAGAAAGCCCCAGCGCCCCGGAGTAGGTTGCTCGGGCAGATGCCAGAGTTTGCCTTTAGCGAGAAAC‑GGGCGGGAAG‑TGAAGATGGTTTTGATTTAAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTtcat                                                                                                                                                                   <  2:71431/231‑1 (MQ=255)                                                                              aCCTGTGCCAGACGCGGCATCAGCAAATGAG‑CGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCT‑TCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAAC‑TGGCGGGAAG‑TGAAGCTGGTTTTGGTTTTCACCGCCGTAATAGCACTGGTCCGCCCTTTTACCCCGGCAGTGTAGTTCATCATTTCggg                                                                                                                                                          >  1:840381/1‑236 (MQ=255)                                                                                             ggCATCAGCAAATGAG‑CGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCT‑TCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAAC‑TGGCGGGAAG‑GGAAGATGGGTTTGATTTCAGCCGCCGGAAAAGCACTGGTCAGACCTTTTAACCCGGCACTGGAGTTCATCATTTCGGGCAAGCGGCGACGGGCGag                                                                                                                                        >  2:711071/1‑238 (MQ=255)                                                                                              cttgtgtttataagagaaggtgcccATTCTGTGGCGGGAGTGTGGACATCTGCTTTTAAATCG‑TCCAGATGGCCCCGGCGCACGTGAGTAGTGTGCCGCGGGAGATGCCGGGGTTTGCCTTTATCGAAAATC‑GGGCGGGAAG‑GGAATATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGAt                                                                                                                                        <  2:912940/216‑1 (MQ=255)                                                                                                              cGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCT‑TCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAAC‑TGGCGGGAAG‑TGAAGATGGGTTTTATTTCAGCCCCCGTAAAAGACCCGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCTACATTGCGGGCATGCGGGCACGGGCGATGGCCCCAAAAAACaac                                                                                                                        >  2:152494/1‑237 (MQ=255)                                                                                                              cGAAGATCCATACTTTGTCGGCAGTGCTGACATCTGCTTTTAAATCT‑TCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAAC‑TGGCGGGAAG‑TGAAGATGGTTTTGATTTCAGCCGCCGGAATATCAATGGGCAGCCCTTTTACCCCGGCCGTGTAATTCAGCATTGCGGGCTATCGGCGACCGGCGCTGGCCCCAAAAAacac                                                                                                                         >  1:859366/1‑238 (MQ=255)                                                                                                                aaGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCT‑TCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAAC‑TGGCGGGAAG‑TGAAGATGGTTTTGATTTCAGCCCCCGTAATTGCACTGGGCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCCACGGGCGATGGCCCCAAAAATCAccg                                                                                                                       >  1:908690/1‑236 (MQ=255)                                                                                                                           tttGTCGGCAGTGGTGACATCTGCTTTTAAATCT‑TCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAAC‑TGGCGGGAAG‑TGAAGATGGTTTTGGTTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCGGGGTAGTTCATACTTTCGGGCATGCGGCGACGGGCGATTGCCCCAAAAATCATTGCCCCACTGATg                                                                                                            >  1:149013/1‑238 (MQ=255)                                                                                                                             tGTCGGCAGTGGTGACATCTGCTTTTAAATCT‑TCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAAC‑TGGCGGGAAG‑TGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCCTTGCGGGCATGCGGCGACGGGCGATGGGCCCaaaaaatgtctcttttacacatct                                                                                                          >  1:912940/1‑219 (MQ=255)                                                                                                                             tGTCGGCAGTGGTGACATCTGCTTTTAAATCT‑TCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAAC‑TGGCGGGAAG‑TGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGGCCCAAAAATCACTGCCCCACTGATGccc                                                                                                         >  1:368980/1‑238 (MQ=255)                                                                                                                             tGTCGGCAGTGGTGACATCTGCTTTTAAATCT‑TCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAAC‑TGGCGGGAAG‑TGAAGATGGGTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGGCCCAAAAATCACTGCCGCACTGATg                                                                                                            >  1:748182/1‑236 (MQ=255)                                                                                                                               tCGGCAGTGGTGACATCTGCTTTTAAATCT‑TCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAAC‑TGGCGGGAAG‑TGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCCTGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGc                                                                                                       >  1:800816/1‑239 (MQ=255)                                                                                                                               tCGGCAGTGGTGACATCTGCTTTTAAATCT‑TCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAAC‑TGGCGGGAAG‑GGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTTCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGc                                                                                                       >  1:252640/1‑239 (MQ=255)                                                                                                                                       ggggttacttttttttAAATTTCTCCAGATAGCCCCAGGCCGCTTGAGGAAGTGGCCCGGGAAGTAGCCAGTGTTGTCTTTTAGCGAGAACG‑GGGAGGGAAG‑GGAAGATGGTTGTGATTTAAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTggg                                                                                                 <  2:477599/226‑1 (MQ=255)                                                                                                                                          gACCTCTGATTTTAAATCT‑TCAAGATAGCCCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTACCGAGAACC‑GGGCGGGAAG‑TGAAGTGGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGgcagca                                                                                            <  1:444041/239‑1 (MQ=255)                                                                                                                                                  cTTTTAAATCT‑TCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAAC‑TGGCGGGAAG‑TGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTTCCGCACTGATGCCTGCCTTGGGCAGCATTAAggcc                                                                                    >  1:183860/1‑236 (MQ=255)                                                                                                                                                  cTTTTAAATCT‑TCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAAC‑TGGCGGGAAG‑TGAAGATGGTTTTGATTTCAGCCCCCGTAATAGCACTGGGCAGCCCTTTTACCCCGGCAGTGTTGTTCCTCATTTCGGGCCTGGGGCCACGGGCGATGGCCCCAAAAAACCCTGCCGCCCTGGTGCCTGCCTTGGGGCGCATTAAAccc                                                                                    >  2:161868/1‑238 (MQ=255)                                                                                                                                                          agctccaaGATGGCCCCAGCGCTCCTAAGTAATTTGCCCCGGAAGAGTCCAGGGTTTGCCTTTAGCGAGAACC‑TGGGGGGAAG‑GGAAGTTGTTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGtt                                                                             <  1:189334/232‑1 (MQ=255)                                                                                                                                                            t‑tCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAAC‑TGGCGGTAAG‑CGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGGTCATCATTGCGGGCATGCGGCGACGGGCGATTGCCCCAAAAATCACTGCCCCCCTTAGTCCCGCCTTGGGCAGCATTAAACCGATTCTTTca                                                                           >  1:111688/1‑237 (MQ=255)                                                                                                                                                              tCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAAC‑TGGCGGGAAG‑TGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCCGTGTAGTTCATCATTTCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATACCTGCCTTGGGCAGCCTTAAGCCCATGCGTTca                                                                           >  1:221348/1‑236 (MQ=255)                                                                                                                                                               ccAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAAC‑TGGCGGGAAG‑TGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCCCTGATGCCTGCATTGGGCGGCATTAAACCggacggttggcct                                                                        >  1:112996/1‑227 (MQ=255)                                                                                                                                                                     tgACCCAGCCCCCGTGAGTAAGTTGCCCCGGCAGTTGCCAGAGTTTCCCTTTATCGAAAAAC‑TGGGGGGAAG‑GGAAGGTGGTTTTGATTCCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAaca                                                                  <  1:500702/238‑1 (MQ=255)                                                                                                                                                                     cagcccctcccctcgtggatgtctgctgcGGCAGATTCGTGGTTTTGCCTTTAAGGAAAACG‑TGGGGGGAAT‑GGAAGTGGTTTTTGTATCCAGCCGCCGTAATGGCACTGGTCAGCCCTTTTACACCGGCAGGGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAaca                                                                  <  2:659928/216‑1 (MQ=255)                                                                                                                                                                       aCCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAAC‑TGGCGGGAAG‑TGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCCGCATTAAGCCGATGCGTTCGCCTTCAac                                                                   >  1:113754/1‑236 (MQ=255)                                                                                                                                                                             cgcACCTGCGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAAC‑TGGCGGGAAG‑TGAAGATGGGTTTGATTTCATCCGCCGGAATAGCACTGGGCAGCCCTTTTACCCCGGCCGTGTAGTTCCTCATTGCGGGCATTCAGCGACGGGCGGTGGGCCCCAAAATCTATGCCCCCCTGATCGCTGCATTGGGGGGCACTAAACCAATCCGGTCGCCCTCAAAACCGAAttt                                                          >  2:321771/1‑239 (MQ=255)                                                                                                                                                                             cgcACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAAC‑TGGCGGGAAG‑TGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTTCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTTCCGCACTGATGCCTGCATTGGGCAGCATTAAACCGATGCGTTCGCCTTCAACACTGTAttt                                                          >  1:366576/1‑239 (MQ=255)                                                                                                                                                                                 ccTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAAC‑TGGCGGGGAG‑TGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGTCGACCGGCGATGGCCCCCAAAATCACTGCCGCACTAATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCCCCTTCAacaccggtctctttt                                                      >  1:856904/1‑228 (MQ=255)                                                                                                                                                                                    tggTATGTTGCTCGGGGAGTTGCCAG‑TTTTGTCTTTTACGGAAAACGGGGGGGGAAG‑TAAAGTTGGTTTGGATTTAGACCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAgg                                                   <  2:113754/237‑1 (MQ=255)                                                                                                                                                                                      ttAAGTTGCCCGGGCAGATGCCAGTGTTTGCCTTTACCGAAAAAC‑GGGCGGGAGG‑TGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGa                                                  <  1:161868/237‑1 (MQ=255)                                                                                                                                                                                            tGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAAC‑TGGCGGGAAG‑TGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGGTGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCACCACTGTATTTTTCAAGGATTCGTcc                                           >  1:419835/1‑239 (MQ=255)                                                                                                                                                                                               tCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAAC‑TGGCGGGAAG‑GGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCCATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCACTGGGGAGCATTAAGCCCAGGCGGTCGCCTTCAACACTGGATTTTTCAAGGATGGGGCcaac                                        >  2:86423/1‑239 (MQ=255)                                                                                                                                                                                                         gCCAGTTTTTCCCTTTAGCGAGAAAG‑TGGCGGGAAG‑TGAAGAGTGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAAACAGCGtt                              <  1:718100/239‑1 (MQ=255)                                                                                                                                                                                                                 tttgtttttagcggaAAC‑GGGGGGGATGCTGAATGTGTTTTTGATTTCGGCCGCCGTAAGAGCACTGGTCAGCCCTTTGACCCCGGAAGTGAAGTTCATCATTGCGGGAATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAAACAGCGGTTTCGTaa                       <  2:440560/226‑1 (MQ=255)                                                                                                                                                                                                                  tGCCTTTATCGAGAAAC‑TGGCGGGAAG‑TGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCACTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCCAAGGGCCCAAAAATCATCGCCGCCCACATGCCTGGATCAGGCAGCATTAAGCCAAAGCGTTCGCCCTCAAAACTGTATTTTTCAAGGTAGAGACCaacaaaacacggttaccaaaca                     >  2:254296/1‑223 (MQ=255)                                                                                                                                                                                                                               gaagcgggCGGGAAG‑TGAAGATGTTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAAACAGCGTTTTCGTAAGCAATTTGCGATa          <  1:86423/232‑1 (MQ=255)                                                                                                                                                                                                                                    tGGCGGGAAG‑GGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCCCTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCCCCTTCAAACCTGTATTTTTCAAGGATGGGTCCACCAAAAACCGTTTTCGTAAGAAAttttggtaaggaatt    >  2:899960/1‑227 (MQ=255)                                                                                                                                                                                                                                    gggggggAAG‑TGAAGTTGGTTTTAATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAAACAGCGTTTTCGTAAGCAATTTGCGATAGGAGTc    <  1:711071/235‑1 (MQ=255)                                                                                                                                                                                                                                     ggCGGGAAG‑TGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAACCAGCGTTTTCGTAAGCAATTTGCGATAGGAg      >  1:231637/1‑236 (MQ=255)                                                                                                                                                                                                                                      gCGGGAAG‑TGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACCCTGTATTTTTCAAGGATGCGTCCCACAAACAGCGTTTTCGTAAGCAATTTGCGATAGGGGTCTg  >  1:109149/1‑239 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                       TATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAG‑CGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCT‑TCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAAC‑TGGCGGGAAG‑TGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAAACAGCGTTTTCGTAAGCAATTTGCGATAGGAGTCTG  >  NC_000913/2974857‑2975321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑