Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,206,436 G→A 24.6% intergenic (‑182/‑25) ttdR ← / → ttdA transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4360GA24.6% 55.1 / 3.9 37intergenic (‑182/‑25)ttdR/ttdAtranscriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (2/24);  new base A (2/7);  total (6/31)
Fisher's exact test for biased strand distribution p-value = 2.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGA  >  NC_000913/3206206‑3206650
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                         
tttttaccattcacttgcattttttGGCTAAAGGCCATGTATTCTGCATCGTTAATTAGCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCGTATTCTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAc                                                                                                                                                                                                                   <  2:32104/217‑1 (MQ=255)
   cTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGGGTTAGTTTGTGGCGGGGAAATTTTTCAaaaaaaaaaaaa                                                                                                                                                                                                                >  2:199503/1‑239 (MQ=255)
      ccAGTACCTGCAAGTTTTTTGCTAAAGGCCATCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAAc                                                                                                                                                                                                            <  2:191177/239‑1 (MQ=255)
          tACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTTTGGCGGGGGATTTTTTTTTACAAAAAAAAAAAcaaaa                                                                                                                                                                                                        >  2:895003/1‑235 (MQ=255)
           tccTTCAAGTTTTTGGCTAAAGGCCAGTTTTTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                                                                                                       <  2:258802/238‑1 (MQ=255)
           cccTGAAATTTTTTGGCTAAAGGCCATGTTTTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTTTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                                                                                                       <  1:831677/238‑1 (MQ=255)
            ccttctgttttgtgtcTAAAGGCCAGTTATTCGGAATTGTTTATTATCAGAGTTTGTGATCAGCGCAGTGTATTTCGCAAAACATGGCCAGTCCCTGCTATGGCGCAATATTTATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACAATTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                                                                                                       <  2:312367/223‑1 (MQ=255)
                                   cAGTTATTCAGCATCGGTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                                                                                              <  1:501466/238‑1 (MQ=255)
                                         ttCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                                                                                              <  1:39072/232‑1 (MQ=255)
                                            atcATCGTTAATTTTCCGCATTTGTGATAAGGGCAGTTGATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGTAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                                                                                                    <  2:563084/237‑1 (MQ=255)
                                              cATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                                                                                                     <  1:145456/220‑1 (MQ=255)
                                               aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                                                                                                    <  1:895003/236‑1 (MQ=255)
                                                        tATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATATTTTTCATACAAAGAAAAAAAATGGGTGGGCCTGATGTGGGAAAGAAAAAAACAAAAgaacaagaaaaaaa                                                                                                                                                        >  2:134800/1‑226 (MQ=255)
                                                        tATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGAAGTTTGTGGCGGGGAAGTTTTTTAAACAAAAACAAAAAAAGGAGTTTGCAAGGTGAGGGAAAAtaaaaaaacatgtcctttaaaaaaaa                                                                                                                                                        >  2:145456/1‑219 (MQ=255)
                                                           ccGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCGACAAATAAAAAAACACGGGTTGCCACGATGTGCGGAAGAAAAAATGAACAGACaaggaaaaaaataa                                                                                                                                                     >  1:284148/1‑226 (MQ=255)
                                                                           cgcAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAATCAAATACAAAAAAAAGAGTTTCCATGGTGAGGGCAAGAAATAAACAACAAGAAGTGGATAAAATGAAAGAGAATGTCTCTaa                                                                                                                                     >  1:485897/1‑239 (MQ=255)
                                                                                       cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGa                                                                                                                                                     <  1:297178/211‑1 (MQ=255)
                                                                                                 tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                                                                                                     <  1:112914/169‑1 (MQ=255)
                                                                                                        cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAACAAAAAATGGAGGTGGCCTGATTAGGGAAAGGAATAAACAAAAGGCAGAGGATAAATTGCAAGAGATTTTCGCTAACTTTTCCGCCCTGAtttttcaccgcaag                                                                                                         >  1:200125/1‑227 (MQ=255)
                                                                                                                   cgaataattcattcTTTTTTACGCGTTGATGCACACACTTTTATTTACATAGTAACGGTGGAAAAAATAATAAACCCATATTTTAAGTGATATTTTGGGGCGGTAATTTTTTCTAGACAAATAAAAAAACGGAAGTTGCCATGATGGTCAAAATTAATAAGCAACAGGAAGTAAATAGTTTGACAGATATTGTCGCTACTTTAACCGCCATGATTTCTACCCGAATGCCTGATGACgt                                                                                              <  1:773826/232‑1 (MQ=255)
                                                                                                                        ttttttttttttttttgggttaaaTTCACAAACTTTAAATTAAATTTTCAGGGTGAAAAAAAAAAAAAACCCACATTTTAAGTGGTAGTTGGGGGGGGTGAATTTTCCAAAAAAAAAAAAAAAACTGGAGTTCCCAGGATGAGAAAAATAAATAAGCAACAGGAAGTAAATAAGTTAACAGAGATTGCCGCAAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                        <  2:200125/216‑1 (MQ=255)
                                                                                                                        ttttttttttttttttCGTTTTTATTCACAATTTTTTAATTCAATATTGGCGGTGGAAAAAAATAAAAACCAACATTTTTAGTGGTAGTTTGGGGGGGTGAATTTTTCAAGAAAAATACAAAAAGTGGTGTTGCAATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGATATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                        <  2:257161/227‑1 (MQ=255)
                                                                                                                        tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                                                                                              <  1:87853/153‑1 (MQ=255)
                                                                                                                                  tATTTTGGTGTTGATTCACGAACTGTTAATTACATTTTCACGGGGGAAAAAAATATAAACCCAAATTTTTGGGGGTAGTTGGGGGCGGTGAATTTTCCCAGAAAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                              <  1:729240/239‑1 (MQ=255)
                                                                                                                                                                  aagtatccgggtgtaaaaaaaTAAAAACCCCCATTTTTTGTGTTAGTTTGTGGCGGTTAATTTGTCCAAACAAATACAAAAACTGGGTTTGCTATGATGAGCGAAATTAATAAGCAACAGGCATTGAATAAGTTGCCAGAGATTGTCGCAAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATggg                                              <  1:648869/224‑1 (MQ=255)
                                                                                                                                                                    ttttgagggtgaaaaaaaaTAAAAAACCACATTTTTAGTGTTTTTTTGGGGGGGTAAATTTTACCAGACAAATAAAAAAACTGGAGTTGCCATGATGAGGAAAAGAAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                            <  1:689672/226‑1 (MQ=255)
                                                                                                                                                                    tatTCGCGGGTGAAAAAAACAAAAACCATATTTTTGATGGTTAGTTGTGGGCGGGGAATTTCTCAAAACAAAAACAAAACGTGGATTGCCAAGGATGAGCAAAATTAAAAAGCAACGGGAAGTAAATAAGTGGACAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                            <  1:646094/239‑1 (MQ=255)
                                                                                                                                                                           gggtaaaaaaaaaaaaaaaaCAAATTTTGGGTGGTTTGTTGGGGGGTAGAATTTTTCAAGAAAAATCAAAAAAGTGAGGTGGCCATGTGGAGGAAAATAAATAACAAACGGGTAGTGAATAGGTGAAAGGAGATTGTCGTTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                     <  2:752705/233‑1 (MQ=255)
                                                                                                                                                                           ggggaaaaaaaaTAAAAACCCCAGTTTTGAGTGGGTGTTTGGGGCGGGGAATTTTTCCAGCAAAATAAAAAACCTGGAGTTGCCAGGATGATCGAAATAAACAACCAGCAGTCTGTAAATAATTTGACAGAGTTTGTCGCTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                     <  1:134800/233‑1 (MQ=255)
                                                                                                                                                                            ggtggaaaaaaTTAAAAACCAAATTTTTATTGTGTAGGTTGTGGGGTTAATTTTTTCGAAGAAAAGAAAAAAACTGAAATTTCCATGAAGTGCGAAATTAAAAGGAAATATGGAGTGAAAAAGTTGAAAGAGTTTGTCCTCACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                     <  1:363412/234‑1 (MQ=255)
                                                                                                                                                                            gTGGAAAAAAATAAAAAACCAATTTTTTAGTGGTAGTTTGGGGCGGTAAATTTTACCAGAAAAATAAAAAAACTGGGTTTGCCATGATGAGAGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGAAAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                     <  1:577720/238‑1 (MQ=255)
                                                                                                                                                                                     ataataAAACCAATTTTTTAGTGGGATTTGGGGGGGGGAAATTTTTCAAAAAAAGAAAAAAAACTGGAGTGGCAATGAGGAGAAAAAGTAATAAGCAACTGGAAGTGAATAAGTTGAAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACg                           <  2:284148/236‑1 (MQ=255)
                                                                                                                                                                                                  aTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGATCGAAAGTAATAAGCAACAGGCAGTGaa                                                                                                                                                             <  1:396355/96‑1 (MQ=255)
                                                                                                                                                                                                   ttttttAGTGGTAGTTGGGGGGGGTAAATTTTCCCAGACAAAAACAAAAACTGGATTTGCCATGAGGAGCGAAAGTAATAACCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGacaaca              <  1:919112/238‑1 (MQ=255)
                                                                                                                                                                                                    attttttttggggttgttGTGGGGGGTAAATTTTCAAAAAAAAAAAAAAAAGCTGGATTTGCATGGATGAGGAAAAGAAATAAGCAACGGGAAGTAAATAAGTTAAAAGGGTTTGGCGAAAATTTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGacaa                <  2:823911/223‑1 (MQ=255)
                                                                                                                                                                                                            ggTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAAAAGGCAGTGAATAAGTTAGAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCaaaa       <  1:605525/237‑1 (MQ=255)
                                                                                                                                                                                                                 aGTTGGGGGGGGTAAATTTTTCCAGACAAATAAAAAAAGTGGAGGTGCCGTTATGGGCAAACGTAACAAGCAACAGGCAGTGAATAAGTTGACAGAGTTTGCCGCTATCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa  <  1:164983/239‑1 (MQ=255)
                                                                                                                                                                                                                  gTTGGTGGGCGTGAATTTTACAAAAAAAATACAAAAACGGGAGGTGCAAGAGGAGCGGAAATTAAAAAGCAACGGGCACGAAATAGTTGGACGGAGATTGGCGCAACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa  <  1:733821/238‑1 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                         
TTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGA  >  NC_000913/3206206‑3206650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: