Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,206,475 T→A 27.4% S5R (AGT→AGA ttdA → L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4750TA27.4% 48.9 / 3.3 32S5R (AGT→AGAttdAL‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (5/17);  new base A (3/6);  total (9/23)
Fisher's exact test for biased strand distribution p-value = 6.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGT  >  NC_000913/3206241‑3206708
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                         
cAGTTATTCAGCATCGGTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                                                                                                                                                        <  1:501466/238‑1 (MQ=255)
      ttCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                                                                                                                                                        <  1:39072/232‑1 (MQ=255)
         atcATCGTTAATTTTCCGCATTTGTGATAAGGGCAGTTGATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGTAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                                                                                                                                                              <  2:563084/237‑1 (MQ=255)
            aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                                                                                                                                                              <  1:895003/236‑1 (MQ=255)
                     tATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATATTTTTCATACAAAGAAAAAAAATGGGTGGGCCTGATGTGGGAAAGAAAAAAACAAAAgaacaagaaaaaaa                                                                                                                                                                                                                  >  2:134800/1‑226 (MQ=255)
                     tATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGAAGTTTGTGGCGGGGAAGTTTTTTAAACAAAAACAAAAAAAGGAGTTTGCAAGGTGAGGGAAAAtaaaaaaacatgtcctttaaaaaaaa                                                                                                                                                                                                                  >  2:145456/1‑219 (MQ=255)
                        ccGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCGACAAATAAAAAAACACGGGTTGCCACGATGTGCGGAAGAAAAAATGAACAGACaaggaaaaaaataa                                                                                                                                                                                                               >  1:284148/1‑226 (MQ=255)
                                        cgcAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAATCAAATACAAAAAAAAGAGTTTCCATGGTGAGGGCAAGAAATAAACAACAAGAAGTGGATAAAATGAAAGAGAATGTCTCTaa                                                                                                                                                                                               >  1:485897/1‑239 (MQ=255)
                                                    cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGa                                                                                                                                                                                                               <  1:297178/211‑1 (MQ=255)
                                                                     cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAACAAAAAATGGAGGTGGCCTGATTAGGGAAAGGAATAAACAAAAGGCAGAGGATAAATTGCAAGAGATTTTCGCTAACTTTTCCGCCCTGAtttttcaccgcaag                                                                                                                                                                   >  1:200125/1‑227 (MQ=255)
                                                                                cgaataattcattcTTTTTTACGCGTTGATGCACACACTTTTATTTACATAGTAACGGTGGAAAAAATAATAAACCCATATTTTAAGTGATATTTTGGGGCGGTAATTTTTTCTAGACAAATAAAAAAACGGAAGTTGCCATGATGGTCAAAATTAATAAGCAACAGGAAGTAAATAGTTTGACAGATATTGTCGCTACTTTAACCGCCATGATTTCTACCCGAATGCCTGATGACgt                                                                                                                                                        <  1:773826/232‑1 (MQ=255)
                                                                                     ttttttttttttttttgggttaaaTTCACAAACTTTAAATTAAATTTTCAGGGTGAAAAAAAAAAAAAACCCACATTTTAAGTGGTAGTTGGGGGGGGTGAATTTTCCAAAAAAAAAAAAAAAACTGGAGTTCCCAGGATGAGAAAAATAAATAAGCAACAGGAAGTAAATAAGTTAACAGAGATTGCCGCAAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                  <  2:200125/216‑1 (MQ=255)
                                                                                     ttttttttttttttttCGTTTTTATTCACAATTTTTTAATTCAATATTGGCGGTGGAAAAAAATAAAAACCAACATTTTTAGTGGTAGTTTGGGGGGGTGAATTTTTCAAGAAAAATACAAAAAGTGGTGTTGCAATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGATATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                  <  2:257161/227‑1 (MQ=255)
                                                                                     tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                                                                                                                                                        <  1:87853/153‑1 (MQ=255)
                                                                                               tATTTTGGTGTTGATTCACGAACTGTTAATTACATTTTCACGGGGGAAAAAAATATAAACCCAAATTTTTGGGGGTAGTTGGGGGCGGTGAATTTTCCCAGAAAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                        <  1:729240/239‑1 (MQ=255)
                                                                                                                               aagtatccgggtgtaaaaaaaTAAAAACCCCCATTTTTTGTGTTAGTTTGTGGCGGTTAATTTGTCCAAACAAATACAAAAACTGGGTTTGCTATGATGAGCGAAATTAATAAGCAACAGGCATTGAATAAGTTGCCAGAGATTGTCGCAAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATggg                                                                                                        <  1:648869/224‑1 (MQ=255)
                                                                                                                                 ttttgagggtgaaaaaaaaTAAAAAACCACATTTTTAGTGTTTTTTTGGGGGGGTAAATTTTACCAGACAAATAAAAAAACTGGAGTTGCCATGATGAGGAAAAGAAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                      <  1:689672/226‑1 (MQ=255)
                                                                                                                                 tatTCGCGGGTGAAAAAAACAAAAACCATATTTTTGATGGTTAGTTGTGGGCGGGGAATTTCTCAAAACAAAAACAAAACGTGGATTGCCAAGGATGAGCAAAATTAAAAAGCAACGGGAAGTAAATAAGTGGACAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                      <  1:646094/239‑1 (MQ=255)
                                                                                                                                        gggtaaaaaaaaaaaaaaaaCAAATTTTGGGTGGTTTGTTGGGGGGTAGAATTTTTCAAGAAAAATCAAAAAAGTGAGGTGGCCATGTGGAGGAAAATAAATAACAAACGGGTAGTGAATAGGTGAAAGGAGATTGTCGTTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                               <  2:752705/233‑1 (MQ=255)
                                                                                                                                        ggggaaaaaaaaTAAAAACCCCAGTTTTGAGTGGGTGTTTGGGGCGGGGAATTTTTCCAGCAAAATAAAAAACCTGGAGTTGCCAGGATGATCGAAATAAACAACCAGCAGTCTGTAAATAATTTGACAGAGTTTGTCGCTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                               <  1:134800/233‑1 (MQ=255)
                                                                                                                                         ggtggaaaaaaTTAAAAACCAAATTTTTATTGTGTAGGTTGTGGGGTTAATTTTTTCGAAGAAAAGAAAAAAACTGAAATTTCCATGAAGTGCGAAATTAAAAGGAAATATGGAGTGAAAAAGTTGAAAGAGTTTGTCCTCACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                               <  1:363412/234‑1 (MQ=255)
                                                                                                                                         gTGGAAAAAAATAAAAAACCAATTTTTTAGTGGTAGTTTGGGGCGGTAAATTTTACCAGAAAAATAAAAAAACTGGGTTTGCCATGATGAGAGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGAAAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                               <  1:577720/238‑1 (MQ=255)
                                                                                                                                                  ataataAAACCAATTTTTTAGTGGGATTTGGGGGGGGGAAATTTTTCAAAAAAAGAAAAAAAACTGGAGTGGCAATGAGGAGAAAAAGTAATAAGCAACTGGAAGTGAATAAGTTGAAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACg                                                                                     <  2:284148/236‑1 (MQ=255)
                                                                                                                                                               aTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGATCGAAAGTAATAAGCAACAGGCAGTGaa                                                                                                                                                                                                                       <  1:396355/96‑1 (MQ=255)
                                                                                                                                                                ttttttAGTGGTAGTTGGGGGGGGTAAATTTTCCCAGACAAAAACAAAAACTGGATTTGCCATGAGGAGCGAAAGTAATAACCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGacaaca                                                                        <  1:919112/238‑1 (MQ=255)
                                                                                                                                                                 attttttttggggttgttGTGGGGGGTAAATTTTCAAAAAAAAAAAAAAAAGCTGGATTTGCATGGATGAGGAAAAGAAATAAGCAACGGGAAGTAAATAAGTTAAAAGGGTTTGGCGAAAATTTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGacaa                                                                          <  2:823911/223‑1 (MQ=255)
                                                                                                                                                                         ggTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAAAAGGCAGTGAATAAGTTAGAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCaaaa                                                                 <  1:605525/237‑1 (MQ=255)
                                                                                                                                                                              aGTTGGGGGGGGTAAATTTTTCCAGACAAATAAAAAAAGTGGAGGTGCCGTTATGGGCAAACGTAACAAGCAACAGGCAGTGAATAAGTTGACAGAGTTTGCCGCTATCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                            <  1:164983/239‑1 (MQ=255)
                                                                                                                                                                               gTTGGTGGGCGTGAATTTTACAAAAAAAATACAAAAACGGGAGGTGCAAGAGGAGCGGAAATTAAAAAGCAACGGGCACGAAATAGTTGGACGGAGATTGGCGCAACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                            <  1:733821/238‑1 (MQ=255)
                                                                                                                                                                                                            aTACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGCGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGTTGGGGAAAATTATCTTCCATACGATTTTCGACAACATCCAAAAAGCGATTAGCCTGAATCGTCCTGCCTTACAGGa                                >  1:862013/1‑238 (MQ=255)
                                                                                                                                                                                                            aTACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGACTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACGACATGCAAAAACCGATTGACCTGAATCGCCCTGCCTGCCAgaa                                >  1:879339/1‑236 (MQ=255)
                                                                                                                                                                                                                   aaCGGGAGTTGCCATGAGGAGCAAAATTAAAAAGAAAAGGGCATTGAATAGGTGGAAAGGGTTTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                        <  1:840587/239‑1 (MQ=255)
                                                                                                                                                                                                                      aacgggagttcccaggtgtgacaaaagaaaaaagaaaCAGTCAATGAATAGGTATCCAGATATGGCCCTAAATTTTACCGACATGATGTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                        <  2:727043/216‑1 (MQ=255)
                                                                                                                                                                                                                         gAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGAtt                   >  1:10664/1‑239 (MQ=255)
                                                                                                                                                                                                                                          cGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACg                                                                                     >  1:551746/1‑157 (MQ=255)
                                                                                                                                                                                                                                          cGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAAAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTCCGACAACATGCAAAAACCGATTGACCTGAATCGACCTGCCTGTCGGAAAAACGGGGAGATTAAGTTTTTCGTaaaa  >  2:460051/1‑238 (MQ=255)
                                                                                                                                                                                                                                             aaaGAAATCAGAAACGGGCAGGAAAAAGGTTGCCAGATGTTTTCGTAATATTTACCGCCATGATTTATACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGt  <  1:671095/238‑1 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                         
CAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGT  >  NC_000913/3206241‑3206708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: