Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,239,394	C→T	13.8%	L273L (CTG→TTG)	alx →	putative membrane‑bound redox modulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,239,394	0	C	T	13.8%	188.3 / 2.8	72	L273L (CTG→TTG)	alx	putative membrane‑bound redox modulator
Reads supporting (aligned to +/- strand):  ref base C (10/51);  new base T (6/5);  total (16/56)
Fisher's exact test for biased strand distribution p-value = 1.16e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATTCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTG  >  NC_000913/3239156‑3239627                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                               tttgCGTAAGAATGGATTGCTGGACGCCACACCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATTCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTc                                                                                                                                                                                                                                                <  1:554172/237‑1 (MQ=255)           agtgggtttggcttcccacaacCGCTGTTGGTGGTACGGTGTCTGGGTGAATTGGGCGGCGTGTATTTCGCCGTGGATCGCATTCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTAt                                                                                                                                                                                                                                      <  2:908121/219‑1 (MQ=255)           aaTGGATTGCTGTACGCCACTCCGCGGATGCTGGTAGTGATGCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATTCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTAt                                                                                                                                                                                                                                      <  1:893462/239‑1 (MQ=255)            aTGGTTTGCTTTCCCCCACCCCGGTGTTGGTGGTTTGGATTCTGGGGGAATTGAGCGACGTGTTTTTCGCCGGGGATAGCATTCCGGCTACCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTAt                                                                                                                                                                                                                                      <  2:61897/238‑1 (MQ=255)                  tGCTGGACGCCACCCCGCTGATGCTGGTACTGATTCTGGTGGAATTGGGCGACGTGATTTTCGCCGTGGATAGCATTCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATc                                                                                                                                                                                                                               <  1:761741/239‑1 (MQ=255)                         cGCCACACCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATTCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCAGGGAGGTTTTTGTGGCCCAAATTTGGCCCGGC‑GGTGGTTTTgggggttttatggttacaaaagc                                                                                                                                                                                                                        >  2:316241/1‑218 (MQ=255)                              cacCGCTGGTGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATTCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGCAGCCGAGCGCTTCTCGATGGCCAAAACTTGCCTGGC‑GGGGGTTTTCGGGTTTTTCgggtgcaaaatccctgtt                                                                                                                                                                                                                   >  1:400097/1‑223 (MQ=255)                               cccGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATTCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTg                                                                                                                                                                                                                  <  1:439721/238‑1 (MQ=255)                                 tgttgtgggtGGTCCTGGTTCGGGTGGAATTGGGCGGGGTGGTTTTCGCCGTGGATAGCATTCCGGCTAGCTTCGGCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAGCCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTc                                                                                                                                                                                                                <  2:498494/230‑1 (MQ=255)                                 cccttgtggtgggcttGAGTCTGGTGGAATTGAGCGACTTGATTTTCGCCGTGGATAGCAGTCCGTCTATCTTCGCCGTGCCCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTc                                                                                                                                                                                                                <  2:176497/224‑1 (MQ=255)                                 cGCTGATGCTGTTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATTCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTc                                                                                                                                                                                                                <  1:31041/239‑1 (MQ=255)                                      gttgtgtttcttgttttggtggAATTGGGCGACGTTATTTTCGCTGTGGATAGCATTCCGGCTCTCTTGGCCGTGACCACTGACCCGTTCATGGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACtt                                                                                                                                                                                                           <  2:124996/223‑1 (MQ=255)                                      aTGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATTCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGGACCAAGGCGGTTCTTGGTGCTCCAATATGGCCTTGC‑GGGGGGTTTGGGGTTTTTCGGTATCTAAATGCTCGTTTTCGACtt                                                                                                                                                                                                           >  1:594722/1‑239 (MQ=255)                                           tgttcTGTTTGTGGTTGAATTGGGGGACGTGGTTTTCGCCGTGGATAGCATTCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTAcc                                                                                                                                                                                                      <  2:400097/235‑1 (MQ=255)                                           ggTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATTCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCAGGGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGGGAGTCTGGGGGTTTTTGGGGTCAAAGTTGTCATTGGTGACTTCTAcc                                                                                                                                                                                                      >  1:357520/1‑239 (MQ=255)                                                          ggtattggggggcGTGTTTTTCGCCGTGGATTGCTTTCCGGCTATCTTCGCCGTGACCCCTGACCCGTTCATTGGTCTGACCACAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGcc                                                                                                                                                                                        <  2:291644/226‑1 (MQ=255)                                                                      tgtttttaggggcggggATTGCTTTCCGGCTTTCTTCCGCGGTACCCTCGACCCGTTCATTGTGCTGACCCCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCg                                                                                                                                                                           <  2:78268/224‑1 (MQ=255)                                                                       gtgtTTTCCGCCGTGGTTTGCATTCCGGCTATCTTGGCCGTGCCCCCTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCg                                                                                                                                                                           <  2:411822/238‑1 (MQ=255)                                                                        tttttttCGCCGTGGTTTGCATTCCGGCTATCTTCGCCGTGACCACTTACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCTTGCGGGCGATGTATTTCCTGCTGGCGGGCGCAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCgtg                                                                                                                                                                         <  2:149724/236‑1 (MQ=255)                                                                         gATTTTCGCCGTGGATAGCATTCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCgtg                                                                                                                                                                         <  1:125612/238‑1 (MQ=255)                                                                             ttgccggTGGTTTGCCTTCCGGCTTCTTTCGCCGTGACCACTGACCCGTTTATTGTGCTGACCCCAAACCTGGTTGCGATCCGCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGtt                                                                                                                                                                    <  2:8054/233‑1 (MQ=255)                                                                                            ttttccgtttttttCGCCGTGCCCCCTGACCCGTTTATTGGCCGGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGTGCGTAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGtgat                                                                                                                                                     <  2:317777/227‑1 (MQ=255)                                                                                            aTTCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGtgat                                                                                                                                                     <  1:871189/239‑1 (MQ=255)                                                                                             tttcgttttttttCGCCGTGACCCCTTGCCCGTTTATTGTGCGGACCTCAAACCTGTGTGCGATCCTCGGGCTGGGTGCGATGTATTTCCTGGTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGtgatg                                                                                                                                                    <  2:18402/228‑1 (MQ=255)                                                                                                cGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGGTTCTCGATGCTCAAATATGGCCTGGC‑GGGGATTCTGGGGGTTTTCGGGATCAAGATGCTGTTTGTCGACTTCTACCCTATTTCAATCGCCGTCTCGCTGGGCGGGGGGTTTGGcctgttccttatacac                                                                                                                                                 >  1:78268/1‑224 (MQ=255)                                                                                                                gACCCCTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGcc                                                                                                                                <  1:860098/238‑1 (MQ=255)                                                                                                                gACCACTTACCCGTTCATTGTGCTGACCGCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGcc                                                                                                                                <  1:741430/238‑1 (MQ=255)                                                                                                                          ccGTTCATTGTGCTGCCCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTtta                                                                                                                                            <  1:170415/217‑1 (MQ=255)                                                                                                                           cgttttttGTGCTGGCCTTAAACCTGTTTGCGATCCTGGGTCTGCGGGCGATTTATTTCCGTCGGGCGGGCGTAGCAGGGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCGGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTAt                                                                                                                    <  2:663807/233‑1 (MQ=255)                                                                                                                             ttttttttgttgcccTCAAGCCTGTTTGGGGTCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCg                                                                                                                  <  2:63773/226‑1 (MQ=255)                                                                                                                               tcttGTGCTGACCTTAAACCTTTTTGCGTTTCTGGGCTTGCGTTCGATGGATTTCCTGCTTGCGGGCGTAGCAGGGCTTTTCTCGATGCTCAAATTTGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGc                                                                                                                <  2:383549/237‑1 (MQ=255)                                                                                                                                aTTGTGCTGACCTCAACCCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCa                                                                                                               <  1:668047/239‑1 (MQ=255)                                                                                                                                                     tttGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCTATGCTCAAATATGGCCTGGC‑GGTGATTCTGGGGGTTATCGGGATCAAGATGCTGATTGTCGACTTCTACCCTATTCCAATCGCCGTCTCGCTGGGCGTGGGGTTTTGCATTCTGGTGATG‑ACGTTTATTT‑TCAAAGCCTGGGTGGATTATCGGCATTGT‑AA‑G‑GAGAGGGGGGGGTa                                                                                       >  1:619296/1‑239 (MQ=255)                                                                                                                                                     tttGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAATATTGCCTGGCGGGGGGTTTGGGGGTTTTTCGG‑ATCCAGATCGTGATTTTCGACTTTTTACCTATTCCAAACGCCCTCTCCCTGGGGGGGGGGTTTTGCATTTTGGTGATGTACGTTT‑TTT‑TCCACCCCTGGGGGGAATTTCGGCCTGATGAA‑G‑AGGCGggggggga                                                                                        >  2:605745/1‑237 (MQ=255)                                                                                                                                                             cctgcgcttgggttgtgtGTTTTTCCTGCTGGCGGGCGTAGCAGGGCGTTTCCGGATGCTCAAATATGGCCTGGC‑GGTGATTTTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTaa                                                                               <  2:882342/222‑1 (MQ=255)                                                                                                                                                             cccctgCCTGCGTGGGATTTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCGCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATAGTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑GCGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTaa                                                                               <  1:373610/234‑1 (MQ=255)                                                                                                                                                             ccTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTaa                                                                               <  1:541691/239‑1 (MQ=255)                                                                                                                                                             ccTCGGCCTGCGTGCGATGTATTTCCGGGTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTaa                                                                               <  1:5734/239‑1 (MQ=255)                                                                                                                                                             ccTCGGCCTGCGTGCGATGTAATTCCTGGTGGCGGGCGTAGCAGAGCGTTTCGCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCCGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTaa                                                                               <  1:156424/239‑1 (MQ=255)                                                                                                                                                             ccTAGGCCTGCGTGGGATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTAAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTaa                                                                               <  1:360719/239‑1 (MQ=255)                                                                                                                                                              cctggCCTGGTTGCGGTGTTTTTCCGGCTGGCGGGCGTAGACGTGTGTTTCTCGATGCTCAAAGATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTAAt                                                                              <  2:35131/236‑1 (MQ=255)                                                                                                                                                                    cttcgtgcgGGGTTTTTCCTGGTGGCGGGCTTTGCAGAGGGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTAATCTGCCt                                                                        <  2:594722/236‑1 (MQ=255)                                                                                                                                                                    cTGCGTGCGATGTTGTTCCGGCTGGCGGGCGTAGCAGAGTGTATCTCGAAGCCCAAATATGGTCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTAATCTGCCt                                                                        <  1:390582/239‑1 (MQ=255)                                                                                                                                                                     tgggtgggATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTAATCTGCCt                                                                        <  1:605745/235‑1 (MQ=255)                                                                                                                                                                     tgcgtgcgATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTAATCTGCCt                                                                        <  1:110598/238‑1 (MQ=255)                                                                                                                                                                      gcgtgcgATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTAATCTGCCTaa                                                                      <  1:248321/239‑1 (MQ=255)                                                                                                                                                                       cgtgcgATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTTTCGATGGTCAAATATGGCCTGGC‑GGGGATTTTGGGGTTTATTGGTATCAAGATGTTGGTTGTCGGCTTCTTACAAATTTCCAACGCCGGCTCGCTGGGGGGGGGGGTTGGGCTTCGGGGGATG‑ACGTTTATTT‑TTAACCCCGGGGGGGATTATCGGCGTGGT‑AA‑GACCGCGGGGGGGAAAATTTTAATTTTGCCaaa                                                                      >  2:818289/1‑239 (MQ=255)                                                                                                                                                                       cgtgcgATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGGTGCTCAAATTTGGCCTGGC‑GGTGGTTTTGGGGTTTATTGGGATCAAGATCGTGAATTTCGGCTTTCTCCCTATTTCCATTGCCCTCTTGCTGGGGGTGGGGGTTGGCACTCTTGTGGTG‑ACGTTTTTTATTCAAC‑CCTGGGGTGATTATTGGGCTGGT‑AACG‑CACGGGGGGGGTAAATTTTTatttcgctaag                                                                      >  2:667135/1‑229 (MQ=255)                                                                                                                                                                        ctgctattgaTTTCCTGCTGCCGGGCGCAGCAGGGCCTTTCTCGATGCTGAAATATGGCCTGGC‑GGTAATTCTGGTGTTTATCGGTAACAAGATTCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTaa                                                                               <  1:625169/219‑1 (MQ=255)                                                                                                                                                                               gTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTa                                                              <  1:316241/238‑1 (MQ=255)                                                                                                                                                                                 aTTTCCTGCTGGGGGGCGTAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTAGCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTAccc                                                           <  1:520154/239‑1 (MQ=255)                                                                                                                                                                                 aTTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTAccc                                                           <  1:818289/239‑1 (MQ=255)                                                                                                                                                                                                 ccgaGCAGGGCGGTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTTCGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAg                                            <  1:706507/235‑1 (MQ=255)                                                                                                                                                                                                    aGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCAc                                         <  1:252731/238‑1 (MQ=255)                                                                                                                                                                                                          gCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGttt                                    <  1:402864/237‑1 (MQ=255)                                                                                                                                                                                                            gTTTCTTGATTAGCAAATTTGGCCTGGG‑GGTGATTTTGGTGTTTATGGGGATCCAGATGTTGATTGGCGACTGCTACCATATTCCAATCGACGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGtctgatc                                <  2:218842/239‑7 (MQ=255)                                                                                                                                                                                                              ttCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGGGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CCGCGGGGTGGGTAATTTTTAATCTGCCCAAGCCGTGTACCCTGTCATTACCACGAGCACCGTTTtctcc                               >  1:218842/1‑238 (MQ=255)                                                                                                                                                                                                                tctttttcttaaattgggCCGGGG‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTtctccctc                            <  2:619296/223‑1 (MQ=255)                                                                                                                                                                                                                 tcGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTtctccctct                           <  1:512186/239‑1 (MQ=255)                                                                                                                                                                                                                 ccgattgttaaaTTTGGCAGGGC‑GGTGATGCTGGTGTTTCACGGTAGCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTtctccctct                           <  1:31333/230‑1 (MQ=255)                                                                                                                                                                                                                   gATTCTTAAATTTGGCCTGGG‑GGTGATTTTGGTTTTTATCGGTATCAAGTTGCTGATTTGCGGCTTCTACCATATTCCAATCGCCGTCTCGCCGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTtctccctctcc                         <  2:346782/239‑1 (MQ=255)                                                                                                                                                                                                                       cTCAAATTTGGTCTGGG‑GGTGTTTCTGGTTTTTTTCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTc                     <  2:357520/239‑1 (MQ=255)                                                                                                                                                                                                                        tCAAATGTGGCCTGGG‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGGCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTcc                    <  1:839045/239‑1 (MQ=255)                                                                                                                                                                                                                        tCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGGGAATTATCGGCATGGG‑AA‑G‑CAGCGGGGTGGGTAATTTTTAATTTGCCTAAACCCGGTACCCTCTCATTAAACTATGCAACCTTTTTTCCCTCTCCCTTcc                    >  2:806987/1‑239 (MQ=255)                                                                                                                                                                                                                          aaaTATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTAccc                                                           <  1:418859/198‑1 (MQ=255)                                                                                                                                                                                                                          aaaTATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAg                  >  1:157693/1‑239 (MQ=255)                                                                                                                                                                                                                               tGGCCTGGG‑GGTGTTTTTGGTGTTTATCGGTATCAAGATGCTGGTTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGgaga             <  2:157693/239‑1 (MQ=255)                                                                                                                                                                                                                               tGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGgaga             <  1:806987/239‑1 (MQ=255)                                                                                                                                                                                                                               gggCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGgaga             <  1:667135/238‑1 (MQ=255)                                                                                                                                                                                                                                 gCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAAAATGAGCACCGGTTTCTCCCTTTCCCTTCCAGGGGGAgg           >  1:252084/1‑239 (MQ=255)                                                                                                                                                                                                                                      gC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGCCTTCTACCCTATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑AGGTTTATTA‑TCCCCGCCTGGGTGAATTATCGGCATGAT‑AA‑A‑CCGCGGGGTGGCTCATTTTTAATTTCCCTAACACGGGTACCCTCGCATTAACATGAGCCCCGATTTCTCCCTCTCCCTCTCACCGAGAGGGTCgg      >  2:174516/1‑239 (MQ=255)                                                                                                                                                                                                                                      gC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAAAATTAGAACCGTTTTTTCCCTCTCCCTTCCCGGGAGAGGGTCgg      >  1:751657/1‑239 (MQ=255)                                                                                                                                                                                                                                           tGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCgggggg  >  1:645317/1‑237 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                               TGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATTCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTATTTCCTGCTGGCGGGCGTAGCAGAGCGTTTCTCGATGCTCAAATATGGCCTGGC‑GGTGATTCTGGTGTTTATCGGTATCAAGATGCTGATTGTCGACTTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATG‑ACGTTTATTA‑TCAACGCCTGGGTGAATTATCGGCATGAT‑AA‑G‑CAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTG  >  NC_000913/3239156‑3239627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑