Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,293,685 T→C 17.6% A96A (GCT→GCC lpoA → OM lipoprotein stimulator of MrcA transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,293,6850TC17.6% 141.7 / 6.0 77A96A (GCT→GCClpoAOM lipoprotein stimulator of MrcA transpeptidase
Reads supporting (aligned to +/- strand):  ref base T (30/30);  new base C (6/9);  total (37/40)
Fisher's exact test for biased strand distribution p-value = 5.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTGGCACCCATACTCCCGATCAGTCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGA  >  NC_000913/3293476‑3293914
                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                        
tgtgGCCCCCATACTCCCGATCAGTCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGt                                                                                                                                                                                                                           <  1:683475/239‑1 (MQ=255)
 ttgccccccttccTCCCGATCAGTCCACTTCTTATATGCAGGGCACGGCCGCGGCTGATTCCTCCTTTTATCTTCCGCAGATGCAGCAAAGCTCTGATGATACCAGGAGCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTGGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTa                                                                                                                                                                                                                          <  2:482456/227‑1 (MQ=255)
 ttgGCACCCATGCTCCCGATCAGTCCACTGCGTATATGCAGGGCAAGGCGCAGGCTGATGCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTATGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTa                                                                                                                                                                                                                          <  1:873904/238‑1 (MQ=255)
 gtgggcccctttcTCCCGATTATTCCACTGCTTATAAGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTa                                                                                                                                                                                                                          <  2:274333/227‑1 (MQ=255)
 gtgGCACCCATACTCCCGATCAGGCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTa                                                                                                                                                                                                                          <  1:487691/239‑1 (MQ=255)
              tCCCGATCAGTCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTgg                                                                                                                                                                                                        <  1:155652/239‑1 (MQ=255)
                  gATCAGTCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTTAGTTTTTTAACCCACTACCGCAAAAACTGAACGAAGGCCAGCGTCGGGCAAAAAAACCTCTGGGCGGAAAGATTAAAACTGgcga                                                                                                                                                                                                    >  2:71772/1‑238 (MQ=255)
                          cACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGCTGTTTAACCAACTACCGCACGAACTTAACGATCGTCAGCGTCGCGAGCAAACACTGCTTGCCGTAGCGATTACACTGGTGCAGAAAGAtt                                                                                                                                                                                          >  1:125414/1‑239 (MQ=255)
                               cTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGGTGTTTAACCAAAAACCCCAAGCAATGAACGATGCCTAGCGTGCGGAGCAAAAACTGAGGGGGGAAGAGAGTTaaaacgaggacaaaaaatttatg                                                                                                                                                                                         >  2:676108/1‑219 (MQ=255)
                                  atatGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAGGGTAAAACCGGGCCGGCGGTTGTTTTGTTTCACCAACTATCCACAGAACTCAACAACGCTCGGCGCGGCGAGAAAAAACCTCTGGCGGGTGAGGTTACACAAgcgccctaaaatttttgtgta                                                                                                                                                                                   >  2:8438/1‑222 (MQ=255)
                                  atatGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGGGTTGTTTAACCACCTACCCCAAGAACTGAACAATGCTCAGCGTCGCGTGAAAACACTGCTGGCGGGAGAGATTAAACAGGCGCAGAAAAAttttttttg                                                                                                                                                                                   >  2:165562/1‑233 (MQ=255)
                                  atatGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAAAGTttgcttgc                                                                                                                                                                               >  1:676999/1‑239 (MQ=255)
                                       cAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGCGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCACCGTCCCGGGAAAACACTGCTGGCGGCAAGAATTAAAATGGGGCAAAAAGAATTTGTTGGGGCGCa                                                                                                                                                                         >  1:299326/1‑239 (MQ=255)
                                         gggCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGGGTTGTTTAAACAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGGAGGAGTTAAAACTGGGCGCACAGATTTTGCTGGCGCCCaaa                                                                                                                                                                       >  1:236451/1‑239 (MQ=255)
                                         gggCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCCACTACCGCAAGAACTGAACGATGCTCAACGTCGCGAGAAAACCATGCTGGCGGCGGGGGTTAAAATCGCGCGAAAAGATTTTGCTGGCGCGCcaa                                                                                                                                                                       >  1:28903/1‑239 (MQ=255)
                                         gggCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAAATACCGCAAAAACTGAACGATGCTCAGGGTCCGGGGAAAAAACTGCTTGGGGTAGAGATTTAAATGGGCCGCGAAAATTTTTCTGGGGCGCaaa                                                                                                                                                                       >  1:653413/1‑239 (MQ=255)
                                          ggCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCCAGAACTGAACGATGCCCAGCGTCGCGGGAAAAAACCCCTGGGGGCAGAGAATAAACTGGCGCAGAAAAACATTGCTGgcggcccaaa                                                                                                                                                                       >  2:61104/1‑232 (MQ=255)
                                          ggCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGGAAGAATTAAAATGGCGCGAAAAAATTTTGCTGGGCGCGCaa                                                                                                                                                                        >  1:685888/1‑238 (MQ=255)
                                          ggCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCCCGCGAAAACACTGCTGGCGGGAGAGGTTTAACTTGCGCCGAAAAGTTTTGCTGTGCGCCCaaa                                                                                                                                                                       >  1:72841/1‑239 (MQ=255)
                                              cGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCCAGAACTGAACGATGCTCAGCGTCGCGAGGAAACACTGCTGGCGGTGAGGGTTAAACTGGCGCCGAAAGATTTTTGTTGAGCGCCAAACTTg                                                                                                                                                                  >  1:711115/1‑239 (MQ=255)
                                               ggCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCCCAGCGTCGCGCGAAAAAACAGCTGGCGGTAGAGATTTAAACTGCGACAAAAAAATTTTCTGGGGCGCAAAACttcc                                                                                                                                                                 >  2:375590/1‑237 (MQ=255)
                                                 cgcAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGGTCAGCGTCGCGAGAAAACACCGCGGGCGGGAGAGAGTAAAAATGCGCGGAAAAATATTTTCTGGGCGCaaaaaatggtg                                                                                                                                                               >  2:706230/1‑232 (MQ=255)
                                                           ttCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTTGTTTACCAACTACCGCCAAAACTGAACCATGCCCACCGGCCGCGGAAAACACTGCCGGCGGAACGGCATAAAACGTCGCCCAAAAGATTTTGTGGCCCGCGAAAATTGCTGGGGacaaaaa                                                                                                                                                     >  2:398300/1‑236 (MQ=255)
                                                           ttCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAAATGGACGATGCTCTGCGGCGCGGGAAAACACTGCTTGCGGGAGGAATTTAAATGGGGCAGAAAGATGTTTCTGGGCGCCAGAACCTGCTGGCGGAAAAca                                                                                                                                                     >  2:139823/1‑239 (MQ=255)
                                                                ccTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGGGAAAACAATGCTGGCGGTAGGGATTAAACTGGCGCCACAAGATTTTGCTTGCGCGCAAAAATTTCTTGCGcaaaaccaacc                                                                                                                                                 >  1:846378/1‑231 (MQ=255)
                                                                ccTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGCCGGTTGAGTTGTTTAACCAACTACCCGCAGAACCGAACGATGCTCAACGGCGCGAGAAAAAACTGCTGGCGGTGAGAGTTGAACTGGCGCCGAAAAATTTTTCGTGGCCGGCAAAATTGCTGAGGaaaaaaaaaac                                                                                                                                                 >  2:74994/1‑231 (MQ=255)
                                                                 cTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAAATACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTCGCGGTAGAGGTTAAACAGGCGCAGAAAAAATTTGCTGGGCGGCAAAACATGCTGGGCAAAATcaacact                                                                                                                                                >  1:769897/1‑233 (MQ=255)
                                                                  ttttATTTTCAGCAGATGCAACAAGGTTTTGATGATACCAGGAACAACGGGCAATTCCCCGCCATTCGTGCACTGGGAAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCGGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGc                                                                                                                                              <  2:7938/239‑1 (MQ=255)
                                                                            cggagATGCAACAAAGTTTTGATGATCCCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGaa                                                                                                                                  <  1:568320/236‑1 (MQ=255)
                                                                                 aTGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAACGAAGGTAAAACCGGGCAGGCGGTTTAGTTTTTTAACCAGATACCCCAAGAAATTAACGATGCCCAACCTCGGCGAAAAACCCTGCGCGCGGGGGAGAATACAACGGGCGCAAAACGTATTTCTGGGCGGCCAAAACTACTGGGCGAAA‑‑CACACCCACCGCATTTAGAaaaaaa                                                                                                                             >  2:330262/1‑239 (MQ=255)
                                                                                           gCTCTGATGATACCAGGTACAATTGGCAATTGCTCGTCATTTGTGAACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGGTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAgcg                                                                                                                   <  2:192851/239‑1 (MQ=255)
                                                                                            ctctGATGATGCCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGAttt                                                                                                                                      <  1:611380/219‑1 (MQ=255)
                                                                                            ctctGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGGAGAGATTAAACTGGGGCAGAAAAATTTTGCTGGGGCGCAAAACTTGCTGGCGAAAATTACACCTGCCGATTTAGAACAAAACCACCaaaccc                                                                                                                  >  1:804232/1‑235 (MQ=255)
                                                                                                    atACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAAATACCGCGAGAACTGAAAGATGCTCAGCGTCCCGAGAAAACACTGCTGGCGGGAGAGAGTCAACAGGCCCAGAAAGATTTTTGTGTCGCGCGAAACTTGACTGCGGAAAACACCACAGCCGCTTTTGAACAAAAacaacacaggcggtaattc                                                                                                          >  2:624357/1‑226 (MQ=255)
                                                                                                     tcccAGGATCAGCGGGCAATTACTCGACATTCGTGCAATGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTGGAACAAAACCAGCAAGCGCGTTACTgg                                                                                                          <  1:180550/236‑1 (MQ=255)
                                                                                                     tcccAGGATCAACTGGCAATTACGCGCCATTCGTGCACTGGGGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTgg                                                                                                          <  1:165562/236‑1 (MQ=255)
                                                                                                      aCCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCCGCGTCGCGAGAAAACAATGCTGGCGGGAGAGATTAAAATGGCGGCGCACAAATTTTTCTGGGCGCCAAACCTTCGTGCGGACAACACCACCGCCCATTTTGAAAAAAAACAAACAGCGCGTTAATggca                                                                                                       >  2:919038/1‑239 (MQ=255)
                                                                                                           gATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAAATACCGCAAGAACTGAACGAGGCTCAGCGTCGCGAGAAAACACTGCTGGCGGGAGAGGATAAAAATGCGCCGGAAGAATTTTCTGGGCGGCCCAACTTTCTGGGGAAAAACAACCCTCCGATTTTAAAAAAAAACAGCaaaccgcgtaacagcagagaaa                                                                                                  >  2:627783/1‑219 (MQ=255)
                                                                                                                aCTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGCTGCTCAGCGTCGCGGGAAAACACTGCCGGCGGTAAAGATTAAACGGGGGCAGAAAACATTTGCTGGGGCGCAAAACTTGCCGGCGAAAATAACACCCGCCGATTTAGAACAAAAAAAACAAGCACGTTAACGGAAGGCaaaaaaa                                                                                            >  2:259804/1‑237 (MQ=255)
                                                                                                                aCTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGGTTAAACTGGCGCAGAAAAATTTTGCTGGCGCGCAAAACTTGCTGGCGGAAATTACACCTTCCGCTTTAGAACAAAACCAGCAAGCGCGTTACTGGCCAGCCaaaaac                                                                                             >  1:717734/1‑238 (MQ=255)
                                                                                                                    gCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAAATTTTTCTGGCGCGCAAAACTTGCTGGGCAAAATCAAACCTTCCCAATTTGAACAAAAACAGCAAGCCGGTTAATTGCAAGCcaaaaaagct                                                                                         >  1:280560/1‑233 (MQ=255)
                                                                                                                    gCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCGCAGCGTCGCGAGAAAACAACGCTGGCGGGAGAGAGTAAAATGGGGCGGAAAGATTTTTCTGGCGCGCCAAACATTGTGGGGAAAATAACACACGCCGGATTTGAAAAAAAACCAACAAGCGCGTTAATGGAGGGaaaaaaaccag                                                                                         >  2:708801/1‑234 (MQ=255)
                                                                                                                     caatgcccggCCATTGGTGCCGTGGTAAAAAAAGGAAAGACGGGGCGGGGCGTGAGTTTGTTAAACCAAGTGCCGGAAAGACGTAACGAAGCCAAGCGTGCCGAGAAACCACTGCTGGCGGTAGAGATTAAACGGGCGCAGAAAGAGTTTGCGGGCGCGCAAACCTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATg                                                                                        <  2:28903/229‑1 (MQ=255)
                                                                                                                     cAATTCCCCCCCTTTCGTGCCTTGGTGAAAGAAGGTAAACCCGGGGTGGGGGTTGTGTTGTTTAACCAACTTGCGCAAGAACTGACCGATGCTCAGCGTCGCGAGAAAACCCTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGAAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATg                                                                                        <  2:676999/238‑1 (MQ=255)
                                                                                                                     cAATTACCCGCCATTCGTGCACTGGGGAAAGAAGGGAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAACGCAAGAACTGAACGATGCGCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATg                                                                                        <  1:919038/238‑1 (MQ=255)
                                                                                                                     aattttcctgCCTTTCGGGCCCTGGTAAAAAAAGGTAAAACGGGGAAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACGGGCGCAGAAAGATTTTGCGGGCGCGAAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATg                                                                                        <  2:213340/229‑1 (MQ=255)
                                                                                                                                ggttCGTGCGCTGGTGAAAAAAGGAAAAACCGGGAAGGCGGTTGAGTTGTGTAACCAACTACCGCAAGAACTGAACGATGCCCGGGGCGGAGAGAAAGCACGGCCGGCGGTCGGGATTAAACAGGCGCAGAAAGATTTTGCGGGCGCGCAAAACTTGCTGGAGAAAATCACCCCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAgggg                                                                            <  2:404120/236‑1 (MQ=255)
                                                                                                                                cATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAgggg                                                                            <  1:71772/238‑1 (MQ=255)
                                                                                                                                 attggggCACTGGTGAAAGAAGGTAAACCCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCg                                                                          <  1:51163/233‑1 (MQ=255)
                                                                                                                                         cttgggaaaaGAAGGTAAAACCGGGCAGGCGTTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTAAGGCTCGCGAAAAAACGCTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTACTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCa                                                                  <  1:38524/232‑1 (MQ=255)
                                                                                                                                         attGGGGAAAAAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGAAAGAACTGAACGATGCCCAGCGTAGCGAGAAAACCCTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGAAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCa                                                                  <  1:375590/237‑1 (MQ=255)
                                                                                                                                         aCTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCa                                                                  <  1:61104/239‑1 (MQ=255)
                                                                                                                                         aCTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCa                                                                  <  1:781305/239‑1 (MQ=255)
                                                                                                                                         aCTGGGAAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACGGAACGATGCCCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAAATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCa                                                                  <  1:398300/239‑1 (MQ=255)
                                                                                                                                          ctgggtaaaGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCa                                                                  <  1:376044/232‑1 (MQ=255)
                                                                                                                                           atgTGAAAGGAAGGTAAACCCGGGAAGGCGGTTGGGTTGTTTAACCAACTACCGAAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCa                                                                  <  1:8438/236‑1 (MQ=255)
                                                                                                                                             gTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGGGCAGAAAAAATTTGCTTGCGCGCAAAACTTGCTGGCGAAAATCACAACTGCCGATTTAGAACAAAACCAGCAAGCGCGGTACTGGCAGGCAAAAATCGATGCCAGCCACGGGCCGCCTTCCCtt                                                                >  1:765266/1‑237 (MQ=255)
                                                                                                                                                  agaagGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGGAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGGAAATCACACCTGCCGATTTAGAAAAAAACCAGCAAACCCCTTACTGGCCGGCCAAAAATCATGCCCGCCCAGGGCGGCCCTTCCATGAtttta                                                         >  1:191072/1‑237 (MQ=255)
                                                                                                                                                     aagGAAAACCCGGGAGGGGGTTTGAGTTTTTTAACCAACTACCGAAAAAATTGACGGAGGTCCAGGGTCGCGGGAAACCCCTCGTGGGGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGGGAAAATCCCCCCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTg                                                       <  2:685888/239‑1 (MQ=255)
                                                                                                                                                        gTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGGAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAACGCGTTACTGGCAGGCAAAAAACGAAGCCAGCCAAGGGGGTCCCTCCCTTGTTTTACTgcgc                                                    >  1:12364/1‑239 (MQ=255)
                                                                                                                                                         tAAACCCGGGCAGGGGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTgcg                                                     <  1:706230/237‑1 (MQ=255)
                                                                                                                                                         tAAACCCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTgcg                                                     <  1:754065/237‑1 (MQ=255)
                                                                                                                                                         tAAAACCGGGCAGGCGGTTGAGTTTTTTAACCAACTACCGCAAGAACTGAACGATGGTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTgcg                                                     <  1:853582/237‑1 (MQ=255)
                                                                                                                                                         aaaaaCCGGGGGGGGGTTTGATTTTGTTACCCAACCACGGAAAAAACGGAAGGAGGTCAAGGGTCGCGATAAAACCCTGCGGGCGTGAGGGATTAAACGGGCGCAGAAAGATTTTGCGGGCTGACAAAACTGGCTGGCGAAAATCACCCCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTgcg                                                     <  2:236451/236‑1 (MQ=255)
                                                                                                                                                                gggcgggcGTTTGATTTTTTTAACCAATTACCGCAAGAACTGAACGAGTCCCAGGGGCGCGAAAAAACACGGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACCCCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAAt                                           <  2:12364/239‑1 (MQ=255)
                                                                                                                                                                gggCAGGTGTTTTAGTTGTTTAACCAATTACGGAAAGAACTGAAAGAGGCCCAGAGCCCGGAGAAAACGCCGCTGGAGGTAAGGTTTAAACTGGCGCAGAAAAGTTTTGGCGGCCCGAAAAACTTGCTGGCAAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAAt                                           <  2:254283/239‑1 (MQ=255)
                                                                                                                                                                        ggTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCCGAAAGATTTTGCTGGCGCGCAAAAACTGCTGGCGAAAATCACACCTGCCGATTTATAACAAAAACAGCAAAAGCGCTACTGGCAGGCAAAAATAGATGCCCCACCCGGGCGGCCCTCCATTTGTTTTCTTCGCGCGGTTAAtttccagg                                    >  2:667877/1‑233 (MQ=255)
                                                                                                                                                                                   tttAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGGTAATTGCCCAGGAACCgctgct                         >  1:155967/1‑239 (MQ=255)
                                                                                                                                                                                         cAACTAACGCAAGAACTGAACGGTTGTCAGCGTCGGGAGAAAACACTGGTGGCGGTAGAAATTAACCTGGCGCAGAAAGATTTTGCTGGCGCGAAAAACTTGCTGGAGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGgcgc                   <  1:430299/239‑1 (MQ=255)
                                                                                                                                                                                             tACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCCAAAAACCATGCCCGCCAGGGGCGGCCTTCCCTTATGTTACTGCGCCCGTTAATTGCTCACGCACCGCTGCCTGGCGCgaaa               >  2:141049/1‑239 (MQ=255)
                                                                                                                                                                                             tACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCCGCTTAATTGCTCCGGAACCGCTGCTTGGCGCgaaa               >  1:182826/1‑239 (MQ=255)
                                                                                                                                                                                                   aaGAACTGAACGATGCTCAGCGTCGCGAGAAACCCCTGCGGGCGGTAGAGATTAAACTGGCGCAAAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCAAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGaaaaa         <  1:679685/239‑1 (MQ=255)
                                                                                                                                                                                                      aaCTGAACGATGCTCAGCGTCGCGAGAAAACCCGGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAAcag      <  1:809938/239‑1 (MQ=255)
                                                                                                                                                                                                       aCTGAACGATGCTCAGCGTCGCGAGAAAACCCTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGTTTTTGCGGGCGCCCAAAACTGGGTGGAAAAAACCACCCCTGACGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAAcag      <  2:72841/238‑1 (MQ=255)
                                                                                                                                                                                                       aCTGAACGATGCCCAGCGTCGCGAGAAAACCCTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGGAAAACTTGCTGGCGAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAAcagca    <  1:627783/239‑1 (MQ=255)
                                                                                                                                                                                                         ttaaCGAGGCTCAGGGTCGCGAGAAAACACTGCGGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCCCGAAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAAcagcag   <  1:100366/237‑1 (MQ=255)
                                                                                                                                                                                                          gAACGATCTTCAGCGGCGCGAGAAAACACGGGCGGCGGTGAAAATAAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCGGGCGAAAATCCCACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGa  <  2:711115/239‑1 (MQ=255)
                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                        
TGTGGCACCCATACTCCCGATCAGTCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGA  >  NC_000913/3293476‑3293914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: