| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,403,735 | T→C | 7.3% | L85P (CTC→CCC) | acuI → | putative acryloyl‑CoA reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,403,735 | 0 | T | C | 7.3% | 223.4 / 2.2 | 78 | L85P (CTC→CCC) | acuI | putative acryloyl‑CoA reductase |
| Reads supporting (aligned to +/- strand): ref base T (32/39); new base C (3/4); total (35/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
CAGGACGGCAAAACTCTCGCATCAGTACAGACTCTGGACGAAAGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAA‑GATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTACCGCCGTG > NC_000913/3403506‑3403973 | cagGACGGCAAAACTCTCGCATCAGTACAGACTCTGGACGAAAGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAA‑GATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGGGTTACTCCctggctg > 1:501199/1‑239 (MQ=255) tgcaggtgtgtataaaggaagtctctGGAGGAAAGTCGCTCGCCGGAGGGCGATGTCACGGTCGATGTTCCCTGGGCGGTCCAGGACTATAAA‑GATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGt < 2:238634/214‑1 (MQ=255) aaaCTCTCCCCTCAGTACCGACTCTGGACGAAAGTCGCCTGCCGGAGGGCGATTTCACGGTCGATGTTCACTGCGCGAGCCTGAACTATAAA‑GATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCCTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGt < 1:906875/239‑1 (MQ=255) cgctcGCATCCGTACAGACTCTGGGCGAAAGTCGCCTGCCGGATGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAA‑GATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGaa < 1:283272/237‑1 (MQ=255) cctccgtaTCAGTACAGGCTTGGGACGAAATTCGCCTGCTGGAGGGAGATGGCACGGTCGATGTTCAATGGTCGAGCCTGAACTATAAA‑GATGCGCTGGTGATTCCCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGaa < 2:448002/232‑1 (MQ=255) gCATCAGTACAGACTCTGGACGAAAGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAA‑GATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAAcc > 1:853841/1‑238 (MQ=255) ccctccgtccAGACTTCGGAAGAAAGGCGCCTGCCGGGGGGCGATGTCACGGTCTATGTTGACTGGTCGAGCCTGAACTATAAA‑GATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCa < 2:772423/230‑1 (MQ=255) ttttgggttttgtgggAAAGTCGCCTGGCGGAGGGCGGTTTTACGGTCGGTGGTCACTGGGCGAGCCTGAACTATAAA‑GATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTgggg < 2:347170/224‑1 (MQ=255) tACAAACTCTGGAAGGAAGTCCGCTGCCGGGGGGCGATGGCACGGTCGATGTTCACGGGTCGAGCCTGAACTATAAA‑GATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTGCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCGCCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTgggg < 2:501199/238‑1 (MQ=255) cgACTTTGGAAGAAAGTCGCCTGCGGGGGGGGGCTGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAA‑GATGCGCTGGCGATTACCGGTAAGGGAAAAACCATCCGTAATTTGCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTggg < 2:369426/238‑1 (MQ=255) aCTCTGGACGAAAGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAA‑GATGCGCTGGCGATTCCCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATTCCGGTCAGGAGGTGTTACTCACTGCCTGGGGGGTTGGGGAAAACCACTGGGGTgg > 1:122924/1‑236 (MQ=255) aCTCTGGACGAAAGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAA‑GATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGCACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCCGGCGGCGTTACTCACTGGCTGGGGCGTTGGTGAAAACCAATTGGGTgg > 1:886016/1‑236 (MQ=255) aCTCTGGACGAAAGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAA‑GATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGGGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTgg > 1:750979/1‑236 (MQ=255) ccttGGACGAAAGTGGCCTGCCGGAGGGCGATGGCACGGTCGATTTTCACTGGTCGGGCCTGAACTATAAA‑GATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAAGTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTggg < 2:853841/233‑1 (MQ=255) tctGGACGAAAGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAA‑GATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGaa < 1:846817/220‑1 (MQ=255) tGGACGAAAGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAA‑GATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGGGTTACCCACTGGCTGGGGCGTTGGTGAAAACCCCTGGGGGGGGCggggg > 2:625968/1‑237 (MQ=255) cggACGAAAGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAA‑GATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGGAAACCACTGGGGGGGGCTGGCg > 1:903197/2‑239 (MQ=255) ggACGAAAGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAA‑GATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCGGGTGGTGTTACCCACGGGATGGGGCGGTGTGGAAAACCACTGGGGGGGGATGGCgg > 1:379046/1‑239 (MQ=255) ggACGAAAGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAA‑GATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGGGGGGTTACTCACTGGCTGGGGCGTTGGGGAAAACCACGggggggggggggggg > 2:718957/1‑232 (MQ=255) ggACGAAAGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAA‑GATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTc < 2:94842/196‑1 (MQ=255) ggACGAAAGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAA‑GATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTc > 1:94842/1‑196 (MQ=255) ggACGAAAGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAA‑GATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGGGTTACTCCCTGGCTGGGGGGGTGGGGAAAACCCCTGGGGGGGGGcggcgg > 2:549312/1‑239 (MQ=255) aGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAA‑GATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTTCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATCCCGGTAGGGGGGTGTTACTCACTGGCTGGGGCGGTGGTGAAACACCCTgggggtgtctctttaccacatc > 2:846817/1‑221 (MQ=255) aGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAA‑GATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCCGGAGGTGTTACTCACTGACTGGGGCGTTGGCGAAAACCACTGGGGTGGGCTGCCCGAGCCGgc > 1:84147/1‑239 (MQ=255) aGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAA‑GATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCAGGGGCGTTGTTGACAAc > 1:238634/1‑213 (MQ=255) cGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAA‑GATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGaa < 1:56617/238‑1 (MQ=255) aTGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAA‑GATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGACCCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGCGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGGGActgg > 1:206704/1‑239 (MQ=255) aTGTTCACTGGCCGAGCCTTAACTATAAA‑GATGCGCTGGCGATTGCCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCAt < 1:411532/238‑1 (MQ=255) cACGGGGCGAGCCTGGACTATAAAGGGTACG‑TGGCGAGTCCCGGTAGGGGAAAAATCATCCGTAATTTTCCGATGATTCCTTGGATCGATGTTGGCGGAACTG‑TACGCTCCAGCGAAGATCCGCGTTTTCATGCCGGTCGGGAGGGGTTACTCACTGGCTGGGGCGTTGGGGAAACCCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCa < 2:404622/239‑1 (MQ=255) cgtggtcggacctgatatgaaaa‑TATGCGCGGGCGTTTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGTGTTTGCCGGAACTT‑GACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCa < 2:886016/218‑1 (MQ=255) tCGAGCCTGAACTATAAA‑GATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCa > 2:622296/1‑173 (MQ=255) tCGAGCCTGAACTATAAA‑GATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCa < 1:622296/173‑1 (MQ=255) tCGAGCCTGAACTATAAA‑GATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGGGACGGCGGGGGGGGAAGGGGACGGGCTGGGTGCCATCCCCCAAggggg > 2:176759/1‑237 (MQ=255) cgcTGAACTTAAAA‑GGTGCGCTGGCAATTACCGGTAAGGGAAAAATCCTCCGTAATTTTCCGATGATTCCTGGGATCGATTGTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGAc < 1:23333/237‑1 (MQ=255) ttaAA‑GATGCGCTTGGGATTCCCGGTAAGGG‑AAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAg < 1:710953/238‑1 (MQ=255) taAA‑GATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCActggctg > 2:439038/1‑141 (MQ=255) taAA‑GATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCActggctg < 1:439038/141‑1 (MQ=255) taAA‑GATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGGGAGTGAAAGGTGACTGGCCGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAg > 1:400709/1‑239 (MQ=255) taAA‑GATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAg < 1:608410/239‑1 (MQ=255) aggttgtgctgtggtttcccGGTAAGGGAAAAATACTCCGTAATTTCCCGATGATGCCTGGGATCGATTTTGCCGGACCTG‑GACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCa < 2:122924/221‑1 (MQ=255) aTGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGGGAAAGGTGACTGGCTGGTTGACATGCCGCAAGGGCTGGACGAGGGCAAAGCAATg > 1:139755/1‑239 (MQ=255) agcactggggtgtACCGGTAAGGGAAAAATCACCCGTAATTTTCCGATGATTCCGGGGATCGATTTTGCCGGAACTG‑GACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTGACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATg < 1:568399/226‑1 (MQ=255) cgcTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGAtt > 1:654604/1‑239 (MQ=255) cgcTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGGGAAAGGGGACTGGCTGGTTGCCCTGCCGCAAGGGCTGGAGCCGCGGAAAGCAAGGAtt > 1:47753/1‑239 (MQ=255) tGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTAt < 1:549312/238‑1 (MQ=255) gggttACCGGTAAGGGAAAAATTCTCCGTAATTTTCCGTTTATTCCTGGGATCGATTTTGCCGGAACGT‑TACGCCCCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCgg < 2:287868/235‑1 (MQ=255) ctaTTACCGGTAAGGGAAAAATCATCCGTAAGTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGAACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCgg < 1:176759/236‑1 (MQ=255) ccaTTACCGGTAAGGGAAAAATCATCCGTAATTTCCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCgg < 1:291490/236‑1 (MQ=255) ccGGAAAGGGAAAAATCCCCCGCTATTTCCCGATTATTCCTGGGGTCGTTTTGGCCGGACCTG‑TACGCCCCGGCGAAGATCCGCGTTTTCATGCCGGTCGGGAGGTTTTACTCACTGGCTGGGGCGTTGTTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGcc < 2:654604/239‑1 (MQ=255) gTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGAAGCGGGTAAGGCAATGATAATCGGTAATGCCGGt > 1:681441/1‑239 (MQ=255) gAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCCCCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTa < 1:625968/237‑1 (MQ=255) aaaaaaTCATCCGTAATTTTCCGATGATTCCTGGTATCGATTTTGCCGGAACTG‑TTCGGACCAGCGAAGATCCGCGTTTTCAGGCCGGTCAGGAGGTGTTACCCACTGGCTGGGGCGTTGGTGAAACCCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTa < 1:915018/236‑1 (MQ=255) aaaTCCTCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGTTCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGcc < 1:718957/239‑1 (MQ=255) aaaTCATCCTTATTTTTCCGTTTTTTCCTGGGTTCGTTTTTGCCGAGACGG‑TCCGCCCCGGGGAAGTTCCGGGTTTTCATGCCGGTCAGGAGTTGTTACTCCCTGGTGGGGGCGTTGGTGAAAACCCTTGGGGGGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCAGGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGcc < 2:47753/239‑1 (MQ=255) aaTCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCa < 2:786731/186‑1 (MQ=255) aaTCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCa > 1:786731/1‑186 (MQ=255) tcCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGGGGGAAAGGGAACCGGCGGGTTGCCATGCCCCCAGGGGTGGGCCCGCGCAAAAGAATGATTTTTGGTCATGCCGGGTTTTACGCCCTgggg > 2:896904/1‑236 (MQ=255) ttcccgtttgttcctgggctggctttGGCGGGACTGTTACGCACCAGCGAAGATCCGGTTTTTAATGCCGGTCAGGGGGTGTTACTCACTGGCTGGGGGGTTGGGGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGGGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGAAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGAt < 2:400709/216‑1 (MQ=255) tCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCCTGCTGTTCGGGATGGCGCTGGAAg > 1:15064/1‑239 (MQ=255) ggggagtG‑TCCGCACAGGGGAATATCCGGGTTTTCAGGCCGGTTGGGGGGTGTTACCCCCGGGGTGGGGCGTGGGTGAAACCCCCGGGGGTGGGCTGGCGGGGCAGGCGCGGGTGAAAGGTGCCGGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCg < 2:522080/232‑1 (MQ=255) cGGAACTG‑TCCGCCCCAGGGAAGTTCCGCTTTTTCATGCCGGTCAGGAGGTGTTACTCGCTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGc < 1:135795/239‑1 (MQ=255) tACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGGGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACggg > 1:522080/1‑239 (MQ=255) aCGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACCCACTGGCTGGGGCGTTGGTGAAGACCACTGGGGTGGGCTgg > 2:351026/1‑89 (MQ=255) aCGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACCCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTgg < 1:351026/89‑1 (MQ=255) ccaagggAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTCCTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGgaga < 1:531293/232‑1 (MQ=255) ttCCGGGTTTGCAGGCCGGTCAGGGGGTTTTACCCCCTGGCGGGGGGGTTGGGGAAACCCGATGGGGTGGGCTGGCGGAGCAGGCGCGAGTTAAAGGTGCCTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACg < 1:135527/238‑1 (MQ=255) cgTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGGGATTGTCGTGACGGGGGc > 1:395625/1‑239 (MQ=255) tgtTACTCACTGGCTGGGGCGTTGGTGAAAACCACTgtgg < 1:847321/40‑1 (MQ=255) tgtTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTa < 1:705221/141‑1 (MQ=255) gtTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGAGGGGGAGATTGTCGTGACGGGTGCCAGGGGTGGCGTCGGCCGTAcc > 1:882867/1‑238 (MQ=255) ttACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGAc < 2:452368/102‑1 (MQ=255) ttACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGAc > 1:452368/1‑102 (MQ=255) ttACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGAtt < 2:656077/120‑1 (MQ=255) ttACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGAtt > 1:656077/1‑120 (MQ=255) ttACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGcc < 1:848035/132‑1 (MQ=255) ttACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGAAATGGTTATCGGTACTGcc > 2:848035/1‑132 (MQ=255) aCTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCCGGACGGGTGCCCGGGGGGGGGTCGTCAGGAccggcg > 1:687793/1‑239 (MQ=255) aCTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGGGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGGAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGGGATGGCGCTGGAAGATGCCGGGGTTCGCCCGCCGGCCGGGGGGGTTGGCGGGACGGGGGCCAGggggggggcgggcgggacgcccg > 2:813132/1‑222 (MQ=255) tCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGGGATTGTCGTGACGGGTGCCAGTGGGGGCGTCGGCAGTACCGCCGTg > 1:909480/1‑239 (MQ=255) | CAGGACGGCAAAACTCTCGCATCAGTACAGACTCTGGACGAAAGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAA‑GATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTG‑TACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTACCGCCGTG > NC_000913/3403506‑3403973 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |