Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,414,308	T→C	18.0%	A149A (GCT→GCC)	acrE →	cytoplasmic membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,414,308	0	T	C	18.0%	204.4 / 5.2	96	A149A (GCT→GCC)	acrE	cytoplasmic membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (36/39);  new base C (14/5);  total (51/45)
Fisher's exact test for biased strand distribution p-value = 7.02e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGATCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTA‑CCAGAT‑CGATCCCGCGACCTATCAGGCAAATTATGACAGCGCGAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGA‑CTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGA‑CCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCG  >  NC_000913/3414079‑3414546                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                 ggATCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTA‑CCAGAT‑CGATCCCGCGACCTATCAGGCAAATTATGACAGCGCGAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGCAAT‑ACATCAGCCAGCAGGAGTCCGACCAGGCCATTGCTGATGCCCGTCCGGcc                                                                                                                                                                                                                                         >  2:867484/1‑239 (MQ=255)    tCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTA‑CCAGAT‑CGATCCCGCGACCTATCAGGCAAATTATGACAGCGCGAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGGTACGTTCCGCTCGTGGG‑AACGAAAT‑ACATCACCCAGCCGGCGACAGACCAGGCCATTGCTGGTGCCCGTCCAGCCgc                                                                                                                                                                                                                                       >  2:49618/1‑237 (MQ=255)            aaTCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTA‑CCAGAT‑CGATCCCGCGACCTATCAGGCAAATTATGACAGCGCGAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGATTACGACCAGGCCATTGCTGATGCACGTCAGGCCGATGccgccgg                                                                                                                                                                                                                              >  1:767536/1‑238 (MQ=255)            aaTCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTA‑CCAGAT‑CGATCCCGCGACCTATCAGGCAAATTATGACAGCGCGAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCCCGTCAGGCCGATGccgccgg                                                                                                                                                                                                                              >  1:188893/1‑238 (MQ=255)            aaTCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTA‑CCAGAT‑CGATCCCGCGACCTATCAGGCAAATTATGACAGCGCGAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCCCGTCACGCCGATGCCGCCGt                                                                                                                                                                                                                              >  1:764434/1‑239 (MQ=255)                 cAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTA‑CCAGAT‑CGATCCCGCGACCTATCAGGCAAATTATGACAGCGCGAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TCCGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGTCAGTGCCCGCCAGGCCGaaccccccgggagtc                                                                                                                                                                                                                         >  2:167321/1‑225 (MQ=255)                 cAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTA‑CCAGAT‑CGATCCCGCGACCTATCAGGCAAATTATGACAGCGCGAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGGGTACGACCAGGCCATTGCTGATGCCCGGACGGCCGATGCCCCCGGGagt                                                                                                                                                                                                                          >  2:525615/1‑236 (MQ=255)                      tcccgtggggCAGGGGTGTGAAAGCAGGCCGGCCCCTGAA‑CCAGAG‑CGATCCGGCGACCTATAAGGAAAATTATGACAGCGCGAAAGGCGAACTGGCG‑AAAAGGAAAG‑CCGCCGCCGCAATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TAAGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCa                                                                                                                                                                                                                    <  2:838174/231‑1 (MQ=255)                      tcccTGAAGGCGGAGATGTCGAAG‑ACGCCAGGCACTGTACCCAGAC‑CGACCCCCGGGCCTAGAAGGAAAATTCAGCCAGCGCGAAAGGCGAATTGGCG‑AAAATGGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCa                                                                                                                                                                                                                    <  2:767536/236‑1 (MQ=255)                       cccggAAGGCGGCGGTGTCCAAGGAGGCCGGGCCCTTTA‑CCAAGA‑CGACCCCGGGACCTAACAGGAAAATTAGGCCGAGGGGAAAGGCGAACGGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCaa                                                                                                                                                                                                                   <  2:106040/235‑1 (MQ=255)                          ggAAGGCAGGGATGGGAAAGCAGGCCAGTCCCTGTA‑CCAGAC‑CGATCCCGCGACCTATCAGGCAAATTAGGACAGCGCGAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAg                                                                                                                                                                                                                <  1:86530/238‑1 (MQ=255)                             aGGCAGCGAGTGGAAAGCAGGCCAGGCCCTGGA‑CCAGAT‑CGATCCCGCGACCTATCAGGCAAATTATGACAGCGCGAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCca                                                                                                                                                                                                             <  1:812615/239‑1 (MQ=255)                                   cGATGTGCAAGCAGGCCAGTCCCTGTA‑CCAGAT‑CGATCCCGCGACCTATCAGGCAAATTATGACAGCGCGAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACGTCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATGTCCGCAAAACCCACAGTCg                                                                                                                                                                                                       >  1:37535/1‑239 (MQ=255)                                           aaccAGGCCAGTCCCGGTA‑CCAGAT‑CGATCCCGGGCCCGAGCAGGAAAATGATGACAGCGCAAAAGGCGAACGGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCt                                                                                                                                                                                               <  1:66045/236‑1 (MQ=255)                                           aaGGAGGCCGGTCCCGGGC‑CCGGAT‑CGGTCCCGGGACCTAACAGGAAAATTAGTGCAGGGCG‑AAGGGGAAATGGGGAAAAAGTGAAG‑CCGCCGCCGCCACCGCGAATTTGAC‑GGTAAAACGTTACGTTCCGCCCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCt                                                                                                                                                                                               <  2:852227/239‑1 (MQ=255)                                                  ccAGTCCCTGGA‑CCAGAT‑CGATCCCGCGACCTATCAGGCAAATTATGACAGCGCGAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCAt                                                                                                                                                                                          <  1:470488/237‑1 (MQ=255)                                                       cccgtga‑CCAGAC‑CGACCCCGCGGACGATCAGGAAAATTAGGACAGCCGGAGAGGAGAACTGGCA‑AAAAGTGAAC‑CCGGCGCGGCCATCGCTCATTGGAC‑GGAAAAACGTTACGTTCCGCTCGGGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGCCCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATc                                                                                                                                                                                     <  2:824865/231‑1 (MQ=255)                                                       cccTGTA‑CCAGAT‑CGATCCCGCGACCTAGCAGGCAAATTATGACAGCGCGAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAAt                                                                                                                                                                                      <  1:167321/236‑1 (MQ=255)                                                        ccTGTA‑CCAGATCCGTACCCCGGACCTAGCCGGCAATTGAAGCCGGAGCGAAAGGAGAACGGGGA‑AAAAGTAAAC‑CGGCCGCCGCCATCGCGCATTGGAC‑GGTAAAGCGTTACGTTCCGCCCGTGGT‑TACGAAAT‑ACCTAAGCCAGCGGGAGTACGACCAGGCCATTGCTGATGCGCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAAt                                                                                                                                                                                      <  2:487549/236‑1 (MQ=255)                                                           gTA‑CCAGAT‑CGATCCCGGGACCTATCAGGCAAATTATGACAGCGCGAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCtt                                                                                                                                                                               <  1:547874/239‑1 (MQ=255)                                                           gTA‑CCAGAT‑CGATCCCGCGACCTATCAGGCAAATTATGACAGCGCGAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCCCGTCAGGCCGATGACGCCGGGATTGCCGCAAAAGACACAGTCGCAAGCGCTCGCATCAATCTTGCtt                                                                                                                                                                               >  1:373451/1‑239 (MQ=255)                                                           gTA‑CCAGAT‑CGATCCCGCGACCTAACAGGCAAATTATGCCAGCGCGAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCtt                                                                                                                                                                               <  1:264526/239‑1 (MQ=255)                                                              cccAGGT‑CGATCCCGCGACCTATCAGGCAAATTATGCCAGCGCGAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTGGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTtat                                                                                                                                                                             <  1:829538/238‑1 (MQ=255)                                                                 aGAT‑CGATCCCGCGACCTATCAGGCAAATTATGACAGCGCGAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCTCCCGAGGG‑TACGAAAT‑ACATCAGCCAGAAGGAGTACGACCAGGCCATTCATGATGCCCGCACGGCCGATGCCGCCGGGGTTGCGCAAAAAGCCAAAGTCGAAAGGGCCCGCCACAATCtgtctaaaaca                                                                                                                                                                          >  2:703355/1‑229 (MQ=255)                                                                 aGAT‑CGATCCCGCGACCTATCAGGCAAATTATGACAGCGCGAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATAcc                                                                                                                                                                          >  1:681795/1‑239 (MQ=255)                                                                 aGAT‑CGATCCCGCGACCTATCAGGCAAATTATGACAGCGCGAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAACCCCCCCCCCAACCTTGCTTATAcc                                                                                                                                                                          >  2:444634/1‑239 (MQ=255)                                                                 aGAT‑CGATCCCGCGACCTATCAGGCAAATTATGACAGCGCGAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATCGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAACCCACAGTCGCAACCGCTCGCATCAACCTTGCTTATAcc                                                                                                                                                                          >  1:557624/1‑239 (MQ=255)                                                                  gat‑cgatcCCGCGACCTATCAGGCAAATTATGACAGCGCGAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCCTTGCTGATGCCCGTCAGGCCGATGCCGCCGTGATTGCCGCACAAGCCCCAGTCGAAAGCGCCCCCCTCAAACTTGCTTATAACa                                                                                                                                                                         >  2:547874/1‑239 (MQ=255)                                                                  gat‑cgatcCCGCGACCTATCAGGCAAATTATGACAGCGCGAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCa                                                                                                                                                                         >  1:333747/1‑239 (MQ=255)                                                                   at‑cgatcCCGCGACCTATCAGGCAAATTATGACAGCGCGAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCaa                                                                                                                                                                        >  1:794393/1‑239 (MQ=255)                                                                   at‑cgatcCCGCGACCTATCAGGCAAATTATGACAGCGCGAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAACCCACAGTCGAAAGCCCTCGCATCAATCTTGCTTTAACCaa                                                                                                                                                                        >  1:882642/1‑239 (MQ=255)                                                                      cgatcCCGCGACCTATCAGGCAAATTATGACAGCGCGAAAGGCGAACTGGCG‑AAAAGTGAAG‑ACGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCCATGCCGCCGGGATTGCCGCCAAACCCCAAATCGAAAGCGCCCTAATCCATCTTGCTAATACaaaaa                                                                                                                                                                      >  2:135022/1‑238 (MQ=255)                                                                           ccGCGTCCTATCAGGCAAATTATGACCGCGGGAAAGGCGAACGGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACt                                                                                                                                                                 <  1:304701/239‑1 (MQ=255)                                                                              cgACCTATCAGGCAAATTATGAAAGCGCGAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GATAAAACGTTACGTTCCGCTCGTGGG‑GACGAAAT‑ACATCAGCCAGCAGGGGTACGACCAGGCCATTGCTGGTGCCCGTACGGCAGATTCACCCGTGTTGGCCGCCAAAGCCCCAGTCGACAGCGCGCCCACCAACCTTTTGTATACCaaaaccaaccc                                                                                                                                                               >  2:399855/1‑230 (MQ=255)                                                                                             aTTATGCCAGCGCGAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGATACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCCCGTCAGGCCGATGCCGCCGTGGTGGCCGCAAAAGCCACAGTCGAAACCGCCCGCGTCAACTTTGCTTTTCCCCAAACCACCGCGCCAATTAGCGGAca                                                                                                                                                >  2:651190/1‑237 (MQ=255)                                                                                                  ggcAGCGGAAAAGGCGAACGGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCgg                                                                                                                                          <  1:867484/237‑1 (MQ=255)                                                                                                  aacgccctaaaaggtgaatgtcgg‑AAAAGTGACGTCCCCCGCCCCCTCCGC‑CATTTGAC‑CGTAAAACTTTACGTTCCCCTTGTGGG‑TACAAAAT‑ACACCACCCACCAGGATTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCgg                                                                                                                                          <  2:118373/216‑1 (MQ=255)                                                                                                       cgcgAAAGGCGAACTGGCG‑AACAGCGAAG‑CCACCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGCAAT‑ACATCAGCCAGCAGGAGAACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAACCCACAACGGCAAGCGCTCGCATCAATCTTGATTTTCCCCAAAGCAACGCCCCACTAAGCGGCCGGATCGGCaaac                                                                                                                                     >  2:68424/1‑238 (MQ=255)                                                                                                       cgcgAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGCTGCCGCCGTGATTGCCCCAAAAGCCCCAGTCGAAAGCGCCAGCATCAATCTTTCTTAAACCAAAGACAAGCCGCCAAtaaaaggaagaaaacgccaaa                                                                                                                                     >  2:330919/1‑219 (MQ=255)                                                                                                       cgcgAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGcc                                                                                                                                                                                                              <  1:654194/166‑1 (MQ=255)                                                                                                       cgcgAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGcc                                                                                                                                                                                                              >  2:654194/1‑166 (MQ=255)                                                                                                       cgcgAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATAAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCCAAt                                                                                                                                     >  1:597076/1‑239 (MQ=255)                                                                                                       cgcgAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGCCAGGCCGATGCCGCCGGGATTGCCGCAAAAGCCACAGTCGAACACGCCCCCATCAATCTGCCTTAACCCAGAGTCACTGCGCCAATTACCGAACGTACCGGCAAAt                                                                                                                                     >  2:82753/1‑239 (MQ=255)                                                                                                       cgcgAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCCCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGGCGAAAGCGCCCCCATCAATCTTGCTTAACCCAAAGCAACTTCGCCAAATAACGGAGGGAACGGCaaa                                                                                                                                      >  2:448226/1‑238 (MQ=255)                                                                                                       cgcgAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCCCGTCAGGCCGATCCCGCCGTGATTGCGGCAAAACCCCAAATCGAAAGGCCTCGATCCCATCTTACTTATACCACAACCACTCCCCCAAttccagaccgtacgccaaaa                                                                                                                                      >  2:5949/1‑220 (MQ=255)                                                                                                         cgAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGTAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGCCGAAACCGCCGGCATAAACCTTGCTTATACCAAAGTAACTCGGCCAATTAACGGCAGAACCGGCAAAACg                                                                                                                                   >  2:692789/1‑239 (MQ=255)                                                                                                         cgAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCCCGCATCACACTTGCTTATACCAAAGTACCCGCGCCAATACACGGACGGATCGCCaaacac                                                                                                                                   >  2:589776/1‑236 (MQ=255)                                                                                                                 gAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCACCAATCTTGCTTATACCAAAGt                                                                                                                                                                     >  1:572457/1‑197 (MQ=255)                                                                                                                    cgggCG‑AAAAGGGAAG‑CCGGCGCCGCCACCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCCCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGa                                                                                                                        <  1:648187/237‑1 (MQ=255)                                                                                                                       ggcgcaaagggaaagcccgggacgccATCG‑GCACTTGACAGGTAAAAGCGTACGTTCCGCGGGTGGG‑TAAGAAATCGCCTC‑GCCAGAGGGAGTACGACCCGGCCAGTGTGGATGATCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGa                                                                                                                        <  2:333747/216‑1 (MQ=255)                                                                                                                             aggggAAG‑CCCCCGCCACCGTGGCGCCTTTGAC‑GGAAAACCGTTCCTTTCCGGTCGTGGG‑TACGAAAT‑ACGTCCACCAGACGGAGTACGGCGCGGCCATTGCTGATGCCCGTAAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGct                                                                                                                 <  2:37535/234‑1 (MQ=255)                                                                                                                                  acg‑ccccccccgccCTCGCGCATTTGAC‑GGTAAACCGTTACGTTCCGCTCGGGGG‑TACGAAAT‑CCATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGt                                                                                                            <  2:794393/236‑1 (MQ=255)                                                                                                                                  aaG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGGATCGGCAAATCTACTGTGACCGAAGGCGCCCTTTTc                                                                                                           >  1:116921/1‑239 (MQ=255)                                                                                                                                                cATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGACCGTCAGGCCGATGCCGCCGAGATTTCCGCAAAAGCCACAGTCGTAAGCGCTCGCATCCATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGAATCGGCAAATCACCTGTGACCGAAGACGCCCCTGGCACAAAAGGGCaaa                                                                                              >  2:268012/1‑239 (MQ=255)                                                                                                                                                     gtcttttGCC‑TGTAAAACCTTACGTCCCG‑TCGTGGGCGTCGAAAT‑CAATCATCCAGCAGGGTTACGACCAGGCCATTGCTGTTTCTGCGCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTAGCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGA‑Ct                                                                                        <  2:918456/235‑1 (MQ=255)                                                                                                                                                            gAC‑GGTAAAACGTTACGTTCCGCTCGGGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGctc                                                                                                                <  1:499258/209‑1 (MQ=255)                                                                                                                                                                            cgttCCGGTCGGGGG‑TACGAAAT‑TCCTCAGCCAGGAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGA‑CTGAACTGGCGACTGTCCagcagc                                                                  <  1:275251/239‑1 (MQ=255)                                                                                                                                                                             gttCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGA‑CTGAACTGGCGACTGTCCAGCAGCt                                                                 <  1:730515/239‑1 (MQ=255)                                                                                                                                                                             gttCCGCCCGTGGG‑TACGAAAC‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGA‑CTGAACTGGCGACTGTCCAGCAGCt                                                                 <  1:525615/239‑1 (MQ=255)                                                                                                                                                                             gttCCGCCAGTGGG‑TACGAAAG‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGA‑CTGAACTGGCGACTGTCCAGCAGCt                                                                 <  1:429002/239‑1 (MQ=255)                                                                                                                                                                                   cTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCCCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGAGGACGTATCGACAAATCGACTGTACACGAAGACCACCTTGTTACGAACGGACACACGA‑CGGACATGGCAAcagacccacaacagacacc                                                           >  2:332092/1‑221 (MQ=255)                                                                                                                                                                                      gTGGG‑TACGAAAT‑GCCTCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGA‑CTGAACTGGCGACTGTCCAGCAGCTCGATCCTAt                                                        <  1:444634/239‑1 (MQ=255)                                                                                                                                                                                      gTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGGGACCCAAGGCGCCCTTGTCACTAATGGGCAAACGA‑CTGAACTGGCGACCGCCCACCCGCTCGACCCTa                                                         >  2:665959/1‑238 (MQ=255)                                                                                                                                                                                             aCGAAAT‑CCATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGA‑CTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACg                                                   <  1:49618/238‑1 (MQ=255)                                                                                                                                                                                                   ttccATCACCCAGCGGGATTACGACCGGGCCTTTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGA‑CTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGAtg                                             <  2:882642/236‑1 (MQ=255)                                                                                                                                                                                                   ctcactCAGCCCGCAGGCGTACGACCAGCCCAGTGCTAATGCCCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAAGGGGCAAACGA‑CTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGAtg                                             <  1:486892/234‑1 (MQ=255)                                                                                                                                                                                                   attcagcagccagcAGGGGTCCGACCAGGCCATTGGTGAAGCTGGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGA‑CTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGAtg                                             <  1:5949/233‑1 (MQ=255)                                                                                                                                                                                                      cATCAGCCAGCAGGAGTACGACCAGGCCATTGCGGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGA‑CTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGa                                          <  1:486341/239‑1 (MQ=255)                                                                                                                                                                                                         cagccagcAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTtat                                                                                                                                                                             >  1:730406/1‑106 (MQ=255)                                                                                                                                                                                                         cagccagcAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTtat                                                                                                                                                                             <  2:730406/106‑1 (MQ=255)                                                                                                                                                                                                                cagGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGA‑CTGAACTGGCGACTGTCcagca                                                                    >  1:304490/1‑203 (MQ=255)                                                                                                                                                                                                                cagGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGA‑CTGAACTGGCGACTGTCcag                                                                      <  1:695644/201‑1 (MQ=255)                                                                                                                                                                                                                cagGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGA‑CTGAACTGGCGACTGTCcag                                                                      >  2:695644/1‑201 (MQ=255)                                                                                                                                                                                                                cagGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGA‑CTGAACTGGCGACTGTCCAGCAGCCCGATCCTATCTACGTTGATGGGA‑CCCAATCAAg                               >  1:23996/1‑239 (MQ=255)                                                                                                                                                                                                                cagGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGA‑CTGAACCGGCGACAGTCCAGCAGCCCGATCCTATCTACGTTGATGTGA‑CCCAAACCAg                               >  2:676064/1‑239 (MQ=255)                                                                                                                                                                                                                  ggcgtgccaCCCGGCCATTGCTAATGCCCGCGCGGCAGTGTCCGCCCTGATTGCCCCAAAAGCCACAGTCAAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGA‑CTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGA‑CCCAATCCAGCa                             <  2:116921/231‑1 (MQ=255)                                                                                                                                                                                                                   tttaggtgcggggcctttgtttgtGCTCGTCCGGCCGTGTCCGCCGTTATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGA‑CTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGA‑CCCAATCCAGCaa                            <  2:813468/216‑1 (MQ=255)                                                                                                                                                                                                                    aGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGA‑CTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGA‑CCCAATCCAGCa                             <  1:676064/237‑1 (MQ=255)                                                                                                                                                                                                                     gTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGCCTGTGACCGAAGGCGCCCTTGTCACTAATGGGCAAACGA‑CTGAACTGGCGACTGTCCACCAGCCCCATCCTATCTCCCTTCATGTGA‑CCCAACCCCGCAACg                          >  2:771001/1‑239 (MQ=255)                                                                                                                                                                                                                     gTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCCCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCCCTTGTCACTAATGGGCAAACGA‑CTGAACTGGCGACGTTCCAGCAGCCCGATCCTATCAACGTTTACTGGA‑CCCAAACcaacaaag                          >  2:254903/1‑237 (MQ=255)                                                                                                                                                                                                                      tACGACCAGGCCATTGCTGATGCTCGTCAAGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGCCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCCACTGAGACCGAAGGCCCTCTTGTCACTAATGGGCAAACGC‑ATGAACCGGCGACTGTCCGGCAGCTCGACCCTTTTCACTTTGATGGGA‑CCCAATCCAGCacaaa                         >  2:728790/1‑235 (MQ=255)                                                                                                                                                                                                                        cGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGA‑CTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGA‑CCCAATCCAGCAACGAtt                       <  1:515474/239‑1 (MQ=255)                                                                                                                                                                                                                            cAGGCCATTTCTAAGGCCCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGA‑CTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGA‑CCCAATCCAGCAACGATTTTAt                   <  1:254903/239‑1 (MQ=255)                                                                                                                                                                                                                            cAGGCCATTGCTGAGGCTCGTACGGCTGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCAGCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGA‑CTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGA‑CCCAATCCAGCAACGATTTTAt                   <  1:99033/239‑1 (MQ=255)                                                                                                                                                                                                                              ggCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGC‑CTGAACTGGCGACTGTCCAGCAGCCCGATCCTATCTACGTTGATGTGA‑CCCAATCCAGCAACGATTTTAtaa                 >  1:419263/1‑237 (MQ=255)                                                                                                                                                                                                                              ggCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGA‑CTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGA‑CCCAATCCGACACCGATTTTAtaa                 >  1:656128/1‑237 (MQ=255)                                                                                                                                                                                                                              ggCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGA‑CTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGA‑CCCAATCCAGCAACGATTTTAtta                 >  1:548677/1‑237 (MQ=255)                                                                                                                                                                                                                                    tgctgctgcTCGACAGGCCGATGCCGCAGTGATTGCAGCAAAAGCCACAGTCGAAAGCGCTCGAATCAATATTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCACATCGACTGTGACCGAAGGCGCCCTTGTCACTAAGGGGAAAAGGA‑CCGAACTGGCGACTGTCAACAAGCCCGACCCGACCCACATGGATGTGA‑CCCAATCCACCAAAGACTTAATAAggataa           >  2:281148/1‑235 (MQ=255)                                                                                                                                                                                                                                    tGCTGATGCTCGTCAGGACGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCCCGCATCAATCTTGCTTTTACCAAAATCACTGCGCCAATTCGCGGACCAAACGGCAAATCAACTGTAACCGACGCCCCTCTTG‑CACCAAATGGTCCACGACCTAAACTAGCGACTGCCCCGCCGCTCCATCCTTTACACGGTGATTGTA‑CCCAACCAAGGAAACATTTTaataggtaga           >  2:3158/1‑230 (MQ=255)                                                                                                                                                                                                                                     gccgATGCTCGACAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGA‑CTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGA‑CCCAATCCAGCAACGATTTTATGAGGCTGaa          <  1:332092/236‑1 (MQ=255)                                                                                                                                                                                                                                       tGATGCTCGTCAGGGCGATTCCGCCGTGATTGCCGCAAACGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGA‑CTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGA‑CCCAATCCAGCAACGATTTTATGAGGCTGAAGc        <  1:209368/239‑1 (MQ=255)                                                                                                                                                                                                                                         aTGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTCTACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCGCTTGTCACTAATGGGCAAACGA‑CTGAACTGGCGACAGTCCAGAAGCTCGTTCCTATTTACGTTG‑TTTGACCCCAAACCACCAAAGAtttattggggtgtgaacaa      >  2:297874/1‑223 (MQ=255)                                                                                                                                                                                                                                         aTGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGA‑CTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGA‑CCCAATCCAGCAACGATTTTATg                  >  1:813468/1‑227 (MQ=255)                                                                                                                                                                                                                                         aTGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGA‑CTGAACTGGCGACTGTCCAGCAGCCCGATCCTATTCACGTTGATGTGA‑CCCAAACCAGCAACAATTTTATGAGGCTGaacaaa      >  2:458401/1‑235 (MQ=255)                                                                                                                                                                                                                                         aTGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCAAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACAC‑CGGAACTGGCGACTGTCCACCAGCTCGATCCTATCTACGTTGAGGTAC‑CCCAATCCACCACAGATTTTTTGAGGCGGAAgaa       >  2:660571/1‑236 (MQ=255)                                                                                                                                                                                                                                          tGCCCGGCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGA‑CTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGA‑CCCAATCCAGCAACGATTTTATGAGGCTGAAGCAAt     <  1:665959/239‑1 (MQ=255)                                                                                                                                                                                                                                             tCGTCAGGCCTATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGA‑CTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGA‑CCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCg  <  1:589776/239‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                 GGATCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTA‑CCAGAT‑CGATCCCGCGACCTATCAGGCAAATTATGACAGCGCGAAAGGCGAACTGGCG‑AAAAGTGAAG‑CCGCCGCCGCCATCGCGCATTTGAC‑GGTAAAACGTTACGTTCCGCTCGTGGG‑TACGAAAT‑ACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGA‑CTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGA‑CCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCG  >  NC_000913/3414079‑3414546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑