Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,416,440	C→T	13.7%	F470F (TTC→TTT)	acrF →	multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,416,440	0	C	T	13.7%	193.2 / 4.8	73	F470F (TTC→TTT)	acrF	multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base C (13/49);  new base T (8/3);  total (21/52)
Fisher's exact test for biased strand distribution p-value = 1.38e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACACTAACGATGTTCGGGATGGTGCTTGCCATCGGGCTGCTCGTCGATGATGCGATAGTGGTGGTGGAGAACGTCGAGCGCGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCAGGGCGCACTGGTGGGTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCAT‑AGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACAGGACGATATTTACTGATCTATGCGCTGATTGTTGC  >  NC_000913/3416204‑3416676                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                  actctaaggtgtttgggggTGGTGGTTGCCATCGGGCTGCTCGTAGAGGATGCGATGGTGGGGGTGGAAAACGGGGGGGGGGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCAGGGCGCACTGGTGGGTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTtctc                                                                                                                                                                                                                                                 <  2:380641/224‑1 (MQ=255)  cacTAACGATGTTCGGGATGGTGCTTGCCATCGGGCTGCTCGTCGATGATGCGATAGTGGTGGTGGAGAACGTCGAGCGCGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATTTCGCAACTTCAGGGCGCACTGGCGGGTATC‑GCGCTGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGGTTCATC‑CGGGGCATTTTATCCCCGTTtcta                                                                                                                                                                                                                                                 >  2:213043/1‑238 (MQ=255)  cacTAACGATGTTCGGGATGGTGCTTGCCATCGGGCTGCTCGTCGATGATGCGATAGTGGTGGTGGAGAACGTCGAGCGCGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCAGGGCGAACTGGTGGGAATC‑GCGAGGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCCGTTttc‑t                                                                                                                                                                                                                                               >  1:694178/1‑239 (MQ=255)          aTGTTCGGGATGGTGCTTGCCATCGGGCTGCTCGTCGATGATGCGATAGTGGTGGTGGAGAACGTCGAGCGCGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCAGGGCGCACTGGTGGGTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTTTCA‑TGGGGGAATTTTTTCCCAGTtctttttta                                                                                                                                                                                                                                            >  1:833790/1‑229 (MQ=255)             ttCGGGATGGTGCTTGCCATCGGGCTGCTCGTCGATGATGCGATAGTGGTGGTGGAGAACGTCGAGCGCGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCAGGGCGCACTGGTGGGTATC‑GCGATGGTGCTGTCAGCGGAATTTATTCCGATGGAATTTTTCGGC‑GGGTTGACTTGGGGCATTTTTTC‑CCCGTTTTCTTATCACCACCg                                                                                                                                                                                                                                     >  2:450017/1‑239 (MQ=255)                     ggTGCTTGCCATCGGGCTGCTCGTCGATGATGCGATAGTGGTGGTGGAGAACGTCGAGCGCGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCAGGGCGCACTGGTGGGTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTTTC‑TATCACCCTCG‑TTTC‑TGCaa                                                                                                                                                                                                                          >  1:82377/1‑239 (MQ=255)                       tGCTTGCCATCGGGCTGCTCGTCGATGATGCGATAGTGGTGGTGGAGAACGTCGAGCGCGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGGGACGGAAAAATCGATGGCGCAAATTCAGGGCGCACTGGAGGGTATC‑GCGATGGTGCTGTCAGGGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTTC‑TGGGGGCATTTATTGGCCGTTCTC‑TATAAACATCA‑CTTC‑GGCaaag                                                                                                                                                                                                                        >  2:478714/1‑237 (MQ=255)                         cTTGCCATCGGGCTGCTCGTCGATGATGCGATAGTGGTGGTGGAGAACGTCGAGCGCGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCAGGGCGCACTGGTGGGTATC‑GCGATGGTGCTGTCAGAGGTATTTATTCCGATGGAATTCTTCGGC‑GGTTCGAC‑TTGGGACATTTATTGCCCGTTTTA‑TTTTACCATCG‑GTTC‑GGCAATGgc                                                                                                                                                                                                                      >  1:494656/1‑239 (MQ=255)                              cATCGGGCTGCTCGTCGATGATGCGATAGTGGGGGTGGAGAACGTCGAGGGCGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCAGGGCGCACTGGTGGGTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCttt                                                                                                                                                                                                                 <  1:675071/239‑1 (MQ=255)                                 cGGGCTGCTCGTCGATGATGCGATAGTGGTGGTGGAGAACGTCGAGCGCGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCAGGGCGCACTGGTGGGTATC‑GCGATGGTGCTGTAAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TTGGGGGATTTGATGGCAGTTTTC‑TCTCACCCTCGCTTTC‑GGCatgggcctttttg                                                                                                                                                                                                               >  2:141283/1‑227 (MQ=255)                                        gccgtcaggggtgCGATAGGGGGGGGGGAAAACGTCGAGCGCGGGATGAGGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCAGGGCGCACTGGTGGGTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCt‑gg                                                                                                                                                                                                       <  1:150402/227‑1 (MQ=255)                                              gatgatGCGATAGGGGTGGTGGAGAACGGCGAGGCGGTGAGGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGGCGCAAATTCAGGGCGCACTGGTGGGTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCAt                                                                                                                                                                                                 <  1:280652/238‑1 (MQ=255)                                                     ggtggggggtgggggGAAACGAGGAGCGGGTAATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCAGGGCGCACTGGTGGGTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCt                                                                                                                                                                                          <  1:607851/231‑1 (MQ=255)                                                     ggaaggggggggtggAGAACGTCGAGCGCGGGAAGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGGCGCAAATTCAGGGCGCACTGGTGGGTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCt                                                                                                                                                                                          <  1:377685/228‑1 (MQ=255)                                                     cGATAGTGGTGGTGGTGAACGTCGGCCGTGTTAGGGAGGAGGATAAGCTTCCGGCAAAAGGAGCCACGGAAAAATCGATGTCGCAAATTCAGGGCGCACTGGTGGGTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCt                                                                                                                                                                                          <  1:796352/238‑1 (MQ=255)                                                        tAGTGGGGGTGGAAAACGGCGAGCTCGGTATTATGGAGGAGAAGCTCCCGCCAAAAGAAGCGACGGAAAAAGCGATGTCGCAAATTCAGGGCGCACTGGTGGGTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTAcc                                                                                                                                                                                      <  1:918573/239‑1 (MQ=255)                                                         aGTGGTGGGGGAAAACGTCGGGGGGGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCAGGGCGCACTGGTGGGTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTAccc                                                                                                                                                                                     <  1:547962/239‑1 (MQ=255)                                                           gggtgggggGGAACGTCGAGGCCGGGAGGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCAGGGCGCACTGGTGGGTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTAccc                                                                                                                                                                                     <  1:1623/236‑1 (MQ=255)                                                               ggtggAGAACGTCGAGCGCGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCAGGGCGCACTGGTGGGTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCg                                                                                                                                                                                <  1:213043/238‑1 (MQ=255)                                                               ggtggAGAACGGCGGGCGCGTGAGGATGGAGGAGAGGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCAGGGCGACCGGGTGGGTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCg                                                                                                                                                                                <  1:850588/238‑1 (MQ=255)                                                                 tgaaaaccggcgaggcggTGATGGTGGAGGATAAGCTCCCGCCAAAAGAAGGTACGGAAAATTCGATGTCGCAAATTCAGGTCGCCGTGGTGGGTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGtt                                                                                                                                                                              <  2:418593/221‑1 (MQ=255)                                                                      aaCGTGGAGGGGGTGATGATTGAGGGAAAGCTCCCGCCAAAAGAAGGGACGGAAAAATCGATGTCGCAAATTCAGGGCGCACTGGTGGGTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTAtgtg                                                                                                                                                                         <  1:382781/238‑1 (MQ=255)                                                                        cggcgggggggtggtgATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATTTCGCAAATTCAGGGCGCACTGGTGGGTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCaa                                                                                                                                                                      <  1:697027/229‑1 (MQ=255)                                                                          gaggggcggggaggagggaggaTAGGCCCCCGCCAAAAGAAGGGACGGAAAAAGCGATGTCGGAAATTCAGGGCGCACTGGTGGGTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACg                                                                                                                                                                    <  2:759240/223‑1 (MQ=255)                                                                           tggggggttgttgatgGAGGATAAGCTTCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCAGGGCGCACTGGTGGGTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACg                                                                                                                                                                    <  2:56053/227‑1 (MQ=255)                                                                           cggccggttgatattgGGGGAAAAGCGCCCGCCAAAAGAAGCGACGGAAAACTCGATGTCGAAAATTCAGGGCGCACTGGGGGGGTTC‑GGTATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACg                                                                                                                                                                    <  2:874506/224‑1 (MQ=255)                                                                           cGAGCGTGGGAAGATGGAGGATAAGCTCCCGCCAAAAGGAGCGACGGAAAAATCGATGTCGCAAATTCAGGGCGCACTGGTGGGTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACg                                                                                                                                                                    <  1:635034/238‑1 (MQ=255)                                                                           cGAGCGCGTGATGATGGAGGATAAGCCCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCAGGGCGCACTGGTGGGTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACg                                                                                                                                                                    <  1:856862/238‑1 (MQ=255)                                                                                        atgGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCAGGGCGCACTGGTGGGTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGt                                                                                                                                                      <  1:246892/239‑1 (MQ=255)                                                                                         tggggaaTAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCAGGGCGCACTGGTGGTTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGt                                                                                                                                                      <  1:46969/232‑1 (MQ=255)                                                                                           gtggaTAACCCCCCGCCAAAAGGACGCACGGAAAAATGCATGGCGGAAATTCAGGGCGCACTGTTGGGTTTC‑GCGATGGTGCTGTCAGAGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCAACG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGtct                                                                                                                                                    <  2:859228/236‑1 (MQ=255)                                                                                                  aGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCAGTGCGCACTGGTGGGTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTCT‑TTGGGACATTTATCGCCCGTTTTC‑TATCTACCTCT‑TTTT‑GGCCATAGCGCTTTCTCTTCT‑GGTGGCGTTTATTCTTTCCCCTCCGTTTTTTGCAAACATGCTTTAACccctccctcttgat                                                                                                                                            >  2:263927/1‑233 (MQ=255)                                                                                                  aGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCAGGGCGCACTGGTGGGTATC‑GAGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTTCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAg                                                                                                                                            <  1:529797/239‑1 (MQ=255)                                                                                                        cGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCAGGGCGCACTGGTGGGTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGGAGTTTTTTTCCAGTTTCT‑TATCACCCTCG‑GTTCTGGAAAGGGGGTTTTTTGTT‑T‑TGTGGCGTTTTGTTTTACCCCCGCGTTCTTTGTAACCCTTCTTAAAACCGTGTCTGGTTGGCATCAc                                                                                                                                      >  2:25239/1‑239 (MQ=255)                                                                                                              aagaagCGACGGAAAAATCGATGTCGCACATTCAGGGCGCACTGGTGGGTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTTTAC‑TGGGGCAATTTATCTCCGGTTTTC‑TTTCACCATCG‑TTTC‑GGGAATGGAGCTTTTTGTTCTGGGTGGCA‑TGTGTCTTAATCCTGTGGTATGTGCAACGCTTCTTTAAACCGCCTctggttgcgatagcggaaaa                                                                                                                                >  2:654454/1‑222 (MQ=255)                                                                                                                  agCGACGGAAAAATCGATGTCGCAAATTCAGGGCGCACTGGTGGGTATT‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAgg                                                                                                                            <  1:276600/239‑1 (MQ=255)                                                                                                                                tggtggcGCAAATTCGGGGCGCACGGGGGGGTTTC‑GGGATGGGTGTGGCAGGGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTTTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCggt                                                                                                              <  2:221407/233‑1 (MQ=255)                                                                                                                                tcaTGTCCGAAATTCAGTGCTCCCTGGTGGGTTTC‑GGGTGTGTTGTGCAAGCGGTATTTATTCCGATGGCATTCTTCGGC‑TGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTTGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCggt                                                                                                              <  1:428819/237‑1 (MQ=255)                                                                                                                                cgaggtccgaatttCAGGGCGCACTGGTGGGTATC‑GCGATGGTGCAGTCAGCGGTATTTTTTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCggt                                                                                                              <  1:597366/227‑1 (MQ=255)                                                                                                                                cGATGTCGCAAATTCAGGGCGCACTGGTGGGTATC‑GCGATGGTTCTGGCAGCGGTATTTATTCCGATTGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCCCCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCggt                                                                                                              <  1:360475/239‑1 (MQ=255)                                                                                                                                cGATGTCGCAAATTCAGGGCGCACTGGTGGGTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCggt                                                                                                              <  1:25239/239‑1 (MQ=255)                                                                                                                                cGATGTCGCAAATTCAGGGCGCACTGGTGGGTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCggt                                                                                                              <  1:56303/239‑1 (MQ=255)                                                                                                                                cGATGTCGAAAATTCAGGGGGCGCTGGGTGGTATG‑GGGAGGGTGCTGTGAGGGGTTTTTATTCCGAGGGCATTCTTCGGC‑TGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑GTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCggt                                                                                                              <  2:132263/239‑1 (MQ=255)                                                                                                                                    gTCGCAAATTCAGGGCGCACTGGTGGGTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATTACCATCG‑TTTC‑GGCAATGGCGCTTTCTTTTCT‑TGTGGCATTGATTCTTAACCCTTCGTTATGTGCAACGCTGCTTAAAACCGTCTCTTCTGCGCATCACGAAAATAAAGGCGGTTTCTTCggtttgg                                                                                                          >  1:580101/1‑236 (MQ=255)                                                                                                                                           tttttggggcttttgttggttgtC‑GCGATGGTGTTGGCTGCGGTATTTATTCCGTTGTCATTTGTCGGC‑GGTTCTGC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCT‑GTTC‑GGCAATGGCGCTTTTTGTTCT‑GGTGGCATTGATTCTTTCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGCTTTCTTCTCTTGGTTTAATac                                                                                                   <  2:494656/217‑1 (MQ=255)                                                                                                                                           aTTCAGGGCCGACTGGTGGGTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGTCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATac                                                                                                   <  1:478714/239‑1 (MQ=255)                                                                                                                                                  gcccACTGGTGGGTATC‑GCGATGGTGTTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTTC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTTATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCtt                                                                                             <  1:64210/235‑1 (MQ=255)                                                                                                                                                       cTGGTGGGTTTCGGGGTTGGTCTTGTC‑GCGGTATTTTTTCCGATTGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCCCCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCa                                                                                       <  2:717649/239‑1 (MQ=255)                                                                                                                                                       cTGGGGGGTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTTTTCGGC‑GGTTCTAC‑TGGGGCAATTTAGCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCa                                                                                       <  1:141283/239‑1 (MQ=255)                                                                                                                                                        tggtggGTATC‑GAGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCCTCGGC‑GGTTCTAC‑TGGGGCCATTTATCGCCGGTTCTC‑TATCCCCATCG‑GTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCAt                                                                                      <  1:518060/239‑1 (MQ=255)                                                                                                                                                         ggggggTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCCCCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCAT‑a                                                                                    <  1:469250/236‑1 (MQ=255)                                                                                                                                                          gtggGTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCt                                                                                                                                                                                          <  1:298944/137‑1 (MQ=255)                                                                                                                                                               gtataagagcacggCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCAT‑AGCGtt                                                                               <  1:415854/226‑1 (MQ=255)                                                                                                                                                                  c‑gcgATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAT‑CGGGGCTATTTATCGCCCGTTCTC‑TATCCCCCTCT‑TTTC‑GGCAATGGCGCTTTTTGTTTT‑GGTGGCATTGGTTCTTTCCCCCGCGGTTTTTGCACACCTCCTTTAACCCGTCCCTTCTCACTTTTACGCAAACAAGGGCGGGTTCTTTCGTTGGGTTTATATCACACTCGCTCCT‑AGCGGTAAca                                                                           >  2:175946/1‑238 (MQ=255)                                                                                                                                                                  c‑gcgATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTTTTGCCCGTTCTC‑TATCACCCCCG‑TTTC‑TGGAATGGCGCTTTTTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTTTTTGCAACGCTGCTTTAACCCGTCTCTGCTGAGCCTCACGAAAAAAAGGGGGGGTTTTTCGGTTGGGTTTTTACCCCCCTCGATCAT‑AGCGTTaaac                                                                           >  2:597495/1‑237 (MQ=255)                                                                                                                                                                  c‑gcgATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATGGCCCGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGGAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGGTTCTTCGGGTGGTTTAATACCACCCTCGTTCAA‑AAAGTTaaac                                                                           >  1:909006/1‑237 (MQ=255)                                                                                                                                                                  c‑gcgATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCAT‑AGCGTTAAcc                                                                           <  1:837108/239‑1 (MQ=255)                                                                                                                                                                   cccggtGGTGCTGTCAGCCGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGTGCAATTTATCGCCATTTCTC‑TATCCCCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCGTCGGTTGGTTTAATACCACCTTCGATCAT‑AGCGTTAAcc                                                                           <  1:104318/234‑1 (MQ=255)                                                                                                                                                                     cgATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTTTAC‑TTGGGCAATTTTTCTCCAGTTTTC‑TTTCCCCCTCC‑GTTC‑GGCAATGGCGCTTTCTTTTCT‑TGGGGCATTTATTTTTTCCCCTGCg                                                                                                                                                                                >  2:298944/1‑137 (MQ=255)                                                                                                                                                                     cgATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTTT‑TGGGGGAATTTATCTCCCGGTCTT‑TTTCACCACCG‑ATTC‑GGGACTGGCGCTTTCTGTTCT‑TGTGGCATTGATTCTTCACCCTGCGTTTTGTGGCACGCTGCTTCAACCCCTCTCTTCTTTGCACCCCGAAAATAAAGGCGGTTTCTTGGGGTGGTTTTATACCACCTTctcccctcacgttatccc                                                                           >  2:490635/1‑221 (MQ=255)                                                                                                                                                                      ggtggggcgttcagggggtttttttCCGGTGGCATTTCTCGGG‑TGTTTGAC‑TGGGGCAATTTTTAGCCAGGTTCA‑TTTCCACATCG‑GTTC‑GGCAATGGCGCTTTCTGGTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCAT‑AGCGTTAACCAc                                                                         <  2:160578/219‑1 (MQ=255)                                                                                                                                                                      ggtggggattgtaGCGGTATTTATTCCGATGTCATTCTTCGGC‑GGTTCTAC‑TGGGTCAATTTATCGCCAGTTCTC‑TATCGACATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑TGTGGCATTGATTCTTACCCCGGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCAT‑AGCGTTAACCAc                                                                         <  1:263927/226‑1 (MQ=255)                                                                                                                                                                                 cAGGGGTTTTTTTTCCGGTGTCATTCTTCGGC‑GGTTCTAC‑TGGTGCAATTTCTCGCCAGGTCTC‑TATCACCGTCT‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCAT‑AGCGTTAACCACTACACCAACAGc                                                             <  2:344433/239‑1 (MQ=255)                                                                                                                                                                                    cGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCCGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGAGGGTTCTTCCGTTTGGTTATTTACCCCTCCGTTCAA‑AGAGTTaaacccaaaaacaaaaaggga                                                          >  2:215642/1‑220 (MQ=255)                                                                                                                                                                                             ttCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAAGTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTTCCCCTGCGTTAtgtg                                                                                                                                                                         <  1:729525/120‑1 (MQ=255)                                                                                                                                                                                                 gATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTTTCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATTGCGCTTTTTGTTCT‑GGGGGCATTGATTCTTACCCCCGCGTTTTGTGCAACCCTGCTTAAACCCGTCTCTGCTGAGCATCCCGAAAATAAGGGCGGGTTCTTCGGTTGGTTTTTTACCACCTTCGCTCATAAGGGTTAACCCCAACACaaaaacctccgccaaaaacccg                                              >  2:792356/1‑220 (MQ=255)                                                                                                                                                                                                       aTTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCCCCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGGGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCAT‑AGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCca                                       <  1:792356/239‑1 (MQ=255)                                                                                                                                                                                                             cggc‑ggTTCTAC‑TGGGGCAATTTATCGCCAGCTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCGTTCTGTTCC‑GGTGGCACTGATTCTTAACCCTGCGTTATGTGCAACGCTGCTTAAACCCTTCCCTGCTGAGCATCACGAAAATAAGGGCGGGTTCTTCGGTTGGTTTTATACCCCCCTCTCTCAT‑AACGGTCACCACTACACCAACCGCCTCAGTCAAATCCCCCGACCCACAAGAc                                 >  2:376265/1‑239 (MQ=255)                                                                                                                                                                                                                  ggTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCAT‑AGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACAGGACGat                              <  1:597495/238‑1 (MQ=255)                                                                                                                                                                                                                      cTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGTTCAT‑AGCGTTAACCACTACACCAACAACGTCGGCAAAATCCTCGGATCCACAGGACGATAtttt                         >  1:190081/1‑238 (MQ=255)                                                                                                                                                                                                                         ttttttttaatggccatctggTTTTC‑TCTCCCCTTCT‑TTTC‑GGCAATGGGCCTTTTTGTTCT‑GGTGGCATTGCTTCTGACCCCTGCGTTATGTGCATCGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCAT‑AGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACAGGACGATATTTACt                       <  2:814854/219‑1 (MQ=255)                                                                                                                                                                                                                            ggggCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCAT‑AGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACAGGACGATATTTACTGAt                    <  1:215642/239‑1 (MQ=255)                                                                                                                                                                                                                               gCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCAT‑AGCGTTAACCACTACACCAACAGCGCCGGCAAAATCCTCGGATCcaaaagcaaaaatttacctacata                 >  2:436836/1‑217 (MQ=255)                                                                                                                                                                                                                                  aTTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCAT‑AGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGCTCCACAGGACGATTTTTCCTGACCTATg               >  1:916789/1‑238 (MQ=255)                                                                                                                                                                                                                                        cGCCAGTTTTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCAT‑AGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACAGGACGATATTTACTGATCTATGCGCTGAt        <  1:436836/239‑1 (MQ=255)                                                                                                                                                                                                                                        cGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCTT‑AGCGTTAACCACTACACCAACAGCGTCGGCAAAATCTTCGGATCCACAGGACGATTTTTACTGATCTATGCGCTGAt        >  1:237828/1‑239 (MQ=255)                                                                                                                                                                                                                                             gTTCCC‑TATCCCCATCG‑TTT‑‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCAT‑AGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACAGGACGATATTTACTGATCTATGCGCTGATTGTTGc  <  1:638890/239‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                  ACACTAACGATGTTCGGGATGGTGCTTGCCATCGGGCTGCTCGTCGATGATGCGATAGTGGTGGTGGAGAACGTCGAGCGCGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCAGGGCGCACTGGTGGGTATC‑GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGC‑GGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTC‑TATCACCATCG‑TTTC‑GGCAATGGCGCTTTCTGTTCT‑GGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCAT‑AGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACAGGACGATATTTACTGATCTATGCGCTGATTGTTGC  >  NC_000913/3416204‑3416676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑