| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,479,687 | G→A | 22.4% | L307F (CTC→TTC) | kefB ← | potassium:proton antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,479,687 | 0 | G | A | 22.4% | 75.8 / 2.9 | 38 | L307F (CTC→TTC) | kefB | potassium:proton antiporter |
| Reads supporting (aligned to +/- strand): ref base G (20/9); new base A (2/7); total (22/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.11e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTATCCACCAGCTTCATCAGCAACGGCGTGGTCATCATGGAAAGCGTCACCGTCACCAGCAACAACGCCATCTGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGAT‑GGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGA > NC_000913/3479472‑3479924 | tttccccaccgacctactccgCAACGGGGGGGTCCACAGGGAAAGCCTCCCCCTCCCCAGCCACAACCCCATCGGGGCCCCCTGGAAAAAGCGTGGGAAAGAAGCGGTAGAAAAGAGGAAAAAGGAAAACCCCCCCCCCTGACCCAACACGCCAGCAAACTGCATCCGCTCGGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACaa < 2:198337/219‑1 (MQ=255) tATCCACCAGCTTCATCAGCAACGGCGTGGTCATCGTGGAAAGCGTCACCGTCACCAGCAACAACGCCATCTGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCCCTGAGCTCCCCACGCCATACAATCGCGCCCGCAGAACAACCACGAAAATTTTAACC‑ccaccaaccacaac > 2:160213/1‑230 (MQ=255) tATCCACCAGCTTCATCAGCAACGGCGTGGTCATCATGGAAAGCGTCACCGTCACCAGCAACAACGCCATCTGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCCCTGACCTACCCACGCCATCCAAACCCCCCAGCAGAACAACCACGAAAATTTTCACCGccaccacaccaaca > 2:191707/1‑231 (MQ=255) cccacaGCTTCAGCGGCAACGGCGTGGACATAAGGGAAAGCGTCCCCGGCACCAGCAACAACGCCATCTGGTCGCCCTGGAATAGGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACg < 1:439875/235‑1 (MQ=255) cTTCATCAGCAACGGCGTGGTCATCATGGAAAGCGTCACCGTCACCAGCAACAACGCCATCTGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGAACGAGAATTTTCACCGCCACCAGCACAACCAGCCTTATc > 1:866034/1‑238 (MQ=255) gTCATCATGGAAAGCGTCACCGTCACCAGCAACAACGCCATCTGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCAAGCCATACAATCCCGCCAGCAGATACAGCACGAGAATTTTCACCCCCACCAGCCCAACCACGCTTATCAcctggctcttatacacat > 1:21193/1‑221 (MQ=255) gCGTCACCGTCACCAGCAACAACGCCATCTGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCCCCAGCACAACCACCCTTATCACTACCCACAACAGAT‑ggggtaaaaaaaacccc > 2:110774/1‑225 (MQ=255) cgcAGAAACAACGCCATCGGGGCCCCATGAAAAAAGCGTTGGAAAGAAGCGGTAGAAAAGAGGACAAAGCCAAACTCACCCCCCGGACTAAACCCGCAAGCAAACTGAATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGAT‑GGGTATAAAGCACCCCGAGGTTGAGTGa < 1:296197/237‑1 (MQ=255) aacaacGCCATCTGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGCGAATTTTCACCGCCACCAGCACAACCAAGCTTATCACTACCCACAACAGAT‑GGGTATAAAGCACCCCGCGGTTgctcgacggcct > 1:528145/1‑228 (MQ=255) gaaaagggaaaaaacgcaaaaaaGAGAGAAAAGGCAAACCCACCCCCCTGACCGAACCGCCCAGAAAAGTGGCTCCCGTCTAAGCTAAGCACCCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGAT‑GGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAgcagca < 1:729215/219‑1 (MQ=255) agaagCGGTAGAAAAGAGGACAAAGGCAAACTCACCCCCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGAT‑GGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTg < 1:191707/238‑1 (MQ=255) cGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTTAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCACCACAACCCCGCTTATCACCACCCACAACATAT‑GGGCTTAAAGAACAACGAGGTCGGGCGAACTTCCCACAGAGATAAAGAAAAAAACCAGAAGCAATCCTTTGACgggg > 2:639757/1‑238 (MQ=255) agagGAAAAAGGCAAACGCCCCCCCCTGACCCAACACGCCAGCAACCTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGAT‑GGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGcc < 1:160213/239‑1 (MQ=255) aGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCAAGCTTATCACTACCCACAACAAAT‑GTGACTAACGAACCCCGAGGCTGATTGCCCTACCGACAGATATAACAAACAAACAGATCAGCACGCCTTTGaaactgcatcttttacacaactaa > 2:729215/1‑217 (MQ=255) aaCTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGAT‑GGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGCCAGAGATAAAGAACAAACCGACCAGCAAGCCTTTGAAGGGATAGAAAGCCGTTTCCAGtgaagg > 2:616271/1‑234 (MQ=255) tGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGAT‑GGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGGGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCATTTCATGGCGATATTCACt > 1:858734/1‑239 (MQ=255) tGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGAT‑GGGTATAAAGCACCCCGAGGTGGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGAAAGCCTTTAAAGGGATCGATAACCGTTTCCAGTTCATGCCGATATTCACt > 1:578211/1‑239 (MQ=255) gACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGAT‑GGGCATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGAGCGAGAGCCGTGTCCaatgcaggcgaagatacaatt > 2:774834/1‑219 (MQ=255) ccAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGAT‑GGGTATAAAGCACCCCGAGGTTGGGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCAAGTTCATGGCGAAATCAACTTTCCGCcagaac > 2:228151/1‑236 (MQ=255) ccAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGAT‑GGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGGTAACCGTTTCCCATTCATGGGAAAATTACCTTTCCGcaccaag > 2:745165/1‑233 (MQ=255) actgcaaccctctcatgtgtgcccacgcCATAAAACCGGCCCAGCAGATACAGCACGAAAATTTTCACCGCCACCAGCACAACCCCGCTTATCACTACCCAAAACAGAT‑GGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGcc < 2:622951/216‑1 (MQ=255) gCACCCGCTTTGAGCTCCGCACGCCATACAATCGCGCCAGCAGATACAACACGAAAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGc < 1:110774/239‑1 (MQ=255) ctctGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAAcc > 2:91329/1‑73 (MQ=255) ctctGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAAcc < 1:91329/73‑1 (MQ=255) cgcacgcCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGAT‑GGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAggg < 1:363184/164‑1 (MQ=255) cgcacgcCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGAT‑GGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAggg > 2:363184/1‑164 (MQ=255) cgcacgcCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGAT‑GGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAgcg > 1:539734/1‑238 (MQ=255) gcCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGAT‑GGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGACCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATcc > 1:681931/1‑238 (MQ=255) ggcCAGCAAATCAAGCACGGAAATTTTCACCGCCACCAGCACAACCACGCTTAACACTACCCACAACAGAT‑GGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAgcg < 1:745165/238‑1 (MQ=255) gccccccaataaccaccagcgaATTTTCACCGCCCCCAGCCCACCCACGCTTATCGCGACCCACAACAGAT‑GGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAgcg < 2:741693/219‑1 (MQ=255) cgcCAGCAGATACAGCACGAGAATTTTCCCCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGAT‑GGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAgcg < 1:774834/239‑1 (MQ=255) cgcCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGAT‑GGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTCCCGAGCGCCATCGAAAGCCCCCgc > 1:377205/1‑238 (MQ=255) cgcCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGAT‑GGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCCTCGACACCCCCacc > 2:649328/1‑236 (MQ=255) cgcCAGCAGATACAGCACGAAAATTTCCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGAT‑GGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAgcg < 1:616271/239‑1 (MQ=255) cgcAAGCAGATACAGCCCGAAAATTTTCCCCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGAT‑GGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAgcg < 1:228151/239‑1 (MQ=255) cgacgaaagATACAGCCAGAAGATTTTCACCGCCACCAGCACAACCACGCTTATCCCTACCCAAAACAGAT‑GGGTAAAAAGCACCCCGAGGTTGAATGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAgcg < 1:468054/232‑1 (MQ=255) cccacgcaaaaaccacACGAAAATTTTCACCGCCACCAGCACAACCCCGCTTATCACTACCCACAACAGAT‑GGGTAAAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAgcg < 2:866034/224‑1 (MQ=255) ccAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGAT‑GGGTATAAAGCACCCCGAGGTTGAGTGAAATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCCATAAATGTACCGAGCGCCATCGACAGCCCCAgcgc > 1:741693/1‑238 (MQ=255) cacgaATTTTCCCCCCCCCCAGAACAACCACGCTTATCACTACCCACAACAGAT‑GGGTATAAAGCTCCCCGAGGTTTAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCg < 2:445619/236‑1 (MQ=255) cGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGAT‑GGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGACGGCCATCGACATCCCCAGCCCCACCATAAACAATGCg > 1:385995/1‑239 (MQ=255) gaATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGAT‑GGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGGACCGAGCGCCATCGACAGCCCCCGCGCATCCATAAACAATGCGGa > 1:590935/1‑238 (MQ=255) | TTTATCCACCAGCTTCATCAGCAACGGCGTGGTCATCATGGAAAGCGTCACCGTCACCAGCAACAACGCCATCTGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGAT‑GGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGA > NC_000913/3479472‑3479924 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |