Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,536,121 G→C 19.7% R154R (CGC→CGG ompR ← response regulator in two‑component regulatory system with EnvZ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,536,1210GC19.7% 93.9 / 3.1 55R154R (CGC→CGGompRresponse regulator in two‑component regulatory system with EnvZ
Reads supporting (aligned to +/- strand):  ref base G (20/22);  new base C (9/2);  total (30/25)
Fisher's exact test for biased strand distribution p-value = 8.60e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.85e-01

AGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGCGGAACATTTCGCGCGTACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCA  >  NC_000913/3535889‑3536353
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                            
aGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGCTGGCTGACCAGTGCCTTCAGTACCGCAAACTCAACGCTGGCGAGCGGCATCGGCGCGTATTCCCCGAAc                                                                                                                                                                                                                                        >  1:731566/1‑239 (MQ=255)
     tAGCCCAGACCCCAGACGGTCTGTATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGCGAGCGGCATCGGCTCGCCTTCCCCGAACActt                                                                                                                                                                                                                                    >  1:528612/1‑238 (MQ=255)
     tAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCAGCTCGTCTTTGCGGGACATTTc                                                                                                                                                                                                                                   >  1:89253/1‑239 (MQ=255)
     tAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGGGCCTTCAGTACCGCAAACTCACCGCCGGGAAGCGGCATCGGCCCCGCTTCGCGGCACAATTc                                                                                                                                                                                                                                   >  2:688300/1‑239 (MQ=255)
        cccagcccccaggcGGGCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGCGGAACATTTcgc                                                                                                                                                                                                                                 <  1:30287/238‑1 (MQ=255)
            gaccccagacGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGGGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCCCGGTCTTCCGGAAAAttttcctgct                                                                                                                                                                                                                             >  1:446044/1‑231 (MQ=255)
             accccagacGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCGCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGATCGGCATCGGCTCGTCTTTGCCGAGCATTTcgcgcgga                                                                                                                                                                                                                          >  1:756489/1‑236 (MQ=255)
                  agacGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGCGCGGCATCGGCCCCGCGTCCCGGGACAATTCACGCGGCCCGAgg                                                                                                                                                                                                                    >  1:675065/1‑239 (MQ=255)
                  agacGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCCCGGAAAATTTCGCGCGTACCGAgg                                                                                                                                                                                                                    >  1:568467/1‑239 (MQ=255)
                  agacGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGCCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTTAGCGGCATCGGCTCGGCTTCGCCGGACATTTtgccccgtccgcgg                                                                                                                                                                                                                      >  1:25534/1‑228 (MQ=255)
                   ggcggttggAATGTAAAGCGGGTGCGCTGGATTTTCTTCCCCCATGGGGCGCAGACGCGAAATCTGCCAGTGGATTGGGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGCGGAACATTTCGCGCGTACCGAGGt                                                                                                                                                                                                                   <  2:232472/231‑1 (MQ=255)
                   ggccggtttaagttAACCCGGGGGCGCTGGGTTTTCCTCCACCATGCGGCGCAGACGGGAAATCTGCCCGGCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGCGGAACATTTCGCGCGTACCGAGGt                                                                                                                                                                                                                   <  2:609368/226‑1 (MQ=255)
                    tcggagatgggtataagaggcagcgcTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGATCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGCGGAACATTTCGCGCGTACCGAGGt                                                                                                                                                                                                                   <  1:462954/216‑1 (MQ=255)
                     cGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGCGGAACATTTCGCGCGTACCGAGGt                                                                                                                                                                                                                   <  1:514235/237‑1 (MQ=255)
                        tCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGGCTTCCCGGAACATTTCTCGCGGACCGCGGGTAAGt                                                                                                                                                                                                              >  1:237053/1‑239 (MQ=255)
                         cTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCCTCTTTCCGGAACATTTTCCCCGTACCGCGGTTAAGtt                                                                                                                                                                                                             >  1:542586/1‑239 (MQ=255)
                             atttAACGTGGGTGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCAGGGCGATGGAGCGTTCCATTGCGGAAGATTGCCGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGCGGAACATTTCGCGCGTACCGAGGTTAAGTTTGaa                                                                                                                                                                                                         <  2:260872/236‑1 (MQ=255)
                              tgcaacgcggtcgctctgctccgtcttcCACCATGCGGCGCATACCAGAAACCTGCACGTCGATGGAGGTGTCCATTGGGTAATATTCACGACCACGGGCAAGGTTGATCAGCTTATCGGGGGAGAGCGGCGCACGCGGATGGCTGACCAGTGCCTTCAGTCCCGCAAACTCCCCGCTGGTGAGCGGCATCGGCTCGTCTTCGCGGAACATTTCGCGCGTACCGAGGTTAAGTTTGAAc                                                                                                                                                                                                        <  2:532467/216‑1 (MQ=255)
                                  aCGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGCGGAACAATTCGCCCGTACCGCGGTTACGTTTTGACttc                                                                                                                                                                                                     >  1:225022/1‑237 (MQ=255)
                                       gATGCGCTGGATCTTCTTCCACCAGGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGCGGAACATTTCGCGCGTACCGAGGTTAAGTTTGAACTTACCGaa                                                                                                                                                                                               <  1:245457/238‑1 (MQ=255)
                                                  tcttctTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGGGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTTGCGGAAAATTTCCCGCGGACCGAGGTTAAGTTTTAACTTCCCGAAAGCAATTACCg                                                                                                                                                                                    >  1:683557/1‑238 (MQ=255)
                                                            ccaTGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCCCCGGACATTTCGCGCCGAACAGAGTTAAGGTTGAACTTTCCGGAAAGCATTACCGCCTCTTCCtcg                                                                                                                                                                         >  1:667943/1‑237 (MQ=255)
                                                               tGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGCGGAACCTTTCCCGCGCACCGCGGGTTACTTTTAACTTTCCGAGAGCCATTACACCCCCTTcccttgcc                                                                                                                                                                      >  1:38010/1‑233 (MQ=255)
                                                                gcggctCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCGCACGCGGATGGCTGACACGTGCCTTCCGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCCGTTTCGCGGGACATTTCGCCCGCACCGCGGGTAAGATTAAACTTACACAAACACAATACCGCCCCTTACtggacag                                                                                                                                                                     >  2:323684/1‑234 (MQ=255)
                                                                              aaaTCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGCGGAAACTTTCCCGCGTACCCAGGGTACGt                                                                                                                                                                                                              >  1:97779/1‑185 (MQ=255)
                                                                                 tCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGCGGAACATTTcgcgc                                                                                                                                                                                                                             <  1:570932/167‑1 (MQ=255)
                                                                                     cACGTCGATGGAGCGCTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGGGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTTGCGGGACATTTCCCGCGTACCGCGGTTAAGTTTGGCCTTACCCAAAGCAATTACCCCCCCTCCCTGCGTCGGTggccccgcccggtcgttc                                                                                                                                                 >  2:370841/1‑221 (MQ=255)
                                                                                          tgATGGGGCTTTCCTTCGGGGAATATACGCCACCGCGGGAAGGTTTCCTCAGCTTACCGGGGTAGGGCGCCCCGCGCGGAGGGCTGACCGGGGCCTCAGTCACCCCAACCTCCCCTGTGGTGGGCGGCACCGGCTGTTCTTCGGGGACCATTCCGCGCGTACCGAGGTTAAGTTTGAACTTCCCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTga                                                                                                                                           <  2:237053/238‑1 (MQ=255)
                                                                                          ctgtGGCGCTTTCCGTTGGGGAATATTCCCGCCCGCGGGCAAGGTCCATCAGCTTATCGGGGGAAGGCGGCTCACGGGGTGGGCGGACCAGGGCCTTCAGTCCCGCAAACCCACCGCTGGTGGGCGGCCGCGGCCTGTCTTCGCGGAACTTTTCGCCCGTACCGGGGTTAAGTTTGAACTTCCCGAAACCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTga                                                                                                                                           <  2:446044/236‑1 (MQ=255)
                                                                                             tGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGCGGAACATTTCGCCCGTACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGGTCGTTCGCCCgacg                                                                                                                                         >  1:587357/1‑238 (MQ=255)
                                                                                                    ttCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGCGGAACATTTCGCGCGTACCCAGGGTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTTCTGTGACGGTGCGCCCGGCAGATCGTTCGCCTGACGGCgcagc                                                                                                                                  >  1:68651/1‑238 (MQ=255)
                                                                                                    ttCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGCGGAACATTTCGCGCGTACCAAGGGTAAGTTTGAAATTACCGAAAGCAATTACCGCCCCTTCCTGTGACGGGGCGCCTGGCAGTTCGTTTGCCTgccgaacacga                                                                                                                                  >  1:583620/1‑232 (MQ=255)
                                                                                                          tGCGGAATATTCACGCCCCCGGGCAAGGTTCATCAGCTTAGCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGGGGAACATTTCGCGCGTACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCAc                                                                                                                           <  1:7093/239‑1 (MQ=255)
                                                                                                                tttttccctcccccggcaaaGGTTCACCCTCTTATCGCGGGGGGCGGCCCCCCGCGGGTGGCTACCCAGTGCCTTCAGTCCCGCAACCCCCCCGCTGGTGAGCGGCACCGGCCCGTTTTCCCGTACCATTTCGCGCGTACCGAGTTTAGGTTTCACCTTCCCGAACGAAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATa                                                                                                                      <  2:68651/220‑1 (MQ=255)
                                                                                                                        gACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCCCGGAACATTTCGCGCGTACCGAGGTTAAGGTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACCGGGCGCCTGGCACTTCGTTCCCCTTACGACGCCCCACCCCCCGGCAACCGGccccc                                                                                                             >  1:211509/1‑236 (MQ=255)
                                                                                                                                         atccGCTTATCGGGGGAGGGCGGCTCACGCGGGTGGTCGACCAGTGCCTTCAGTCCCGCAAACTCACCGCGGGGGAGCGGCAGCGGCTCGTCTTCGCGGAACATTTCGCGCGTACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTaaa                                                                                           <  1:570641/239‑1 (MQ=255)
                                                                                                                                              cTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTTGCGGAACATTTCGCGCGTACCGAGGTTAAGTTTTAACTTACCGAAAGCAATTACCGCCTCTTCCTTTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCCCGGATACGGGCCAGCCGTTTCCGCGGGGTACACGGtt                                                                                      >  1:731042/1‑239 (MQ=255)
                                                                                                                                               ttATCGGGGAAGGGCGGCCCCCGGTGATGGCTGCCCAGTGCCTTCAGTACCGAAACCCCACCGCTGGTGAGCGGCATCGGCTCGTCTCCGCGGAACATTGCGCGCGTACCGGGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGttt                                                                                     <  1:598678/239‑1 (MQ=255)
                                                                                                                                               taaTCGCGGGAGCGCGGTCCACGCGGATGGCTGACCGGTCCCTTCATTACCGCAAACTCACCGGTGGTGAGCGGTATCGGCTCGTCTTCGCGGAACATTTCGCGCGTACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGtt                                                                                      <  1:832323/236‑1 (MQ=255)
                                                                                                                                                   cgcgGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGCGGAACATTTCGCGCGTACCGAGGGTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGGGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCCGCACCGCCCGGGTACGGGCCAGCAGTTCCCCCCGGGTAAACGGTTTTggg                                                                                 >  1:546816/1‑238 (MQ=255)
                                                                                                                                                                             tgttgtaaaagacacgaGTCCCGCAACCCCCCCGGTGTGGGGCGGCCTCGGCTCTCCTCCGGGGACAGTTCCCGGCGGACCGGTTTTAAGTTTGAACTCACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCa                                                        <  2:562484/223‑1 (MQ=255)
                                                                                                                                                                                              agtcccgaaacccccccGTTGGTGGGCGGCACCGGCTCGTCTCCGCGTAACTTTCCGGGCGTACCGAGGTTAAGTTTGAACTTCCCGAAAGAAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTc                                        <  1:370841/224‑1 (MQ=255)
                                                                                                                                                                                                 aCCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGCGGGACATTTCGCGCGGACCGAGGGTAAGTTTGGACTTACCGGAAGCAATTACCGCCTCTTCCTGGTACGGTGCGCCTGGCAGGTCGTTTGCCCTACGCCGCAGCACCCCCCGGGTACGGGCCAGCAGGTTCCGCGGGTTAAAACGTTTTGGGATGTAGTGGGCAACCCCCAACACCACGCCTACAAAAAGGTcccc                                     >  2:25205/1‑237 (MQ=255)
                                                                                                                                                                                                     cAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGCGGAACATTTCGCGCGTACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACCCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAAAGTAGTCGTCAGCGCCAATCTCCCGGCCTACGATACGGTCCAcctct                                 >  1:17840/1‑239 (MQ=255)
                                                                                                                                                                                                          tCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGCGGAACATTTCGCGCGTACCGAGGTTAAGTTTGAACTTACCGAAAGCCATTACCGCCTCTTCCCTTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCCCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGGTTTGGAATGTAGTCGTCAGCGCCAACCTCCAGGCCCACGATActgcttcttttacaca                             >  1:562484/1‑223 (MQ=255)
                                                                                                                                                                                                             ccGCTGGTGAGCGGCATCGGCTCGTCTTCGCGGGACATTTCGCCCGTACCCGGGGTAAGTTTTGACTTACCCCAAGCAATTACCCCCCCTTCCCTTTGCGGGGCCCCGTGCAGTTCGTTCGCCCGCTGCCCCCGCACCCCACGCGTCCGGGCGCGCAGTTTCCCGCGGGTACAAGCGTTTTGGATGGTCTTGTCCACGCCCATCCCCCGGCCTCCGTAACGGGCCACTCTTTCCCCtt                          >  2:346148/1‑238 (MQ=255)
                                                                                                                                                                                                                tgggTGAGCGCAACTGGCTCGTTGTCGCGGAACTTTTCGCGGGTCCCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGc                      <  1:444608/237‑1 (MQ=255)
                                                                                                                                                                                                                     ggaggggtctgggaccttcctCGCGGAACTTTGCGCGCGTACCGGGGTTAAGTTGAAATTTACCAAAAGAATTTGCCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGt                   <  1:198062/218‑1 (MQ=255)
                                                                                                                                                                                                                      agcggcagcggcTCGGTTTCGGGGAACTTTTCGCCGCAACGGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCa                 <  1:611833/238‑1 (MQ=255)
                                                                                                                                                                                                                      aGCGGCATCGGCTCGTCTTCGCGGAACATTTCGCGCGTAcc                                                                                                                                                                                                                        <  2:777832/41‑1 (MQ=255)
                                                                                                                                                                                                                      aGCGGCATCGGCTCGTCTTCCCGGAACATTTCGCGCGTAcc                                                                                                                                                                                                                        >  1:777832/1‑41 (MQ=255)
                                                                                                                                                                                                                        cGGCATCGGCTCGTCTTCGCGGAACATTTCGCGCGTAACGAGGTTAAGTTTTAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGGGCGCCTGGCAGTTCGTTCGCCTGACGACCCAGCACCCCACGGGTAAGGGCCAGCAGTTCACGCGGGGTAAACGGGTTTGGAATGTAGACGTCAGCGCCACACTCCAGGCCTACCATACGGGCCCATTCTTCCCCCTTCCCCCCCAccc              >  2:884916/1‑238 (MQ=255)
                                                                                                                                                                                                                                gCTCGTCTGCACGAAAGTTTCCGGGGGTCCGGAGTTAAAGTTTGAACTTAGCAAAGGAAATTACCGGCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGAt       <  1:785536/238‑1 (MQ=255)
                                                                                                                                                                                                                                     gTCTCCGCGGAACTTTTCGGGCGTACCGAGTTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCa  <  1:688300/239‑1 (MQ=255)
                                                                                                                                                                                                                                      tCTTGGCGAAAATTTTCGCGCGTCCGGAGTTTAAGTTTGAACTTACCGAAAGAAATCACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCa  <  1:38838/238‑1 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                            
AGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGCGGAACATTTCGCGCGTACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCA  >  NC_000913/3535889‑3536353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: