Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 417,593 A→G 17.9% E151G (GAG→GGG)  phoB → response regulator in two‑component regulatory system with PhoR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913417,5930AG17.9% 107.0 / 3.5 56E151G (GAG→GGG) phoBresponse regulator in two‑component regulatory system with PhoR
Reads supporting (aligned to +/- strand):  ref base A (11/33);  new base G (2/10);  total (13/43)
Fisher's exact test for biased strand distribution p-value = 7.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCG  >  NC_000913/417356‑417824
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                        
cGATGCCCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGGGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAagag                                                                                                                                                                                                                                         <  1:207005/239‑1 (MQ=255)
 ggtggcccGGGATATTCCGGTGGTGATTTTGGCCGCCAGAGGGAAAGAAGAATAACGCGTGCGGGGCATTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAagag                                                                                                                                                                                                                                         <  1:909122/233‑1 (MQ=255)
 ggagACCGGGGATATTCCAGTGGGTATGTTGCCCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGTCCTTGAAACCGGCGAGGATGCATATAACACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAagag                                                                                                                                                                                                                                         <  1:253640/235‑1 (MQ=255)
   tGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAAGGGGGATTGAAGTGCAGGGATTAAGTCTCGACCCGCCAACTCACCGGGGGATGGG‑‑GGGCGAagaggc                                                                                                                                                                                                                                       >  1:301483/1‑236 (MQ=255)
                     tggtgATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCCCCGAGGGATGGC‑‑GGGGGGAAAGCCGCCGGGGGTGGGGGCGACa                                                                                                                                                                                                                    >  1:326857/1‑239 (MQ=255)
                     tggtgATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCCCGGCCCGCCATCTCACCGGGGGATGGC‑‑GGGGGAAGGGCCCCCGGGAACGGGGCCggcc                                                                                                                                                                                                                    >  1:20265/1‑236 (MQ=255)
                       gtgATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGCCCCGACCCGACATCTCACCGAGGGAGTGC‑‑GGGGGAAGAGCCGCTGGGGATGGGGCCGCCAGa                                                                                                                                                                                                                  >  1:735651/1‑239 (MQ=255)
                          aTGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGGAGAGACGCTGGAGGTGGGGCGGGCAGAAtt                                                                                                                                                                                                               >  1:759216/1‑239 (MQ=255)
                          aTGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGGCTCGACCCGACATCCCCCCGAGTGATGGC‑‑GGGGGAAAAGACGCCGGGGCAGGGGCCgcccagatt                                                                                                                                                                                                               >  1:670985/1‑231 (MQ=255)
                                     cAGGGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTaaa                                                                                                                                                                                                           <  1:832335/232‑1 (MQ=255)
                                              agaagaagaTCGGGGGGGCGGCCTTGAAACCGGCGGGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACtttttt                                                                                                                                                                                            <  1:866910/238‑1 (MQ=255)
                                              agaagaagaTCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGGAGAGGGGATTGGGATGCAGGGATTAAGTCTCGACCCGACATCCCACCGAGGGGTGGCGGGGGGGAGGAGCCCGGGGAGAGGGGGCGCCAAGAATAAAAACTGTGCACtttttt                                                                                                                                                                                            >  2:685425/1‑239 (MQ=255)
                                               gaagaagaTCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGAGTAAGTCTCTACCCGCCATCTCACCGGGTGATGGG‑‑GGGGGGAGAGACGCGGGGGAGGGGGGGGACCGAAATTAAAaagctgtccccttgtc                                                                                                                                                                                           >  2:832335/1‑228 (MQ=255)
                                                 agaagaTCGGGGGCCGGGCTTTTAAACCGTCGCGGATGACTATATCCCCAAGCCGTTTTCGGCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAAGGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCGCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGa                                                                                                                                                                                        <  2:740215/239‑1 (MQ=255)
                                                          cgTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGGGATGGC‑‑GGGCGAAAAGCCGCTGGGGATGGGGCCGACCGAATTTTAACTGGTTCACCTTTTTTTGACGCATcccg                                                                                                                                                                               >  1:82253/1‑237 (MQ=255)
                                                           gTGCGCGGCCTTGAAACGGGCGGGGATGGCTAAATCTCGAAGCCGTTGTCGACGAAGGAGCTGGTGGCGCGAAGCAAAGCGGTAATGCGCCGTATTTCGCCAAGGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGGCATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTg                                                                                                                                                                               <  1:404518/238‑1 (MQ=255)
                                                             gcgcGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATAAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGGGGCAGGGGGGGTTGGGATGACGGGGATAAGGCTCGCCACCACACCTCACCGAGGGATGGGG‑‑GGGGGGAGGGGCCCGGGGAATGGGGCGAGCAAAATTTAAACTAGCGCACTTTTTTTTGACGAATACTgcg                                                                                                                                                                             >  2:859523/1‑237 (MQ=255)
                                                                               gcgggaggaCTTTACCCCAAAGCCGTTTTCGCGGAAGGGGGTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGagc                                                                                                                                                          <  2:872142/236‑1 (MQ=255)
                                                                                ggcgggtaattatcccccATGCCGTTTTCGTTGGAGGAGTCGGTGGGCCGAAATAAAGGGATAAGGCGCCGTTTGTCGCCAAGGGCGGGGGAAGAGGTGATTGGGATGCAGGGATTAATGCTCGGCCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGagc                                                                                                                                                          <  2:100630/222‑1 (MQ=255)
                                                                                cgcgattgatagccccccAAGCCGGTTTCGCGGAAGGGGTGGTTGGCGCGAAGCAAAGTGGTAATGCCCCGTACTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACAGCTCACCGGGTGATGGC‑‑GGGCGAAGGGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGagc                                                                                                                                                          <  2:495183/222‑1 (MQ=255)
                                                                                 cgGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGagca                                                                                                                                                         <  1:735150/238‑1 (MQ=255)
                                                                                         tataTCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAg                                                                                                                                                                             <  1:601231/210‑1 (MQ=255)
                                                                                          gtatCCCCAGGCCGTTTTCGCCGAAGGGGCTGGGGGCGGAAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAAc                                                                                                                                               <  1:309901/238‑1 (MQ=255)
                                                                                                  taggctttttCGGCGGAGGGGCGGGTGGGGGGAATAAAATGTGTAATGGGCGGGATTTGGCGAATGGCGGTGGAAGAGGTGATGGAGGTGCAGGGATTAAGTCTCGCCCCGACATCTCACCGAGTGAAGGC‑‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCAGCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTg                                                                                                                                       <  2:75480/233‑1 (MQ=255)
                                                                                                  aaGGCGTTTTCGCCGAGGGGGTTGGGGGCGGGAATCAAAGGGGTAATGCGCCGTATTTCGCCAATGGGGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCGCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTg                                                                                                                                       <  2:737083/239‑1 (MQ=255)
                                                                                                  aaGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTg                                                                                                                                       <  1:582266/239‑1 (MQ=255)
                                                                                                  aaGCCGTTTTCGCCGAAGGAGCTGGTGGAGGGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTg                                                                                                                                       <  1:820399/239‑1 (MQ=255)
                                                                                                            ccccGAAGGGGCGGGTGGCGCGAATCAAAGCGGTAAGGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGGGATGCAGGGATTAAGGCTCGACCCGACATCTCACCGATTGATGGC‑‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAAc                                                                                                                             <  2:640295/237‑1 (MQ=255)
                                                                                                            cGCCGAAGGAGCTGGTGGCGCGAATCAAAGGGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAAc                                                                                                                             <  1:561123/239‑1 (MQ=255)
                                                                                                            cGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAAc                                                                                                                             <  1:199063/239‑1 (MQ=255)
                                                                                                            cGCCGAAGGAGCTGGTGGCGCGAAACAAAGCGGTAAGGCGCCTGATTTCGGCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAAc                                                                                                                             <  1:728360/239‑1 (MQ=255)
                                                                                                               cGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGGAGAGCCCCTGGGGGTGGGGGCGACAGAATTTTAACTTCTTCACTTTTTTTTGGCGCCTCCTGAGCGCGTGTACAACCGCGAGCCGCTGTTAAAACACCGCTGGGGGACaaaaggt                                                                                                                          >  1:228829/1‑235 (MQ=255)
                                                                                                                      gCTGGGGGCGCGAACGAAAGCGGTAATGCGCCTTATTTCGCCAATGGCGGTGGGAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCATCTGTTAAACCGCGTCTGGGGAGCGAACGTGTAtgtg                                                                                                                    <  1:136415/238‑1 (MQ=255)
                                                                                                                         gggggcggaaaTCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGaa                                                                                                                 <  1:541016/229‑1 (MQ=255)
                                                                                                                                cgaaataaaGGGGTAATGCGCCTTATTTCGCCAATGGCGGTGGAAGAGGTGATTGGGATGCAGGGATTAAGTCCCGCCCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCAc                                                                                                         <  2:760398/233‑1 (MQ=255)
                                                                                                                                cgAATCAAGGCGGTAATGCGCCGTATTTCGCCAATGGCTGTGGAAGAGGTGATGGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCa                                                                                                          <  1:375412/238‑1 (MQ=255)
                                                                                                                                caaaTCAAGGCGGGAATGCGCCGTATGTCGGCAAGGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCa                                                                                                          <  1:859523/236‑1 (MQ=255)
                                                                                                                                 ggatcaagggggttgtgccgcGTATTTCGGCAATGGCGGTAGAAGGGGGGGTTGAGTTGCAGGGATTAAGGCTAGACCCGACATCTCACCGGGTGATGGC‑‑GGGCGAAGAGCCGCTGGAGATTGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGATCTAACGTGTATGTGGAAGACCGCAc                                                                                                         <  2:343755/218‑1 (MQ=255)
                                                                                                                                    tCAAAGCGGTATTGCGCTGTTTTTCGCCAATGGGGGTGGAAGAGGTGATTGGGAGGCAGGGATTAGGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTc                                                                                                     <  2:326857/239‑1 (MQ=255)
                                                                                                                                          cGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACg                                                                                                                                                                                      <  1:553036/152‑1 (MQ=255)
                                                                                                                                                        ttttCGCCAAGGGGGGGGGAAGGGGTGATTGGGAGGCGGGGATTAAGTCCCGACCCGAAATCTCACCGAGTGATGGC‑‑GGGCGAAGGGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCt                                                                                <  2:670985/238‑1 (MQ=255)
                                                                                                                                                            cGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCCGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAAACCGCACGGTCGATGTCCAACTTCGTCGCCTGCGt                                                                            >  1:106062/1‑239 (MQ=255)
                                                                                                                                                             gCCAAGGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTa                                                                           <  1:623050/239‑1 (MQ=255)
                                                                                                                                                                             tgggtttttgtgggggaGGGATTAGGGCTCGGCCCGACATTTCACCGAGTGATGGC‑‑GGGCGAAGGGCCGCTGGAGATGGGGCGGACAGAATTTAAACTGCTGCACTTTTTTAGGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCcg                                                            <  2:120140/222‑1 (MQ=255)
                                                                                                                                                                                          tGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCGCTGGAGGTGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCCGGGCGCGTGTAAAGCCCGGAGCAGCCTTTAAAACACGTCTGGGGGACTAACGGGAATGGGGAAAGCCGCCCGGGCGGTGGCCACATTCGGCCGCCTCGGAAACCACTGGACCCCGGCGGGGAGGAcccc                                              >  2:602620/1‑237 (MQ=255)
                                                                                                                                                                                          ggaggggtttaatgtgcGACCCGACATCTCACCGAGGGATGGC‑‑GGGGGAAGGGCCGCTGGAGATGGGGCCGAACGAATTTAAACGGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGGGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGc                                              <  2:45330/223‑1 (MQ=255)
                                                                                                                                                                                           gCAGGGATTAAGTCCTGACCCGGCATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCGGTGGAAATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGc                                              <  1:131365/238‑1 (MQ=255)
                                                                                                                                                                                            gggggAATAAGGCGCGACCCGCCCTCTCACCGGGTGATGGC‑‑GGGGGAAGAGCCGCTGGGGATGGGGGCGACAGAATTTAAACTGCTGCACTTTTTTATGACGGATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCa                                             <  2:735651/236‑1 (MQ=255)
                                                                                                                                                                                             gggtgttAAGTCCCGCCCCGCCATCTCGCGGAGTGATGGG‑‑GGGAGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCAt                                            <  2:265684/233‑1 (MQ=255)
                                                                                                                                                                                             gggggttaggttccgccccGACATCTCAACGGTGGAGGGG‑‑CGGCGAAGGGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGGTGCACTGTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCAt                                            <  2:822790/222‑1 (MQ=255)
                                                                                                                                                                                             aGGGATTAAGTCTCGACCCGACATCTCACCGGGTGATGGG‑‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCAt                                            <  1:864255/238‑1 (MQ=255)
                                                                                                                                                                                                           ggcccGGCATCCTACCGGGTGATGGC‑‑GGGCGAAGGGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTgcg                             <  1:300695/237‑1 (MQ=255)
                                                                                                                                                                                                           gACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAAAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTgcg                             <  1:152030/239‑1 (MQ=255)
                                                                                                                                                                                                            gcggcgggcccgcccggatgtagggc‑‑gggcgAGGGGCTGCGGGGGATGGGGGCGGCAGAATTTAACCTGGAGCCTTTTTTTGTGCCGGATCCTGAGCACGTGTACAGCGGGGAGCAGGTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGGGCCCGGCGGGCATGACCGCATGGTGCAGACCGTgcg                             <  2:213111/216‑1 (MQ=255)
                                                                                                                                                                                                                gccctcccACCGGGTGGTGGC‑‑GGGCGAAGGGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCCCTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTa                        <  2:228829/232‑1 (MQ=255)
                                                                                                                                                                                                                gACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTa                        <  1:685425/239‑1 (MQ=255)
                                                                                                                                                                                                                              tGATGGC‑‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCg                                                                                                    <  2:14349/150‑1 (MQ=255)
                                                                                                                                                                                                                              tGATGGC‑‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCg                                                                                                    >  1:14349/1‑150 (MQ=255)
                                                                                                                                                                                                                                        gggggAAGGGCCGGTGGGGATGGGGCCGATAGAATTTAAACTGCTGCACTTTTTTATGCCGCATCCTGAGCGGGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAAcc    <  2:82253/238‑1 (MQ=255)
                                                                                                                                                                                                                                          gcgAAGGGCCGCTGGAGGTTGGGCCTACAGAATTTAAACTGCTGGACTTTGTTATGACGCATCCTGAGCGCGTGTCCAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCg  <  2:759216/238‑1 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                        
CGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCG  >  NC_000913/417356‑417824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: