| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,824,731 | T→G | 22.1% | V73G (GTG→GGG) | trmH → | tRNA mG18‑2'‑O‑methyltransferase, SAM‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,824,731 | 0 | T | G | 22.1% | 142.7 / 2.7 | 70 | V73G (GTG→GGG) | trmH | tRNA mG18‑2'‑O‑methyltransferase, SAM‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (11/39); new base G (16/3); total (28/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.40e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTGGCGGATCAGGACATCATCATTCCGATGATCGGCATGGTGCAGTCGCTGAATGTTTCC > NC_000913/3824507‑3824966 | aTGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGAAGGAAA‑AGCTGGGTACAG‑‑GGGAAAAAACACCGc > 1:435017/1‑238 (MQ=255) ttATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGGTGTAAA‑AGCTGGGGACCA‑‑GGGAAAAACCAACGCAcc > 1:300706/1‑236 (MQ=255) tATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTCGTAAA‑AGCCGGGTACAG‑‑GGGGAAAAACACCCCACCATTg > 1:505385/1‑239 (MQ=255) aaaccGTTTTGCCCGTATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTTTGCATGGAGCAGTTCCCAAAACCTCATAACGTTTCTGCGATGATTCGTACCGCAGATGCCGTTGGCGTACACGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACCCACCGCACCATTGGCGATGCCg < 2:897345/235‑1 (MQ=255) gtttggCACGCATCTGGGAAATGCCCGCCGGGCGTCAGCCTGATCTGCCCGTCTGATTGGCGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATCCGTCCCGCAGATGCCGTTGGCGTACAGGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCt < 2:680441/234‑1 (MQ=255) tacgcgcgagtgtgggAAATTCTGGCAAGGCGGCCGCCTGATCTTGCCGTCTGCATGGGAGAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGGCGTTGGCGTACATGAAGTTCACGCCGTCCGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAGGTGTGAAAACACACCGCACCATTGGCGATGCCGTCGCt < 2:622313/224‑1 (MQ=255) tATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGGAGTAACGTCCGGGGCACGG‑‑GGAAAAACACCCCGCACAATGGGGGAtccgggcgtca > 1:558235/1‑229 (MQ=255) gcGCCTCTGCGAAAGGCTCCCCGGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTTTTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCa < 2:435017/238‑1 (MQ=255) ccGCACCGGCAAAATTCCCGCCGGGGGGAAGCCTGATGTGCCCGTTTGGATGGGGCAGGTCCACAACCCTCATAACGTTTGTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCa < 2:834777/238‑1 (MQ=255) cATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGGACAG‑‑GGGAAAAACAACCGCCCCATTGGCGAGGCCGGCGCTCATCCCAAAg > 1:523301/1‑239 (MQ=255) aTCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAACTGCGGGTGGGACA‑AGCTGGGTACAG‑‑GGGAAAAAACCCCCACCCACTGGCGAAGACGTCGCTCacaccaaaag > 2:19619/1‑230 (MQ=255) ttGCGAAATTCCCGCCAGGCGGCAGCCTTATCTGACCTTCTGGATGGAGCAGGTCCACAAACCTCATTACGTTTCTGCGATTATGCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGcc < 2:588905/238‑1 (MQ=255) ccgagaatggCTCGCCGGGCGGCGGGTTGATTCGACCGTCGGCATGGAGCAGGGCCACAAACCGAATACCGTTTCTGCGGTTATTCGTACCGCAGATGCCGTTTGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGc < 2:183429/229‑1 (MQ=255) cTGGGAAATGGCCGCCAGGGGGAAGCCGTATCTGACCGTTTGCATGGAGCAGGCCCACAACCCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGcc < 2:523301/239‑1 (MQ=255) cTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTAAAG‑‑GGGTAAAAAACCCGCACCATTGGGGATGACGTCGCCCATCTCCAAAGcc > 1:177266/1‑239 (MQ=255) tGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGGTGCCGTTCGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGGGGTACA‑AGCTCGGTCCCA‑‑GCGAAAAACAACCGCCCCCTTGGGGCTGCCGCGCCCCATCTCaaaaagcaaggcgtg > 2:340221/1‑227 (MQ=255) gCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GGGGAAACACACCGCACCATTGGCGCTTCCGTCGCTCATCTCAAAGGCCCGGGCaatcaaaa > 1:817889/1‑232 (MQ=255) ccTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGAACCATGGCTTCGGCAGCGGCGGGTCGTAAC‑AGCGGGGTTCAG‑‑GGGGAAAAACACCCCCCCCATTGTGATGCCCTCGCCCCCCTCAACAGCCAGGGGCCTCCG‑ATTC‑TGTCAAcccc > 2:529694/1‑238 (MQ=255) aTCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCtt < 1:909815/239‑1 (MQ=255) ccttctgctggggagaGGTCCACAACCTCCATAACTTTTCTGGGATTATTGGTCCCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGa < 2:89323/223‑1 (MQ=255) cttcctGGGGCCGGTCCCCAAACCCTATAACGTTTCGGGGATTTTTCGTCCCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACg < 2:817889/234‑1 (MQ=255) ctcccttggtctggTTCACAAGCCTTATAACGTTTCTGCGATTATTCGGACCGCGGATGCCTTTGGCGTCCATGAAGTTCAGGCCGTCTGGGCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGGACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACg < 2:847786/227‑1 (MQ=255) cTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGGGGTAAC‑ACCGGGGGACCG‑‑GGGAAAACACACCGCCCCCCAGGCGAGGGCGTGTCTCCACTCAAAGGCCAGGGACCGCAGAATTC‑T‑TCAACCCCCCTTTCTggttaa > 2:154430/1‑233 (MQ=255) cTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGGAGTAAC‑AGCTGGGGTCAG‑‑GTGAAAAAACACCGCACCCTTGGCGATGCCGTGCCTCCACTCAAAAGCCAGGGCAAGCAG‑ATTC‑TGGCAACACACATTTCTGTTaaa > 1:14805/1‑237 (MQ=255) cAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACCAACCCCACCATTGGCGAGTCCGCGGCTCATCTCAAAGGCCAGGGCATGCCA‑ATTC‑TGGCAACCCATCTTTATGGTAACGCTG‑T‑CGAtt > 1:42770/1‑238 (MQ=255) caacccctaaaaCGTTTCGCCGATTATTCGTACCGCGGTAGCCGGTTGGGTACAGGAAGTTCACGCGGTCGGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGaaa < 2:794056/229‑1 (MQ=255) caAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCGGGGAACAG‑‑GGGAAAACACCCCCCACCCTAGGCGTTGCCGCCGCGCATTTCAAAGGCCCGGGCGTACAG‑AATT‑AGGGACACCATCTTTTTGCTAACGCCG‑T‑CGCTGTcccgcaaa > 2:884071/1‑233 (MQ=255) cTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGGAGTAAC‑AGCTGGGGCAAG‑‑GGGAAAACACACCCCACCATTGGCGATGCCGTCGCTCATCTCCAAAGCCAGGGCATGCAG‑ATTC‑TCGCAAACCACCCTTCTTATAACCCCG‑T‑GGAGTTTCGCGGAAATGAt > 2:481419/1‑239 (MQ=255) tAACTTTTTTGCCATTTTTCGGTCCGCGGATGCCGTTGGCGTACATGAAGTTCCCGCCGTCTGGCCCGGGAGCCGGATGCGGACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTaca < 2:119051/239‑1 (MQ=255) tccgCGATTACTCGTACCGCATATGCCGTTGGGGTACATGAAGTTCACGCCGGCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGccc < 1:612198/236‑1 (MQ=255) tGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGGAGTAAC‑CGCTGGGTACAG‑‑GGGAAAACACACCGCACCACTAGCGATGCCGCCGCTCATCTCCAAAGCCAGGGCGCGCCG‑CTTC‑TGGCCACCCACCTTTCTGTTTACACTGTT‑CGATTACCGCAAAAtatataaaccccccccc > 2:384830/1‑223 (MQ=255) ttattCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGGAGTAAC‑AGCCGGGTGCAG‑‑GGGAAAACACACCCCCCCCCTGGGGATGCCCTCGCTCCACTCAAAGGCCAGCGCCTGCAA‑ATTCGTGACACCCCA‑CTTTTTCATAACGCTT‑T‑CCGATTCTCCCAAATTGTTTaccaccccccccccac > 2:487326/1‑224 (MQ=255) attCGTAACGCAGATGCCTTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCAt < 1:529694/239‑1 (MQ=255) cGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGGAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGGGATGCCGTCGCTCATCTCACAGGCCAGGGGCTGCGA‑ATTC‑TGGCAACCCATCTTTCTG‑TAAAACCG‑TGCGATTTCCCCGCAATTTATTACACACcccccaaccgacttttggggg > 2:328856/1‑222 (MQ=255) ggCGTACATGAAGTTCCCGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAAc < 1:481419/239‑1 (MQ=255) ggCGTACATGAAGTTCCCGCCGTCTGGCCTGGTAGCCGCATGCGCACCAGGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAAc < 1:791276/239‑1 (MQ=255) cGTACATGAAGGTCACGGCGGCTGGCCTGGTAGCCGCATGCGGACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACCCCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACg < 1:411666/238‑1 (MQ=255) tGAAGTTCCCGGCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑Ca < 1:19619/239‑1 (MQ=255) atttCCCGCCGTCTGGCCGGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGc < 1:566624/237‑1 (MQ=255) gtccACGCCGGCTGGCCTGTTAGCCGCATGCGAACCAGGGCTTCGGCGGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGa < 2:851264/208‑1 (MQ=255) tccccccctttGGCCGGGTAGCCGGAGGCGGACCAGGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAgg < 2:847275/229‑1 (MQ=255) cGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCGGGGTACAG‑‑GGGGAAACACACCCCCCCATTGGCGAATCCGTCGCTCCTCTTAAAAGCCAGGGCCAGCAG‑ATTT‑TGGCAACCCCTCTTTTTGTTAACCCTG‑A‑CGATTTCCGCGAAATTGATTTTACTCTCCCCACCCGCACTTTTTTGGGGCGGTGGAAAAAggggaaaaagccgagga > 2:692898/1‑225 (MQ=255) cTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GGGAAAACACACCCCACCCTTGGCGATGCCGTCGCTCATCTCAAAAGCCCGGGCATCCCG‑ATTC‑TGGCAACCCCCCTTTTTCTTAACGCTG‑G‑TGCGTTCCCCGCAACTGGTTACACTCCCCCGACCTTCATTTTTCTGGGGCACGAGAAAAACGGCAT‑ACCGCCGGGAGCA‑ATg > 2:195580/1‑238 (MQ=255) gCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGaa < 1:422672/166‑1 (MQ=255) tgtgcgccccagcgcgaCCCTGGCTTCGGCAGCGGCGGGTAGTAAG‑TGCGGGGTACCG‑‑GTTAAAACCCACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTgg < 2:888807/223‑1 (MQ=255) tGGTAGCCGCATGCGCCCCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTgg < 1:556948/239‑1 (MQ=255) tGGTAGCCGCATGCGACCAATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGGTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTgg < 1:884071/239‑1 (MQ=255) ggttggCCGCGTGCGCACCATGGCTTTGGGCGCGGGGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACCCCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCAGCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTgg < 2:602548/234‑1 (MQ=255) cgcgatacgcgCCTTGGCTTCGGCAGGGGCGGGTAGTAAC‑CGCGGGGTACAG‑‑GGGAAAACACGCCGCCACATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTGGCGGAt < 2:177266/231‑1 (MQ=255) ccccgggtgcgcaaTGGCTTCGGGAGGGGCGGGTAGTAGC‑AGCTGGGTACAG‑‑GGGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTTCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTGGCGGATc < 2:558235/226‑1 (MQ=255) ccGCATGCGCACCATGGCTTCGGCAGCGGCGGGGAGTAAC‑AGCCCGGTTCAC‑‑GTGAAAACACACCGCACCCTTTGCGGTGCCCTCCCTCATCCCAAAGGCCAAGGCCTACCG‑ATTC‑TTGCTACCCATCTTTCTCATAACGCTG‑T‑CGATTTACCCGAAATTTACTACACCCGCCCCACCTGCATCTTTCTGGGACACTGGAAAACAGCGATCCACCCAGAAACAA‑TTGGCCCTgcgggag > 2:412554/1‑233 (MQ=255) cGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTGGCGGATc < 1:381917/238‑1 (MQ=255) gccccccaggCTCCGGCAGGGGCGGGTAGTAAC‑AGCCGGGGACAG‑‑GTTAAAACCCACCGCACCATTGGCGATGCCGTCGCCCAACTAAAAGGCCAGGGCATGCAG‑ATTC‑TGGCATCCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTGGCGGATCAGGa < 1:340221/228‑1 (MQ=255) gccccATGGCTTCGGCAGGGGCGGGTTGTAAC‑AGGTGGGTACAG‑‑GGGAAAACACACCGCCCCATTGGCGATGCCGGCCCTCATCCCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCATGGCGGATCAGGAcat < 2:902502/235‑1 (MQ=255) aCCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACCCACCGCCCCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTGGCGGATCAGGAcatcat < 1:66221/239‑1 (MQ=255) ccATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGGTAAAGCTG‑T‑CGATTTCCGCGAAAATTATTAAACTCGCCCGACCTGCATTTTTGTGGGAACGGGGAAAACAGGCCT‑AACGCCCGAAGCACTTTGCCCTgcgggacagagaaacaaa > 2:585696/1‑222 (MQ=255) ggCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCa < 2:660758/80‑1 (MQ=255) ggCTTCGGCAGCGGCGGGTAGGAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCa > 1:660758/1‑80 (MQ=255) cTTCGGCAGGGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTGGCGGATCAGGACATCATCATTCCg < 1:277497/239‑1 (MQ=255) ttCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTTCAG‑‑GTAAAAACCCACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTGGCGGATCAGGACATCATCATTCCg < 1:692898/238‑1 (MQ=255) ttCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTGGCGGATCAGGACATCATCATTCCg < 1:293721/238‑1 (MQ=255) gccggggcggGTGGTAAC‑CGCTTGGTACCG‑‑GTGAAAACACACCGCCCCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTGGCGGATCAGGACATCATCATTCCgatgat < 2:300706/234‑1 (MQ=255) tgtacacgCTGGGTCCAG‑‑GTGAAAACCACCCGGCCCTTTGGCGATGCCGTCGCTCAGCGCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTGCGGATAACGCTG‑T‑CGAGTGCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGAGGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTGGCGGATCAGGACATCATCATTCCGATGATCGGCATGGTGCa < 2:405285/232‑1 (MQ=255) gtaAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTGGCGGATCAGGACATCATCATTCCGATGATCGGCATGGTGCa < 1:384830/238‑1 (MQ=255) gtaAC‑AGCTGGGGACAG‑‑GTGAAAACACACCGCTCCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTGGCGGATCAGGACATCATCATTCCGATGATCGGCATGGTGCa < 1:74814/238‑1 (MQ=255) cTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGCCAGGAGAAAACGGGCAT‑AACGCAGGAAGCA‑TTGGCCCTGCCGGATCAGGACATCCTCTTTCCGATGACCGGCCTGGTGCGGTccatga > 1:548275/1‑234 (MQ=255) cTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CCCCCCGGAAGCA‑TTGGCCCTGGCGGGTCAGGAAAACCGCATTCCGATTATCGGCCTGGGGCCAGCGCTGa > 2:74138/1‑239 (MQ=255) ggTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGCCAGTAGAAAACGGGCAT‑AACGCAGGAAGCA‑TTGGCCCTGGCGGATCAGGACATCATCATTCCGATGATCGGCATGGTGCAGTCGCTGAATg > 1:851858/1‑239 (MQ=255) ggTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAAAGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAAAGGGCAT‑CACGCAGAAACAC‑TTGGCCCCGGCGGATCAGAACATAACAATTCCGATAACCGGCATgggccatgcggtga > 2:728082/1‑224 (MQ=255) aG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTGGCGGATCAGGACATCATCATTCCGATGATCGGCATGGTGCAGTCGCTGAATGGTTcc > 1:561443/1‑239 (MQ=255) | ATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTGGCGGATCAGGACATCATCATTCCGATGATCGGCATGGTGCAGTCGCTGAATGTTTCC > NC_000913/3824507‑3824966 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |