| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,862,676 | G→C | 32.7% | pseudogene (927/1617 nt) | glvC ← | pseudogene, arbutin specific enzyme IIC component of PTS,enzyme, Transport of small molecules: Carbohydrates, organic acids, alcohols, PTS system, arbutin‑like IIB component, PTS system, arbutin‑like IIC component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,862,676 | 0 | G | C | 32.7% | 15.8 / 4.9 | 32 | pseudogene (927/1617 nt) | glvC | pseudogene, arbutin specific enzyme IIC component of PTS,enzyme, Transport of small molecules: Carbohydrates, organic acids, alcohols, PTS system, arbutin‑like IIB component, PTS system, arbutin‑like IIC component |
| Reads supporting (aligned to +/- strand): ref base G (6/14); new base C (3/6); total (12/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01 | |||||||||||
CGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCA‑CCA‑GCAT‑GGC‑GGTTAAGGT‑TGC‑GGG‑AATCAG‑C‑AAG‑CCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACG‑GCACCAAAGATTTTTGAGTT‑ACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGAAGTGGTGTAAAC > NC_000913/3862456‑3862914 | cgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCTTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCA‑CCA‑GCAT‑GGC‑GGCTTAGGT‑TTC‑GGG‑GATACG‑C‑CAA‑ACCCCCACtttttaccca > 1:51704/1‑233 (MQ=255) ggTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGGAAATTCCAGCGGTTCGGTAATTCCCA‑CCA‑GCAT‑GGC‑GGGTTATGT‑TGC‑GGG‑GAACAC‑A‑AAGACCCCCCACTTTTTcccactt > 1:471571/1‑235 (MQ=255) gTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCA‑CCA‑GCAT‑GGCGGGTTAGGGT‑TTG‑GGG‑AA‑CAGAC‑AAC‑CCCGCACCTTTTAccctgc > 1:875311/1‑234 (MQ=255) tCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCA‑CCA‑GCAT‑GGC‑GGGTAAGGT‑TTC‑GGG‑AAACCG‑C‑AAGCCCCGCCCCTTTTACCCCGTTTTCCgggcgcgcggggaa > 1:7231/1‑227 (MQ=255) tGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCA‑CCA‑GCAT‑GGC‑GGTTAAAGT‑TGC‑GGG‑GATCAG‑C‑CAA‑ACCGCCCCCTTTACCCCAATTTTCGTTGCGGGCGGGGAGTACAACg > 1:64891/1‑238 (MQ=255) tCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCA‑CCA‑GCAT‑GGCGGGTTAAGAT‑TAG‑GGG‑AA‑CAG‑CAAAG‑CCACCCCATTTTACCCGGTTTTCAGG‑CCGGCAGGGAAGAACATACCCAAAAAAAAGCCCACG‑Gc > 1:592437/1‑238 (MQ=255) tgtGTTCCCCCCCACAACAAAGAAATACTTTACGGGAAACAGTGGGGCCCCAAGCTCGGTGTGTCCCACAAACAGAAGCGGTAAAAGAAACGGGAGTGTAAATCCAAGCGGTTCGGTAATCCCCA‑CCA‑GCTG‑GGG‑GGTTAGGGT‑TGC‑GGA‑AATCAG‑C‑AAG‑CCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACG‑GCACCAAAGATTTTTg < 2:556607/237‑1 (MQ=255) aTACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCA‑CCA‑GCAT‑GGC‑GGTTAAAGG‑TTC‑GGG‑GATCAG‑C‑AAA‑CCCGCCCCCTTTTCCCCGTTTTTCGGGGCGGCAGTGGAGGACATAGCTAAAAAAATAGCCACG‑GCCCCACAGAATTTTTAGGT‑TTCGTGGAGGGGAAAAACg > 1:877167/1‑238 (MQ=255) tACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGGAATTCCCA‑CCA‑GCAT‑GGCGGGTTAAGGT‑TCG‑GGG‑GACGAG‑C‑AAC‑CCCCCCCCTTTTCCCCGATTTTCC‑GTGGGGGCGAGAAGGAAAACGCCTAAAAAAAGCCCCCGAGCCCCAAA‑AGTTTTTAGTG‑AACCTGGAGGGCaaaaacac > 2:158676/1‑234 (MQ=255) gggtgtCCCGAAAACAGAAACTGGAAAATGAACAGGAAGGTAAATCCCAGCGGTTCGGAAATGCCCC‑CCA‑GCAG‑GGG‑GGTTAAGGT‑TGG‑GGG‑AATCAG‑A‑AAG‑CCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACG‑GCACCAAAGATTTTTGAGTT‑ACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGgcgc < 1:436192/236‑1 (MQ=255) aaaCAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCA‑CCA‑GCAT‑GGC‑GGTTCAAGC‑TGCGGGG‑AGCCGG‑C‑AGC‑CCCCCCCCTTTTAACC‑AGTTTTCGGGGCGGCAGTGAAGTTCACTCCTAAAAAAAAGCCCCCC‑GGCCCCAAGAACTTTTAGTTGACCGTCCCGGCACAAACGCCCTCCCGGGACAAACAATTTCacggacccgcgcacaaaacaaac > 2:358178/1‑216 (MQ=255) aggaaagaaacgagAAGGTAAATCCCAGGGTTTCTATAATCCCCA‑CCA‑GCAT‑GGC‑GGTTAAGGT‑TGC‑GGG‑AACCAG‑C‑AAG‑CCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACG‑GCACCAAAGATTTTTGAGTT‑ACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGAtg < 1:158676/225‑1 (MQ=255) gaaagaaagaGGAAGGTAATTTCCAGGGGTCCGGAAACCCCCC‑CCA‑GCAG‑GGC‑GGTTAAGGT‑TGC‑GGA‑AACACG‑C‑AAG‑CCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACG‑GCACCAAAGATTTTTGAGTT‑ACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGAtgct < 1:491366/229‑1 (MQ=255) aaggaaagggAAGTAAAATCCCATGGGTTCGGTAATCCCCC‑CCA‑GCAG‑GGC‑GTTTAAGGT‑GGG‑GGG‑AACCAG‑C‑AAG‑CCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACG‑GCACCAAAGATTTTTGAGTT‑ACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTgcg < 1:668578/231‑1 (MQ=255) gaaaggaaaGATAAATCCCGGGGGTCGGGTAATCCCCC‑CCA‑G‑GT‑GGG‑GGTTTAGGG‑TGG‑GGGAAATCAG‑C‑AAG‑CCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACG‑GCACCAAAGATTTTTGAGTT‑ACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGcc < 1:189262/231‑1 (MQ=255) aagggaatttAAATTCC‑GGGGTTTGGTAAATCCCC‑CAACGCAG‑GGG‑TGTTAGGGT‑TGG‑GGA‑AATAGG‑C‑AAC‑CCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACG‑GCACCAAAGATTTTTGAGTT‑ACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAg < 1:821282/230‑1 (MQ=255) gAAGGAAAATTCCGGGGTTCCGGTAATCCCCA‑CAA‑GCTG‑GGC‑GTTTAAGGT‑GGC‑GGA‑AATCAG‑C‑AGC‑CCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACG‑GCACCAAAGATTTTTGAGTT‑ACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTa < 1:84242/237‑1 (MQ=255) ctcacagggtttgtgaaTACCCACCCA‑GCTG‑GGC‑GTTTAAGTT‑TGC‑GGG‑AATCAG‑C‑AAC‑CCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACG‑GCACCAAAGATTTTTGAGTT‑CCCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATg < 1:579891/224‑1 (MQ=255) cgTAATCCCCC‑ACA‑GGATGGGC‑GGTTAGGTT‑TGG‑GGA‑AATAAG‑C‑AGG‑CCCGCCCCTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACG‑GCACCAAAGATTTTTGAGTT‑ACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAAc < 1:498370/237‑1 (MQ=255) ca‑cga‑GCAT‑GGC‑‑GTTGAGGTGTGC‑GGA‑AATCAG‑A‑AAC‑CCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACG‑GCACCAAAGATTTTTGAGTT‑ACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGAc < 1:358178/239‑1 (MQ=255) cccca‑GCTT‑GGC‑GTTTAGGGT‑TGG‑GGG‑AATCGG‑A‑ATG‑CCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTTCATCGCTAAAGAAATGCCCACG‑GCACCAAAGATTTTTGAGTT‑ACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGAc < 1:672513/237‑1 (MQ=255) cccccgcgt‑GGC‑GGTTAGGGT‑GGG‑GAA‑AAGCAG‑C‑ACG‑CCCGCCACGTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAAGGCCCACG‑GCCCCAAAGATTTTTGAGTT‑ACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGAc < 2:247855/231‑1 (MQ=255) ccaacgtggggattAAGGT‑GGC‑GGG‑AACGAG‑C‑AAC‑CCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAATTACATCGCTAAAGAAATGCCCACG‑GCACCAAAGATTTTTGAGTT‑ACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCa < 1:526147/227‑1 (MQ=255) gcggggggTTAAAGT‑TGG‑GGGAAATCCG‑C‑AAC‑CCCGCCACTTTGACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACG‑GCACCAAAGATTTTTGAGTT‑ACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAg < 1:430526/233‑1 (MQ=255) t‑tGC‑GGG‑AAACAG‑C‑ACG‑CCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACG‑GCACCAAAGATTTTTGAGTT‑ACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGt < 1:471910/237‑1 (MQ=255) c‑GGG‑AATCAG‑C‑AAG‑CCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACG‑GCACCAAAGATTTTTGAGTT‑ACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGAta > 1:624129/1‑238 (MQ=255) c‑GGG‑AATCAG‑C‑AAG‑CCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACG‑GCACCAAAGATTTTTGAGTT‑ACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCCCGGCGCCCAAAACGAACTCTTCCAGCACCGGCCCCCCGATCATCTGAATTCCCCCTTCAACCACTGCCCGGCCAAAAATaaaactgccgtacatg > 2:861609/1‑226 (MQ=255) gaaataG‑C‑ATG‑CCCGCCAGTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACAGCGCTAAAGAAATGCCCACG‑GCACCAAAGATTTTTGAGTT‑ACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGaa < 1:861609/233‑1 (MQ=255) aaaacAG‑G‑AAG‑CCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACG‑GCACCAAAGATTTTTGAGTT‑ACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGaa < 1:182513/234‑1 (MQ=255) gaaccaagaaG‑CCCGACACTTTTGCCCGATTTTCCGGTCGGGCAGTGAAGTACATCGCTAAAGAAAGGCCCTCG‑GCACAAAAGATTCTTGAGTT‑ACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGaa < 1:835374/230‑1 (MQ=255) aaacgcagacg‑CCCGCCACGTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACG‑GCACCAAAGATTTTTGAGTT‑ACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGaa < 1:134641/228‑1 (MQ=255) aTCAG‑C‑AAG‑CCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACG‑GCACCAAAGATTTTTGAGTT‑ACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCCCAAACTGAACTCTTGCCGATGCTGGGCCCAGTACACCTGACTGCCGCCTTCAAACGCTGCCGGACCAAAGAGAAACTTGCCCTAGATGAAggg > 1:286019/1‑237 (MQ=255) attaacctaG‑CCCCCCCCTTTTACCCGATTTTCCGGTGCGGGAGTGAAGTACAGCGCTAAAGAAAGGCCCCCG‑GCACCAAAGATTTTTGAGTT‑ACCGGGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGAAg < 2:624129/230‑1 (MQ=255) g‑CCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACG‑GCACCAAAGATTTTTGAGTT‑ACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCACCAGCTGCCGGACCAAAGATAAAATGTCCGCAGATGAAGTGGTGTAAAc > 1:451896/1‑239 (MQ=255) | CGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCA‑CCA‑GCAT‑GGC‑GGTTAAGGT‑TGC‑GGG‑AATCAG‑C‑AAG‑CCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACG‑GCACCAAAGATTTTTGAGTT‑ACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGAAGTGGTGTAAAC > NC_000913/3862456‑3862914 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |