Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 421,608 T→G 25.1% V208G (GTG→GGG)  proY → proline‑specific permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913421,6080TG25.1% 87.5 / 2.3 56V208G (GTG→GGG) proYproline‑specific permease
Reads supporting (aligned to +/- strand):  ref base T (16/24);  new base G (12/4);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 3.66e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGTGTTCTACGTCGGTACGCTGTTCGTCATTATGTCTATCTACCCGTGGAATCAGGTTGGCACTGCCGGTAGCCCGTTCGTGCTGACGTTCCAGCATATGGGCATTACCTTTGCCGCCAGCATTCT  >  NC_000913/421382‑421842
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             
aTCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGAATTCATAACCTGTGGGGCAACGGCGGGTTCTTCAGTAACGGCTGGGCTTGCATGGGAATGGCGTTGCAAATGGGGAGGTTTGTTTa                                                                                                                                                                                                                                 >  1:594300/1‑239 (MQ=255)
             tAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGGAAGTTCGTTGCAAAAGGGGGTGTTTTGTTTCGGTtggggtgaa                                                                                                                                                                                                                    >  1:605175/1‑233 (MQ=255)
             tAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGGATTACTACACTGGGGGGCAAAGGGGGGTTCTTTCGGAACGGCCGGCTTGGCACGGTGAGTTTGTTCGAAATGGGGTGGTTTTGTTATGGTggggggtaa                                                                                                                                                                                                                    >  2:211700/1‑233 (MQ=255)
                     atgaGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCCTGGGATTGGAATGTCGTTTCACAAGGGGGAGTTTGGTTACCGTGGGATGGAAATTTTCgg                                                                                                                                                                                                            >  1:54504/1‑239 (MQ=255)
                          cGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCCTGGCCTGGGAATGTCGGTGACAATTGGGAGTTTTGCTTTCGGGGGGTTGGAAAATATTGGGAtt                                                                                                                                                                                                        >  1:859441/1‑238 (MQ=255)
                            tGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGTGCAACGGCGGCTTCTTCAGTACAGGCTGGCTTGGTCTGTGAATGTCGTTGCCAATGGGGGTGTTTTCTTTCGGTGGGGTCGGAATTTTTGGGGttta                                                                                                                                                                                                      >  1:586346/1‑236 (MQ=255)
                                    ttCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGGATTCATAAACTTTGGAGCAACGGCGGCTTCTTCCGGAACGGCTGGGTTTGCATGGGAAATGCGTTTCCAATGGGGCAGTTTGCTTTAGGGTGGGTCCAAAATATTCGGGTTTCCGCCggggg                                                                                                                                                                                             >  1:906628/1‑236 (MQ=255)
                                           aGCTGGAATTTTGGCTCTCGTTCTTTAAAGTCGCCGCCATCATCATCATGATGGTCGCCGGTTTCGGCAAAATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTGGGCATGGTAATGGCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGaa                                                                                                                                                                                       <  1:102913/238‑1 (MQ=255)
                                             cTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGAt                                                                                                                                                                                                                        <  1:150507/203‑1 (MQ=255)
                                                gAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGGTTGGCATGGTAAAGTCGTTGGAAATGGGGATGGTTGGTTACCGTGGGATCGGAATTATCGGGGTTACCGCCGGGGAAGCGAAAGAt                                                                                                                                                                                   >  1:603865/1‑237 (MQ=255)
                                                   ttgtGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTGGCTGGTTCCGGCATCATCTCCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTTTTCATAACCTGTGGAGCAACGGCGGCTTCTTAAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGGGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTga                                                                                                                                                                              <  1:645910/236‑1 (MQ=255)
                                                     ttgtttCTCTTTTTTTAAGGTCATCCCCAGCAATAGCATGATTGGCGCTGGTTTGGAATGCACAGTCGGGGGGATTGGCAAGGCGGGACACCCGTCGTGTATGCATAACCGGTGGGGCAACGGCGGCTTTTTCAGAAGGGCCGGGGTGGGATTGGTAAGGTCGTTGAAAATGGTGATGTTTGTTTCCGGTGGGATGAAAATGACCGGGATTACCGCCGGTGAAGCGAAAGATCCTgaga                                                                                                                                                                            <  2:435680/235‑1 (MQ=255)
                                                        gTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGGATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCCAATGGGGATGTTTTCTTACCGTGGGGTCGAAATTATTGGGATTACCGCCCGTGAAGCGAAAAATCCTTAGgaa                                                                                                                                                                          >  1:655466/1‑238 (MQ=255)
                                                              gTTCTTTAAAGTCGCCACCATCATCATCATGATTGCCGCCGGTTGCGGCATGATCATCTGGGGAATTGGCAACGGCGGGCAACCGACCGGTATTCATACCCTGGGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTGGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATAc                                                                                                                                                                   <  1:8329/239‑1 (MQ=255)
                                                                                    cccctcagtttttgccgcgtttgcGGCATCTTCGTCGGGGGGTTTGGCAACGGCGGGAACCCACCGGGTATTAATAACCTGTGGAGAAACGGCGGGTTTTTCATTAACGGCTGGTTTGGCATGGTAATGTCGTGGCAAATGGGGATGTTTGCTTCCGGTGGGATCGAAATTATCGGGTTTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGcc                                                                                                                                             <  2:476171/216‑1 (MQ=255)
                                                                                       atcatGTTTGTCGGCGTTTTCGGCATCAGCTTTGGGGGGATTGGCAAGGGGGGGCACCCGACCGGTTTCTATAACCTGTGGAGCAGCGGCGGTTTCATCATTACCGGTGGGCTGGGCTTGGTAATGTCGTTGCAAATGGTGATGTTTGCTTAGGGGGGGATGAAAATTATCGGGATTCCCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGAt                                                                                                                                          <  2:184163/239‑1 (MQ=255)
                                                                                       atcatGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGGGATGTTTGCTTACGGGGGGATCGAAATTTTCGGGATTACCGCCGGGGAAGCGCAAGATCCTGGGGAATCGATACCCGGGGCGCTTAACTCCGTGcccga                                                                                                                                          >  1:804387/1‑236 (MQ=255)
                                                                                       atcatGATTGCCGCCGGTTGCGGGATCATCATCGGGGGTATTGGCAACGGCGGGCAACCGACCGGTTTTCATAACCTGGGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGAt                                                                                                                                          <  1:327737/239‑1 (MQ=255)
                                                                                         catGATGGGCGCGGGTTCCGGCATCATCATCTGGGGGATGGGCAAGGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAGCGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGc                                                                                                                                        <  1:848277/239‑1 (MQ=255)
                                                                                                 gcccctgTTTCGGGATCGTCCTGTGGGGTATTGGCGACGGGGGCCAACCGACGTGTTTTCAAAACCTGGGGAGCACGGGCGGTTTCTTAGGTAACGGTTGGCTTGGCATGGTAATGTCGTTGAAAATGGTGATGTTTGCTTAGGGGGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTg                                                                                                                                <  2:777277/233‑1 (MQ=255)
                                                                                                 gcGCTGGTTTCGGCTTACTCCTTGGGGGGGTTGGGACGGGGGGGAACCCGGCCGGTTTTCATACCCTGGGGAGCACCGGCGGTTTCTTCAGTAAGGGCTGGCTTGGCAGGGAAATGTCGTTGAAAAGGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTg                                                                                                                                <  2:12231/238‑1 (MQ=255)
                                                                                                   gCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGtt                                                                                                                                                                                                                                        <  2:809582/134‑1 (MQ=255)
                                                                                                   gCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGtt                                                                                                                                                                                                                                        >  1:809582/1‑134 (MQ=255)
                                                                                                   gCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGGGATGTTTGCTTTCGGTGGGGTCGAAATTTTTGGGGTTAACGCCGGTGAAGGGAAAGATCCTGAGAAATCGATACCGCGGTCGATTAACTCCGGGCCGCTGGGGTTTCTGgt                                                                                                                              >  1:80710/1‑239 (MQ=255)
                                                                                                                    acatcTGGGGGTTGGGCAACGGCGGGCAACCGACGGTTTTTCATAACCTTGGGAGCAACGGGGGTTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGGGATGTTTGCTTACGGTGGGATCGAAATTATGGGTATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGTGTTCTACGTCGGTACg                                                                                                              <  1:230396/238‑1 (MQ=255)
                                                                                                                               tttGGAAACGGGGGCCCACCGACCGTTATTAATACCCTGGGGAGCACCGGGGGTTTCTTAATTACCGGGTGGTTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGGGGGATCGAATTTATCGGGTTTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGTGTTCTACGTCGGTACGCTGTTCGTCa                                                                                                    <  2:605175/237‑1 (MQ=255)
                                                                                                                                                acccgaccgTTTTTCATAACCTGTGGAGCAACGGCGGCTTCTTAAGTACCGGCTGGCTTGGCTTGGTAATTTCTTTGCAAATGGTGATGTTTGCTTACGGGGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGTGTTCTACGTCGGTACGCTGTTCGTCATTATGTCTATCTACCCg                                                                                  <  1:118263/237‑1 (MQ=255)
                                                                                                                                                    ccccgGTATCTATAACTGGTGGAGCAGCGGCTGTTTTTTCGTTAACGGTTGGTTTGGCATGGTAATGTCTTGTGAAAGGGTGAGTTTTGCTTCCGGGGGGATCAAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGTGTTCTACGTCGGTACGCTGTTCGTCATTATGTCTATCTACCCGTGGa                                                                              <  2:583891/237‑1 (MQ=255)
                                                                                                                                                             ttCATACCCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTga                                                                                                                                                                              <  1:794827/136‑1 (MQ=255)
                                                                                                                                                                 tccccTTTGGTGCAACGGCGGTTTCTTCAGTAACGGGTGGCTTGGCATGGTAATGTCGTTGAAAATGGTGATGTTTGCTTACGGTGGAATCGAAATTACCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGTGTTCTACGTCGGTACGCTGTTCGTCATTATGTCTATCTACCCGTGGAATCAGGTTGGCa                                                                  <  1:919526/236‑1 (MQ=255)
                                                                                                                                                                     cTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGGGTTCTGCGTCGGTACGCTGTTCGGCATTATGTCTTTCTACCCGTGGAATCCgg                                                                        >  1:604440/1‑229 (MQ=255)
                                                                                                                                                                       gtgGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTTGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATTGGGATTAACGCCGGTGAAGCGAAAGATTCTGAGAAATCGATAACGCTGGCGGTTAACTCCGGGCCGATGCGTATTCTGGGGTTTCACGTCGGGAACCGGTTCGTCATTTTGTCTATTTACCCCGGGGATCCGGTTggtccttcgg                                                            >  2:603035/1‑231 (MQ=255)
                                                                                                                                                                       gtgGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGGATTCTGGGGTTCTACGTCGGTACGCTGTTCGTCATTATGTCTATCTACCCGTGGAATCAGGTTGGCACTGCCg                                                            >  1:348902/1‑239 (MQ=255)
                                                                                                                                                                       gtgGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGGGGGATCGAAATTATCGGGGTTAACGCCGGGGAAGCGAAAGATCCTGGGAAATCGATa                                                                                                                                                                    >  2:794827/1‑136 (MQ=255)
                                                                                                                                                                       gtgGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGGGATGTTTGCTTACGGTGGGATCGAAATTATCGGGCTTACCGCCGGGGAAGCGAAAGGTCCTGAGAAATCGATACCGCGTGCGATTAACTCCCGGCCGTAGCGGCTTCTGGGGTTTTACGGCGGGACGGTGTTCTGCATTTTGTCCATCTACCCCGGGAGACAAGTTgggcatgccg                                                            >  2:547378/1‑231 (MQ=255)
                                                                                                                                                                        tgGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGGTGCAAATGGTGATGTTTTCTTACGGTGGGGTCGAAATTATCGGGGTTAACGCCGGGGGAGCGAAAAATCCGTGGAAATCGATACCGCGTGCGATTAACCCCGTGCCGATGCGGATTCTGGGTGTCTACGCAGGGACCCCTTCCGTCATTTTTCGCATTTACCCGTGGGATcgaggggggccggccgg                                                           >  2:156490/1‑223 (MQ=255)
                                                                                                                                                                          gtgCAACGGCGGCTTCTTCAGTAACGGCTGACATGGCATTGCGATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGGTTACCGCCGGTGAAGGCAAAGGTCCTGAGAAATCGTAACCGCGCGCGACTAACTCCATGCCGATGCGTATTTTGGTGTTCCTCCGCGGGACGGTTTTTGTCATTATTGTGTCTATCCCCTGGTCTCAGGTTTGCACGCCCggca                                                         >  2:602123/3‑237 (MQ=255)
                                                                                                                                                                             cAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGGTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGTGTTCTACGTCGGGACGCTGTTCTTCATTATGTCTATCTACCCGTGGAATCAGGTTGGCACTGCCGGTAGc                                                       >  1:903645/1‑238 (MQ=255)
                                                                                                                                                                              aaCGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAAACGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGGGTTCTTCGTCGGGACCCTGTTCGGCCTTTTTTCTATCTACCCGGGGGATAAGGTTGGCACTCCCGGGAGccc                                                     >  2:247627/1‑239 (MQ=255)
                                                                                                                                                                               aCGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTAt                                                                                                                                                                                                               <  1:216072/85‑1 (MQ=255)
                                                                                                                                                                               aCGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTAt                                                                                                                                                                                                               >  2:216072/1‑85 (MQ=255)
                                                                                                                                                                               aCGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGTGTTc                                                                                                                          >  1:8832/1‑170 (MQ=255)
                                                                                                                                                                               aCGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGTGTTc                                                                                                                          <  2:8832/170‑1 (MQ=255)
                                                                                                                                                                                     gcTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCTATACCCCGGGCGTTTAACCCCGTGCCGCTGCGTATTCTGGTGTTTTACGTCGGTCGGCTTTTCGTCATTATGGCTTTTTACCCGGGGAGTCAGGTGGGACATGCCGGTAGCCCCttggg                                               >  2:912251/1‑235 (MQ=255)
                                                                                                                                                                                                      tggttgggCGTGGTAATGTGGTTGAAAATGGTGATGTTTGCTTACGGGGGGATCGAATTTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGTGTTCTACGTCGGTACGCTGTTCGTCATTATGTCTATCTACCCGTGGAATCAGGTTGGCACTGCCGGTAGCCCGTTCGTGCTGACGTTCCAGCata                              <  1:466362/232‑1 (MQ=255)
                                                                                                                                                                                                      tggttTGGCATGATTATGTTGGTGAAAGTGGTGTTGTTTGGTTGGGGGGGGAGCAAATTTTGCGGGATTCCCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGTGTTCTACGTCGGTACGCTGTTCGTCATTATGTCTATCTACCCGTGGAATCAGGTTGGCACTGCCGGTAGCCCGTTCGTGCTGACGTTCCAGCata                              <  2:329256/238‑1 (MQ=255)
                                                                                                                                                                                                      tggttTGGAATGGAAAGCTCGGTGCAAGTGGTGTTGTTTGCTTACGGGGGGATCGAATTTTCCGGTATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGTGTTCTACGTCGGTACGCTGTTCGTCATTATGTCTATCTACCCGTGGAATCAGGTTGGCACTGCCGGTAGCCCGTTCGTGCTGACGTTCCAGCata                              <  1:601518/238‑1 (MQ=255)
                                                                                                                                                                                                      gtgctTGGCATGGGAATGGCGTTGCAAGTGGGAATGTTTGCTAACGGGGGGATCGAAATTACCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGTGTTCTACGTCGGTACGCTGTTCGTCATTATGTCTATCTACCCGTGGAATCAGGTTGGCACTGCCGGTAGCCCGTTCGTGCTGACGTTCCAGCata                              <  1:635227/236‑1 (MQ=255)
                                                                                                                                                                                                      gggctTGGCAGGGTAATGTCGTTGCAAAGGGGATTTGTTGATTACGGTGGGATGGAATTTAGCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGTGTTCTACGTCGGTACGCTGTTCGTCATTATGTCTATCTACCCGTGGAATCAGGTTGGCACTGCCGGTAGCCCGTTCGTGCTGACGTTCCAGCata                              <  1:553811/237‑1 (MQ=255)
                                                                                                                                                                                                        gctTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGTGTTCTACGTCGGTACGCTGTTCGTCATTATGTCTATCTACCCGTGGAATCAGGTTGGCACTGCCGGTATCCCGTTCGTGCTTCCGTTCCAGCATATg                            >  1:760658/1‑238 (MQ=255)
                                                                                                                                                                                                         ctTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGTGTTCTACGTCGGTACGCTGTTCGTCATTATGTCTATCTACCCGTGGAATCAGGTTGGCACTGCCGGTAGCCCGTTCGTGCTGACGTTCCAGCATATgg                           >  1:329256/1‑238 (MQ=255)
                                                                                                                                                                                                                           gTTGCAAATGGTGATTTTTGCTTACGGTGGGATCGAAATTAGCGGGATTCCCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGTGTTCTACGTCGGTACGCTGTTCGTCATTATGTCTATCTACCCGTGGAATCAGGTTGGCACTGCCGGTAGCCCGTTCGTGCTGACGTTCCAGCATATGGGCATTACCTTTGCCGCCa         <  1:211700/239‑1 (MQ=255)
                                                                                                                                                                                                                             tgaaaaGGGTGATGTTTGCTTACGGGTGGAACAAAATTAGCGGAATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGTGTTCTACGTCGGTACGCTGTTCGTCATTATGTCTATCTACCCGTGGAATCAGGTTGGCACTGCCGGTAGCCCGTTCGTGCTGACGTTCCAGCATATGGGCATTACCTTTGCCGCCAGc       <  1:547378/236‑1 (MQ=255)
                                                                                                                                                                                                                             tGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGTGTTCTACGTCGGTACGCTGTTCGTCATTATGTCTATCTACCCGTGGAATCAGGTTGGCACTGCCGGTAGCCCGTTCGTGCTGACGTTCCAGCATATGGGCATTACCTTTGCCGCCAGc       >  1:241199/1‑239 (MQ=255)
                                                                                                                                                                                                                              gCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTATGGTGTTCTACGTCGGTACGCTGTTCGCCCTTATGTCTATCTACCCGTGGAATCAGGTTGGCACTGCCGGTAGCCCGTTCGTGCTGACGTTCCACCATATGGGAATTACCTTTGCCCCCAGCa      >  2:670624/1‑239 (MQ=255)
                                                                                                                                                                                                                                  ttGGTGTGGTTTGTTTACGGTGGGATCGAATTTGCCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTACCGATGCGTATTCTGGTGTTCTACGTCGGTACGCTGTTCGTCATTATGTCTATCTACCCGTGGAATCAGGTTGGCACTGCCGGTAGCCCGTTCGTGCTGACGTTCCAGCATATGGGCATTACCTTTGCCGCCAGCATTCt  <  1:277805/238‑1 (MQ=255)
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ATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGTGTTCTACGTCGGTACGCTGTTCGTCATTATGTCTATCTACCCGTGGAATCAGGTTGGCACTGCCGGTAGCCCGTTCGTGCTGACGTTCCAGCATATGGGCATTACCTTTGCCGCCAGCATTCT  >  NC_000913/421382‑421842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: