BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,017,124	A→G	8.8%	E232G (GAG→GGG)	udp →	uridine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,017,124	0	A	G	8.8%	354.2 / 2.4	140	E232G (GAG→GGG)	udp	uridine phosphorylase
Reads supporting (aligned to +/- strand): ref base A (62/60); new base G (11/4); total (76/64)
Fisher's exact test for biased strand distribution p-value = 1.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 142 total aligned reads displayed.

GCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTGGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTA  >  NC_000913/4016890‑4017362

gggTGACAGGTTCTTCTGATTCCTTCTACCCAGGTCAGGAACGTTACGTTCCTTACTCTGGGCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTGGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACg                                                                                                                                                                                                                                               <  2:167345/237‑1 (MQ=255)

     aCAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTGGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGa                                                                                                                                                                                                                                           <  1:547364/238‑1 (MQ=255)

          ttcttcTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTGGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGCATGGTAGCGGGTGTTAT‑CGTTAACCGCCCCCGGCAAGGGA‑TCCCGATTGCTGAGACGTTGaaccaa                                                                                                                                                                                                                                      >  1:171325/1‑238 (MQ=255)

          ttcttcTGATACCGTCCACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTGGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGaaac                                                                                                                                                                                                                                        <  1:51216/236‑1 (MQ=255)

                       ttCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGGAGAGTGGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGGGCCGGTTTGGCAGCGGGGGCTAT‑GGTTAAACGCCCCCAACAA‑AGAGTCCCGAATGCTGGGCGGAGGAAAAAAAACAAAAACCaagg                                                                                                                                                                                                                        >  2:352401/1‑236 (MQ=255)

                       ttCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTGGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATTGGGGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTTT‑CGTTAACCGCACCCAGCCAGAGA‑TGCCGAATGCTGAGAGGATGAAAAAAACCCaacaccaagg                                                                                                                                                                                                                        >  2:715770/1‑231 (MQ=255)

                        tCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTGGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAAACCTGCTGACCATGTGTGCAAGTCAGGGCCTGAGTGCCGGTATGGTAGCGGGGGTTAT‑AGTTAACCGCACCCAGCCACAGA‑TCCCGGAGGCTGCGACGAGGAAACAAACCGAAAACCacggg                                                                                                                                                                                                                       >  2:306442/1‑235 (MQ=255)

                         cTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTGGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCCAATGCTGAGACGATGAAACAAACCGAAAGCCATgggg                                                                                                                                                                                                                      >  1:430308/1‑239 (MQ=255)

                            ccccGGGCAGGAAGGTTTCGAAACTTTCTCTGGTGGGGTAGTTCGTGACTTTAAAGGTTCTATGGAAGAGTGGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGa                                                                                                                                                                                                                   <  2:394854/239‑1 (MQ=255)

                                 gTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTGGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAATCCTGCTGACCATGGTTGCAAGTCAGGGCCTGCGGGCCGGTATGGTAGCGGGCGTTAC‑CGTTTACCGGACCCCGCAAGGTA‑TTCCGGAGGCTGCGAACAGGAAACAAACCGaaaacacaccgggggaaaac                                                                                                                                                                                                              >  2:424941/1‑222 (MQ=255)

                                           ttACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTGGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGGGACGATGAAACAAACCGAAAGCAATGCGGTGAAAATCGTGGGGGAAg                                                                                                                                                                                                    >  1:58639/1‑239 (MQ=255)

                                           ttACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTGGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCAAGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGAGGGTGTTAT‑CGTTAACCGGACCCAGCCAGAGT‑GCCCGAATGCTGAGGCGATGAAACAAACCTAAAGCCATGCGGGGAAAATCGCGGGGGAAg                                                                                                                                                                                                    >  1:65632/1‑239 (MQ=255)

                                                 tacttactCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTGGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCCCCCAGCAAGAGA‑TCCCGAATGCTGGGACGATGAAACAAACCGAAAGCCAGACGGGGAAAAACGGGGGGGAAacggcgg                                                                                                                                                                                              >  2:59589/1‑238 (MQ=255)

                                                       agctGGTTGTGTTGGTCGTCAATTTAAAGTTTCTATGGAAGGTTGGCCGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCGTGTGTGCAAGTCAGGGCCGGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCg‑tcgtc                                                                                                                                                                                       <  2:65632/237‑1 (MQ=255)

                                                           ggTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTGGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGGGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGCAAGCCATGCGGGGCAAATCGGGGTGGAAGCGGCGCG‑GCGTctcgt                                                                                                                                                                                   >  1:435372/1‑236 (MQ=255)

                                                           ggTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTGGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGGGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAACCATGCGGGGAAAAACGGGGGGGaaagggggggccggctggg                                                                                                                                                                                    >  2:120302/1‑222 (MQ=255)

                                                            gTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTGGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGGACCCCGAAAGGAA‑TCCCGAATGCGGAGACGATGAAAAAAACCCAAAACCATGCGGGGAAAACTGGGGTGGAAgggggggggcgttggcgt                                                                                                                                                                                   >  2:600525/1‑225 (MQ=255)

                                                             tCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTGGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑CCGTCGGCTGt                                                                                                                                                                                 >  1:731934/1‑239 (MQ=255)

                                                              cctttAGTTCGTCACTTTAAAGGTTCTAGGGAAGAGTGGCAGGGGATGGGAGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTa                                                                                                                                                                                <  2:748719/235‑1 (MQ=255)

                                                                cgTAGTTCGTCACTTTAAAGGTTCTAGGGAAGAGTGGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTaa                                                                                                                                                                               <  1:352401/238‑1 (MQ=255)

                                                                     ttCGTCACTTTAAAGGTTCTATGGAAGAGTGGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTaa                                                                                                                                                                               <  1:397136/233‑1 (MQ=255)

                                                                      tCGTCACTTTAAAGGTTCTATGGAAGAGTGGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGGGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGGGACGTTGAAAAAAACCGAAAGCCCTGCGGTGAAAATCCGGGGGGAAgcggggggtggccgacggtaaattttt                                                                                                                                                                          >  2:81026/1‑215 (MQ=255)

                                                                           catttAAAGGTTCTATGGAAGGGTGGCAGGCGATGGGCGGAATGAACTGTGAAATGGAATCTGCAACCCTGCTGGCCATGTGGGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCt                                                                                                                                                                  <  2:468156/237‑1 (MQ=255)

                                                                               tAAAGGTTCTATGGAAGAGTGGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTg                                                                                                                                                                 >  1:30818/1‑236 (MQ=255)

                                                                               tAAAGGTTCTATGGAAGAGTGGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCCAATGCTGAGACGATGAAAAAACCCGAAAGCCCAGCGGGGCAAATAGAGGGGGGAGGGGGGCG‑GCGCctgtgttaaatttcttaccctgctt                                                                                                                                                               >  2:700670/1‑217 (MQ=255)

                                                                                       cTATGGAAGAGTGGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGGTGAAACAAACCGAAAGCCATGCGGGGAAAATCGTGGGGGAAGCGGCGCG‑GCGGCTGCTGAAATTCTCT‑TCTCCTgctgacaggcgg                                                                                                                                                      >  2:879273/1‑228 (MQ=255)

                                                                                       cTATGGAAGAGTGGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTCCTGTAGTTCTCT‑TTTCCTGTCTGAAGGcgg                                                                                                                                                      >  1:477755/1‑237 (MQ=255)

                                                                                               gagTGGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGGGA‑TCCCGCATGCTGAGACGATGAAACAAACCGAAAGCCCTGCGGGGAAAAACGTGGGGGGAGCGGCCCG‑GCGTCTGCAGTAAATCTTT‑TCTCCCGTCTGAAGGCCgcgcggggg                                                                                                                                              >  2:445400/1‑231 (MQ=255)

                                                                                               gagTGGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGCAAGCCATGCGGTGAAAATCGTGGTGGGAGCGGTGCGCGCGCCTG‑TGTAATTCTCT‑TCCCCTGTCGGAAGAcccgggggggc                                                                                                                                              >  2:786168/1‑230 (MQ=255)

                                                                                               gagTGGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGGCGG‑TCGTCTGCTTGAATTTTCT‑TCTCCTGTCCGAAAGCCGACGCGttg                                                                                                                                              >  2:575832/1‑238 (MQ=255)

                                                                                                  tGGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGGGCAAGTCAGGGCCTGCGGGCCGGGATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGC‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGGGAAAATCGTGGGGGAAGCGGCCCG‑GCGGCGCTTGTAATT‑TCTCTCCCCTGTCTTAAGGCCGAcggggtggg                                                                                                                                            >  2:509504/1‑231 (MQ=255)

                                                                                                   ggCAGGCGATGGGCGTAATGAACTATGAAATGGAGTCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAAGCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCggac                                                                                                                                          <  1:424941/239‑3 (MQ=255)

                                                                                                    gCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGGCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGcc                                                                                                                                          <  1:912865/238‑1 (MQ=255)

                                                                                                       ggCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCAGGCTGAAGGCCGACGCCGTCGTCC‑tttt                                                                                                                                     >  1:830132/1‑239 (MQ=255)

                                                                                                            tGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGGGAAAATCGTGGTGGAAGCGGAGCG‑TCGTCTGCGGTAATTTTCT‑TCTCCTGTCTGAAGGCCGACGCGTCCGGCC‑TTTTGCAtt                                                                                                                                >  1:796377/1‑239 (MQ=255)

                                                                                                                    aTGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGGGGGAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TATCCTTTCTGATGGCCCACCGGTTCGGCC‑ttttttttttttcgtt                                                                                                                         >  2:91730/1‑234 (MQ=255)

                                                                                                                        aCTATGAAATGGGATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCCCCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAgcgc                                                                                                                    <  1:824506/239‑1 (MQ=255)

                                                                                                                            tGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATC‑TGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGGGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGc                                                                                                               >  1:472761/1‑239 (MQ=255)

                                                                                                                             gAAATTGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGc                                                                                                               <  1:231949/239‑1 (MQ=255)

                                                                                                                                 tGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGGAGCGGCGCG‑TCGTCTGCGGGACGTCTAT‑TCTCCTTCCTGAAGGCCGACGCGTTGGGCC‑TTTTTCATTTTTGCgagcagccctgcggga                                                                                                           >  2:91236/1‑224 (MQ=255)

                                                                                                                                 tGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGGGAAAATCGTGGGGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGCCC‑TTTTGTATTTTTGCGTAGCGCCTCGCGGGa                                                                                                           >  2:571847/1‑239 (MQ=255)

                                                                                                                                     aTCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAAt                                                                                                        <  1:91730/238‑1 (MQ=255)

                                                                                                                                           aaCCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGAACCCAGCAAGCGA‑TCCCGAATGCTGGGACGATGAAACAACCAGAAAGCCATGCGCTGAAAATCGTGGTGGAAGCGGGGCG‑CCGTCTGCTGTAGTTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGAC‑TTTGGTATTTTTGCGTAGcccccaccaggacacaacttc                                                                                                  >  2:112822/1‑221 (MQ=255)

                                                                                                                                              ccggctgACGTTGTGTGCAAGGCAGGGCCGGGGGGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCaa                                                                                              <  2:826233/236‑1 (MQ=255)

                                                                                                                                                 gggggccctttgtgGAAGGCGGGGCCTGGGGTCCGGTATGGGAGCGGGGGTTAC‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTg                                                                                           <  2:503550/229‑1 (MQ=255)

                                                                                                                                                 gctgCCCGTTTGTGCAAGTCGGGGCCTGCGTGCCGGTTTGGTAGCGGGTGTTTT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGGGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTTTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTg                                                                                           <  2:477755/239‑1 (MQ=255)

                                                                                                                                                 gctgACCATGTGGACAAGTTAGGGCCTGCGTGACGGTATGGTAGCGGGTGTTAT‑CGTTAGCCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTg                                                                                           <  1:905750/239‑1 (MQ=255)

                                                                                                                                                  ctgACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCATGTCTGAAGGCCACCGCGTTGGGCC‑TTTTGTATTTTTGCGTAGCGCCCCCCAGGAAATCCCTTTCCAATTgg                                                                                          >  2:781374/1‑239 (MQ=255)

                                                                                                                                                    gACCTTGGGTGGAAGTCGGGGCCTGCGGGCCGGTATGGTGGGGGGTGTTAA‑CGTTAACCGCCCCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGAc                                                                                        <  2:435372/239‑1 (MQ=255)

                                                                                                                                                     cccATGTGTGCAAGTCCGGGCCTGCGGGCCGGTATGGTAGCGGGGGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATC‑TGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGt                                                                                      <  2:472761/238‑1 (MQ=255)

                                                                                                                                                      ccctgtttgacagctaGGGTCTGGGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGt                                                                                      <  1:758660/224‑1 (MQ=255)

                                                                                                                                                       cATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGt                                                                                      <  1:678664/238‑1 (MQ=255)

                                                                                                                                                       cATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGGGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGAATTTTTTCTTAGGGCCTCCCAGGGAAGGCCTTTCCAACTggggggt                                                                                     >  2:447949/1‑234 (MQ=255)

                                                                                                                                                         tgtgtgCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCGGTATTTCTCT‑TCTCCTGTCTGGAGGCCTACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGGTCCTCGACGGAAAAGCCTTTCCAACTGGCCGttgg                                                                                   >  1:556707/1‑237 (MQ=255)

                                                                                                                                                          gtgtgCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAAt                                                                                                        >  2:480822/1‑217 (MQ=255)

                                                                                                                                                          gtgtgCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAAt                                                                                                        <  1:480822/217‑1 (MQ=255)

                                                                                                                                                           tgtgCAAGGCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTa                                                                                 <  1:509504/239‑1 (MQ=255)

                                                                                                                                                            gtgCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGCAATTATCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGGATTTTTGCGTCGCGCCCCGCAGGAAATGCCTTTCCACCTGGACGtttttca                                                                                >  1:283341/1‑235 (MQ=255)

                                                                                                                                                               cAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGc                                                                             >  2:184901/1‑239 (MQ=255)

                                                                                                                                                               cAAGGCAGGGCCTGCGGGCCGGTTTGGTCGCGGGTGTTTT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGGGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGc                                                                             <  2:681867/239‑1 (MQ=255)

                                                                                                                                                                aagcaggggccgtggtccgtgtttGGTAGCGGG‑GTTATGCGGTAACCCCACCCAGTGAGAGA‑ACCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGca                                                                            <  2:745436/216‑1 (MQ=255)

                                                                                                                                                                aaGGCAGGGCCTGCGGGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGca                                                                            <  1:756753/239‑1 (MQ=255)

                                                                                                                                                                 aGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGca                                                                            <  2:30818/238‑1 (MQ=255)

                                                                                                                                                                 aGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGca                                                                            <  1:445400/238‑1 (MQ=255)

                                                                                                                                                                 aGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGca                                                                            <  1:871947/238‑1 (MQ=255)

                                                                                                                                                                     aGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCTTAGCGCCTCGCAGGAAATGCCTTTCCCAATGGACGTTTGTAcctttctttt                                                                       >  2:599358/1‑230 (MQ=255)

                                                                                                                                                                     aGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTCAACCGCACCGCGCAAGAGA‑TCCCGAATGCTGAGCCGATGAAACAAACCGATAGCCATGCGGTGGAAATCATGGTGGAAGCGGCGGG‑TCGTCTACAGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACCCACTCGGCCATTTTGTTTTTTTGTAT‑GCCCCTCCCAGGAAATTCCTTTCCCACTGAACGttttatcccgccaac                                                                        >  2:796543/1‑226 (MQ=255)

                                                                                                                                                                       ggCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGAAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTTTATTTTTGCGTAGCGCCTCCCAGGAAATGCCTTTCCAACTGGACGTTTGTCTAGCCCAATTCt                                                                     >  1:404711/1‑239 (MQ=255)

                                                                                                                                                                            gCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTGGGCC‑TTTTGTATTTTTGCGTAGGCCCGTGCGGGAAATGCCTTTCCACATGGGCGTTTGTAAAGCACAATTCATtttt                                                                >  2:25744/1‑239 (MQ=255)

                                                                                                                                                                            gCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTa                                                                                 <  1:910762/222‑1 (MQ=255)

                                                                                                                                                                             cGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGCCGTTTGTACAGCACAATTCTTTTTtg                                                               >  2:565668/1‑239 (MQ=255)

                                                                                                                                                                             cGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCAAttttt                                                               >  1:713455/1‑238 (MQ=255)

                                                                                                                                                                                gCCGGTTTGTTTGGGGGTTTTTT‑CGTTACCCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGGCGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTtgtg                                                             <  2:50733/238‑1 (MQ=255)

                                                                                                                                                                                 ccGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGACAGAGA‑TCCCGAATGCTGGGACGATGCAACAAACCGAAAGCCATGCGGTGAAACTCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TTTCCTGTCTGCAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGGAGAGCCTCGAAGGAAATGCCTTTCCAACTGGCCGTTTGTCCAGGAAAATTCAAtttttg                                                              >  2:227322/1‑234 (MQ=255)

                                                                                                                                                                                    ggaTGGTGGCGGGTTTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCGGAGACCGTGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCgg                                                          <  2:561416/235‑1 (MQ=255)

                                                                                                                                                                                              gggTTTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGttgttg                                               <  1:120302/238‑1 (MQ=255)

                                                                                                                                                                                              gggTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGc                                              >  1:561416/1‑239 (MQ=255)

                                                                                                                                                                                               ggTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTTTGCGGGTAAGTTGTTGCg                                             >  1:520180/1‑239 (MQ=255)

                                                                                                                                                                                                 tgtTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAATCAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTg                                                                                           <  1:583913/191‑1 (MQ=255)

                                                                                                                                                                                                 gttttagtgTTAACCGCCCCCAGTAAGAGA‑TCAGGAATGCTGAGACGATGAAACAAACCGAAATCCATGCGGTGAAAATCGTGGTGGAAGCGGCGGG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCg                                             <  2:796377/230‑1 (MQ=255)

                                                                                                                                                                                                       acGTTAACCGGCCCCGGCAAGAGA‑TCCAGAATGCTGGGCCGATGAAACAAACCGAAGGCCTTGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCaggag                                      <  1:447949/238‑1 (MQ=255)

                                                                                                                                                                                                          ttAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCg                                   <  1:81026/239‑1 (MQ=255)

                                                                                                                                                                                                           tAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGCAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGt                                  >  1:550180/1‑239 (MQ=255)

                                                                                                                                                                                                            aaCCGCACCCAGCAAGAGA‑TCCCGAATGCTGCGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGGGtt                                 >  1:866915/1‑239 (MQ=255)

                                                                                                                                                                                                                gCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTgg                             <  1:715770/239‑1 (MQ=255)

                                                                                                                                                                                                                 cACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCGGGAGGGGTTGTGGa                            >  1:113647/1‑239 (MQ=255)

                                                                                                                                                                                                                  aCCCATCAAGAGA‑TCGCGAATGCTGAGACGATGAAACAATCCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTGTGCGTAGCGCCTCGCAGGAAATGCTTTTCCAACTGGACGTTTGTACAGCACAATTCTAtt                                                                  <  1:131730/200‑1 (MQ=255)

                                                                                                                                                                                                                  aCCCAGTAAGAGA‑TCCCGAAGTCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGGCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGa                            <  1:700670/238‑1 (MQ=255)

                                                                                                                                                                                                                        cAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAATCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACCCGTTCGCCC‑TTTTGTATTTTTGCGTAGCGCTTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCTGCCGTGGGCGTTGTGGATTTCTCa                     >  1:606151/1‑239 (MQ=255)

                                                                                                                                                                                                                            gggctCCCTAATGCTTGTACGATGAATGAAACCTAAAGCCATGCGGTGAAAAGCGTGGTGGAAGGGGTGGG‑TGTCCTTCTGTAATTCTCT‑TCTCCGGTCTGAAGGCAGACGCGTTCGGCC‑TTTTGTATTTGTGCGTAGCGCCTCGCAGGAAATGCTTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATc                  <  2:450742/235‑1 (MQ=255)

                                                                                                                                                                                                                              a‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCg‑tcgtc                                                                                                                                                                                       <  2:635005/73‑1 (MQ=255)

                                                                                                                                                                                                                              a‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCg‑tcgtc                                                                                                                                                                                       >  1:635005/1‑73 (MQ=255)

                                                                                                                                                                                                                                 cccGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCCCAATTCTATTTTGTGCGGGTAAgttg                                                  >  2:157527/1‑202 (MQ=255)

                                                                                                                                                                                                                                 cccGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCCCAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGCTTTCTCATTCATGGt             >  1:697/1‑239 (MQ=255)

                                                                                                                                                                                                                                 cccGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAgttg                                                  <  1:157527/202‑1 (MQ=255)

                                                                                                                                                                                                                                    gAATGCTGAGACGAGGAAACCACCCGAAGCCCATGCGGTGAAAATCGTGGTGGAAGCGGCGGT‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TGTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTa          <  1:919355/239‑1 (MQ=255)

                                                                                                                                                                                                                                      aTGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACg        <  1:600525/239‑1 (MQ=255)

                                                                                                                                                                                                                                      aTGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACg        <  1:184901/239‑1 (MQ=255)

                                                                                                                                                                                                                                      aTGCTGAAGCGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCGCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACg        <  1:790362/239‑1 (MQ=255)

                                                                                                                                                                                                                                         cTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTACGGCCTCGCAGGAAATGCCTTTCCAACTGGCCGTTTTTACAGCACACTTCTATTTTGTCCGGGTAAGTTTTTGCGTTCGGTGGGGTTGTGGATTTCTCAACAATGGTTTCCGCAg     >  2:436976/1‑239 (MQ=255)

GCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTGGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTAT‑CGTTAACCGCACCCAGCAAGAGA‑TCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCG‑TCGTCTGCTGTAATTCTCT‑TCTCCTGTCTGAAGGCCGACGCGTTCGGCC‑TTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTA  >  NC_000913/4016890‑4017362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`