Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,708,844	C→G	9.7%	A642A (GCC→GCG)	rsxC →	SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,708,844	0	C	G	9.7%	121.2 / 4.0	46	A642A (GCC→GCG)	rsxC	SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base C (23/18);  new base G (3/2);  total (26/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

AACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGC‑GCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGAT‑CCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAA  >  NC_000913/1708565‑1709122                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                        aaCCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCGAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGC‑GCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCa                                                                                                                                                                                                                                                                                   <  2:2970/286‑1 (MQ=255)              ccACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGC‑GCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAACCAGCACAACAGGCCGAt                                                                                                                                                                                                                                                                    >  2:92811/1‑288 (MQ=255)                 cGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGC‑GCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGACCCAGAACAAAAGGTCGATCcgc                                                                                                                                                                                                                                                                >  2:750490/1‑289 (MQ=255)                 cGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGC‑GCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAACCGGAACAGCAAAAGGCTAATGCCGAGCCAGAACAACAGGTCCAACcg                                                                                                                                                                                                                                                                 >  2:390635/1‑288 (MQ=255)                                     ggcTATCGCCCGCGCCAAAGAGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACACCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGC‑GCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAg                                                                                                                                                                                                                                              <  2:405699/286‑1 (MQ=255)                                     agcTATCGCCCGCGCCAAAGCGCCCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAACCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGC‑GCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAg                                                                                                                                                                                                                                              <  2:608672/287‑1 (MQ=255)                                      gcTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGC‑GCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCTCCAAAGCACACAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACCACAGGTCGTTCCGCGCAAAGCAGCCGTCGACGcc                                                                                                                                                                                                                                            >  2:311731/1‑288 (MQ=255)                                              gcgcgCCAAAGCGGCCAAGCGTGAACAGCAACAGGCGAATGCGGAACCAGAACACCAGGTCGAGCCGCGCAAAACCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGC‑GCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGc                                                                                                                                                                                                                                    <  2:132779/287‑1 (MQ=255)                                              ccGAGCCAAAGCGGGCAAGCTGAAACAGCAACAGGCGAATACGGAACCAGAACACCAGGTGGAGCCACGCAAAACCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGC‑GCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGc                                                                                                                                                                                                                                    <  2:731481/288‑1 (MQ=14)                                                 cgcCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGC‑GCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGTAAGCTGGAACAGCCACAGGCTAATGCCGAGCCAGAACCACAGGGCGATCCGCTCAAAGCTGCCGTTGAAGCCGCTTGTGcccta                                                                                                                                                                                                                                >  2:654874/1‑287 (MQ=14)                                                 cgcCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGC‑CCAACGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCCACCGGCTAATGCCGGACCAGAACACCAGGTCTGTCCGCGCAACGCAGCGCTCGAACGCGCTTTTGACCg                                                                                                                                                                                                                                 >  1:465881/1‑288 (MQ=14)                                                                          cAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTTGATCCGC‑GCAAAGCCGCCGTCGAAGCAGCTATTGCCGGCGCCAAAGCACGCAAGCCGGAACAGCAACAGGCTAATGCCGAGCCAGAACAAACGTCCGACCCCCCCAACGAAGCCGTCGAAGCAGCAATGGACCGGACCAAAGCCACCAAACCGTAACAGc                                                                                                                                                                                                       >  1:212922/1‑289 (MQ=14)                                                                                     tGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGC‑GCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCCAAt                                                                                                                                                                                            >  2:625349/1‑289 (MQ=255)                                                                                          aaCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGC‑GCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCGGCCGTCGAAGCCGCTATCGCCCGAGCCAAAGCGCGCAAAAGCGAACCGCAACAGGCTAATGCGGa                                                                                                                                                                                       >  2:761197/1‑289 (MQ=255)                                                                                                   cccttgtggATCCACGTAAAACGGCCGTTCAAACAGCTATCGCCCGCGCCAAAGCGCTCAATCTGGAACATCAACAGGCTAATGCGAAACCAGAACAACAGGTCGATCCGC‑GCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAATCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCagaaga                                                                                                                                                                              <  2:269544/281‑1 (MQ=11)                                                                                                             tCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGC‑GCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCCAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAAGAGGTTGATCCGCGTAAAGCTGCGGTCCCAGCCGTTATTGCCCGCGCCAAAGCCAGCAAGCTGGTACAGCAACCAGGTaaagggcgaacacaaggccaggcgcac                                                                                                                                                                    >  1:192735/1‑264 (MQ=14)                                                                                                                              tCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGC‑GCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCg                                                                                                                                                    >  2:686168/1‑288 (MQ=255)                                                                                                                                  ggaggcccagacagcggCCAAAGGGGGAAACCTGGAAAAGAAACAGGCAAATGAGGACCCAGAAGCCCAGGTCGAACCGG‑CGAAAGCGGCCGTAGAGGCAGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAAGGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGa                                                                                                                                                <  1:677248/272‑1 (MQ=14)                                                                                                                                        tATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGC‑GCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGc                                                                                                                                                                                          >  1:295884/1‑240 (MQ=255)                                                                                                                                        tATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGC‑GCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGc                                                                                                                                                                                          <  2:295884/240‑1 (MQ=255)                                                                                                                                                                          cAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGC‑GCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCCGGAACAGCAACAGGCTAATGCCGAGCCAGAAAAACAGGTCGATCCGCGCAAAGCCGCCGGCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGTACAGCCACAGGCCAATGCGGAGCCAGAAGAACAGGTTGATCTGGTCAATGGTGCTGTACGCGCGACTATTGCACGCGCCAACGCatagaatgtcgggacgc                                                                                                       >  1:382098/1‑273 (MQ=14)                                                                                                                                                                            aCAGGCTAATGCGGAACCAGAAGAACAGGTCGAGCCGC‑GCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCaa                                                                                                     <  2:465881/289‑1 (MQ=255)                                                                                                                                                                            aCAAGCTAATGCGGAACCAGAAGAACAGCTCGATCCGC‑GCAAAGCCGGCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCaa                                                                                                     <  2:212922/289‑1 (MQ=255)                                                                                                                                                                                          aaCCAGAAGAACAGGTCGATCCGC‑GCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCCATTGCACGCGCCAAAGCCCGCAAGCCGGAACAGCAACAGGCTAATGCGGt                                                                                       >  2:100104/1‑289 (MQ=255)                                                                                                                                                                                          aaCAAGAAGAACAGGTAGATCCGC‑GCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGt                                                                                       <  2:622359/289‑1 (MQ=255)                                                                                                                                                                                           aCCAGAAGAACAGGTCGATCCGC‑GCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCAGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGt                                                                                       <  2:147507/288‑1 (MQ=255)                                                                                                                                                                                                aagaaCAGGTCGATCCGC‑GCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGACGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGACCAGGTTGATCCGCGCAAAGCTGCCGTCGACGCGGCTATTGCCCGCGCCAAAGCACGCAACCGGGAACAACAACCGGCTCATGCCGCCCCaga                                                                                 >  1:306617/1‑289 (MQ=14)                                                                                                                                                                                                aagaaCAGGTCGATCCGC‑GCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAAAAGGCTAATGCGGTACCaga                                                                                 >  2:86018/1‑289 (MQ=255)                                                                                                                                                                                                 agaaCAGGTCGA‑CCGCAGAAAAGCTGCCGCCGAAGCCGCTATGGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACACCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCa                                                                                   <  2:169887/286‑1 (MQ=255)                                                                                                                                                                                                        gTCGATCCGC‑GCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCCCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCCGCCGTTGACGCGGCTATTCCCCGCGCCCAACGCCGAAAGCCGGATCAGCAACCGGCTaccgacgcagcaaaagaaacga                                                                         >  1:726215/1‑268 (MQ=14)                                                                                                                                                                                                          cGATCCGC‑GCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCCCGCAAACCGGAACAGCAACCGGCTAATGCGGCGCCAGCAGAACCGGTTGATCCGCGCAAAGCGGCCGTTGACGCGGCTATTGCCCGCGCCCACGCACGCAAGATGGAACAGCAACAGGccgaaaccgctgccgaaggaacagt                                                                        >  1:3493/1‑264 (MQ=14)                                                                                                                                                                                                          cGATCCGC‑GCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCa                                                                                                      >  1:651991/1‑258 (MQ=255)                                                                                                                                                                                                          cGATCCGC‑GCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCa                                                                                                      <  2:651991/258‑1 (MQ=255)                                                                                                                                                                                                                gc‑gcAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCCCGCGCCAAAGCACGCAACCCGGAACAGCCACAGGCCAATGCGGTACCAGAAGAACAGGTTGAT‑cc                                                                 >  2:163099/1‑288 (MQ=255)                                                                                                                                                                                                                                     ctgtgctgtccagtgcgacagactgCAATCGGGAACAGCAAAAGGCGAATGCGGTACCAGAACAACAGTTCGATCCGCGCAAAGCCGCCATCGAGGCCGCTATTTCCCGAGCCAAAGCGCGCAAGCGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTCGATCCGCGAAAAGCTGCCGTCGAAGCGGCTATTGCGCGAGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGAT‑CCGCGCAAAGCGGCAGTTGCcg                                             <  1:625349/264‑1 (MQ=14)                                                                                                                                                                                                                                     ccatatggcgccGTGCGAAAGCACGGAAGTTGGAACAGGAACCGGCTAATGCCGAGCCAGTACAACAGGTCGATCCGAGCAAAGCCGCGGTCGTAGCCGCTATTGCCCGGGGCAAGGGGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGAT‑CCGCGCAAAGCGGCAGTTGCcg                                             <  1:761197/278‑1 (MQ=17)                                                                                                                                                                                                                                     ccGCCATTGCGCGTGACAAAGCGCGCACGCTGTAACAGCAACAGGCTAAGGCGGAGCAGGAACAACAGGTCGATCCGCGGAAAGCCGCCGTCGAAGCCGCGATTGCCCGAGGCAAAGCGCGCAAGCGGGAACAGCAACCGGCTAATGCGGAGCCAGAACAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGAT‑CCGCGCAAAGCGGCAGTTGCcg                                             <  1:163099/288‑1 (MQ=17)                                                                                                                                                                                                                                         tATTGCCAGTGCAAAAGCAGCCAACAGGGAACAGCAACCGGCTAATTCCGGGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGAT‑CCGCGCAAAGCGGCAGTTGCCGCGGCt                                        <  2:575796/289‑1 (MQ=18)                                                                                                                                                                                                                                                  tGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCCGGAACAGCAAAAGGCTAAAGCGGTACCCGAAAAACAGGTTGAT‑CCGCCACAAGCGGCACTTGCCGCGGGTCTTGCCcg                                >  1:508590/1‑288 (MQ=17)                                                                                                                                                                                                                                                  tGCCAAAGCACGCAAGCTGGAACAGTAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCACAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAAAAGAACAGGTTGAA‑TCGACCAAAGCGGCAGTTGCAGCGGACATTGCCcg                                >  1:9052/1‑288 (MQ=17)                                                                                                                                                                                                                                                   gCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGAT‑CCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCcgc                               >  2:439214/1‑288 (MQ=255)                                                                                                                                                                                                                                                     cAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGAT‑CCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCt                            <  2:306617/289‑1 (MQ=255)                                                                                                                                                                                                                                                                    ggAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGAT‑CCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAgcc             >  2:340127/1‑289 (MQ=255)                                                                                                                                                                                                                                                                     gAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGAT‑CCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAgcc             >  2:106295/1‑288 (MQ=255)                                                                                                                                                                                                                                                                           cAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGACGCTATGGCCCGCGCCAAAGCCCGCAAACGGGCACAGCAACCGGCTCATGGCGGGCAAGACGCACCAGATGATCCCGCCCAAGGC‑GCAGGTCCCGCTGCCATTGCCCGCGCCCaacgccgcaagacgtgacag       >  1:562337/1‑269 (MQ=14)                                                                                                                                                                                                                                                                               aGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGAT‑CCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGaa  <  2:3493/289‑1 (MQ=255)                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                        AACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGC‑GCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGAT‑CCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAA  >  NC_000913/1708565‑1709122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑