Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,233,947 C→T 9.1% intergenic (+110/‑84) yeiS → / → preT DUF2542 family protein/dihydropyrimidine dehydrogenase, NADH‑dependent, subunit N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,233,9470CT 9.1% 135.5 / 5.0 44intergenic (+110/‑84)yeiS/preTDUF2542 family protein/dihydropyrimidine dehydrogenase, NADH‑dependent, subunit N
Reads supporting (aligned to +/- strand):  ref base C (22/18);  new base T (2/2);  total (24/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

AAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGGTGTATGTCTGGCGAGCAAAAAATCCCGGACTCTTTTTCGCTTATATGGTGGCATATATCGGCTTCGGAATTTTATCTATCGGCATGATTGTTTATCTTATTTTCTATCGTTAATATTCCCCTCTCCAGTTAATTATTGAGAATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAA  >  NC_000913/2233671‑2234227
                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                        
aaGGCTGGCGCGCAGGGTCGATTGATAAACGGGTTAAAAATGCACCGGAACCTGTGTATGTCTGGCGAGCAAAAAATCCCGGACTCTTTTTCGCTTATATGGTGGCATATATCGGCTTCGGAATTTTATCTATCGGCATGATTGTTTATCTTATTTTCTATCGTTAATATTCCCCTCTCCAGTTAATTATTGAGAATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCtt                                                                                                                                                                                                                                                                              <  1:553513/289‑1 (MQ=255)
aaGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGGTGTATGTCTGGCGAGCAAAAAATCCCGGACTCTTTTTCGCTTATATGGTGGCATATATCGGCTTCGGAATTTTATCTATCGGCATGATTGTTTATCTTATTTTCTATCGTTAATATTCCCCTCTCCAGTTAATTATTGAGAATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCt                                                                                                                                                                                                                                                                               <  1:378565/288‑1 (MQ=255)
    cTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGGTGTATGTCTGGCGAGCAAAAAATCCCGGACTCTTTTTCGCTTATATGGTGGCATATATCGGCTTCGGAATTTTATCTATCGGCATGATTGTTTATCTTATTTTCTATCGTTAATATTCCCCTCTCCAGTTAATTATTGAGAATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTa                                                                                                                                                                                                                                                                             >  1:19805/1‑286 (MQ=255)
    cTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGGTGTATGTCTGGCGAGCAAAAAATCCCGGACTCTTTTTCGCTTATATGGTGGCATATATCGGCTTCGGAATTTTATCTATCGGCATGATTGTTTATCTTATTTTCTATCGTTAATATTCCCCTCTCCAGTTAATTATTGAGAATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTa                                                                                                                                                                                                                                                                             <  2:19805/286‑1 (MQ=255)
                cgcgATTGATAAACGGGTTAAAAATGCACCGGAACCGGTGTATGTCTGGCGAGCAAAAAATCCCGGACTCTTTTTCGCTTATATGGTGGCATATATCGGCTTCGGAATTTTATCTATCGGCATGATTGTTTATCTTATTTTCTATCGTTAATATTCCCCTCTCCAGTTAATTATTGAGAATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAg                                                                                                                                                                                                                                                                <  2:506509/287‑1 (MQ=255)
                 ggaaTTGATAAACGGGTTAAAAATGCACCGGAACCGGTGTATGTCTGGCGAGCAAAAAATCCCGGACTCTTTTTCGCTTATATGGTGGCATATATCGGCTTCGGAATTATATCTATCGGCGTGATTGTTTATCTTATTTTCTATCGTTAATATTCCCCTCTCCAGTTAATTATTGAGAATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTa                                                                                                                                                                                                                                                              <  2:302937/285‑1 (MQ=255)
                                          cACCGGAACCGGTGTATGTCTGGCGAGCAAAAAATCCCGGACTCTTTTTCGCTTATATGGTGGCATATATCGGCTTCGGAATTTTATCTATCGGCATGATTGTTTATCTTATTTTCTATCGTTAATATTCCCCTCTCCAGTTAATTATTGAGAATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCt                                                                                                                                                                                                                                    >  1:637401/1‑289 (MQ=255)
                                                    ggTGTATGTCTGGCGAGCAAAAAATCCCGGACTCTTTTTCGCTTATATGGTGGCATATATCGGCTTCGGAATTTTATCTATCGGCATGATTGTTTATCTTATTTTCTATCGTTAATATTCCCCTCTCCAGTTAATTATTGAGAATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCta                                                                                                                                                                                                                          <  2:713308/289‑1 (MQ=255)
                                                             cTGGCGAGCAAAAAATCCCGGACTCTTTTTCGCTTATATAGTGGCATATATCGGCTTCGGAATTTTATCTATCGGCATGATTGTTTATCTTATTTTCTATCGTTAATATTCCCCTCTCCAGTTAATTATTGAGAATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTa                                                                                                                                                                                                                  <  2:531370/288‑1 (MQ=255)
                                                                    gCAAAAAATCCCGGACTCTTTTTCGCTTATATGGTGGCATATATCGGCTTCGGAATTTTATCTATCGGCATGATTGTTTATCTTATTTTCTATCGTTAATATTCCCCTCTCCAGTTAATTATTGAGAATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACa                                                                                                                                                                                                                                                            >  1:274809/1‑239 (MQ=255)
                                                                    gCAAAAAATCCCGGACTCTTTTTCGCTTATATGGTGGCATATATCGGCTTCGGAATTTTATCTATCGGCATGATTGTTTATCTTATTTTCTATCGTTAATATTCCCCTCTCCAGTTAATTATTGAGAATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACa                                                                                                                                                                                                                                                            <  2:274809/239‑1 (MQ=255)
                                                                                                                cGGCTTCGGAATTTTATCTATCGGCATGATTGTTTATCTTATTTTCTATCGTTAATATTCCCCTCTCCAGTTAATTATTGAGAATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCttt                                                                                                                                                              >  2:771629/1‑289 (MQ=255)
                                                                                                                            tttATCTATCGGCATGATTGTTTATCTTATTTTCTATCGTTAATATTCCCCTCTCCAGTTAATTATTGAGAATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACt                                                                                                                                                  <  2:637401/289‑1 (MQ=255)
                                                                                                                                                tttATCTTATTTTCTATCGTTAATATTCCCCTCTCCAGTTAATTATTGAGAATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTTCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCtctc                                                                                                                              >  1:338940/1‑289 (MQ=255)
                                                                                                                                                  tATCTTATTTTCTATCGTTAATATTCCCCTCTCCAGTTAATTATTGAGAATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCg                                                                                                                             >  1:626067/1‑288 (MQ=255)
                                                                                                                                                  tATCTTATTTTCTATCGTTAATATTCCCCTCTCCAGTTAATTATTGAGAATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGc                                                                                                                            <  1:218630/289‑1 (MQ=255)
                                                                                                                                                   aTCTTATTTTCTATCGTTAATATTCCCCTCTCCAGTTAATTATTGAGAATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTTCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCt                                                                                                                           <  2:338940/289‑1 (MQ=255)
                                                                                                                                                        aTTTTCTATCGTTAATATTCCCCTCTCCAGTTAATTATTGAGAATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCttt                                                                                                                                                              >  1:162259/1‑249 (MQ=255)
                                                                                                                                                        aTTTTCTATCGTTAATATTCCCCTCTCCAGTTAATTATTGAGAATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCttt                                                                                                                                                              <  2:162259/249‑1 (MQ=255)
                                                                                                                                                        aTTTTCTATCGTTAATATTCCCCTCTCCAGTTAATTATTGAGAATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGCTTAAAGAAGCCTCTCGCTGTtta                                                                                                                      >  1:55960/1‑289 (MQ=255)
                                                                                                                                                          tttCTATCGTTAATATTCCCCTCTCCAGTTAATTATTGAGAATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCg                                                                                                                             <  1:512295/280‑1 (MQ=255)
                                                                                                                                                          tttCTATCGTTAATATTCCCCTCTCCAGTTAATTATTGAGAATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCg                                                                                                                             >  2:512295/1‑280 (MQ=255)
                                                                                                                                                              tATCGTTAATATTCCCCTCTCCAGTTAATTATTGAGAATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTtta                                                                                                                      >  1:505473/1‑283 (MQ=255)
                                                                                                                                                              tATCGTTAATATTCCCCTCTCCAGTTAATTATTGAGAATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTtta                                                                                                                      <  2:505473/283‑1 (MQ=255)
                                                                                                                                                                                         attattGAGAATAATTATTACTTCACCTGATAAGCTTCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGc                                                                                     <  2:696697/289‑1 (MQ=255)
                                                                                                                                                                                             ttGAGAATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATTTCACGACCCCCCCTGTAGTCAGGCTTGCCCg                                                                                  >  1:111323/1‑288 (MQ=255)
                                                                                                                                                                                                agaATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAactcact                                                                                                                                                                      >  2:283043/1‑201 (MQ=255)
                                                                                                                                                                                                agaATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAactcact                                                                                                                                                                      <  1:283043/201‑1 (MQ=255)
                                                                                                                                                                                                agaATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGcc                                                                                    >  1:655311/1‑283 (MQ=255)
                                                                                                                                                                                                agaATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGcc                                                                                    <  2:655311/283‑1 (MQ=255)
                                                                                                                                                                                                                  ccTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGc                                                                                         <  2:768669/260‑1 (MQ=255)
                                                                                                                                                                                                                  ccTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGc                                                                                         >  1:768669/1‑260 (MQ=255)
                                                                                                                                                                                                                  ccTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAAt                                                            >  1:558175/1‑289 (MQ=255)
                                                                                                                                                                                                                  ccTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAAt                                                            >  2:359086/1‑289 (MQ=255)
                                                                                                                                                                                                                             gCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCa                                                 >  1:502097/1‑289 (MQ=255)
                                                                                                                                                                                                                              cGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCaa                                                >  2:229840/1‑289 (MQ=255)
                                                                                                                                                                                                                                     cacaTTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACtt                                         >  2:660340/1‑289 (MQ=255)
                                                                                                                                                                                                                                         ttCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGt                                     <  1:771629/289‑1 (MQ=255)
                                                                                                                                                                                                                                               aCCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAAtttt                               >  1:411274/1‑289 (MQ=255)
                                                                                                                                                                                                                                                   cAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAg                           >  2:34164/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                aTCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATCATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGAc              >  2:220938/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                          cgaatAAATTTCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGc                                                                                <  1:730317/209‑1 (MQ=255)
                                                                                                                                                                                                                                                                          cgaatAAATTTCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGc                                                                                >  2:730317/5‑213 (MQ=255)
                                                                                                                                                                                                                                                                            tttAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGaaaa  >  2:662589/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                        
AAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGGTGTATGTCTGGCGAGCAAAAAATCCCGGACTCTTTTTCGCTTATATGGTGGCATATATCGGCTTCGGAATTTTATCTATCGGCATGATTGTTTATCTTATTTTCTATCGTTAATATTCCCCTCTCCAGTTAATTATTGAGAATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAA  >  NC_000913/2233671‑2234227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: