Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,214,658	0	A	G	26.0%	119.9 / 16.6	82	E538E (GAA→GAG)	rpoD	RNA polymerase, sigma 70 (sigma D) factor
Reads supporting (aligned to +/- strand):  ref base A (34/23);  new base G (2/18);  total (38/44)
Fisher's exact test for biased strand distribution p-value = 1.60e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAAC  >  NC_000913/3214520‑3214800                                                                                                                                           |                                                                                                                                               cAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGcc                                                                                                                                            >  2:557231/1‑143 (MQ=255) cAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGAAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATGTCATCGAGGAGACCCCCCTGGGCAACCCGCTGGACTCCGCAACCACCCAAAGcc                                                                                                                                            >  1:1085732/1‑143 (MQ=255)    gATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGCAAGCCtgc                                                                                                                                         >  2:193197/1‑143 (MQ=255)    gATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGACAGCCtgc                                                                                                                                         >  2:244379/1‑143 (MQ=255)       ccGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGCACCACATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCCACCACCGAGAGCCggcgcc                                                                                                                                      >  1:167182/1‑141 (MQ=255)         gCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACGACCCTCGAGCGGCCGCAGGATTCTGCGACCACCGAAAGCCggcgtgcg                                                                                                                                    >  1:677445/1‑143 (MQ=255)          cAAAGTGCTGAAGATCGCCAAAGAGCCAACCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCgg                                                                                                                                   <  2:604617/143‑1 (MQ=255)             aGTTCTGAAGATCTCCAACGAGCCACTCTCCATGGACACGCCGATCGGTGATGATGCAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCaa                                                                                                                                <  2:561270/143‑1 (MQ=255)                  tGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGCGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCac                                                                                                                           >  1:12083/1‑143 (MQ=255)                  tGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCGGCGTGCGGCCACggcg                                                                                                                           >  1:611682/1‑140 (MQ=255)                      aaTCGCCAAAGAGACAATCCCGATGAAAACGCCGATCGGTGATGATGAAGAGTAGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCGGCCGCTGGATTCTGCGACCACCGAAAGCCtgcgtgcgttgccgcacgcag                                                                                                                       <  2:149133/142‑14 (MQ=255)                       tgcGCCAGAGTGCCATTCTCCAGGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCAGCGAGGAGACCACCCTTGTGTTGCTGAATGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGt                                                                                                                      <  1:1176553/141‑1 (MQ=255)                       ctCGCTAAAGATCCTATCTCACTGGACACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTTTTTGTTTTTAAGGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGt                                                                                                                      <  1:4146/142‑1 (MQ=255)                       aTCGCCAAAGAGCCAATCTCCATGGAAACGCCGTTCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGt                                                                                                                      <  2:1120092/143‑1 (MQ=255)                         cGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGAATTTTAGCGTGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTgc                                                                                                                    >  2:1297453/1‑143 (MQ=255)                           ccAAAGAGCCACCCTCCATGGAAACGCCGATCGGTGATGATCCAGATTCGCCTCTCGGCGATTTCATCGAGGATACCACCCTCGAGCTGCTGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTgctg                                                                                                                  <  2:230192/143‑1 (MQ=255)                           ccAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGACAGCCTGCGTGCGGCAACGCCCGCCGTgctg                                                                                                                  >  2:1309470/1‑143 (MQ=255)                           ccAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCAGCGAGCGGCGACGCACGACATgccg                                                                                                                  >  1:78650/1‑141 (MQ=255)                           ccAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATGTCATCGAGGCTACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTgctg                                                                                                                  >  2:395998/1‑143 (MQ=255)                           ccAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGAAGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCAACCACGAGCTGCCGCTGGATCCTGCGACCACAGAAAGCCGGCGTGCCGCCACGCgcgaccggccg                                                                                                                  >  1:137817/1‑137 (MQ=255)                             gaagggctagtctcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCAGCGAGGATACCACCCTCTATTTTCTGCATGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTgctggc                                                                                                                <  1:408439/133‑1 (MQ=255)                             aaaGAGCCAATCTCCATGAAAACGCCTATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTgctggc                                                                                                                <  1:1083609/143‑1 (MQ=255)                              aaGAGCCTATCTCCATGGTTCCGCCGATCGGTTCTGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGTGCTGTCTAAGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTgctggct                                                                                                               <  1:834733/143‑1 (MQ=255)                               agtgtctagctcCATGGAATCGCCGGTCGGTGATGATGTGTATGCGCGTCTGGGGGCGTTCATCGAGGATCCCACCCTTGTGCTTTGTAATGATACTGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTgctggctg                                                                                                              <  1:794528/134‑1 (MQ=255)                               agagCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTTTTGTTGCCGAAGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTgctggctg                                                                                                              <  1:1059338/143‑1 (MQ=255)                                      agctcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCAGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGAc                                                                                                       <  1:665437/141‑1 (MQ=255)                                         tcCATGGATGCGCCGATCGGTGATTATGAAGTTTCTCATCTGGGGGATTTCATCGAGGATACCACCCTTGTGCTGCCGCTGGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACcgc                                                                                                    <  1:105348/143‑1 (MQ=255)                                         tcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACGCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACcgc                                                                                                    >  2:376508/1‑143 (MQ=255)                                          ccctcgAAGCGCCGATCGGCGATGATCAACATTCGCATCTGGGGGATTTCACCGAGGATCCCACCCTCGAGCTGCCGCTGGCTTCTGCGACCACCAATATCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACcgcg                                                                                                   <  2:628378/138‑1 (MQ=255)                                             tGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGACACCACCCCCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGt                                                                                                <  2:129575/143‑1 (MQ=255)                                                gaaCGCCGAGCGGTGGTGATGAGGATTCGCATCTGGGGGATGTCATCGAGGATACCACCCTCGATTTGTCTAAGGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGaa                                                                                             <  1:829453/142‑1 (MQ=255)                                                aagcGCCGATCGGTTATGATGAAGATTCGCATCTGGGGGATTGCATCGAGGATACCACCCTCGTGCTGCCGCAGGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGaa                                                                                             <  1:1340679/140‑1 (MQ=255)                                                aagcGCCGAGCGGGGATGCTGAAGAGTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCAGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGaa                                                                                             <  1:1227205/140‑1 (MQ=255)                                                 aaCGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCCCCGAAAGCCTGCGTGCGGCAACGACCCCCGGGCCGGCTGGCATgtaggcgtggcacc                                                                                            >  1:1071106/1‑130 (MQ=255)                                                      cGATCGGTGATGATGAAGATTCGCATCTGGGGGTTTTCATCGAGCATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTCCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCaaaa                                                                                       <  2:1111435/143‑1 (MQ=255)                                                         tCGGTGATGATGAAGATCCGCATCGGGGGGATTTCATAGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGtt                                                                                    <  2:1158478/143‑1 (MQ=255)                                                          cGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGCGACGCACAAGTTc                                                                                   >  2:893195/1‑143 (MQ=255)                                                                  atgaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATg                                                                           >  2:210297/1‑143 (MQ=255)                                                                   tgaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGc                                                                          >  2:259075/1‑143 (MQ=255)                                                                     caGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGt                                                                        <  2:84824/142‑1 (MQ=255)                                                                     aaGATTCGCATCCGCGGGCTTCCATCGATCTTACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGt                                                                        <  2:91123/143‑1 (MQ=255)                                                                         ttCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCg                                                                    <  2:1111029/143‑1 (MQ=255)                                                                         ttCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCg                                                                    <  2:64652/143‑1 (MQ=255)                                                                               tCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGCATCg                                                              >  2:1237468/1‑143 (MQ=255)                                                                                cTGGGGGATTTCATCGAGGATACCACCCGCGAGCTGCCGCTGGACTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCAAGACGTGCTGGCTGGCCTGCCCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGa                                                             <  2:1147384/143‑1 (MQ=255)                                                                                 tGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCATGCGTGCGGCACCGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGat                                                            >  2:1100378/1‑143 (MQ=255)                                                                                   ggggATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGatat                                                          <  1:1297453/143‑1 (MQ=255)                                                                                     ggATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGCAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGa                                                        >  2:560849/1‑143 (MQ=255)                                                                                     ggATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGa                                                        >  2:1108673/1‑143 (MQ=255)                                                                                        tttCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGGTGTCCGCACCGCCCGTGAAGCAAAAGGTCTGCGTATGCGTTTCGGTATCGATATGAcca                                                     >  2:329676/1‑143 (MQ=255)                                                                                         ttCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCCCCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAacac                                                    <  2:1087375/143‑1 (MQ=255)                                                                                             tCGAGGATCCCACCCTCGTGTTTCTGCAGGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGAc                                                <  1:990980/143‑1 (MQ=255)                                                                                             tCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGAc                                                >  2:1319618/1‑143 (MQ=255)                                                                                                aGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCgacgac                                             >  2:634228/1‑143 (MQ=255)                                                                                                     gccaccCTCGGGCGGCCGCTGGATGCTGCGACCACCGAAGGCCTGCGGGCGGCAACGCACGACGGGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCt                                        <  2:1004956/142‑1 (MQ=255)                                                                                                      ccaccCTCTGGCTGTCGCAGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTg                                       <  1:650751/143‑1 (MQ=255)                                                                                                       caccCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTCTGCGTGTGGGTATCGATATGAACAGCGACCACGCGGTgg                                      >  2:8894/1‑143 (MQ=255)                                                                                                       caccCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGGTTCGGTATCGATATGAACGCCGACTACACGCTgg                                      >  2:1353278/1‑143 (MQ=255)                                                                                                       caccCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCATGACCGCCCGTGAAGCAAAAGTTCTGCGTAGGCGTTTCGGTATCGCTAAGACCACCGACGACACGCCgg                                      >  1:93186/1‑143 (MQ=255)                                                                                                        accCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATCTGGACACCGACTACACGCTGga                                     >  2:362427/1‑143 (MQ=255)                                                                                                         cccTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGgaa                                    <  2:498393/143‑1 (MQ=255)                                                                                                            tCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAGAGTTCTGCGTATGCGTTTCGGTATCGATATGAGCACCGACTACACGCTGggagga                                 >  2:698578/1‑141 (MQ=255)                                                                                                                tctgctgATGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTgg                             <  1:482210/142‑1 (MQ=255)                                                                                                                ggtgctgatGGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTgg                             <  1:1116749/135‑1 (MQ=255)                                                                                                                gCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACGCCGACTACACGCTGGAAGAAGTgg                             >  2:1361107/1‑143 (MQ=255)                                                                                                                gCTGCCGAGGGATTGTGCGACCACCGACGGCCTGCGTGCGGCAACGCACGGCGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTgg                             <  2:1358205/143‑1 (MQ=255)                                                                                                                ccTGCCGCTGGATCCTGCGCCCCCCGCAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTgg                             <  2:12083/142‑1 (MQ=255)                                                                                                                  tGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACCTGCGGGCTGGCCTGTCCGCCCGTAAAGCAAACGTCCTGCGCCTGCGGTCCGGGGGCGAGATGAACACCGACTACCCGCTggccgagcgcggt                           >  1:1418320/1‑132 (MQ=255)                                                                                                                   gCCGCTGGATTCTGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCACGCGTGAAGCAAAAGCACTGCGTATGCGTTTCGGTATCGACATGAACACCGACTACACGATGGaagaagaaggga                          >  1:263780/1‑137 (MQ=255)                                                                                                                    ccGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTaa                         <  2:918716/143‑1 (MQ=255)                                                                                                                     tgccggATGCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTaaa                        <  1:1177091/139‑1 (MQ=255)                                                                                                                       cTGGATTCTGCGACCGCCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACa                      <  2:917810/143‑1 (MQ=255)                                                                                                                         ggATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTCACCGCGCGCGAAGCAAAAGTTCTCCGAAGCCCATCCGGAACCGAGATGAACACCCACTACACGCCGgaagaaacagatcaacaat                    >  1:741143/1‑130 (MQ=255)                                                                                                                          ggttCTGCGACCACCGATAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATGGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGtt                   <  1:930618/141‑1 (MQ=255)                                                                                                                           aTTCTGCGACCACGGAACGACGGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTc                  <  1:7510/143‑1 (MQ=255)                                                                                                                              ccgCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACTTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGAc               <  2:553019/141‑1 (MQ=255)                                                                                                                              cTGCGACGTCCGAAAGGCTGCGTGCGGCAATGCACGACGTGCTGGCTGGCCGGACCGCGCGTGAAGCAAAAGTTGTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGAc               <  2:1159381/143‑1 (MQ=255)                                                                                                                               tGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGAAGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGATGTGCGTATGCGTTTCGGTATCGATATGAACACCGAATACACGCTGGAAGAAGTGGGTCAAAAGTTCGACg              >  2:635904/1‑143 (MQ=255)                                                                                                                                  gACCACCGGGAGCCGGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTa           <  1:949522/143‑1 (MQ=255)                                                                                                                                      accGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGCTACCcg       >  1:266997/1‑143 (MQ=255)                                                                                                                                        cGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAACGTTCTTCGTCTGCGTTTCGGTATCGCTGTGCACCCCGCCTCCACGCTGACGCAAGTGGGTAAACCGGTCGACGCTCCCcgcg     >  1:257329/1‑143 (MQ=255)                                                                                                                                         gAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGAGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACCTTACCcgcgc    >  1:833563/1‑142 (MQ=255)                                                                                                                                          aaaGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCGTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACGACACGCGGGAAGAAGTGGGTAAACAGTTAGGCGTTAACGGCGaa   >  2:870689/1‑143 (MQ=255)                                                                                                                                           aaGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAAc  <  1:395998/143‑1 (MQ=255)                                                                                                                                           |                                                                                                                                               CAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAAC  >  NC_000913/3214520‑3214800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑