Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,184,542 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,184,5420CA98.9% 275.6 / NA 89P1100Q (CCG→CAG) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (45/43);  minor base G (0/1);  total (45/44)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAG  >  NC_000913/4184402‑4184681
                                                                                                                                            |                                                                                                                                           
tCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGc                                                                                                                                           >  2:1394461/1‑143 (MQ=255)
   ggCGCTTACACGCCGTCGCCAGCCTGGTGACACGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCACCCCGATCGAAGATATGCCTTACGATGACAACGGTACGCCGGTAGACATCGTACTGAACCAGCTgg                                                                                                                                        <  1:1144480/143‑1 (MQ=255)
   gccGGTTATATGCCGTATCGAGCCTGGTGACAAGATTGCAGGTCTTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATAGGCCTTACGATGAAAACGGTACGCTGGTAGACATCGTACTGAACCAGCTgg                                                                                                                                        <  1:484738/141‑1 (MQ=255)
       gTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGGTATGCCTTACGAGGACAACGGTACGCCGGTAGGCGTCGGACTGAACCAGCTGGGCGt                                                                                                                                    >  2:198485/1‑143 (MQ=255)
        ttAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATACCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTa                                                                                                                                   >  1:1197842/1‑143 (MQ=255)
            aCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCgc                                                                                                                               >  1:1212147/1‑142 (MQ=255)
              gCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACGGtct                                                                                                                             >  2:1150867/1‑143 (MQ=255)
              gCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGtct                                                                                                                             >  2:129725/1‑143 (MQ=255)
               ccGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGAGCGAAGATACGCCTTCCGATGACAACGGCACGCCGGTAGACATCGTACTTAACCAGCTGGGCGTACCAtctc                                                                                                                            >  2:407264/1‑143 (MQ=255)
                cGTATCGAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCg                                                                                                                           <  2:1394629/143‑1 (MQ=255)
                cGTACCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCg                                                                                                                           <  2:36599/143‑1 (MQ=255)
                  tATCCAGCCTGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGGTTAATTTCTAAGATCACCCCGAGCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTAt                                                                                                                        <  1:526994/143‑1 (MQ=255)
                   aTCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGCACCAGCTGGGCGTACCGCCGCGTAt                                                                                                                        >  2:497734/1‑143 (MQ=255)
                    tCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATg                                                                                                                       >  2:1193065/1‑143 (MQ=255)
                      cAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGaa                                                                                                                     >  2:549799/1‑143 (MQ=255)
                         ccTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGCACCAGCTGGGCGTACCGTCTCGTATGAACAt                                                                                                                  >  1:271410/1‑143 (MQ=255)
                           tGGTGACAAGATGGCAGGTCGTGACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGTCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTACGAACAtgt                                                                                                                <  1:591524/143‑3 (MQ=255)
                           tGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCg                                                                                                                >  2:362741/1‑143 (MQ=255)
                            ggTGACAAGATGGCAGGTCGTCAGGGTAACAAGTGTGTAATTTCTAAGATCAACCCGATCGAAGATCTGCCTTACGATGAAAACGGTACGCCGGTACACATCGTACCGAACCAGCTGGGCGAACCGTCCCGAACGAACacagg                                                                                                               >  1:517844/1‑139 (MQ=255)
                               gACAAGATGGCTGTTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCa                                                                                                            <  1:1537/143‑1 (MQ=255)
                               gACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTcg                                                                                                            >  2:1323480/1‑142 (MQ=255)
                               gACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGAGCGAAGATATGCCTTACGATGAAAACGGTACGCCGGAAGACAGCGTACTGAACCAGCTGGGCGCACCGCATCGTATGACCaacaggca                                                                                                            >  1:1232260/1‑136 (MQ=255)
                                 cAAGATGGCAGGTCGTCACGGTAACCAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGCAAACGGTACGCCGGTAGACATAGTACTGAACCAGCTGGGCGTACCGTCTCGTATGCACCTCGGGCAGa                                                                                                          >  1:1427316/1‑143 (MQ=255)
                                  aaGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTCTGAACATCGGTCAGAt                                                                                                         >  1:486982/1‑143 (MQ=255)
                                  aaGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAt                                                                                                         >  1:50808/1‑143 (MQ=255)
                                      tGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTc                                                                                                     <  1:398897/143‑1 (MQ=255)
                                      tGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTc                                                                                                     <  2:499702/143‑1 (MQ=255)
                                        gCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCgc                                                                                                   >  2:228079/1‑142 (MQ=255)
                                                tcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGCTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGGATGAACATCGGTCAGATCCTCGAACCCCAcc                                                                                           >  1:1091541/1‑143 (MQ=255)
                                                 gaCGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCt                                                                                          <  1:1367279/142‑1 (MQ=255)
                                                 cACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCt                                                                                          >  1:1227071/1‑143 (MQ=255)
                                                  aCGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATTGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTg                                                                                         <  2:64292/143‑1 (MQ=255)
                                                  aCGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCCGATCCTCGAAACCGACCTg                                                                                         >  2:255212/1‑143 (MQ=255)
                                                   cGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGTCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTgg                                                                                        <  1:252463/143‑1 (MQ=255)
                                                   cGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTgg                                                                                        <  2:1354163/143‑1 (MQ=255)
                                                    ggTAACAAGGGTGGAATTTCTAAGCTCCACCCGATCGAAGATATGCCTTACGATGAGAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTggg                                                                                       <  1:127329/143‑1 (MQ=255)
                                                         catggggtgaagttCGAAGATCGACCCGATCGAAGATATGCCTGACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGGACATCGGTCAGATCCTCGAAACCCACCTGGGTATgg                                                                                  <  1:491785/131‑1 (MQ=255)
                                                         cAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGCACATCGGTCAGATCCTCGAAACCCACCTGGGTATgg                                                                                  <  2:934601/143‑1 (MQ=255)
                                                         cAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCCGGGTATgg                                                                                  >  1:171007/1‑143 (MQ=255)
                                                            gggTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTACGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTg                                                                               >  2:1232752/1‑143 (MQ=255)
                                                             tgtgtCATTTCTAAGCTCCACCCGCTCCAAGAGATTCCTTACCATGTAAACGGTACTCCGGTAGACATCGTACTGACCCAGCTGGACGTACCGTCTTGTATGAACATCGGTCAGATCCTCGAAACCCATCTGGGTATtgctgc                                                                              <  1:801440/142‑1 (MQ=255)
                                                              gtgtAATTTCTAAGATCAACCCGCTCGAAGATATGCCTTACGATGAAAACAGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTAGGAACACCGGACAGATACTCGACACCCACCAGGGAATGGCAGCg                                                                             >  1:989339/1‑143 (MQ=255)
                                                                 tAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                          <  2:1027306/143‑1 (MQ=255)
                                                                  gaTTGCTAAGATCAACCCGATCGAAGATATGCGTTACGAGGAGATCGGTACGCCGGTAGACATCGGACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTACGAAAg                                                                         <  1:985913/142‑1 (MQ=255)
                                                                  attttCTAAGATCACCCCGCTCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACAGCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAg                                                                         <  2:310161/141‑1 (MQ=255)
                                                                  atcttatAAGATCAACCCGATGGAAGATGTGCCTTACGATGAAAGAGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAg                                                                         <  1:1190887/137‑1 (MQ=255)
                                                                  aaTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAg                                                                         <  2:1246932/143‑1 (MQ=255)
                                                                        tAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCg                                                                   >  2:968746/1‑143 (MQ=255)
                                                                           gacccaCCCGATCGAAGAGATGCCTTACGACGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCg                                                                <  1:1167241/138‑1 (MQ=255)
                                                                            aTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGa                                                               <  2:1092718/143‑1 (MQ=255)
                                                                             tCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCGGCGAAGGGTCTCGGCGAc                                                              >  1:1188346/1‑143 (MQ=255)
                                                                              cAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACa                                                             <  1:1193065/143‑1 (MQ=255)
                                                                               aaCCCGATCGAAGATATGCCTTACGAGCACAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGCCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACaa                                                            <  2:184369/143‑1 (MQ=255)
                                                                                 cccGATCGAAGATATGCCTTACGATGAAAACGCTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCGCGGCATGGCGGCGAAAGGTATCGGCGACacgc                                                          >  1:756886/1‑140 (MQ=255)
                                                                                        gAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGAGACACAATCAACGc                                                   >  1:66837/1‑143 (MQ=255)
                                                                                         aaGATATGCCTTACGATGAAAACGGTGCGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGcc                                                  >  2:702252/1‑143 (MQ=255)
                                                                                         aaGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGcc                                                  <  2:1093067/143‑1 (MQ=255)
                                                                                         aaGAGATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGcc                                                  <  2:580630/143‑1 (MQ=255)
                                                                                           gggaggCCCTAAGAAGATAACGGTACACCGGGAGACGTCGTACTGAAGCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCAt                                                <  2:287103/138‑1 (MQ=255)
                                                                                            atatGCCGTACGACGAAAACGGTAGCCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACACCGGTCAGATCCTCGAACCCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATg                                               <  2:553355/143‑1 (MQ=255)
                                                                                                gCCTTACGATGAACACGCTACGCCGGTAGACATCGTACTGAACCAGATGAGCGTACCGTCTCGTCTTAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGa                                           <  2:1129629/143‑1 (MQ=255)
                                                                                                   ttACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAGACACACCTGGGTATGGCTGCGAAAGGTATAGGCGACAAGGTGAACGCCGTGCGGaagc                                        >  1:866768/1‑141 (MQ=255)
                                                                                                   ttACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCCCATGGGTACGGCTGCGAAAGGGATCGGCGACAAGACCAACGCCATGCCGAAAc                                        >  2:986703/1‑143 (MQ=255)
                                                                                                   ttACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCACCGCCATGCCGacac                                        >  1:209415/1‑143 (MQ=255)
                                                                                                   ttACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAc                                        >  2:1075009/1‑143 (MQ=255)
                                                                                                   ttACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGAGACCGCCATCGTCGGCAAGATCAACGCCATGCTGaaaa                                        >  2:1363617/1‑142 (MQ=255)
                                                                                                         tGAAAACGGTACGCCGGTGGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAACGGTATCGGCGACAAGATCAACGCCATGCTGAAACagcagc                                  >  2:696545/1‑143 (MQ=255)
                                                                                                          gAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAgcagca                                 <  2:1236653/143‑1 (MQ=255)
                                                                                                           aaaaCGATACGCCGGTAGACATCATACTGCACCACGACTCCGCACCTGCTCGTATGAACATCGGTCAGACCCTCCAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaa                                <  1:299197/143‑1 (MQ=255)
                                                                                                             aaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCGTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaaga                              >  2:783353/1‑143 (MQ=255)
                                                                                                             aaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaaga                              >  2:1287803/1‑143 (MQ=255)
                                                                                                              aCGGTACGCCGGTAGACAGAGTACAGAACCAGCGGGGCGTACCGTCTCGTAGGAACATCGGTCAGAGCCGCGAAACCCACCTGGGTATGGCTGCGAAGGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaagaa                             <  2:61074/143‑1 (MQ=255)
                                                                                                                   aCGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcgc                        <  2:50808/143‑1 (MQ=255)
                                                                                                                     gCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTCAAACAGCAGCCAGAAGTCGCGa                      >  2:291560/1‑143 (MQ=255)
                                                                                                                     gCCGGTAGACATCGTACTGAACCAGCTCGGCGTACCGTCCCGAATAAACATCGATCAGAACCACAAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGa                      >  2:442934/1‑143 (MQ=255)
                                                                                                                        ggTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTCTGGCTGCGAAGGGTATCGGCGACAAGATCAACGCCATGCCGAAACCGCAGCCAGAAGCcgcgcacc                   >  2:588571/1‑139 (MQ=255)
                                                                                                                           aGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTACGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgc                <  1:1232752/143‑1 (MQ=255)
                                                                                                                           aGACAACGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgc                <  2:465235/143‑1 (MQ=255)
                                                                                                                             aCATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGACACACCAGCAAGAAGTCGCGAAACTgcgc              >  2:974588/1‑143 (MQ=255)
                                                                                                                             aCATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcgc              <  2:961932/143‑1 (MQ=255)
                                                                                                                                tCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGaa           >  2:1139450/1‑143 (MQ=255)
                                                                                                                                   tACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCCGATCCTCGAAACCCACCTGGGTATGGCGGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAGACAGCAGCAAGAAGTCGCGAGACTGCGCCAATTc        >  1:789196/1‑143 (MQ=255)
                                                                                                                                   tACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTc        <  2:1339993/143‑1 (MQ=255)
                                                                                                                                    aCTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCa       <  2:1227071/143‑1 (MQ=255)
                                                                                                                                     cTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCAt      <  1:1287803/143‑1 (MQ=255)
                                                                                                                                      tGAACCATCTGGCCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATc     <  2:1227036/143‑1 (MQ=255)
                                                                                                                                       ggaCCAGCTGGGCGTAGCGTCTCGAAAGCACTAATGGCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATcc    <  2:263918/141‑1 (MQ=255)
                                                                                                                                        aaCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACACGATCAACGCCATGCTGAAACAGCCGCACGAAGTAGCGAAACTGCGCGAATTCATCCa   >  1:1176981/1‑143 (MQ=255)
                                                                                                                                         aCCGGCTGGGCGTGCCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAg  <  1:1394461/143‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
TCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAG  >  NC_000913/4184402‑4184681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: