| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,361,533 | 0 | A | T | 20.9% | 75.2 / 11.3 | 47 | I226N (ATC→AAC) | rhmR | putative DNA‑binding transcriptional regulator for the rhm operon |
| Reads supporting (aligned to +/- strand): ref base A (19/15); new base T (0/9); total (22/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.48e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTTTAATTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACGGCGAATACGCGCCAGTT > NC_000913/2361391‑2361674 | tgtgcttttttGGTCGGGTCATGAGGTGTTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACGTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGACTTGCCCGCCAGATAATCACGGTATTCTTCATTGa < 2:448811/137‑1 (MQ=255) tttAATTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGAt > 1:19171/1‑143 (MQ=255) tttAATTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGAt > 2:569037/1‑143 (MQ=255) ttAATTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGAtt < 2:431796/143‑1 (MQ=255) tAATTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGAttt < 2:733032/143‑1 (MQ=255) ttGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGc < 2:529270/143‑1 (MQ=255) tGGTAGGGTCATGATGTTCTCCATGGTTAGGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCg < 1:357997/143‑1 (MQ=255) ggTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCACCAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATCACCACGGGCTGCTGCATTGACTGGTAGGCgg > 2:314056/1‑143 (MQ=255) cgTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCgg < 1:323590/142‑1 (MQ=255) gTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCGGATAATCCCGGCTTTCTTCCTTCGTTTGTAAACgga > 1:10665/1‑142 (MQ=255) atgatgTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAG‑‑CGacc > 1:465996/1‑143 (MQ=255) gatgTTCTCAATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTCTTCTTCATTGATTGGTAAGCGGGTAC‑‑CGaccac > 2:570510/1‑143 (MQ=255) tgTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATGTCCCTGGCGCAGGCACTGGCCTTGCCCGCCAGATAATCACGGTACTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCg > 2:285809/1‑143 (MQ=255) ttGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCTATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAg < 1:483200/143‑1 (MQ=255) gTTATGGGGGTGGTAAGTGAAAGGGACTTGTCCATCGCAACAGACGTGAAATATTCCTGGCGCAGGCTATTGCCTTGCCCGCCAGATAAGCACGGTATTCTTCTTTTTTTTTTAATGGTGCGG‑‑CGACCACCGAGATCGCAGCg < 2:306511/143‑1 (MQ=255) gCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTc < 1:701672/143‑1 (MQ=255) aGTCAATGGGACTTTTCCATCCCATCAGACGTGAATTATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCTTTTTTTTTTAATGATACGG‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTg < 2:42360/143‑1 (MQ=255) gTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGACCGCAGCGGGAAGCTCGTCATTgg > 2:828815/1‑143 (MQ=255) tacaGGGACTTTCCCAGCCGAACAGACCTGAAATATCCCTCGCGCAGGCGATTGTCTGGCCCGTCAGATAAGGACGGTAGTGTCCTTTGTTTTTTAA‑‑TGGTACGGCGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCg < 2:777367/140‑1 (MQ=255) aGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTa < 1:816187/143‑1 (MQ=255) gggACTGTTCCATCCCAACAGACGTTAAATATCCCTGGCGCAGGCAATGGCCTTGCCGGCCAGATTATCATGAGATTCTTCTTTTTTTTGTAATGATACGG‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTaa < 2:695150/143‑1 (MQ=255) ccATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCCCGGTCTTCTTCACTGCTCGGCAAGAGGGTTC‑‑CGTCCTCCCAGGTCGCCGCGGTGAGCCCGTTCCTGGCGGCACACACAGgcg > 2:834482/1‑143 (MQ=255) tCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAg < 1:422423/143‑1 (MQ=255) ccgctgccGTGTAATATCGCTGGCGCAGTCAATGGCTTTGCCGGCCAGACCATCACCGTATTCTTCTTTGTTTTTTAA‑‑GGGTACGGCGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGa < 2:591904/136‑1 (MQ=255) cAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGa > 1:346834/1‑143 (MQ=255) ataggcGTGAAATAGCCGTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGaca < 1:777694/138‑1 (MQ=255) aCAGACGTGAAATATCCCGGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTTGTTATTGGCGTTAAACACCGGCGCAGCGaca < 1:442619/143‑1 (MQ=255) tGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTCGCGTTCAACACCGGCGCAGCGGCTCGACggg > 2:600524/1‑142 (MQ=255) tGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGCAAGCTCGTTATCGGCGTTAAACCCCGGCGCAGCGCCGCAACGGa > 2:600531/1‑143 (MQ=255) gagcgatcgggggCTCAGGCAATGGCCTTGCTCGGCAGATAATCACGGTATTCTTCATTTTTTTTTAAGGGTGCGG‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGAc < 2:730737/132‑1 (MQ=255) gaattttCCCTGGCGTTGTCTTTGGCCTTGCCCGCCAGATAATCACGTTATTCTTCTTTTTTTTTTAATGAGGCGG‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGAc < 2:212104/137‑1 (MQ=255) gAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTGATTGATTTGTAAGCGGATAC‑‑CGACCACCGAGATCGCAGCGGGAAGCTCGTTATGGGCGTTCGACTCTGGCGCAGCGCCACAGCGGAc > 2:357710/1‑143 (MQ=255) gAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAACCACGGTCTTCTTCATTGCTTCGTACGCGGGTCC‑‑CCACCACCCCGCCCGCAGCAGCACGCCCGCCACCGGCGGTCAACACCCGCGCacccgcgccgctcga > 2:535360/1‑129 (MQ=255) ggCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTCAGCCCCGGCGCAGCGACCCCACGGTCATCGGCGtcct > 1:37435/1‑140 (MQ=255) gcgcAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGT‑AGCCCGGTAGTGGGGGTTAACAACGGCGCAGGGACACAACGGACGATGGGGTAATCt > 1:772680/1‑143 (MQ=255) ggCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGGAAGCTCGTTATTGGCGTTAAAAACAGCTACTTCGTCACAACGGACATCGGCGTAATCTTCGc > 1:432875/1‑143 (MQ=255) ctgcccTCGCCCGCCAGATGCTCACGCTTTTCTTCTTTTTTTTTTAAGGATACGG‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGtt < 2:264586/139‑1 (MQ=255) tGGCCTTGTCCGCCAGATAATCACGGTGTTCTTCTTTTTTTTTTAATGATACGG‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTg < 2:644922/143‑1 (MQ=255) gCCTTGCCCGCCAGATAATCACGGTTTTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGcccctgcc > 1:616821/1‑137 (MQ=255) gCCAGGTAAGCACGGTCTTCTTCATTGCTTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCa < 1:221336/143‑1 (MQ=255) gATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGccc < 1:569037/143‑1 (MQ=255) cGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACGGCg > 1:566556/1‑143 (MQ=255) ttcttcAATGATTTGTAAGCGGGTAG‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACGGCGAATAc < 1:522932/143‑1 (MQ=255) cttcATTGATTTGTAAGCGGGTGC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACGGCGAATAcgc < 2:400020/143‑1 (MQ=255) aTTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACGGCGAATACGCGCCa < 1:517446/143‑1 (MQ=255) aTTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCACGTTATTAGCGTTAACCACCCGCCCCGCGCCACCACCGACATGGGGGTAAGCCTCGCCGTTGTCATAGCTCCAGGCGTTACGGCGCAAAcgccaca > 2:339341/1‑139 (MQ=255) ttGATTTGCAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGCAAGACCGTTATTCGCGCTAAACACCCGCCCAGCGACACAACGGACCTCGGCGTAATCCTCGCAGCTCTCATAGCTCCAGGCCTGCAGGCGGATACACGccgg > 2:705743/1‑141 (MQ=255) gATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTGGTCATCGCTCCAGCCCTGACGGCGAATACGCGCCAGtt > 1:451390/1‑143 (MQ=255) | GTTTAATTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACGGCGAATACGCGCCAGTT > NC_000913/2361391‑2361674 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |