Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,049,885	T→C	intergenic (+134/‑13)	polA → / → spf	5' to 3' DNA polymerase and 3' to 5'/5' to 3' exonuclease/Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,049,885	0	T	C	100.0%	217.1 / NA	71	intergenic (+134/‑13)	polA/spf	5' to 3' DNA polymerase and 3' to 5'/5' to 3' exonuclease/Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/38);  total (33/38)

GCACTAAGATTCGCCTGAACATGCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGAT  >  NC_000913/4049745‑4050024                                                                                                                                               |                                                                                                                                            gcACTAAGCTCCGCCTGAACATGCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGGGATGGATATTAGAAATTCCTATTCAACAACTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCaa                                                                                                                                           <  1:768999/143‑1 (MQ=255)     tAAGATTCGCCTGAACATGCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTACTATGCAACAACTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGCAC‑AAAAAAGCGCAAGGtt                                                                                                                                       >  1:169352/1‑143 (MQ=255)             gCCTGAACATGCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAAGTCCTATGCAACAACTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGt                                                                                                                               >  2:428805/1‑143 (MQ=255)              ccTGAACATGCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTG‑AAAAAAAAGTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGGTAGTCGCGTa                                                                                                                              >  1:467129/1‑143 (MQ=255)               cTGAACATGCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTG‑AAAAAAAATTACAAAAAGTGCTTTCTGTACTGAAC‑AAAAAAGAGCAAAGTTAGTGGGGTAg                                                                                                                             >  1:74296/1‑143 (MQ=255)                 caaCATGCCTTTTCTCGTAAGTAAGCAACATAAGCTGTCAAGTTTTGTGATAGCGATTAGAAATTCCTATGCAACAACTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAggg                                                                                                                           <  2:409430/142‑1 (MQ=255)                     aTGCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACa                                                                                                                       >  1:378724/1‑143 (MQ=255)                         cTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGGGCAATGTTAGTCGCGTAGGGTACagag                                                                                                                    >  1:450014/1‑143 (MQ=255)                             ttgcgcaagtaagCAACATAAGCTGTCACGTTTTGTGATGGCTATGAGAAAGCCCTAGGCAACAACTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAg                                                                                                               <  1:38578/137‑1 (MQ=255)                              ttCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGa                                                                                                              >  2:110341/1‑143 (MQ=255)                               tagtaagtaaGAAACATAAGCTGTAAAGTATTGTGATGGCTAATAGAAATTCCTATGCAACAACAA‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGAt                                                                                                             <  2:759058/141‑1 (MQ=255)                               tCGTAAGTAAGCAACATAAGCTGTCACGTTTAGGGATGGCTATTAGAAATTCCTATGCAACAACTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGAt                                                                                                             <  1:801502/143‑1 (MQ=255)                                  taagtaagCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGtt                                                                                                          >  1:651373/1‑143 (MQ=255)                                  taagtaagCAACAGAAGCAGTCACGTTTTATAATGGATAGTAGAAAGTCATATGAAACAAATA‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGtt                                                                                                          <  2:600244/143‑1 (MQ=255)                                  gaagtaagCAAGACGAGCTGTGACTATTGGTGATGGCTAATAGAAAGACGTAGGCAATGACTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGtt                                                                                                          <  2:298452/142‑1 (MQ=255)                                   aagtaagCAACATAAGCTGTCAGGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTG‑AAAAAAAAGTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTc                                                                                                         <  2:41978/143‑1 (MQ=255)                                    agtaagCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCt                                                                                                        <  1:536234/143‑1 (MQ=255)                                     gtaagCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTa                                                                                                       <  1:695193/143‑1 (MQ=255)                                      taagCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTAt                                                                                                      >  1:824132/1‑143 (MQ=255)                                       aagCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTGCTATGCAACAACTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATc                                                                                                     >  1:5261/1‑143 (MQ=255)                                          cAACATGAGCTGTCACGTGTTGTGATAGCTATTAGAAATTCCTATGCAACAACTG‑AAAAAAAATTACAAAAAGTGCTCTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCttt                                                                                                  <  2:707276/143‑1 (MQ=255)                                           aaCATAATGTGTCACGTTTTGTTATGGCTATTAGAAATTCCTATGCAACAACT‑‑TAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCa                                                                                                <  2:545184/143‑1 (MQ=255)                                             cATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCATAGGGTACATCGGTACGCTGTGCTATcgtcaag                                                                                               >  1:578996/1‑137 (MQ=255)                                               tAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTACGATGTTCTATCTTTCAGAc                                                                                             >  2:167951/1‑143 (MQ=255)                                               gcaGCTGTCACGTTTGGTGATGGCTATGAGAAGTTCCTATGCAACAACAG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGAc                                                                                             <  2:368574/141‑1 (MQ=255)                                                      gcACGATTTGAGATAGCTAGTAGAAATTCCTATGCAACAACAG‑AAAAAAAAGTACAAAAAGTGCTTTCTGAACCGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTAc                                                                                      <  2:571858/142‑1 (MQ=255)                                                       cACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACt                                                                                     <  1:371022/143‑1 (MQ=255)                                                        aCGTTTTGAGATGGCTATTAGAAATTCCTATGCAACAACTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AGAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACtt                                                                                    >  1:521773/1‑143 (MQ=255)                                                           ttttGTGATGGCTATTAGAAATTCCTATGCAACAACTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCAc                                                                                 <  1:440918/143‑1 (MQ=255)                                                           tgttGTGACGCATATTAGAAATTTGTATGCAACAACTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACCGAAC‑AAAAATGCGCACAGTTTGTCGCGTAGCGTACAGAGGTAAGATTTTCTATCTTTCAGACCTTTTACTTCAc                                                                                 <  2:333303/141‑1 (MQ=255)                                                                tgATGGCTATTAGAAATTCCTATGCAACAACTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAAt                                                                            >  1:761624/1‑143 (MQ=255)                                                                  aaggCTAGTAGAAATTCCTAAGCAACAACTGAAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATc                                                                           <  1:491743/141‑1 (MQ=255)                                                                    ggCTATTAGAAATTCCTATGCAACAACTG‑AAAAAAAATTACAAAAAGTGCTTTCTGGACTCAGC‑AAAAAAGCGCAAAGTTAGTCGCGTAGGGTAAAGAGGTAAGAAGTACTATCTTTCAGACCATTTACTTCACGTACTAGGa                                                                        >  1:79421/1‑143 (MQ=255)                                                                    ggCTATTAGAAATTCCTATGCAACAACTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACATTTTACTTCTCGTAATCgaa                                                                        >  1:153171/1‑141 (MQ=255)                                                                      cTATTAGAAATTCCTATGCAACAACTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGAtt                                                                      <  2:222298/143‑1 (MQ=255)                                                                         ttAGAAATTCCTATGCAACAACTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTTACATAATCGGATTTgg                                                                   >  2:783092/1‑143 (MQ=255)                                                                         tgaGAAATTCCTGCGCAACAACTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTgg                                                                   <  2:203372/141‑1 (MQ=255)                                                                          tAGAAATTCCTATGCAACAACTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGc                                                                  >  1:752594/1‑143 (MQ=255)                                                                            gAAATTCCTATGCAACAACTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGATCTTTTACTTCACGTAATCGGATTTGGCTg                                                                >  1:400025/1‑143 (MQ=255)                                                                             aaaTTCCTATGCAACAACTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGa                                                               >  1:748633/1‑143 (MQ=255)                                                                                    tATGCAACAACTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAGGAtttt                                                        >  1:472654/1‑143 (MQ=255)                                                                                    tATGCAACAACTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATAtttt                                                        <  1:428805/143‑1 (MQ=255)                                                                                     aTGCAACAACTG‑AAAACAAATTACAAAAAGTGCTTTCTGAACTGACC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCTGGTCTTTTTAGTCACTGAATCGGATTTGGCTGAATATTTTa                                                       <  2:703837/143‑1 (MQ=255)                                                                                      tGCAACAACTT‑AAAAAAAATTACAAAAGGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGGCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAg                                                      <  1:506624/143‑1 (MQ=255)                                                                                       gCACCAGCTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGTGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAgc                                                     <  2:425595/143‑1 (MQ=255)                                                                                         aacaacTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGATCTATCTGTAAGGGTCAGTGCGGCACGTAATCGGATTTGGCTGAATATTTTAgccg                                                   >  2:176744/1‑143 (MQ=255)                                                                                            aacTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACATTGGACTTCACGTCATCGGATTTGGCTGAATATTTTAGCCGccc                                                >  2:508351/1‑143 (MQ=255)                                                                                               tG‑AAAAAAAATTACTAAGAGTGCGCTCTGAACTGAAC‑AAAGAAGTGCATTTTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCcag                                             <  2:253256/143‑1 (MQ=255)                                                                                               tG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGCGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATCTTAGCCGCCCcag                                             >  2:167082/1‑143 (MQ=255)                                                                                                   aaaaaaaTTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGCTGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCcagtca                                          >  1:514219/1‑143 (MQ=255)                                                                                                     aaaaaTTACAAAAACTGCTTTCTCGACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCcagtcagt                                        <  1:520228/143‑1 (MQ=255)                                                                                                             cgaaaaGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTg                                <  1:412817/141‑1 (MQ=255)                                                                                                              aaaaaGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTgg                               >  2:811751/1‑143 (MQ=255)                                                                                                              aaaaaGTGCTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTgg                               >  1:12318/1‑143 (MQ=255)                                                                                                               agaaGTGGTTTTTGGACCGAAAAAAAAAAGAGCAAGGTTAGTCCCGTATGTTCCGGAGTTAGGATGTTTTTCCTTTCGGACCTTTTCCTTCCCGTATTCGAATTTGGCTGAATA‑TTTAGCCGCCCCAGTCAGTAATGACTggg                              <  2:741463/141‑1 (MQ=255)                                                                                                                 atgTGCTTGGTGAACTGAAC‑AAAAAAGAGCAAACTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCATTACTGACTGGGGc                            <  2:386298/141‑1 (MQ=255)                                                                                                                     gCTTTCTGAACTGAAC‑AAATAAGACCAAAGTTATGCTCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCATGTATTCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGttt                        <  2:7562/143‑1 (MQ=255)                                                                                                                      cTTTCTGAACTGAAC‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGtttt                       >  1:65476/1‑143 (MQ=255)                                                                                                                         tCTGAACTGAAC‑AAAAAAGCGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTa                    <  1:307039/143‑1 (MQ=255)                                                                                                                            gaactgaac‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGATCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTg                 <  2:400025/143‑1 (MQ=255)                                                                                                                             aagtgaac‑ATAAAATAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTgg                <  2:551477/140‑1 (MQ=255)                                                                                                                             aacgggac‑AAGAAAGAGCAAAGTTGGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACGTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTgg                <  2:155785/137‑1 (MQ=255)                                                                                                                                tgaac‑AAAAAAGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGCATCTTTTACCCGCCCCACTCAGTACTGACTTGGGCGTTGTTTATTGGGca             >  2:540268/1‑142 (MQ=255)                                                                                                                                        aaaaGAGCAAAGTTAGTCGCGTAGGGTACAGATTTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCgacagaaa      >  2:519810/1‑143 (MQ=255)                                                                                                                                        aaaaGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCgaacgaaa      >  1:297458/1‑143 (MQ=255)                                                                                                                                        aaaaGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTGTCTATTGGGCgaaagaca      >  1:325047/1‑141 (MQ=255)                                                                                                                                        aaaaGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACGGGGGCGGTTTTTGTTGGGCgcaagaaa      >  2:584117/1‑143 (MQ=255)                                                                                                                                         aaaGAGCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGaaagaaaa     <  2:697716/143‑1 (MQ=255)                                                                                                                                          aaGAGCAAAGTTAGTCGCGTAGTGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGaaagaaaag    <  1:760740/143‑1 (MQ=255)                                                                                                                                           agagCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGaaagaaaaga   <  2:609187/143‑1 (MQ=255)                                                                                                                                            gagCAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGAt  <  1:144498/143‑1 (MQ=255)                                                                                                                                               |                                                                                                                                            GCACTAAGATTCGCCTGAACATGCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTG‑AAAAAAAATTACAAAAAGTGCTTTCTGAACTGAAC‑AAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGAT  >  NC_000913/4049745‑4050024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑