Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,020,956	A→G	intergenic (‑37/‑182)	sulA ← / → sxy	SOS cell division inhibitor/CRP‑S‑dependent promoter expression factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,020,956	0	A	G	100.0%	116.6 / NA	44	intergenic (‑37/‑182)	sulA/sxy	SOS cell division inhibitor/CRP‑S‑dependent promoter expression factor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/21);  total (23/21)

CACTGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGAT  >  NC_000913/1020817‑1021089                                                                                                                                            |                                                                                                                                      cACTGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATgtgcag                                                                                                                                    <  2:303107/143‑1 (MQ=255)    tGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATgtgcagtac                                                                                                                                 >  1:354305/1‑143 (MQ=255)    tGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATgtgcagtac                                                                                                                                 >  1:289881/1‑143 (MQ=255)          gCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACCTAATCAATCAAACCCCTGGGAGATACTGGATGCATCTGCAGTACAGCCaa                                                                                                                           >  2:336633/1‑142 (MQ=255)          gCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGGACATAATAAATCCAGCCCCTCTGAGCTACTGGATGGATGAGCAGTACATcccc                                                                                                                           >  2:336520/1‑141 (MQ=255)                   tAGTGGTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGAcaacaa                                                                                                                  <  2:781264/143‑1 (MQ=255)                     gtgtTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAg                                                                                                                <  2:326471/143‑1 (MQ=255)                       gtTTGCCGTAGAGACACGCGCAATTTCACTTGCTGCGGATGAGGACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGAt                                                                                                              <  1:195983/143‑1 (MQ=255)                          ttCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCaa                                                                                                           <  1:235119/143‑1 (MQ=255)                               tAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATTATCATTCCCGCCCCTCTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCATa                                                                                                      >  1:195994/1‑143 (MQ=255)                                agagACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAATACATCCAGTGACAACACAGATCAACCCCAt                                                                                                     >  1:149226/1‑143 (MQ=255)                                 gagaCACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTAtt                                                                                                    <  2:79378/143‑1 (MQ=255)                                    acacGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTc                                                                                                 >  1:256368/1‑143 (MQ=255)                                    acacGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGACTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATCTTc                                                                                                 >  1:701074/1‑143 (MQ=255)                                     gacGCGCAACTTTGCTGGGTGCGGAGGAGAACGACGAAGAACGATGGGCATAGCCTGAAGTGTACATAAGCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCg                                                                                                <  2:58733/142‑1 (MQ=255)                                              ttttACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGCTCAACCCTATTATCGGAAagaggc                                                                                       >  2:201260/1‑141 (MQ=255)                                                ttACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTTACAACAAAGATCAACCCTATTTTCGTAAAGAGCCTc                                                                                     >  1:135550/1‑143 (MQ=255)                                                    ttGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGACAGAGCCTCGCaa                                                                                 >  1:732858/1‑143 (MQ=255)                                                        tgcGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAGttt                                                                             >  1:742853/1‑143 (MQ=255)                                                         gtgggtGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAAtttt                                                                            <  2:306780/138‑1 (MQ=255)                                                         gcGGATGAGCACGACGAAGAACGATGTGCATAGCCTCAAGTGTACATAATCAATCTAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGCTCAACCCTATTTTCGGAAAGAGCCTCGCAAAtttt                                                                            <  2:224001/143‑1 (MQ=255)                                                           ggATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATCATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGt                                                                          <  2:446858/143‑1 (MQ=255)                                                           ggATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGCCATACACGATCTGCCCTATTTTCGGAAAGAGCCTCGCAAATTTTGt                                                                          <  1:163266/143‑1 (MQ=255)                                                            gATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTCGCGGAAAGAACCTCGCaagcattgtc                                                                         >  2:282482/1‑135 (MQ=255)                                                                agaACGACGAAGAACGAGGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTAACAACCAAGATCAACCCTATCTTCTGAAAGAGCCTCGCAAATTTTGTCGTTg                                                                     <  1:364711/143‑1 (MQ=255)                                                                 gaACGACGAAGAACGATGTGCATAGCCTGGAGTGTACATAAGCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTgg                                                                    <  2:582297/143‑1 (MQ=255)                                                                 gaACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTgg                                                                    >  1:559705/1‑143 (MQ=255)                                                                 aaaCGAAGAAATACGTGGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGCCAACAAAGATCAACCCTATTTTCGGAACGAGCCTCGCAAATTTTGTCgccgt                                                                    >  2:841296/2‑139 (MQ=255)                                                                        gaagaaCGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTAAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAGGAGCCTCGCAAACTTTGTCGTTGGTGatgat                                                             >  1:268863/1‑139 (MQ=255)                                                                                     cATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATATTCGGAAAGAGCCTCGCAAAATATATCGTTGATGACGGGAAAACATAAATTa                                                >  2:128426/1‑143 (MQ=255)                                                                                       tAGCCTGAGGGGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTGCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAAt                                              <  2:535645/143‑1 (MQ=255)                                                                                             gAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTGTGTCGTTGGTGACGCGACAACCTAAATCAATCTTGcc                                        >  2:505373/1‑143 (MQ=255)                                                                                                   tACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGGGCAGTACATCCAGTGACAACGAAGATGAACCCTATTTTCGGAAAGAGCCGCGCAAGATGTGGCGGTGGTGACGGGAGAACGTAAATTAATCTTGCCGCttga                                  >  2:605286/1‑141 (MQ=255)                                                                                                     gataagtcgtcCAGGCCCGGTGAGGTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCATCCCGATTCTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGa                                <  2:747369/134‑1 (MQ=255)                                                                                                     cATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCGAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGa                                >  1:140363/1‑143 (MQ=255)                                                                                                        aatcaatcCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATa                             <  1:436191/143‑1 (MQ=255)                                                                                                        aatcaatcCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGCTGGTGACGGGAAAACACCAATTAATCTTGCCCCTTAAGAATa                             >  2:252504/1‑143 (MQ=255)                                                                                                          tcaatcCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATCAg                           >  1:467540/1‑143 (MQ=255)                                                                                                            gatcCAGCCGGTGGGAGTTACTGTGTGGATGTGCAGTACATCCAGTGCCAAGAAAGATCAGCCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAGTTAAGCTCGCCCCTTAAGAATAAGtt                         <  2:105983/142‑1 (MQ=255)                                                                                                              ttcAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAAAAAAGATCAAACATATTTTCGGAAAGAGCCTCGAAAATTTTGTAGTTGCTGATGGGAAAAAATAAATTAATATAGAACGTTAAGAATAAGtaaa                       >  2:276242/3‑140 (MQ=255)                                                                                                                      gtgagAGCTACTGTAGGGATGTGCGGTACATCCAGTGACAACAAAGATGAACCCTATGTTCGGAAAGAGCCTCGCAAAGTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTc               <  2:701074/139‑1 (MQ=255)                                                                                                                      cTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTc               <  2:256368/143‑1 (MQ=255)                                                                                                                         tgAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAAACCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTa            <  1:850156/143‑1 (MQ=255)                                                                                                                             ttACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTa        >  1:457409/1‑143 (MQ=255)                                                                                                                             ttACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTa        <  1:419686/143‑1 (MQ=255)                                                                                                                                 tGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGCAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCTTAGTTAACgg    >  1:776669/1‑143 (MQ=255)                                                                                                                                   gaTGGACGTGCAGTACATCATGTGACAGCATAGCTCACCCTTATTTTCTGAAATAGCCTCGCAATTTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGAt  <  2:412332/142‑1 (MQ=255)                                                                                                                                            |                                                                                                                                      CACTGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGAT  >  NC_000913/1020817‑1021089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑