Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,196,305 C→T R395C (CGT→TGT)  icd → isocitrate dehydrogenase, e14 prophage attachment site, tellurite reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,196,3050CT98.2% 156.0 / NA 56R395C (CGT→TGT) icdisocitrate dehydrogenase, e14 prophage attachment site, tellurite reductase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (33/22);  total (33/23)
Fisher's exact test for biased strand distribution p-value = 4.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGG  >  NC_000913/1196166‑1196442
                                                                                                                                           |                                                                                                                                         
ggACAAAGTAAATCCTGGCTCGATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTc                                                                                                                                        <  1:576560/143‑1 (MQ=255)
 gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGGAGGCGCAATCAACGCGAAAGCCGTCACCTATGACTTCGAgtgttt                                                                                                                                       >  1:25843/1‑141 (MQ=255)
   cAAAGGAAGTCCTGCCTCTATTATGCGTGGCGCTCAGATGATGCTGCGCCACAGGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTGCGAGTGTCTga                                                                                                                                     <  2:122389/143‑1 (MQ=18)
       gttattCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTgatgga                                                                                                                                 <  2:32547/138‑1 (MQ=255)
       gTACATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTgatgga                                                                                                                                 <  2:813710/143‑1 (MQ=255)
       gTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTgatgga                                                                                                                                 >  2:800828/1‑143 (MQ=255)
           aTCCTGGCTCTATGATTCTCTCCGCTGAGATGATGCTTCGCTGCATGGGTTGGACTGGAGCGGCTGAGTTGATTGTTATAGGTTTGGAAGGTGTGTTTAGTGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGgc                                                                                                                             <  1:610820/143‑1 (MQ=255)
            tCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGCAGGCGCAATCACCGCGAAAACCGTAACCTCTGACTTCGAGTGTCTGATGGATGgcg                                                                                                                            >  2:853099/1‑143 (MQ=255)
            tCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGgcg                                                                                                                            >  1:336839/1‑143 (MQ=255)
               tGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTa                                                                                                                         >  1:485696/1‑143 (MQ=255)
                     tattatTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAActgc                                                                                                                   >  2:144535/1‑143 (MQ=255)
                        tgttgtcgccGCTGGGAGGATGCTGGGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGa                                                                                                                <  2:107344/135‑1 (MQ=18)
                           tctctcCGCTGAGATGATGCTGCGCCACATGGGTTGTACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTTCTGAAAt                                                                                                             >  2:47368/1‑143 (MQ=255)
                               tcCGCTGAGATGATGCTGCGCCACCTGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTc                                                                                                         >  2:385093/1‑143 (MQ=255)
                               tcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTc                                                                                                         >  2:14784/1‑143 (MQ=255)
                               tcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCAGAAATGTTc                                                                                                         >  2:14869/1‑143 (MQ=255)
                                 cGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCACAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCag                                                                                                       <  1:796143/143‑1 (MQ=255)
                                  gccgagaTGATTCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCaga                                                                                                      <  2:460800/140‑1 (MQ=255)
                                   cTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAGTGTTCagag                                                                                                     >  2:220442/1‑143 (MQ=255)
                                      agaTGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTTAGTGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGttt                                                                                                  <  1:623467/143‑1 (MQ=255)
                                       gatgatGCTGCTTCATATGGGTTGGACCTAATTTGTTGTTTTTATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTg                                                                                                 <  1:48442/143‑1 (MQ=17)
                                                gcgcCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGa                                                                                        >  1:589177/1‑143 (MQ=255)
                                                 cgcCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGat                                                                                       >  1:267651/1‑143 (MQ=255)
                                                     agttGGGTAGGGGCGAATCGGCTGTCTTAATTGTTAAAGGTATGGAAGGTGCAATCAATGAGAAAACCGGTAGCTATTGCTTGGTGTGTGTGATGGATGGCGATAAACGGCTGAAATGTTCAGAGTTTGGTGAGGCGatcatc                                                                                   <  2:757803/140‑1 (MQ=12)
                                                        tGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGATTGGTGACGCGATCATCGaa                                                                                >  1:650530/1‑143 (MQ=255)
                                                               gACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGt                                                                         <  1:5201/143‑1 (MQ=255)
                                                                  cGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGCCGCGATCATCGAAAACATGTAAt                                                                      >  1:175400/1‑143 (MQ=255)
                                                                    aaGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGc                                                                    >  1:418372/1‑143 (MQ=255)
                                                                           gtatctAATTCTTGAAGGTATGTAAGGCGCAATCAATGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGtt                                                             <  2:426580/138‑1 (MQ=17)
                                                                               ttAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGtt                                                         >  2:316859/1‑143 (MQ=255)
                                                                               ttAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCAAAAACATGTAATACCGTAGTGTGtt                                                         >  1:127620/1‑143 (MQ=255)
                                                                                  aTTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTaga                                                      >  1:462385/1‑141 (MQ=255)
                                                                                  aTTGTTAAAGGGATGGACGGCGCAATCAACGCGAAATCCGTATCCTATGACTGCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTaaa                                                      <  2:211647/143‑1 (MQ=18)
                                                                                         aaGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATttatta                                               >  2:424996/1‑143 (MQ=255)
                                                                                           agcaTGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAAc                                             <  1:455962/140‑1 (MQ=255)
                                                                                               tGGAAGGCGCAATCATCGCGAATTTCGTATTCTATGACTTGGAGTGTCTGATGGATGGCGCGAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGa                                         <  1:1081/143‑1 (MQ=21)
                                                                                               tGGAAGGCGCAATCAACGGGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGa                                         <  2:762407/143‑1 (MQ=255)
                                                                                                 gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGGGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGCGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAGATTTGTTAACGGGAGc                                       >  1:638297/1‑143 (MQ=25)
                                                                                                      cgcAATCAAGGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAAc                                  <  2:127752/143‑1 (MQ=255)
                                                                                                         aaTCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGAAGCGTAACGCt                               >  2:237645/1‑143 (MQ=255)
                                                                                                         aaTCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGATGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCt                               >  2:795364/1‑143 (MQ=255)
                                                                                                              cagtgAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTACCGGGAGCGTAACGCTCCCgt                          >  2:728107/5‑143 (MQ=255)
                                                                                                              aCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGCTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCgt                          <  1:186954/143‑1 (MQ=255)
                                                                                                              aCGCCAAAACCGTACCCTATGACTTCGAGTGTCTGATGGATGGCGCTAACCTGCTGAAATGTTCAGAGTTTGGGGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCgt                          <  1:515144/143‑1 (MQ=25)
                                                                                                               cgcgAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAGACATGTACTGCCGTAGTGTGTTACATTTATTAACGGAAGCGTAAGGcgcgcggt                         >  2:611390/1‑136 (MQ=21)
                                                                                                               cgcgAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCgtt                         <  1:779238/143‑1 (MQ=255)
                                                                                                                  gAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTAGCgttgat                      >  1:659393/1‑141 (MQ=255)
                                                                                                                  gAAAACCGTAACCTATGACTTCGAGTGTCTGATCGCTGGCGCTAAACTGCTGAATTGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTCACGCTCCCgttgtt                      >  1:588633/1‑143 (MQ=25)
                                                                                                                   aaagCCGTGACCTATGTCTTGCAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGGGTCTGGTGACTCGATCTTAGCCAATTTCCATTGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGttt                     <  2:180638/143‑1 (MQ=255)
                                                                                                                   aaaaCCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGttt                     >  1:762263/1‑143 (MQ=255)
                                                                                                                   aaaaCCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCGgtcgtgt                     >  1:378152/1‑141 (MQ=255)
                                                                                                                    aaaCCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGtttt                    <  1:462557/143‑1 (MQ=255)
                                                                                                                        cGTAACCTATGACTTCGAGTGTCTGATAGATCTCTCCAATCTGCTGCACTGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGCTCttttttta                >  2:703356/1‑141 (MQ=25)
                                                                                                                                     ttCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACg   >  1:642152/1‑143 (MQ=255)
                                                                                                                                     ttCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTCGTTAAATTTATTACCGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCGAACg   >  1:12619/1‑143 (MQ=255)
                                                                                                                                      tCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACgg  >  1:760243/1‑143 (MQ=255)
                                                                                                                                      tCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACgg  >  1:603781/1‑143 (MQ=255)
                                                                                                                                           |                                                                                                                                         
GGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGG  >  NC_000913/1196166‑1196442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: