BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,178,956	T→C	13.1%	S282P (TCT→CCT)	tolC →	transport channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,178,956	0	T	C	13.1%	221.1 / 2.7	99	S282P (TCT→CCT)	tolC	transport channel
Reads supporting (aligned to +/- strand): ref base T (21/58); new base C (13/3); total (34/65)
Fisher's exact test for biased strand distribution p-value = 6.53e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

CTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAAC‑AGGCACAGTACAACTTTGTCGGT‑GCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAG  >  NC_000913/3178722‑3179194

cTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACCCTG‑GATTTAACGGCTTTTACCGGGGTTTCTGACACCCCttt                                                                                                                                                                                                                                                >  1:1014620/1‑238 (MQ=255)

  gcgcCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTTCACCGGGGATTCTTACCACCCTtata                                                                                                                                                                                                                                              >  1:235356/1‑239 (MQ=255)

  gcgcCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTtata                                                                                                                                                                                                                                              >  1:530784/1‑239 (MQ=255)

             ccGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTTTGCACCCTCTTTTAGCgggttggaa                                                                                                                                                                                                                                   >  1:506852/1‑232 (MQ=255)

              cGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGCAACCCCTTTTAGCGGGTCGaaaa                                                                                                                                                                                                                                  >  1:971279/1‑239 (MQ=255)

                   actcctATCCGGAACTTGCTGGGCTGAATTGCAAAAACTTAAAAACCGAAAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCgt                                                                                                                                                                                                                             <  2:180736/239‑1 (MQ=255)

                   actactATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGCTTTCTGACACCCCTTATAGCgttctcttatacaca                                                                                                                                                                                                                             >  1:1003104/1‑225 (MQ=255)

                   actactATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAAcc                                                                                                                                                                                                                                >  1:65807/1‑236 (MQ=255)

                      actgcccGGAACTGGCTGCGCTGAATGTAGAAAACTTTAAACCCGACCAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCgtggt                                                                                                                                                                                                                          <  1:540028/234‑1 (MQ=255)

                      actaTCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCgtggt                                                                                                                                                                                                                          <  1:1014207/239‑1 (MQ=255)

                          tCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGAAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCCACCTGGCGTTTTTACACACCTTATAACGGATCCAAAAacccaggcgccc                                                                                                                                                                                                                      >  2:810561/1‑230 (MQ=255)

                             ggAACTTGCTGCGGTGAATGTCGAAAACTTTAAAACCGAACACCCACAGGCGGTTAACGCGCGTCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTg                                                                                                                                                                                                                   <  2:677008/239‑1 (MQ=255)

                               aaCTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTGAACGGCTTCTAGCGCGATTTTTGACACCCCTCATACAGGTTGGAAAAACCgcgggggccgcagt                                                                                                                                                                                                                 >  2:1023472/1‑229 (MQ=255)

                               aaCTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCC‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACCCTG‑GATTTAACGGCTTCTACCGGGATTTCGTACACCCCCTTAAACGGTTCGAAAACCCgtgggcggcctgg                                                                                                                                                                                                                  >  2:27560/1‑229 (MQ=255)

                                 cTGGCTGCGGGTAATGGCTAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTa                                                                                                                                                                                                                <  1:129504/238‑1 (MQ=255)

                                  tGGCGGCGCGGAATGACGAAAACTTTAAAACCGACAACCCACGGCCGGTTAACGCGCTGCGGAAAGAAGCCGAAAAACGCAACCTGGCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAACTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTAcc                                                                                                                                                                                                              <  2:235356/239‑1 (MQ=255)

                                   ggCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTAccc                                                                                                                                                                                                             <  1:27560/239‑1 (MQ=255)

                                    ggcggcttGAATGTCGAAAACTTAAAAACCGACAACCCCCAGCCGGTTAACGGGCTGCTGAAAGAAGCCGAAAAACGCAACCTGGCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTAccc                                                                                                                                                                                                             <  2:65807/231‑1 (MQ=255)

                                      tGCTCTGAATGTCAAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGt                                                                                                                                                                                                          <  1:679997/239‑1 (MQ=255)

                                           tGAATTTCGAAAACTTTAAAACCGACAAACCCCAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAACCGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGGG‑GGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGAc                                                                                                                                                                                                     <  2:731929/239‑1 (MQ=255)

                                            gAATTTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGAc                                                                                                                                                                                                     <  1:974927/238‑1 (MQ=255)

                                             aaTGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCg                                                                                                                                                                                                                                           <  1:201194/199‑1 (MQ=255)

                                               tggtaaaaaTTTTAAAACCTAATAACCCCAGCCGGTTACCGCGCTGCGGAAAGAAGCCGAAAAACGCAACCTGTCGGTGGTACAGGCACGTTGGTGCCAGGA‑CCT‑GGCACG‑CGAGCAAATTCGCCAGGCGCAGGTTGGTCATTTCCCGACTCTG‑GATTTAACGGCTTCTAACGGGATTGTTGACACCTCTTATCGCGGTTCGAAACCCCGTGGTGCCGCTGGTACCCAGTATGACGATa                                                                                                                                                                                                 <  2:711024/234‑1 (MQ=255)

                                                ggcGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAg                                                                                                                                                                                                <  1:929564/237‑1 (MQ=255)

                                                gTCGAAAACTTTAAACCCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAg                                                                                                                                                                                                <  1:109883/239‑1 (MQ=255)

                                                       aCTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACCCTG‑GATTTAACGGCTTCTACCCGGGTTTGTGCCACCCCTTATATAGGTTGGCAAAc                                                                                                                                                                                                                                 >  2:201194/1‑199 (MQ=255)

                                                          ttAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGc                                                                                                                                                                                     <  1:338474/239‑1 (MQ=255)

                                                          taaaacccaaacacccACATCCGGTTAACGCCCTGCTAAAAGAAGCCGAAAACCGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCAATTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAATCCCGTGGTGCCGCTGGGACCCAGTATGACGATAGC‑AATATGGGc                                                                                                                                                                                     <  2:824283/225‑1 (MQ=255)

                                                               accagcAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGGGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACGCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGaa                                                                                                                                                                                <  1:449193/234‑1 (MQ=255)

                                                               aCCGACAAACCCCCTGCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCGGGCGCTGTTACAGGCCCGCTTGACCCGGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGaa                                                                                                                                                                                <  2:672110/239‑1 (MQ=255)

                                                                   aCAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAAGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTACCACCCCTTATAGCGGTTCGAAAACCCGTGCTGCCGCTTGTCCCCAGTCTGTCGATAGC‑CATATAGACCACAaaaac                                                                                                                                                                            >  2:1038158/1‑238 (MQ=255)

                                                                       tccACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGAG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGa                                                                                                                                                                                                   <  1:285698/212‑1 (MQ=255)

                                                                         ccccggcGGTTAACCCCCTGGTGAAAGGAGCCGAAAAACGAAACCTATCGCTGTTACAGGCACGCTTGGGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATGCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGc                                                                                                                                                                      <  2:1078026/233‑1 (MQ=255)

                                                                           ccgCCGTTTACCGCGCTGCTGAAAGAAGCCAAAAAACGCAACTTGTCGCTGTGACGGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGGGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCt                                                                                                                                                                    <  2:530784/237‑1 (MQ=255)

                                                                            aGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCCCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AAAATTGGCCAGGACAAAGTTGGCCT‑g                                                                                                                                                                  >  1:1016508/1‑239 (MQ=255)

                                                                              ccGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑Aata                                                                                                                                                                                          <  1:717980/214‑1 (MQ=255)

                                                                                   tAACGGCCTGCTGAAAGAAGCCGAAAAACGGCACCGGTCGCTGTTACAGGCACGCTTGAGCCAGGG‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTtct                                                                                                                                                           <  1:409863/239‑1 (MQ=255)

                                                                                   tAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTTGAAAACCCGTGGTGCCGATGCGACCCCGGTAGACGCTAGCAAAAAAGGGCAAAAACAAA‑TTGGCCT‑AAGCt                                                                                                                                                              >  2:340355/1‑236 (MQ=255)

                                                                                        cgtgcccgAAAAAAGCCCAAAAACCCACCCTGGCCCCTTTAAAGGCCCGCTTGGGCCAGGA‑CCT‑GGCGGG‑GGAGCAAATTCGCCAGGCGCAGGATGGTCCCTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTg                                                                                                                                                      <  2:1016508/232‑1 (MQ=255)

                                                                                        cgcTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGGATTCTGACACCCCTTATAACGGTTCGAAAACCCCTGGTGCCGCTTGTACCCACTCTTACGATAGC‑ACTATGGGCCCAAACactgtcctattaacaccttaacca                                                                                                                                                       >  2:717980/1‑216 (MQ=255)

                                                                                          ctgctgAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCCGGA‑CCT‑GGCGCG‑CGAGCAACTTCGCCAGACGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTAACCGGAGTTCTTCCACCTCTTATAGCGGTTCTACAACCCGTGGTCGCGCTTGTCCACACACTGACGATAAA‑CAAATGGGCCAAAACAAAGATGGCCG‑GGGCTTCTCCCTggc                                                                                                                                                    >  2:508240/1‑237 (MQ=255)

                                                                                           tgctgAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCCGCACCCCCTTTTATCGGTTCCAAAACCCGTGGTGCCGCTGGGACCCCGGATGAGGATAGA‑CAAATGGGCCCAAAAAAAGATGGcccgggcgttcgcgtgcgg                                                                                                                                                    >  2:1000157/1‑222 (MQ=255)

                                                                                            gctgAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGTTACCCAGTATGACGATAGC‑AATATGGGCCAGAACCAAGTTTGCCT‑GGGCTTCTCGCTGCCgg                                                                                                                                                  >  1:721595/1‑238 (MQ=255)

                                                                                            gctgAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCG‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGAGTCTACCGGGGTTTCTGATACCCCTTATAGCCGTTCGAAAAACCGCGGGGGCGCTGGGACCCAGTTTGGCCAAAGA‑CATAAGGGCCCGAAAAAAATTGGCCT‑GAGCTTCTCGCTGACgc                                                                                                                                                  >  2:108830/1‑238 (MQ=255)

                                                                                                 aagcagCCCAACACCGCAACCTTGCGCTGTTACAGGCACGCTTGAGCCAGGC‑CCT‑GG‑GCGTCGAGCAAATTCGGCAGGCCAGGGATGGTTATTTCCCGACTTTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTATTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTAt                                                                                                                                             <  2:606194/239‑1 (MQ=255)

                                                                                                 aagaagCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTAt                                                                                                                                             <  1:247173/239‑1 (MQ=255)

                                                                                                     agCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGGTTTCTTACACCTCTTTTATCGGTTCGAAAACCCGCGTGTCCCCTGGTCACCAGTATGACACAAGC‑AATATAGGCCAGACCCAAGTTGGCCT‑GGCGTTTTCGCTGCCCATTTTTCAgg                                                                                                                                         >  2:1093202/1‑239 (MQ=255)

                                                                                                     agCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTATCa                                                                                                                                           <  2:598822/237‑1 (MQ=255)

                                                                                                     agCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGGATGACGATAGC‑AATATGGGCCGGAACAAAGTTGGCCT‑TAGCTTCTCGCAGCCGATTTAtacag                                                                                                                                         >  1:342184/1‑235 (MQ=255)

                                                                                                       ccGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAAACAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTATCAGGGc                                                                                                                                       >  1:640340/1‑239 (MQ=255)

                                                                                                        cGAAAATCGCAACCTGTCGCTGTTACAGGGACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTATCAggg                                                                                                                                        <  1:108830/237‑1 (MQ=255)

                                                                                                          aaaaaCGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTTCCGCTGGGACCCCGTATGGCGATAGA‑AATATGGGCCAGAAAAAAATTGGCCT‑GAGCTTCTCCCGGCCGGTTTATccaggggga                                                                                                                                    >  2:653450/1‑231 (MQ=255)

                                                                                                           aaaaCGCAACCTGTGGCTGTTAAGGGCACGCTTGAGCCAGGC‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGGTGGATGGGATCTTCCCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTAGAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑CAGCTTCTCGCTGCCGATTTATCAGGGCGGa                                                                                                                                    <  2:763125/238‑1 (MQ=255)

                                                                                                                            ttttcggggccagcgtgggCCAGGACCCT‑GGCG‑G‑CAGGCAATTTCGGCAGGCGCAGGCTGCTTACTTACCGACGTTG‑GATTTAACGGCTTTGACCGGGATTGCTGACACATCTTAGGGTGGTTCGAAAACCCGTGTGGCCGGTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAGGTGGGCCT‑GAGGGTCTCGCTGCCGATGTATCAGGGCGGAATGGTTAACTCGCAGGt                                                                                                                   <  2:856108/220‑1 (MQ=255)

                                                                                                                            tttgaCAGGCCCGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTGACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGt                                                                                                                   <  1:1038158/234‑1 (MQ=255)

                                                                                                                            tGTTACAGGCGCGGTTGAGCCAGTC‑CCT‑GGCGTG‑CGATCAAATTCGCCGGGGGGAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCGCTTATAGCGGTGCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGt                                                                                                                   <  2:42219/238‑1 (MQ=255)

                                                                                                                              ttACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGGAACCTCTTATACAGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGCATGACCATAGC‑AATATTGGGCAAAGCAAAGTTGCACT‑TAGGTTCTACCTTCCCGTTTTTCAGGGCGGAATGTTTTACACGAAGGtaca                                                                                                                >  2:967848/1‑236 (MQ=255)

                                                                                                                                   ggCCCGCTTTGGCCCGGC‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATTGTCCCTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAAC‑Agg                                                                                                          <  2:1014620/239‑1 (MQ=255)

                                                                                                                                     cccGCTTGGGCCAGGA‑CCT‑GGCGTG‑CGAGAAAATTCGCCAGGCGGAGGATGGTCCCTTACCGACTTTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAAC‑AGGca                                                                                                        <  2:263427/237‑1 (MQ=255)

                                                                                                                                      ccggttGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCCCCAGGCGCAGGAGGGTCACGTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCGCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAAC‑AGGca                                                                                                        <  1:631005/234‑1 (MQ=255)

                                                                                                                                      acgtgtgggCCAGGT‑CTG‑GGCGGG‑CGAGCAAATTGGCCAGGGGCAGGAGGGTGTATGACCGCATCGG‑GATTTAAGGGCTTCTACGGGGATTGCTGAACCCCCTGATAGCGTTTCGAAAACCCGATGTGCCGCTGGTACCCAGTAGGACGATAGC‑AATATGGGCCAGAACAAAGTGGGCCT‑GAGCTTCTCGCTGCCGATTTATCAGGGAGGAATGGTTAACTCGCAGGTGAAAG‑AGGca                                                                                                        <  2:660214/230‑1 (MQ=255)

                                                                                                                                      aCGGTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAAC‑AGGca                                                                                                        <  1:653450/238‑1 (MQ=255)

                                                                                                                                      aCGGTTGAGCCAGGA‑CCG‑GGCGCG‑CGAGCAAAGTCGCCATGCGCAGGATTGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAAC‑AGGca                                                                                                        <  1:625006/238‑1 (MQ=255)

                                                                                                                                      aCGCTTTAGCCAGGA‑CCTGGGGGTG‑CG‑TCAAATTCGCCAGGCGCAGGATGGTTCCTTACCGGCTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAAC‑AGGca                                                                                                        <  1:888135/238‑1 (MQ=255)

                                                                                                                                      aCGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAAC‑AGGca                                                                                                        <  1:1093202/238‑1 (MQ=255)

                                                                                                                                      aCGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAAC‑AGGca                                                                                                        <  1:926600/238‑1 (MQ=255)

                                                                                                                                        gCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGACAACCCGTGGTGCCGCTGGGACCCAGCATGACGATAGC‑AATATTGGCCAGAACACAGTTGGCCT‑CAGCTTCTCGCTGCCGATTTATCAGGGCGGAAAGGTTAACCAGAACGGGAAAC‑AGGCACAg                                                                                                     >  2:75158/1‑239 (MQ=255)

                                                                                                                                        gCTTGAGCCAGGA‑CCT‑GGCGCG‑AGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCCG‑GATTTAACGGCTTCTACCGGGATTCTTTACACCTCTTATAACGGGTCGTAAACCCGCGGTGACCCTGGTGCACACACTGACCTTAGC‑TAGATGGGCCCGAAACAACTTTGCCC‑CAGCATCTCCCCCCCCCTTTTACAGTGCGGAATGAGTGCCTCGCAGGTggacacagacacg                                                                                                      >  2:47437/1‑227 (MQ=255)

                                                                                                                                                ccctgcgccgggCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGGCGCTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGGCACCTCTTATAGCGGTTCGAAAACCCGTGGTGc                                                                                                                                                                                                                        <  1:318999/111‑1 (MQ=255)

                                                                                                                                                    a‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTTCCCCGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTTTCAGGGGGGAAGGGTTAACTCGCAGGTGAAAA‑AAGACCAGTAAAACATTGtg                                                                                         >  2:774260/1‑238 (MQ=255)

                                                                                                                                                          ggCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAAC‑AGGCACCGTACAACTTTGTCggg                                                                                      >  1:730207/1‑237 (MQ=255)

                                                                                                                                                                 cgAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGGGGTTCGAAAAACCGTGGGGCCCCTGGGACCCAGTATGACGATCGC‑AAAATTGGCCAGAAAAAACATGGCCT‑GAGCTTTTCGCCTCCGATTTATCAGGGCGGAAGGGGTACATTGACGGTGGAACAAGGCAC‑CTAAAACTTTGTCGGG‑GCCAgag                                                                              >  2:443801/1‑237 (MQ=255)

                                                                                                                                                                    tgcaaTTCCCCGGGCGCAGGATTGTCCCTTTCCGA‑ACTGTGATTTAACGGCTTCTTCCGGGATTTATTACACCTCTTATAGCGGTTCGAAAAGCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAAC‑AGGCACAGTACAACTTTGTCGGT‑GCCAGCGAg                                                                            <  2:565038/235‑1 (MQ=255)

                                                                                                                                                                    gCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAAC‑AGGCACAGTACAACTTTGTCGGT‑GCCAGCGAg                                                                            <  1:296223/238‑1 (MQ=255)

                                                                                                                                                                        aTTCGCCAGGCCCGGGATGTGCCCTTACCGACTGGT‑GATTTAACGGCTTCCTCCGGGATTTCTGACACCACTTAGAGCGGTTCGAAAACCCGGTGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAAC‑AGGCACAGTACAACTTTGTCGGT‑GCCAGCGAGCAACt                                                                       <  2:952343/239‑1 (MQ=255)

                                                                                                                                                                             ccAGGCGCAGGATTGTCACTTACCGACTCTG‑GATTTAACGGCTCCTACCGGTATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAAC‑AGGCACAGTACAACTTTGTCGGT‑GCCAGCGAGCAACTGGaaa                                                                  <  1:481064/239‑1 (MQ=255)

                                                                                                                                                                              cAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGCTTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAAC‑AGGCACAGTACAACTTTGTCGGG‑GCCAACGAGCAACTGGaacg                                                                 >  1:952343/1‑237 (MQ=255)

                                                                                                                                                                                   ggtgggttgtccatcaCCGGCTCTG‑GCTTTAACGGCTTCTACCGGGATTTCTGACCACTTTTAGAGCGGTTCGAAAACCCGTGGTGCCGGTGTTGCCCAGGATGACGATAGC‑AATCGGGGCCAGAACAAAGTTGGCCT‑GAGCGTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAAC‑AGGCACAGTACAACTTTGTCGGT‑GCCAGCGAGCAACTGGAAAGTGcc                                                             <  2:1085381/223‑1 (MQ=255)

                                                                                                                                                                                   gcAGGATGGTCCCTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTATTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAAC‑AGGCACAGTACAACTTTGTCGGT‑GCCAGCGAGCAACTGGAAAGTGcc                                                             <  1:178588/238‑1 (MQ=255)

                                                                                                                                                                                   gcAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAAC‑AGGCACAGTACAACTTTGTCGGT‑GCCAGCGAGCAACTGGAAAGTGcc                                                             <  1:774260/238‑1 (MQ=255)

                                                                                                                                                                                       gATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAAC‑AGGca                                                                                                        <  1:862871/192‑1 (MQ=255)

                                                                                                                                                                                          tgTCACTTACCGACTCTG‑GATTTAACCGCTTCCTCCGGGATTTCTGACACCTCTTATAGGGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAAC‑AGGCACAGTACAACTTTGTCGGT‑GCCAGCGAGCAACTGGAAAGTGCCCATCGTa                                                      <  1:145150/237‑1 (MQ=255)

                                                                                                                                                                                            tcctttaccctgcctG‑GTTTTAACGGCCTCTACCGGGCTTTCTGACCACTTTTAGAGCGGTTCGAAACCCCGGGGGGCCGCTGGGACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTGGGCCT‑GAGCTTCTCGCGGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAAC‑AGGCACAGTACAACTTTGTCGGT‑GCCAGCGAGCAACTGGAAAGTGCCCATCGTAGc                                                    <  2:506852/225‑1 (MQ=255)

                                                                                                                                                                                            tcccTTCCCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAAC‑AGGCACAGTACAACTTTGTCGGT‑GCCAGCGAGCAACTGGAAAGTGCCCATCGTAGc                                                    <  2:1007624/235‑1 (MQ=255)

                                                                                                                                                                                            tCACTTCCCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAAC‑AGGCACAGTACAACTTTGTCGGT‑GCCAGCGAGCAACTGGAAAGTGCCCATCGTAGc                                                    <  1:1084651/238‑1 (MQ=255)

                                                                                                                                                                                                 tACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGGGCCGCTGGTAACCAGTATGACGCAAGC‑AATAAGAGCCAGAACAAAGTGGGCCG‑GAGCTTCTCCCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAAC‑AGGCACACTACAACTTTGGCGGT‑GCAAGCGGACAAAGAGAAAGTACCCaccagaacgggggg                                              >  2:391509/1‑226 (MQ=255)

                                                                                                                                                                                                   ccGTCTCTG‑TATTTAACGCCTTCGTACGGGATTTCTCACAACCCTTAGTGCGGTTAGAAAACCCGTGGTGCCGGTGGTACCCAGGTTGACGATAGC‑AATATGGGCCAGAACAAAGTTGGACT‑GAGCTTCCCGCTGCCGATTTATCAGGGCGGAAGGGTTAACTCGCAGGTGAAAC‑AGGCACAGTACAACTTTGTCGGT‑GCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCa                                            <  2:430268/239‑1 (MQ=255)

                                                                                                                                                                                                          tG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCCTGGTGCCGCTGTTACCCCATAAGACCGTTGC‑AATATTGGCCCGAACAAAGTTGGCCTGGAGC‑TCTTCCGGCCCACTTATACGGCCGGGACAGTTTACTCGCAGGGGAAAC‑CCTCCAAGTACAACTTT‑TGGCTGGCCAGCGGACACATGGACAGTGCCCCTCgacccgtactccaacaccg                                      >  2:440796/1‑220 (MQ=255)

                                                                                                                                                                                                                 tAACGGCTTCTTCCGGGATTTCTGACACCTCTTAAAGCGGTTCGAAACCCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAAC‑AGGCACAGTACAACTTTGTCGGT‑GCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTtcc                               <  1:391509/239‑1 (MQ=255)

                                                                                                                                                                                                                   aCGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGc                                                                                                                                                                                     >  1:107875/1‑90 (MQ=255)

                                                                                                                                                                                                                   aCGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGc                                                                                                                                                                                     <  2:107875/90‑1 (MQ=255)

                                                                                                                                                                                                                   aCGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAATTGGCCT‑GAGCTTCTCGCTGCCGATTTATTAGGGCGGAATGGTTAACCCCCAGGTGAAAC‑AGGCACAGTACAACTTTGTCGGG‑GCCCACGGGCAAACGGAAACGGCCCACCGTAggcgcgggcaaaacgggcggtccc                              >  2:49301/1‑215 (MQ=255)

                                                                                                                                                                                                                        ttCTACCGGGATTTCTGACCCCCTTTATAGCGGTTCGAAATCCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAAC‑AGGCACAGTACAACTTTGTCGGT‑GCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTc                         <  1:29929/238‑1 (MQ=255)

                                                                                                                                                                                                                         tCTACCGGGATTTCTGACACCTCTTACAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAAC‑AGGCACAGTACAACTTTGTCGGT‑GCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTcaa                       <  1:371287/239‑1 (MQ=255)

                                                                                                                                                                                                                          cttccGGTATTTCTGACACCGCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCGCGCTGCCGATTTAGCAGGGCGGAATGGTTAACTCGCAGGTGAAAC‑AGGCACAGTACAACTTTGTCGGT‑GCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTcaac                      <  1:1078502/236‑1 (MQ=255)

                                                                                                                                                                                                                                    ttCTGACCCCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAAC‑AGGCACAGTACAACTTTGTCGGT‑GCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATg            <  1:326802/239‑1 (MQ=255)

                                                                                                                                                                                                                                      ccgACACCTCTTATAGGGGTTCGAAAGCCCGTGGGGCCGGTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAAAAAAGGTGGCCT‑GAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACGCGCAGGTGAAAC‑AGGCACAGTACAACTTTGTCGGT‑GCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCa          <  1:51496/237‑1 (MQ=255)

                                                                                                                                                                                                                                       tGACCCCTCTTAAAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAAC‑AGGCACAGTACAACTTTGTCGGT‑GCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCAt         <  1:49301/239‑1 (MQ=255)

                                                                                                                                                                                                                                           ctctctTAAAGGGGTTAGAAAACCTGGGGTGCCGCTGGTCCCCGGTATGACGATAGC‑AATATGGGCCAGAAAAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAAC‑AGGCACAGTACAACTTTGTCGGT‑GCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTAt     <  2:342184/237‑1 (MQ=255)

                                                                                                                                                                                                                                              tctTTTAGGGGTTTGAAAGCCCGGGGTGCCGCTGGTACCCAGTATGGCGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAAC‑AGGCACAGTACAACTTTGTCGGT‑GCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAg  <  2:730207/239‑1 (MQ=255)

CTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGA‑CCT‑GGCGCG‑CGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTG‑GATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGC‑AATATGGGCCAGAACAAAGTTGGCCT‑GAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAAC‑AGGCACAGTACAACTTTGTCGGT‑GCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAG  >  NC_000913/3178722‑3179194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`