BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,199,933	A→G	12.2%	I344T (ATC→ACC)	ygiF ←	inorganic triphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,199,933	0	A	G	12.2%	233.4 / 2.3	108	I344T (ATC→ACC)	ygiF	inorganic triphosphatase
Reads supporting (aligned to +/- strand): ref base A (26/65); new base G (14/2); total (41/67)
Fisher's exact test for biased strand distribution p-value = 1.25e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

TTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCAT  >  NC_000913/3199702‑3200168

tttGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGGAAAAGGGGTATCGGc                                                                                                                                                                                                                                              >  1:620929/1‑239 (MQ=255)

tttGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAAAAGGAAATCGGc                                                                                                                                                                                                                                              >  1:971913/1‑239 (MQ=255)

tttGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCAGGGCGGGGAAAATGGAGATCGGc                                                                                                                                                                                                                                              >  1:112327/1‑239 (MQ=255)

 ttGCCTAATTACGGAAATGTTCATTTTAGATGCTTTCCCCGGTCGAAATAGCGTGATGCATCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCa                                                                                                                                                                                                                                             <  1:470383/239‑1 (MQ=255)

 tggcttatttcgggAAAGTTTCATTTTGGATGCGTCCCCCGGCCAAAAAAGGGGCAGCCAGCCCCTGCCAGTTCTCCAGCAAGGCTGGCGCGCCGACAGGATCATAGTACCCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCa                                                                                                                                                                                                                                             <  2:759569/227‑1 (MQ=255)

  tGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCACCTGGCCACGGTAGCGATGGCCTAACGGCGGGCAGAAAACCATTTTCCATTCAGCGGCAAGGCGGGGAAAAGGGaataaggcaa                                                                                                                                                                                                                                            >  2:226465/1‑230 (MQ=255)

          tACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGTCGGGAAAGATGGGAAGTGGCAAAGC‑Ggttt                                                                                                                                                                                                                                   >  1:900339/1‑238 (MQ=255)

                    ttataTTTCGTTTCTTTGCCCGGCCGTAAGAGCGTGATGGCCCCCGTGCCTGTTCTCCAGCCGGGCTGGCGGCACGACAGGTGAATAGTAACCCGACAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGc                                                                                                                                                                                                                       <  2:53420/235‑1 (MQ=255)

                                           tCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTTCCCTG‑CGCTTTGGCa                                                                                                                                                                                              >  1:366595/1‑239 (MQ=255)

                                                     gTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTAAGCGGCATGGCGGGCAAGATGGCGATCGGCAAAAC‑GTTT‑GAAACAGT‑ACCCGA‑TTTTGCCCCG‑GGCTATGGAATCTAAAAATg                                                                                                                                                                                    >  1:560899/1‑239 (MQ=255)

                                                         tGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCCTGGCGGGGCAGAATGAGAACCGCAAAGG‑GTTT‑GAAGGAGG‑GGCAGA‑TTTTGCCCCGCCGCTTTGCCACCAAAAAAAgggg                                                                                                                                                                                 >  2:375651/1‑237 (MQ=255)

                                                         tGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGGCAACAGGGTATACGGAAAGCGGTTT‑GGAGAAG‑‑CGCTGATTTTTGCCCTG‑‑GCCTTTGCCTCTAAAAAAgggag                                                                                                                                                                                >  2:1062275/1‑236 (MQ=255)

                                                         tGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGGTATTGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATTTAAAAAAGGCt                                                                                                                                                                                 >  1:568067/1‑238 (MQ=255)

                                                            aGCCCCGGCCAGTTCTCCAGCCAGGCTGGCGCGAAGACAGGATCATAGGACCCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑cc                                                                                                                                                                             <  1:1080776/238‑1 (MQ=255)

                                                                 cttcgcgttcctcagtagagCTTGGGGGACGACGGGAGTATAGTACCCCGCCAAGAAAAGGATTGAGGCAATAACGCGCGTAAGGCTTGGCAAGTGGGCCCGGGAGCCATCGCCTAACGGCTGGCGGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCAggctg                                                                                                                                                                       <  2:560899/220‑2 (MQ=255)

                                                                   gCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGCAAACGCTTTTCAGTTCAGCGGCATGGCGGGCAAAATGGGTATTCGCAACAG‑GCTT‑GAAGGGAC‑CGCCTA‑TTTTTCCCTT‑GGCTTTTGCATATaaaaaaaggctccgaatgttt                                                                                                                                                                      >  2:485849/1‑223 (MQ=255)

                                                                   gCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTTCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCCtgctttg                                                                                                                                                                     >  1:54396/1‑239 (MQ=255)

                                                                          ctcCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGGAAGATGGGTATCGGCAAAAG‑GTTT‑GAAGGGGT‑CGCGGA‑GTTTGACCTG‑GGCTTTGGGATTTAAAAAAGGGTG‑CCATGCTTTGCTTacca                                                                                                                                                              >  1:465652/1‑239 (MQ=255)

                                                                                  agGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAAAAGGGTATAGGCCAAGC‑GTTT‑GTAGGAGT‑CGCTGG‑TTTTGCCCTG‑CGCCTTGGGATCTAAAAATGGGGG‑CCAcgcttttttttcaaacacattg                                                                                                                                                      >  2:388644/1‑223 (MQ=255)

                                                                                          gcccggagagggccaaaggagccccccAGCAACGGTTTTGGCTCAATATCCCGCGTCACGCGTGGCAACTGGTCCCGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAATG‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCAGCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACg                                                                                                                                             <  2:112327/217‑1 (MQ=255)

                                                                                                       catagacccccGCCAGCAACAGTATTGAGTCAAAATCCCGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGGCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCAtt                                                                                                                               <  2:54396/231‑1 (MQ=255)

                                                                                                         tAGTAACCCGGCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGc                                                                                                                             <  1:706439/239‑1 (MQ=255)

                                                                                                         tAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAAC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATTGCTG‑CCCTGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCCGCTTCGCCCTTg                                                                                                                              >  1:575106/1‑238 (MQ=255)

                                                                                                          aGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATAGGCAAAGC‑GGTT‑TAAGGGGT‑CGCTGA‑TTTTTCCCTG‑CGGTTTGGCATTTAAAAATGGCGG‑CCCTGCCTTGCTTTCCAA‑CCATTCGGGCA‑ACGCCCGCtttccccttt                                                                                                                              >  1:270279/1‑229 (MQ=255)

                                                                                                           gTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑TGCTGA‑TTTTGCCCTG‑CGCCTTGGCATCTAAAAAATGCTG‑CCATGCTTTGCTTCCCAA‑CCATTCGGTCA‑AAGCCAGCTTCGCCATTTCCg                                                                                                                           >  1:588403/1‑239 (MQ=255)

                                                                                                            taccccGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGt                                                                                                                          <  1:938212/236‑1 (MQ=255)

                                                                                                            taccccGCCAGCAACAGGTTTGAGTCAATATCCCGCGTCAGGCGTGGCAACTGGTGCCGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGt                                                                                                                          <  2:197050/236‑1 (MQ=255)

                                                                                                              cccccccACCACCAGTTTTGGGTCAAATTCACGCGGCAGGCGGGGCAAATGGTGACGGTAGAGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGttt                                                                                                                        <  2:1097805/238‑1 (MQ=255)

                                                                                                              aCCCGCCAGTAACAGTTTTGAGTCAATTGCACGCGTCAGGCGGGGCAACTGGTCACGGTAGCGAACGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGttt                                                                                                                        <  1:617501/239‑1 (MQ=255)

                                                                                                               cccGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTc                                                                                                                       >  1:915414/1‑239 (MQ=255)

                                                                                                               cccGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGGCA‑ACGCCAGCTTCGCCCTTGCCGTTTc                                                                                                                       >  1:197050/1‑239 (MQ=255)

                                                                                                               cccGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGAAGGGAACACGCGAAGC‑GTTG‑TAAAGAGTCCGCGGG‑TTTT‑CCCCG‑CGGTTTGGTGTCTGAAAATAGGTGCCCAGGCTTGGTTTA‑CAA‑ACATTTCGTCACACCCCGGCTCCGCCAtgccggttt                                                                                                                        >  2:465010/1‑231 (MQ=255)

                                                                                                                cccccagacacatttttGTGTCAATACCCCGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCg                                                                                                                      <  2:588403/224‑1 (MQ=255)

                                                                                                                 cGCCAGCAACAGTATTGAGTCAATGTGCCGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATAGCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCgg                                                                                                                     <  1:224471/239‑1 (MQ=255)

                                                                                                                 cGCCAGCAACAGTATTGAGCCAATAGCCCGCGTCAGGCGTGGCAACTGGTCACGTGAGCGATCGCCTAACGGCTAGCAGAAAACGCTTTACAGTTCAGCGGCATGGCGGGAAAGGTGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTTCCCTT‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCgg                                                                                                                     <  1:75718/239‑1 (MQ=255)

                                                                                                                  gCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGGGATCGGCAAAAC‑GTTT‑GAAAGAGT‑CGCGGG‑TTTTGCCCTG‑CGCTTTTGCATCTAAAAATGGCTG‑CCAGGCTTTTGTTACCAA‑CACATCGGGTG‑ACGCCACCTCCGCCCTTGCCGGTTgggg                                                                                                                    >  2:916542/1‑238 (MQ=255)

                                                                                                                   ccAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCCGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAAC‑GTTT‑TAAGGAGC‑CGCTGG‑TTTTGCCCTG‑CGCCTTGGCATCTCAAAAAGGCGG‑CCCTGCTTTTCTTTCCAC‑CCATTCGTTCA‑ACGCCAGCTTCGCCCATTCCGTTTCggga                                                                                                                   >  2:831207/1‑237 (MQ=255)

                                                                                                                             tATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCa                                                                                                                                                                                              <  1:12420/157‑1 (MQ=255)

                                                                                                                              ttggAGTCAATATCCCGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGAcc                                                                                                          <  1:443681/234‑1 (MQ=255)

                                                                                                                                  aGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGGTATCGGCCAAAC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTC‑CGCTTTGGCCTCTAAAAATGGCTG‑CCATGCTTTTCTTCCCCA‑CCACTCGGTCA‑CACCCACCTTCGCCCCTTCCCTTTCGGTAGAGGAAAccgccgc                                                                                                     >  2:443681/1‑237 (MQ=255)

                                                                                                                                    tCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGGAAGATAGGTATCGGCAAAAC‑GTTT‑GAAGAGGT‑CGCTGG‑TTTTTCCCTT‑CGATTTGGTGTCTAAAAATGGCTG‑CCCTGATTTGCTTACCAA‑CCACTCGGTCA‑ACCCCAGCCACGCCCCTTCCGTTTCGGGTGAGAAGAcacccctccc                                                                                                  >  2:182994/1‑230 (MQ=255)

                                                                                                                                    tCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGAAGACCGCCGTAAc                                                                                                  >  1:937238/1‑239 (MQ=255)

                                                                                                                                       atatCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGGAATCGGCAAAAC‑GTTT‑TAAGGGGC‑GGCGGA‑TTTTTCCCTG‑GGCTTTTGGCTCTAAAAAAGGCGG‑GCAGTCTCTGTTTTCCAA‑CCATTTGGCCC‑ACCCCAACCACGCCCTATCCGTTTCTgtggagataacccccccgccgcc                                                                                               >  2:365827/1‑222 (MQ=255)

                                                                                                                                       atatCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CCCTTTGGCATCTAAAAAAGGCTG‑CCATGCTTTGCTTACCAC‑CCATTCGGGCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGGAAAGGAGACCGCCGTCACGGc                                                                                               >  1:1033943/1‑239 (MQ=255)

                                                                                                                                        cttccccggtctgggttgggcaCTGGTCACGGGGGCGGTCGCCGAACGGGTGGCAAGAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGG‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCa                                                                                              <  2:894356/218‑1 (MQ=255)

                                                                                                                                           gggggggcgggggTGGAATCTTGTCACGGGAGCAGTCGCCTAACGGCTGGCAGAAGACGCTTTTCAGTTCGGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGa                                                                                            <  2:436145/226‑1 (MQ=255)

                                                                                                                                           cACGGGTCAGGCGTGGCAACTGGGCACGGTAGGGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGac                                                                                           <  1:960273/239‑1 (MQ=255)

                                                                                                                                            aCGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑TAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCAAGCTTTCCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCCTTGCCGTTTCCGTAGAGTAGACCGCCGTCACGGCAGac                                                                                           >  1:524635/1‑238 (MQ=255)

                                                                                                                                                ttagggCGTGGCAATTGGTCAGGGTAGCGATCGCCTAACGGTTGGAAGAAAACGCTTTTCGGTTCAGCGGCATGGGGGGAAAGAGGGATACCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCGG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCg                                                                                       <  2:465652/234‑1 (MQ=255)

                                                                                                                                                  cAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGGTGGGTATCGGCAAAGC‑GTTT‑GAATGAAT‑CGGGGG‑TTTTGCCCCG‑CGCTGTGGACTCTAAAAAAGGCGG‑CCACGCCTTCGTTTCCAACCCATCGGGGCA‑AC‑CCAACATCGCCACTGCCGTTTCGGAGAAGGAGAACCCCGTCCCGGCAGacacaccaa                                                                                    >  2:770554/1‑234 (MQ=255)

                                                                                                                                                  cAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCa                                                                                     <  1:182994/238‑1 (MQ=255)

                                                                                                                                                  cAGGCGTGGCAACTGGGCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCa                                                                                     <  1:153986/238‑1 (MQ=255)

                                                                                                                                                  agggggttcgaaCGGGTCCCGGTAGCTGTCGCCGAACGGGTGGCAGAAAACGCTTTTCAGTTCAGGGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCa                                                                                     <  2:354702/228‑1 (MQ=255)

                                                                                                                                                       gTGGAAACTGGTCACGGTTGCGATCGCCTAACGGGTGGCAGAAAAGGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGc                                                                                <  1:739443/238‑1 (MQ=255)

                                                                                                                                                        tGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTTGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGGGTAGACCGCCGTCCCGGCCGACACCGCAGAAGccg                                                                              >  1:436145/1‑237 (MQ=255)

                                                                                                                                                          gCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAAACCGCCGTCACGGCAGAAACCCCCAAAGCaaag                                                                            >  1:769605/1‑237 (MQ=255)

                                                                                                                                                          gCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGGAGACCGCCGTCACGGCAGCAACCCCCGAACCAATg                                                                            >  1:1018220/1‑239 (MQ=255)

                                                                                                                                                             aCTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTc                                                                         <  1:275980/239‑1 (MQ=255)

                                                                                                                                                             aCTGGTCACGGGAGCGATCGCCTAACGGTTGGCAGAAAACGGTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTc                                                                         <  1:619121/239‑1 (MQ=255)

                                                                                                                                                               tggtgcgggtggggATCGCCTAAAGGCTGGCAGAAAACGCTTTTCAGGTCAGCGGCATGGCGGGAAAGGTGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGc                                                                       <  2:178523/226‑1 (MQ=255)

                                                                                                                                                               tGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGc                                                                       <  1:1062275/239‑1 (MQ=255)

                                                                                                                                                                ggTCCCGGTGGCAGTAGCCTAACGGGTGGCAGAAAACGCTTTTCAGTTCGGGGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGcc                                                                      <  1:251014/239‑1 (MQ=255)

                                                                                                                                                                 gcgggggggtgggtCGCCAAAGGGGTGGGAAAAAACGCTTTTGAGTTAAGCGGCTTGGCGGGAAAGAGGGATTTCGGCAAGGC‑GTTT‑GAAGGAGT‑CGCGGA‑TTTGGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGcc                                                                      <  2:786345/225‑1 (MQ=255)

                                                                                                                                                                 gTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGt                                                                                                                          <  1:375792/186‑1 (MQ=255)

                                                                                                                                                                  tCACGGTAGCGATCGGCTAAAGGCTGGCAGAAAACGCTTTTCAGTTCAGGGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCt                                                                     <  1:303259/238‑1 (MQ=255)

                                                                                                                                                                    aCGGGAGCGATCCCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGc                                                                  <  1:375651/239‑1 (MQ=255)

                                                                                                                                                                     cGGTGGCGATTCCCTAACGGATGGCAGAAAACCGTTTGCATTTCGGCGGCATGGGTGGAAAGATGGGTTTCGGC‑AAGT‑GTTTGGAAGGGTT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAAGGGCTG‑CCATGCTTTGCTTACAAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCa                                                                 <  2:900339/239‑1 (MQ=255)

                                                                                                                                                                             aTCGCCTAACGGCTGGCAAAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTc                                                          <  1:243147/238‑1 (MQ=255)

                                                                                                                                                                                ggcTAACGGCTGGCAGAAAACGCTTTGCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTcagca                                                      <  1:71326/237‑1 (MQ=255)

                                                                                                                                                                                gCCTAACGTCGGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATAGCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTcagca                                                      <  1:144882/239‑1 (MQ=255)

                                                                                                                                                                                gCCTAACGGCTTGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTcagca                                                      <  1:118308/239‑1 (MQ=255)

                                                                                                                                                                                gCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCa                                                                                                                                                                                                                                             >  1:761282/1‑64 (MQ=255)

                                                                                                                                                                                gCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCa                                                                                                                                                                                                                                             <  2:761282/64‑1 (MQ=255)

                                                                                                                                                                                gCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATTCAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGGAGGGTAGAACGCCGTCCCGGCCGACACCCACGAAGCCAAGGTCGCCCCGCAATGAGTctt                                                        >  1:786345/1‑235 (MQ=255)

                                                                                                                                                                                  ccaaCGGCTGGAAGAAAACGGTTTTTCGGTCAGGGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGa                                                    <  2:915414/237‑1 (MQ=255)

                                                                                                                                                                                  cTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGa                                                    <  1:5475/239‑1 (MQ=255)

                                                                                                                                                                                    aaCGGGTGGCAAAAAACGTTTTTCAGTTCAGCGGCGTGGGGGGAAAGGTGGATATCGGAAAAGC‑GTTT‑GAAGGAGT‑CGCTTA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATc                                                  <  2:769605/239‑1 (MQ=255)

                                                                                                                                                                                       ggCTGGCAAAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACg                                               <  1:916542/239‑1 (MQ=255)

                                                                                                                                                                                                aaaaCGCTTTTCCGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAagtgagt                                      <  1:936467/239‑1 (MQ=255)

                                                                                                                                                                                                aaaaCGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAAAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGGCCGCTCGCATTTAGGCAGCAGATCACCTAagggagt                                      >  1:78349/1‑239 (MQ=255)

                                                                                                                                                                                                 aaacttttttCTGTTCAGGGGCATGGGGGGAAAGAGGGATATCGGCAAAGC‑GTTT‑GAAGGAGC‑CGCTGA‑TTTTGCCCTG‑CGTTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAagtgagtg                                     <  2:937238/233‑1 (MQ=255)

                                                                                                                                                                                                 aaaccattttCAGTTCAGGGGCATGGCGGGAAAGATGGATAACGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTTA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAagtgagtg                                     <  1:1006865/233‑1 (MQ=255)

                                                                                                                                                                                                 aaaCGCTTTTCAGTTGAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAagtgagtg                                     <  1:228562/239‑1 (MQ=255)

                                                                                                                                                                                                   aCGTTTTTCGGTTCAGGGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCt                                   <  1:442547/239‑1 (MQ=255)

                                                                                                                                                                                                            gtttgggCGGCTTGGGGGGAAAGAGGGATCTCGGCAAAGC‑GTTT‑GAAGGATT‑CGCTGA‑CTTTCCCCTG‑CGCTTTGGCATCGAAAAATGGGTG‑CCTGGCTTTGCTTGCCAA‑CCATTCGGTCA‑ACGCCAGCCTCGTCATTGCCGGTTCGGTAGAATAGGCCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGATTTAcg                          <  2:564369/233‑1 (MQ=255)

                                                                                                                                                                                                            agttccgGGGCGTGGGGGGAAATGTGGATTTCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTTA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTAcg                          <  2:366595/239‑1 (MQ=255)

                                                                                                                                                                                                            agttcagGGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGTGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTAcg                          <  1:124646/239‑1 (MQ=255)

                                                                                                                                                                                                            agttcagCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTAcg                          <  1:169809/239‑1 (MQ=255)

                                                                                                                                                                                                               tcagCGGCCTGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTAcg                          <  1:306666/236‑1 (MQ=255)

                                                                                                                                                                                                               tcagCGGCATGGCGGGAAAGTGGGATACCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTAcg                          <  1:4402/236‑1 (MQ=255)

                                                                                                                                                                                                               tcagCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATTTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCCTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTAcg                          <  1:72031/236‑1 (MQ=255)

                                                                                                                                                                                                                 cggtggaTGGGGGGAAAGAGGGGTATCGGCAAAGG‑TTTT‑GAAGGGGT‑CTCTTA‑TTTGCCCCGG‑CCCTTTGGCATCTAAAAAGGCGTG‑CCATGCTTTGCTTACCAA‑CCATCCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGca                     <  2:78349/233‑1 (MQ=255)

                                                                                                                                                                                                                     gCATGGCGGGAAAGGTGGATATCGGCAAAGG‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACaa                  <  1:485849/238‑1 (MQ=255)

                                                                                                                                                                                                                      cATGGCGGTAAAGGTGGATATCGGCAAAGG‑GTTT‑GAAGGTGG‑CGCTGA‑TTTTGCCCTG‑CGTTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTa                            <  1:514912/227‑1 (MQ=255)

                                                                                                                                                                                                                        tGGCGGGAAAGATGGATAACGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTTGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGTGGCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATac               >  1:812095/1‑238 (MQ=255)

                                                                                                                                                                                                                           cGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTcagca                                                      <  1:180685/196‑1 (MQ=255)

                                                                                                                                                                                                                               aaagtgggATTTCGGCAAGGC‑GTTT‑GAAGGATG‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTCCCAA‑CCATTCGGTCA‑ACGCCAGCTGCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGaa        <  2:812095/232‑1 (MQ=255)

                                                                                                                                                                                                                                     ggATTTCGGCAAAGC‑GTTT‑GAAGGGGC‑CGCTGA‑TTTTGCCCTG‑CGCGTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCAt  <  1:457973/238‑1 (MQ=255)

                                                                                                                                                                                                                                     ggATATCGGCAAAGG‑TTTT‑GAAGGAGG‑CGATGT‑TTTTCACCGT‑GTCTTGGGCATGTAAAAATGGCGT‑CCATGCTTTTCTTACCAA‑CCCTTCGGTCC‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTTGACCGCCGTCACGGCAGACTCCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCGCGCTTTACGCGGCACAATACCACCGAACAGCAt  <  2:273147/238‑1 (MQ=255)

                                                                                                                                                                                                                                     ggATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGGGCTCGCTTTACGCGGCACAATACCACCGAACAGCAt  >  1:7296/1‑238 (MQ=255)

TTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGC‑GTTT‑GAAGGAGT‑CGCTGA‑TTTTGCCCTG‑CGCTTTGGCATCTAAAAATGGCTG‑CCATGCTTTGCTTACCAA‑CCATTCGGTCA‑ACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCAT  >  NC_000913/3199702‑3200168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`