Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,206,444 | C→A | 100% | intergenic (‑190/‑17) | ttdR ← / → ttdA | transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,206,444 | 0 | C | A | 53.6% | 25.0 / 50.3 | 33 | intergenic (‑190/‑17) | ttdR/ttdA | transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit |
Reads supporting (aligned to +/- strand): ref base C (1/14); new base A (2/16); total (3/30) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.83e-05 | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch. |
CCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTA‑TTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTT‑A‑GTGGTAGTTTGTGGCGGTG‑AA‑TTTTTC‑C‑AGACAAATACAAAAAC‑TGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACA‑GGCAGTGAATAAGTT‑GACAGAGATTGTCGCTAACTTTACCGCCATGATTTC‑TACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCC > NC_000913/3206212‑3206666 | ccAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTA‑TTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTT‑A‑GTGGTAGTTTGTGGCGGTG‑AA‑TTTTTC‑C‑AGACAAATACaaaaa < 1:655954/238‑1 (MQ=255) tttaccgCAAGTTTTTGGCTAAAGGCCGGCTATTTCGGCA‑AGTTTATTTTCCCTAGGTGGTATAAGCGTAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGTAATATTCATTCTTAGTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTT‑A‑GTGGTAGTTTGTGGCGGTG‑AA‑TTTTTC‑C‑AGACAAATACAAAAAC‑Tgg < 2:891046/233‑1 (MQ=255) tACCTGCAAGTCTTTGGCTAAAGGCCAGCTA‑TTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATTAAACCACATTTTT‑A‑GTGGTAGTTTTTGGCGGGG‑CA‑TATTTT‑C‑CGAaaaaaaaaaaaaaagga > 2:611909/1‑234 (MQ=255) tACCTGCAAGTCTTTGGCTAAAGGCCAGCTA‑TTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTT‑A‑GTGGTAGTTTGTGGCGGTG‑AATTTTTTC‑C‑TAAAAAAAAAAAAAAC‑Tgg > 1:623578/1‑239 (MQ=255) tACCTGCAAGTCTTTGGCTAAAGGCCAGCTA‑TTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTT‑A‑GTGGTAGTTTGTGGCGGGGAAA‑TTTTTC‑T‑TAacaaaaacaaaacagta > 1:568357/1‑234 (MQ=255) cAAGTCTTTGGCTAAAGGCCAGCTA‑TTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTT‑A‑TTGGTAGTTTGTGGCGGGGAAA‑TTTTTA‑C‑TAACAaaaaaaaaaaaagagatgtt > 2:655954/1‑229 (MQ=255) tAAAGGCCCTTTT‑TTCAACATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTTTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTT‑A‑GTGGTAGTTTGTGGCGGTG‑AA‑TTTTTC‑C‑AGACAAATACAAAAAC‑TGGAGTTGCCATGATGAGCGa < 2:461500/238‑1 (MQ=255) tAAAGGCCAGCTA‑TTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTT‑A‑GTGGTAGTTTGTGGCGGTG‑AA‑TTTTTC‑C‑AGACAAATACAAAAAC‑TGGAGTTGCCATGATGAGCGa < 1:386474/238‑1 (MQ=255) tAAAGGCCAGCTA‑TTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCACAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTT‑A‑GTGGTAGTTTGTGGCGGTG‑AA‑TTTTTC‑C‑AGACAAATACAAAAAC‑TGGAGTTGCCATGATGAGCGa < 1:807905/238‑1 (MQ=255) ttCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTT‑A‑GTGGTAGTTTGTGGCGGTG‑AA‑TTTTTC‑C‑AGACAAATACAAAAAC‑TGGAGTTGCCATGATGAGCGa < 1:1094873/225‑1 (MQ=255) ttCAGCATCGTTAAGTAGCCGCAATTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTT‑A‑GTGGTAGTTTGTGGCGGTG‑AA‑TTTTTC‑C‑AGACAAATACAAAAAC‑TGGAGTTGCCATGATGAGCGAAAGTAATAAGCAAc < 1:157955/239‑1 (MQ=255) attttccgctgtttgtaaaaGCGATGTGTATTTCGCAAAACATTGCCATTCCCGGCTCTGGCGCAATATTCATGCTTATTTTAGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTT‑A‑GTGGTAGTTTGTGGCGGTG‑AA‑TGTTGC‑C‑AGACAAATACAAAAAC‑TGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACA‑GGCAGTGAATaa < 2:82882/221‑1 (MQ=255) aTCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTT‑A‑GTGGTAGTTTGTGGCGGTG‑AA‑TTTTTC‑C‑AGACAAATACa < 1:723557/183‑1 (MQ=255) gCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTT‑A‑GTGGTAGTTTGTGGCGGTG‑AA‑TTTTTC‑C‑AGACAAATACAAAAAC‑TGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACA‑GGCAGTGAATAAGTT‑GACa < 1:776637/239‑1 (MQ=255) acgcaattttttttCGAAAAACATTTCCCTTCCCTGCTCTGGCGCAATTTTCATTCTTATTTTCGCGTTTATTCACGAACTGTTAATTACATATTCAGGGTGGAAAAAAATATAAAACCACATTTTT‑A‑GTGGTAGTTGGTGGCGGTG‑AA‑TTTTTC‑C‑AGACAAATACAAAAAC‑TGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACA‑GGCAGTGAATAAGTT‑GACAGAGATTGTCGCt < 2:266195/228‑1 (MQ=255) ttttttcaaaaaaaaTTGCCTTTCCCTGCTTTGGCACAATTTTCTTTCTTATTTTCGCTTTGATTCACGATGTGTTAATTACATTTTCACGGTGGAAAAAAATAAAAAACCACATTTTA‑A‑GTGGTAGTTTGTGGGGGTA‑AT‑TTTTAC‑C‑AGAAAAATAAAAAACC‑TGGTGTGGCCATGATGAGCGAAATTAATAAGCAACA‑GGCAGTGAATAAGTT‑GACAGAGATGGCCGCTAACTTTAc < 2:658940/230‑1 (MQ=255) ctgggcaaaaTATCTATTTTTATTTCCGGGTTTATTAACGAACGTTTAATTACATATTCACGGTGGAAAAAAATATAAAACCATATTTTT‑A‑GTGGTAGTTTGGGGCGGTA‑AA‑TTTTTC‑A‑AAACAAATAAAAAAAC‑GGAGGTTGCCTTGATGAGCAAAAGTAATAAGCAACA‑GGCAGTAAATAAGTT‑GACAGAGATTGTCGCTAACTTTACCGCCATGATTTC‑TACCCGAATGCCtgatg < 1:427985/231‑1 (MQ=255) cctattttttttttttttttCGGTTTTATTCACGAATTTTTAATGTCATATCCGGGGGGGAAAAAAATAAAAAACCATTTTTTT‑A‑GTGTTTGTTTGGGGGGTTG‑AT‑TTTTTC‑C‑AGAAAAATAAAAAAAC‑GGGATTTGCCATGATGAGCAAAAGAAATAAGCAACG‑GGCAGTAAATAAGTT‑AACAGATATTGTCGCTAACTTTACCGCCATGATTTC‑TACCCGAATGCCTGATGACgtg < 2:1101367/222‑1 (MQ=255) ttttttttttttttttCGGGTTGATTCACAAATTTTAATTTACATATTCCCGGAGGAAAAAAATAAAAACCCACATTTTT‑T‑GTGGTGGTTGGTGGGGGT‑‑AA‑TTTTTA‑C‑AAAAAAAACAAAAAACGGGGTGTTGCGATGATGAGAGAAAGAAATAAGCAACA‑GGAATTAAATAAGTT‑GACAGAGTTTGTCGTTATTTTTACCGCCATGATTTC‑TACCCGAATGCCTGATGACGTGGTGGa < 2:595982/227‑1 (MQ=255) tatTCATTCTTATTTCCGCTTTGATTCACGAACTGTTAATTACATATGCACGGTGGAAAAAAATAAAAAACCAAATTTTT‑A‑GTGGTAGTTTGGGGGGGTA‑AA‑TTTTTC‑C‑AGACAAATAAAAAAAC‑TGGAGTTGCCATGATGAGCGAAAGAAATAAGAAACA‑GGCAGTAAATAAGTG‑GACAGAGTTTGTCGCTAACTTTACCGCCATGATTTC‑TACCCGAATGCCTGATGACGTGGTGGa < 1:780561/239‑1 (MQ=255) ctttcTTATTTTCGGGTTAATTCACGAATTTTTAATTAATTTTTCAGGGTGGAAAAAAATATAAAACCA‑ATTTTT‑ATGTGGTAGTTTGGGGCGGTG‑AA‑TTTTTC‑C‑AGAAAAATAAAAAAAC‑GGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACA‑GGCAGTGAATAAGTT‑GACAGAGATTGTCGCTAACTTTACCGCCATGATTTC‑TACCCGAATGCCTGATGACGTGGTGGATaaa < 1:538339/237‑1 (MQ=255) ttttttcttgtttttacccaatgtGTTAATTACTTAGTGAGGGGTACAAAAAATAAAAAACCGAGTTTTT‑G‑GGGGTAGTTTGTGGAGGTG‑AA‑TTTTTC‑C‑ATAAAAATAAAAACAT‑GGGAGTTGCCATGATGAGGGAAAGAAATAAGCAACG‑GGAAGTGAAGAAGTA‑GAAAGGGATGGCTGATAATTTTACCGCCATGATTTC‑TACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa < 2:865749/216‑1 (MQ=255) aatttgcggtttGATTCACGGTCGGTAAATTACATATGCGGGGTGGAAAAAAAGAAAAACCCAAATTTTA‑A‑GGGGTAGTTTGGGGCGGTA‑AA‑TTTTTC‑C‑AAAAAAAAAAAAAAAC‑TGGAGTGGCGATGATGAGGAAAAGTAATAAGAAACA‑GGAGGTAAATAAGTT‑GAAAGAGATTGTCGCTAACTTTACCGCCATGATTTC‑TACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa < 1:915225/229‑1 (MQ=255) ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTT‑A‑GTGGTAGTTTGTGGCGGTG‑AA‑TTTTTC‑C‑AGACAAATACAAAAAC‑TGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACA‑GGCAGTGAATAAGTT‑GACAGAGATTGTCg < 1:567576/155‑1 (MQ=255) ttttCCCGGTGGAAAAAAAAATAAACCCCAATTTTT‑A‑GGTGTAGTTTGTGGCGGTA‑AA‑TTTTCC‑C‑AAAAAAATAAAAAAAT‑TGGAGTTCCCATGATGAGCGAAAGTAATAAGCAACA‑GGAAGTGAATAAGTT‑GACAGAGTTTGTCGTTACTTTTACCGCCATGATTTC‑TACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa < 1:1052581/237‑1 (MQ=255) atTCACGGTGGCAAAAAATATAAAACCACATTTTT‑A‑GTGGTAGTTTGTGGCGGTG‑GA‑TTTTTT‑C‑AAAAAAATAAAAAAAC‑TAGAGGTGTCATGATTAGGGAAAAGAATAAACAACA‑AGCAGTGAATAAGTT‑GACAGAGAATTTTGCTAACATTTCCGCCACGATTTCTTACCCAAATGCCT‑CTGACGGGGTGGGTAAAATAAAAAAACTTAAGGATGCCGCAAAGCCGGCGATggggga > 1:333808/1‑237 (MQ=255) ttaagggggaaaaaaaaTAAAAAACCAAATTTTT‑G‑GGGGAAGTTGGGGGGGGTG‑AA‑TTTTTC‑C‑AGACAAATAAAAAAAG‑TGGAGTTGCCAGGATGAGCAAAAGTAATAAGCAACA‑GGCAGTGAATAAGTT‑GAAAGAGTTTGTCGCAAATTTTACCGCCATGATTTC‑TACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGGCGATGGGGa < 1:325485/226‑1 (MQ=255) gtCACGGTGAGAAAAAATAAAAACACAATTGTTG‑T‑GTGGTGTTTGGTGGCGTAA‑AA‑TTTTCC‑C‑AAACAAACACAAAATC‑TGGAGTTGCCATGGTAGGCAAAAGAAATAAGAAACC‑GGAAGTGAAAACGTG‑GACATGGATGGTCCCAATCTTTACCGCCATGATTTC‑TACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa < 1:790916/236‑1 (MQ=255) ttggcaaaaaaaTAAAAACCCATATTTTA‑T‑GTTGTAGTTGGGGGGCGTA‑AT‑TTTTTA‑A‑ACAAAAAAAAAAAATC‑TGGGGTTCCAATGTTGGGGGAAAGTAATAAGCAACA‑GGGTGTAAATAAGTA‑GACAGAGTTTGGCGCAAAGTTTACCGCCATGATTTC‑TACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt < 2:1077924/233‑1 (MQ=255) gTGGAAAAAAAGAT‑AAACCATATTTTTAA‑GTGGAATTTTGTGGGGGTG‑AA‑TTTTTC‑C‑AAACAAAAACAAAATC‑TGGGTTTGCCATGATGGGCAAAATAAAAAAGCAAAA‑GGCAGTAAATAGTTT‑GACATGGTTTGTCGCTACTTTTACCGCCATGATTTC‑TACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt < 1:237497/238‑1 (MQ=255) tctttttt‑A‑GGTGTAGTGTGTGGCGGT‑‑AA‑TTTTTC‑CAAAAAAAATAAAAAAAC‑GGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACG‑GGAAGTGAATAAGTT‑GAAAGAGATTGTCGTTAACTTTACCGCCATGATTTC‑TACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGacaa < 1:95589/236‑1 (MQ=255) cttttttt‑A‑GTGGGTTTTTG‑GGGGGGG‑AA‑TTTTTCAC‑AGAAAAATAAAAAATG‑GGGATTCACCGTGATGGCAAAAAGAAATAAG‑AACACGGTAGTAAATAATTT‑GAGAGTGGTGGCCGCTAACTATACCGCCATGATTTC‑TACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGacaa < 2:1039425/236‑1 (MQ=255) attttttt‑G‑GTGGTATTTTGTGGGGGAG‑AA‑TTTTAC‑C‑AAAAAAAAAAAAAAAC‑TGGGTTTGCCATGAGGAGCAAAAAAAAAAAACAACG‑GGAAGTAAATAAGTG‑AACAGATATTGTCGCTAACTTTACCGCCATGATTTC‑TACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGacaa < 2:333808/236‑1 (MQ=255) gtata‑TTTTTC‑C‑AAAAAAATAAAAAAAC‑TGGGTGTGCCTTGAGGAGTGAAGATAATAAGCAACT‑GGCAG‑GAGTATGTTGGAAGGAAGTTGCCCGAAACTTACCCTCCATGATTTC‑TACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAATGATGCCGAAACGTCGGCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATACAAAAAGCGATTGACCTGAAt < 2:486584/235‑1 (MQ=255) tttttC‑C‑CAAAAAAAAAAAAAAC‑TGGTGTTGCCATGATGAGCGAAAGAAAAAAACAACG‑GGCTGTGAATAGGTT‑GCAAGGTATGGCCGTTAACTTTACCGCCATGATTGC‑TACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcc < 1:861262/239‑1 (MQ=255) cAAATACAAAAAC‑TGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACA‑GGCAGTGAATAAGTT‑GACAGAGATTGTCGCTAACTTTACCGCCATGATTTC‑TACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTa > 1:294178/1‑181 (MQ=255) | CCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTA‑TTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTT‑A‑GTGGTAGTTTGTGGCGGTG‑AA‑TTTTTC‑C‑AGACAAATACAAAAAC‑TGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACA‑GGCAGTGAATAAGTT‑GACAGAGATTGTCGCTAACTTTACCGCCATGATTTC‑TACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCC > NC_000913/3206212‑3206666 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |