Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,206,444 C→A 100% intergenic (‑190/‑17) ttdR ← / → ttdA transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4440CA53.6% 25.0 / 50.3 33intergenic (‑190/‑17)ttdR/ttdAtranscriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (1/14);  new base A (2/16);  total (3/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.83e-05
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

CCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCC  >  NC_000913/3206212‑3206666
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                  
ccAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACaaaaa                                                                                                                                                                                                                               <  1:655954/238‑1 (MQ=255)
   tttaccgCAAGTTTTTGGCTAAAGGCCGGCTATTTCGGCAAGTTTATTTTCCCTAGGTGGTATAAGCGTAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGTAATATTCATTCTTAGTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTgg                                                                                                                                                                                                                          <  2:891046/233‑1 (MQ=255)
    tACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATTAAACCACATTTTTAGTGGTAGTTTTTGGCGGGGCATATTTTCCGAaaaaaaaaaaaaaagga                                                                                                                                                                                                                          >  2:611909/1‑234 (MQ=255)
    tACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCTAAAAAAAAAAAAAACTgg                                                                                                                                                                                                                          >  1:623578/1‑239 (MQ=255)
    tACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAAATTTTTCTTAacaaaaacaaaacagta                                                                                                                                                                                                                           >  1:568357/1‑234 (MQ=255)
          cAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTATTGGTAGTTTGTGGCGGGGAAATTTTTACTAACAaaaaaaaaaaaagagatgtt                                                                                                                                                                                                                     >  2:655954/1‑229 (MQ=255)
                      tAAAGGCCCTTTTTTCAACATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTTTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                                                                                                                        <  2:461500/238‑1 (MQ=255)
                      tAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                                                                                                                        <  1:386474/238‑1 (MQ=255)
                      tAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCACAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                                                                                                                        <  1:807905/238‑1 (MQ=255)
                                    ttCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                                                                                                                        <  1:1094873/225‑1 (MQ=255)
                                    ttCAGCATCGTTAAGTAGCCGCAATTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAAc                                                                                                                                                                                          <  1:157955/239‑1 (MQ=255)
                                                 attttccgctgtttgtaaaaGCGATGTGTATTTCGCAAAACATTGCCATTCCCGGCTCTGGCGCAATATTCATGCTTATTTTAGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATGTTGCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATaa                                                                                                                                                                            <  2:82882/221‑1 (MQ=255)
                                                    aTCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACa                                                                                                                                                                                                                                   <  1:723557/183‑1 (MQ=255)
                                                        gCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACa                                                                                                                                                                    <  1:776637/239‑1 (MQ=255)
                                                                    acgcaattttttttCGAAAAACATTTCCCTTCCCTGCTCTGGCGCAATTTTCATTCTTATTTTCGCGTTTATTCACGAACTGTTAATTACATATTCAGGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTGGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCt                                                                                                                                                        <  2:266195/228‑1 (MQ=255)
                                                                            ttttttcaaaaaaaaTTGCCTTTCCCTGCTTTGGCACAATTTTCTTTCTTATTTTCGCTTTGATTCACGATGTGTTAATTACATTTTCACGGTGGAAAAAAATAAAAAACCACATTTTAAGTGGTAGTTTGTGGGGGTAATTTTTACCAGAAAAATAAAAAACCTGGTGTGGCCATGATGAGCGAAATTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATGGCCGCTAACTTTAc                                                                                                                                                <  2:658940/230‑1 (MQ=255)
                                                                                                         ctgggcaaaaTATCTATTTTTATTTCCGGGTTTATTAACGAACGTTTAATTACATATTCACGGTGGAAAAAAATATAAAACCATATTTTTAGTGGTAGTTTGGGGCGGTAAATTTTTCAAAACAAATAAAAAAACGGAGGTTGCCTTGATGAGCAAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgatg                                                                                                                  <  1:427985/231‑1 (MQ=255)
                                                                                                               cctattttttttttttttttCGGTTTTATTCACGAATTTTTAATGTCATATCCGGGGGGGAAAAAAATAAAAAACCATTTTTTTAGTGTTTGTTTGGGGGGTTGATTTTTTCCAGAAAAATAAAAAAACGGGATTTGCCATGATGAGCAAAAGAAATAAGCAACGGGCAGTAAATAAGTTAACAGATATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg                                                                                                             <  2:1101367/222‑1 (MQ=255)
                                                                                                                   ttttttttttttttttCGGGTTGATTCACAAATTTTAATTTACATATTCCCGGAGGAAAAAAATAAAAACCCACATTTTTTGTGGTGGTTGGTGGGGGTAATTTTTACAAAAAAAACAAAAAACGGGGTGTTGCGATGATGAGAGAAAGAAATAAGCAACAGGAATTAAATAAGTTGACAGAGTTTGTCGTTATTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                        <  2:595982/227‑1 (MQ=255)
                                                                                                                   tatTCATTCTTATTTCCGCTTTGATTCACGAACTGTTAATTACATATGCACGGTGGAAAAAAATAAAAAACCAAATTTTTAGTGGTAGTTTGGGGGGGTAAATTTTTCCAGACAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGAAATAAGAAACAGGCAGTAAATAAGTGGACAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                        <  1:780561/239‑1 (MQ=255)
                                                                                                                       ctttcTTATTTTCGGGTTAATTCACGAATTTTTAATTAATTTTTCAGGGTGGAAAAAAATATAAAACCAATTTTTATGTGGTAGTTTGGGGCGGTGAATTTTTCCAGAAAAATAAAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaaa                                                                                                    <  1:538339/237‑1 (MQ=255)
                                                                                                                             ttttttcttgtttttacccaatgtGTTAATTACTTAGTGAGGGGTACAAAAAATAAAAAACCGAGTTTTTGGGGGTAGTTTGTGGAGGTGAATTTTTCCATAAAAATAAAAACATGGGAGTTGCCATGATGAGGGAAAGAAATAAGCAACGGGAAGTGAAGAAGTAGAAAGGGATGGCTGATAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                              <  2:865749/216‑1 (MQ=255)
                                                                                                                             aatttgcggtttGATTCACGGTCGGTAAATTACATATGCGGGGTGGAAAAAAAGAAAAACCCAAATTTTAAGGGGTAGTTTGGGGCGGTAAATTTTTCCAAAAAAAAAAAAAAACTGGAGTGGCGATGATGAGGAAAAGTAATAAGAAACAGGAGGTAAATAAGTTGAAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                              <  1:915225/229‑1 (MQ=255)
                                                                                                                                                      ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCg                                                                                                                                                          <  1:567576/155‑1 (MQ=255)
                                                                                                                                                               ttttCCCGGTGGAAAAAAAAATAAACCCCAATTTTTAGGTGTAGTTTGTGGCGGTAAATTTTCCCAAAAAAATAAAAAAATTGGAGTTCCCATGATGAGCGAAAGTAATAAGCAACAGGAAGTGAATAAGTTGACAGAGTTTGTCGTTACTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                            <  1:1052581/237‑1 (MQ=255)
                                                                                                                                                                atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAAAAAAATAAAAAAACTAGAGGTGTCATGATTAGGGAAAAGAATAAACAACAAGCAGTGAATAAGTTGACAGAGAATTTTGCTAACATTTCCGCCACGATTTCTTACCCAAATGCCTCTGACGGGGTGGGTAAAATAAAAAAACTTAAGGATGCCGCAAAGCCGGCGATggggga                                                           >  1:333808/1‑237 (MQ=255)
                                                                                                                                                                 ttaagggggaaaaaaaaTAAAAAACCAAATTTTTGGGGGAAGTTGGGGGGGGTGAATTTTTCCAGACAAATAAAAAAAGTGGAGTTGCCAGGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAAAGAGTTTGTCGCAAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGGCGATGGGGa                                                            <  1:325485/226‑1 (MQ=255)
                                                                                                                                                                 gtCACGGTGAGAAAAAATAAAAACACAATTGTTGTGTGGTGTTTGGTGGCGTAAAATTTTCCCAAACAAACACAAAATCTGGAGTTGCCATGGTAGGCAAAAGAAATAAGAAACCGGAAGTGAAAACGTGGACATGGATGGTCCCAATCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                            <  1:790916/236‑1 (MQ=255)
                                                                                                                                                                      ttggcaaaaaaaTAAAAACCCATATTTTATGTTGTAGTTGGGGGGCGTAATTTTTTAAACAAAAAAAAAAAATCTGGGGTTCCAATGTTGGGGGAAAGTAATAAGCAACAGGGTGTAAATAAGTAGACAGAGTTTGGCGCAAAGTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                     <  2:1077924/233‑1 (MQ=255)
                                                                                                                                                                       gTGGAAAAAAAGATAAACCATATTTTTAAGTGGAATTTTGTGGGGGTGAATTTTTCCAAACAAAAACAAAATCTGGGTTTGCCATGATGGGCAAAATAAAAAAGCAAAAGGCAGTAAATAGTTTGACATGGTTTGTCGCTACTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                     <  1:237497/238‑1 (MQ=255)
                                                                                                                                                                                           tcttttttAGGTGTAGTGTGTGGCGGTAATTTTTCCAAAAAAAATAAAAAAACGGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACGGGAAGTGAATAAGTTGAAAGAGATTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGacaa                                <  1:95589/236‑1 (MQ=255)
                                                                                                                                                                                           ctttttttAGTGGGTTTTTGGGGGGGGAATTTTTCACAGAAAAATAAAAAATGGGGATTCACCGTGATGGCAAAAAGAAATAAGAACACGGTAGTAAATAATTTGAGAGTGGTGGCCGCTAACTATACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGacaa                                <  2:1039425/236‑1 (MQ=255)
                                                                                                                                                                                           atttttttGGTGGTATTTTGTGGGGGAGAATTTTACCAAAAAAAAAAAAAAACTGGGTTTGCCATGAGGAGCAAAAAAAAAAAACAACGGGAAGTAAATAAGTGAACAGATATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGacaa                                <  2:333808/236‑1 (MQ=255)
                                                                                                                                                                                                                       gtataTTTTTCCAAAAAAATAAAAAAACTGGGTGTGCCTTGAGGAGTGAAGATAATAAGCAACTGGCAGGAGTATGTTGGAAGGAAGTTGCCCGAAACTTACCCTCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAATGATGCCGAAACGTCGGCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATACAAAAAGCGATTGACCTGAAt       <  2:486584/235‑1 (MQ=255)
                                                                                                                                                                                                                             tttttCCCAAAAAAAAAAAAAACTGGTGTTGCCATGATGAGCGAAAGAAAAAAACAACGGGCTGTGAATAGGTTGCAAGGTATGGCCGTTAACTTTACCGCCATGATTGCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcc  <  1:861262/239‑1 (MQ=255)
                                                                                                                                                                                                                                         cAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTa                                                  >  1:294178/1‑181 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                  
CCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCC  >  NC_000913/3206212‑3206666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: