Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,277,507	A→G	14.6%	E58G (GAG→GGG)	yhaV →	toxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,277,507	0	A	G	14.6%	214.6 / 3.7	97	E58G (GAG→GGG)	yhaV	toxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base A (32/49);  new base G (10/6);  total (42/55)
Fisher's exact test for biased strand distribution p-value = 1.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGT‑TGGGCGCTATATGCTCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAA  >  NC_000913/3277269‑3277740                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                acaaGGAAAAAAACTTGTCGCTGGCATGGACGTCAACATTGTTGATGGGTTTGGGGACGACGAATAAGGGATTTTCCACAAAGGT‑TTAATGGT‑TGGGCGCTATATGCTCATCCCTGTTTTC‑AGGAAACCTACGACGTTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAGTGATTgagga                                                                                                                                                                                                                                                 <  1:418140/239‑1 (MQ=255)  aagggAAAGAAACTTGGCGTTGGAATGGACGTCAACATGTATGATGATTTTGGCGACGACGAATAATGGATTTCCCACAAAGGC‑TTAATGGT‑TGGGCATTATATCCTAACCCCTTTTTCA‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGAACC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAGTGATTgaggag                                                                                                                                                                                                                                                <  1:955974/236‑1 (MQ=255)                 gTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATACTGGCTTTTCCACAAAGGG‑TTAATGGT‑TGGGCGCTATATGCACATCCCTGTTTTC‑AGGAAAACTACGACGCTTTAGTTGCCGAAGTCGACACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAACGCCGCCCCACAGTTATTGGCGGTAGAT‑ACA‑AAAGGGAGTGAGGAGcgagaaccggtaaa                                                                                                                                                                                                                                  >  2:812345/1‑225 (MQ=255)                       ggCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGT‑TGGGCGCTATATGCTCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAA‑T‑CCATCAAAGGATTTAGGAGGATAT‑ACCGGGCAATCcatca                                                                                                                                                                                                                          >  1:47776/1‑239 (MQ=255)                           tGGACGTCAACATTGATGATGAGATTTGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGT‑TGGGCGCTATATGCTCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCAtcatca                                                                                                                                                                                                                       <  1:804245/238‑1 (MQ=255)                                  cAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGT‑TGGGCGCTATATGCTCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCCCAAAGTTATTGGCGGTAG‑G‑CCACAAAATGATTGTGGGGCACAT‑CAAGGTccagtcactctcacccgact                                                                                                                                                                                                                >  1:592352/1‑219 (MQ=255)                                  aaagagacaggatgaGATTGGCGACGACGAATAAGGGATTTTCCAAAAGGGG‑TTAAGGGT‑TGGGCCCTAAAGGCTCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑Att                                                                                                                                                                                                              <  1:414169/229‑1 (MQ=255)                                       ttggtgagtgggtgggggacgacaaATAATGGTTTTTACAAAAAGGG‑TTAATGGT‑TGGGCGTTATATGCTCATCACTGTTGTC‑AGGAAACCGACGACGCTTTAGTGGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTc                                                                                                                                                                                                         <  2:432034/222‑1 (MQ=255)                                          atgatgaGATTGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGT‑TGGGCGCTATATGCTCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAA‑TACCACAAAG‑GATTGAGGAGGATAT‑TACGGGCAAATCATCATCAccagacatcccgcaag                                                                                                                                                                                                       >  1:386016/1‑225 (MQ=255)                                          atgatgaGATTGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGT‑TGGGCGCTATATGCTCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGGTG‑T‑CCACACAATGATTGAGGAGCGACA‑CACGGTCAAAccctacacacccaccatccctact                                                                                                                                                                                                        >  2:281795/1‑216 (MQ=255)                                                gggttGGGGAGGACGATTAAGTGATTTTCCACAAAGGT‑TTAATGGT‑TGGGCCCTATGTGCTCATCCCTTTTTTC‑AGGAAACCTACGACGTTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAAAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAg                                                                                                                                                                                                <  1:693653/235‑1 (MQ=255)                                                   tgggcagcaagaaattatGTATTTACCAAAAAGTG‑TTAATTGT‑GGGGCGTTTACAGCCAACCCATGTTTCA‑AGGAAACCGACGACGTTTTAGTTGCGGAAGTCGAAAAATTAAGGGAAAAAGATCC‑TGAAAATTATA‑AAAAAAAGGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGt                                                                                                                                                                                            <  2:47776/227‑1 (MQ=255)                                                    tGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGT‑TGGGCGCTATATGCTCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCCCAAAGTTATTGGCGGTAG‑T‑CCCTAAAAAGATTGAGGAGGCTAT‑CACGGGCAATCCACCATCACCGCC‑ATTCCGTC‑ATGGCAAGACGtt                                                                                                                                                                                           >  1:772907/1‑239 (MQ=255)                                                    tGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGT‑TGGGCGCTATATGCTCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAGGGATTGAGGAGCATAT‑CACGGTCAAACCATAATCACCCGC‑ATTCCGTC‑ATGGCCAGTCggt                                                                                                                                                                                           >  1:696416/1‑237 (MQ=255)                                                    tGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGT‑TGGGCGCTATATGCTCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑A‑CCATAAAAGGATTGAGGGGCATAT‑CACGGGCAATACCTCATCACCAGC‑ATTCCGGC‑CAGGCaaagactt                                                                                                                                                                                           >  1:51602/1‑233 (MQ=255)                                                       cgacgacgaATAATGGATTTTCCACAAAGGG‑TTAATGGT‑TGGGCGCTATATGCTCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAAAGATTTAGGGGCATAT‑CACGGGTAAACCCACATCACCCGCAATTCGGTC‑A‑GGCAAGtgagtag                                                                                                                                                                                         >  1:34176/1‑232 (MQ=255)                                                           gacgaATAATGGATTTTCCACAAAGGG‑TTAATGGT‑TGGGCGCTATATGCTCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCCTAAAGTGATTGAGGGGGCAAT‑CACGGCCAAACCCGCAACACCGGC‑ATTTCCCC‑ATGGCAAATGGTTAGGcctt                                                                                                                                                                                    >  1:817670/1‑236 (MQ=255)                                                           gacaaATAATGGATTTCCCACAAGGGG‑TTAATGGT‑TGGGGGTTATATGCTCACCCTTTTTTAC‑AGAAAACCTACGCGGTTTTAGTGGCCGAAGTCGGGACATTAAAGGAAAAAGATCC‑TGAAAATTATC‑AGAAAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGctct                                                                                                                                                                                    <  1:502701/239‑1 (MQ=255)                                                            acgaATAATGGATTTTCCACAAAGGG‑TTAATGGT‑TGGGCGCTATATGCTCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAGGGATTTAGGGGCAAAT‑ACCGGTCAATCCATCCTCAACGGC‑CTTCCGTC‑ATGGCAAGTCCTTTgggcctg                                                                                                                                                                                   >  1:262948/1‑234 (MQ=255)                                                               aataagGGATTTTCCAGAAAGGT‑TTAAGGGT‑GGGGCTGTATATGCGTATCACTGTTTGC‑AGGAACCCTCCGACACTTTAGTCGCCAAAGCCGAGACATTAAAGGGAAAGGACCG‑AAAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTAGTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGa                                                                                                                                                                                <  1:43421/239‑1 (MQ=255)                                                                attatgttttttCCA‑AAAAGG‑TTTATGGTGTGTGCGCTATGTGCCCATCCTTTTTTCC‑GGGAACCCTGCGTCGCTTTAGTGGCCGAAGTCTATACATTAAAGGGAAAAGATCC‑TGAAAATAAGC‑AGAAAAACGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGa                                                                                                                                                                                <  2:610958/230‑1 (MQ=255)                                                                ataatGGATTTTCCAAAAAGGG‑TTAATGGT‑TGGGCGCTATATGCCCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTGCCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAAAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGa                                                                                                                                                                                <  1:970801/238‑1 (MQ=255)                                                                aatatGGTTTTTCCAAAAAGGGTTTAAGGGT‑TGGGCGTTATGTGCTCATCCCTGTTTCC‑AGGAAACCCACGCCGTTTTAGTTGCCGAAGTGGAAAATATAAAGGGAAAAGACCC‑GAAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGa                                                                                                                                                                                <  2:614479/236‑1 (MQ=255)                                                                         tttCCACAAAGGG‑TTAATGGT‑TGGGCGCTATATGCTCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAATGATTTAGGAGCATAT‑CACGGGCAATCCCTCTTCCCCGGC‑CTTCCGTC‑ATGGCAAGTCGTTAGGTGCCGGGGaaaaa‑aaaaa                                                                                                                                                                     >  1:532005/1‑237 (MQ=255)                                                                         ttccaacaAGGGT‑TTAATGTT‑GGGGCTCTATATGCCCACCCTTTTTTAC‑AGGAACCCTCGCACGTTTTAGTTGCAAAAGCCGAGACATAAAAGGGAAAAGCTCC‑GAAAAATTAAA‑AGAAAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATGCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGa                                                                                                                                                                     <  2:34176/234‑1 (MQ=255)                                                                                 aaGGG‑TTAATGGT‑TGGGCGCTATATGCTCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCCTAAAGTGGTTTGGGGGGATAT‑CACGGGCAATCCCACATTACCCGC‑ATTCCGTC‑ATGGCAAGTGGTTAGGGCCTTGGGAAAAA‑AAAAACTGGTCAc                                                                                                                                                             >  1:498872/1‑239 (MQ=255)                                                                                             gggtGGGCGTTATAAGCTCATCCCTTTTTAC‑AGGAACCCTCCGCTCTTTTAGTTGCCGAAGTCAAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAATTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAAtt                                                                                                                                                  <  1:841365/236‑1 (MQ=255)                                                                                              gtgtgcccttttatCCTCAACCCGGTTTAC‑AGAAAACCTACGCAGCTTTAGTTGCCGAAGTCGAAACATTAAAGGGAAAAGATCC‑TGAAAATCATC‑AGAGAAAGTCCGCCCCAAAGTTAGTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAAtt                                                                                                                                                  <  1:157594/227‑1 (MQ=255)                                                                                                   gtgttattTGCTCA‑CCCTGTTTTCAAGGAACCTTCCGACGTTTTAGTTGCCGAATGCGAGAAATTAAAGGGAAAGTACTC‑GAAAATTTATA‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATTACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTg                                                                                                                                             <  1:513646/234‑1 (MQ=255)                                                                                                       tttttgcttctCCCTGTTTGT‑AGAAAACCTACGACGTTTGAGGTGCCAAAGTAGACACATTAAAGGGAAAATATCC‑TAAAAATTAGA‑AAAAAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑TCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGt                                                                                                                                        <  2:934850/229‑1 (MQ=255)                                                                                                       tataTGCTCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCCTAAAGAGATTTGGCGGCAGAT‑TACGGTCCAACCATCATCACCCGC‑ATCTCATCTATGGC‑AGACGTTGGGCCCTGGGGGAAAA‑AAAAACCCGTCCC‑GGGGaaaactagtggcatgc                                                                                                                                        >  2:671940/1‑227 (MQ=255)                                                                                                       tataTGCTCATCCCTGTTTGC‑AGGAATCCTACGACGCTTTAGTTGCCGAAGACGAGACATTAAGGGAAAAAGATCC‑TGAAAATTAGG‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGt                                                                                                                                        <  1:340337/239‑1 (MQ=255)                                                                                                         tatGCTCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCCTAAAAGGATTTAGGAGCATAT‑CCCGGTCAAACCATCATCAACCGC‑ATTCCCTC‑CTGGCCAATCGTTAGGGCCTGGGGAAAAT‑AAAAACTGGTCAC‑GGGGAAAATTTTGTGCTgtg                                                                                                                                        >  1:480707/1‑235 (MQ=255)                                                                                                                 tCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTTG‑G‑CCATAAAGTGATTGAAGAGCAAAT‑CACGGTCCATCCCTCATCACCCGC‑ATTACGTC‑AAGGCAAGACGTATGGACCTTGGGAAAAA‑AAAAAATGGTCACAGGGAAAAATATgggttggggggtatagg                                                                                                                                >  2:530481/1‑223 (MQ=255)                                                                                                                 tCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAATGATTTAGGAGCATAT‑CACGGGCCATCCATCATCACCCGC‑ATTCCCTC‑ATGGCCAGTCGTTAGGGTCTGGGGAAAAT‑AAAAACTGGTCCC‑GGGTAAAAATTGGTGCTGGT‑CGTTATCg                                                                                                                               >  1:756170/1‑237 (MQ=255)                                                                                                                 tCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAA‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGGTAGGCTCTGGGAAAAAT‑AAAGACTTGACAC‑GGGGAAAATTTGGGGATGGG‑CGGTATTGt                                                                                                                              >  1:854872/1‑238 (MQ=255)                                                                                                                 tCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGGAG‑A‑CCATAAAATGATTGCGGAGCATATACAC‑GTCAAACCATTATACCCGGC‑AATCCGAC‑ATCGCACGACGTTAGGCTCTGGGAAAAAA‑AAAAAACTGTCCC‑GGGGCAAAtttttgggggggagagatagt                                                                                                                               >  2:39937/1‑220 (MQ=255)                                                                                                                 tCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGAAG‑T‑CCCAAAAGTGATTTGGGAGACTAT‑CACAGGCAATCCCCCATCACCTGC‑CTTCCCGC‑ATGGCCAGGCGTTACGCCTTTGGAAAAAT‑AAAAACTGGTACA‑GGGGAAAATTTtgtgtaggcggtttttct                                                                                                                               >  1:518486/1‑224 (MQ=255)                                                                                                                  cccTGTTTTC‑AGGAAACCTACGGCCTTTTAGTTGCAGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAAAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCg                                                                                                                               <  1:851130/236‑1 (MQ=255)                                                                                                                             aGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑GCATAAAATGATTGAGGAGCACAT‑CAAGGTGCATCCCCCAGCCCCGGC‑ATTCCGTC‑TTGGCAAATGGTTAGGGTCTGGGCAAAAA‑AAAAAATCGGCCC‑GGGGAAAAATTGGTGGGGGT‑GGTTTTCGTCTCTTCttt                                                                                                                     >  2:851130/1‑236 (MQ=255)                                                                                                                             aGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAATGATTGAGGGGGATAT‑CACGGTCAATCCATCATCACCCGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGGAAAAT‑AAAAACTGGTCAC‑GGGGAAAAATTTGTGCTgtcggttttaggctctctttttgt                                                                                                                   >  1:562295/1‑216 (MQ=255)                                                                                                                                 aacctacttccttttttttGCCGAAGGCGAGAAAAAAAAGGTAACAGTTCC‑TAAAAATAACA‑AGAAAAAAGCCGCCACAAAGGTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTtata                                                                                                              <  2:368179/222‑1 (MQ=255)                                                                                                                                  aCCTACGTCGTTTTATTTGCCGATGTCGAGACATTAAGGTAAAAAGCTCA‑TGAAAATTCAA‑AAAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTAAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAg                                                                                                             <  2:265001/239‑1 (MQ=255)                                                                                                                                  aCCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAAAGATTGAGGGGCCTAT‑CACGGTCCAACCCTCATCACCGGC‑ATTCCGTC‑ATTGCACATGGTTTGGCTCTGGGGAAAAT‑AAAAACTGGTCCC‑GGGGAAAATTTTGTGCTGGT‑CGTTATCGTCTCTTTTTTCTTtttat                                                                                                             >  1:595697/1‑238 (MQ=255)                                                                                                                                     tACGAGGCTTAGGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑GGAAAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCGATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGa                                                                                                          <  1:340188/239‑1 (MQ=255)                                                                                                                                           gCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCa                                                                                                                                                                                                  <  1:483727/148‑1 (MQ=255)                                                                                                                                              ttATTTGCCGAAAGCGAACAATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAgaga                                                                                                  <  1:386419/238‑1 (MQ=255)                                                                                                                                                  ttGCCAAAGTAGAGACATTAAAGGGAAAAGATCA‑GGAAAATTATA‑AGAAAAATGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGt                                                                                              <  1:318995/238‑1 (MQ=255)                                                                                                                                                   tGCCGAAGTCGAGACATTAAAGGGAAAAGCTCC‑GAAAATTTATA‑AGAAAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGtca                                                                                            <  1:39937/239‑1 (MQ=255)                                                                                                                                                    tgcgaaggggggaaaatAAAGGGAAAAAATCCATAAAAATTACG‑AGAAAAAGGCCGCCACAAAGTTAATGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATGCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGtca                                                                                            <  2:480707/223‑1 (MQ=255)                                                                                                                                                    gcaaaaGTCGACACTATAAGGGAAAAAGATCC‑TGAAAATTAAC‑AAAAAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGtcat                                                                                           <  2:928017/235‑1 (MQ=255)                                                                                                                                                    gCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCCTAAAATGATTGGGGAGCAAAT‑CCCGGTCAATTCATACTCACCCGC‑ATTCCGTC‑ATGGCACATCGTTAAGCGCTGGGAAAAAT‑AAAGACTGGACCC‑GGGTCAAATTTGGTGATGGT‑CGTTATACTCTCTCTTTTCGTTTTAGTGACAAAGAGAAAGtcat                                                                                           >  1:455312/1‑239 (MQ=255)                                                                                                                                                     ccGAAGGCGAGACATTAAAGGAAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTta                                                                                                                <  1:875335/217‑1 (MQ=255)                                                                                                                                                        atgtgggaaCATAAAAGGGAAAAATTTC‑TGAAATTTAAC‑AGAAAAAGGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAtt                                                                                       <  2:498872/231‑1 (MQ=255)                                                                                                                                                               gaCATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCCTAAAGGGATTGAGGAGCATAT‑CACGGTCAATTCATCATTACCGGC‑CTTTCGTC‑ACGGCCAATCGTTAGGGCCTGGGAAAAAA‑AAAAACCGGGCCC‑GGGGTAAATTTTTTG‑TGGTGCGTTTTAGTCTCTTCTTTCTTTTTATGGaaaaaaacgtgcctttttaacaattcc                                                                                >  2:875335/1‑217 (MQ=255)                                                                                                                                                                  aTTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑G‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCCCTGGGAAAAAA‑AAAGACTGGTCCC‑GGGGAAAATTTGGTGCTGGT‑CGTTATTGGCTCTTCTTTCGTTTTTGGGAAAAAGAGAAAGGCATCATTCTtggatgga                                                                             >  1:141665/1‑239 (MQ=255)                                                                                                                                                                     aaaGGGAAAAGCTCC‑TGAAAATTATC‑AGAAAAAAGCCGCCACAAAGGTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCAGAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGa                                                                          <  1:121005/239‑1 (MQ=255)                                                                                                                                                                     aaaGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAAAGATTGAGGAGCATAT‑CACGGTCAAAACATCATCACCGGC‑ATTCCGTC‑ATGGCAAGACGTTAGGCTTTGGGAAAAAT‑AAAAACAGGTGAC‑GGGGCAAAATTTGTGCTGGT‑CGTTATTGTCTCTTTTTTCGTTTTAGGGAAAAAAAGAAAGGCATAATTTTTGGGTGGGTGa                                                                          >  1:982528/1‑239 (MQ=255)                                                                                                                                                                     aaaGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CAATTAAAAGAATGAGGGGCCAAT‑ACCGGTCAATCCATCATCAACCGC‑ATTTCGTC‑ATGGGGAGACGTATGGGCCTCGGGACAAT‑AAAAACAGGTCAC‑CGGTACACATTGTGACTGGT‑AGTTTACGTCACCTTCTTCTTTTTATGGAAAAAAAGAAAGCGATCATTCTAggagggtgag                                                                          >  2:10785/1‑235 (MQ=255)                                                                                                                                                                          aaaaaTATCC‑GTAAAATTATA‑AGAAAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGAATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGGTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCCCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGa                                                                      <  2:854872/237‑1 (MQ=255)                                                                                                                                                                           aaaaaaGCC‑TGAAAAATTTAAAGAAAAAACCCCCCACAAAGGTTTTGGCGGTTG‑T‑CCCTAAAGTGATTGAGGGGCATAT‑CACGGTCAATCCAACATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGa                                                                      <  2:528439/238‑1 (MQ=255)                                                                                                                                                                           aaaaGATCC‑TGAAAATTATA‑AGAAAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGa                                                                      <  1:905062/237‑1 (MQ=255)                                                                                                                                                                            aaaGATCC‑TGAGAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAGCGATTGAGGAGGATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTGGTTAGGCGCTGGGGAAAAT‑AAAGAACGGGCCC‑GGGGAAAAATTTGGGACGGG‑CGGTATCGCATCTTTTTTTTGTATAGGGAAAAAAAAAAAAAGAATATTCTGggaggggagagaagaga                                                                   >  2:575203/1‑228 (MQ=255)                                                                                                                                                                            aaaGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGGAG‑T‑CCATAAAATGATTGAGGAGCAAAT‑CAACGGCAATACCTCATCACCGGC‑CTTACGAG‑ATGGCAAGGCGCTAAGGCCTGGGAAAAATAAAAGAAGGGGCAG‑GGGAACAATTTGG‑GCCGGT‑CGTTATTGCTTCTTCTTTCGTTTTAGTGAACAAAAAAAAAAAATCATTCTGggttgtgttaaaggaaa                                                                   >  2:860739/1‑227 (MQ=255)                                                                                                                                                                                 tCC‑TGAAAATTACA‑AGAGAAAGGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCAGCAGCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAaca                                                              <  1:860739/239‑1 (MQ=255)                                                                                                                                                                                    ctaaaaaTTATC‑AGAGAAAGGCCGCCACAAAGTTAGTGGCGGTAG‑G‑CCATAAAGTGATTGAGGAGAATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACAct                                                            <  1:1024166/236‑1 (MQ=255)                                                                                                                                                                                     tGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGCAG‑G‑CCCTAAAGAGAGTGAGGAGCAAAA‑CACGGGCCAACCCTCATCACCGGC‑ATTCCCTC‑CAGGCCAACCGTTAGGCTCTGGGGAAAAA‑AAAAACAGGGTCC‑GGGTAAAAATTGTTGTTGGT‑CGTTTTAGTCTCTTTTTTCTCTATATTGGAAAAGAGAAAAGCATAATTCTGgggtgggtaacaaatgaaaaaacac                                                           >  2:722008/1‑220 (MQ=255)                                                                                                                                                                                      gAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCcatcat                                                                                                                                                                                                                         <  2:929107/85‑1 (MQ=255)                                                                                                                                                                                      gAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAATGATTGAGGAGCATAT‑CACGGTCAATCcatcat                                                                                                                                                                                                                         >  1:929107/1‑85 (MQ=255)                                                                                                                                                                                                 agaaAAAAGCCGCCCAAAAGTTATTGGCGGTAG‑T‑CCATAAAGTGATGGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTAc                                                <  2:756170/239‑1 (MQ=255)                                                                                                                                                                                                   agaAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCCTAAAATGATTGAGGAGCATAT‑CACGGGCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGAATGGTCAC‑GGGGAAAAATTTGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTTGGGTGGATGAACGATGAAAAAACACCTCGAACCCACgg                                              >  1:371216/1‑239 (MQ=255)                                                                                                                                                                                                    gaAAAGCCGCCACAAAGTTATTGGCGGTAG‑A‑CCATAAAGTGATTGAGGAGCATAT‑CACGGGCCATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAATCGTTAGGCTCTGGGGAAAAT‑AAAAAATGGTCAC‑GGGTAAAATTTTGTGCTGGT‑CGTTATCGTCTCTTTTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGAGTAAAAAActcccgggccctacggc                                             >  1:685792/1‑225 (MQ=255)                                                                                                                                                                                                             cccaaaaGTTTTTTGGGGTAT‑T‑CCATAAATTGATTGATGGGTATTT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACa                                    <  2:51602/235‑1 (MQ=255)                                                                                                                                                                                                             cccaaaaGTTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACa                                    <  2:262948/235‑1 (MQ=255)                                                                                                                                                                                                             ccACAAAGTTATTGGCGTGTG‑T‑CCATAAAGTGAGTGAGGGGCCTAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGGCTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACa                                    <  2:597068/239‑1 (MQ=255)                                                                                                                                                                                                              cacaAAGTTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGGCAATCCAGCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACGGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACa                                    <  1:1075032/238‑1 (MQ=255)                                                                                                                                                                                                               caaaaGTTTTTGGGGGTAG‑T‑CCATAAAGTGTTTGAGGGGCATAT‑CCCGGGTAAACCAACATCACCGGC‑ATGCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGa                                  <  2:595697/237‑1 (MQ=255)                                                                                                                                                                                                                  aaGTTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGc                               <  1:474564/239‑1 (MQ=255)                                                                                                                                                                                                                   aGTTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGtca                                                                                            <  1:173375/177‑1 (MQ=255)                                                                                                                                                                                                                     ttATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTg                                                                                                                                             <  1:478991/127‑1 (MQ=255)                                                                                                                                                                                                                     ttAATGGCGGTAT‑T‑CCATAAAGTGAGTGAGGAGCATAT‑CACGGTCAATCCAGCATCACCGGC‑AGTCCGTC‑ATGGCAAGTCCTTAGGCTCAGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTGGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTAc                                                <  1:205988/219‑1 (MQ=255)                                                                                                                                                                                                                      tATTGGCCGTAG‑T‑CCATGAAGTGATTGATGAACATAT‑CACGGTCAATCCCACATCACCTGC‑ATTCAGTC‑ATGGCAGGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTGGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAATCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCt                             <  1:983536/237‑1 (MQ=255)                                                                                                                                                                                                                       aTTGGAGGTAG‑G‑CCAAAAAGTGATTGAGGAGCATAT‑CCCGGTCAATCCATCATCAACGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCtata                          <  2:772907/239‑1 (MQ=255)                                                                                                                                                                                                                             ggTAG‑G‑CCATAAAGTGATTGAGGAGCATAT‑CCCGGTCAATCCATCATCCCCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTa                     <  1:690838/238‑1 (MQ=255)                                                                                                                                                                                                                               tAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTAtt                   <  1:381680/238‑1 (MQ=255)                                                                                                                                                                                                                                   t‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATAACGGATTCAGc               >  1:869973/1‑239 (MQ=255)                                                                                                                                                                                                                                     ccATAAAGTGTTTGAGGGGCATAT‑CCGGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCa              <  2:869973/239‑1 (MQ=255)                                                                                                                                                                                                                                      cATAAAGTGATTGAGGGGCATAT‑GCCGGTCAATCCATCATCCCCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCa              <  1:779348/238‑1 (MQ=255)                                                                                                                                                                                                                                             tGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACa                                    <  1:808298/209‑1 (MQ=255)                                                                                                                                                                                                                                             tGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTa      >  1:127306/1‑239 (MQ=255)                                                                                                                                                                                                                                               aTTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAACAACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGAATTCAGCAAAATGTTaaa    >  1:288665/1‑239 (MQ=255)                                                                                                                                                                                                                                               aTTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTTTGCGCACCTACGGTAAAAAAACa                                    >  2:1027025/1‑207 (MQ=255)                                                                                                                                                                                                                                               aTTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACCAGATGCCTATACCGCATTCAGCAAAATGTTaaa    >  1:963354/1‑239 (MQ=255)                                                                                                                                                                                                                                               aTTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGCATTCAGCAAAATGTTaaa    >  1:823326/1‑239 (MQ=255)                                                                                                                                                                                                                                               aTTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGAC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACa                                    <  1:1027025/207‑1 (MQ=255)                                                                                                                                                                                                                                                ttGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCtcttct                                                                                                                       >  2:70902/1‑123 (MQ=255)                                                                                                                                                                                                                                                ttGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCtcttct                                                                                                                       <  1:70902/123‑1 (MQ=255)                                                                                                                                                                                                                                                ttGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTaaaa   >  1:197757/1‑239 (MQ=255)                                                                                                                                                                                                                                                 tGAGGAGCATAT‑CACGGTCAAACCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTaaaaa  <  1:134698/239‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                ACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGT‑TGGGCGCTATATGCTCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAG‑T‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAA  >  NC_000913/3277269‑3277740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑