Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,277,523 T→A 11.4% N63K (AAT→AAA)  yhaV → toxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,277,5230TA11.4% 270.5 / 3.3 101N63K (AAT→AAA) yhaVtoxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base T (35/53);  new base A (11/2);  total (46/55)
Fisher's exact test for biased strand distribution p-value = 2.88e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

GGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGT‑TGGGCGCTATATGCTCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCG  >  NC_000913/3277291‑3277758
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                
ggCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGT‑TGGGCGCTATATGCTCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAAT‑CCATCAAAGGATTTAGGAGGATAT‑ACCGGGCAATCcatca                                                                                                                                                                                                                                            >  1:47776/1‑239 (MQ=255)
    tGGACGTCAACATTGATGATGAGATTTGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGT‑TGGGCGCTATATGCTCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCAtcatca                                                                                                                                                                                                                                         <  1:804245/238‑1 (MQ=255)
           aaagagacaggatgaGATTGGCGACGACGAATAAGGGATTTTCCAAAAGGGG‑TTAAGGGT‑TGGGCCCTAAAGGCTCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑Att                                                                                                                                                                                                                                <  1:414169/229‑1 (MQ=255)
                ttggtgagtgggtgggggacgacaaATAATGGTTTTTACAAAAAGGG‑TTAATGGT‑TGGGCGTTATATGCTCATCACTGTTGTC‑AGGAAACCGACGACGCTTTAGTGGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTc                                                                                                                                                                                                                           <  2:432034/222‑1 (MQ=255)
                   atgatgaGATTGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGT‑TGGGCGCTATATGCTCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAATACCACAAAG‑GATTGAGGAGGATAT‑TACGGGCAAATCATCATCAccagacatcccgcaag                                                                                                                                                                                                                         >  1:386016/1‑225 (MQ=255)
                   atgatgaGATTGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGT‑TGGGCGCTATATGCTCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGGTGT‑CCACACAATGATTGAGGAGCGACA‑CACGGTCAAAccctacacacccaccatccctact                                                                                                                                                                                                                          >  2:281795/1‑216 (MQ=255)
                         gggttGGGGAGGACGATTAAGTGATTTTCCACAAAGGT‑TTAATGGT‑TGGGCCCTATGTGCTCATCCCTTTTTTC‑AGGAAACCTACGACGTTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAAAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAg                                                                                                                                                                                                                  <  1:693653/235‑1 (MQ=255)
                            tgggcagcaagaaattatGTATTTACCAAAAAGTG‑TTAATTGT‑GGGGCGTTTACAGCCAACCCATGTTTCA‑AGGAAACCGACGACGTTTTAGTTGCGGAAGTCGAAAAATTAAGGGAAAAAGATCC‑TGAAAATTATA‑AAAAAAAGGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGt                                                                                                                                                                                                              <  2:47776/227‑1 (MQ=255)
                             tGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGT‑TGGGCGCTATATGCTCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCCCAAAGTTATTGGCGGTAGT‑CCCTAAAAAGATTGAGGAGGCTAT‑CACGGGCAATCCACCATCACCGCC‑ATTCCGTC‑ATGGCAAGACGtt                                                                                                                                                                                                             >  1:772907/1‑239 (MQ=255)
                             tGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGT‑TGGGCGCTATATGCTCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAGGGATTGAGGAGCATAT‑CACGGTCAAACCATAATCACCCGC‑ATTCCGTC‑ATGGCCAGTCggt                                                                                                                                                                                                             >  1:696416/1‑237 (MQ=255)
                             tGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGT‑TGGGCGCTATATGCTCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGA‑CCATAAAAGGATTGAGGGGCATAT‑CACGGGCAATACCTCATCACCAGC‑ATTCCGGC‑CAGGCaaagactt                                                                                                                                                                                                             >  1:51602/1‑233 (MQ=255)
                                cgacgacgaATAATGGATTTTCCACAAAGGG‑TTAATGGT‑TGGGCGCTATATGCTCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAAAGATTTAGGGGCATAT‑CACGGGTAAACCCACATCACCCGCAATTCGGTC‑A‑GGCAAGtgagtag                                                                                                                                                                                                           >  1:34176/1‑232 (MQ=255)
                                    gacaaATAATGGATTTCCCACAAGGGG‑TTAATGGT‑TGGGGGTTATATGCTCACCCTTTTTTAC‑AGAAAACCTACGCGGTTTTAGTGGCCGAAGTCGGGACATTAAAGGAAAAAGATCC‑TGAAAATTATC‑AGAAAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGctct                                                                                                                                                                                                      <  1:502701/239‑1 (MQ=255)
                                     acgaATAATGGATTTTCCACAAAGGG‑TTAATGGT‑TGGGCGCTATATGCTCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAGGGATTTAGGGGCAAAT‑ACCGGTCAATCCATCCTCAACGGC‑CTTCCGTC‑ATGGCAAGTCCTTTgggcctg                                                                                                                                                                                                     >  1:262948/1‑234 (MQ=255)
                                        aataagGGATTTTCCAGAAAGGT‑TTAAGGGT‑GGGGCTGTATATGCGTATCACTGTTTGC‑AGGAACCCTCCGACACTTTAGTCGCCAAAGCCGAGACATTAAAGGGAAAGGACCG‑AAAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTAGTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGa                                                                                                                                                                                                  <  1:43421/239‑1 (MQ=255)
                                         attatgttttttCCA‑AAAAGG‑TTTATGGTGTGTGCGCTATGTGCCCATCCTTTTTTCC‑GGGAACCCTGCGTCGCTTTAGTGGCCGAAGTCTATACATTAAAGGGAAAAGATCC‑TGAAAATAAGC‑AGAAAAACGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGa                                                                                                                                                                                                  <  2:610958/230‑1 (MQ=255)
                                         ataatGGATTTTCCAAAAAGGG‑TTAATGGT‑TGGGCGCTATATGCCCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTGCCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAAAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGa                                                                                                                                                                                                  <  1:970801/238‑1 (MQ=255)
                                         aatatGGTTTTTCCAAAAAGGGTTTAAGGGT‑TGGGCGTTATGTGCTCATCCCTGTTTCC‑AGGAAACCCACGCCGTTTTAGTTGCCGAAGTGGAAAATATAAAGGGAAAAGACCC‑GAAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGa                                                                                                                                                                                                  <  2:614479/236‑1 (MQ=255)
                                                  tttCCACAAAGGG‑TTAATGGT‑TGGGCGCTATATGCTCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAATGATTTAGGAGCATAT‑CACGGGCAATCCCTCTTCCCCGGC‑CTTCCGTC‑ATGGCAAGTCGTTAGGTGCCGGGGaaaaa‑aaaaa                                                                                                                                                                                       >  1:532005/1‑237 (MQ=255)
                                                  ttccaacaAGGGT‑TTAATGTT‑GGGGCTCTATATGCCCACCCTTTTTTAC‑AGGAACCCTCGCACGTTTTAGTTGCAAAAGCCGAGACATAAAAGGGAAAAGCTCC‑GAAAAATTAAA‑AGAAAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATGCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGa                                                                                                                                                                                       <  2:34176/234‑1 (MQ=255)
                                                          aaGGG‑TTAATGGT‑TGGGCGCTATATGCTCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCCTAAAGTGGTTTGGGGGGATAT‑CACGGGCAATCCCACATTACCCGC‑ATTCCGTC‑ATGGCAAGTGGTTAGGGCCTTGGGAAAAA‑AAAAACTGGTCAc                                                                                                                                                                               >  1:498872/1‑239 (MQ=255)
                                                                      gggtGGGCGTTATAAGCTCATCCCTTTTTAC‑AGGAACCCTCCGCTCTTTTAGTTGCCGAAGTCAAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAATTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAAtt                                                                                                                                                                    <  1:841365/236‑1 (MQ=255)
                                                                       gtgtgcccttttatCCTCAACCCGGTTTAC‑AGAAAACCTACGCAGCTTTAGTTGCCGAAGTCGAAACATTAAAGGGAAAAGATCC‑TGAAAATCATC‑AGAGAAAGTCCGCCCCAAAGTTAGTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAAtt                                                                                                                                                                    <  1:157594/227‑1 (MQ=255)
                                                                            gtgttattTGCTCA‑CCCTGTTTTCAAGGAACCTTCCGACGTTTTAGTTGCCGAATGCGAGAAATTAAAGGGAAAGTACTC‑GAAAATTTATA‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATTACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTg                                                                                                                                                               <  1:513646/234‑1 (MQ=255)
                                                                                tataTGCTCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCCTAAAGAGATTTGGCGGCAGAT‑TACGGTCCAACCATCATCACCCGC‑ATCTCATCTATGGC‑AGACGTTGGGCCCTGGGGGAAAA‑AAAAACCCGTCCC‑GGGGaaaactagtggcatgc                                                                                                                                                          >  2:671940/1‑227 (MQ=255)
                                                                                tataTGCTCATCCCTGTTTGC‑AGGAATCCTACGACGCTTTAGTTGCCGAAGACGAGACATTAAGGGAAAAAGATCC‑TGAAAATTAGG‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGt                                                                                                                                                          <  1:340337/239‑1 (MQ=255)
                                                                                  tatGCTCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCCTAAAAGGATTTAGGAGCATAT‑CCCGGTCAAACCATCATCAACCGC‑ATTCCCTC‑CTGGCCAATCGTTAGGGCCTGGGGAAAAT‑AAAAACTGGTCAC‑GGGGAAAATTTTGTGCTgtg                                                                                                                                                          >  1:480707/1‑235 (MQ=255)
                                                                                          tCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTTGG‑CCATAAAGTGATTGAAGAGCAAAT‑CACGGTCCATCCCTCATCACCCGC‑ATTACGTC‑AAGGCAAGACGTATGGACCTTGGGAAAAA‑AAAAAATGGTCACAGGGAAAAATATgggttggggggtatagg                                                                                                                                                  >  2:530481/1‑223 (MQ=255)
                                                                                          tCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAATGATTTAGGAGCATAT‑CACGGGCCATCCATCATCACCCGC‑ATTCCCTC‑ATGGCCAGTCGTTAGGGTCTGGGGAAAAT‑AAAAACTGGTCCC‑GGGTAAAAATTGGTGCTGGT‑CGTTATCg                                                                                                                                                 >  1:756170/1‑237 (MQ=255)
                                                                                          tCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAAT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGGTAGGCTCTGGGAAAAAT‑AAAGACTTGACAC‑GGGGAAAATTTGGGGATGGG‑CGGTATTGt                                                                                                                                                >  1:854872/1‑238 (MQ=255)
                                                                                          tCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGGAGA‑CCATAAAATGATTGCGGAGCATATACAC‑GTCAAACCATTATACCCGGC‑AATCCGAC‑ATCGCACGACGTTAGGCTCTGGGAAAAAA‑AAAAAACTGTCCC‑GGGGCAAAtttttgggggggagagatagt                                                                                                                                                 >  2:39937/1‑220 (MQ=255)
                                                                                          tCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGAAGT‑CCCAAAAGTGATTTGGGAGACTAT‑CACAGGCAATCCCCCATCACCTGC‑CTTCCCGC‑ATGGCCAGGCGTTACGCCTTTGGAAAAAT‑AAAAACTGGTACA‑GGGGAAAATTTtgtgtaggcggtttttct                                                                                                                                                 >  1:518486/1‑224 (MQ=255)
                                                                                           cccTGTTTTC‑AGGAAACCTACGGCCTTTTAGTTGCAGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAAAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCg                                                                                                                                                 <  1:851130/236‑1 (MQ=255)
                                                                                                      aGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑GCATAAAATGATTGAGGAGCACAT‑CAAGGTGCATCCCCCAGCCCCGGC‑ATTCCGTC‑TTGGCAAATGGTTAGGGTCTGGGCAAAAA‑AAAAAATCGGCCC‑GGGGAAAAATTGGTGGGGGT‑GGTTTTCGTCTCTTCttt                                                                                                                                       >  2:851130/1‑236 (MQ=255)
                                                                                                      aGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAATGATTGAGGGGGATAT‑CACGGTCAATCCATCATCACCCGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGGAAAAT‑AAAAACTGGTCAC‑GGGGAAAAATTTGTGCTgtcggttttaggctctctttttgt                                                                                                                                     >  1:562295/1‑216 (MQ=255)
                                                                                                          aacctacttccttttttttGCCGAAGGCGAGAAAAAAAAGGTAACAGTTCC‑TAAAAATAACA‑AGAAAAAAGCCGCCACAAAGGTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTtata                                                                                                                                <  2:368179/222‑1 (MQ=255)
                                                                                                           aCCTACGTCGTTTTATTTGCCGATGTCGAGACATTAAGGTAAAAAGCTCA‑TGAAAATTCAA‑AAAGAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTAAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAg                                                                                                                               <  2:265001/239‑1 (MQ=255)
                                                                                                           aCCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAAAGATTGAGGGGCCTAT‑CACGGTCCAACCCTCATCACCGGC‑ATTCCGTC‑ATTGCACATGGTTTGGCTCTGGGGAAAAT‑AAAAACTGGTCCC‑GGGGAAAATTTTGTGCTGGT‑CGTTATCGTCTCTTTTTTCTTtttat                                                                                                                               >  1:595697/1‑238 (MQ=255)
                                                                                                              tACGAGGCTTAGGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑GGAAAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCGATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGa                                                                                                                            <  1:340188/239‑1 (MQ=255)
                                                                                                                    gCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCa                                                                                                                                                                                                                    <  1:483727/148‑1 (MQ=255)
                                                                                                                       ttATTTGCCGAAAGCGAACAATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAgaga                                                                                                                    <  1:386419/238‑1 (MQ=255)
                                                                                                                           ttGCCAAAGTAGAGACATTAAAGGGAAAAGATCA‑GGAAAATTATA‑AGAAAAATGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGt                                                                                                                <  1:318995/238‑1 (MQ=255)
                                                                                                                            tGCCGAAGTCGAGACATTAAAGGGAAAAGCTCC‑GAAAATTTATA‑AGAAAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGtca                                                                                                              <  1:39937/239‑1 (MQ=255)
                                                                                                                             tgcgaaggggggaaaatAAAGGGAAAAAATCCATAAAAATTACG‑AGAAAAAGGCCGCCACAAAGTTAATGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATGCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGtca                                                                                                              <  2:480707/223‑1 (MQ=255)
                                                                                                                             gcaaaaGTCGACACTATAAGGGAAAAAGATCC‑TGAAAATTAAC‑AAAAAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGtcat                                                                                                             <  2:928017/235‑1 (MQ=255)
                                                                                                                             gCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCCTAAAATGATTGGGGAGCAAAT‑CCCGGTCAATTCATACTCACCCGC‑ATTCCGTC‑ATGGCACATCGTTAAGCGCTGGGAAAAAT‑AAAGACTGGACCC‑GGGTCAAATTTGGTGATGGT‑CGTTATACTCTCTCTTTTCGTTTTAGTGACAAAGAGAAAGtcat                                                                                                             >  1:455312/1‑239 (MQ=255)
                                                                                                                              ccGAAGGCGAGACATTAAAGGAAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTta                                                                                                                                  <  1:875335/217‑1 (MQ=255)
                                                                                                                                 atgtgggaaCATAAAAGGGAAAAATTTC‑TGAAATTTAAC‑AGAAAAAGGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAtt                                                                                                         <  2:498872/231‑1 (MQ=255)
                                                                                                                                        gaCATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCCTAAAGGGATTGAGGAGCATAT‑CACGGTCAATTCATCATTACCGGC‑CTTTCGTC‑ACGGCCAATCGTTAGGGCCTGGGAAAAAA‑AAAAACCGGGCCC‑GGGGTAAATTTTTTG‑TGGTGCGTTTTAGTCTCTTCTTTCTTTTTATGGaaaaaaacgtgcctttttaacaattcc                                                                                                  >  2:875335/1‑217 (MQ=255)
                                                                                                                                           aTTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGG‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCCCTGGGAAAAAA‑AAAGACTGGTCCC‑GGGGAAAATTTGGTGCTGGT‑CGTTATTGGCTCTTCTTTCGTTTTTGGGAAAAAGAGAAAGGCATCATTCTtggatgga                                                                                               >  1:141665/1‑239 (MQ=255)
                                                                                                                                              aaaGGGAAAAGCTCC‑TGAAAATTATC‑AGAAAAAAGCCGCCACAAAGGTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCAGAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGa                                                                                            <  1:121005/239‑1 (MQ=255)
                                                                                                                                              aaaGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAAAGATTGAGGAGCATAT‑CACGGTCAAAACATCATCACCGGC‑ATTCCGTC‑ATGGCAAGACGTTAGGCTTTGGGAAAAAT‑AAAAACAGGTGAC‑GGGGCAAAATTTGTGCTGGT‑CGTTATTGTCTCTTTTTTCGTTTTAGGGAAAAAAAGAAAGGCATAATTTTTGGGTGGGTGa                                                                                            >  1:982528/1‑239 (MQ=255)
                                                                                                                                              aaaGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CAATTAAAAGAATGAGGGGCCAAT‑ACCGGTCAATCCATCATCAACCGC‑ATTTCGTC‑ATGGGGAGACGTATGGGCCTCGGGACAAT‑AAAAACAGGTCAC‑CGGTACACATTGTGACTGGT‑AGTTTACGTCACCTTCTTCTTTTTATGGAAAAAAAGAAAGCGATCATTCTAggagggtgag                                                                                            >  2:10785/1‑235 (MQ=255)
                                                                                                                                                   aaaaaTATCC‑GTAAAATTATA‑AGAAAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGAATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGGTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCCCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGa                                                                                        <  2:854872/237‑1 (MQ=255)
                                                                                                                                                    aaaaaaGCC‑TGAAAAATTTAAAGAAAAAACCCCCCACAAAGGTTTTGGCGGTTGT‑CCCTAAAGTGATTGAGGGGCATAT‑CACGGTCAATCCAACATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGa                                                                                        <  2:528439/238‑1 (MQ=255)
                                                                                                                                                    aaaaGATCC‑TGAAAATTATA‑AGAAAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGa                                                                                        <  1:905062/237‑1 (MQ=255)
                                                                                                                                                     aaaGATCC‑TGAGAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAGCGATTGAGGAGGATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTGGTTAGGCGCTGGGGAAAAT‑AAAGAACGGGCCC‑GGGGAAAAATTTGGGACGGG‑CGGTATCGCATCTTTTTTTTGTATAGGGAAAAAAAAAAAAAGAATATTCTGggaggggagagaagaga                                                                                     >  2:575203/1‑228 (MQ=255)
                                                                                                                                                     aaaGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGGAGT‑CCATAAAATGATTGAGGAGCAAAT‑CAACGGCAATACCTCATCACCGGC‑CTTACGAG‑ATGGCAAGGCGCTAAGGCCTGGGAAAAATAAAAGAAGGGGCAG‑GGGAACAATTTGG‑GCCGGT‑CGTTATTGCTTCTTCTTTCGTTTTAGTGAACAAAAAAAAAAAATCATTCTGggttgtgttaaaggaaa                                                                                     >  2:860739/1‑227 (MQ=255)
                                                                                                                                                          tCC‑TGAAAATTACA‑AGAGAAAGGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCAGCAGCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAaca                                                                                <  1:860739/239‑1 (MQ=255)
                                                                                                                                                             ctaaaaaTTATC‑AGAGAAAGGCCGCCACAAAGTTAGTGGCGGTAGG‑CCATAAAGTGATTGAGGAGAATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACAct                                                                              <  1:1024166/236‑1 (MQ=255)
                                                                                                                                                              tGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGCAGG‑CCCTAAAGAGAGTGAGGAGCAAAA‑CACGGGCCAACCCTCATCACCGGC‑ATTCCCTC‑CAGGCCAACCGTTAGGCTCTGGGGAAAAA‑AAAAACAGGGTCC‑GGGTAAAAATTGTTGTTGGT‑CGTTTTAGTCTCTTTTTTCTCTATATTGGAAAAGAGAAAAGCATAATTCTGgggtgggtaacaaatgaaaaaacac                                                                             >  2:722008/1‑220 (MQ=255)
                                                                                                                                                               gAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCcatcat                                                                                                                                                                                                                                           <  2:929107/85‑1 (MQ=255)
                                                                                                                                                               gAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAATGATTGAGGAGCATAT‑CACGGTCAATCcatcat                                                                                                                                                                                                                                           >  1:929107/1‑85 (MQ=255)
                                                                                                                                                                          agaaAAAAGCCGCCCAAAAGTTATTGGCGGTAGT‑CCATAAAGTGATGGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTAc                                                                  <  2:756170/239‑1 (MQ=255)
                                                                                                                                                                            agaAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCCTAAAATGATTGAGGAGCATAT‑CACGGGCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGAATGGTCAC‑GGGGAAAAATTTGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTTGGGTGGATGAACGATGAAAAAACACCTCGAACCCACgg                                                                >  1:371216/1‑239 (MQ=255)
                                                                                                                                                                             gaAAAGCCGCCACAAAGTTATTGGCGGTAGA‑CCATAAAGTGATTGAGGAGCATAT‑CACGGGCCATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAATCGTTAGGCTCTGGGGAAAAT‑AAAAAATGGTCAC‑GGGTAAAATTTTGTGCTGGT‑CGTTATCGTCTCTTTTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGAGTAAAAAActcccgggccctacggc                                                               >  1:685792/1‑225 (MQ=255)
                                                                                                                                                                                      cccaaaaGTTTTTTGGGGTATT‑CCATAAATTGATTGATGGGTATTT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACa                                                      <  2:51602/235‑1 (MQ=255)
                                                                                                                                                                                      cccaaaaGTTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACa                                                      <  2:262948/235‑1 (MQ=255)
                                                                                                                                                                                      ccACAAAGTTATTGGCGTGTGT‑CCATAAAGTGAGTGAGGGGCCTAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGGCTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACa                                                      <  2:597068/239‑1 (MQ=255)
                                                                                                                                                                                       cacaAAGTTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGGCAATCCAGCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACGGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACa                                                      <  1:1075032/238‑1 (MQ=255)
                                                                                                                                                                                        caaaaGTTTTTGGGGGTAGT‑CCATAAAGTGTTTGAGGGGCATAT‑CCCGGGTAAACCAACATCACCGGC‑ATGCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGa                                                    <  2:595697/237‑1 (MQ=255)
                                                                                                                                                                                           aaGTTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGc                                                 <  1:474564/239‑1 (MQ=255)
                                                                                                                                                                                            aGTTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGtca                                                                                                              <  1:173375/177‑1 (MQ=255)
                                                                                                                                                                                              ttATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTg                                                                                                                                                               <  1:478991/127‑1 (MQ=255)
                                                                                                                                                                                              ttAATGGCGGTATT‑CCATAAAGTGAGTGAGGAGCATAT‑CACGGTCAATCCAGCATCACCGGC‑AGTCCGTC‑ATGGCAAGTCCTTAGGCTCAGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTGGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTAc                                                                  <  1:205988/219‑1 (MQ=255)
                                                                                                                                                                                               tATTGGCCGTAGT‑CCATGAAGTGATTGATGAACATAT‑CACGGTCAATCCCACATCACCTGC‑ATTCAGTC‑ATGGCAGGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTGGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAATCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCt                                               <  1:983536/237‑1 (MQ=255)
                                                                                                                                                                                                aTTGGAGGTAGG‑CCAAAAAGTGATTGAGGAGCATAT‑CCCGGTCAATCCATCATCAACGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCtata                                            <  2:772907/239‑1 (MQ=255)
                                                                                                                                                                                                      ggTAGG‑CCATAAAGTGATTGAGGAGCATAT‑CCCGGTCAATCCATCATCCCCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTa                                       <  1:690838/238‑1 (MQ=255)
                                                                                                                                                                                                        tAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTAtt                                     <  1:381680/238‑1 (MQ=255)
                                                                                                                                                                                                           t‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATAACGGATTCAGc                                 >  1:869973/1‑239 (MQ=255)
                                                                                                                                                                                                             ccATAAAGTGTTTGAGGGGCATAT‑CCGGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCa                                <  2:869973/239‑1 (MQ=255)
                                                                                                                                                                                                              cATAAAGTGATTGAGGGGCATAT‑GCCGGTCAATCCATCATCCCCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCa                                <  1:779348/238‑1 (MQ=255)
                                                                                                                                                                                                                     tGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACa                                                      <  1:808298/209‑1 (MQ=255)
                                                                                                                                                                                                                     tGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTa                        >  1:127306/1‑239 (MQ=255)
                                                                                                                                                                                                                       aTTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAACAACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGAATTCAGCAAAATGTTaaa                      >  1:288665/1‑239 (MQ=255)
                                                                                                                                                                                                                       aTTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTTTGCGCACCTACGGTAAAAAAACa                                                      >  2:1027025/1‑207 (MQ=255)
                                                                                                                                                                                                                       aTTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACCAGATGCCTATACCGCATTCAGCAAAATGTTaaa                      >  1:963354/1‑239 (MQ=255)
                                                                                                                                                                                                                       aTTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGCATTCAGCAAAATGTTaaa                      >  1:823326/1‑239 (MQ=255)
                                                                                                                                                                                                                       aTTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGAC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACa                                                      <  1:1027025/207‑1 (MQ=255)
                                                                                                                                                                                                                        ttGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCtcttct                                                                                                                                         >  2:70902/1‑123 (MQ=255)
                                                                                                                                                                                                                        ttGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCtcttct                                                                                                                                         <  1:70902/123‑1 (MQ=255)
                                                                                                                                                                                                                        ttGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTaaaa                     >  1:197757/1‑239 (MQ=255)
                                                                                                                                                                                                                         tGAGGAGCATAT‑CACGGTCAAACCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTaaaaa                    <  1:134698/239‑1 (MQ=255)
                                                                                                                                                                                                                               gCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCTTTCTGGGATGGATGAACGTTGAAAAAACCCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGAATTCAGCAAAATGTAAAAAAAAGAAc              >  2:583535/1‑239 (MQ=255)
                                                                                                                                                                                                                                 atat‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGaaa             >  1:587137/1‑236 (MQ=255)
                                                                                                                                                                                                                                         ggTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGGGCACCTACGGTAAAAAAACAGATGCCTATACCGGATTCGGCAAAATGTTAAAAAGAGGCCATcctcct      >  1:957932/1‑238 (MQ=255)
                                                                                                                                                                                                                                           tCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCCAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATGTTGTGATGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAGTTCATCATTCTGGGATGGATGAACTATGAAAACATTCTGCGCACCTACGTGAAAAACACACATGCCCAAACCGGATTAAGCAAAATGTTAAAAAGAGGaaaacatcatccc   >  2:15125/1‑227 (MQ=255)
                                                                                                                                                                                                                                           tCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AGAGACTGGTCAC‑GGGTCAAATTTGTGGCTGGT‑CGTTATGGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGGCATCATTCTGGAAGTGACGACCGATGAAAAACATCTGCGCACCTCCGGTAAAAAAACAGATGCCTTTAACGTATTCCGAAAAATGTTaaaaaaaaaaaaaacacctacc   >  2:849771/1‑222 (MQ=255)
                                                                                                                                                                                                                                           tCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTACTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAACAAGAGAAAGGCATCATTCTGGGATGGCTGAACAATGAAAAAACTCGGGGCACCTACGGTAAACAAACAAATCCCTATACCGTATTAACAAAAAGGTTAAAAAAAAGACaaaaccatgcc   >  2:19518/1‑229 (MQ=255)
                                                                                                                                                                                                                                               tCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAACAAACAGATGCCTATACCGCATTCAGCAAAATGTTAAAAAGAGGACATCCTCCGGCCg  >  1:793986/1‑236 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                
GGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGT‑TGGGCGCTATATGCTCATCCCTGTTTTC‑AGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCC‑TGAAAATTATC‑AGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGT‑CCATAAAGTGATTGAGGAGCATAT‑CACGGTCAATCCATCATCACCGGC‑ATTCCGTC‑ATGGCAAGTCGTTAGGCTCTGGGAAAAAT‑AAAGACTGGTCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCG  >  NC_000913/3277291‑3277758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑