Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 489,597 T→G 9.5% V1021G (GTG→GGG)  mscK → mechanosensitive channel protein, intermediate conductance, K+ regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913489,5970TG 9.5% 424.1 / 2.1 158V1021G (GTG→GGG) mscKmechanosensitive channel protein, intermediate conductance, K+ regulated
Reads supporting (aligned to +/- strand):  ref base T (59/77);  new base G (14/6);  total (75/83)
Fisher's exact test for biased strand distribution p-value = 3.15e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 159 total aligned reads displayed.

TCGAACGTCCGGTGCGTATTGGCGATACGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTAACCAGCTTGAAGTGCATCTGCACAACGAGAAG  >  NC_000913/489360‑489832
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                            
    aCGTCCGGTGCGTATGGGCGATACGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTa                                                                                                                                                                                                                                          <  1:911421/238‑1 (MQ=255)
            tGCGTTTTGGTGTTACGGTAACCATTGGTAGCTTCTGGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAggc                                                                                                                                                                                                                                  <  2:716265/238‑1 (MQ=255)
                    ggCGATACGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGa                                                                                                                                                                                                                         <  1:494980/239‑1 (MQ=255)
                    ggCGATACCGTAACCAGTGGGAGCCTCTCGGGTACGGTAAGTAATGTCCGTATTCGTGCGACAACGATTACCGATTGCGATCGCAAAGAAGTGATCATCCCTAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGa                                                                                                                                                                                                                         <  1:514434/239‑1 (MQ=255)
                       attatgttAACCTTTGGTAGCTTCTCGGGGACGGTAATTAAAGTCCGTTTGCTGACCACAACGATTACCTGTTTCCATCGCAAGGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCTTCTGGTGATCCGTCTCGGCGTGGCCTCTGGCTCCGATCTGGAAAAAGGGCGTAAAGTGTTACTGAAGGCGGCGACTGAGcc                                                                                                                                                                                                                      <  2:153497/232‑2 (MQ=255)
                        aTACGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTCTTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGCCTGTTACACTGGTCGTTGCCTGACACTACTACGCTCTTGGTGTTCCTTCTCGCCGTGGCCTAGTGCCCCGTCCTGGTAAAATGGGTTAAAGGGTTCCTTAAGGCGGCGAGAGAGCa                                                                                                                                                                                                                      >  2:375816/1‑238 (MQ=255)
                         tatggTAACCGTTTGTGGCTTCTCGGGGGCGGTAAATAAGATACGTGTTCGTGCGAACACGGTTGCCGATTTGGATCGAAAAGAAGTGATCATCCAGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGGCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCAcc                                                                                                                                                                                                                    <  2:168941/236‑1 (MQ=255)
                         atcggtacctttttgTAGCTTTCCGGGGACGGTAAGTAAGAACCGTATTCGTGCGAAAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCAcc                                                                                                                                                                                                                    <  2:935474/225‑1 (MQ=255)
                          aCGGTAACCTTTGGTGGCTTCTCGGGGAAGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACCCTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCAccc                                                                                                                                                                                                                   <  2:727090/239‑1 (MQ=255)
                          aCGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCAccc                                                                                                                                                                                                                   >  1:1043149/1‑239 (MQ=255)
                          aCGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACTGAAGGCGGCGACTGAg                                                                                                                                                                                                                        >  1:956317/1‑234 (MQ=255)
                           cGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCa                                                                                                                                                                                                                  <  1:835864/239‑1 (MQ=255)
                               aaCCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGTGCCTCGGCACCCCAggg                                                                                                                                                                                                             >  1:119275/1‑239 (MQ=255)
                                     tGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGAGTTGGAAAAAGTGCGGAACACGTTACTGACGGCGGGCACGGATAACCCAAGGGGGagg                                                                                                                                                                                                        >  2:75875/1‑236 (MQ=255)
                                      ggTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGCTCTGGAAAAAGGGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCCCCCAAGGGGAATGc                                                                                                                                                                                                       >  1:977777/1‑238 (MQ=255)
                                            ttCTCGGGGACGGTAAGTAAAGTCCTGTTTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGAGCATCCCGAACAAAGGTTTTGGTCCCGAGCGTCTGTTCAACTGGTCGTTGACTGACTCTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAAcc                                                                                                                                                                                                <  2:822407/239‑1 (MQ=255)
                                                 ggggACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGCCTGAGCACCCAAGGGCGATGCACGAACCAATg                                                                                                                                                                                            >  1:902420/1‑238 (MQ=255)
                                                     aCGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGGGCGAAAAGTGTTACTGCAGGCGGCGACTGAGCACCCAAGGGTGAGGCACAACCAAAGGCCGGa                                                                                                                                                                                       >  2:496472/1‑239 (MQ=255)
                                                      cGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGCTCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTGACCTGAACCTACTACGCGGCTGGTGATCCGTCTCGGCGTGGCCAATGGCTCCTCACTGGAAAAAGGGCGTAAAGGGTTACTGAAGGCGGCGAAGGGGCACCCAAGGGTGATCGACGAACCaccacaggaa                                                                                                                                                                                      >  2:892299/1‑230 (MQ=255)
                                                      cGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGGGATGc                                                                                                                                                                                                       >  1:46591/1‑222 (MQ=255)
                                                      cGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACTGAAGGCGGCGCCTGAGCACCCAAGGGTGATGCACGACCCAATGCCGGaa                                                                                                                                                                                      >  1:64445/1‑239 (MQ=255)
                                                           agtaagATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGCCTGGTGATCCGTCTCGGCGTGGCCTATGGCCCCGATCTGGACAAAGGGCGTAAAGGGTTAATGGAGGGGGGGACTGAGCCCCCCAGGGAGATGACAGAACCaagccccgagagcct                                                                                                                                                                                 >  2:234948/1‑226 (MQ=255)
                                                                 aTCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTa                                                                                                                                                                                                                                          <  2:111082/177‑1 (MQ=255)
                                                                 aTCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAAGTCCGGACGTATTCTTTAc                                                                                                                                                                           >  1:657290/1‑239 (MQ=255)
                                                                 aTCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGGGCGTAAAGGGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCAGGAACCAATGCCGGAAgcgtgttttac                                                                                                                                                                           >  1:146983/1‑229 (MQ=255)
                                                                  tCCGTATTCGTGCGACAACGATTACCGATTCCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTa                                                                                                                                                                            <  1:881044/237‑1 (MQ=255)
                                                                   ccGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGCCTGAGCACCCAAGGGGGAGTCACGAACCAATGCCGGAAGCcttctt                                                                                                                                                                              >  1:617761/1‑234 (MQ=255)
                                                                   ccGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGGGATGCACGAACCAATGCCGGAAGGCTTCTTTACg                                                                                                                                                                          >  1:1002012/1‑238 (MQ=255)
                                                                      tATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAggcgg                                                                                                                                                                                                                                <  1:271393/182‑1 (MQ=255)
                                                                      tATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTCACTGAAGGCGGCGACTGGGCACCCAAGGGTGATGCCCGAACCAATCCCGGAAGGGTTTTTTAGGGAAt                                                                                                                                                                      >  2:606575/1‑239 (MQ=255)
                                                                            tGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGGGCGGAAAGGGTTACTGAAGGCGGCGCCTGGGCACCCAAGGGGGGTGCACGAACCAAACCCGGAAGGCTTCTTTACGGGATTggggg                                                                                                                                                                >  2:1038714/1‑237 (MQ=255)
                                                                                aCAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGCGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGGGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAATGTGGTACTGCAGGCGGGGCCTGAACCCCCAACGGGTGTAGCAGGAACCATCAAGGAAGACTTATTTACGGCAAATGGTGCCa                                                                                                                                                             >  2:267029/1‑238 (MQ=255)
                                                                                       ttACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGtt                                                                                                                                                      <  1:75875/238‑1 (MQ=255)
                                                                                       ttACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGGGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTg                                                                                                                                                     >  1:619528/1‑239 (MQ=255)
                                                                                              tttCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTTAGCACCCAAGGGTGAt                                                                                                                                                                                                         >  1:869142/1‑180 (MQ=255)
                                                                                               ttCGATCGCACAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGGGATCCGTCTCGGCGTGGCCTATTGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACTGAGGGCGGGGGCTGAGCACCCAAGGGTGATGCACGAGCCAAGGCCGGAAGCCTTCTTTAAGCCATTTGGTGAAAGCAAGGTgggcaatgg                                                                                                                                             >  2:260708/1‑232 (MQ=255)
                                                                                                tcggtcacaaaaaattgtctatcCCGCACAAGGCTTTTTCTCGCGAGCGTCGGAACGACCGGGCGGGGTCTGACAATACTACGGGTCAGGTGATCCTGCGCGGGGGGGCCGATGGCTAGGATCGGGAAAAAGTGCGTAAAGTGTTGGTGAAGGGGGAGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGCATCATggg                                                                                                                                            <  2:815028/219‑3 (MQ=255)
                                                                                                tCGATCGCAAAGAAGTGATCCTCCCGAACAAAGCGTTTGTTACCGAGCGTCTGAACAACTGGTCGTTGACTGGCACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTTCGTAAAGTTTTCCTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAg                                                                                                                                            <  1:781104/239‑1 (MQ=255)
                                                                                                tCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAg                                                                                                                                            <  1:446224/239‑1 (MQ=255)
                                                                                                 cGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGc                                                                                                                                           <  1:223822/239‑1 (MQ=255)
                                                                                                  gtgcGCAAAGAATTGATCATCCCGAACAAAGCGTTTTTTACCGTGCGTCTGAACAACGGGTCGTTGACTGACACTACGACGCGGCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGGAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCt                                                                                                                                          <  2:902420/236‑1 (MQ=255)
                                                                                                  gacagCAAAGAAGTTATTACCCCGAACGAAGCTTTTGTTTCCGGGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGATTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCt                                                                                                                                          <  2:480893/235‑1 (MQ=255)
                                                                                                  gATCGGAAAGAAGTGATCAGCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCt                                                                                                                                          <  1:428958/239‑1 (MQ=255)
                                                                                                  gATCGGAAAGAAGTGATCAGCCCGAACAAAGCGTTTGGTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCt                                                                                                                                          <  1:234948/239‑1 (MQ=255)
                                                                                                   atgtttataaggtgaagatcCCGAACAAAGCTTTTGTTTCCGAGCGTCTGATCCACTGGTCGTTGCCTGACACGACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCGGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCt                                                                                                                                          <  1:702821/221‑1 (MQ=255)
                                                                                                            aagTGATCCTCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCt                                                                                                                                          <  1:67117/229‑1 (MQ=255)
                                                                                                             attGATCAGCCCGAACAAAGCGTTTGTTTCCGAGGGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGGGGTCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCAACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTa                                                                                                                                 <  1:89987/235‑1 (MQ=255)
                                                                                                                   ctcccctgaCAAAGCTTTTTTTTCCGAGGGCATGATCAGTGGGTCGTTGACTGACCCTACTAGGGGTCTGGTGATCCGGCTGGGGGGGGCCTTTGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTg                                                                                                                         <  2:562179/231‑1 (MQ=255)
                                                                                                                     tcCCGAACAAAGCGTTTGTTCCCGAGCGTCTGATCAACTGGTCGTTGACTGACCCTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAg                                                                                                                                            <  1:25617/218‑1 (MQ=255)
                                                                                                                      cccGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGGGCACCCAAGGGCAATGCACGAACCAATGCCGGAGGTCTTATTTACGGCCTTTGGGGCCACCACGTGGGACAAGCAGCGGGGTCTGTATGTGGGGGaaa                                                                                                                      >  2:452351/1‑238 (MQ=255)
                                                                                                                      cccGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATTTGCGTGAAc                                                                                                                      >  1:953548/1‑239 (MQ=255)
                                                                                                                      cccGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGGGATGCACGACCCAATGCCGGAAGCCTTTTTTACGGCCTTTGGTGCCAGCACGTTGGGCCAGGAGcgccgcctggtgttgctggacc                                                                                                                      >  1:562179/1‑218 (MQ=255)
                                                                                                                      cccGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGGGATCCGTCTCGACGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGCCTTCTTTACGCCATTTGAGGCCAGCACGTTGGATAATGAGCtcggctggaatccggatcctt                                                                                                                      >  2:67117/1‑219 (MQ=255)
                                                                                                                         gAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGCCTCCGATCTGGAAAAAGTGCGCAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGAGGCTGTATGTGCGTGAACTGc                                                                                                                   >  1:301187/1‑239 (MQ=255)
                                                                                                                               aGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCACGGGGGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGACCGTTGGGCCATGAGCTGCGTCTGTATGGGAGTGAACGGCGTGAcc                                                                                                             >  1:294195/1‑239 (MQ=255)
                                                                                                                                  ttttGTTACCGGGGGTGTTGTCAAGTTGTCGTTGACTGACACGACTACGCGTTTGGTTATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCg                                                                                                            <  2:64445/236‑1 (MQ=255)
                                                                                                                                         accggggttCTGATCAGCTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTAc                                                                                                   <  1:1068218/231‑1 (MQ=255)
                                                                                                                                         aCCGGGCGTCGGATCAACTTGTCGTTGACTGACCCTACTTCGCGTCTGGTGAGCCGTCTCGGCGTAGCCTATGGCCCCGATCTGGAAAAAGGGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCCCCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTAc                                                                                                   <  1:361794/239‑1 (MQ=255)
                                                                                                                                                   ttgttaaCTGGTGTTTGGCTGACCCTACTCCGCGTCTGGTGATCCGGCCCGGCTGGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAg                                                                                         <  2:1049699/234‑1 (MQ=255)
                                                                                                                                                   tggtCAACTGGTCGTTGACTGACACTTCTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAg                                                                                         <  1:590374/236‑1 (MQ=255)
                                                                                                                                                   tGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAg                                                                                         <  1:496472/239‑1 (MQ=255)
                                                                                                                                                      ttctttggtcttttatttcctctattaCGGGTTTGGTGTTCCGTCTCGGCGTGGCCTTTGGTGCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGTATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTg                                                                                      <  2:53675/218‑1 (MQ=255)
                                                                                                                                                               cGTTGACTGACACTACTACGCGACTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGGGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGGGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGAGCCCGCACGTCGGATCATGAGATGCGTCTGTATGTGCGTGAACTGCGGGACCGTAGTCGTACTGTCGATGAGCGGAACCggcgt                                                                             >  2:222780/1‑235 (MQ=255)
                                                                                                                                                                  tgactgacCCTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAg                                                                                                                                            <  2:390940/173‑1 (MQ=255)
                                                                                                                                                                  tgactgacACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAg                                                                                                                                            >  1:390940/1‑173 (MQ=255)
                                                                                                                                                                   ggttgacctttttacGCGTTTGGTGTTCCGCTCGGGCGTGGCCTAGTGCTTCGATCTGGAAAAAGTGCGTAAAGGGTTACTGAAGGCGGCGACTTAGCACCTAAGGGTTATGCACGAACCAAGGCCGGAAGTCTTCTTTACGGGATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCg                                                                         <  2:977777/227‑1 (MQ=255)
                                                                                                                                                                    actgacACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa                                                                                                                                                                                                      <  2:90795/113‑1 (MQ=255)
                                                                                                                                                                                    gTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGGGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCCCGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGGAGGCGTACTGGCGATGAGCTGAACCCTCCTACCgcacaccttgaccgtgaa                                                        >  2:242981/1‑222 (MQ=255)
                                                                                                                                                                                      cTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGCTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGGGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCCTTTGGTGCCAGCACGTTGGATCTGGAGCTGCGTCTGGATGTGCGTGAACTCCGTGACCGAAGCCGTACTGTCGGTGGGCTGAACCGTGCTATCGATCAGCTGTGCCGttcaaa                                                      >  2:1043821/1‑234 (MQ=255)
                                                                                                                                                                                      cTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATAAGCTGTGCCGTGaaa                                                       >  1:756219/1‑238 (MQ=255)
                                                                                                                                                                                       tGGGGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAAc                                                     <  1:168249/239‑1 (MQ=255)
                                                                                                                                                                                            aTCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGGGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGGGCTGCGTCTGGATGTGCGTGAACTGCGTGCCCGGAGGCGTACTGTCGATGAGCTGAACCGCACTACTGTTCACCTGTGCCGGGAAAAcaaaac                                                >  2:1078985/1‑234 (MQ=255)
                                                                                                                                                                                                  ctcGGCGTGGCCTATGGCTCCGATCGGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCAAGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACAt                                          <  1:1043821/239‑1 (MQ=255)
                                                                                                                                                                                                   tggggctgggCCTATGGCTCCGTTTTGGAAAAAGGGCGTAAAGTTTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACAt                                          <  2:584032/231‑1 (MQ=255)
                                                                                                                                                                                                   tcGGGGTGGCCTATGGCTCCGAACTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACAt                                          <  1:397951/238‑1 (MQ=255)
                                                                                                                                                                                                   tcGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCt                                                                                                                                          >  2:27120/1‑142 (MQ=255)
                                                                                                                                                                                                   tcGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCt                                                                                                                                          <  1:27120/142‑1 (MQ=255)
                                                                                                                                                                                                   tcGGCGGGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACAt                                          <  1:116127/238‑1 (MQ=255)
                                                                                                                                                                                                    cggggggggcggagggCGCCGAACTGGAAAAAGGGCGGAAAGGGTTACTGAAGGCGGGGCCTGACCACCCAAGGGTGATGCCCGAACCAATGCCGGAAGGCGTCTTGACGGAATTTGGTGCCAGCACGTGGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACAt                                          <  2:713783/224‑1 (MQ=255)
                                                                                                                                                                                                            gCCTATGGCTTCGATCTGGAAAAAGTCCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCGATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTaa                                 <  1:19810/239‑1 (MQ=255)
                                                                                                                                                                                                            gCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGGGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGGGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTAATATCGATCAGCTTGGCCGGGAAAACGAAATCAACAATGCCTTTaa                                 >  2:610340/1‑239 (MQ=255)
                                                                                                                                                                                                             gctgtgggcccGATTTGGAAAAAGTGGGTAAAGGGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTaa                                 <  2:953548/229‑1 (MQ=255)
                                                                                                                                                                                                                     tCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTAACCAGCTTg                         <  1:1078985/238‑1 (MQ=255)
                                                                                                                                                                                                                      ccGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCACCATTGCCTTTAACCAGCTTGaa                       >  1:45336/1‑239 (MQ=255)
                                                                                                                                                                                                                      ccGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTAACCAGCTTGaa                       >  1:796559/1‑239 (MQ=255)
                                                                                                                                                                                                                      ccGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATGGCCTTTAACCAGCTTGaa                       >  1:538818/1‑239 (MQ=255)
                                                                                                                                                                                                                       cGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGTTGAACCGTACTATCg                                                                         >  2:15226/1‑188 (MQ=255)
                                                                                                                                                                                                                       cGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCg                                                                         <  1:15226/188‑1 (MQ=255)
                                                                                                                                                                                                                       cGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGTTCACCTGTGCCGTGAAACCGCAATCAAAATTGCCTTTAACCACCTTGaaa                      >  1:107207/1‑238 (MQ=255)
                                                                                                                                                                                                                       cGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGGACTACCGCTCAGCTGGGCCGGGAAAACCACCTCAACATTGCCTTTAACAAGCTTAAAg                      >  2:813470/1‑239 (MQ=255)
                                                                                                                                                                                                                       cGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGGGCGTGAACTGCGTGACCGTATTCGCACTGTCGATGAGCTGAACCGTGCTATCGATCATCTGGGCCGTGAAAACGACATCAACATTGCCTTTAACCAGCCTGAAg                      >  2:178723/1‑239 (MQ=255)
                                                                                                                                                                                                                       cGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGAATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTAACCAGCTTGAAg                      <  1:178723/239‑1 (MQ=255)
                                                                                                                                                                                                                       cGATCTGGAAAAAGTGCGTAAAGAGTTACTGAAGGCGGCGACTGACACCCACGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGAATGTGCGTGAACTGCGTGACCGTCGTCGTACTGTCGCTGAGCTGAACCGCACTATCGATCAGCTGTGCCGTGAAAACGCACTCAACATTGCCTTTAACAAGCTTGAAGt                     >  2:43126/1‑239 (MQ=255)
                                                                                                                                                                                                                         aTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGTTCAGCTGTGCCGTGAAAACGCCATCAACATTCCCTTTAACCAGCTTGAAgg                     >  2:358636/1‑237 (MQ=255)
                                                                                                                                                                                                                                aaaaGTGCGTAAAGGGTTACGGAAGGCGGCCACTGGGCCCCCAAGGGGTATGGCCGAACCAATGCCGGAAGTCTTTGTTCCGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGAGTCTGTATGTGCGTTAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTAACCAGCTTGAAGTGCATCTGc             <  2:619528/239‑1 (MQ=255)
                                                                                                                                                                                                                                  aaggtctataaGGGTTACTGGAGGTGGGCTATGAGCACCCAAGGGTGATGCACGAACCAAGTCCGGAAGTTTTCTTTACGGTATTTGGTGCCAGCGGGTTGGATCATGAGCTGTGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGCCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTAACCAGCTTGAAGTGCATCTGcac           <  2:77140/230‑1 (MQ=255)
                                                                                                                                                                                                                                  aaGTGCGTAAAGTGTTACGGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTAACCAGCTTGAAGTGCATCTGcac           <  1:768569/239‑1 (MQ=255)
                                                                                                                                                                                                                                        gTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCTGGAAAACGACATAAACATTGCCTTTAACAAGCTTGAAGGGAATcggaaaaagtaa     >  2:100125/1‑228 (MQ=255)
                                                                                                                                                                                                                                          aaaGTGTTACTGAAGGGGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTAACCAGCTTGAAGTGCATCTGCACAACGAGaa   <  1:100125/239‑1 (MQ=255)
                                                                                                                                                                                                                                            aGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTGGTCGTACTGTCGCTGAGCTGAACCGTCGTATCGATCAGCTGTGCCGTGAAAACGACACCAACATTGCCTCTAACACGCTTGAATGGCATCTgtccattataaaa  >  2:83975/1‑226 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                            
TCGAACGTCCGGTGCGTATTGGCGATACGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTAACCAGCTTGAAGTGCATCTGCACAACGAGAAG  >  NC_000913/489360‑489832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: