Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,532,593 C→A 18.1% intergenic (‑156/‑223) yhgE ← / → pck DUF4153 family putative inner membrane protein/phosphoenolpyruvate carboxykinase [ATP]

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,532,5930CA18.1% 207.1 / 4.7 88intergenic (‑156/‑223)yhgE/pckDUF4153 family putative inner membrane protein/phosphoenolpyruvate carboxykinase [ATP]
Reads supporting (aligned to +/- strand):  ref base C (49/21);  new base A (2/16);  total (51/37)
Fisher's exact test for biased strand distribution p-value = 7.13e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGTAGCAAACCAGACCCTGAAGTAATCCGGTAGCAATCATCCCCCAGCGTGTTGCGGGTGAAAGTTCAACGTTATCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTAAATCTATGAGCCTTGTCGCGGTTAACACCCCCAAAAAGACTTTACTATTCAGGCAATACATATTGGCTAAGGAGCAGTGAAATGCGCGTT  >  NC_000913/3532358‑3532824
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                        
agGTAGCAAACCAGACCCTGAAGTAATCCGGTAGCAATCATCCCCCAGCGTGTTGCGGGTGAAAGTTCAACGTTATCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGTGATT‑C‑CA‑CTcac                                                                                                                                                                                                                                          <  1:888872/236‑1 (MQ=255)
  gTAGCAAACCAGACCCTGAAGTAATCCGGTAGCAATCATCCCCCAGCGTGTTGCGGGTGAAAGTTCAACGTTATCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACActa                                                                                                                                                                                                                                      >  1:952355/1‑238 (MQ=255)
             gACCCTGAAGTAATCCGGTAGCAATCATCCCCCAGCGTGTTGCGGGTGAAAGTTCAACGCTATCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGGATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGCAACTTTACCGGTTGAATTTGCATCAATTCCATTC‑AGGAATGCACAT‑C‑CA‑CCCAAAAAATTCCCCCCataa                                                                                                                                                                                                                          >  2:100495/1‑238 (MQ=255)
              aCCCTGAAGTAATCCGGTAGCAATCATCCCCCAGCGTGTTGCGGGTGAAAGTTCAACGTTATCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAG‑ACAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGATTGAATTTGCATCAATTTCATTC‑CGGAATGCGATT‑T‑CA‑CCCACAAAATTCCCGCCaataa                                                                                                                                                                                                                         >  2:203527/1‑235 (MQ=255)
              aCCCTGAAGTAATCCGGTAGCAATCATCCCCCAGCGTGTTGCGGGTGAAAGTTCAACGTTATCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑AA‑CTCACAATATTCCCGCCTtata                                                                                                                                                                                                                         >  1:873918/1‑239 (MQ=255)
              aCCCTGAAGTAATCCGGTAGCAATCATCCCCCAGCGTGTTGCGGGTGAAAGTTCAACGTTATCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTA‑AGGAATGCGATT‑C‑CC‑CTCAAAAAATTCCCCCCataaa                                                                                                                                                                                                                         >  2:872179/1‑237 (MQ=255)
                  tGAAGTAATCCGGTAGCAATCATCCCCCAGGTTGTTGCGGGTAAAATTTCAACTTTATCCATGTTGGTTATCCAGAATCAAAAGGGGGGTAAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAAcc                                                                                                                                                                                                                     <  1:490332/239‑1 (MQ=255)
                    aaGTAATCCGGTAGCAATCATCCCCCAGCGTGTTGCGGGTGACAGTTCAACGTTATCCATGCTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCACTACTTTACCGGTTGAATTTCTATAAATTTCCTTC‑AGGATTGCGATT‑C‑CA‑CCCACAATATTCCCCCCATATAAACCaa                                                                                                                                                                                                                   >  2:612418/1‑239 (MQ=255)
                    aaGTAATCCGGTAGCAATCATCCCCCAGCGTGTTGCGGGTGAAAGTTCAACGTTATCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGAATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCaa                                                                                                                                                                                                                   >  1:773172/1‑239 (MQ=255)
                        aaTCCGGTAGCAATCATCCCCCAGCGTGTTGCGGGTGAAAGTTCAACGTTATCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CCCACAATATTCCCGCCATATAAAACAAAAtt                                                                                                                                                                                                               >  2:99635/1‑239 (MQ=255)
                                                 gttttGGGGGTAAAAGTTAAACGTTACCCATGTGGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAG‑AAAAAAATCAAAAAAAGTTAGCGTGGTAAA‑TCGATACTTACCCGGTTGAATTTCCATCAATTTCATCC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCcac                                                                                                                                                                                       <  1:982480/235‑1 (MQ=255)
                                                 gttttGCGGGGAAAGGTTCAACGTTATCCATTTTGGTTATCCAGAACCAAAAGGTGGTTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCcac                                                                                                                                                                                       <  1:194253/235‑1 (MQ=255)
                                                   gtTGCGGGTGAAAGTTCAACGTTATCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACAcc                                                                                                                                                                                    >  1:350075/1‑239 (MQ=255)
                                                          gcgAAAGTTCAACGTTATCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACAGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATTTAAACCAAGATTTAACCTTTTGAGACAATTTTCCAAACCTAAAATg                                                                                                                                                                             >  2:595944/3‑239 (MQ=255)
                                                                 ttCAACGTTATCCATGTGGGTTACCCAAAACAAAAGGGTGGGTTAATTTTCGAATCGGGGCAGTAGTATTTTGTTTTTTTCAG‑AAAATAATAAAAAAAATTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCAAAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTc                                                                                                                                                                      <  1:384920/239‑1 (MQ=255)
                                                                   aaaCGTTAACCATGTTGGTTACCCAAAATCAAAAGGGGGGTTAATTACCGCATCCGGGCAGTATTATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGAGATT‑C‑AG‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTg                                                                                                                                                                    <  1:648427/238‑1 (MQ=255)
                                                                          aTCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAAATTTAACCTTTTGAGAACATTTTCCCCACCCAAAATCCAATTTTTGCGaaaaa                                                                                                                                                             >  2:363525/1‑238 (MQ=255)
                                                                           tCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGatatta                                                                                                                                                            >  1:191396/1‑239 (MQ=255)
                                                                           tCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGAGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTTGGAACATTTTCCACACCTCAAATGCAAATTTTGCGaaaaaa                                                                                                                                                            >  2:435096/1‑237 (MQ=255)
                                                                                gTTGGTTATCCAGACTCAAAAGGTGGGTTAATTATCGCATCCGGGCAGAAGTATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGCTACTTTACCGGTTGCATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CC‑CTCACAATATTCCCGCCCTATAAACCAAGATTTAACCTTTTGGGAACATTTTTCACACCTAAAATGCTATTTCTGCGATAATAGCac                                                                                                                                                        >  1:837882/1‑237 (MQ=255)
                                                                                gTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTCGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCAAAAATCCAATTTTTGCATTAATAGCaa                                                                                                                                                        >  2:1017709/1‑238 (MQ=255)
                                                                                  tGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCACTTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGACAACATTTTCCACACCTAAAAAGCTATTTCTGCGAAATTAGCAACCg                                                                                                                                                     >  2:101771/1‑239 (MQ=255)
                                                                                     ttATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGaa                                                                                                                                                                                                 >  1:347339/1‑192 (MQ=255)
                                                                                       aTCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTaaa                                                                                                                                                                                >  1:914935/1‑207 (MQ=255)
                                                                                       aTCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTaa                                                                                                                                                                                 >  2:147697/1‑206 (MQ=255)
                                                                                       aTCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTaa                                                                                                                                                                                 <  1:147697/206‑1 (MQ=255)
                                                                                          cAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGGG‑a                                                                                                                                            >  1:826535/1‑239 (MQ=255)
                                                                                             aaTCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAg                                                                                                                                         >  1:767169/1‑239 (MQ=255)
                                                                                             aaTCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAAAATTTTCAACACCTAAAATCAAATTTTGTCGATAATAGAAACCATTTCGTG‑AAAg                                                                                                                                         >  2:497256/1‑239 (MQ=255)
                                                                                              aTCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAgg                                                                                                                                        >  1:928596/1‑239 (MQ=255)
                                                                                               tCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGa                                                                                                                                       >  1:779852/1‑239 (MQ=255)
                                                                                                    aGGTGGTTTAATTACGGCACCGGGGAATTGTTTTTTTTCTTTTTTAAA‑AAAATAACAAAAAAAAGTGAGGTGGGAGAA‑TCGATGCTTTACCGGTTAAATTTGAATCAATTTCATTA‑AGGAATGCGATT‑C‑CA‑CCCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCAc                                                                                                                                  <  1:100495/239‑1 (MQ=255)
                                                                                                     cggggtttAATTATCGCATCCGGGCAGTAGTATTTTGTTTTTTACAG‑AAAATAATAAAAAAAAGTTAGCGGGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATAAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACg                                                                                                                                 <  1:760152/233‑1 (MQ=255)
                                                                                                       tGGGTAAATAATCGAATCGGGGCAGTAGTTTTTTGTTTTTTTAAA‑AAAATAATAAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACgg                                                                                                                                <  1:976905/238‑1 (MQ=255)
                                                                                                       gtggtattttATCACATCGTGGCAGTTGTTTTTTGTTTTTTGAAA‑AAAAAAATAAAAAAAAGTTAGGGGAGAGAA‑TGGATATTTTCCCGGTTGAATTTGCATCATTTTCATGC‑AGTAATGCGATT‑C‑CA‑CTAACAATATCCCCGCCATATAAACCAAGATTTAAACTTTTGAGAACATTTGCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACg                                                                                                                                 <  2:482129/229‑1 (MQ=255)
                                                                                                         ggTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATTTAAACCAAGATTTCACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGCTAATAGCAACCATTT‑GTGTACAGGAATCCCgggat                                                                                                                             >  2:661691/1‑236 (MQ=255)
                                                                                                         ggTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTTCGATAAAAGCAACCGTTTCGTA‑ACAGAAAACACGGAGt                                                                                                                             >  2:976905/1‑239 (MQ=255)
                                                                                                         ggTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGGGATAATAGCAACCGTTTCGTG‑AAAGGaaaaaaggggt                                                                                                                             >  2:87653/1‑230 (MQ=255)
                                                                                                                   cGCATCCGGGCAGTAGTATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCAATTTCTGCGTTAATAGCAACCGTTTCGTG‑ACAGGAAACCAGGAGtttttttttgg                                                                                                                   >  2:760152/1‑234 (MQ=255)
                                                                                                                   cGCATCCGGGCAGTAGTATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGCTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGATAACATTTTccc                                                                                                                                                                                        >  1:601572/1‑170 (MQ=255)
                                                                                                                     cATCCGGGCAGTAGTATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTc                                                                                                                                                                                                                                   >  1:484756/1‑126 (MQ=255)
                                                                                                                      aatggggaagtatatttttGTTTTTTACAG‑AAAATAAAAAAAAAAAGTTAGCGTGGTGAA‑ACGATCCTTTACCGTTTTAATTAGAATCAATGTCATTC‑AGGAAGGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAata                                                                                                                <  1:612418/224‑1 (MQ=255)
                                                                                                                            gCAGTAGTATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAACTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCTAGATTTAACCTTTTGAGAACATTTTCACCACCTAAAATGCTATTTCTGCGATACTAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAAtt                                                                                                          >  1:39989/1‑239 (MQ=255)
                                                                                                                                   ttttttttttttttAAA‑AAAAAAAAAAAAAAAAGTTGGCTGGGAAAA‑TGTATTCTTTACGGGTTAAATCTG‑AACAAATTAAATA‑AAGAATGAACTT‑T‑CACCTAAAAATTTCCCCGCCATATAAACCAAGATTTAACCTTTTGAGACCATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAg                                                                                                   <  2:180655/231‑1 (MQ=255)
                                                                                                                                     ttttGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAACATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGCAATCACGGCGTTTTTTGTCAAATATGAATTTCTCCAGAt                                                                                                 >  1:1018736/1‑239 (MQ=255)
                                                                                                                                      tttGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAACACCGGAGTTTTTTTCTAAATATAAATTTTTCCaaaaa                                                                                                >  2:705922/1‑235 (MQ=255)
                                                                                                                                        tGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACg                                                                                              >  1:180655/1‑239 (MQ=255)
                                                                                                                                          tttttttCAG‑AAAATAATAAAAAAAATTTAGGGTTTTGAA‑CCGATACTTTCCCGTTTAAATTTTCATAAATTTAATTC‑AGGAATGCGATT‑C‑CA‑CTCAAAATTTTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTa                                                                                            <  1:1017709/238‑1 (MQ=255)
                                                                                                                                          cTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAAc                                                                                                                                                                                                                      >  1:971594/1‑118 (MQ=255)
                                                                                                                                          cTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTTTCCAGATACGTa                                                                                            >  1:424550/1‑239 (MQ=255)
                                                                                                                                          cTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTa                                                                                            >  1:207128/1‑239 (MQ=255)
                                                                                                                                          cTTTTTCAAA‑AAAATAATAAAAAAAAGTTGGGGTGGTAAA‑TCAAATCTTTACCGGTTGAATTTGAATAAATTTAATTA‑AGGAATGCGATC‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTa                                                                                            <  1:872179/239‑1 (MQ=255)
                                                                                                                                           ttttttCAA‑AAAATAATAAAAAAAAGTTAGGGGGGTGAA‑TCGATTTTTTACCGGTTAAATTTGCATAAATTTCATTC‑AGGAATGCGATC‑C‑CC‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTa                                                                                            <  1:579177/238‑1 (MQ=255)
                                                                                                                                           ttttttAAA‑AAAATATAAAAAAAATGTTGGCGTGGTAAA‑TCGTTTCTTTA‑CATTTGTATTTACCATAAATTCAATC‑AAGAAACGGTTT‑CTCA‑CTCACAATTTCCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTaa                                                                                           <  2:808210/239‑1 (MQ=255)
                                                                                                                                           ttttttAAA‑AAAAAAAAAAAAAAAAATTTGGGGGGGGAACTCAATTCTTTACC‑GTTTAATTTTGAACAATTTTAATCAAGGATTGCGTTT‑C‑CA‑CTAACATTTTCCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTa                                                                                            <  2:928596/239‑1 (MQ=255)
                                                                                                                                            tttttCAAAAAAATAAAAAAAAAAAGTTACTGGGGAGAA‑TCGATACTTTCCCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCAATT‑C‑CC‑CTACAAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTaa                                                                                           <  1:661691/239‑1 (MQ=255)
                                                                                                                                                tacaaaaaaTAATAAAAAAAAGTTAGCGGGGTGAA‑TCGATACTTTACCTGTTGAAGTTGCAACAATTTCATGC‑AGGAATGCTATT‑C‑CA‑CTCAAAATTTTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTAAATCt                                                                                       <  1:435096/234‑1 (MQ=255)
                                                                                                                                                     aaaaTAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTAAATCTATGa                                                                                   >  1:20433/1‑238 (MQ=255)
                                                                                                                                                       aataatCAAAAAAAGTTGGCGTGGTAAA‑TCGATACTTTCCCGGTTAAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTAAATCTATGAGcc                                                                                <  1:916107/239‑1 (MQ=255)
                                                                                                                                                                         agcggggtaaacgcattcTTTACCGGTTGAATTTGCATCAATTTCATTA‑AGGAATGCCATT‑C‑CC‑CTCAAAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTAAATCTATGAGCCTTGTCGCGGTTAACAcc                                                               <  1:99635/222‑1 (MQ=255)
                                                                                                                                                                          cGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTAAAt                                                                                         >  1:83291/1‑211 (MQ=255)
                                                                                                                                                                          cGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTAAATCTATGAGCCTTGTCGCGGTTAACAccc                                                              >  1:185233/1‑238 (MQ=255)
                                                                                                                                                                              gtgAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTAttt                                                                                                                                                                       >  1:85840/1‑130 (MQ=255)
                                                                                                                                                                              gtgAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTAAATCTATGAGCCTTGTCGCGGTAAAAACCCACaaa                                                         >  2:14955/1‑239 (MQ=255)
                                                                                                                                                                               tgAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTAACTCTATGAGCCTTTGCCCGGTTAAAACCCCCaaaa                                                        >  2:948283/1‑239 (MQ=255)
                                                                                                                                                                               cgAA‑TCGATCTTTTACCGTTTGAATTTGCATAAATTCAATTC‑AGGAATTCGATC‑C‑CA‑CTCAAAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTAAATCTATGAGCCTTGTCGCGGTTAACACCCCCaaa                                                         <  1:30032/237‑1 (MQ=255)
                                                                                                                                                                                        ttttttcccttttttttttGCATTTTTTTCTTAC‑AGTAATGCTATT‑C‑AC‑ATAAAAATTTCCCCCCCATATAAACCAAGATTTAACCTTTTGAAAACATTTCCCACCCCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAACCACGGAGTTTTTTGCCAAATATGAATTTCTCCAGATACGTAAATCTATGAGCCTTGTCGCGGTTAACACCCCCAAAAAGACTTTa                                                <  2:424550/223‑1 (MQ=255)
                                                                                                                                                                                          ttttACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTa                                                                                            <  1:1088322/192‑1 (MQ=255)
                                                                                                                                                                                          cTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTa                                                                                            >  2:1088322/1‑193 (MQ=255)
                                                                                                                                                                                             tccgggTTAAATTTGCATAAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTAAATCTATGAGCCTTGTCGCGGTTAACACCCCCAAAAAGACTTTACTAtt                                           <  1:14955/235‑1 (MQ=255)
                                                                                                                                                                                             tcccGTTTGAATTGGCAACAATATCATTC‑AGGAATGCGATT‑C‑CA‑CTCAAAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTATGTCAAATATGAATTTCTCCAGATACGTAAATCTATGAGCCTTGTCGCGGTTAACACCCCCAAAAAGACTTTACTAtt                                           <  1:948283/237‑1 (MQ=255)
                                                                                                                                                                                              cctggTTTATTTTACATCAATTTATTTC‑AGGAATACCATC‑C‑CA‑CACAAAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTAAATCTATGAGCCTTGTCGCGGTTAACACCCCCAAAAAGACTTTACTATTc                                          <  2:1076091/236‑1 (MQ=255)
                                                                                                                                                                                              cccttttaattttcccttattttCATCA‑AGGAATTCGTTC‑C‑CA‑CTCACAATATTCCCGACATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTAAATCTATGAGCCTTGTCGCGGTTAACACCCCCAAAAAGACTTTACTATTc                                          <  2:888030/219‑1 (MQ=255)
                                                                                                                                                                                              cccGTTTGAATTTACAAAAATTTCATTC‑AGGAATGCAATT‑C‑CA‑CCCAAAATATCCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTAAATCTATGAGCCTTGTCGCGGTTAACACCCCCAAAAAGACTTTACTATTc                                          <  1:497256/238‑1 (MQ=255)
                                                                                                                                                                                                 gtttGAATTTGCATCAATTACATGA‑AGGAAGCGGATT‑C‑CA‑CTAAAAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTAAATCTATGAGCCTTGTCGCGGTTAACACCCCCAAAAAGACTTTACTATTCAgg                                       <  1:638054/237‑1 (MQ=255)
                                                                                                                                                                                                 ggTTGAATTTGCATAAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CCAACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTAAATCTATGAGCCTTGTCGCGGTTAACACCCCCAAAAAGACTTTACTATTCAgg                                       <  1:363525/239‑1 (MQ=255)
                                                                                                                                                                                                  gTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCCCGGTGTTTTTTGTCAAATACGAATTTCTCCAGATACGTAAATCTATAAACCTTGTCGCGGTTAAAACCCCAAAAAAACCTTTAcatttaagga                                      >  1:525310/1‑230 (MQ=255)
                                                                                                                                                                                                  gTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTAAATCTATGAGCCTTGTCGCGGTTAACACCCCCAAAAAGACTTTACTATTCAGGc                                      >  1:888030/1‑239 (MQ=255)
                                                                                                                                                                                                          tgaaaaaaTTTCATTG‑AGAAACG‑GATTCC‑CA‑CTCACATTATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTAAATCTATGAGCCTTGTCGCGGTTAACACCCCCAAAAAGACTTTACTATTCAGGCAATACata                              <  1:994989/233‑1 (MQ=255)
                                                                                                                                                                                                           gCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTAAATCTATGAGCCTTGTCCCGGTTTACACCCCCaaaaaaa                                                     >  1:833945/1‑213 (MQ=255)
                                                                                                                                                                                                            cagcatttacattc‑aTGAGTGTGTTT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATAACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTAAATCTATGAGCCTTGTCGCGGTTAACTCCCCCAAAAAGACTTTTCTATTCAGGCAATACATAtt                            <  1:167737/230‑1 (MQ=255)
                                                                                                                                                                                                             aTCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTAAATCTATGAGCCTTGTCGCGGTTAACACCCCCAAAAAGACTTTACTATTCAGGCAATACATAtt                            >  1:1082948/1‑238 (MQ=255)
                                                                                                                                                                                                                             gAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAGGATTTAACCTTTTGAGAACATTTTCCACACCTCAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGTAATCACGGATGTTTTTGTCAAATATGAATTTCTCCCGGTACGGAAACCTATTAGCGTTTTCACGGTTAACACCCCCAAAAAACCTTTACTATTCAACCAATACACATTGGCGAAGGAGCaagaa            >  2:761206/1‑235 (MQ=255)
                                                                                                                                                                                                                               ttggcttt‑C‑CT‑CTCAATATTTCCCCGCCAATAAAACCAAGATTTAACCTTTTGAGAATATTTTCCACCACTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTAAATCTATGAGCCTTGTCGCGGTTAACACCCCCAAAAAGACTTTACTATTCAGGCAATACATATTGGCTAAGGAGCAGTGaaa          <  2:613849/233‑1 (MQ=255)
                                                                                                                                                                                                                               aggCGATT‑C‑CT‑ACCAAATTATGCCCGCCATATAATCCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTAAATCTATGAGCCTTGTCGCGGTTAACACCCCCAAAAAGACTTTACTATTCAGGCAATACATATTGGCTAAGGAGCAGTGaaa          <  1:670332/237‑1 (MQ=255)
                                                                                                                                                                                                                                     tt‑C‑CA‑CTAAAAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTAAATCTATGAGCCTTGTCGCGGTTAACACCCCCAAAAAGACTTTACTATTCAGGCAATACATATTGGCTAAGGAGCAGTGAAATgcgc     <  1:595944/238‑1 (MQ=255)
                                                                                                                                                                                                                                        c‑cA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGCTAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTAAATCTATGAGCCTTGTCGCGGTTAACACCCCCAAAAAAACTTTACTATTCAGGCAATACATATTGGCTAAGGGACCGTGAAAGGCGCGtt  >  2:47496/1‑239 (MQ=255)
                                                                                                                                                                                                                                             cTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTAAATCTATGAGCCTTGTCGCGGTTAACACCCCCAAAAAGACtt                                                  >  2:10538/1‑188 (MQ=255)
                                                                                                                                                                                                                                             cTCAAAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTAAATCTATGAGCCTTGTCGCGGTTAACACCCCCAAAAAGACtt                                                  <  1:10538/188‑1 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                        
AGGTAGCAAACCAGACCCTGAAGTAATCCGGTAGCAATCATCCCCCAGCGTGTTGCGGGTGAAAGTTCAACGTTATCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAG‑AAAATAATCAAAAAAAGTTAGCGTGGTGAA‑TCGATACTTTACCGGTTGAATTTGCATCAATTTCATTC‑AGGAATGCGATT‑C‑CA‑CTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTG‑ACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTAAATCTATGAGCCTTGTCGCGGTTAACACCCCCAAAAAGACTTTACTATTCAGGCAATACATATTGGCTAAGGAGCAGTGAAATGCGCGTT  >  NC_000913/3532358‑3532824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑