Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,639,574 | A→G | 4.3% | F49S (TTT→TCT) | uspB ← | universal stress (ethanol tolerance) protein B |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,639,574 | 0 | A | G | 4.3% | 311.0 / 3.4 | 93 | F49S (TTT→TCT) | uspB | universal stress (ethanol tolerance) protein B |
Reads supporting (aligned to +/- strand): ref base A (39/50); new base G (2/2); total (41/52) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.94e-01 |
GTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATGCGACGGTGCTTATCATACCTCCTCCCCGGCGACCTGCCCGCGGAGTTCCACCCCGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGG > NC_000913/3639336‑3639806 | gTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGCCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGGCAATAAATTCATCATAGTGATGATCGCGCTAACGTTGGGCCTAGATCTACCAAACGAAACGAACCTGTTTGTTGGGTTGGCCATGAGAGGaaaaaa > 2:85483/1‑239 (MQ=255) tgttgcgccGCTTTTTTTAGGCGTTATTACTGCTGAAGTCACTAATGCCAAATCAACAATTCAATCAGGCGGACCCCCACCAGACCACATAAGGAGCTGGTCAGACTAAGCCGCCGACGCACCCGGTCACAGCGGCGAATAAATTCATCAACGTGATGATCGCGAGAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGcctcc < 2:894991/232‑1 (MQ=255) tccTGGCCCGCTTTTTTTATGCGTTATTAATGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCCCCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCTGCCGATCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGccccc < 1:1094144/237‑1 (MQ=255) ggCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATTCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATTGTGATGATCGCGATAACGTTGGGCAAAAGTATACCAAACAGGAACGACCTGTTTGTTGGGTTGGCCAAGTGAGGCAAAAAAGACccccccc > 2:1008606/1‑238 (MQ=255) cggagttgggtataagagacagttGCTGAAGTGCTTAATGCCAAATCAGCAATGCAATCAGGCTGACCACCACCAGCCCACACAATGCGATGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATc < 1:170274/216‑1 (MQ=255) ccGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCAACCAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATc < 1:458729/238‑1 (MQ=255) cGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATTTAAGGTAAAAAAGCCCCCTCCATCaa > 1:156789/1‑239 (MQ=255) cTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGCACCGCACCTGGTTGTTGGGTTGGCCATGTGAGGTATAAAAGCCCCATCCATCAACa > 1:861526/1‑239 (MQ=255) cTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCCCCATCAACa > 1:223705/1‑239 (MQ=255) tttttttATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACat < 1:56373/239‑1 (MQ=255) ttttcggttttttgtgctacaGTGCTTAATTCCAAATCAAAAATGAAATCAGGTTGCCCACCCCCAGCCCACACAATGCGGTGGACAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGa < 2:391023/219‑1 (MQ=255) ttATGCGTTTTTATTGCTGAAGGGCTTAATGCCAAATAATCAATGCAATCAGGCTGACCCCCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTCTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGa < 2:47355/239‑1 (MQ=255) cGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATaggg > 1:415816/1‑237 (MQ=255) cTGAAGGGCTTAATGCCAAATCAGCAATGCAATCAGGCTGACCACCAACAGACCACAAAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCACCTCCATCAACATATTGATAGAGCAATGGATCg < 1:853349/238‑1 (MQ=255) cTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCCACCGAAAAGCACCTGTTTGTTGGGTTGGCCATGGAGGGTAAAAAAGCCCCCCCCATCAACATATTGa > 2:170274/1‑215 (MQ=255) cTTAATGCCAAAACATCAATGCAATCAGGCTGACCACCACCAGACCCAACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACg < 1:498687/238‑1 (MQ=255) tAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCa < 1:293442/238‑1 (MQ=255) tAAAGCAAAATCCTCAAGGCAAACAGGGTGACCCCCACCGGACCACACAACGCGGTGGTCAGAAAAACCTGCCGGCGCACCCGCTCACAGCGGCGAATAAAGTCATCATCGGGATGATCGCGATAACGTTGGGCAGAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCa < 2:560909/238‑1 (MQ=255) aaTGCCAAATCATTAATGCAATCAGGTGGACCACCACCAGACCAAACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATTATCGGGATAACGTTGGGCATGGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGt < 2:1076106/239‑1 (MQ=255) tGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGcccc > 2:156158/1‑193 (MQ=255) tGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGcccc < 1:156158/193‑1 (MQ=255) aaTCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACCTATTGATAGGGCAATGGATCGCAGTTACGCAGTACAACTa > 1:976749/1‑239 (MQ=255) cacgctccccacccccaGGCCCAACAATGCGCTGGTCAAAAAAAACGGCCGACGCCCCCCGTCACAGGGGGGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtg < 2:415816/231‑1 (MQ=255) gtaccccccccggtccgcccaAGGCTCTGGTCAGAAAAAACGGCCGAGCCACCCGCTCACAGCGGCGAAAAAATTCATCCTCGGGGTGATCGCGATAACGTTGGGCATAGATAGACCAAACGACACGCACCTGTTTGGTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATgag < 2:199661/220‑1 (MQ=255) accaccaccAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATc > 2:70055/1‑173 (MQ=255) accaccaccAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATc < 1:70055/173‑1 (MQ=255) ccaGACCACCAAATGCGCTGGGCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAgcgc < 1:22398/239‑1 (MQ=255) ccaGACCAAACAATGCGTTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAgcgc < 1:448041/239‑1 (MQ=255) gACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACCCCCGTAGGGTGGGGAAAAAGCGCGcc > 1:393023/1‑239 (MQ=255) aCCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATagag < 1:944787/177‑1 (MQ=255) aCCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTCGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTACAAAAGCCCCCTCCATCAACATATTGATagag > 2:944787/1‑177 (MQ=255) aCCACACAAAGCGCTGGGCAGAATAAACTGCCGACCAACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCAt < 1:687601/239‑1 (MQ=255) cacaATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGCCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGGGCAATGGATCGCAGTTACGCAGTACCCCTACCAACCCGCGTAGTGATGAGAAATAGCGCGCCATGtt > 1:315273/1‑239 (MQ=255) acaATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAAcgc < 1:506179/208‑1 (MQ=255) aggcgcTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACa < 1:300725/237‑1 (MQ=255) aTGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATGCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACa < 1:16044/239‑1 (MQ=255) tGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCCCTAACAACGCGCGTAGTGATGAGAAATAACGCGCCATGTTAaaaa > 2:674963/1‑239 (MQ=255) tGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCCCGAAGTGATGAGAAAAAGCGCGCCAGGTTAACaa > 1:997922/1‑239 (MQ=255) tGGTCAGAATAAACTGCCGCCGCCCCCGCTACCAGCGGTGAATAAATTCATCATCGGGATGATGGCGATAACGTTGGGCAGAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCa < 2:787571/238‑1 (MQ=255) aaTAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGaccc > 1:210118/1‑237 (MQ=255) acaatcccgcccccgccgtttacaGGGGCGAATAAATTCAGCATCGTGATGATCGCGATACCGGTGGGCATGGATATACCAAACGGCACGCCACTGATTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCACTCCATCAACATATTGATAGAGCAATGGCTCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATa < 2:805728/218‑1 (MQ=255) aaacccgcgccccacccccctacaGCGGGGAAAAAATTCAACATCGTGATGATCGCGATAACGTTGGGCATAGATAAACCAAACGGGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATa < 2:1054060/218‑1 (MQ=255) cGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAGAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAAcgcgcg < 1:714370/185‑1 (MQ=255) cGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAGAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAAcgcgcg > 2:714370/1‑185 (MQ=255) ccccccctccaGGGGCGAATAAATTCATCATCGTGATGATCGCGATAACGGTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAaataa < 2:614633/230‑1 (MQ=255) cctacaGGGGGGAATAACTTCAACATCGTGATGATCGGGATAACGTTGGACATAGATAGACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATGCGa < 2:156789/236‑1 (MQ=255) cTCACAGGGGGGAATAAACTCACACTTGTGATGTACGCGATAAGGTTGGGCATAGATAGACCAAACGAGACGCCCCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACAGATGGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATTCAAACGACACATAAAGCCCAAAATAATGCGa < 2:806059/239‑1 (MQ=255) cacaggcacgATTAAATTCAACAACGTGATGATCGCGAGAACGTTGGGCATAGAGATACCAAACGAGACGCACCGGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATGCGACg < 2:198250/231‑1 (MQ=255) cacaGCGGCGAATAAATTCATCTGCGTGATGATTGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATGCGACg < 2:223705/239‑1 (MQ=255) tggaaaTAAATTCATCATTGTGAAGAACGCGATAACGTTGGGCATAGAGATACCAAGCGAGACGCACCTGGTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCAGCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATTAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATGCGACGGTg < 2:315273/232‑1 (MQ=255) gcgAATAAATTCATCATCGTGATGATCGCGCTCACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTAAGGTAAAAAAGCCCCCTCCATCACCATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTACCAACGCGCTTAGTGATGAGAAAAAACGCGCCATGTTACCACTACAAACACCCCCAAAAACCCAaaaaaaaacaaaggagcata > 2:573193/1‑225 (MQ=255) gcgAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAg > 2:64685/1‑100 (MQ=255) gcgAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAg < 1:64685/100‑1 (MQ=255) tcatcGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATGCGACGGTGCTTATCATACctcctc > 1:850440/1‑238 (MQ=255) tgatgaTCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGAAGTTACGCAGTACAACTAACAACGCTCGAGGTGATGAGAAAAAAGCCGCCATGTTAACAATCAAAAAGACACAAAAAGCCAAAAAAAATGCGACGGGGCATaaaaaacacccctcccagcg > 2:329279/1‑220 (MQ=255) ttagcttcggATTACGTTGGGGATAAGTATCACAAACGTGACCAACTTGTTTGGTGGGTTGGCCATGGTAAGTAAAAAAGCCCCCTCCAACAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTCCCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATGCGACGGTGCTTATCATACCTCCTCCCCGGCGa < 2:393023/230‑1 (MQ=255) tgatCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACTCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGAAAACGACACATAAAGCCCAAAAAAATGCGACGGTGCTTATCAAACCCCCCCCCCGGCGAca > 2:810050/1‑238 (MQ=255) cgcgATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATGCGACGGTGCTTATCATACCTCCTCCCCGGCGACCTGc > 1:774153/1‑238 (MQ=255) cgcgATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACACGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAACCCAAAATAATGCAACGGGGCTTATCAAACCCCCCCCCCAGCAAccacc > 2:27296/1‑235 (MQ=255) tAACGTTGGGGATTGATTTACCAAACGAGACGCCCCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATGCGACGGTGCTTATCATACCTCCTCCCCGGCGACCTGCCcgcg < 2:210118/238‑1 (MQ=255) tAACGGTGGGCATAAATATCCCAAACGAGACGCCCCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATGCGACGGTGCTTATCATACCTCCTCCCCGGCGACCTGCCcgcg < 1:687384/238‑1 (MQ=255) ggCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAACCCAAAATAATGCGACGGGGCTTATCATACCTCCTCCCCGGCGCCCTGCCCTCGGAGTTccg > 1:223783/1‑237 (MQ=255) aTAGATATACCAAACGAGACGCACCTGTTTGGTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATGCGACGGTGCTTATCATACCTCCTCCCCGGCGACCTGCCCGCGGAGTTCCAccc < 1:1008606/238‑1 (MQ=255) taCCAAACGAGCCGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATGCGACGGTGCTTATCATACCTCCTCCCCGGCGACCTGCCCGCGGAGTTCCACCCCGGGGCTa < 1:329279/239‑1 (MQ=255) taCCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATGCGa > 1:679/1‑181 (MQ=255) taCCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATGCGa < 2:679/181‑1 (MQ=255) aCCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATc > 1:331487/1‑61 (MQ=255) aCCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATc < 2:331487/61‑1 (MQ=255) aaCGAGACGCCCCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATACCGCGCCATGTTAACATTGCAAACGCCACATAAAGCCCAAAATAATGAGACGGTGTTTATCAAACCGCCCACCCGGCGGCTCACCCGCGGAGGTCACCCCCCGGGcaccggc > 2:917176/1‑232 (MQ=255) aaCGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCACCATATTGATAGAGCAATGGATCGCAGTTATGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGCCAACTAAAGCCCAAAAAAATGCGACGGTCCTTATCACACCCCCTCCCCGGCGCCCTCCCCACCGCGACCCACCCCGGCGCAAcccc > 2:608617/1‑236 (MQ=255) aaCGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATGCGACGGTGCTTATCATACCTCCTCCCCGGCGACCTCCCCGCGGAGTTCAACCCCGGGGCAAcgcc > 1:815586/1‑235 (MQ=255) aaCGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGAAAACGACCCATAAAGCCCAAAATAATGCAACGGTGCTTATCATACCTCCTCCCCGCCAACCTTCCCTCGGAATTCCCCCCCgggggaccccc > 2:707296/1‑231 (MQ=255) aCGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAAGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATTCGACGGGGCTTATCAAACCTCCCCCCCGGCGACCGACCCGCGGAGTTCAAACCACGGGATAccacc > 2:469875/1‑235 (MQ=255) cGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATGCGACGGTGCTTATCATAcctc > 1:139922/1‑192 (MQ=255) cGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATGCGACGGTGCTTATCATAcctc < 2:139922/192‑1 (MQ=255) gccccTGTTTGTTGGGTTGGCCATGTGATGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACACCGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATGCGACGGTGCTTATCATACCTCCTCCCCGGCGACCTGCCCGCGGAGTTCCACCCCGGGGCTACCGCTCCCGATa < 1:608617/235‑1 (MQ=255) tttgttGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATa < 2:519708/143‑1 (MQ=255) tttgttGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATa > 1:519708/1‑143 (MQ=255) tttgttGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATGCGACGGTGCTTATCATACCTCCTCCCCGGCGACCTGCCCGCGGAGTTCCACCCCGGGGCTACCGc < 1:356507/223‑1 (MQ=255) tttttggttggCCCTGTGAGGTAAAAAAGGCCCCTCCATCAACATAATGATAGAGAAACGGATCGCAGTTACGCAGTAACACGTACAACGCTCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGACCAAAATAATGCGACGGTTCTTATCATACCTCCTCCCCGGCGACCTGCCCGCGGAGTTCCGCCCCGGGGCTACCGCTCCCGATACGCTGCCAAt < 2:815586/234‑1 (MQ=255) tggCCATGTGAGGTAAAAGAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATGCGACGGTGCTTATCATACCTCCTCCCCGGCGACCTGCCCGCGGAGTTCCAccc < 2:182720/201‑1 (MQ=255) tggCCATGTGAGGTAAAAGAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATGCGACGGTGCTTATCATACCTCCTCCCCGGCGACCTGCCCGCGGAGTTCCAccc > 1:182720/1‑201 (MQ=255) tggCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATTCGACGGTGCTTATCATACCTCCCCCCCGGCGACCTGCCCGCGGAGGTCCACCCCgag > 2:824176/1‑203 (MQ=255) tggCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAACGCAAACGACACATAAAGCCCAAAATAATCCGCCGGCGCTTATCATAACTCCTCCCCGACCACCCCCCCGCGGAGTTCCACCCCCGGCCTCCCGCCCCCCACACGcccgcccctaatacc > 2:356507/1‑224 (MQ=255) gCCATGTTAGGTAAAAAACCCCCCCCCATCAACATATTGATAGAAGAATGGATCGGAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATGCGACGGTGCTTATCATACCTCCTCCCCGGCGACCTGCCCGCGGAGTTCCACCCCGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAaca < 2:14493/238‑1 (MQ=255) cATGTGAGGTTAAAAAGCCCCCATCATAAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACCACGCGCGTAGTGAAGAGAAATAGCGCGACATGTTAACAATGAAAACGACACATGAAGCCCAAAATAATGCGACGGTGCTTATCATACCTCCTCCCCGGCGACCTGCCCGCGGAGTTCCACCCCGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACAcc < 2:223783/238‑1 (MQ=255) gtgAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTaa > 2:277069/1‑108 (MQ=255) gtgAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTaa < 1:277069/108‑1 (MQ=255) tgAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATGCGACGGTGCTTATCATACCTCCTCCCCGGCGACCTGCCCGCGGAGTTCCCCCCCGGGGCCACCGCCCCCCATACGCTGCCAATCAGTTAACACCAGGt > 2:595962/1‑238 (MQ=255) ggTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATGCGACGGTGCTTATCATACCTCCTCCCCGGCGACCTGCCCGCGGAGTTCCACCCCGGGGCTACCGCTCCCGATACGCTGCCAAt < 1:561451/220‑1 (MQ=255) gTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATGCGACg > 1:426217/1‑141 (MQ=255) gTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATGCGACGGTGCTTATCATACCTCCTCCCCGGCGACCTGCCCGCGGAGTTCCACCCCGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTg > 1:14493/1‑238 (MQ=255) gTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATGCGACGGTGCTTATCATACCTCCTCCCCGCCGACCTGCCCGCGGAGTTCCACCCCGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTgg > 1:914610/1‑239 (MQ=255) gTAAAAAAGCCCCCTCCAACAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGTGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATGCGACg < 2:426217/141‑1 (MQ=255) | GTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATGCGACGGTGCTTATCATACCTCCTCCCCGGCGACCTGCCCGCGGAGTTCCACCCCGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGG > NC_000913/3639336‑3639806 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |