Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,749,742	A→G	17.4%	E171G (GAG→GGG)	sgbU →	putative L‑xylulose 5‑phosphate 3‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,749,742	0	A	G	17.4%	156.8 / 2.7	70	E171G (GAG→GGG)	sgbU	putative L‑xylulose 5‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base A (27/28);  new base G (5/9);  total (32/38)
Fisher's exact test for biased strand distribution p-value = 5.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCATAAACTGAACTATCG  >  NC_000913/3749510‑3749976                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                             ttaagagccaggAGTTAAGCTATTCGCCTGGCTCCCGTTCTCGGCATTCGCCCCACCCAGCCTGCAGGTTAGGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCt                                                                                                                                                                                                                                       <  2:504293/228‑1 (MQ=255) cgGGAAATCATGGGTAAAGCGTTTCGCCTGGCGTGCGATCTCGGCATTCGCACCCTCCAGGTGGCAGGTAATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCt                                                                                                                                                                                                                                       <  2:956018/239‑1 (MQ=255)  gggaaaagagtggtaaaagggttCGGCTGGGGCGGGAGTTCGGAAGTCGACCCAGCCAGCTGGCAAGTGTTGACGTCTAATACGAAGGCCACGACGAAGGCCCCCGGCAAAGTTTGGCGGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAG‑GGGACGAG‑ATGCt                                                                                                                                                                                                                                       <  2:245614/217‑1 (MQ=255)        tCATGAGTAAAGCGATTCGCCTGGCGGGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCa                                                                                                                                                                                                                                <  1:92780/239‑1 (MQ=255)        tCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCa                                                                                                                                                                                                                                <  1:1076400/239‑1 (MQ=255)        tCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCa                                                                                                                                                                                                                                <  1:10043/239‑1 (MQ=255)            gAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGt                                                                                                                                                                                                                            <  1:301646/239‑1 (MQ=255)                aaaGCGATTCGGCTGGCGCGCGATCCGGGCATTCGCACCCACCGGGTGGCAGGTTATGGCGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGtt                                                                                                                                                                                                                        <  2:758587/239‑1 (MQ=255)                  caggtttCGCCTGGCGGCGGATCTCGGCTATCCCCCCATCCAGCGGGCAGGTTGTGACGTCTAATACGAAGACCACGACGAAGGCACCCGGCAACGTTGTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGtt                                                                                                                                                                                                                        <  2:112504/232‑1 (MQ=255)                  aGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGGTTATGAAATCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGACCCCCTCACCGGGGTTc                                                                                                                                                                                                                       >  1:454549/1‑238 (MQ=255)                    ctaTTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGGCGAAGGCACACGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCAcc                                                                                                                                                                                                                    <  1:554354/237‑1 (MQ=255)                      aTTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGGGATTATGGAAACCGCGGTTATGAACTCCATCCGCAAAT‑GGAAAAAAT‑GGGACAGG‑TAGCTCGCCCCACCGCGGTTTACCg                                                                                                                                                                                                                   >  1:416810/1‑238 (MQ=255)                       ttCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGGTACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGGG‑ATGCTCGCCTCACCCTGGTTCACCGt                                                                                                                                                                                                                  >  1:758587/1‑238 (MQ=255)                       ttCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCCTTCGCCAAT‑GGGAAAAAT‑GGGGCGGG‑ATGCTCGCCTCACCg                                                                                                                                                                                                                             >  1:504293/1‑227 (MQ=255)                              ggCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGGTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACTAG‑ATGCTCGCCTCACCGGGGTTCACCGTCTACCCgg                                                                                                                                                                                                          >  1:716646/1‑239 (MQ=255)                                gggcggATCTTGGCATTCGCGCCCACCAGCGGGCAGGTTATGAGGTTTATTACGAAGGCCACGGAGAAGGCCCCCGGCAACGTTTGGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGGAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGa                                                                                                                                                                                                         <  2:204087/233‑1 (MQ=255)                                cgcgcgATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGGGATTATGGATACCCCGTTTTTGAACTCCCTCAGCAAAA‑GGAAAAAAT‑GGGGCGGG‑GTGCTCGCCTAACCGTGGTTCACCGTCTaa                                                                                                                                                                                                              >  1:112504/1‑232 (MQ=255)                                              aTTCGCCCCAGCCCGCTGGCAGGTTATGGCGTTTATTACGAAGGCCACGACGAAGGCCCCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCa                                                                                                                                                                                           <  1:506448/238‑1 (MQ=255)                                                 cGCATCTTCCATCTGGCAGGTTATGACGTCTATTACGAAGCCCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATT‑TGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGcc                                                                                                                                                                                      <  1:556917/239‑1 (MQ=255)                                                 cGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCCCCGTCTAACCGGGCGTCGGCAACCCCAgcg                                                                                                                                                                                        >  1:969125/1‑238 (MQ=255)                                                       ctCCAGCTGGCAGGTTATGGCGTTTATTAGGAAGACCCCGAAGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGTATAACAAGTAATGCTGGCGGTGGAGATTAGGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTGTGCCGTCTCCCCGTACGTCGGCAACCTCAGCGCCTgggg                                                                                                                                                                                 <  2:762105/238‑1 (MQ=255)                                                                gCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGGGGAGATTATGGAAACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGGCGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCCACCTCAGCGCCTGGGGGAATTATGt                                                                                                                                                                        >  1:877409/1‑239 (MQ=255)                                                                     ttATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGtt                                                                                                                                                                                                                        <  1:579593/186‑1 (MQ=255)                                                                      ttgggcttttttttcGAAGGCCCCGGCGGAGGCCCCCGGAACCGTTTTGCGGAGGGGCG‑GGCGTGGGACGTGAAACAGGGGGGGGACACAAAAGTAAGGCGGGGGGTGGAAATTAGGGATCCCGGGTTTATGAACTCCATCAGCAAAG‑GGAAAAAAT‑GGGACGAG‑ATGCGCGCCCCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCccgc                                                                                                                                                                  <  2:877409/225‑1 (MQ=255)                                                                              cTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGGAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCCGACGTCCGCAACCTCAGCGCCCGGGGCCATGAGGTTCCCGCCGAACTGa                                                                                                                                                          >  1:1104732/1‑239 (MQ=255)                                                                              cTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGCCGGCAACCCCAGCGCCCGGGGCAATGAAGTTTCCCCCCAACTGa                                                                                                                                                          >  1:125145/1‑239 (MQ=255)                                                                               tATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGGAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGCCGGCAACCTAAACCCCTGGGGCAATGATGTTCCCGccgaaccgaa                                                                                                                                                         >  1:1017102/1‑239 (MQ=255)                                                                                 ttccGAAGCCCCCGCCGAAGGCCCCGGGCAACTTTTTGCGGAGGGGGT‑GGGGGGGGCAGTCGAAAAGGCGGCGGCATCAAAAGTAATGCTGGCGGTGGAGATTAGGGATACCGCGTTTATGAACCCCATCAGAAAAG‑GGAAAAAAG‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTCCCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAAc                                                                                                                                                       <  2:1085662/236‑1 (MQ=255)                                                                                 ttACGAAGGCCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGt                                                                                                                                                                                                                  <  1:755737/180‑1 (MQ=255)                                                                                         aCCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑TGGACGAG‑ATGCTCGCCTCAcc                                                                                                                                                                                                                              >  2:458777/1‑160 (MQ=255)                                                                                         aCCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCAcc                                                                                                                                                                                                                              <  1:458777/160‑1 (MQ=255)                                                                                         aCCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGGTTATGAACTCCCTCAGCAAAT‑GGGAAAAAT‑GGGACGGG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCCGGGGCAATGAGGTTCCCGCCCAACTGaaaacgggacat                                                                                                                                               >  1:164578/1‑230 (MQ=255)                                                                                         aCCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGGCGAACAGGCGGCGGCATCACAAGTAATTCTGGCGGGGGAGATTATCGAGTCCGCGTGTATTACATCCCACCGCAAAT‑GTCAAAAAG‑TGGACGAA‑GTGCCTGACTCACACTCGATGCGCCACAACCCGCAAGTCGGCAACCTCCGCCCCTGGGGCGAGGATGGTCCCCCCGacattgaacggggcctt                                                                                                                                               >  2:772121/1‑223 (MQ=255)                                                                                          ccccgggggagggccccgcggaCGTTTTGGGGGAGGGGTGGGGGTGGGCGGTCGAACAGGCGGTGATAACCAACGGAGTGCTGGGGGTGGAGATTAGGGATACCGCGTTTATGAACTCCATCAGAAAAG‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACGGGGGTTCACCGTCTCCCCGGACGTCGGAATCCTAAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCAtt                                                                                                                                               <  2:547179/218‑1 (MQ=255)                                                                                             cgacgaAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCCCCTGGGGCAATGATGTTCCCGCCGAACTGCAAATGGGCCTTGAcc                                                                                                                                           >  1:959940/1‑239 (MQ=255)                                                                                                  aaGGCCCCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGcc                                                                                                                                                                                      <  1:924851/191‑1 (MQ=255)                                                                                                       ccccGGCAAGTTTTTGCGGAGGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCAAAAGTAAGGCTGGCGGTGGAGATTAGGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATcgccg                                                                                                                                  <  1:875233/237‑1 (MQ=255)                                                                                                            ggccctttttGCTGGGGGGTC‑GGCGGGGGGAGGCGAACGGGGGGCGGCATCACAATTAATGGGGGGGGTGGAGATTATGGATACCGCGTTTATCAACTCGATCATCAAAT‑GGAAAAAGT‑GGGACGGG‑ATGCTCCCCTCACCGTGGTTCACCGTCTCCCCGGAGGTCGGCACCCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATcc                                                                                                                           <  2:292301/233‑1 (MQ=255)                                                                                                                     ttcgcgaggggggtggggggggAGTCGAATGGGCGGGGACCTAACAAGGTAGGCGGGGGGTGGAGATTAGGGGACCCGCGTTTATGAACGCCGTCAGCAAAT‑GGAAAAAAG‑GGGACGGG‑CTGCGCGCCTCCCGGTGGTTCACCGCCTACCCGGACGGCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGaaa                                                                                                                   <  2:783630/221‑1 (MQ=255)                                                                                                                         gagggggt‑ggggtGGGGGTTCTAACGGGGGGCGGCATCACAAGTAGTGCGGGGGGTGGAGATTATGGACACCGCGTTTATGAACTCCATCAGAAAAG‑GGAAAAAGT‑GGGCCGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGaca                                                                                                               <  2:959940/235‑1 (MQ=255)                                                                                                                           aGGGCG‑GGGGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATGATGGATACCGCGTTTATGACCTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGAAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACAcc                                                                                                             <  1:196616/238‑1 (MQ=255)                                                                                                                                        ggCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGGGGGGATTGTGGGTAACGCGGTTATGAAATCCCTCAGGCAAT‑GGGAAAAAT‑GGGGCGAG‑ATGGTCGCCTCACCGTGGTTCACCGTCTCCCCGCACGTAGGCCACCCCCGACCCCGGGGCAATGATGTTCCCCCCGAACCGCAAATGGGGATTGACCCTAACGCCCCC‑AGCCCCCCCAAAAACACCCCACAAGTCACCg                                                                                                >  2:959829/1‑239 (MQ=255)                                                                                                                                             tggAACGGGGGGGGGTATCAAAAGTAAGGGGGGGGGGGGAAATTGGGGACACCGTTTTTAAGACCCCCAGC‑GAAAATGGGAAAAAAG‑GGGACGAG‑AGCCTCGCCTCACCGGGTTTCACCGTCTCCCCGGACGCCGGCAACCTCAGCCCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCa                                                                                            <  2:125145/236‑1 (MQ=255)                                                                                                                                              caaaCGGGCGGGGGCAACACAAGTTTGGCTGGGTGGGGAAATTTTGGAACCCGCGTTTATCAACCCCCTCAG‑AAAT‑GGGAAAAAA‑GGGGCGGGTGTGCCCCCCTCACCGTGGTTCCCCGTCTACCCGGACCTCGGCACCCTCAGCGCCTGGGGCATTGATGTCCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCa                                                                                            <  2:969125/235‑1 (MQ=255)                                                                                                                                                               cacaAGTAATGCTGGCGGTGGAGATTATGGATACCGCGGTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCCCCGCCTGGGGGAATGATGTTCCCGCCGAACTGAAACTGAGCATCGACAGGATCG‑CGCGCATCACCCCTAAAAACACCCCGCCAAGAACCCGGGAAAGTCCCGGAAAGATcgcg                                                                         >  2:113943/1‑236 (MQ=255)                                                                                                                                                                            ggCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGc                                                                                                                                                                                                  <  2:612156/106‑1 (MQ=255)                                                                                                                                                                            ggCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGc                                                                                                                                                                                                  >  1:612156/1‑106 (MQ=255)                                                                                                                                                                                  ggAGATTATGGATCCCGCGTTTTTGCACGCCATCCGAAAAT‑GGAAAAAAT‑GGGCTGAG‑ATGCTCGCCTCCCCGTGGTTCACCGTCTACCCGGACGTCGGCCACCCCAGCGCCTGGGGCAAGGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTAGCGCCGCG‑ATTCCCCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAg                                                      <  2:416810/239‑1 (MQ=255)                                                                                                                                                                                  ggAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCCCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGCATCGCCGCG‑ATCCCCCTGAAAGACACCCAGCCAGGCACCGGGCAAAGTCCCGGACCGTTCCGCGACGTGCCGTTTGGCGAAg                                                      >  1:48481/1‑239 (MQ=255)                                                                                                                                                                                      aTTAGGGATCCCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCg                                                         <  1:1070266/232‑1 (MQ=255)                                                                                                                                                                                           ggATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACCGAAACTGGGCATTGCCCGTATCGCCGCG‑ATCCCCCTGAAAGACACCCAGCCAGCCACCGGGCAAAGCCCCGGCCAGTTCCGCACCGAGCCGTTTGGAGAAgggcgcgcgg                                             >  2:253286/1‑231 (MQ=255)                                                                                                                                                                                             aTACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGGTTGGCGAAGGCTGCGTCGAt                                           >  1:482343/1‑239 (MQ=255)                                                                                                                                                                                               aCCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGa                                                                                  >  1:852122/1‑198 (MQ=255)                                                                                                                                                                                                ccGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCCTTGACCGTATCGCCCCG‑ACCCACCTGAAAGACACCCAGCCCGTCACCGGGACAAGTCCCGGACAGTTCCGCGACGTGCCGGTTGGCGAAGGCTgggcccggg                                          >  2:1070266/1‑229 (MQ=255)                                                                                                                                                                                                 cgcgTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGCCGTGCCGTTTGGCGAAGGCTGCGTCGATTTCg                                       >  1:501321/1‑239 (MQ=255)                                                                                                                                                                                                 ccggTTTAAGAACCCCAACAGCAAAG‑TGAAAAAAG‑GGGACGGG‑ATCCCGCCCTCCCCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATTTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCg                                       <  1:91548/236‑1 (MQ=255)                                                                                                                                                                                                   cgTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGCCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAgg                                                     >  1:824637/1‑223 (MQ=255)                                                                                                                                                                                                             ccccAACAGAAAAT‑GGAAAAAAT‑GGGAAGGT‑GTGCTCGCCTCACCGTGGTTCACGGTCTACCCGGGCGTCGGCAACCTCAGCGCCGGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCa                           <  1:1069035/237‑1 (MQ=255)                                                                                                                                                                                                             cTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCa                           >  1:1105253/1‑239 (MQ=255)                                                                                                                                                                                                                cATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCaaa                         >  1:564771/1‑238 (MQ=255)                                                                                                                                                                                                                 aTCAGCAAAT‑GGAAAAAAT‑GGGCCGGG‑AGGCTCGCCCCCCCGTGGTTCCCCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATTTCCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAAc                       <  1:512456/239‑1 (MQ=255)                                                                                                                                                                                                                   ctccccacatgAAAAAATGGGGACGGA‑CTGCCCCCCCCCCCGGGGTCCGCCCTCCCCCCGGACGTGGGCACCCTCAGCGCTGGGGGCAATGTGGTCCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACCCCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAAc                       <  2:1079421/229‑1 (MQ=255)                                                                                                                                                                                                                      cAAAT‑GGAAAAAAT‑GGGCCGGA‑ATGCCCGCCCCACCGTGGTTCCCCGTCTACCGGGACGGCGGCAACCTCAGCGCCTGGGCAAATGATTTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGc                  <  1:1070445/239‑1 (MQ=255)                                                                                                                                                                                                                        aagagaaaaaaaT‑GGCGCGCG‑GTCCTCGCCTCCCGGTTGTTCCGCGTCTCCCCGGACGGCGGCACCCCCAGCGCCTGGGGCAATGATGCCCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCa                 <  2:454549/233‑1 (MQ=255)                                                                                                                                                                                                                        aaT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCAt                >  1:1091935/1‑239 (MQ=255)                                                                                                                                                                                                                          t‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTCCGTTGGCACCTTCAAAACGCTGCATaa              >  1:532623/1‑239 (MQ=255)                                                                                                                                                                                                                             aaaaaaaG‑GGGACGGA‑ATGCTCGCCTCCCCGTGTTTCCCGGTCTCCCCGGGCGGCGGCACCCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCATaaa             <  1:187870/237‑1 (MQ=255)                                                                                                                                                                                                                                       ggACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCCTAAACTGAACTAt    >  1:449911/1‑238 (MQ=255)                                                                                                                                                                                                                                        gACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCATAAACTGAACTATc   >  1:277048/1‑238 (MQ=255)                                                                                                                                                                                                                                        gACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCCGAACGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCCCGAGGTCCCGGTTGGCGAAGGCCGCGCGGGTTTCGTTGGAATCTTCcaaaacccgcaaaacataaaatatcg  >  2:27405/1‑217 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                             CGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCATAAACTGAACTATCG  >  NC_000913/3749510‑3749976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑