Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,820,324	T→C	15.8%	R282R (CGA→CGG)	ligB ←	DNA ligase, NAD(+)‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,820,324	0	T	C	15.8%	193.6 / 4.0	98	R282R (CGA→CGG)	ligB	DNA ligase, NAD(+)‑dependent
Reads supporting (aligned to +/- strand):  ref base T (35/42);  new base C (16/3);  total (52/46)
Fisher's exact test for biased strand distribution p-value = 3.90e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGCAATATCCCACTCCTGCCAGCGCCT‑GACGGAACCAATATTCAC‑CCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAACGCCCAGAGAATTCAGCGTGTCGCTATCGTCCTGGCGCATCATCAAGCCAG  >  NC_000913/3820088‑3820562                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                ggCGCAATTCCCCCCTCCGCCCAGCGCCT‑GAGGAAACAAATA‑TCACACCGAGGGGCTTTTTTTCGACCCAGACTGCCGGGGGCAGGTGACGAACCCCCCGATATTTGCCCGTCTTTA‑CCCACCGAAAT‑ATGAATTGCGTTC‑ACTGCGGCAACCTGAGCTAAAGGTTG‑ATATTGCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCg                                                                                                                                                                                                                                               <  2:293848/239‑1 (MQ=255)               cgccTGCCAGCGCCT‑GACGGAACCAATATTCAC‑CCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCAt                                                                                                                                                                                                                                <  1:1006306/237‑1 (MQ=255)                tcccgcccccgcCTGGACGGACCCAATTTCCCC‑CCGCTGATCTTTTTTAACCCCCCTCATGACTGGTGGGATTGCCGCAACCACCGATAGTTTACCGCTCTTA‑CCCCCCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCTCTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCAt                                                                                                                                                                                                                                <  2:844275/230‑1 (MQ=255)                tCCTGCCAGCGCCT‑GACGGAACCAATATTCAC‑CCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGAGACGCAACCGCCGATATTTTACCGCTCTTA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCAAATGGCGGGATTTTGCCCAT‑TTCGCCGCACGTACACATA‑CGCAATc                                                                                                                                                                                                                               >  1:734293/1‑239 (MQ=255)                  cTGCCCGCGCCT‑GACGGAACCAATCTACCC‑CCCTTGGCTTTTTTTACCATCCAGAATGACAGGTGCGAGTGACGCACCCCCCGATATCTTACCGCACTTA‑CCCCCCGCAAC‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCgg                                                                                                                                                                                                                             <  2:838404/239‑1 (MQ=255)                   cggagagtggtataagagacagatatTCAC‑CCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCg                                                                                                                                                                                                                              <  1:971618/216‑1 (MQ=255)                    gCCAGCGCCT‑GACGGAACCAATATTCAC‑CCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑CTATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTTGCCGCCCCTACAACTA‑CGCCATCCGt                                                                                                                                                                                                                            >  1:231153/1‑238 (MQ=255)                    gCCAGCGCCT‑GACGGAACCAATATTCAC‑CCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCCCGTACAACTA‑CGCCACCgggg                                                                                                                                                                                                                           >  1:114689/1‑237 (MQ=255)                    gCCAGCGCCT‑GACGGAACCAATATTCAC‑CCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGCCGGGATTCTGGCCCT‑TTCTCCGCCCGTCCAATTA‑CCCCACCgggg                                                                                                                                                                                                                           >  1:595630/1‑237 (MQ=255)                          gcCT‑GACGGAACCAATGTTCAC‑CCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGGTTCTTGCGCT‑TTCGGCCCTCGTACAACTA‑CGCCACAGGTGGCGAAg                                                                                                                                                                                                                     >  1:726026/1‑239 (MQ=255)                          gcCT‑GACGGAACCAATATTCAC‑CCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACg                                                                                                                                                                                                                        <  1:933264/236‑1 (MQ=255)                               gACGGAACCAATATTCAC‑CCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCCCGTACAACTA‑CCCCCTCGGGGACGCAgggga                                                                                                                                                                                                                 >  1:1018536/1‑237 (MQ=255)                                aCGGAACCAATATTCAC‑CCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTTTGGCTCT‑TTTGCCCCCCGTACAACTA‑CGCCCACGGGGACGAAGGGGaa                                                                                                                                                                                                                >  1:599685/1‑239 (MQ=255)                                  ggAACCAATTTTCAC‑CCGCTGCATTTTTTTTCTCCCCAGCATGACGGGCGCGAGTGACGCAACCACCGATATTTACCCGCTTGTA‑CCCCACGCAAA‑CTTAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTCCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAAtt                                                                                                                                                                                                              <  2:305474/239‑1 (MQ=255)                                       cacactttccccccgtGGATTTTTTTAACCGCCAGCGTCACGGGGGGCAGGGACGCCACCCCCGTTATTTTACCGCTTTTA‑CCCACCGCAAT‑CTTAATGGCCTTC‑CCTGCGGCACCCTGGGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCg                                                                                                                                                                                                           <  2:63006/223‑1 (MQ=255)                                          tatTCAC‑CCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACg                                                                                                                                                                                                                        <  1:281269/221‑1 (MQ=255)                                          tatTCAC‑CCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCCCTCGTACAACTA‑CGCCCTCCGTGACGAAGGGTAATTCCGCttt                                                                                                                                                                                                       >  1:140633/1‑238 (MQ=255)                                              cAC‑CCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGTCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGTCGGGATTCTGGCTCT‑TTCGCCGCTCGTTCAACTA‑CGCCACCGGGGACCGAGGGAAATTCCGCTTTcccc                                                                                                                                                                                                   >  1:813065/1‑236 (MQ=255)                                              cAC‑CCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCCCT‑TTCCCCGCCCGGACAACAA‑CGCCCACCGGGCCCAAGGGGAAATCCGCCTTccac                                                                                                                                                                                                   >  2:292860/1‑238 (MQ=255)                                              cAC‑CCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGCGATTCTGGCCCT‑TTCGCCCCCTGTCCCACTA‑AGCCCACCGGGGCGCAGGGGAATTTCCCTTTccccc                                                                                                                                                                                                  >  2:720931/1‑239 (MQ=255)                                                gcggacgcgtcaggttttTCATACAACACGACGGGTGGGGTTGTCGCAACCCCCCTTTTTTTCCCCCTCTTA‑CCCCCCCAAAA‑CTGAATTCCCTTC‑CCTTGGGCAACCTGGGCTCCAGGTTG‑ATATTCCCAGGCCCCCAGCCCCTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTccacca                                                                                                                                                                                                 <  2:35662/225‑1 (MQ=255)                                                  ccGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCCCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGGGACGCAGGGGAATTCCGCTTTCcaccac                                                                                                                                                                                                >  1:1052999/1‑238 (MQ=255)                                                     cTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGAGTCTGGGCCT‑TTTCCCGCTCGTCCAACAA‑CCCCCCCGGCGAGGAAGGGGAATTccctctccttttaaaac                                                                                                                                                                                             >  2:281269/1‑223 (MQ=255)                                                                     tcCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGCAGCCAATGGCGGGATTCATGCTCT‑TTCACCGCCCGTACAACCACCGCCA‑AGGTGACGAAGGGGAATTCCCGTTTccccccaccatttcggaccccgccaa                                                                                                                                                                             >  1:10841/1‑217 (MQ=255)                                                                       cAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑AGCCATCGGTGACGAAGGGTAATTCCGCTTTCCCCCACCCATTGCGTACGCGCGCacat                                                                                                                                                                           >  1:942778/1‑235 (MQ=255)                                                                          cATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCCCT‑TTTCCCCCCCCTACCACTA‑AGCCAACGGGGGCGAATGGGAATTCCCCTTTCCCCCACACAtctcgtcccccccccaaccaca                                                                                                                                                                       >  2:353324/1‑218 (MQ=255)                                                                          cATCACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTA‑CCCCCCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCGGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGGGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTtcat                                                                                                                                                                       <  1:399689/239‑1 (MQ=255)                                                                             taCAGGGGCGAGTGACGCAACCACCGATATTTTACCGCTCTTA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCCCCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACt                                                                                                                                                                                               <  1:653804/211‑1 (MQ=255)                                                                               cAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCTTCAGCa                                                                                                                                                                  >  1:161530/1‑239 (MQ=255)                                                                                    gCGAGTGACGCAACCACCGATATTTTACCGCTCTTA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCCCC‑TTCGCCGCCCCTACCACTA‑CGCCCCCGGTGACCAAGGGTGATTCCGCTTTCCACCCCCCATTTGGTACGCGCGCAAACTTATCCGCAtttt                                                                                                                                                              >  1:327706/1‑238 (MQ=255)                                                                                       aGTGACGCAACCACCGATATTTTACCGCTCTTA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGTCGCAGGGTGATTCCGCTTTCCACCACTCATATCGGAAGCGCGCAGCTTCTTCAGCCCTTTTCa                                                                                                                                                           >  1:725432/1‑238 (MQ=255)                                                                                        gTGACGCAACCACCGATATTTTACCGCTCTTA‑CCCACCGGAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCAc                                                                                                                                                          <  1:353324/238‑1 (MQ=255)                                                                                        gTGACGCAACCACCGATATTTTACCGCTCTTA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCCCGTACAACTA‑CGCCCTCGGTGACGAAGGGGAATTCCGCTTTCCACCCCCCATTTCCTCACCGCCCAACTTCATTCGCAatttttcc                                                                                                                                                          >  1:23003/1‑235 (MQ=255)                                                                                         tGACGCAACCACCGATATTTTACCGCTCTTA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCC‑TTCGCCGCTCGTACAACTA‑CGCCATCGGGGGCGCAGGGGAATTCCGCTTTTCACCACTCACTggga                                                                                                                                                                                       >  1:35662/1‑205 (MQ=255)                                                                                          tcccccacccccGTTATTTTCCCCCTCTTC‑CCCCCCGCAAA‑CGGAATTGCCTCC‑CCTCGCGACGCCGGGGTCACAGGTTG‑AGTTTCCCAGGCCCCCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGc                                                                                                                                                       <  2:1027401/233‑1 (MQ=255)                                                                                          gccccaaccaacGATTTTTTCCCCCTTTGT‑CCCACCAGAAA‑CTGAAATGCCCTC‑CGTTCAGCAACCGGACCTACAGGGTG‑ATGTTTCCAGGCCCCCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGc                                                                                                                                                       <  2:1028547/235‑1 (MQ=255)                                                                                          gACGCAACCACCGATATTTTACCGCTCTTA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGc                                                                                                                                                       <  1:163683/239‑1 (MQ=255)                                                                                           aCGAAACCCCCGATATTTTCCCGCTCTTA‑CCCACCGCAAA‑CTGAATGGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCa                                                                                                                                                      <  1:258416/239‑1 (MQ=255)                                                                                            ccacacccccatttttttTCCGCTCTTC‑CCCCCCTCAAA‑CTGAATTGCCTTC‑CCTTCGGAAACCTGGGTCAAAGGTTG‑ATTTTTCCAGGCCCCCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCAc                                                                                                                                                     <  2:1018536/225‑1 (MQ=255)                                                                                            cGCAACCACCGATATTTTACCGCTCTTA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCCTCGGTGACGAAGGGTAATTCCGCTTTCCACCACCCATTTCGTAACCGCGCAACTTCATCAGCATTTTTTACAGCAc                                                                                                                                                     >  1:711764/1‑239 (MQ=255)                                                                                            aagcagccccGATATTTTCCCGCCTTTA‑CCCCCCGAAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCAc                                                                                                                                                     <  1:468936/231‑1 (MQ=255)                                                                                             gCAACCACCGATATTTTCCCGCTCTTC‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCAc                                                                                                                                                     <  1:991160/238‑1 (MQ=255)                                                                                             cccccccaccatgatttcaccctctTT‑CCCCCCCCAAACCTTAATTGCCCTC‑CCCCCGACACCCTGGTCT‑CAGGGTGTATATTCCCGGGCCAACAGCCACGCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TGCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCAc                                                                                                                                                     <  2:23003/218‑1 (MQ=255)                                                                                                   cccccttttttTCCCCCCTTACCCCCCCCCAAA‑CGGAATTCCCTTC‑CCTTCGGCCACCTGGACTACAGGTGT‑ATTTTTCCAGGCCGCCCGCCACTCCGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGGGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGtat                                                                                                                                               <  2:942778/232‑1 (MQ=255)                                                                                                    acgttatTTTCCCGGTCTTC‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTTCAGGTTG‑ATATTTCCAGGCCCCCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATAc                                                                                                                                             <  1:569808/235‑1 (MQ=255)                                                                                                       atatTTTACCGCTCTTA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTc                                                                                                                                          <  1:796426/239‑1 (MQ=255)                                                                                                             tACCGCTCTTA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCCCT‑TTCCCCCCCCGTACAACTA‑CGCCATCCGTGACGAAGGGTAATTCCGCCTTCCACCACTCATTTCCTACCCGCGCCACTTCATCAGCATTTTTCACCGCCCGGGTCTAccccctctc                                                                                                                                     >  2:468936/1‑230 (MQ=255)                                                                                                                  ctctTA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTTAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCCCCCCGGGATACGCTTGCGtttaaga                                                                                                                               >  1:536599/1‑234 (MQ=255)                                                                                                                  ctctTA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGCAGGGTAATTCCGCTTTCCACCACTCATTGCGTACCCGCCCAAATTCATCAGCATTTTTCACCGCACGGGGAAACGTCTGCGTTAAAGt                                                                                                                               >  1:757634/1‑239 (MQ=255)                                                                                                                   ctctcccccctcgaaaa‑CTAAATTGCCTCC‑CTCTGGGCACCCGTGGCTACGGGTTT‑ATTTTTCCAGGCCCCCCGCCCCTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGt                                                                                                                               <  2:959878/225‑1 (MQ=255)                                                                                                                     ttA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACg                                                                                                                                                                                                                        <  1:403143/147‑1 (MQ=255)                                                                                                                     ttA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAAATA‑CGCCATCGGTGACGAAGGGGAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCCTCAGCATTTTTCACCGCACGGGGATACGTCTGCGTTAGAGTaaa                                                                                                                            >  1:153941/1‑239 (MQ=255)                                                                                                                        ccccACCCCAAA‑CTGAATGTCCTCC‑CCTACGGCAACCGGAGGACCAGGTTG‑TTGTTACCAGGCCCCCAGCCACTCTCCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAAc                                                                                                                          <  2:146210/238‑1 (MQ=255)                                                                                                                           ccccGCAAA‑C‑GTATTGTCTTCTTCTGCGGCTCCCTGAGCTACGGGTTG‑ATTTTCCCGGGCCACCAGCCACTCTGCCGGGCCCGGTAGCCAATGGGGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATGTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAACCTg                                                                                                                       <  2:153941/237‑1 (MQ=255)                                                                                                                             ccGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑AGCCAACGGGGGCGAAGGGTGATTCCCCTTTCCACCCCCCCTTGCGACCCCGCCCAACTTCATCAGCCTTTTTTACCCCCAGGGGATAAGTATAGCTTAGAGTCAAAcaagcg                                                                                                                     >  2:728355/1‑234 (MQ=255)                                                                                                                              cGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCCCAACTTCATCAGCATTTTTCACCCCACGGGTATACGTCGCCGGTCAAGTAAAACCTGCgg                                                                                                                    >  1:1047193/1‑239 (MQ=255)                                                                                                                              cGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCCGTGACGAAGGGGAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCCCCCCACCGGTATACGTCTGCGTTTGAGTAAAAACTCCgg                                                                                                                    >  1:81906/1‑239 (MQ=255)                                                                                                                              cGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCCCGTACAACTA‑CGCCCTCGGGGACCCAAGGCAATTTCCCTTTCCACCACTCACTGCGCACGCCCGCCACTTCCCCAGCATTTTTTACCCCCCCGGTAATCTTCTCCGTTTGCGTAAAACACGCgg                                                                                                                    >  2:903906/1‑239 (MQ=255)                                                                                                                                cAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGGATAc                                                                                                                                             >  1:812575/1‑212 (MQ=255)                                                                                                                                      tGAATTGCCTTC‑ACTTCGGCACCCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAACCTGCGGTAGCCAg                                                                                                             <  1:728355/239‑1 (MQ=255)                                                                                                                                         aTTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGGAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTTACCGCACGGGTATACGTCTTCGTTagaggataa                                                                                                                           >  1:848933/1‑217 (MQ=255)                                                                                                                                                   aCTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGGAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCCCTTTTAAACg                                                                                                 >  1:727187/1‑239 (MQ=255)                                                                                                                                                     ttcccccccctggccccagggTTG‑ATTTTCCCAGGCCCCCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGAt                                                                                               <  2:727187/220‑1 (MQ=255)                                                                                                                                                        ggCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCCGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGGCGAAGGGTAATTCCGCTTTCCCCAACTCACTGAGCACACGCGCAACTTCATCACACTTTTTTCCCCCACGGGTCAACGCCTCTGTTAGAGTAAAACCTACGGTAGCAACACCTTTTaaaacacaca                                                                                            >  2:550068/1‑232 (MQ=255)                                                                                                                                                            ccctggcatACAGGTTG‑ATTTCTCCGGGCCCCCCGCCTCTCTGCCTGGCCCGGTGTCCAATGGCGGGATTCGGGCTCC‑TTCGCCGCCCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACGCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACAt                                                                                        <  2:231153/231‑1 (MQ=255)                                                                                                                                                               tGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCCCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCCTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAAAGTAAAACCTCCGGTAGCCAGCTCTTTTTAACGATCCGACATTaa                                                                                     >  1:207862/1‑239 (MQ=255)                                                                                                                                                                 aGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTtaa                                                                                                   >  1:523077/1‑223 (MQ=255)                                                                                                                                                                 aGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGAGTCTGGCCCT‑TTCGCCGCTCCGACAACTACCGCCA‑CCGTGACGGAGGGTAATTTCCCTTTCCACCCCTCCCTTCCTACTCCCGCCACTTCATCAACATTTTTCACCGCCCCGGCTTACGTCTGCGTTTGAGATAAACCGTCGGTAGCCACCCCCTTTAAACGATCCAACCTTAAca                                                                                   >  2:441525/1‑238 (MQ=255)                                                                                                                                                                 aGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGACTCTGGCTCT‑TTTGCCGCCCCTACAACTA‑CGCCCTCGGTGCCGAAGGGGAACTCCGCCTTCCACCACTCATTGCGAACGCGCGCCACTTCATCAGCAATTTTTACCGCACGGGTTTACGTCTGCCTTACAGGAAAAACTGCGGATCCCAACTCTTTTAAACACACAAACATTaaaa                                                                                   >  2:405461/1‑237 (MQ=255)                                                                                                                                                                           tG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCCCGTACAACTA‑CGCCATCGGGGACGACGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTTGAGTAAAACCTGCGGGAGCCAGCCCCTTTTAACGATCAGACATTAACTGCGGTCCAt                                                                          >  1:944911/1‑238 (MQ=255)                                                                                                                                                                           tG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCCCT‑TTCGCCGCTCGGCCCACTA‑CCCCCCCGGGGACGAAGGGTAATTTCGCTTTCCCACACCCATTGCGCACCAGCGCAACTTCCTCAGCATTTTTCCCCCCCCGGGTATACGACTTCGTTAAGATAAAACCCTGGGGAACCCGCCCTTTTTAACGAAAAGGAATTAACTGGGGTcccc                                                                          >  2:468558/1‑236 (MQ=255)                                                                                                                                                                              atatTTCCAGGCCCCCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTtcat                                                                                                                                                                       <  1:218937/143‑1 (MQ=255)                                                                                                                                                                                        gCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCCCACGGGGATACGTCTGCGTTAGAGTAAAAACTGCGGTAGCCAGCCCCTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATTCCCa                                                             >  1:188934/1‑239 (MQ=255)                                                                                                                                                                                             caGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGCGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGGATACGTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGCTCAGACATTAACTGCGGTCCATCCGCCCACGCCCAGACaa                                                        >  1:416837/1‑239 (MQ=255)                                                                                                                                                                                              aGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACAtt                                                                                       <  1:938667/207‑1 (MQ=255)                                                                                                                                                                                                       gCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGGAATTCCGCTTTCCAACACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCCGACAAAAAACCCCac                                              >  1:902412/1‑238 (MQ=255)                                                                                                                                                                                                        tcgtgCCCGGTGAGCAAGGGCGGGATTGTGG‑TCTCTTCGGCGGCCTGACAACTC‑CGCCATCCGTGAACAAGGGTAATTCCGCTTTCCACCCATCATTGCGTACGCGCGCAACTTCATCAGCAGTTTTCACCGCACGGGTAAACGTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCGTGTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAACGCCCaga                                             <  2:759441/235‑1 (MQ=255)                                                                                                                                                                                                         cTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAACCGCCCaga                                             <  1:716018/238‑1 (MQ=255)                                                                                                                                                                                                              ccGGTCGCCAGTGGCGGGATTCTTGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCCCCCCTCATTGCGTGCGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAACGCCCAGAGAAtt                                        <  2:188934/238‑1 (MQ=255)                                                                                                                                                                                                                ggTAGCCAATGTGTGGATTCGGGCTCT‑TGCGCCGCTCCTACAACTA‑CGCCATCGGTCACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAACGCCCAGAGAATTCAg                                     <  2:902412/239‑1 (MQ=255)                                                                                                                                                                                                                ggTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCGCGAACAACTA‑CGGCCTCTGTGACTAAGGGGAATTCCGCTTGCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAACGCCCAGAGAATTCAg                                     <  1:476984/239‑1 (MQ=255)                                                                                                                                                                                                                 gTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGGGACGAAGGGGAATTCCGCTTTCCACCACCCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACACGGATACGTCTGCGTTTGAGGAAAACCTTCTGTAGCCCACTCTTTTAAACGACCAGAAATTTACTCACGTTCACCCCGCCATGCCCCCCCAAAAAAGccacaaaaagtaaca                                    >  2:292310/1‑226 (MQ=255)                                                                                                                                                                                                                  tAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAACGCCCAGAGAATTCAGCg                                   >  1:418150/1‑239 (MQ=255)                                                                                                                                                                                                                  tAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCCCTTTTAAACGGTCAGAACTTAACTGCGGTCCATCCGGCCATGCCCCACAAAAAACGCCCAGAAAATTCAGCg                                   >  1:959623/1‑239 (MQ=255)                                                                                                                                                                                                                    gCCAATTGCGGGATTCTGGCTCT‑TGCGCCGTCCGTACAACTA‑CGCCATCGGTGACTAAGGGTAATTCCGCTTTCCCCCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAACGCCCAGAGAATTCAGCgt                                  <  1:17240/238‑1 (MQ=255)                                                                                                                                                                                                                      cAATGGGCGGTTTTTGGCCTT‑TTCCCCCCTCGTCAAAATA‑CGGCATCGGGGACGAAGGGTAATTCCGCTTTCCCCCACTTATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAACGCCCAGAGAATTCAGCgtgt                                <  2:1047193/238‑1 (MQ=255)                                                                                                                                                                                                                      cAATGGCGGGATTCTGGCTCT‑TTCGCCCGTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAACGCCCAGAGAATTCAGCgtgt                                <  1:292310/238‑1 (MQ=255)                                                                                                                                                                                                                          ggCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGGGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGGATACGTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCCTGCCCCGACAAAAACGCCCAGAGAATTCAGCGTGTCGCTa                           >  1:884856/1‑239 (MQ=255)                                                                                                                                                                                                                           gCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAACGCCCAGAGAATTCAGCGTGTCGCTa                           <  1:434522/238‑1 (MQ=255)                                                                                                                                                                                                                            cGGGATTTTTGCTCT‑TTCGCCGCTCGCACAACTA‑CGCCCTCGGTGACGAAGGGTAATCCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAACGCCCAGAGAATTCAGCGTGTCGCTAt                          <  2:140633/238‑1 (MQ=255)                                                                                                                                                                                                                              ggATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAACGCCCAGAGAATTCAGCGTGTCGCTATCg                        >  1:184422/1‑238 (MQ=255)                                                                                                                                                                                                                                    tGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGGGGCGGAGGGGAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCCCGGGGATACGGCTGCGTTGAAGTAAAAACTACGGTAGCCAGCCCCTTTTAACGATCCAACATTAAATGCGGTCCCTCCTGCCATGCCCCGACAAAAAAACCCACAAACATCACCGGGTCCCAACCGTCCCGgc                 >  2:537699/1‑239 (MQ=255)                                                                                                                                                                                                                                     ggCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGAACTTAACTGCGGTCCATCCGGCAATCCCCCGACAAAAACCCCCAAAAAATTCAGCGGGTccccacctccccggcg                >  2:825316/1‑224 (MQ=255)                                                                                                                                                                                                                                       ctct‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTCACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAACGCCCAGAGAATTCAGCGTGTCGCTATCGTCCTGgcgc               <  1:550068/238‑1 (MQ=255)                                                                                                                                                                                                                                            ttCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAACACCCAGAGAATTCAGCGTGTCGCTATCGTCCTGGCGcatca           >  1:337848/1‑238 (MQ=255)                                                                                                                                                                                                                                             tctccgcTCCTAAAACTA‑CCCCAACGGTGCCGAGGGGTCATTCAGGCTTCCACCACTCATTGCGTACGCTGGCAACGGCCTCAGGATTTTTCACCGCACGGGTATACGGCCGCGTTAGAGTAAAACCGGCGGTAGCCAGCTCTTTTAAACGATCAGGCATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAACGCACAGAGAATTCAGCGTGTCGCTATCGTCCTGGCGCatcatc         <  2:899669/236‑1 (MQ=255)                                                                                                                                                                                                                                                   cTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAACCTGCGGTAGCCAg                                                                                                             <  1:851383/133‑1 (MQ=255)                                                                                                                                                                                                                                                   cTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAACCTGCGGTAGCCAg                                                                                                             >  2:851383/1‑133 (MQ=255)                                                                                                                                                                                                                                                    tCGTACAACTA‑CGCCAGCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAACGCCCAGAGAATTCAGCGTGTCGCTATCGTCCTGGCGCATCATCAAGCCAg  <  1:317816/239‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                GGCGCAATATCCCACTCCTGCCAGCGCCT‑GACGGAACCAATATTCAC‑CCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTA‑CCCACCGCAAA‑CTGAATTGCCTTC‑ACTTCGGCAACCTGAGCTACAGGTTG‑ATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCT‑TTCGCCGCTCGTACAACTA‑CGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAACGCCCAGAGAATTCAGCGTGTCGCTATCGTCCTGGCGCATCATCAAGCCAG  >  NC_000913/3820088‑3820562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑