BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,897,085	T→G	8.9%	C105G (TGT→GGT)	yieH →	phosphoenolpyruvate and 6‑phosphogluconate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,897,085	0	T	G	8.9%	439.4 / 3.1	161	C105G (TGT→GGT)	yieH	phosphoenolpyruvate and 6‑phosphogluconate phosphatase
Reads supporting (aligned to +/- strand): ref base T (70/71); new base G (15/2); total (88/73)
Fisher's exact test for biased strand distribution p-value = 3.48e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 168 total aligned reads displayed.

TGTAACGATGTTTCAGGAATTTGGTATTACGCTCGATCCTGAAGAGGTATTCAAACGTTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCGGCGTTAATGTTCCATGCGGCAAAAGCGATGAATGTAAATGTAGAAAACTGCATTCTGGTTGATGACTCAGTTGCCGGTGCACAATCTGGTATCGACGCAGGTATGGAAG  >  NC_000913/3896850‑3897316

tgaaaCGATGGTGCAGGAATTTGGGAGTACGGTCGATCCTGAAGAGGTAGTCAAACGGTGCAAAGTTGTAATACTGTACGAAATTATCGATATTGTTTCCGTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGGTTGCCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg                                                                                                                                                                                                                                       <  1:579075/236‑1 (MQ=255)

tGTAACGATTTTTCAGGAATTTGGTATTACGCTCGATCCTGAAGGGGTATTCAAACGTTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg                                                                                                                                                                                                                                       <  2:796447/239‑1 (MQ=255)

tGTAACGATGTTTCAGGAATTTGGTATTACGCTCGATCCTGAAGAGGTATTCAAACGTTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg                                                                                                                                                                                                                                       <  1:832926/239‑1 (MQ=255)

   aaCGATGTTTCAGGAATTTGGTATTACGCTCGATCCTGAAGAGGTATTCAAACGTTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGAGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCCCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTgg‑gg                                                                                                                                                                                                                                   >  1:294427/1‑239 (MQ=255)

                      ggTATTACGCTCGATCCTGAAGAGGTATTCAAACGTTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATaa                                                                                                                                                                                                                <  1:248419/239‑1 (MQ=255)

                           tACGCTCGATCCTGAAGAGGTATTCAAACGTTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGGTCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACaaaa                                                                                                                                                                                                           >  1:407901/1‑239 (MQ=255)

                                  gATCCTGAAGAGGTATTCAAACGTTTCACAGGTGTAAAACTGTACGAAATTATAGATATTGTTTCCCTTGAACATGGGGTTAAGGTAGCGAAAACAGAAGCTGAACACGATTACCGGGCAGAAGTCGCTCGGCTGTTCGATTCTGACTTGGAAGCCCTCGACAGGGCTGGAGCACGCCTGCAATCGACCACTGCGCCAATGGGGGA‑GGATTTAAAAGGACCAAAAAACaaaaaggagat                                                                                                                                                                                                    >  2:942155/1‑232 (MQ=255)

                                  gATCCTGAAGAGGTATTCAAACGTTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTTCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATgcagca                                                                                                                                                                                                    >  1:802401/1‑239 (MQ=255)

                                        gAAGAGGGATTCAAGCGTTTCAAAGGGGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGCAGCTCGGCTGTTCGGTTCAGACCGGGAAGCCAGCAAAGGGGCTGGAGCCCTCCGGCCCGCGTTCCCTGCGCCAATATGGGT‑GGGAGCTAACGGCCCCaaaaaaaaaagaaacaatttaag                                                                                                                                                                                              >  2:886347/1‑226 (MQ=255)

                                             gttATTCAAACGTTTCAAGGGTGTAAAACGGTACGAAGTTATCGATATTTGTTCCCTGAAACATGGTGTGACGTTAGCGAAAGCAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCGGAGCTTGAAGCCATCGAAGGGGCGGGAGCGGTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGc                                                                                                                                                                                          <  2:439946/236‑1 (MQ=255)

                                              gttttCAAACGTTTTAAAGGTGTAAAACTGTACGAAATTATCGATATTGGTTCCCTTGAACATGCTGTTACGGTAGCGAAAACAGAAGGTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCCGTAAGCCATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCAGTCTATGGGc                                                                                                                                                                                          <  2:407901/234‑1 (MQ=255)

                                              ggtttCAAACTTTTAAAAGGGTTAAAACGTTACGAAATTATTGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTGTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGc                                                                                                                                                                                          <  2:556840/234‑1 (MQ=255)

                                              gTATTCAAACTTTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGt                                                                                                                                                                                                                                  <  2:142045/197‑1 (MQ=255)

                                              gTATTCAAACGTTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGt                                                                                                                                                                                                                                  >  1:142045/1‑197 (MQ=255)

                                              gTATTCAAACGTTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGGGT‑GGTATCTAACGGCCCAATTAACAAAATGCAGCATTCTATGGCCa                                                                                                                                                                                         >  1:708607/1‑238 (MQ=255)

                                              gTATTCAAACGTTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGACGGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACTGCCCAAATAACAAAATACAGCATTCTATGGCCaa                                                                                                                                                                                        >  1:339249/1‑239 (MQ=255)

                                               tgtttaaaCGTTTCAAAGGTGTAAAACTGTTCGAAATTATCGATATTGTTTCCCTTGAACATGGTTTTACGTTAGGGAAACCAGAAGCTGAACACGTTTACCGGGCAGAAGTCGCTCGGCTGTTCGATTCAGATCTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAg                                                                                                                                                                                       <  2:708607/234‑1 (MQ=255)

                                                aatCAAACTTTGCAAAGGTGAAAAACTGTAAGAAATTATCGATATTGTTACCCTTGAACATGGTGTTACGGTAGCGAAAACAGAAGCTGAACACGTTTACCGTTCAGAAGTCGCTCGGCTGTTCGAGTCAGAACTGGAAGCCATCGAAGGGGCTGTAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑CGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAg                                                                                                                                                                                       <  1:942155/236‑1 (MQ=255)

                                                 ttCAAACGTTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGAAGCATTCTATGGGCAAGCt                                                                                                                                                                                     >  1:387768/1‑239 (MQ=255)

                                                   cAAACGTTTCAAAGGTGTAAAACGGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGa                                                                                                                                                                                   <  1:168660/239‑1 (MQ=255)

                                                           tCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATTGGTGT‑GGTACCTAACGGCCCAAATAACAAAATGCAGCATTCCATGGGCAAGCTGAATATGtt                                                                                                                                                                            >  1:824155/1‑238 (MQ=255)

                                                                    tAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCACATAACAAAATGCAGCATTCTATGGGCAAGCTGACAATGTTGCACTACtt                                                                                                                                                                   >  1:372348/1‑238 (MQ=255)

                                                                    tAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAg                                                                                                                                                                                       >  1:1085853/1‑218 (MQ=255)

                                                                    tAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAg                                                                                                                                                                                       <  2:1085853/218‑1 (MQ=255)

                                                                    tAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAatactgtctcttttca                                                                                                                                                                   >  1:943687/1‑225 (MQ=255)

                                                                    tAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGGGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGaa                                                                                                                                                                                  >  1:646352/1‑223 (MQ=255)

                                                                    tAAAACTGTACGAAATTATCGAAATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGGTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATggg                                                                                                                                                                                           <  2:465967/214‑1 (MQ=255)

                                                                        aCTGTACGAAATTATCGATATTGTTTCCCTTGAACAGTGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCgg                                                                                                                                                              <  1:8978/239‑1 (MQ=255)

                                                                           gTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCCCTACTTCCCGGTTa                                                                                                                                                           >  1:716061/1‑239 (MQ=255)

                                                                            tACGAACTTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCGGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGCTCACAGCGCCCATGGGTGT‑GGGATCTTACGGCCCAAATAACAAAATTCAGCATTCTTAGGGAAAGCCTAAAATGTTGCACAACTTCCCGGATaa                                                                                                                                                          >  2:676745/1‑239 (MQ=255)

                                                                            tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATaa                                                                                                                                                          >  2:15872/1‑239 (MQ=255)

                                                                            tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAAAACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATACCAAAATGCAGCATTCTTTGGGCAAGCTGAATATGTTGCACTACTTCCCGGCTaa                                                                                                                                                          >  1:147975/1‑239 (MQ=255)

                                                                                      tCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACAACTTCCCGGATAAACTGTTCAGc                                                                                                                                                >  1:877385/1‑239 (MQ=255)

                                                                                       cGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg                                                                                                                                                                                                                                       <  1:1081320/152‑1 (MQ=255)

                                                                                        tattttGTTTTCCTTTGACAGGGGGTTGCGTTAGGGAAACCAGAAGCTGAACACGTTTCCCGTGAAGGAAGCGCTGGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGTGCTGGAGCGCTCCTGTCAGAGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCgg                                                                                                                                              <  2:388125/235‑1 (MQ=255)

                                                                                                 tCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGATGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGATCCTGTCAGCGATCACTGCGCCCATGCGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCCAAATATGTTGCAATACTAACCGGATACAATGTTCAGGGGCTACGatat                                                                                                                                     >  2:895435/1‑239 (MQ=255)

                                                                                                    cTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATCAACTGTTCAGCGGCTACGATATTCa                                                                                                                                  >  1:19539/1‑239 (MQ=255)

                                                                                                     ttgaaaagtgtttttcGTTAGCGAAAACAAAAGCCGAACACTTTTACCGGTAAAAAGTCCGCCGGCTGGTCGATTCAGAACTGGAAGCCAGCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAg                                                                                                                                 <  2:951420/224‑1 (MQ=255)

                                                                                                     aagtagcaggTTTTACGTTAGCGAAAACAGAAGCTGAACCCGTTTACCGTGCAGAAGTCCGTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGTCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCAGTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAg                                                                                                                                 <  2:198382/231‑1 (MQ=255)

                                                                                                        aaCATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATCACACAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCactcct                                                                                                                                                                    >  1:526925/1‑201 (MQ=255)

                                                                                                             ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCCAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTAACCGGCACGGATATTCAGCGCTGGaa                                                                                                                         >  1:487670/1‑239 (MQ=255)

                                                                                                             ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCgg                                                                                                                                              <  1:858497/218‑1 (MQ=255)

                                                                                                             ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGGATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTTTTCAGCGCCTACGATATTCAgcgcgtgaa                                                                                                                         >  1:61429/1‑234 (MQ=255)

                                                                                                                   aCGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATggg                                                                                                                                                                                           >  1:124899/1‑167 (MQ=255)

                                                                                                                       tAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGAGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGAAAAACTGTTCAACGGCCACAAAATTCAGCCCTGGAAACCAAACCCg                                                                                                               >  2:578824/1‑239 (MQ=255)

                                                                                                                        aGCGAGAACAGAAGTCGAACACGTTTACCGGGCAGGAGTCGCTCGGCTGTTGGAGCCAGAGCTGGAAGCCGTCGAAGGGGTTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAACTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCgg                                                                                                              <  1:495160/239‑1 (MQ=255)

                                                                                                                        aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATg                                                                                                                                                                                                         <  1:72618/148‑1 (MQ=255)

                                                                                                                        aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCgg                                                                                                              <  1:15872/239‑1 (MQ=255)

                                                                                                                        aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGGCAGCGATCACTGCGCCAATGTGGGT‑GGTATCTAACGGCCCAAATAACAAAATg                                                                                                                                                                                                         >  2:72618/1‑148 (MQ=255)

                                                                                                                         gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCaaa                                                                                                                                                                                                                   <  1:46127/137‑1 (MQ=255)

                                                                                                                         gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCgg                                                                                                                                                              >  2:639399/1‑190 (MQ=255)

                                                                                                                         gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTAGGATATTCAGCGCTGGAAGc                                                                                                                       >  1:198382/1‑229 (MQ=255)

                                                                                                                         gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCCGCATATGTTGAACACCTTCCCAGATAAACTGTTCCGCCGCTCGGAAATTCAGCGCTGGAACCCaaccacggg                                                                                                             >  2:594108/1‑231 (MQ=255)

                                                                                                                         gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGGGGGT‑GGGATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGCCAAGCGAAAAATGTTGCCCTACATCCCGGATAACCTGTTCAAGGGCACCCACATTCACCCCTCGACGCACGACCCGGc                                                                                                             >  2:760506/1‑239 (MQ=255)

                                                                                                                         gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCACCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGGATCTAACGGCCCAAATAACACAATGCAGCATTCCATGGGCAAGCTGAATATGTTGCACAACTTCCCGGAAAAACTGTTAAACGCCACCGAAATTAAACGCTGGAAACCAAACCCGGc                                                                                                             >  2:429598/1‑239 (MQ=255)

                                                                                                                         gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAATCGCTCGGATGTTCGATTCGGAACTGGAAGCAATCGAAGCGGCTGGAGCGCTCCTGTCAGCGATAACTGCGCCCATAGGGGA‑GGTATCTAAACGCCCAAAGAAAAAAATGCAGCATTCTATCGGCAAAGCAAAAAAGTGGCAATATTTCCCGGGAAAAATGTACATCCGCCAAGAAATTCAACGCGGGAAGCCaaagcgagg                                                                                                             >  2:1046071/1‑231 (MQ=255)

                                                                                                                              aaCAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCCATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGc                                                                                                                                                                                          >  2:371189/1‑157 (MQ=255)

                                                                                                                              aaCAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGc                                                                                                                                                                                          <  1:371189/157‑1 (MQ=255)

                                                                                                                                       tGAACCCTTTTTCCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCCCCGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCGGCGTTAATGTTCCa                                                                                                 <  2:266337/237‑1 (MQ=255)

                                                                                                                                               tttACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGGAAAACTGTTCCGCGGCTACGATATTAAGCGCTGGAAGCCAGACCCGGCGTTAATGTTCCCTGCGGCaaca                                                                                       >  1:1038608/1‑237 (MQ=255)

                                                                                                                                                   ccGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCGGCGTTACTGTTCCATGCGGCAAAAGCGa                                                                                   >  1:102079/1‑239 (MQ=255)

                                                                                                                                                    cGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCGGCGTTAATGTTCCATGCGGCAAAAGCGAt                                                                                  <  1:429598/239‑1 (MQ=255)

                                                                                                                                                      tGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAACTAACAAAATGCAGCATTCTATGGGCAAGCGGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGAAATTCCGCGCTGGAAGCCAGACCCGGCGCTACTGTTCCAAGCGGCAAAAgggcgga                                                                                >  2:271379/1‑233 (MQ=255)

                                                                                                                                                        cAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCGGCGTTAATGTTCCATGCGGCAAAAGCGATGaat                                                                              >  1:127399/1‑239 (MQ=255)

                                                                                                                                                             gTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATa                                                                                                                                                           >  1:248080/1‑157 (MQ=255)

                                                                                                                                                                gCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATACCAAAATGCAGCATTCTATGGGCAAGCGGAATATGTTTCACAACTTCCCGGATAAACTCGTCAACGGCCACGAAATTCAGGGCGGGAAGCCAGACCCGGGGTTAATTGTCCCTACGGCaaaaaggagaaaataaaagta                                                                      >  2:564082/1‑222 (MQ=255)

                                                                                                                                                                   cGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGACCGATCCGGGCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCCCTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCGGCGTTAATGTTCCATGCGGCAAAAGCGATGAATGTAAATGTAGa                                                                   <  1:609201/239‑1 (MQ=255)

                                                                                                                                                                   cGGCTGTTCGATGCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGGCAATGTGTGG‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCGGCGTTAATGTTCCATGCGGCAAAAGCGATGAATGTAAATGTAGa                                                                   <  1:314859/239‑1 (MQ=255)

                                                                                                                                                                     gCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCGCCGTTAATGTTCCATGCGGCACAACCGCTAGATGTGAATGAAGaaa                                                                 >  1:1101380/1‑239 (MQ=255)

                                                                                                                                                                      tttttCGATTCAAAACTGGAAGGCATGGAAGGGGCTGGGGCCCTCCTGTCAGCGATCACTGCGCCAATGTTGGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCGGCGTTAATGTTCCATGCGGCAAAAGCGATGAATGTAAATGTAGaaaa                                                                <  2:716061/236‑1 (MQ=255)

                                                                                                                                                                         ttCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCCACGATATTCAGCGCTGGAAGCCAGACCCGGCGGTAATGTTCCCTGCGGCAAAAACGGTAaaagtaaaaggaagaaacag                                                             >  2:803845/1‑227 (MQ=255)

                                                                                                                                                                                     cttgAAGCCATCGAAGGGGCTGGGGCGCTCCTGTAAGCGGTCACTAGGCCAATGTGGGT‑GTTTTCTAACTGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCTGCTACGATATTCAGCGCTGGAAGCCAGACCCGGCGTTAATGTTCCATGCGGCAAAAGCGATGAATGTAAATGTAGAAAACTGCATTCTGGTtga                                                 <  2:597459/236‑1 (MQ=255)

                                                                                                                                                                                        gAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAACCTGTTCAGCGGCTACGGTATTCACCGCTGGAAGCCCGACCCGGGGTTAATTTCCCATGTGGCAAAAGCGAGGAAGGTAAAATAAGAAAAcccaaatctggatgtgg                                               >  2:687200/1‑222 (MQ=255)

                                                                                                                                                                                         tataagggacaGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGGGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCGGCGTTAATGTTCCATGCGGCAAAAGCGATGAATGTAAATGTAGAAAACTGCATTCTGGTTGATGAc                                             <  1:767499/229‑1 (MQ=255)

                                                                                                                                                                                             cATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGc                                                                                                                                                                                          <  1:746449/94‑1 (MQ=255)

                                                                                                                                                                                              attgatggggCTGGGGCCCCCCTGGTCGGGATCACTGCTCAAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCGGCGTTAATGTTCCATGCGGCAAAAGCGATGAATGTAAATGTAGAAAACTGCATTCTGGTTGATGACTCAGt                                        <  2:678717/233‑1 (MQ=255)

                                                                                                                                                                                                cGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCGGCGTTAATGTTCCATCCGGCAAAACCCATGAAGGTAACTGGAGAACACTGCCTTCTggcgtgtgacccagtt                                       >  1:446495/1‑224 (MQ=255)

                                                                                                                                                                                                 gAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCGGCGTTAATGTTCCATGCGGCAAAAGCGATGAATGTAAATGTAGAAAACTGCATTCTGGTTGATGACTCAGt                                        <  2:127399/236‑1 (MQ=255)

                                                                                                                                                                                                 gAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCGGCGTTAATGTTCCATGCGGCAAAAGCGATGAATGTAAATGTAGAAAACTGCATTCTGGTTGATGACTCAGTTGc                                     <  1:803845/239‑1 (MQ=255)

                                                                                                                                                                                                      ggCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCGGCGTTAATGTTCCATGCGGCAAAAGCGATGAATGTAAATGTAGAAAACTGCATTCTGGTTGATGACTCAGTTGCCGGt                                 <  1:388713/238‑1 (MQ=255)

                                                                                                                                                                                                      ggCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCGGCGTTAATGTTACATGCGGCAAAAACGCTGCATGCAAATGTAGAAAACTGCCTTCTGGTTTTTGAACAAGTTGCCGGt                                 >  1:312509/1‑238 (MQ=255)

                                                                                                                                                                                                         tGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGGGTGT‑GGGATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCGGCGTTAATGTTCCATGCGGCAAAAGCGATGAATGTAAATGTAGAAAACTGCATTCTGGTTGATGACTCAGTTGCCGGTGca                              <  1:952859/238‑1 (MQ=255)

                                                                                                                                                                                                          ggAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCATCGGCTACGAAATTCAGCGCTGGAAGCCAGACCCGGCGTTAAAGTTCCATGCGGCAAAAGAGATGAATGGAAAGTCAAAAAAATGCATGCGGGTTGATGAAAAAGTTGGCgggtcaaa                            >  2:812975/1‑233 (MQ=255)

                                                                                                                                                                                                                cTCCTGTTAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCGGAATATGTTGAACTACTTCCCGGATAAAATGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCGGCGTTAATGTTCCATGCGCCAAAAGGATTGAATGTAAAGTTAGAAAACCGCATACTGGTTGATGACAAACTTGCCggctagcttttata                      >  2:767499/1‑227 (MQ=255)

                                                                                                                                                                                                                cTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAata                                                                                                                                                                                <  1:100234/85‑1 (MQ=255)

                                                                                                                                                                                                                cTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTcc                                                                                                                                                                 >  1:223671/1‑100 (MQ=255)

                                                                                                                                                                                                                cTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCGGCGTTAATGTTCCATGCGGCAAAAGCGATGaatgtaaatgta                                                                     <  1:574503/192‑1 (MQ=255)

                                                                                                                                                                                                                cTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCGGCGTTAAGGTTCCATGCGGCAAAAGCGATGACTGTAAATGTAGAAAACTACATTCTGGTTTATGAACCAGTTGCCGTGGCAAAAACTgg                      >  2:117746/1‑239 (MQ=255)

                                                                                                                                                                                                                cTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCGGAATATGTTGCACTACTTCCCGTATAAACTGTTCACCGTCTACGATATTCAGAGCTGGAAGCCAGACCCGGCGTTAATGTTCCATGCGGCTAAAGCGATGACTGGAAATGTATAAAACTGCATTCTGGTAGATGACTCAGTTCCCGGAGCaaaaacggg                      >  2:388713/1‑234 (MQ=255)

                                                                                                                                                                                                                cTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCAGTCTATGGGCAAGCTGAATATGTTGCACTACTTcc                                                                                                                                                                 <  2:223671/100‑1 (MQ=255)

                                                                                                                                                                                                                cTCCTGTCAGCGAGCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAata                                                                                                                                                                                >  2:100234/1‑85 (MQ=255)

                                                                                                                                                                                                                           gATCCCTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCAGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCGGCGTTAATGTTCCATGCGGCAAAAGCGATGAATGTAAATGTAGAAAACTGCATTCTGGTTGATGACTCAGTTGCCGGTGCACAATCTGGTATCGACGc             <  1:594108/237‑1 (MQ=255)

                                                                                                                                                                                                                           gATCACTGCCGCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCGGCGTTAATGTTCCATGCGGCAAAAGCGATGAATGTAAATGTAGAAAACTGCATTCTGGTTGATGACTCAGTTGCCGGTGCACAATCTGGTATCGACGc             <  1:886347/237‑1 (MQ=255)

                                                                                                                                                                                                                              agacaggcCAATGTTTTT‑GGTATTCAACGGCCCAAATAACAAAATTCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCGGCGTTAATGTTCCATGCGGCAAAAGCGATGAATGTAAATGTAGAAAACTGCATTCTGGTTGATGACTCAGTTGCCGGTGCACAATCTGGTATCGACGCAGGTa        <  2:1014154/233‑1 (MQ=255)

                                                                                                                                                                                                                                  gcgcCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCGGCGTTAATGTTCCATGCGGCAAAAGCGATg                                                                                 >  1:418781/1‑162 (MQ=255)

                                                                                                                                                                                                                                  gcgcCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCGGCGTTAATGTTCCATGCGGCAAAAGCGAAGAAAGTAAATGTCGAAAACTGCATTCTGGTTGATGGCCCAGTTTCCGGTGAACAATCTGGGATCGACGCGGGTATGGa    >  2:1074545/1‑239 (MQ=255)

                                                                                                                                                                                                                                  gcgcCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACCGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCGGCGTTAATGTTCCATGCGGCAAAAGCGATg                                                                                 <  2:418781/162‑1 (MQ=255)

                                                                                                                                                                                                                                   cgcCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCGGCGTTAATGTTCCATGCGGCAAAAGCGATGAATGTAAATGTAGAAAACTGCATTCTGGTTGATGAc                                             <  1:467310/197‑1 (MQ=255)

                                                                                                                                                                                                                                    gcCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCGGCGTTAATGTTCCATGCGGCAAAAGCGATGAATGGAAATGTAGAAAACTGCATTCTGGTTGATGACTCAATTGCCGGTGCACAATCGGGTATCGAACAAGGAATGGAAg  >  2:137071/1‑239 (MQ=255)

                                                                                                                                                                                                                                    cccAATGT‑TGTGGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCGATGGGCAAGATGAATATGTTGCACTACTGCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCGGCGTTAATGTTCCATGCGGCAAAAGCGATGAATGTAAATGTAGAAAACTGCATTCTGGTTGATGACTCAGTTGCCGGTGCACAATCTGGTATCGACGCAGGTATGGaa   <  2:858539/237‑1 (MQ=255)

TGTAACGATGTTTCAGGAATTTGGTATTACGCTCGATCCTGAAGAGGTATTCAAACGTTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGT‑GGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCGGCGTTAATGTTCCATGCGGCAAAAGCGATGAATGTAAATGTAGAAAACTGCATTCTGGTTGATGACTCAGTTGCCGGTGCACAATCTGGTATCGACGCAGGTATGGAAG  >  NC_000913/3896850‑3897316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`