Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,103,632	A→C	18.4%	V448G (GTG→GGG)	cpxA ←	sensory histidine kinase in two‑component regulatory system with CpxR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,103,632	0	A	C	18.4%	222.9 / 5.3	98	V448G (GTG→GGG)	cpxA	sensory histidine kinase in two‑component regulatory system with CpxR
Reads supporting (aligned to +/- strand):  ref base A (48/27);  new base C (2/21);  total (50/48)
Fisher's exact test for biased strand distribution p-value = 2.25e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCTGCCGGGTTATCGAAAAGCT‑GGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGG‑AAAATCTGTT‑CGCGATCTTCCGGGCTAACGCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCC  >  NC_000913/4103398‑4103861                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                             ccGCTGCCGGGTTATCGAAAAGCT‑GGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCa                                                                                                                                                                                                                                           >  1:400754/1‑238 (MQ=255)        cGGGTTATCGAAAAGCT‑GGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCCGCAAATGCGAAGTTTAACTCCCCTTAAAAAAGGGCAACCAAATACCAAGCCGTaa                                                                                                                                                                                                                                    >  2:500178/1‑238 (MQ=255)         gggTTTTCCAAAAGGTGGGCCGCGGGCGATCAAAAAGGCCCGACGAGGCCGAAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCGGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGGAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTaaa                                                                                                                                                                                                                                   <  2:679573/239‑1 (MQ=255)               tCGAAAGCCT‑GGACGCGGAAGATGAAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑a                                                                                                                                                                                                                           <  1:643740/239‑1 (MQ=255)                     aGCT‑GGACGGGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGc                                                                                                                                                                                                                     <  1:819177/239‑1 (MQ=255)                     aGCT‑GGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCCAATGCGAAGTTTAACTACGCTTATACAGAGGCAACCAAATCACCACACGAAAACCCCCC‑AGAGGGc                                                                                                                                                                                                                     >  2:819177/1‑239 (MQ=255)                     aGCT‑GGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATCCGAAGGTTAACTCCGCTTATACACCGGCAACCAAATCAACAACCGAAAACCCCCC‑AGCGGGc                                                                                                                                                                                                                     >  2:691092/1‑239 (MQ=255)                           gACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTTCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCCAATACCCAGCCGTAAACCGCCC‑ACCGGGCTgcc                                                                                                                                                                                                                 >  1:526425/1‑236 (MQ=255)                             cggggACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTCCCCGCCGGTTGGGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGtctt                                                                                                                                                                                                               <  1:500178/235‑1 (MQ=255)                                  aCGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATCAAACGGCAACCAAACAACAACCCACAAACCCCCC‑AACGGGcgagattctgcc                                                                                                                                                                                                          >  2:572024/1‑227 (MQ=255)                                         aaaaGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATAAAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑CCCGGGCTGTCTTCGGCCTTCACCCa                                                                                                                                                                                                  >  2:976345/1‑239 (MQ=255)                                           aaGCGCCCACTGGGGGCAAAGATCAAAGTTTTTCCCCGCCTGGTGGGGGGAAAATAACCCCCGGAGGGTAGGGCTGATCAGCCGTTGTCAGCGCAGCATCAGGGACCCTGCTCAAATGCCGGATGCGGCGTAACCGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCCCCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGc                                                                                                                                                                                                <  2:876944/239‑1 (MQ=255)                                             ccgccggtggggCGGCAATAGTGAAATTTTTCCCCCCCGGTTGTGGGGAAAATAGCCCCCGGAGGGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCGCAAATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCa                                                                                                                                                                                              <  2:1025393/228‑1 (MQ=38)                                              cgcCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACACAGcccc                                                                                                                                                                                             >  1:830131/1‑237 (MQ=255)                                               cccgatggggCGGAAAGCTCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCa                                                                                                                                                                                              <  1:619543/228‑1 (MQ=255)                                                ccGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑ACCGGGCTGTCTTCTGCCTTAAACCAGCCACGa                                                                                                                                                                                           >  1:710718/1‑239 (MQ=255)                                                 cgacgggggggCAAGATCGAAAATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGa                                                                                                                                                                                           <  1:389009/229‑1 (MQ=255)                                                             aaGACCGAAATTTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCCCCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATg                                                                                                                                                                               <  1:379700/238‑1 (MQ=255)                                                                              ccgccgGTTGGGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGcc                                                                                                                                                          <  1:976345/239‑1 (MQ=255)                                                                                            gAAAATAACACCCGGAGTGTAGGCCTGATAAGACGCTATCACCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCAGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTACACCTCCC‑AGCGGGATGTCTTCCGCCTTCACCCAGCCACGATCCTGCTGAATTGCGG‑C‑TTCCAA‑AAACCCCAA‑CCACaaaaccga                                                                                                                                           >  2:1102405/1‑234 (MQ=255)                                                                                                  aaCCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑G‑TTCCAC‑AAAACCCAC‑CCCCCAAccccaaccccc                                                                                                                                     >  2:163279/1‑230 (MQ=255)                                                                                                  aaCCCCCGGAGTGTAGCCCATGTAAGCCGCTCACGGCGTCGCATCGGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAAGGCGAAGTTTAACGCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGcc                                                                                                                                     <  1:701800/239‑1 (MQ=255)                                                                                                    cccccGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGCG‑TTAAAC‑AAAC‑ACAG‑CCCCAAAACCATACCCCCac                                                                                                                                   >  2:132066/1‑238 (MQ=39)                                                                                                    cccccGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑CATCGCCAG‑CCCCCAACCTggcccccccg                                                                                                                                   >  1:343366/1‑230 (MQ=255)                                                                                                         ggAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCCGCCACGATGCTGCTGAATGGCGG‑G‑TTCACC‑AATCCCCAC‑CCCCCAAccccaccaccccgatac                                                                                                                               >  2:525811/1‑223 (MQ=255)                                                                                                               tAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCCAACCtcgtccccccaaattacgatt                                                                                                                         >  1:815217/1‑218 (MQ=255)                                                                                                               tAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AAACGCCAG‑CCCCAAACCTGTACCCCCAGATTCACAATc                                                                                                                         >  1:428938/1‑238 (MQ=255)                                                                                                                     tGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCCGGTTCACGAACGCGTGCt                                                                                                                  >  1:687778/1‑239 (MQ=255)                                                                                                                       aTAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCAc                                                                                                                                                                                                                                             >  2:162489/1‑119 (MQ=255)                                                                                                                       aTAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCAc                                                                                                                                                                                                                                             <  1:162489/119‑1 (MQ=255)                                                                                                                       aTAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCAc                                                                                                                                                                                                                                             >  2:929456/1‑119 (MQ=255)                                                                                                                       aTAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCAc                                                                                                                                                                                                                                             <  1:929456/119‑1 (MQ=255)                                                                                                                       aTAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCCCGATGCCCCTGAATGGCGG‑TGTTCACC‑AA‑CGCCCC‑CCCCACACCCGTACccccgatgttcccaagacgtggcct                                                                                                                >  2:700522/1‑218 (MQ=255)                                                                                                                       aTAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCACC‑AATCCCCCG‑CCCCCAACCCGTACCGCCCGATTAACGTTCGCGTGCtc                                                                                                                 >  1:499458/1‑237 (MQ=255)                                                                                                                       aTAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCTCCAA‑CCCCCAAACTGTACCGCCAGATTCACGAACGCGGCCTTc                                                                                                                >  1:941122/1‑239 (MQ=255)                                                                                                                       aTAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCCAACCCTTACCCCCCGATTCACGATCCCGGGCTTc                                                                                                                >  1:1055686/1‑239 (MQ=255)                                                                                                                       aTAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTTGACCGCCCGATTTACCATCGCGTGCtt                                                                                                                 >  1:718055/1‑238 (MQ=255)                                                                                                                       aTAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑G‑TTCAAC‑AAACCCCAC‑CCCCCAAACCGTACCCCCaaaatacccacccccgggct                                                                                                                 >  2:413929/1‑219 (MQ=255)                                                                                                                       aTAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCGGTCTTCTGCCTTCACCCAGCCACCATCCTGCTGAATGGCGG‑G‑TTTCAC‑AATCGCCAC‑CCCCCAACCCCTACcccccccatccacctacgcggctt                                                                                                                 >  2:673834/1‑218 (MQ=255)                                                                                                                       aTAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCACCC‑AGCGGCCTGTCTTCTGCCTTCCCCCAGCCACGATGCTGCTGAATGGCGG‑G‑TTCACCAAACCGCCA‑‑CCCCCAACCCCTACCGCCCCATTCAccaaccccaccctc                                                                                                                >  2:348547/1‑226 (MQ=255)                                                                                                                         aaGACGCTTGCAGCGTCGCACCAGGCATCCTGCTAAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCCCCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCAt                                                                                                              <  1:794192/239‑1 (MQ=255)                                                                                                                            cccgtagccgCGTCGCATCGGGCATCCTGCTCAAGTGCCGGATGCGGCGTAAACCGCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCgg                                                                                                           <  1:96150/230‑1 (MQ=255)                                                                                                                            ccccaaccggCGTCCAATCAGGCATCCTGCTCAAAGGCCGGATGCGGCTTAAACGCCTTACCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCgg                                                                                                           <  1:213055/230‑1 (MQ=255)                                                                                                                             cgctgcccggtggccactaGGCATCCTGCTAAAAGGCCGGGTGCGGGCTAAACGCCTTAACCTGCCGGCAATTTCGAAGTTTACCCCCGCTTAACCGCCGGCACCCAACGCCCCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCCCCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCgg                                                                                                           <  2:84126/220‑1 (MQ=255)                                                                                                                              gCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCCAA‑CATTGCCAA‑CCCCAAACCTGTACCCCACAATTTACGAGCCCGCGCTTCATCggc                                                                                                          >  1:809421/1‑237 (MQ=255)                                                                                                                                 aTCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCCAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑G‑TTAAAA‑CATAGCCAGACCCCAAACC‑GTACCCCCCGATACCCGATCCCGTGCTTAATCGGTACgc                                                                                                      >  1:42440/1‑238 (MQ=255)                                                                                                                                 aTCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCCGTACCGCCAGATTCACGactgtctcttttacacctctc                                                                                                       >  1:254880/1‑218 (MQ=255)                                                                                                                                 aTCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTCCTGAATGGCGG‑G‑TTCAAC‑AATCCCCAG‑CCCCCAACCCTTACCGCCCGATTCCCAAACGCGTGCTTCATCgggccaa                                                                                                      >  2:684872/1‑234 (MQ=255)                                                                                                                                  tCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑CATCGCCAG‑CCCCAAACCCTGACCCCCCGATTCACGATCCCCGGCTTCCTccggaggaa                                                                                                     >  2:772826/1‑231 (MQ=255)                                                                                                                                      cGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGGGCTTCCTCGGTACAATGGaa                                                                                                 >  1:925831/1‑239 (MQ=255)                                                                                                                                         cgcctcgggcccctgtcTCAAAGCCCGGGGGCGGGGTAAACCCCTTACCCTGCCTGGAAATGCAAAGTTTAACCCCCCTTATCCAGCGGCAACCAAACCCCCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCCCGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAacg                                                                                               <  2:499458/222‑1 (MQ=255)                                                                                                                                         cccATCCGGCATCGTGTCCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATTCAGCGGCAACCAAATCCCCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAacg                                                                                               <  1:268945/236‑1 (MQ=255)                                                                                                                                         cGCATCAGGCATCCTGCCAAAATGCCGGATGCGGCGGAAACGCCTTAGCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAacg                                                                                               <  1:623270/238‑1 (MQ=255)                                                                                                                                                   aTCCTGCTCAAAGGCCGGATGCGGCGTAACCGCCTTATCCTGCCTGCAAATGCGAAGTTTAACCCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGG‑AAAAt                                                                                    <  1:572024/238‑1 (MQ=255)                                                                                                                                                       tGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑G‑TTCCAC‑AATCGCCAA‑CCCCCAAACTGCACCGCCAGATTCCCGATCGCCTGCTTCACCCGGACAAAAAAACGAAAGG‑aaaaacata                                                                                >  2:806745/1‑233 (MQ=255)                                                                                                                                                         cTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCCAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGGTAGAACGGACGG‑GAAATCTGttt                                                                              >  1:758186/1‑237 (MQ=255)                                                                                                                                                           taaagtccggtgggggcgAAAACCCCTTTCCCTGCCTGCAAAGGGGAAGTTTAACCCCGCTTATAACGCGGCAACCAAATCCCCAGCCGTAAACCGCCA‑AGCGGGCCTTCTTCTGCCTTCCCCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAACCCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGG‑AAAATCTGTT‑cgcg                                                                          <  2:1007726/225‑1 (MQ=255)                                                                                                                                                           cAAAGGCCGGTGGCGGCTTAACCGCCTTTCCCGGCCTGCAAATGCGAAGTTTACCTCCGCTTATCCGGCGCAAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGG‑AAGATCTGTT‑cgcg                                                                          <  1:580412/239‑1 (MQ=255)                                                                                                                                                                       ggggcgTAAACGCCTGCTCCTGGCTGAAAATGCGAAGTTAAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGG‑AAAATCTGTT‑CGCGATCTTCCGGGCt                                                              <  1:201829/237‑1 (MQ=255)                                                                                                                                                                       gcggcgTAAACGCCTTACCCTGCCTGCAAATGCGAAGTTTAACCCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGG‑AAAATCTGTT‑CGCGATCTTCCGGGCt                                                              <  1:413929/239‑1 (MQ=255)                                                                                                                                                                        cggcgTAAACGCCTTAGCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGG‑AAAATCTGTT‑CGCGATCTTCCGGGCt                                                              <  1:691092/238‑1 (MQ=255)                                                                                                                                                                        cggcgTAAACCCCTTACCCTCCCTGCAAATGCGAATTTTACCTCCGCTTATACAGCGGCAACCAAATCCCCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCCCCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGG‑AAAATCTGTT‑CGCGATCTTCCGGGCt                                                              <  1:700522/238‑1 (MQ=255)                                                                                                                                                                                    cTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCCCCCGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGG‑AAAATCTGTT‑CGCGATCTTCCGGGCTAACGCCAGGACCa                                                 <  1:776899/239‑1 (MQ=255)                                                                                                                                                                                    cTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGG‑AAAATCTGTT‑CGCGATCTTCCGGGCt                                                              <  1:932569/226‑1 (MQ=255)                                                                                                                                                                                     ttATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCCAACCTGTACCGCCAGATTCACGATCGCGGGCTTCATCGGTACGGTAGAACGGACGG‑GAAAACTTTT‑CGCGATCTTCCGGGCTAAAGCCCGGAcc                                                  >  1:253300/1‑237 (MQ=255)                                                                                                                                                                                     ttATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCCAACCCGTACCGCCCGATTCACGATCGCGTGCTTCATCGGGACGATAAAACGGACGG‑AAAATCTGTT‑CCCGATCTTCCCGGCTAACCCCCGGaacc                                                 >  1:947964/1‑235 (MQ=255)                                                                                                                                                                                     ttATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCCGATTCACGATCGCGTGCTTCATCGGTACGAATGAACGGACGG‑AAAATATGTT‑CGCGACCTTCCGGGCTAACCCCAGGACCa                                                 >  1:169256/1‑238 (MQ=255)                                                                                                                                                                                            gcctgcAAATGCTAAGTTTAACTCCGCTTAGACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGGCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGG‑AAAATCTGTT‑CGCGATCTTCCGGGCTAACGCCAGGACCATCgtcgtc                                         <  1:33528/239‑1 (MQ=255)                                                                                                                                                                                            gcctgcAAATGCGAATTTTAACCCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGG‑AAAATCTGTT‑CGCGATCTTCCGGGCTAACGCCAGGACCATCgtcgtc                                         <  1:326519/239‑1 (MQ=255)                                                                                                                                                                                            gcctgcAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCCCCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCCCCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGG‑AAAATCTGTT‑CGCGATCTTCCGGGCTAACGCCAGGACCATCgtcgtc                                         <  1:295987/239‑1 (MQ=255)                                                                                                                                                                                            gcctgcAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGG‑AAAATCTGTT‑CGCGATCTTCCGGGCTAACGCCAGGACCATCgtcgtc                                         <  1:772826/239‑1 (MQ=255)                                                                                                                                                                                            gcctgcAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGG‑AAAATCTGTT‑CGCGATCTTCCGGGCTAACGCCAGGACCATCgtcgtc                                         <  1:246663/239‑1 (MQ=255)                                                                                                                                                                                             cctgcAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCCGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGG‑GAAATCTGTT‑CCCGATCTTCCGGGCTAACGCCAGGACCATCgtcgtc                                         >  1:222518/1‑238 (MQ=255)                                                                                                                                                                                             cctgcAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGGACGATAGAACGGACGG‑AAAAACTGTT‑CGCGATCTTCCCGGCTAACGCCAGGACCATCgtcgtc                                         >  1:85992/1‑238 (MQ=255)                                                                                                                                                                                              ctgcAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTTCAC‑AATCTCCAA‑CCCCCAACCTTTACCGCCCGATTCACCGTCACGTGCTTCATCGGCACGCTACAACAGCAGG‑GAAATCTGTT‑CGCGGCCTTCCGGGGCAACCCCACGACCATCGCCGccc                                        >  2:404736/1‑238 (MQ=255)                                                                                                                                                                                              ctgcAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCCAACCTGTACCCCCAGATTCACGATCCCGGGCTTCATCGGTa                                                                                                         >  1:972416/1‑175 (MQ=255)                                                                                                                                                                                                  aaaTGGAAATTTTAACCCCGTTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGG‑AAAATCTGTT‑CGCGATCTTCCGGGCTAACGCCAGGACCATCGTCGTCCACCGt                                   <  1:605863/239‑1 (MQ=255)                                                                                                                                                                                                  aaaTGCGAAGTTTAACCCCGCTTCAACGGCGGCAACCAAATCCCCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCCCCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGG‑AAAATCTGTT‑CGCGATCTTCCGGGCTAACGCCAGGACCATCGTCGTCCACCGt                                   <  1:4546/239‑1 (MQ=255)                                                                                                                                                                                                    aTGCGAATTTTCATCCCTCTTAAACGGCGGAACCAAAAACCCCGCCCGAAACCCGCCC‑AGCGGGCTGGCTGCTGCCTTCACCCAGCCACGATGCTGCTGAATGCCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAGCCTGTTCCGCCAGATTCACGATCGCGTGCTTCAGCGGTACGATAGAACGGACGG‑AAAATCTGTT‑CGCGATCTTCCGGGCTAACGCCAGGACCATCGTCGTCCACCGTaa                                 <  2:710718/239‑1 (MQ=255)                                                                                                                                                                                                    aTGCGAAGTTTAACCCCGCTTATACAGCGGCCACCAAATCCCCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGG‑AAAATCTGTT‑CGCGATCTTCCGGGCTAACGCCAGGACCATCGTCGTCCACCGTaa                                 <  1:190617/239‑1 (MQ=255)                                                                                                                                                                                                            tttAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGG‑AAAATCTGTT‑CGCGATCTTCCGGGCTAACGCCAGGACCATCGTCGTCCACCGTAATGGTGATa                         <  1:397998/239‑1 (MQ=255)                                                                                                                                                                                                             ttactccggtttttcaccgggCAACCAACCCCACTGCCTTAACCCGCCC‑GGCGGGCTGTCTTCTGCCTTCCCCCAGCCCCGATGGTGCTGATTGGCGG‑T‑TTCACC‑ATTCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGTTCGCGTGCTTCATCGGTACGATAGACCGGACGG‑AAAATCTGTT‑CGCGATCTTCCGGGCTAACGCCAGGACCATCGTCGTCCACCGTAATGGTGATAc                        <  2:809421/220‑1 (MQ=255)                                                                                                                                                                                                             ttAACTCCGCTTATACGGCGGCAACCAAATCCCCAGCCGTAAACCGCCC‑AGGGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGG‑AAAATCTGTT‑CGCGATCTTCCGGGCTAACGCCAGGACCATCGTCGTCCACCGTAATGGTGATa                         <  1:525811/238‑1 (MQ=255)                                                                                                                                                                                                             taaaagagacagaaccggCGGAAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTAAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGG‑AAAATCTGTT‑CGCGATCTTCCGGGCTAACGCCAGGACCATCGTCGTCCACCGTAATGGTGATa                         <  1:826149/221‑1 (MQ=255)                                                                                                                                                                                                              gaaCTCCGTTTATAAAGCGGCAACCAAAGCCCCAGCCGTAAACCGCCC‑AGCGGGCTGTCTACTGCCTTCACCCAGCCACGATCCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGG‑AAAATCTGTT‑CGCGATCTTCCGGGCTAACGCCAGGACCATCGTCGTCCACCGTAATGGTGATa                         <  1:39210/236‑1 (MQ=255)                                                                                                                                                                                                                aCTCCGCTTATACAGCGGCAACAAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCCCGTGCTTCACCGGTCCGCAAGAAAGGAAGGAAAAACCGGTTGCGCCAT‑TTCCGGGCT‑ACACCCGGACCCTCGCCGCCCAccccaaaggggaaaacc                      >  2:668155/1‑223 (MQ=255)                                                                                                                                                                                                                    ccgttttccggcgcCAACCAACCCCCCCGCCGTAAACCCCCC‑AGCGGGCTTTCCTCCGCCTTCCCCCAGCCCCGATGCTGCTGAATGGCGT‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGG‑AAAATCTGTT‑CGCGATCTTCCGGGCTAACGCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCttt                 <  2:343366/229‑1 (MQ=255)                                                                                                                                                                                                                    cGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAAACTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGG‑AAAATCTGTT‑CGCGATCTTCCGGGCTAACGCCAGGACCATCCTCGCCCACCGCAATGGGGGTACGGTAttt                 >  1:861378/1‑239 (MQ=255)                                                                                                                                                                                                                      cTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑CATCCCCAG‑CCCCCAACCTGTACCCCCCAATTCCCGATCCCGTGCTTCCCCGGGACCATCGAACAGACGG‑CAAAACTGTT‑CCCGATTTTTCCGGCTAACCCCCGGACCAACCGCCCCCACCCGAAATGTGATACCGTCCTTAt               >  2:472357/1‑239 (MQ=255)                                                                                                                                                                                                                      cTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGG‑AAAATCTGTT‑CCCGATCTTCCGGGCTAACGCCCGGa                                                    >  1:1033947/1‑202 (MQ=255)                                                                                                                                                                                                                      attATACAGCGGCAACCAACTCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑A‑TTTAAC‑AACTCCCCG‑ACCCCAACCTGGACCGCCAGAGTTCCGATCGAGTGCTTCATCCGTACGAAATAACGGGAGG‑GCAATCTTTT‑CTCGCTCTCCCGGGCTCCAGCCACAACCATGGACGTCAAACGTATAGGGGATAccccgctttt               >  2:657477/2‑231 (MQ=255)                                                                                                                                                                                                                              gCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGG‑AAAATCTGTT‑CGCGATCTTCCGGGCTAAAGCCCGGACCCTCGTCGCCCCCCGTAAAGGGGCTACCGCCTTTTTCTACCCCa       >  1:1010456/1‑239 (MQ=255)                                                                                                                                                                                                                              acgcccaCCAAATCCCCGCCCGGAACCCGCCCGTGGGGGCT‑TCTTCCGCCTTCACCCAGCCCCGATGCTGCGGAAGGGCAG‑T‑TTCAAC‑AAGCCCCCG‑CTCCAAACCTGTACCGCCCGATTCACGATCGCGTGCTTCATCGGTCCGATAGAACGGACGG‑AAAATCTGTT‑CGCGATCTTCCGGGCTAACGCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCa       <  2:1028562/233‑1 (MQ=255)                                                                                                                                                                                                                               cGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTTACGATCGCGTGCTTCATCGGTACGATAGAAAGGACGG‑AAAATCTGTT‑CGCGATCTTCCCGGCTAACGCCCGGACCACCGTCGTCCACCGCAATggctggcccttttaaacatctccga       >  1:449344/1‑215 (MQ=255)                                                                                                                                                                                                                               cGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCCCGTGCTTCATCGGTACGATAGAACGGACGG‑AAAATCTGTT‑CGCGATCTTCCGGGCTAACGCCAGGACCATCGTCGTCCCCCGTAATGGTGATACCGTCTTTTTTTACCGCaa      >  1:867853/1‑239 (MQ=255)                                                                                                                                                                                                                                ggCAACCAAATCCCCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCCGCCTTCACCCAGCCGCGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGG‑AAAATCTGTT‑CGCGATCTTCCGGGCTAACGCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCa       <  1:73263/237‑1 (MQ=255)                                                                                                                                                                                                                                   aaCCAAATCCCCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGG‑AAAATCTGTT‑CGCGATCTTCCGGGCTAACGCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGcc  <  1:163279/239‑1 (MQ=255)                                                                                                                                                                                                                                   aaCCAAATCCCCAGCCGGAAACCCGCC‑AGGGGGCTGTCTTCTGCCTTCCCCCAGCCGCGATGCTGCCGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGG‑AAAATCTGTT‑CGCGATCTTCCGGGCTAACGCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGc   <  1:635347/238‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                             CCGCTGCCGGGTTATCGAAAAGCT‑GGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCC‑AGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGG‑T‑TTCAAC‑AATCGCCAG‑CCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGG‑AAAATCTGTT‑CGCGATCTTCCGGGCTAACGCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCC  >  NC_000913/4103398‑4103861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑