Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,145,087	G→C	13.7%	R365P (CGC→CCC)	pflD →	putative glycine radical domain‑containing pyruvate formate‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,145,087	0	G	C	13.7%	165.8 / 3.9	80	R365P (CGC→CCC)	pflD	putative glycine radical domain‑containing pyruvate formate‑lyase
Reads supporting (aligned to +/- strand):  ref base G (52/16);  new base C (2/10);  total (54/26)
Fisher's exact test for biased strand distribution p-value = 1.33e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.18e-01

GGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑AGTGGTGGTGCCAGCGTTCCTCA‑ACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGA  >  NC_000913/4144851‑4145324                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                   gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTC‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTgcgc                                                                                                                                                                                                                                                   >  1:694031/1‑238 (MQ=255) gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTgcgc                                                                                                                                                                                                                                                   >  1:845335/1‑238 (MQ=255) gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTgcgc                                                                                                                                                                                                                                                   >  1:31972/1‑238 (MQ=255) gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCa                                                                                                                                                                                                                                                  >  1:90338/1‑239 (MQ=255) gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGGG‑GAA‑TTA‑CCGCA‑A‑CCGAACCCCGGCGTgcgc                                                                                                                                                                                                                                                   >  1:280732/1‑238 (MQ=255)  ggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGCGAACGTGCTTTCGGTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAAACTCGGCGGgcgcct                                                                                                                                                                                                                                                 >  1:428722/1‑237 (MQ=255)  ggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGAAGCACGGTGAACGGCCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑CCTTG‑CAA‑TTA‑‑CGCA‑ACCCCAACCTCGGCGTGCGCAc                                                                                                                                                                                                                                                 >  1:989899/1‑239 (MQ=255)    cGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACt                                                                                                                                                                                                                                                <  2:845335/238‑1 (MQ=255)       aGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGGAGCGCCGGGAACGGGCTTTCGGTCCCTT‑GCTT‑TGACCCCCA‑TCAAA‑GCGGG‑CAA‑TTA‑CCGCA‑A‑CCCAACCTCCGGCAGCGCACCAAc                                                                                                                                                                                                                                             >  2:123489/1‑238 (MQ=255)           tgggcgTTCCTTAAAGACCTGCTCCATTTTTTATGGGGGAAATGCAACGACACCGTGCTGTTGCGCTCCCCCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGGCC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑tt                                                                                                                                                                                                                                       <  2:694031/237‑1 (MQ=255)           gcggcgTTCCTGAAAGCTCTTCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCTCCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑tt                                                                                                                                                                                                                                       <  1:317181/239‑1 (MQ=255)                         agttcttcTCGAATCTTTGGGGGTAAAATGCACATGCCTCGGCCTTTTGCGCTCCACCGGTATGCCGCGTTTTTTCGCCGGTTCCCCCACC‑GGTTGGACCGCACTGCTCCGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACACGCCGt                                                                                                                                                                                                                        <  2:470567/232‑1 (MQ=255)                                cTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCCCTAACGCC‑TTGATCG‑ACACCCCGTTCCtgat                                                                                                                                                                                                                 >  1:552447/1‑239 (MQ=255)                                cTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACACGCCGTTCCtgct                                                                                                                                                                                                                 >  1:162356/1‑237 (MQ=255)                                            tgcgtaaatctcccccttctCGTGTTGTTGCTCTCCGCCATTGCCGGGGGTTATTCCGCGGGGTCCCCGCCT‑GGCTTGCCCGAGCTGTTGGGGGTGTTACCCGAGA‑AG‑GGCCGAGCCGCGGTTAACGTGTTTTC‑TTCCTTT‑TCCTGTGCCGCC‑A‑TCAAATGCTTG‑CAA‑TTC‑CCCCA‑C‑CTGAACCGCGCCGGGCGCACTACCGCC‑TGTTCCG‑ACACGCCGTTCCTGATGAAAACCGCCGa                                                                                                                                                                                                     <  2:549903/220‑1 (MQ=255)                                                  aaaTGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCAt                                                                                                                                                                                               >  1:525757/1‑239 (MQ=255)                                                  aaaTGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGGGCGCACTAACGCC‑TTGATCG‑AAACGCCGTTCCCGATGAAAACCGCCCAAAACAt                                                                                                                                                                                               >  1:468022/1‑239 (MQ=255)                                                  aaaTGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGGG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCCCCGAAACCAt                                                                                                                                                                                               >  1:297556/1‑239 (MQ=255)                                                         aCGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACCCGCCGTTCCTGTTGAAAACCGCCGAAACCATTCGCTTc                                                                                                                                                                                        >  1:679321/1‑239 (MQ=255)                                                          cGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGGGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCCA‑TCAAA‑GCGTG‑CAA‑TTA‑CCCCA‑A‑CCAGACCCCGCACGGCGCCCTAACCCC‑CTGACCGAACAC‑CCGCTCCGCATGCAAACCGCCCAAACCACTCGcg                                                                                                                                                                                          >  2:709527/1‑235 (MQ=255)                                                             cATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTC‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGGGCCGCCTACCGCC‑CTGATCG‑ACAACCCGTTCCTGCTGaaaaacccccaacaccacgccctctga                                                                                                                                                                                     >  2:184083/1‑215 (MQ=255)                                                                cGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCg                                                                                                                                                                                >  1:1085425/1‑239 (MQ=255)                                                                cGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACAAGCCGTTCCTGAGGAAAACCGCCGAAAACATTCGCTTCG‑GTACCg                                                                                                                                                                                >  1:789665/1‑239 (MQ=255)                                                                 gTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCCCCCTAACCCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCCCCGAAACCATTCGCTCCG‑GTACCgg                                                                                                                                                                               >  1:461000/1‑239 (MQ=255)                                                                  tGCTGTTGCGCGCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGCGA‑AC‑GGACGTAGCGCGGTGAAAGTGCTTTAGTTCCATT‑GCCT‑TGACGCCCA‑TCAAA‑CCGGG‑CAA‑TTA‑CCGCT‑A‑CCACACCTCGGCGTGCGCGCCAACCCC‑TTGAACA‑GCCCGCCGTTACCTAGGAGAGCGCCCGAAACAATTCGCTTCg‑ggcccgcg                                                                                                                                                                              >  2:819597/1‑232 (MQ=255)                                                                      gTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGCAACCATTCGCTTCG‑GTACCGGAAtt                                                                                                                                                                           >  1:209325/1‑238 (MQ=255)                                                                       ttGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACt                                                                                                                                                                                                                                                <  1:180958/171‑1 (MQ=255)                                                                       ttGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGGGCGCACTAAAGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTTGCTTCG‑GTACCGGGATTc                                                                                                                                                                          >  1:945127/1‑238 (MQ=255)                                                                       ttGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGGAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACt                                                                                                                                                                                                                                                >  2:180958/1‑171 (MQ=255)                                                                       ttGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACC‑GCCTATACCGCACTGCTCGGCTGGGTAACCGTGA‑AC‑TGACGTAGCGCGCTTTACGTGCTTTGGTTCCTTT‑GCCT‑CGATGCCTATTCAAA‑G‑GGG‑CCA‑TTA‑CCGCC‑A‑CCCGAGCTCGCACGGCGCCCTAACGCC‑TTCATCG‑GACGGCGGTTCCTGATGAAAACCGCCGAAAACATTCACATCG‑GAACCCGTAATcc                                                                                                                                                                         >  2:9472/1‑239 (MQ=255)                                                                            cTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CCA‑TTA‑CCGCC‑A‑CCGAACCTCGGCGTGCGCACTAAACCC‑TTGTTCG‑CACCGCCGTTCCCGATGAAAACCCCCCAAACCATCCCCTTCC‑GACCCGGCATTCCgacaa                                                                                                                                                                    >  2:436345/1‑235 (MQ=255)                                                                            cTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCACGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACCTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGGG‑CAA‑TTA‑CCGCA‑A‑CCAACCCTCCGCGGCCGCCCTAACCAC‑TTGACCG‑ACACGCCCTTCCAGTTACAAAACGCGCAAACCATCTGCTTCG‑GAACCggatttcgccaaa                                                                                                                                                                    >  2:100701/1‑228 (MQ=255)                                                                                 ccaGTAGCGCGCGTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCCGTATTCCCCACACCttt                                                                                                                                                               >  1:383763/1‑239 (MQ=255)                                                                                   tgtggcgcgcTTTGTTTC‑CGGGTTTCCCCCGCGGGCTATCCCGCCGTGC‑CGGGGGGTTAACCCAGA‑ACGGGACGTGGCGGGGGAAACTGTCTTCCTTTCTTTT‑GCCG‑TGCCCCCTT‑ACAAA‑GCTGG‑AAT‑TTA‑CCCCA‑C‑CCGACCCGGGGCGTCCCCACTAACGCC‑TTGATCG‑ACCCGCCTTTCCTGATAAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTaa                                                                                                                                                             <  2:297556/235‑1 (MQ=255)                                                                                       tcgggtttttttttcgtggTTTCCCGTCC‑GGCTCTGTCCCGCTTCGCTGGGGGTTACCCGAGAGAC‑GG‑CGCGGCGCGGTTCACGTGCTTTCGTTCCTTTGGCCG‑TGCCG‑CTA‑TAAAA‑GGCGG‑CAA‑TTT‑CCCCACA‑CCGCACCGGGGGGTGCCCTATAACCCG‑TTGATCG‑ACCCGCCGTCCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGa                                                                                                                                                          <  2:209325/222‑1 (MQ=255)                                                                                       gcgcgcGTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAAAGCC‑TTGGTCC‑ACACGCCGTTCCTTATGAAAACCCCCGAAACCATTCACTCCGTGACCCGGTTTTCCGAAAACTTTAAACGa                                                                                                                                                          >  2:259315/1‑239 (MQ=255)                                                                                         gcgcgTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGGGCACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGCATTCCCAAAATCTTTAACGATGa                                                                                                                                                       >  1:505184/1‑239 (MQ=255)                                                                                         gcgcgTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACCCGCCGTTCCTGCTGAAAACCGCCGAAACCATTCGCTCCG‑GTAACGGGATTCCCCAAACCTTTAACGATGa                                                                                                                                                       >  1:439187/1‑239 (MQ=255)                                                                                         gcgcgTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAAACGAGG‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACAAGCCGTTCCTCATCAAAACAGCCGGAACCATTCGCTTCG‑GGACCCGGCTTTCCCAAAACTTTTACGAAGa                                                                                                                                                       >  2:921647/1‑239 (MQ=255)                                                                                          cgcgTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTa                                                                                                                                                                                                                                               <  1:568839/153‑1 (MQ=255)                                                                                          cgcgTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTa                                                                                                                                                                                                                                               >  2:568839/1‑153 (MQ=255)                                                                                          cgcgTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAAACGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCCAACCTCCGCCTGCGCACTACAGCC‑TTGATTG‑CAACCCCATTCCCGTATAAAACCGCCGACAACCTACCTCTCG‑GACCCGGGAATTCGCAAAACTTTAACgcaaaa                                                                                                                                                      >  2:153241/1‑234 (MQ=255)                                                                                            cgTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGGATTCCCCAAATCTTTAACGCTGa‑aac                                                                                                                                                   >  1:448720/1‑237 (MQ=255)                                                                                                                               cACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GCACCGGTATTCCGCAAATCTTTAACGATGA‑AGGGGTTGTGCCAGCGTTCCTCA‑ACCGTGGGGTTTc                                                                                                                 >  1:57042/1‑238 (MQ=255)                                                                                                                                aCTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑AGAGGGGGTGCCAGCGTTCCCCA‑ACCGTGGCGgttgcc                                                                                                               >  1:733851/1‑236 (MQ=255)                                                                                                                                aCTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCCAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑AGTGGTGGTGCCAGCGTTCCTCA‑ACCGTGGCGTTTc                                                                                                                 >  1:195632/1‑237 (MQ=255)                                                                                                                                 cTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGAATTCCGCAAATCTTTAACGATGA‑AGGGGGGGGGCCAGCGTTCCTCA‑ACCTGGGGGTTTc                                                                                                                 >  1:419835/1‑236 (MQ=255)                                                                                                                                 cTGCTCGGCGGGGTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGGG‑CAA‑TTA‑CCGCC‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCA‑ACAAGCCGTTCCTGATGACAACCGACGAAACCACTCGCTTCC‑GTACCGGGATTCCCCAAAACTTTAAAGATGAGCGTGG‑GGTGCCCCGGATCCGCA‑ACCGTgggtgtttgcg                                                                                                              >  2:10923/1‑230 (MQ=255)                                                                                                                                    cTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCCCTAACGCC‑TTGATCG‑CCACGCCGTTCCTGCTGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGAATTCCGCAAAACTTTAACGAGGA‑AAGGGGGGGGCCACCGTGCCTCACACCG‑GGGGTTTCGCTGGa                                                                                                           >  2:1094833/1‑239 (MQ=255)                                                                                                                                    cTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑AGTGGGGGTGCCAGCGTTCCTCA‑ACCGTGGCGTTTTCCTGGa                                                                                                           >  1:878290/1‑239 (MQ=255)                                                                                                                                    cTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑AATGGTGGGGCCAGCGTTCCCCA‑ACCGGGGGGtttgccggga                                                                                                           >  2:452336/1‑232 (MQ=255)                                                                                                                                    cTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACACCCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGAATTCCGCAAATCTTTAACGATGC‑AGTGGGGGGTCCAGCGTTCCTCA‑ACCGGGGGGTTTCCCTGGa                                                                                                           >  2:82580/1‑239 (MQ=255)                                                                                                                                            gTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑AGGGGGGGTGCCAGCGTTCCTCA‑ACCGTGGCGTTTCCCTGGAAGAGgcgc                                                                                                   >  1:444110/1‑239 (MQ=255)                                                                                                                                                cccGGGA‑AG‑GGACTTAGCGGGGTGCAGGTTGTTGCGTCCCTTT‑GCTG‑TGCCCCCTA‑CAAAA‑GC‑TG‑TAACTGC‑CCGCA‑C‑CCAAACCCGGGCGGGCGCACTAACTCC‑TTGATCG‑ACACGCCGTTCCTGATAAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑AGTGGTGGTGCCAGCGTTCCTCA‑ACCGTGGCGTTTCGCTGGAAGATGCGCGCGa                                                                                               <  2:783674/238‑1 (MQ=255)                                                                                                                                                     aa‑aC‑GGACGTAGCGGGGTAAACGTGCTTTCGTTCCTTT‑GCCT‑TGACCCCTA‑ACAAA‑GCGTG‑AAA‑TTC‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCT‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑AGTGGTGGTGCCAGCGTTCCTCA‑ACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTAtt                                                                                          <  1:184083/238‑1 (MQ=255)                                                                                                                                                      a‑aC‑GGAGGTGGCGGGGTGAAGTTTTTTTCTTTCCTTT‑GCCT‑TGCCCCCTA‑ACAAA‑GGGTC‑AAA‑TCC‑CCCCA‑C‑CCGAACCGGGGGGTGCCCA‑TTACGCCTTTGACCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑AGTGGTGGTGCCAGCGTTCCTCA‑ACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTAtt                                                                                          <  2:444110/238‑1 (MQ=255)                                                                                                                                                      a‑aC‑GGACGTAGCGGGGTGACCGTGCTTTCGTCCCTTT‑GCCT‑GGACGCCTA‑TCAAG‑GCGTG‑CAA‑TTC‑CCGAA‑A‑CCGACCCTGGGCGTGCCCACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑AGTGGTGGTGCCAGCGTTCCTCA‑ACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTAtt                                                                                          <  1:709527/238‑1 (MQ=255)                                                                                                                                                       acc‑GGACGTAGTGCGGTGACCGTTCTTTCTTTCTTTT‑GCTT‑GCACGCCTA‑CAAAA‑GCGTG‑CAA‑TCA‑CGGCA‑C‑CCTACCCGCGGGGTCCGCACAACCGCC‑TTGACCG‑ACACGCCTTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑AGTGGTGGTGCCAGCGTTCCTCA‑ACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTAtt                                                                                          <  2:525757/236‑1 (MQ=255)                                                                                                                                                                 gcggcgtggAACTTTCTTTCTTTCCTTG‑TCTT‑TGCAGCCAA‑TCAAA‑G‑GTGTAAA‑TTC‑CCGCA‑A‑CCGAACCCGGGCGTGCCAACAACCGCT‑TTGAGCG‑ACAGGCTGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑AGTGGTGGTGCCAGCGTTCCTCA‑ACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTggg                                                                                <  2:1078277/231‑1 (MQ=255)                                                                                                                                                                 aGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGGATTCCGCAAATCTTTAACGATGA‑AGTGGTGGTGCCAGCGTTCCTCA‑ACCGTGGCGTTTCGCTGGAAGATGCGCGCGCCTATTCCGtggtgg                                                                                 >  1:11665/1‑238 (MQ=255)                                                                                                                                                                  gggggttaaactggttttCGTTCCTTT‑GCCT‑TGACCCCTA‑ACAAA‑GCGTG‑CAA‑TTC‑CCGCA‑A‑CCGAACCTGGGCGTGCCCACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑AGTGGTGGTGCCAGCGTTCCTCA‑ACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTggg                                                                                <  1:425433/223‑1 (MQ=255)                                                                                                                                                                  cgggggTAAACGTTCTTTCGTCCCTTT‑GCCT‑TGACCCCTT‑ACAAA‑GCGTA‑CAA‑TGC‑CCGCA‑C‑CCGACCCTCGGCGTCCCCATTACGGCC‑TTGACCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑AGTGGTGGTGCCAGCGTTCCTCA‑ACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTggg                                                                                <  1:82580/234‑1 (MQ=255)                                                                                                                                                                     cgGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑AGTGGTGGTGCCAGCGTTCCTCA‑ACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGCAGTGGGccgg                                                                            >  1:615310/1‑236 (MQ=255)                                                                                                                                                                       gtgacggTGCTTTCTTTCCTTT‑GCCT‑TGCCCCCTA‑TCAAA‑GCGTC‑AAA‑TTC‑CCGCA‑C‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑AGTGGTGGTGCCAGCGTTCCTCA‑ACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCtgtg                                                                           <  1:452336/232‑1 (MQ=255)                                                                                                                                                                       gTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑Agtggtgg                                                                                                                                              >  1:769748/1‑172 (MQ=255)                                                                                                                                                                                           tt‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑AGTGGTGGTGCCAGCGTTCCTCA‑ACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTTTCTATTCCCGGcc                                                      >  1:199060/1‑238 (MQ=255)                                                                                                                                                                                                     aCGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑AGTGGTGGTGCCAGCGTTCCTCA‑ACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCtgtgtg                                                                         >  1:898697/1‑212 (MQ=255)                                                                                                                                                                                                     aCGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑AGTGGTGGTGCCAGCGTTCCTCA‑ACCGTGGCGTTTAGCTTGAAGATGCGCGCGACGATTCCGGAGTGGGCTGTGTGGAATTATCTCTTCCCGGCAGAAcagca                                              >  1:649314/1‑235 (MQ=255)                                                                                                                                                                                                      ccccTA‑TAAAG‑GCGTG‑CAA‑TTC‑CCGCA‑C‑CCAAACCCTGGGGGGCCCATAACCGCC‑TGGATCG‑ACACGCCGTGCCTGATGAAACCCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑AGTGGTGGTGCCAGCGTTCCTCA‑ACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACg                                             <  1:419039/237‑1 (MQ=255)                                                                                                                                                                                                       gccccctttaaatgcttccAA‑TTC‑CCGCA‑C‑CCAAACTTGGGCGTCCCCTCTACCCCT‑TTTTCCG‑ACCCGCCTTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑AGTGGTGGTGCCAGCGTTCCTCA‑ACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTa                                               <  2:11665/221‑1 (MQ=255)                                                                                                                                                                                                        cgctaataaa‑GGGTG‑AAA‑TTCCCCGCA‑A‑CCACCCCTGGGTGTCCGCACAACCGCC‑TGGAGCG‑ACGCGCCGTTCCTGATGAAATCCGCCGAAACCGTTCGGTTCG‑GTACCGTTATTCCGCAAATCTTTAACGATGA‑AGTGGTGGTGCCAGCGTTCCTCA‑ACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACg                                             <  2:1034808/232‑1 (MQ=255)                                                                                                                                                                                                        ccTA‑ACAAA‑GGTTG‑AAA‑TTC‑CCGCA‑A‑CCGACCCTGGGCGTGCGCACTAACGCT‑GTGATCG‑ACACGCCGTTCCTGATGAAATCCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑AGTGGTGGTGCCAGCGTTCCTCA‑ACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGc                                           <  1:337011/239‑1 (MQ=255)                                                                                                                                                                                                         ccaacaaaa‑GCGTG‑CAT‑TTC‑CCGAA‑A‑CCGAACCTCGGCGTCCGCACAAACGCT‑TTGACCG‑ACCCGCCGTTCCTGATGAAACCCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑AGTGGTGGTGCCAGCGTTCCTCA‑ACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGc                                           <  1:583464/233‑1 (MQ=255)                                                                                                                                                                                                          tA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑AGTGGTGGTGCCAGCGTTCCTCA‑Acc                                                                                                                           >  1:15207/1‑157 (MQ=255)                                                                                                                                                                                                                      tG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGTTGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCCAATCTTTAACGATGA‑AGTGGTGGTGCCAGCGTTCCCCA‑ACCGCGGCGTTTCGCTGGAAAATGCGCGCGACTATTCCTGAGCGGGCTGTGCGGCATTATCTATTCCCGCACAAACCTACGGCTTGCaggcagt                                >  2:670304/1‑232 (MQ=255)                                                                                                                                                                                                                      tG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑AGTGGTGGTGc                                                                                                                                           >  1:569771/1‑132 (MQ=255)                                                                                                                                                                                                                      tG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑AGTGGTGGTGCCAGCGTTCCTCA‑ACCGTGGCGTTTCGCTGGAAGATGCGTGCGACTATTCCGTAGTGGGCTGTGTGGAATTATATATTCCCGGCGGAAACTACGGCTTGCTTAACAtg                               >  1:131927/1‑238 (MQ=255)                                                                                                                                                                                                                      tG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAACCTTTAACGATGA‑AGCGGTGGTGCCAGCGTTCCTAA‑ACCGTGGCGTTTCGCTGGAGGAGCCGCGCGCCAATCCCGTAGGGGGCGGTGGGGAATTATCTATTCCCGGCAGAACCTCCGCCCTGccaaaaaac                               >  2:559440/1‑231 (MQ=255)                                                                                                                                                                                                                         aaa‑TTC‑CCGCA‑A‑CCGAACCTGGCGGTGCGCACTAACCCT‑TGGATCG‑ACACGCCGTTCCTGAGGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTAGTCCGCAAATCTTTAACGATGA‑AGTGGTGGTGCCAGCGTTCCTCA‑ACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATcg                              <  1:320026/237‑1 (MQ=255)                                                                                                                                                                                                                                   gCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑AGTGGTGGTGCCAGCGTTCCTCA‑ACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCTTGACATCGCGATGTTTa                     >  1:772798/1‑239 (MQ=255)                                                                                                                                                                                                                                     a‑a‑CCAAACCCCGGCCGGCCCATTAACCCC‑TTGAGCG‑CCACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑AGTGGTGGTGCCAGCGTTCCTCA‑ACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAAt                   <  1:977998/239‑1 (MQ=255)                                                                                                                                                                                                                                            aaCCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑AGTGGTGGTGCCAGCGTTCCTCA‑ACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTCTTCCGTAGTGGGCTGTGTGGACTTTTCTATTCCCGGCAGAACCAACGGGTGCAAGGACATCCGGAGGTTTAATttgcg              >  2:1096565/1‑238 (MQ=255)                                                                                                                                                                                                                                            aaCCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑AGTGGTGGTGCCAGCGTTCCTCA‑ACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTATTGGGCGGGGGGGAATTATTTATTCCCGGCGGAACCTACGGCTTGCAGTAAATAGCGAGGTTTAATatggg              >  2:527528/1‑237 (MQ=255)                                                                                                                                                                                                                                              ccTGGGCGTGCGCCCGAACGCC‑TGGATCG‑ACCCGCCGTTCCTGAAGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑AGTGGTGGTGCCAGCGTTCCTCA‑ACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGa            <  1:436345/239‑1 (MQ=255)                                                                                                                                                                                                                                                  ggCGTGCGCACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑AGTGGTGGTGCCAGCGTTCCTCA‑ACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCTTGACTTCGCGGTGTTTAATTTGGTGAAAgg        >  1:969931/1‑238 (MQ=255)                                                                                                                                                                                                                                                        cgcACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCCAATCTTTAACGATGA‑ATGGGTGGGGCCAGCGTTCCTCA‑ACCGTGGCGTTTCCCTGGAAGATGCGCGGGGCTATTCCGTATGGGGCTGGGGGGAATTACCTTTTCCCGGAGGGACCATCGGCTTCCATGGCATCCGGAGTTTTAATTTGGTGaaaagatagga  >  2:7164/1‑231 (MQ=255)                                                                                                                                                                                                                                                        cgcACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑AGTGGTGGTGCCAGCGTTCCTCA‑ACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGCCATCGCGATGTTTAATCTGCTGAAAGTGATGGa  >  1:259697/1‑239 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                   GGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACC‑GGCTATACCGCACTGCTCGGCGGGTTAACCGAGA‑AC‑GGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTT‑GCCT‑TGACGCCTA‑TCAAA‑GCGTG‑CAA‑TTA‑CCGCA‑A‑CCGAACCTCGGCGTGCGCACTAACGCC‑TTGATCG‑ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCG‑GTACCGGTATTCCGCAAATCTTTAACGATGA‑AGTGGTGGTGCCAGCGTTCCTCA‑ACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGA  >  NC_000913/4144851‑4145324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑