BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,428,195	A→C	9.2%	V175G (GTG→GGG)	ytfB ←	OapA family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,428,195	0	A	C	9.2%	343.9 / 5.3	147	V175G (GTG→GGG)	ytfB	OapA family protein
Reads supporting (aligned to +/- strand): ref base A (67/60); new base C (6/12); total (75/72)
Fisher's exact test for biased strand distribution p-value = 1.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 148 total aligned reads displayed.

TCAACCAGCATACGCTTGGCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCCATCGTTTTACCGGGTTCGACACGGTAAGAGCGCCATTGATTATCAATTCCGCTATCTGGCTGGAATGGTTGCGTTTGGGTAGTTTGCGGCTGCTCTTCTGGCTGGCCTTCCTGTATAGGTTCTGG  >  NC_000913/4427960‑4428427

tCAACCAGCATACGCTTGGCCTGGTTTATCACATCAGTATGGTTTCCCCCCAGGCCCTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCAcc                                                                                                                                                                                                                                            <  1:901642/238‑1 (MQ=255)

   aCCAGCATACGCTTGGCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGGGGCTTTTTCCCATc                                                                                                                                                                                                                                         >  1:230542/1‑238 (MQ=255)

                       gTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CCCACCCCTCGCGT‑TTTGGGGGATTTTTACCacagcccccttttgacaatttccc                                                                                                                                                                                                                    >  2:832273/1‑216 (MQ=255)

                       gTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑GCAC‑CACGCCGCTGGCGG‑TCTGGCGGATTTTTTCCATCTGCCCGTTTTGCAAAT‑TATTc                                                                                                                                                                                                                   >  1:997754/1‑239 (MQ=255)

                       gTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCCTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCGGGCGG‑TCTGGGGGAGTTTCACCAACTCCCCGTTTTGaaaacatacc                                                                                                                                                                                                                    >  2:479487/1‑233 (MQ=255)

                       gTTTATCACAGTAGTATTGTTTCCCCCCAGGCCCTGGAAGCCAGACGTAAGGTCATAAAAGAGACAATCCTTAGCGTGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTc                                                                                                                                                                                                                   <  2:668926/239‑1 (MQ=255)

                         ttttcccttCAGTATGGTTTCCCCCCCGGCACGGGAAGCCAGGCGTAAGGGCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAg                                                                                                                                                                                                                 <  2:389109/231‑1 (MQ=255)

                                 tCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTa                                                                                                                                                                                                         >  1:452688/1‑238 (MQ=255)

                                 tCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTa                                                                                                                                                                                                         >  1:389109/1‑238 (MQ=255)

                                 tCAGAATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGCCGGGTCAACAAGACCCCGTGTTTATTCCCGCTTACACCGGGTAACCCCG‑GCAC‑CCACCCGCCGACGTCTCGGTCGG‑TTTTCACCAACTTCCCCTTTTTCCAAA‑TACTCACCGG‑CTTa                                                                                                                                                                                                         >  2:753625/1‑238 (MQ=255)

                                  cAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACACTCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAACACTGCCTTCCGGTTGGCGGGTGCACCGGCCCTGTTGATTATTACCCCTTACAATGGGTAAGCGCGCTCCC‑CCCCCCCCGGGC‑T‑TTTGCCGGATTTTTCCCTGCACCCCGCTTTACAAAA‑TACTCAGccgggact                                                                                                                                                                                                         >  2:393364/1‑231 (MQ=255)

                                  cAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGGATCAATGGTTAAGCCCG‑TCAC‑CACACCCCTGCAGT‑TCTGGGGGATTTTCACCACTTCCCCGCTTTTCTAAT‑TATTCACCGG‑Ctttc                                                                                                                                                                                                        >  1:693478/1‑236 (MQ=255)

                                  cAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCCGTTGCTTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCCCGGGCGT‑TCTGGCGGCTTTTTACCATCCGGCCGCTTTGCAAATCTAC‑CACCGG‑CTTAc                                                                                                                                                                                                        >  2:953927/1‑238 (MQ=255)

                                    ttttGGTTTCCCCCCAGCGCCGGGAAGTCCGAGGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGGCCCTTTATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCcg                                                                                                                                                                                                     <  2:262267/236‑1 (MQ=255)

                                     tATGGTTTCCCCCCAGGCCCTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCcg                                                                                                                                                                                                     <  1:757072/238‑1 (MQ=255)

                                     gaTGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTGAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCa                                                                                                                                                                                                                                              <  1:588798/198‑1 (MQ=255)

                                          tttCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGACTAACACTGCCATCCGGCTGGCGGGGGAACAGGAACCGTTGATTATTCCCCCTATCAATGGGTACGACCG‑CCAC‑CACCCCCCCGGCGA‑TGTGGCGGGTTTTCACCATCTGCCCGTTTTGCAAAC‑TTATCCGCGG‑Ctcaacccccccc                                                                                                                                                                                                >  2:357351/1‑227 (MQ=255)

                                          tttCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTCGTGATTATTCCCCGTATCAATGGTTAAGCCCC‑TCAC‑CACGCCGCCTGCGT‑TCTGGCGCGATTTCACCCTCGTCCCCCTTTGCACAA‑TACCCACCGGCCTTAcccccccc                                                                                                                                                                                                 >  2:414363/1‑233 (MQ=255)

                                          gttCACCCCAGGCACGGGAAGCCAGACGTAAGGGCAGCAAAGAGACAATCCTTAGCTGGCTTTGGGTTTACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGtt                                                                                                                                                                                                                                 <  1:987900/206‑1 (MQ=255)

                                            tCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGTTTGAACCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TTTGGCGGATTTTCACCATCTGCCCGATTTTCCAAT‑TACTCAGCGG‑CTTACCCCGG‑CCtttt                                                                                                                                                                                            >  1:462116/1‑237 (MQ=255)

                                            tCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAAACGGGCCTGGTGCTTATTCCCCGTCTCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGGTTTGCAAAT‑TGCTCAACGG‑CTTACCCGCG‑CCCTc                                                                                                                                                                                             >  2:770364/1‑238 (MQ=255)

                                            tCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGAGCACGCATAAAACTGCCATCCGGCTGGCGGGGGAACAGGACCTGTTGATTATTCCCCGTGTCACTGGTTAACCCCG‑CCAC‑CACGCCCCTGGCGT‑TTTAGCGGATTTTTACCCTCTCCCCCCTTTGGCAAT‑TAGTCACCAG‑CTTACCCCCG‑cccct                                                                                                                                                                                             >  2:595784/1‑235 (MQ=255)

                                             cACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGGGAACAGGACCTGTTGATTATTCCCCCTATCAATGGGTAAGCCCC‑GCAC‑CACGCCGCTGGCGT‑TCTGGCGGAATTTTACCCTCTCCCCCTTTTGCAAAA‑TACCCAGCGG‑GTTACCCGCGACCTTc                                                                                                                                                                                             >  2:294896/1‑238 (MQ=255)

                                              aCCCCAGGCCCTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGAGCCTTTGATTATTCCCCGCATCAATGGTTAAGCCCG‑TCAC‑CCCGCCGCTGGCGT‑TCTGGCGGGTTTTTTCCATCTTCCCGCTTTGCCAAT‑TACTCCCGGG‑CTTTCCCCCG‑CCTTCTa                                                                                                                                                                                           >  2:1080236/1‑238 (MQ=255)

                                               ccccAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTAc                                                                                                                                                                                          >  1:293006/1‑238 (MQ=255)

                                                 ccAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAACCTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTg                                                                                                                                                                                       <  1:479487/239‑1 (MQ=255)

                                                 ccAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTg                                                                                                                                                                                       <  1:129557/239‑1 (MQ=255)

                                                   aGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCaaa                                                                                                                                                                                                                          >  1:655900/1‑205 (MQ=255)

                                                   aGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCaaa                                                                                                                                                                                                                          <  2:655900/205‑1 (MQ=255)

                                                    ggCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTCCCGCGAACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCCTATCAATGGTTAAGCCCG‑TCAC‑CACCCCGCTGGCGT‑TCTGGCGGATTTTCCCCATCTCCCCGTTTTCCAAAA‑TAACCAACCG‑CTTAACcgccgctttcaccccgc                                                                                                                                                                                      >  2:416542/1‑224 (MQ=255)

                                                    ggCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACCGGGCCCGGTGATTATTCCCCGGATCAATGGGTAAGCCCG‑TCACACCCGCC‑CTGGGGT‑TCTTGCGGCTTTTTAACCTCTGCCCGTTTTGCAAAT‑TACTCAGCAG‑CGTACCCCCG‑CCCTCTACCtcgcc                                                                                                                                                                                    >  2:680835/1‑235 (MQ=255)

                                                    ggCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGGACTGTTGATTATTCCCCGTATCAAAGGTTAAGCCCT‑GCAC‑CACCCCGCCGGGCT‑TCTGGCGGGTTTTTACCATCTGCCCGCTTTGCAAAT‑TACCCAACGG‑CCTACCCCCC‑CCTCTTAccctgg                                                                                                                                                                                     >  2:997389/1‑234 (MQ=255)

                                                    ggCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTTACCATCTGCCCGTTTTGCAAAT‑TACTCAACGG‑CTTACCCGCC‑CCTTCTACCTgcg                                                                                                                                                                                     >  1:1047035/1‑238 (MQ=255)

                                                    ggCACTGGAAGCCAGACGTAAGCTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAA‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATATGCCCGTTTTGCAACT‑TACTCAGCGG‑CTTAACCCCG‑CCCTCTcccctcg                                                                                                                                                                                     >  2:319744/1‑234 (MQ=255)

                                                      ccttGGAAGCCATCCGTAAGGTCACCAAAGGGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCAGCTACCGCGCACGAATAAACCTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGcc                                                                                                                                                                                   <  2:69270/235‑1 (MQ=255)

                                                      cACTGGAAGCCAGACGTAAGGTCAACAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGcc                                                                                                                                                                                   <  1:624600/238‑1 (MQ=255)

                                                       aCTGGAAGCCAGACGTAAGGTCAACAAAGAGACAATCCTTAGCTGGCTTTGGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCa                                                                                                                                                                                  <  1:294896/238‑1 (MQ=255)

                                                        cTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCAt                                                                                                                                                                                 <  1:198399/238‑1 (MQ=255)

                                                            aaGCCAGACGTAAGGTCATCAAAGAGACAATCCTTTGCTGGCTTTTGGGTGACCCTTCATCTCCCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCa                                                                                                                                                                             <  2:885236/238‑1 (MQ=255)

                                                             aGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATa                                                                                                                                                                           <  1:25147/239‑1 (MQ=255)

                                                              gCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTTTACCTGCGCCATTGCATAg                                                                                                                                                                          >  1:69270/1‑239 (MQ=255)

                                                                    cGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACAt                                                                                                                                                                      <  1:953927/237‑1 (MQ=255)

                                                                          gTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGGATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGGTTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCC‑CCTTCTACCTTCGCCCtt                                                                                                                                                                                >  1:415961/1‑221 (MQ=255)

                                                                           tcatcaAAGAAGAAAACCTTAGGTGGTTTTTGGTTGCCCCTTCCACTACCGGGCCCGAATAAAACGGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCTCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCg                                                                                                                                                              <  2:972903/238‑1 (MQ=255)

                                                                            catcaaaagatcAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCCCGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCggg                                                                                                                                                            <  1:680835/228‑1 (MQ=255)

                                                                             atcaAAGAGCCAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCggg                                                                                                                                                            <  1:665679/238‑1 (MQ=255)

                                                                             atcaAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGGGAACAGGACCTGTTGATTATTCCCCGTATCAAAGGTTAAGCCCG‑TCCC‑CACGCCCCCTGCCT‑TCTGGCGGATTTTTACCATCTCACCGTTTTGCCAAT‑TACCCACCGG‑CTTACCCGCC‑CCTTCTACCTTCGCCACTGCATaaaacacgcgagggggc                                                                                                                                                           >  2:665679/1‑223 (MQ=255)

                                                                                          tCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGt                                                                                                                                                                  <  1:804164/219‑1 (MQ=255)

                                                                                          tCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCC‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGTGGTcgc                                                                                                                                              >  1:515847/1‑239 (MQ=255)

                                                                                                 gtGGTTTTTGGTTGCCCCTTCATCCCCCCCGCACGAAAAAAACTGGCAGCCGGCTGGGGGGGGAACAGTCACTGTTGATTATTCCCCGTATCAATGGTTAATCCCG‑TCCC‑CCCGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAg                                                                                                                                      <  2:415403/238‑1 (MQ=255)

                                                                                                    gCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCcg                                                                                                                                                                                                     >  1:1071020/1‑175 (MQ=255)

                                                                                                    gCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCcg                                                                                                                                                                                                     <  2:1071020/175‑1 (MQ=255)

                                                                                                    gCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCC‑CCTTCTACCTGCGCCATTGCCTAGACATCGGTAGCCGGCCGTCCCTGGCCCC‑GGACAAGCt                                                                                                                                    >  1:952901/1‑238 (MQ=255)

                                                                                                         tGGTTGACCTTTCACTCACCCGGCCCGAATAAAACTGCCACCCGGCTGGGGGGGGAACAGGCCCGTTTGATTTTTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCCa                                                                                                                              <  2:33564/239‑1 (MQ=255)

                                                                                                         tGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCCa                                                                                                                              <  1:134439/239‑1 (MQ=255)

                                                                                                               aCCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCCATGGTTAAGCCCG‑TCAC‑CACGCCCCTGGCGT‑TCTCGCGGATTTTCACAATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CCTTCCCCCC‑CCCTCCACCTCCCCCAATTCCCATCCATCGGCGGCGGGCAGCCCGCGTTCCC‑CGAACacccagcccaatcgtt                                                                                                                        >  2:47994/1‑224 (MQ=255)

                                                                                                                 ctctcattcccccccgactaaTAAAACTGCCCTCCGGCGGGGGGGTGAACAGGACCTTGTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCCATCGTTTTa                                                                                                                      <  2:452688/220‑1 (MQ=255)

                                                                                                                    tCATCTACCGCGCACGAATAAACCTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCGGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCCa                                                                                                                              <  1:821276/228‑1 (MQ=255)

                                                                                                                     cATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTTTACCTGCGCCCTTGCATAGACCTCGGTAGCGGGCAGTCCGCGGTCCCGGGAACA‑CTGGCCCATCCGTTTACCgg                                                                                                                  >  1:504372/1‑239 (MQ=255)

                                                                                                                           ccggccACGAAGAAAACGGCCATCCGGCTGGCGGGTGAACAGGACCTGGTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCCATCGTTTTACCGGGTTCGa                                                                                                            <  1:1080236/234‑1 (MQ=255)

                                                                                                                           cccggcACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCCATCGTTTTACCGGGTTCGa                                                                                                            <  1:176427/235‑1 (MQ=255)

                                                                                                                              cgcACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTACCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCCATCGTTTTTCCGGGTTCGacac                                                                                                         >  1:330469/1‑239 (MQ=255)

                                                                                                                                cACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTCACTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACCCCGCTTGCGT‑TCTGGCGGACTTTTCCCATCTGCCCCTTTTGCCAAT‑TACCCAGCGG‑CTTTACCCCG‑CACTCTACCTTCGCCACTCCCTATCCATTGCCAACGGGCCGTCCCGGGTCTC‑GGGACACATCGCCCACCTTTTTACAGGGTTCgcaaacg                                                                                                       >  2:84219/1‑233 (MQ=255)

                                                                                                                                   caattacccTGCCATCCGGCTGGCGGGTGACTAGGACCGGTTGAGTATCCCCCGTACCAATGTTTAAGCCCG‑TCCC‑CCCGCCGCTGGCGT‑TCGGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCCATCGTTTTACCGGGTTCGACACGGTaa                                                                                                    <  2:515847/231‑1 (MQ=255)

                                                                                                                                   caaTAAACCTCCCCCCCGGGTTGCGGGTTAACAGGGCCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑GCCC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATCGCATAGACATCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCCATCGTTTTACCGGGTTCGACACGGTaa                                                                                                    <  2:1047035/238‑1 (MQ=255)

                                                                                                                                           ctccccttcGGCTGGGCGGTGAACAGGGTCGGTTGGTTTTTCCCCGTAACAATGGTTAAGCCCG‑TCAC‑CACGCCGCTTGCCG‑TCTGGCGGATTTTCACCCTCTCCCCGTTTTGCAAAT‑TACCCATCGG‑CTTACCCGTG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCCATCGTTTTACCGGGTTCGACACGGTAAGAGCGCCa                                                                                            <  2:803047/231‑1 (MQ=255)

                                                                                                                                             gCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCa                                                                                                                                                                                  >  2:318668/1‑152 (MQ=255)

                                                                                                                                               cATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGc                                                                                                                                                                                                                                      <  2:15188/101‑1 (MQ=255)

                                                                                                                                                 cccGGCTGGTTGGTGAACAGGCCCTTGTGATTATGCCCCGTATCAATGGTTAAGCCCG‑GCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCCATCGTTTTACCGGGTTCGACACGGTAAGAGCGCCATTGAtt                                                                                      <  1:626235/238‑1 (MQ=255)

                                                                                                                                                   cGGCTGGCGGGTTAACAGGACCTGTTGATTATGCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCCATCGTTTTACCGGGTTCGACACGGTAAGAGCGCCATTGATTAt                                                                                    <  1:770364/239‑1 (MQ=255)

                                                                                                                                                            ggTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CCCGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCCATCGTTTTACCGGGTTCGACCCGGTAAGAGCGCCATTGATTATCAATTCCg                                                                            <  1:321345/238‑1 (MQ=255)

                                                                                                                                                                aaCAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGtttt                                                                                                                                                                                                                               <  2:888778/91‑1 (MQ=255)

                                                                                                                                                                aaCAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGtttt                                                                                                                                                                                                                               >  1:888778/1‑91 (MQ=255)

                                                                                                                                                                     gACCTGTTGATTATTCCCCGTATCATTGGTTCAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGCCGGATTTTCCCCATCTGCCCGTTTTGCAAAT‑TCCTCCGCGG‑ATTACCCCCG‑CCTTCTACCTGCGCCCTTGCATAGCCATCCCTACCCGCCAGTCCGCGTCCAC‑GGATCCCCCCCGCCATCATTTTACCGGGTTCGACACGGTACGAGCGCACTCGCTTATCAATTCCGcatctcggcg                                                                  >  2:628006/1‑230 (MQ=255)

                                                                                                                                                                           ttGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGt                                                                                                                                                    >  1:200757/1‑152 (MQ=255)

                                                                                                                                                                           ttGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGt                                                                                                                                                    <  2:200757/152‑1 (MQ=255)

                                                                                                                                                                                     cccGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCCATCGTTTTACCGGGTTCGACACGGTAAGAGCGCCCTTGCTTATCAATTCCCCTTTTTGGCGGGAAGGGtggggtggg                                                  >  2:569866/1‑231 (MQ=255)

                                                                                                                                                                                     cccGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCCATCGTTTTACCGGGTTCGACACGGTAAGAGCGCCATTGATTATCAATTCCCCTATCTGGCTGGAATGGTTGCGTTTg                                                  >  1:797818/1‑239 (MQ=255)

                                                                                                                                                                                       cttATCATTGTTTAAGCCCG‑TTGC‑AGCGCCGGTGGCGT‑TTGGGCGGGTTTTCCACAGCTGGCCGTTTTGCAAAT‑GTCTCGTGGG‑CTTACCCGGG‑CCTTCTACCGGCGCCATTGCATAGACATCGGTAGCGGGTGGTCCGTGGTCGC‑GGAACAGCTGCGTCATCGTTTTACAGGGTTCGACACGGTAAGAGCGCCATTGACTATCAATTCCGCTATCTGGCTAGAATGGTTGCGTTTggg                                                <  2:680214/237‑1 (MQ=255)

                                                                                                                                                                                       cttATCATTGTTTAACGCCC‑TCCC‑CCCGCCGGTGGCGT‑TCTGGCGGATTTTCCCCACCTGCCCGGTTTGCAAAG‑TACTCAGGGG‑CTTACCCGCG‑CCTTCTGCCTGCGCCATTGCATAGACGTCGGTAGCGGGCAGGCCGTGGTCGC‑GGAACAGCTGCGCCATCGTTTTACCGGGTTCGACACGGTAAGAGCGCCATTGATTATCAATTCCGCTATCTGGCTGGAATGGTTGCGTTTggg                                                <  2:230542/237‑1 (MQ=255)

                                                                                                                                                                                        gTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGTGGTCCC‑GGAACAGCTGCGCCATCGTTTTAACGGGTTCGACACGGTAAGAGCGCAATTGATTATCAATTCAGCTATCGGGCGGGAATGGTTGCGTTTgggg                                               >  2:128126/1‑238 (MQ=255)

                                                                                                                                                                                         tATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCCATCGTTTTACCGGGTTCGACACGGTAAGAGCGCCATTGATTATCAATTCCGCTATc                                                                       >  1:155871/1‑214 (MQ=255)

                                                                                                                                                                                                    aaGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCCATCGTTTTACCGGGTTCGACACGGTAAGAGCGCCATTGATTAt                                                                                    >  2:298080/1‑190 (MQ=255)

                                                                                                                                                                                                       cccG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCCATCGTTTTACCGGGTTCGACACGGTAAGAGCGCCATTGCTTATCAATTCCGCTATCTGGCTGGAATGGTTGCGTTTGGGTAGTTTGCGgctgc                                  >  1:1045244/1‑237 (MQ=255)

                                                                                                                                                                                                            tCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCCATCGTTTTACCGGGTTCGCAACGGTAAGAGCGCCATTGATTATCAATTCCGCTATCTGGCTGGAATGGTTGCGCTTGGGTAGTTTGCGGCTCctct                               >  1:967670/1‑236 (MQ=255)

                                                                                                                                                                                                            tCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCCATCGTTTTACCGGGTTCGACACGGTAAGAGGCCCATTGCTTTTCAATTCCGTTCTCTGGCTGGAATGGTGGCGTTGGGGAAGttgttgctggactc                               >  2:1025182/1‑224 (MQ=255)

                                                                                                                                                                                                               c‑cacGCCCGTGGCGT‑TCTGGCGGATTTTCACCATCCGCCCGTTTTGCAAAT‑TACCCAGCGG‑CTTCCCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCCATCGTTTTACCGGGTTCGACACGGTAAGAGCGCCATTGATTATCAATTCCGCTATCTGGCTGGAATGGTTGCGTTTGGGTAGTTTGCGGCTGCTCTTctggc                         <  1:416542/239‑1 (MQ=255)

                                                                                                                                                                                                                cccccccgcTGGCGT‑GTTGGCGGATTTTCACCACCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCCATCGTTTTACCGGGTTCGACACGGTAAGAGCGCCATTGATTATCAATTCCGCTATCTGGCTGGAATGGTTGCGTTTGGGTAGTTTGCGGCTGCTCTTctggc                         <  2:952901/234‑1 (MQ=255)

                                                                                                                                                                                                                   cgccgcTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCCATCGTTTTACCGGGTTCGACACGGTAAGAGCGCCATTGCTTATCAATTCCCCTCTCTGGCTGGGATGGTTGCGTTTGGGAAGTTTGCGGTGGCCCTTttggctg                       >  2:536281/1‑238 (MQ=255)

                                                                                                                                                                                                                      cggtGGCGT‑TCTGGCGGATTTTCACCATCGGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCGTTGCATAGACATCGGTAGCGGGCAGGCCGTGGTCGC‑GGAACAGCTGCGCCATCGTTTTACCGGGTTCGACACGGTAAGAGCGCCATTGATTATCAATTCCGCTATCTGGCTGGAATGGTTGCGTTTGGGTAGTTTGCGGCTGCTCTTCTGGCTGGcc                    <  1:753625/235‑1 (MQ=255)

                                                                                                                                                                                                                      cgcTGGCGT‑TCTGGCGGATTTTCCCCATCTGCCCGTTTTGCAAAT‑TACCCAGCGG‑CTTACCCGGG‑CCTTCTACCTGCGCCATTGCATAGACGTCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCCATCGTTTTACCGGGTTCGACACGGTAAGAGCGCCATTGATTATCAATTCCGCTATCTGGCTGGAATGGTTGCGTTTGGGTAGTTTGCGGCTGCTCTTCTGGCTGGcc                    <  1:595784/238‑1 (MQ=255)

                                                                                                                                                                                                                      cgcTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCCATCGTTTTACCGGGTTCGACACGGTAAGAGCGCCATTGATTATCAATTCCGCTATCTGGCTGGAATGGTTGCGTTTGGGTAGTTTGCGACTGTTCTTCTGGCTGGCCt                   >  1:1040529/1‑239 (MQ=255)

                                                                                                                                                                                                                       gcTGGCGT‑TCCGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TCCCCAGCGG‑GTTACCCGCG‑CCTTCTGCCTGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCCATCGTTTTACCGGGTTCGACACGGTAAGAGCGCCATTGATTATCAATTCCGCTATCTGGCTGGAATGGTTGCGTTTGGGTAGTTTGCGGCTGCTCTTCTGGCTGGCCtt                  <  1:393364/239‑1 (MQ=255)

                                                                                                                                                                                                                              t‑tCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCCATCGTTTTACCGGGTTCGACACGGTAAGAGCGCCATTGATTATCAATTCCGCTATCTGGCTGGAATGGTTGCGTTTGGGTAGTTTGCGGCTGCTCTTCTGGTTGGCCTTCCttgtt           >  1:664475/1‑235 (MQ=255)

                                                                                                                                                                                                                               ttttGGCGGTTTTTCCCCATTTGCCCGTTTTGCAAAT‑TTCTCAGCGG‑CTTACCCGCC‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCCATCGTTTTACCGGGTTCGACACGGTAAGAGCGCCATTGATTATCAATTCCGCTATCTGGCTGGAATGGTTGCGTTTGGGTAGTTTGCGGCTGCTCTTCTGGCTGGCCTTCCTGtat           <  2:465071/236‑1 (MQ=255)

                                                                                                                                                                                                                                tttgtgtgtttttCCCCTTCTGCCCGTTTTGCAAAT‑TGCTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACACCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCTATCGTTTTACCGGGTTCGACACGGTAAGAGCGCCATTGATTATCAATTCCGCTATCTGGCTGGAATGGTTGCGTTTGGGTAGTTTGCGGCTGCTCTTCTGGCTGGCCTTCCTGtat           <  2:189246/229‑1 (MQ=255)

                                                                                                                                                                                                                                ttgggCGGATTTTCCCCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCGGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCCATCGTTTTACCGGGTTCGACACGGTAAGAGCGCCATTGATTATCAATTCCGCTATCTGGCTGGAATGGTTGCGTTTGGGTAGTTTGCGGCTGCTCTTCTGGCTGGCCTTCCTGtat           <  1:312977/235‑1 (MQ=255)

                                                                                                                                                                                                                                tCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TCCTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCTCCATTGCATAGACATCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCCATCGTTTTACCGGGTTCGACACGGTAAGAGCGCCATTGATTATCAATTCCGCTATCTGGCTGGAATGGTTGCGTTTGGGTAGTTTGCGGCTGCTCTTCTGGCTGGCCTTCCTGgat           <  1:552365/238‑1 (MQ=255)

                                                                                                                                                                                                                                         ttttCCCCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCCATCGTTTTACCGGGTTCGACACGGTAAGAGCGCCATTGATTATCAATTCCGCTTTCTGGCTGGAATGGTTGCGTTTGGGTAGTTTGCGGCTGCTCTTCTGGCTGGCCTTCCTGTATAGGTTCTgg  <  1:569866/238‑1 (MQ=255)

                                                                                                                                                                                                                                         ttttCCCCATCTGCCCGTTTTGCAAAT‑TACCCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCCATCGTTTTACCGGGTTCGACACGGTAAGAGCGCCATTGATTATCAATTCCGCTATCTGGCTGGAATGGTTGCGTTTGGGTAGTTTGCGGCTGCTCTTCTGGCTGGCCTTCCTGTATAGGTTCTgg  <  1:309169/238‑1 (MQ=255)

TCAACCAGCATACGCTTGGCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCG‑TCAC‑CACGCCGCTGGCGT‑TCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAAT‑TACTCAGCGG‑CTTACCCGCG‑CCTTCTACCTGCGCCATTGCATAGACATCGGTAGCGGGCAGTCCGTGGTCGC‑GGAACAGCTGCGCCATCGTTTTACCGGGTTCGACACGGTAAGAGCGCCATTGATTATCAATTCCGCTATCTGGCTGGAATGGTTGCGTTTGGGTAGTTTGCGGCTGCTCTTCTGGCTGGCCTTCCTGTATAGGTTCTGG  >  NC_000913/4427960‑4428427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`