BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,522,784	T→G	16.0%	T438P (ACC→CCC)	yjhG ←	putative dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,522,784	0	T	G	16.0%	223.0 / 3.2	112	T438P (ACC→CCC)	yjhG	putative dehydratase
Reads supporting (aligned to +/- strand): ref base T (36/50); new base G (22/2); total (60/52)
Fisher's exact test for biased strand distribution p-value = 1.36e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

GGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAATGACCAGAATAT‑CGCCCGCCTTGATCTTGTCATGT‑TTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCT‑CCCACCAGTC‑GAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAG  >  NC_000913/4522548‑4523019

ggTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAATGACCAGAATAT‑CGCCCGCCTTGATCTTGTCATGT‑TTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGa                                                                                                                                                                                                                                               <  1:1048028/239‑1 (MQ=255)

gatAACCGGGTAGTTTTCTTCCATCCCGTTACGTGAAGGTCAAACGCCAATAATGACCAGAATAG‑CGCCCGCCTTGATCTTGTCATGT‑TTGATATCGTAAATCGCACTTTTCTCGGACAGATAAGCCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGa                                                                                                                                                                                                                                               <  1:591746/237‑1 (MQ=255)

    agagaccgggTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAATGACCAGAATAT‑CGCCCGCCTTGATCTTGTCATGT‑TTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGt                                                                                                                                                                                                                                           <  1:187613/231‑1 (MQ=255)

    aCCTGGTTGGTTTCTTCCACCCCTGTCCCGTAAGTTCCAACGTCAATAATGCCCAG‑ATATCCCCCCTCCTTGATCTTGTCATGG‑TTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGt                                                                                                                                                                                                                                           <  2:827373/239‑1 (MQ=255)

    aCCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAATGACCAGAATAT‑CGCCCGCCTTGATCTTGTCATGT‑TTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGGGATAGt                                                                                                                                                                                                                                           >  1:558848/1‑239 (MQ=255)

         gTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAATGACCAGAATAT‑CGCCCGCCTTGATCTTGTCATGT‑TTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGAAATAGATACCTT‑GCTCATCAATCATCGAGAGGTCAATGGCGATGGATTTTATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACAGGGAAGGGGATAGTTgggg                                                                                                                                                                                                                                      >  1:519926/1‑239 (MQ=255)

                tcttcCATCCCTGTACCTGAAGGTCCAACGCCAATAATGACCAGAATAT‑CGCCCGCCTTGATCTTGTCATGT‑TTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGGGATAGTTGAGGTTAATc                                                                                                                                                                                                                                >  1:1034032/1‑238 (MQ=255)

                tcttcCATCCCTGTACCTGAAGGTCCAACGCCAATAATGACCAGAATAT‑CGCCCGCCTTGATCTTGTCATGT‑TTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGCGGCAATATTG‑CCCACCAGGAAGGGGTTGGTTGAGGTTatt                                                                                                                                                                                                                                 >  1:39744/1‑235 (MQ=255)

                   tcccccccTTTCCCTGAAGGTCCAACGCAAAAAATGCCCAAAATTT‑CGCCCGTCTTGATCTTGTCATGT‑TTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCcgcg                                                                                                                                                                                                                            <  2:649573/234‑1 (MQ=255)

                   aaggagcaggttccTGAAGGTCCAACGTCAATAATGACCAGAATTC‑CGCCCGCCTTGATCTTGTCATGT‑TTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCcgcg                                                                                                                                                                                                                            <  1:334455/227‑1 (MQ=255)

                        cccTTTACCTGAAGGTCCAACGCCAATAATGACCAGAATTT‑CGCCCGCCTTGATCTTGTCATGT‑TTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGt                                                                                                                                                                                                                                           <  1:808961/219‑1 (MQ=255)

                            tatactgatgttcaaactgcAATAAGTACAAGAAGAT‑CGCACGCCTTGCAGTAGTC‑TGTGTTGATAGCGATAATCGAACTTTTCACGGACCGGTAACCAGTCACCACACTGTTATGGTAATAGATGCCTT‑GCTCATCAATCAACGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCGGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCTCGCTTttgc                                                                                                                                                                                                                   <  2:519926/220‑1 (MQ=255)

                            gTACCTGAAGGTCCAACGCCAATAATGACCAGAATAT‑CGCCCGCCTTGATCTTGTCATGT‑TTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCCCCGGGAAGGTGTTAGTTGAGGTTAATCCGCCCGCtttt                                                                                                                                                                                                                     >  1:802213/1‑237 (MQ=255)

                                  gAAGGTCCAACGCCAATAATGACCAGAATAT‑CGCCCGCCTTGCTCTTGTCATGT‑TTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGCGGGGTCAATGGCGGTGGGTTTGATCACCGACACCTCTTGAGCCCATATT‑‑CCCGCCGGGAAGGGGTGAGGTGAGGTTAAAcccgtctctttatacaatca                                                                                                                                                                                                             >  2:808961/1‑221 (MQ=255)

                                  gAAGGTCCAACGCCAATAATGACCAGAATAT‑CGCCCGCCTTGATCTTGTCATGT‑TTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTTATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGGAGGGGGTAGGTGAGGTTAATCCGGGCGCTTTTGGTtgctg                                                                                                                                                                                                             >  2:567687/1‑239 (MQ=255)

                                  gAAGGTCCAACGCCAATAATGACCAGAATAT‑CGCCCGCCTTGATCTTGTCATGT‑TTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTTATCACCGA‑AACGTCTGGCGCAATATTG‑CCCCCAGGGGAGGGTGTTGTTGGTGTTAATCCGCGCGCCTtttctttgtg                                                                                                                                                                                                             >  2:912186/1‑235 (MQ=255)

                                  gAAGGTCCAACGCCAATAATGACCAGAATAT‑CGCCCGCCTTGATCTTGTCATGT‑TTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTTGCGCAAAATTG‑CCCCCCGGGGAGGGGATAGTTGAGGTTAATCCGCGCGCCTttgcttcgtc                                                                                                                                                                                                             >  1:616053/1‑235 (MQ=255)

                                   aaGGTCCAACGCCAATAATGACCAGAATAT‑CGCCCGCCTTGATCTTGTCATGT‑TTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATTACCGA‑ACCTTCTGGCGCACAATTG‑CCCACCGGGAAGGAGATAGTTGAGGTTAATCCGCGCGCTTTTTCTtgctgc                                                                                                                                                                                                            >  1:360351/1‑239 (MQ=255)

                                     ggTCCAACGCCAATAATGACCAGAATAT‑CGCCCGCCTTGATCTTGTCATGT‑TTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGGTTTTTTTACCGA‑ACCTTCTGGCGCAATATTC‑TCCACCGGGAAGGGGGTAGTTGGGGTTAATCCGCGCGCtttttttttccgtgg                                                                                                                                                                                                          >  2:154375/1‑228 (MQ=255)

                                       tCCAACGGCAATAATGACCAGAATAT‑CGCCCGCCTTGATCTTGTCATGT‑TTGATATCGTAAATCGCACTTTTCGCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGga                                                                                                                                                                                                         <  1:122700/238‑1 (MQ=255)

                                       tCCAACGCCAATAATGACCAGAATAT‑CGCCCGCCTTGATCTTGTCATGT‑TTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTTGTCACCGA‑ACCTTCTGTCGCAATATTG‑ACCCCCGGGAAGGGGGAAGTTAGAGTTAATCCCCCCGCtttttttctttttcttt                                                                                                                                                                                                        >  2:334455/1‑226 (MQ=255)

                                                 ataatGACCAGAATAT‑CGCCCGCCTTGATCTTGTCATGT‑TTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTTGCGCAATATTG‑CCCACCGGGAAGGGGATAGTTGAGGTTAATCCCCCCGCTTTTGCTTCCTGCGGAGACATGAT‑c                                                                                                                                                                                               >  1:1019000/1‑237 (MQ=255)

                                                 ataatGACCAGAATAT‑CGCCCGCCTTGATCTTGTCATGT‑TTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATAATCGACGGGCCAATGGCGGTGGATTTTATCACCCC‑ACCTACTGCCGTCCTATTGCCCCACGGGGAAGG‑GTTCGTTTAGGTTAATCCGCCGGCCTTTGCTTGTTTCgggaccctgagca                                                                                                                                                                                               >  2:112139/1‑227 (MQ=255)

                                                                 tcccccgccTTGTTCTTGTCATTT‑TTGTGACCGTAAATCGCACTTTACCCGGACAGATAATCCCTCGCTACACCTTTATGGTAATAGATGCCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTTCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGAt                                                                                                                                                                              <  2:978077/236‑1 (MQ=255)

                                                                  cgcccgccTTGATCTTGTCATGT‑TTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCCCCGGGAAGGGGATAGTTGAGGTTAAt                                                                                                                                                                                                                                 >  1:866122/1‑188 (MQ=255)

                                                                  cgcccgccTTGATCTTGTCATGT‑TTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGAGGATTTGATCACCGA‑ACCTTCTGTCGCAATATTG‑CCCACCGGGAAGGGGATAGTTGAGGTTAATCCGCGCGCTTTTGCCTTCTGCGGCATAATGGT‑CACTTCGGCAGCGTTGagt                                                                                                                                                                             >  1:88631/1‑237 (MQ=255)

                                                                    cccgccTTGATCTTGTCATGT‑TTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTGGATTTg                                                                                                                                                                           >  1:349318/1‑239 (MQ=255)

                                                                       gccTTGATCTTGTCATGT‑TTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGGTTTTGTTACCAC‑ACCTTCCGGCCGAATATTT‑CCCACCGGGAAGGTGGTGATTTGGGTTAAATCGCGGGCTTTTTCTTGCTGCGGGGACATGGTACAC‑TCGTCAGCGGTGTtttttt                                                                                                                                                                         >  2:1106243/1‑238 (MQ=255)

                                                                       gccTTGATCTTGTCATGT‑TTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGGTTTGATCACCGA‑ACCTTCTTGCGCAATATTG‑TCCACCGGGAAGGGGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATTAT‑CACTTCGTCAGCGATGATTTGtt                                                                                                                                                                         >  1:998155/1‑238 (MQ=255)

                                                                       gccTTGATCTTGTCATGT‑TTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGGGATAGTTGAGGTTAATCCGCCGGCTTTTCCTTGCTCCGGGGACCTGAT‑CACTTAGTCCGCGTTGGTTTGtt                                                                                                                                                                         >  1:448301/1‑238 (MQ=255)

                                                                                ttGTCATTT‑TTGATATCGTAAATCGCCCTTTTCTCGGACAGATAAACCTTCGCCACCCCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAg                                                                                                                                                              <  1:1106243/239‑1 (MQ=255)

                                                                                  gTCATGT‑TTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCCCCGGGAAGGGGGTAGTTGGGGGTAATCCGGCCGCTTTTCTTTGCTGGGGGGGCAGTAT‑ACCTTTGTCACCGGTGATTTGTTCCTgttggggaa                                                                                                                                                             >  2:224596/1‑231 (MQ=255)

                                                                                     aTGT‑TTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGa                                                                                                                                                         <  1:224596/239‑1 (MQ=255)

                                                                                     aTGT‑TTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGa                                                                                                                                                         <  1:154375/239‑1 (MQ=255)

                                                                                        t‑ttGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGGGTGAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGCACGCGTTGATTTGTTCCCTTT‑CGGGCAGGGGtt                                                                                                                                                      >  1:267391/1‑239 (MQ=255)

                                                                                                     aaTCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGAt                                                                                                                                                                              <  1:282000/204‑1 (MQ=255)

                                                                                                        cGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATGCCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGAc                                                                                                                                       <  1:912186/239‑1 (MQ=255)

                                                                                                         ccctttttCCGGGACAGATAAACCTTCGACACCCGTTTATGGTAATAGATGCCTTGGCACA‑CAATCATCGAGGGGTCAATGGGGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACg                                                                                                                                      <  2:1056448/235‑1 (MQ=255)

                                                                                                           aCTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑AACTTCTGGCGCAATATTG‑CCCACCGGGGAAGTGATAGTTTAGGTTAATCCGCGCGCTTTTGCTTTCTGCGGAGCCATGAT‑CACTTCGTCCGCGCTGATTTGTTCCTGAT‑CCAGCAGAAGTTGCTTGACCCGCTGAGGcg                                                                                                                                    >  1:200277/1‑238 (MQ=255)

                                                                                                                 ctcGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATCCCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCgg                                                                                                                              <  1:100717/239‑1 (MQ=255)

                                                                                                                  ttggcaaGATAACCCTTCGCCACACCTTTAAGGGAATAGAGACCTT‑GCGTATTAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGGCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGa                                                                                                                             <  2:164241/233‑1 (MQ=255)

                                                                                                                        aGAAAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCt                                                                                                                        <  1:567687/238‑1 (MQ=255)

                                                                                                                                    gCCACCCCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGa                                                                                                                                                         <  1:375087/193‑1 (MQ=255)

                                                                                                                                    gCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTTATCACCGA‑ACCTTCTTGCGCAATATTT‑CCCACCGGGAAGGGGATAGTTGAGGGTAATCCGCTCGCTTTTGCTTTCTGCGGGGACCTGAC‑TCCTTCGTTAGCGTTGATTTTTTCCTGTT‑CGGGCGGGAGTGTCTTTAACCCCTTCCGCCCTTCGGGGGGTT‑CCCACCAGTccgg                                                                                                          >  2:107681/1‑236 (MQ=255)

                                                                                                                                    gCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGGCATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGCCGCCGTTCGGAGTGTT‑CCCACACGTC‑GAg                                                                                                         >  1:475640/1‑239 (MQ=255)

                                                                                                                                    gCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGAt                                                                                                                                                                              <  2:181776/173‑1 (MQ=255)

                                                                                                                                    gCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTTTTCCTGAT‑CGAGCAGGGGTTGCTTTAACCGCTGAGGCCGTTCTGGGTGCT‑CCCCCCCGTC‑GAg                                                                                                         >  1:1036003/1‑239 (MQ=255)

                                                                                                                                    gCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCACCGTTGATTTGTTCCTGAT‑CGGGCAGGGGTTGCTTGAACCGCTGACGCCGTTCGGGGTGCT‑CCCACCAGTC‑GAg                                                                                                         >  1:575041/1‑239 (MQ=255)

                                                                                                                                     ccACCCCTTTATGGTAATAGATTCCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCT‑CCCACCAGTC‑GAgg                                                                                                        <  1:392071/239‑1 (MQ=255)

                                                                                                                                         acCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGGTCACCGA‑ACCTTCTTGCGCAATATTG‑CCCACCGGGAAGGTGATAGGTTAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCT‑CCCACCAGTC‑GAGGtttt                                                                                                    >  1:50468/1‑239 (MQ=255)

                                                                                                                                               aTGGTAATAGAATCCTT‑GCTCATCAAACAGCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCT‑CCCACCAGTC‑GAGGTTTTCTTTCa                                                                                              <  1:213321/239‑1 (MQ=255)

                                                                                                                                                    aaTTGATCCCTT‑GTTCATAAATTAGCGGGGGGCTAATGGCGGTGGATTTGATCACCGA‑ACCTTCGGGCCAAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCT‑CCCACCAGTC‑GAGGTTTTCTTTCAGCGtg                                                                                         <  2:1019000/239‑1 (MQ=255)

                                                                                                                                                     aTAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGGTTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGGGATAGTTGAGGTTAATTCGCCCGCCTTTTCTTGCTGCGGAGACATTAG‑AACTTCGTCAGCGTTGATTTTTTCCTGAT‑CTAGGCGGGGTTGGTTTAACCGCTTACGCCGTTCGGAGTGCT‑CCCACCCCAC‑GAGGGttttttttcgcgcgg                                                                                        >  2:320163/1‑231 (MQ=255)

                                                                                                                                                     aTAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGGGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGGGCT‑CCCCCCAGGC‑CGGGGTTTCTTTTAGCGtgc                                                                                        >  1:168503/1‑239 (MQ=255)

                                                                                                                                                     aTAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCCTCTGGCGCAATATTG‑CCCACCGGGGAGGTTGTAGTTGCGGCTAATCAGCGCGCTTTTTCTTGCCGGGGAGACATCAT‑CACGTCGTCTGCGTTGATATGTTCCATGT‑CGACCAGGATGTGCTTGAACCGCGCACGCCGGTCGGTGGGCT‑CACCCCAGAC‑GCGGTTTTCATTCAACGggc                                                                                        >  2:393020/1‑239 (MQ=255)

                                                                                                                                                            ccTT‑GGTCATAAATCATCGAGGGGTCAATGGCGGTGGAATGGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCT‑CCCACCAGTC‑GAGGTTTTCTTTCAGCGTGCTGCCGGt                                                                                 <  1:77344/239‑1 (MQ=255)

                                                                                                                                                            ccTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGa                                                                                                                             <  1:220229/197‑1 (MQ=255)

                                                                                                                                                             cTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGGTAGTTGAGGTTAATCCGCGCGCCTTTGCTTGCTGCGGAGACATGAT‑CACCTCGTCAGCGTTGATTTGTTCCTGAT‑CGGGCAGGGGTTGCTTGAACCGCTGACGCCGTTCgggg                                                                                                                            >  1:585960/1‑195 (MQ=255)

                                                                                                                                                             cTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGa                                                                                                                                                                    >  1:125287/1‑158 (MQ=255)

                                                                                                                                                             cTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGa                                                                                                                                                                    <  2:125287/158‑1 (MQ=255)

                                                                                                                                                              tt‑GCTCAACAATCATCGAGGGGTCAATGGCGGTGGATTTGATCCCCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCT‑CCCACCAGTC‑GAGGTTTTCTTTCAGCGTGCTGCCGGTaa                                                                               <  1:107681/239‑1 (MQ=255)

                                                                                                                                                              tt‑GCACATCAATCCGCGAGGGGTCAATGGCGGGGGATTTGAACACCGA‑ACCTTCTGGCGCAATTTTG‑CCCACCGGGAAGGTGAGAGTTGAGTTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTTTTCCTGAT‑CGAGCAGGAGTTGCTTGACCCGCTGACGCCGTTCGGAGTGCT‑CCCTCCAGGC‑GAGGTTTTCTTTCAGCGTGCTGCCGGTaa                                                                               <  2:832669/239‑1 (MQ=255)

                                                                                                                                                               t‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CCCTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGCGTTGCTTGAAACGCTGACGCCGTTCGGAGTGCT‑CCCACCAGTC‑GAGGTTTTCTTTCAGCGTGCTGCCGGtta                                                                               >  1:142863/1‑236 (MQ=255)

                                                                                                                                                                   ccatcaATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCT‑CCCACCAGTC‑GAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGt                                                                           <  1:877232/238‑1 (MQ=255)

                                                                                                                                                                      tcaatcaTCGAGGGGTCAATGGCGGTGGGTTTTGTCACCCC‑ACCTTCTTGCGCAATATTA‑CCCACCGGGGAGGTGATCATTGGGGTTTATCCGCGCCGTTTTGCTTACCGCGGAGACAAGAT‑CAATTCTCCACACTTGATTTTTTTCTGATGCGGCCGGCAGTT‑CTTGAACCGCCGACCCCGTTTCCGGTGGT‑CCCACCAGCT‑GCGGTTTTTTATCaaccggccaccctgacacgccca                                                                        >  2:960236/1‑217 (MQ=255)

                                                                                                                                                                        aaacatcGGGGGGGCATGGGGGGTGGGTTTGATCCCCGA‑ACCTTTTGGCGCAATATTT‑CCCACCGGGAAGGGGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCT‑CCCACCAGTC‑GAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATaa                                                                      <  2:1036003/236‑1 (MQ=255)

                                                                                                                                                                           catgGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCT‑CCCACCAGTC‑GAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGt                                                                   <  1:951719/239‑1 (MQ=255)

                                                                                                                                                                           catcGAGGGGTCAATGGCGGTGGATTTGATCCCCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCT‑CCCACCAGTC‑GAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGt                                                                   <  1:165938/239‑1 (MQ=255)

                                                                                                                                                                               gAGGGGTCAATGGCGGTGGGTTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGGGTTGCTTGCACCGCTTACGCCGTTCGGGGTGTT‑CCCACCAGTG‑CGGGGTTTCTTTCAACCGGCGGCCGGTGACCCTccaacactctct                                                               >  2:720445/1‑228 (MQ=255)

                                                                                                                                                                                  gggTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCT‑CCCACCAGTC‑GAGGTTTTCTTTCAGCg                                                                                           <  1:761275/208‑1 (MQ=255)

                                                                                                                                                                                  gggTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCT‑CCCACCAGTC‑GAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCAt                                                             >  1:1045216/1‑238 (MQ=255)

                                                                                                                                                                                    ttCAATGGCGGTGGCTTTGATCACCGA‑ACTTTCTGGCGCAATAGTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGTTTTGTCTGGCGGCGGAGACATGAA‑CGCTTCTTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTACTTGAACCGCTg                                                                                                                                         <  2:1020859/161‑1 (MQ=255)

                                                                                                                                                                                    gTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGGGTGCT‑CCCACAAGTC‑GAGGGTTTCTTTCAGCGTGCTGCCGGGAACCTTCATAACGTCTTCATGca                                                          >  1:466141/1‑239 (MQ=255)

                                                                                                                                                                                    gTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCT‑CCCACCAGTC‑GAGGTTTTCTTTCAGCGGGCTGCCGGTAACCGGCCTAACGCCTTCATGca                                                          >  1:44587/1‑239 (MQ=255)

                                                                                                                                                                                      cAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCT‑CCCACCAGTC‑GAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGcaa                                                         <  1:720445/238‑1 (MQ=255)

                                                                                                                                                                                      cAATGGCGGTGGATTTGAATACCGG‑ACCTTCGGGCGCAATATTG‑GCCAACGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCT‑CCCACCAGTC‑GAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGcaa                                                         <  1:633597/238‑1 (MQ=255)

                                                                                                                                                                                      cAAGGGGGGTGGTTTTGTACCCCGA‑ACCTTGTGGCGAAATTTTC‑CCCCCCGGGAAGGTGATAGTTGAGGTTAATCCGGGCGTTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCT‑CCCACCAGTC‑GAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGcaa                                                         <  2:1045216/238‑1 (MQ=255)

                                                                                                                                                                                       aaTGGCGGTGGGTTTGAGCACCGA‑ACCTTCCGGCGCAATATTG‑CCCACCGGGAGGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGGTGGGGAGACATGAG‑CCCTTCCTCAGCGTTGGTTTGTTCCTGAT‑GTGGGCGGGGTTGTTTGTACCGCTGCAGCCGTTC‑GAGCGGTCCCCACCAGTCGGGGGTTGTTTTACAGCG‑GCGCCCGGCAACCGCCCTACCGTCTTAATTcaca                                                        >  2:151426/1‑236 (MQ=255)

                                                                                                                                                                                          ggCGGTGTTTTTTATCACCTA‑ACCTTCGGGCGCATTATTG‑CCCCCCGGGAAGGTGATAGTTGCGGTTAATCCGCGCGCTTTTGCTTGCGGCGGAGACATGAT‑CCCTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGACCCGCTGACGCCGTTCGGAGTGCT‑CCCACCAGTC‑GAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATc                                                    <  2:168503/239‑1 (MQ=255)

                                                                                                                                                                                              gTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGGGCT‑CCCACCAGTC‑GAGGTTTTCTTTCAGCGTGCGGCCGGTAACCGTCATAACGCGTTCATGCAACAATCCAAg                                                >  1:1025592/1‑239 (MQ=255)

                                                                                                                                                                                              gTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCCGGAAGGTGATAGTTGAGGTTAATCCGCGCGGTTTTCATTGCTGCGGGGACATTAT‑CCCTTCGTCAGCGTTGTTTTGTTCCTGGT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTcc                                                                                                                               >  1:1020859/1‑161 (MQ=255)

                                                                                                                                                                                                     tttttcccccacccTTCTGGGGCAATATTG‑CCCCCCGGGAAGGTGATAGGTGAGGTTAATCCGCGCGTTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCT‑CCCACCAGTC‑GAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTgcg                                          <  2:575041/227‑1 (MQ=255)

                                                                                                                                                                                                         gcccccgttcctttgcgtcttgtttG‑CCCGCCGGGAGGGTGTTCTGTGAGGTTTTCCCGCGCGTTTTTGCTTACTGCGGAGACATGAT‑TCCTCCGCACGGGATGATTTGTGCCTTTT‑CGAGCAGGATTGGCCTGGACCGCTGTCGCCGTGCGGAGTGCT‑CCTACCCGTC‑GAGGTTTTCTTTCATCGTGCTGCGTGTAACCGTCATAACGTCTTCATGCAACAATCCGATGCTGCTCAGa                                      <  2:88631/216‑1 (MQ=255)

                                                                                                                                                                                                         aagggccggttttgggggcAATGTTG‑CCCCCCGGGAAGGTGTGTGTGGAGGGTAATCCCCGCTCTTGTGCGTGCTGAGGGTGAAGTAG‑CCCTGCGTCCGCGTTGATTTGTTCCTGAG‑CGAGCAGGAGGTGCTTGAACCGCTGACGCCGTTCGGAGTGCT‑CCCGCCAGTC‑GAGGTTTTCTTGCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCGGCGCAGa                                      <  2:23305/222‑1 (MQ=255)

                                                                                                                                                                                                           gccgaagctttcgggagaatttgg‑CCCACCGGGAAGGGTTGAATGGAGGGTAAGCAGCGCGGGTGTGCTTGCTGCGGGAACGTGAT‑AACTTGTGCAGCGTTGATTTGTTCCTGCT‑CGGGCAGGAGTTGCTTGAACCGCTGACGCCGTGCGGAGTGCT‑CCCACCAGGC‑GAGGTTTTCTATCAGCGGGCTGCCGGTAACCGTCATAACGTCTTCAGGCAACACTCCGAGGCTGCGCAGATg                                    <  2:1016384/216‑1 (MQ=255)

                                                                                                                                                                                                           ccGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTTATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCT‑CCCACCAGTC‑GAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGca                                                          <  2:459147/216‑1 (MQ=255)

                                                                                                                                                                                                           ccGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCT‑CCCACCAGTC‑GAGGCTTTCTTTCAGCGTGCGGCCGGTAACCGTCCTACCGTCTTCATGTCACAATCCGAgcgggcccgaacgc                                   >  2:64660/1‑226 (MQ=255)

                                                                                                                                                                                                           ccGA‑ACCTTCGGGCGCAAGATTG‑CCACCCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCT‑CCCACCAGTC‑GAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGc                                   <  1:910231/239‑1 (MQ=255)

                                                                                                                                                                                                            cGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGATTGCT‑CCCACCAGTC‑GAGGTTTTCTTTCAGCGTGCGGCCGGAAACCGTCATAACGTCTTCATCCAAAAATCCGAGGCTGCGAGGATgaa                                  >  2:632037/1‑237 (MQ=255)

                                                                                                                                                                                                              a‑aCCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCT‑CCCACCAGTC‑GAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCACCAATCCGAGGCTGCGCGGATGCacc                                >  1:484152/1‑237 (MQ=255)

                                                                                                                                                                                                                aCCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCT‑CCCACCAGTC‑GAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACa                               <  1:32858/239‑1 (MQ=255)

                                                                                                                                                                                                                  cTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCT‑CCCACCAGTC‑GAGGTTTTCTTTCAGCGTGCTGCCGCTAACCGTCATAACGTCTTCATGCACCCATCCGAGGCTCAGCAGTTGCAACAt                              >  1:980986/1‑238 (MQ=255)

                                                                                                                                                                                                                    tCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCT‑CCCACCAGTC‑GAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATg                             >  1:718105/1‑237 (MQ=255)

                                                                                                                                                                                                                             atatTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCT‑CCCACCAGTC‑GAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGTTGCGCAGATGCAACATGAcctgcctctt                  >  1:23305/1‑230 (MQ=255)

                                                                                                                                                                                                                               tttG‑CCCGCCGGGAAGGTGTAAGTTGGGGTTAACGCTCGCGCTTTTTGTTGCTGCGGACACATGAT‑CTCCACGGCGGGGTTGATTTGTTCCGTGT‑CGGTCAGGGGTTGCTTGAACCGGTGACGCCGTCCGGAGTGCG‑CCCACCAGTC‑GAGGTTTTCTTTAAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCacac                <  2:200277/238‑1 (MQ=255)

                                                                                                                                                                                                                                       aCCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCT‑CCCACCAGCC‑GAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCAAAAGGTCTTCATGCCACAAACCCAGGCTGGCCGAAGGAAAAAAGACTTCCGGCAaaccacagg          >  2:446299/1‑235 (MQ=255)

                                                                                                                                                                                                                                         cGGGACGGTGATAGTTGAGGCTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCT‑CCCACCAGTC‑GAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGATGCGCCGATGCAACACGACTTCAGGcaaaaccacgggca       >  2:350715/1‑227 (MQ=255)

                                                                                                                                                                                                                                         cGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCT‑CCCACCAGTC‑GAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGCCTGCGAAGATGCAACATGACTTCCGCCACCCCACCTGCCa       >  1:163555/1‑239 (MQ=255)

                                                                                                                                                                                                                                         cGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCT‑CCCACCAGTC‑GAGGTTTTCTTTCAGCGGGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGGTGCCACATAACTTCCGGCACaccaccctcca       >  2:524987/1‑234 (MQ=255)

                                                                                                                                                                                                                                              aGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCT‑CCCACAAGTC‑AAGGTTTTCTTTCTGCGTGCTCCCGGTAAACGTCATAACGGCTTCCTGCAACAATCCGAGGCTGCGCAGATCCAAATAGACTCCCGGCACAACACCTGCCAAAAAg  >  2:967189/1‑239 (MQ=255)

GGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAATGACCAGAATAT‑CGCCCGCCTTGATCTTGTCATGT‑TTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTT‑GCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGA‑ACCTTCTGGCGCAATATTG‑CCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGAT‑CACTTCGTCAGCGTTGATTTGTTCCTGAT‑CGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCT‑CCCACCAGTC‑GAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAG  >  NC_000913/4522548‑4523019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`