| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 37,255 | A→C | 22.4% | G190G (GGT→GGG) | caiC ← | putative crotonobetaine/carnitine‑CoA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 37,255 | 0 | A | C | 22.4% | 117.5 / 5.3 | 74 | G190G (GGT→GGG) | caiC | putative crotonobetaine/carnitine‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base A (27/25); new base C (2/17); total (29/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.19e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATTCGGTAACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTG‑GCCCCGGCAGAAAACGCCGCCATCGCCGCA‑GTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGA‑AGTGCCA‑CATCTGTCA‑GGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCA > NC_000913/37022‑37477 | cATTCGGTAACGGTGGCGCGGTACTTCTGGACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTG‑GCCCCGGCAGAAAACGCCGCCATCGCCGCA‑GTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCttt < 1:930604/239‑1 (MQ=255)cATTCGGTAACGGGCGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCCCGACCAGCACAAAGGTG‑GCCCCGGCAGAAAACGCCGCCATCGCCGCA‑GTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCttt < 1:494336/239‑1 (MQ=255) gTAACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTG‑GCCCCGGCAGAAAACGCCGCCATCGCCGCA‑GTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCCCACTGCCAGGCGGAGGAATAT‑CCAGCGAAGCACAGGCTGTAATGGGTAATACCCACA‑CCTTtcggtcg > 1:169933/1‑239 (MQ=255) gcgcGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTG‑GCCCCGGCAGAAAACGCCGCCATCGCCGCA‑GTACACTGGCCATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACCATACCAGGCGGAGGAATAA‑CAAGCCAAGGGCAGGTTGTACTGGGTAAGCACCACA‑CCTTTCGGTAGGGGggcggg > 2:27775/1‑238 (MQ=255) tACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTG‑GCCCCGGCAGAAAACGCCGCCATCGCCGCA‑GTACACTGGCAATCGATATGAAAAGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGTAGGTGGTGCCGGa > 1:424182/1‑239 (MQ=255) tCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTG‑GCCCCGGCAGAAAACGCCGCCATCGCCGCA‑GTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGCCGTCATCGTCACGCAGTGCACACGGCCAGGCGGAGTAATAT‑CCAACAAAGCGGAGGGTGTAATGGGGAATCACCACC‑CCCTTCCGTCGGGGGGGGGGGGCGgggggg > 2:769431/1‑237 (MQ=255) gTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTG‑GCCCCGGCAGAAAACGCCGCCATCGCCGCA‑GTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCCTCGTCCCGCAGTGCACACTGCCAGGCGGAGTAATAT‑CCCGCGAAGCGCAGGTTGTAATGGGTAATCCCCACC‑CCTTTCGGTCGGGGGGGGGGGGCGGGGGGGaaagaaagt > 2:608679/1‑232 (MQ=255) gTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTG‑GCCCCGGCAGAAAACGCCGCCATCGCCGCA‑GTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGCAGACGTCATCGTCCCGCAGTGCACACTGCCAGGCGGGGTAATAT‑CCAGCGAAGCCCAGGTTTCAATGGGTAATCACCACA‑CCTTTCGGTCGGGGGGTGGGGCCGGGGGTGAAGGGAAtt > 1:518271/1‑239 (MQ=255) tCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTG‑GCCCCGGCAGAAAACGCCGCCATCGCCGCA‑GTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATAT‑CCGGCGAAGCGCAGGTTGTAATGGGTAATCACCAAA‑CCTTTCGTTCGGGAGGTGGTGCCGGGGGTGAAGGGaagt > 1:625925/1‑236 (MQ=255) gcgcGGGCGCTGTATTTCTCGACCAGCACAAAGGTG‑GCCCCGGCAGAAAACGCCGCCATCGCCGCA‑GTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGAGCACACTGCCAGGCGGGGTAATAT‑CAAGCGAAGCGCAGGTTGTAATGGGGAATCACCCCA‑CCTTTCGTTCGGGAGGGGGTGCCGGGGGGGAAGAGAATTTCCGCCGTATc > 2:304429/1‑239 (MQ=255) tttttctctcagcccaccaaaaGGTG‑GCCCGTGAAAAAACCCCCGCCATCCACCAA‑GTACCCGGGAAAACGATATGAAACGCAGGCATGACCGGCGGGTAGACGTGTGCTTCCAGGAGTGCACTCGTCCGGGCGGAGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCCCCCCC‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGAt < 2:266498/220‑1 (MQ=255) gtttCTTCACCCGCACCAAGGGGTGCCCAGGCACAAAACGCCGCCACGCCCACA‑GTACCCGGTCAATCGATATAAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACCCTGCCGGGTGGAGTAATAT‑CCAGCGACGCGCAGGTTGTAATGGGTAATCACGCCC‑CCTTTCGGTCGGGAGGTGTTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAg < 2:616530/238‑1 (MQ=255) aaagaaacgggCAAAAGGGGG‑GCCCGGGAAGAAAACCCCGCCATCGCCGGA‑GTACCCTGGCAAACGATAGGAAACGCCGGACTTCCCGTCAGGTAGCCGTAATCGTCACGCAGTGCACCCTGCCAGGCGGAGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAgcgg < 2:839989/229‑1 (MQ=255) cgcgcccAGAACAAGGGTG‑GCCCCGGCAGAAAACGCCGCAATCGCCGCA‑GTACGCGGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAgcggcg < 1:1031168/234‑1 (MQ=255) gACCAGCACAAAGGTG‑GCCCCGGCAGAAAACGCCGCCATCGCCGCA‑GTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGGGCACACTGCCAGGCGGAGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGGGGGGGTGCCGGCGGTGAAGAGAATTTCCGCCGTATctgcagcgggatgcggcgggg > 2:136348/1‑219 (MQ=255) cccacccacaaGGTG‑GCCCCGGCACAAACCCGCGCTATCGCCCCA‑GTACACGGGCAATCGATATGAAACGCAGGCATTGCCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACC‑CCTTTCGTTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGc < 2:1059517/230‑1 (MQ=255) ccAGCACAAAGGGG‑GCCCCGGCAAAAAACGCCGCCATCGCCGCA‑GTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCa < 1:387450/239‑1 (MQ=255) ccAGCAAAAAGGTG‑GCCCCGGCAGAAAACCCCGCCATCGCCGCA‑GTACCCTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCa < 1:955911/239‑1 (MQ=255) aacagcaAAGGTG‑GCCCCGGCAAAAAACGCCGCCATCGCCGCA‑GTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCa < 1:627381/233‑1 (MQ=255) cacaAAGGTG‑GCCCCGGCAGAAAACGCCGCCATCGCCGCA‑GTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCCCACTGCCAGGCGGAGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGGAATCACCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGGGGGGAAGAGAATTTCCGCCGTATCGTAAGTCGATAGcggcgggggaaaga > 2:111712/1‑231 (MQ=255) aaaaGGTG‑GCCCCGGCAAAAAACCCCGTCATCGCCGCATGAACA‑TGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATAT‑CCAGCGGAGCGCAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGcac < 1:303888/238‑1 (MQ=255) aaaGGTG‑GCCCCGGCAGAAAACGCCGCCATCGCCGCA‑GTACCCTGGCAATCGATATGAAACTCAGGCATTGCCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGcaca < 1:114309/239‑1 (MQ=255) gCCCCGGCAGAAAACGCCGCCATCGCCGCA‑GTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGcggcgggggctagcctggtccttt > 1:839989/1‑221 (MQ=255) gCCCCGGCAGAAAACGCCGCCATCGCCGCA‑GTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGAGGTGGGGCCGGAGGTGAGGAGAATTTCCCCCGTCTCGGCGGTCGATACCGGCGGTggcatggacaaaggggg > 2:172424/1‑223 (MQ=255) gCCCCGGCAGAAAACGCCGCCATCGCCGCA‑GTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATAT‑CCAGCGAAGCCCAGGTTGTAATGGGTAATCACCAAA‑CCTTTCGGTCGGGAGGGGGGGCCGGGGGTGAGGAGAATTTCGCCCGTATCGTCAGGCGATACaggcgggggagagaaaaagagggg > 2:923436/1‑221 (MQ=255) gccACCGCCGCA‑GTACACTGGCAATCGATAGGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAg < 1:179346/239‑1 (MQ=255) acaggggtccttGGCAAGTGAAATGAACCGAAGGCATTACCGTCAGGTAGACGTCCTCGTCCCGCAGTGCACACTGCAGGGCGGAGTAATAT‑CCAGCGAAGCGCAGGTGGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATCTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAg < 2:399166/226‑1 (MQ=255) ccatGGCAATGGTTTGGACACGCAGGCCTTGCCGTCAGGTAAACTACGTCGTCACGGGGTGCCCCCCGCCAGGCGGAAT‑ATATGCCAGCGAAGCGGAGGTTGTAATGGGTAATCACCCCA‑CCTTTCGGTCGGGAGGTGGTGCGGGAGGTGAAGAGATTTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACt < 2:350889/236‑1 (MQ=255) acTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGAGGGGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGGGCATATAACCAGAGGGACGGGTGTTGAttttttacgtagggaaaagaacac > 2:921033/1‑220 (MQ=255) aTCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGGATAGCCCAAGGTGGAGGGGTGTTGGTTTTTCCATTTAGTAAAAGAACt > 1:399166/1‑231 (MQ=255) aTCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCAAA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGGTAGCGGCGGTGGATAGGACAAGGTGGCCGGGTGTTGATTTTTCAGTTGGGTAAACGACATCACCCca > 1:350889/1‑238 (MQ=255) cggtaGGTAGACGTCAGCTGCACGCAGTGCCCGCGTCCAGGCGGAGTAATCT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGA‑AGTGCCA‑CATCTg < 1:27775/235‑1 (MQ=255) ccggTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGCCAGTCGATAGCGCCGGTGCATAGCACAAAGCAGAGGGTTGTTGGTTTTTTCTTTTAGTCAACACAACCACGCCAGCATCAACTGGAGAGTG‑CA‑CATATGTCa > 2:438748/3‑239 (MQ=255) gACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGGCCACGGTGGCAGGTTGTTGATTTTTCAGTTTGGTAAACGAACTCCGGCCCCCATCCGCGGGA‑AGTGCCC‑CATCGTTCA‑GGCaaa > 1:16213/1‑239 (MQ=255) tCATCGTCACGCAGTGCAAACTGCCAGGCGGAGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACG‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACg < 1:1046163/190‑1 (MQ=255) cATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATAA‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGA‑AGTGCCA‑CATCTGTCA‑GGCAAATGTGc < 1:136348/239‑1 (MQ=255) aTCGTCACGCAGTGCACACTGCCAGGCGGAGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGGACAAGGTGGCAGGTTGTTGGTTTTTCAGTTGAGTTAACGAACTAAAGCCCTCATACACGGGA‑AGTGCCC‑CCACTTTCA‑GGGACATTTGc > 1:1051007/1‑238 (MQ=255) tCGGCAGGCGGTGCCCCCTGCCGGGGGGGTTAATAT‑CCGGGGAGGGGCGGGTTGTAAGGGGTAATCCCCACC‑CCTTTCGGGGGGGGGGGGGTGCCGGGGGTGAAAGAAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGA‑AGTGCCA‑CATCTGTCA‑GGCAAATGTGCCg < 2:1051007/239‑1 (MQ=255) ccGTCAAGCAGTGCACCCTGCCAGGCGGAGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCA‑CACACCCTTTCGGTGGGGGGGTGGTGCCGGAGGTGAAAAAAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGA‑AGTGCCA‑CATCTGTCA‑GGCAAATGTGCCg < 1:261840/238‑1 (MQ=255) gCAGTGCACACTGCCAGGCGGAGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGA‑AGTGCCA‑CATCTGTCA‑GGCAAATGTGCCGCAATTGa < 1:556189/239‑1 (MQ=255) agggCACACGGCCAGGCGGAGTAATAG‑CCAGCGAAGCGCAGGTTGTAAGGGGTAATCACCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGA‑AGTGCCA‑CATCTGTCA‑GGCAAATGTGCCGCAATTGAg < 1:172424/235‑1 (MQ=255) tGCACACTGCCAGGCGGAGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGGGGATAGGACAAAGGCGCAGGTTGTGGATTTTTTAGTTGAGTAAAAGAACACCCACCCACATCACAGGGA‑AGTGCCA‑CACCTGTCACCGCAAAtggacgccaatgcaagg > 2:66362/1‑224 (MQ=255) cccacggacAGGCGGAGTAGTGC‑CCAGGGAAGCGAAGGTTGTAATGGGTAATCCCCACC‑CCTTTCGGTCGGGAGGTGGGGCCGGGGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGAGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGA‑AGTGCCA‑CATCTGTCA‑GGCAAATGTGCCGCAATTGAGTgg < 1:662662/229‑1 (MQ=255) cacaCTGCCAGGCGGAGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGGGGCCGGTTGTTGATTTTTCCGTTTAGTAAACGAAATCACGCCATCATCAGCGGGA‑AAGTCCA‑CATCTTGCA‑GGCAAATTTGCCGCAATTTAGGGGc > 1:539661/1‑238 (MQ=255) ccacTGCCAGGCGGAGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCCCC‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTGCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGA‑AGTGCCA‑CATCTGTCA‑GGCAAATGTGCCGCAATTGAGTGGCa < 1:111712/237‑1 (MQ=255) acTGCCAGGCGGAGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGGACTCACGCCATCATCAGCGGGA‑AGGGCCC‑CATCTGTCA‑GGCAAATGTGCCGCAATTGAGTGGCACCt > 1:891239/1‑239 (MQ=255) gCCAGGCGGAGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCCTAGCACAAGGTGGCCGGTTGTTGATTTTTCAGTTGAGTAAACGAACCCACCCCCTCATCAACGGGA‑AGTGCCA‑CAACTGTCA‑GGCAAATGGTCCGCAATTTAGTGGGAtttt > 1:989892/1‑237 (MQ=255) cAGGCGGAGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGGGGCAGGTTGTTGGTTTTTCAGTTGAGTAAACGAACTCACGCCATCATCCGCGGGA‑AGGGCCC‑CATCTGTCA‑GGCAAAATGGCCGCAATTGAGTGGCATCTTCct > 1:530944/1‑238 (MQ=255) cggCGGGGTTATTC‑CCAGGGGAGCCGAGTTTTTAAGGGGAAAACCCCACC‑CCTTTCGGGCGGGGGGTGGTGCCGGGGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCCCAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGA‑AGTGCCA‑CATCTGTCA‑GGCAAATGTGCCGCAATTGAGTGGCATCTTCctgc < 2:625925/238‑1 (MQ=255) ggAGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCCCCACC‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGA‑AGTGCCA‑CATCTGTCA‑GGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGa < 1:380586/238‑1 (MQ=255) aGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCAACGAACAAGGGGGGAGGTTTTTGATTTTTTAGTTGGGGTAACGGACTCACGCCCACATCAACGGGA‑AGTGGCA‑CATATGTGA‑GGCCAATATGACCCAATTTAGTgggcgcttccactgcaaca > 2:165501/1‑222 (MQ=255) aGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGGGCATGACACAAAGTGGCAGGGTTTTGTTTTTTTAGTTTGGGAAACGCAAACACCCCCACCACATCGGGG‑AATGCCACCATCTGTCA‑‑GCAAATATGCCCCCATTAAgggcgagtctccctgtggatc > 2:1026795/1‑219 (MQ=255) ttttttccatgggAAGCGCGGTTTGTTATGGGTAATCCCCTCC‑CCTTGGGGTCGGGAGGGGGGTCCGGGGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGA‑AGTGCCA‑CATCTGTCA‑GGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATc < 2:539661/226‑1 (MQ=255) aaTGT‑CCAGGGAAGCGCAGGTTGTAATGGGTAATCCCCTCC‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGA‑AGTGCCA‑CATCTGTCA‑GGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTg < 1:822647/238‑1 (MQ=255) aaTAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGA‑AGTGCCA‑CATCTGTCA‑GGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTg < 1:893829/238‑1 (MQ=255) aattgcgggCGAAGGGCAGGTTTTAATGGTTAATCCCCCCC‑CCGTTGGGTCGGGGGGGTGGGCGGGAGGTGAAGAGAATTTCCGCCGTATCGGCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGA‑AGTGCCA‑CATCTGTCA‑GGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTg < 2:518271/230‑1 (MQ=255) tat‑CCAGGAAAGCGCAGGTTTAAATGGGTAATCCCCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGA‑AGTGCCA‑CATCTGTCA‑GGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTg < 1:1094892/239‑1 (MQ=255) tgcccacggaaggccAGGTTGTAAGGGGTAACCCACACT‑CCTTTCGGTCGGGAGGTTGTGCCGGAGGTGAAGAAAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCGCGCCATCATCAGCGGGA‑AGTGCCA‑CATCTGTCA‑GGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTg < 1:902526/225‑1 (MQ=255) gccggtgggggtttagaagagTAAGGGTTACTCCCCACC‑CCTTTCGGGGGGGGGGGGGTGGCGGAGGGGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGA‑AGTGCCA‑CATCTGTCA‑GGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTg < 2:181813/219‑1 (MQ=255) gacaagcggcgtgTTGTAATGGGTAAGCCCCCAG‑CCTTGCGGGGGGGAGGTGGTGCGGGAGGTGAAGTGAATTTCCGCCGTTTCGTCAGTCGGTAGCGGCGGAGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGA‑AGTGCCA‑CATCTGTCA‑GGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACa < 2:118473/227‑1 (MQ=255) aGCGAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTAt < 1:35411/83‑1 (MQ=255) aGCGAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTAt > 2:35411/1‑83 (MQ=255) cGAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGAACAAGGGGGCAGGTTGTTGTTTTTTCAGTTGGGTAAAAGAAATCACGCCACCATCAGCGGGG‑AATGCCC‑CATCTTTAC‑GGCAAATGTGCCGCAATTTAGTGGGCACTTCCTGCTGAAATTGTTTATACATa > 1:370105/1‑239 (MQ=255) gaatgtgaGGTTGTAATGGGTAATCCCCACA‑CCTTTCGGTCGGGAGGGGGTGCCGGAGGTTAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGA‑AGTGCCA‑CATCTGTCA‑GGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACa < 1:864227/229‑1 (MQ=255) gAAGGGCGGGTTGTTATGGTTAAGCCCCACC‑CCTTTCGTTCGGGAGGGGTGGCCGGGGGTGAAGAGAATTTCCGCCGTTTCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGA‑AGTGCCA‑CATCTGTCA‑GGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACa < 2:424182/236‑1 (MQ=255) gAAGCGTGGGTTGTAATGGGTAATCCCCACC‑CCTTGCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGA‑AGTGCCA‑CATCTGTCA‑GGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATa < 1:769431/238‑1 (MQ=255) gAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGA‑AGTGCCA‑CATCTGTCA‑GGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACa < 1:304429/236‑1 (MQ=255) aaaGCGTAGGTTGTAATGGGTAATCACCAAC‑CCGTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGA‑AGTGCCA‑CATCTGTCA‑GGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACa < 1:954565/235‑1 (MQ=255) gcggAGGTTGGAAGGGGTAATCACCGCA‑CCTTTGGGTCGGGGGGTGGTGCCGGAGGGGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGA‑AGTGCCA‑CATCTGTCA‑GGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGa < 1:119182/238‑1 (MQ=255) tcagtgtggaaggggTAATCCCCACC‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTTAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGA‑AGTGCCA‑CATCTGTCA‑GGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATa < 1:401866/226‑1 (MQ=255) cAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCAAAGAACAAAGGGGCAGGTTGTTGATTTTTTAGTTGAGTAAAAGAAACCCCGCCAGCATCCACCGGG‑AGTTCCC‑CCTCCGTCA‑GGCCAATGAGCCCCCATTTGTGGGCCGCCTTCTGCTGAAACTGGTAGTAAATAGGAAAGa > 2:631234/1‑239 (MQ=255) aaTGGGTAATCCCCACA‑CCTTGCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGA‑AGTGCCA‑CATCTGTCA‑GGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCa < 1:246419/239‑1 (MQ=255) aaTGGGTAAGCCCCACC‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGA‑AGTGCCA‑CATCTGTCA‑GGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCa < 1:173809/239‑1 (MQ=255) caccacA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGA‑AGTGCCA‑CATCTGTCA‑GGCAAATGTGCCGCAATTGAg < 1:42088/182‑1 (MQ=255) | CATTCGGTAACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTG‑GCCCCGGCAGAAAACGCCGCCATCGCCGCA‑GTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATAT‑CCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACA‑CCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGA‑AGTGCCA‑CATCTGTCA‑GGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCA > NC_000913/37022‑37477 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |