Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 762,113 T→C 17.5% S198P (TCC→CCC)  sucB → dihydrolipoyltranssuccinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913762,1130TC17.5% 175.1 / 3.7 76S198P (TCC→CCC) sucBdihydrolipoyltranssuccinase
Reads supporting (aligned to +/- strand):  ref base T (41/18);  new base C (4/12);  total (45/31)
Fisher's exact test for biased strand distribution p-value = 3.01e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCTGGCTGAACACAATCTCGACGCCAGCGCCATTAAAGGCACCGGTGTGGGTGGTCGTCTGACTCGTGAAGATGTGGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTATCGACGGCGATGACGTGGTTTACCACAACTATTTCGACGTCAGCATGGCGGTTTCTACGCCGCG  >  NC_000913/761883‑762351
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                              
ggttggcgaaacaacatccggACGGCAGCGCAGTTAAAGGCACCGGTGTGGGTGGTCGGCTGCCGCGTGAAGGTTGGGAAAAACTTATGGCGAAACCCCGGGCGAAAGGGGCTTGTCCGGCAGCGGCTGCTCCGGCGGCCCAACCGGCTTGGGCGGCACGTAGGGAAAAACGTTCCCAGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCAAAAACCTCCACCGcc                                                                                                                                                                                                                                        <  2:603883/219‑1 (MQ=255)
           cacaATCTCGACGCCAGCGCCATTAAAGGCACCGGTGTGGGTGGTCGTCTGACTCGTGAAGATGTGGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACCCGCCTGCGTAACCGGGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCAc                                                                                                                                                                                                                             >  1:896853/1‑239 (MQ=255)
            ccacctcgcgccgccgccccATAAAAGGCCCGGGGGGGGGGGGCCGTCTGACTGGGGAGGGTGGGGAAAACCTCCTGGCAAAGCCCCGGGCGAAAGGGCCCGGTCCGGCGGGGGCTGCCCCGGCGGCGCACCCGGCTCGGGCTGCCGGTAGTGAAAACCGTGCCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACCCCACCGCCATGCTGACCACg                                                                                                                                                                                                                            <  2:213938/221‑1 (MQ=255)
                   cggccccAGGGCCATTAAAGGCACCGGTGGGGGTGGTCGTCTGACTCGTGAAGATGTGGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCCCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACg                                                                                                                                                                                                                     <  1:92298/234‑1 (MQ=255)
                     aCGCCAGCGCCATTAAAGGCACCGGTGTGGGTGGTCGTCTGACTCGTGAAGATGTGGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACCaa                                                                                                                                                                                                                   >  1:786301/1‑239 (MQ=255)
                          aGCGCCATTAAAGGCACCGGTGTGGGTGGTCGTCTGACTCGTGAAGATGTGGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACCCCACCGCCATGCTGACCACGTTCAACGAAGTCaa                                                                                                                                                                                                              >  1:1038750/1‑239 (MQ=255)
                          aGCGCCATTAAAGGCACCGGTGTGGGTGGTCGTCTGACTCGTGAAGATGTGGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGGGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAAACCCACCGCCATACTGACCACGTTCAACAAAATCaa                                                                                                                                                                                                              >  2:87306/1‑239 (MQ=255)
                            cgcCATTAAAGGCACCGGTGTGGGTGGTCGTCTGACTCGTGAAGATGTGGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACa                                                                                                                                                                                                            >  1:707174/1‑239 (MQ=255)
                                 ttAAAGGCCCGGGTGTGGGTGGTCGTCTGACTGGTAAAGATGTGGAAAAACATCGGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAg                                                                                                                                                                                                       <  1:363416/239‑1 (MQ=255)
                                 ttAAAGGCCCCGGTGGGGGGGGTTGTGTGCCTCGGGAAGGTTGGGAAAAACGTCGGGCGAACGCCCGGGGAAAAGGGCCTGCTCCGGCAAGGGGTGCTCCGGGGGCGCAACCGGCTTTGGGTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAg                                                                                                                                                                                                       <  2:896853/239‑1 (MQ=255)
                                 ataaggctcccGGTGGGGGGGTCGGCTAGACGCGGGAGGTTGGGAAAAACCACCTGGCAAAGCCCCCGCTGAAGGGGTCGCTCCGGGAGGCGGCGGCCCCGGCGGCGCACCCGGTGTGGGCGGCTGGTGGTAAAAACCGGTTCCCGATAACCCGCCTGCGTAAGCTGGTGGCAGAGCTTCTGCGGGAAGCGAAAAACCCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAg                                                                                                                                                                                                       <  2:205204/230‑1 (MQ=255)
                                   aaaGGCCCGGGGGTGGGTGGTCGTCGGACTCGTGAAGATGTGGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGcc                                                                                                                                                                                                     <  1:574293/239‑1 (MQ=255)
                                         aCCGGTGTGGGTGGTCGTCTGACTCGTGAAGATGTGGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCCGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGCTTAt                                                                                                                                                                                               >  1:39841/1‑239 (MQ=255)
                                         aCCGGTGTGGGTGGTCGTCTGACTCGTGAAGATGTGGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGAGTAt                                                                                                                                                                                               >  1:926921/1‑239 (MQ=255)
                                         aCCGGTGTGGGTGGTCGTCTGACTCGTGAAGATGTGGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGACGGTCTGCTGGAAGCGAAAAACTCCACCCCCATGCTGCCCACGTTCACCGAAGTAAAAATGAATCCGATTag                                                                                                                                                                                               >  1:942390/1‑238 (MQ=255)
                                           cGGTGTGGGTGGTCGTCTGACTCGTGAAGATGTGGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGGGTCCCGATGACTCCCCTCCGTAAGCGTGTGGCCGAGCGTCTGCTGGAAGCGAAAAACTCCCCCCCCCTGCTGACCACGTTCAACGAAGACAACATGAAACCGATTATga                                                                                                                                                                                             >  2:239628/1‑238 (MQ=255)
                                           cGGTGTGGGTGGTCGTCTGACTCGTGAAGATGTGGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGCCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGACCGTCTGCTGGAAGCGAAAAACTCCACCGCCATCCTGCCCACGTTCACCGAGGTCACACTGAAGCCGATTACgg                                                                                                                                                                                             >  1:573238/1‑239 (MQ=255)
                                             ggtgggggtcggcgtgcgatccGTGAAGATGGGGAAAATCCTGTGGCAAATGCCCGGGCGAAAGGGTCTGCGCGGGGCGGGCGTGCCCGGGCGGCGCACCGGGCTCGGGGTGCACTAAGGGAAAAACGTGTCCGGAGGCCCCGCCTGGGAAAGCGTGTGGCAGAGAGTCTGCTGGAAGCAAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGa                                                                                                                                                                                            <  2:547049/218‑1 (MQ=255)
                                                tgggtggTCGTCTGACTCGTGAAGATGTGGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAAATCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTg                                                                                                                                                                                        >  1:604534/1‑239 (MQ=255)
                                                     ggtCGTCTGACTCGTGAAGATGTGGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTaa                                                                                                                                                                                   >  1:63033/1‑239 (MQ=255)
                                                      gttgggtcgccgcGTGAAGTGGGGAAAACACGTGTGGCGAACGCCCCGGGAAAGGTGGCCGCCGCGGGAGCGGCTGCTCCGGCGGAGAAACCGGGTCGGGGTGCACTGAGTGAAAAGCGGGTCCCGATGACTCGCCTGGGTAAGCGGGTGGCAGGGCGTCTGCGGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTa                                                                                                                                                                                    <  2:631949/226‑1 (MQ=255)
                                                        cgtcTGACTCGTGAAGATGTGGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCa                                                                                                                                                                                >  1:48536/1‑239 (MQ=255)
                                                        cgtcTGACTCGTGAAGATGTGGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAAAGGGTCCCGATGACTCGCCCGCGTAAGCGTGTGGCCGAGCGTCTGCTGGAAGCGAAAAACCCCACCGCCCTCTCGGCCACGTTCAACCAAGCTACCATGAACACGATTATggctgtcggacacga                                                                                                                                                                                >  2:665635/1‑226 (MQ=255)
                                                         ccgtttgcccGTGAAAAGGGGGAAAAACCATTGGGCAAAAGCCCGGGGGAAAGAGTCTGCCCCGGCAGGGGCTTCCCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAg                                                                                                                                                                                  <  1:54035/229‑1 (MQ=255)
                                                          tcTGACTCGTGAAGATGTGGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGAAGCGGCTGATCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACCCCCCTGCGTAAGCGGGTGGCCGAGCGTCCGCTGGAAGCGAAAAACTCAACCGCCATGCTGACCACGTTCACCGAATACAACAGCAAGccagtacaggacaagcgaaaccaga                                                                                                                                                                              >  2:566695/1‑216 (MQ=255)
                                                                             ggAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGCACTGCGTAAGCAGAACGGCGAAGCGTTTGaaa                                                                                                                                                            >  1:867139/1‑239 (MQ=255)
                                                                                    aCATCTGGCGCAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCGGGTGGCAGCGAACAACCCCACCGCCATGCTGACCACGTTCAACGAAGTACACATGAAGCCCGTTTCGGGTGTTGGTAAGCATGACGGGGAAAGGTTTaaaaaagaac                                                                                                                                                      >  2:189349/1‑235 (MQ=255)
                                                                                      cATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCCCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGGTCAACGAAATCAACATAAAGCCGATTATGGAGCTCCGTAAACAATACGGGGAAGCGTTTGaaaaaacccg                                                                                                                                                     >  2:678209/1‑234 (MQ=255)
                                                                                                  gCCCCGGCGAAAGTGCTTGCTCCGGGTGCGGCTCCCCGGGGGGCCGCACCGGCTTGGGCGCGACGAGGGTAAAAACGTGCCGCGCGGACTCGCCTGGGTAAGCGTGGGGCAAGGCGTCTGTGGGAAGCGAAAAGCTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCt                                                                                                                                         <  2:766943/239‑1 (MQ=255)
                                                                                                     cgccggagagtgtgtcgcccccGGCAGCGGCGGCCCCGGCGGTGCACCCGGGTCGGGCTGCACGGAGTGAAAAGCGTGCCCCGCTGCCTCGCCGGCATGAGCGGGCGGCAGAGGGTCGGGTGGAAGAAAAAAACCCCCCCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTc                                                                                                                                          <  2:672111/217‑1 (MQ=255)
                                                                                                      cGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAg                                                                                                                                                                                                       >  1:365787/1‑173 (MQ=255)
                                                                                                      cGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGGGAAGCGTTTGAAAAACGCCACGGCATCCGCCTgg                                                                                                                                       >  1:1103084/1‑237 (MQ=255)
                                                                                                        ggCGAAAAGGTCTGCCCCGGGAGCGGCTGCTCCGGGGGCGCACCCGGCTTTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCt                                                                                                                                    <  1:397719/239‑1 (MQ=255)
                                                                                                                     tGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCtttt                                                                                                                        >  1:509440/1‑237 (MQ=255)
                                                                                                                     tGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCt                                                                                                                        >  1:40390/1‑239 (MQ=255)
                                                                                                                     tGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGGGAAGCGTTTGAAAAACGCCAAGGCATCCGTCTGGGCTTTATGTCCtttt                                                                                                                        >  1:893321/1‑237 (MQ=255)
                                                                                                                                  ggCTGCTCCGGCGGCCAACCCGGCCGTGGCTGACGGTAGTGAAAAACGTGCCCGGAGGACCCGCCGGCGAAAGCGGGTGGCAGAGCTTCTGCTGGAAGCAAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCg                                                                                                             <  1:665635/238‑1 (MQ=255)
                                                                                                                                   gctgctCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTTAAGCGTTTGAAAAACGCCACGGCATCCGTCAGGGCCTTATGTCCTTCTAAGTGaaacgggg                                                                                                           >  2:695568/1‑234 (MQ=255)
                                                                                                                                    ctgctCCGGCGGACCAACCGGCTCGGGCTGCACGTAGTGAAAACCGTGCCCCGATGACTGGCCTGCGAAAGCGGGTGGCAGGGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCggt                                                                                                           <  1:678209/238‑1 (MQ=255)
                                                                                                                                        tCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACCCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTCCGGAGCAGCGTTTGAAAAAGCCCAACGACGCAGTTCAGGCTTTTTGTCCTTCTCCGTGAAAACGGGGGTTg                                                                                                      >  2:50734/1‑239 (MQ=255)
                                                                                                                                                ccacaccggtttgggCTGCCGGTGGTGAAAAACGGGCCCCAATGCCTGCCTCGGGTAAGCGGGTGGCAGGGCGTGTGGTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCt                                                                                               <  2:48536/225‑1 (MQ=255)
                                                                                                                                                                 gCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGTTGGTTGAAGCCCTGAAACGTTACCCGGAAgg                                                                             >  2:836437/1‑238 (MQ=255)
                                                                                                                                                                   aCGTAGTGAAAAACGTGTCCGGATGACTCGCCTGCGTAGGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTg                                                                            <  1:905980/238‑1 (MQ=255)
                                                                                                                                                                   aCGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAACCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACCTGAAAGCGCTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGa                                                                           >  1:214767/1‑239 (MQ=255)
                                                                                                                                                                       tgggAAAAAACTGTGCCGGATGCCTCGCCGGGGGAAGCGTGTGGCAGAGCGTTGGCTGGAAGCGAAAACCCCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGaa                                                                          <  1:683425/234‑1 (MQ=255)
                                                                                                                                                                              aaCGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTCCGTAAGCAGTACGGTGaa                                                                                                                                                                      >  1:237739/1‑137 (MQ=255)
                                                                                                                                                                                   gtCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAACCGTTACCCGGAAGTGAACGCTTCTATCGCCg                                                            >  1:380871/1‑238 (MQ=255)
                                                                                                                                                                                   gtCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCtt                                                                     >  1:1025207/1‑229 (MQ=255)
                                                                                                                                                                                   gtCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTATCGACgg                                                           >  1:901668/1‑239 (MQ=255)
                                                                                                                                                                                   gtCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTATCGACg                                                            >  1:61359/1‑238 (MQ=255)
                                                                                                                                                                                   gtCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGGGAACGCTTCTATCGACgg                                                           >  1:718168/1‑239 (MQ=255)
                                                                                                                                                                                   gacccGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGCAAGCGAAAAACTCAACCGCCATGCTGACCACGTTCAACGAAGTAAACATGAAGACGATTATGGAGCTGCGTAAGCAGTAAGGTGCAGCGATTGAAAAACGCCGCGGCATCCGTCTGGGCTGTCTGTCAGTCTACTGTAACGCGTGGGTTGAAGCCCTCGAACGTGTCCCGGacggcacacgtgtactcgcggg                                                           >  2:897655/3‑218 (MQ=255)
                                                                                                                                                                                     cccGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACCCTTCTATCGCCGGCg                                                         >  1:947758/1‑239 (MQ=255)
                                                                                                                                                                                         aatgccctgCCTGGGTAACCGTTGGGGAGAGCGTTGGAGGGAACGGAAAACCGCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTATCGACGGCGATg                                                      <  1:318272/231‑1 (MQ=255)
                                                                                                                                                                                            gccTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTATCGACGGCGATGACg                                                   <  1:239628/237‑1 (MQ=255)
                                                                                                                                                                                                cGCCTGCGGAAGGGGGTGGCAGAGCGTCGGCTGGAATGCAAAAACCCCCCCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTATCGACGGCGATGACGTGGt                                               <  1:79395/239‑1 (MQ=255)
                                                                                                                                                                                                 gCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTATCGACGGCGATGACGTGGtt                                              >  1:785806/1‑239 (MQ=255)
                                                                                                                                                                                                 ccgtGCGTAGACGTGTGGCAGGGGTTATGGAGGAAGAAAAAAACCGCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTATCGACGGCGATGACGTGGtt                                              <  1:44551/236‑1 (MQ=255)
                                                                                                                                                                                                  ccTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAACCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGGGAACGCTTCTATCGACGGCGCTAACGGGGttt                                             >  1:441419/1‑239 (MQ=255)
                                                                                                                                                                                                       cGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTATCGACGGCCATGCAGTGGTTTAcc                                          >  1:401627/1‑238 (MQ=255)
                                                                                                                                                                                                            gCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTACCGCCGGCGCTGCCGGGGTTTACCACAAca                                    >  2:991138/1‑238 (MQ=255)
                                                                                                                                                                                                            gCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGGTGAAGCCCTGGAACGTTACCCGGAAGGGAAAGCTACTATCGACGGCGACGACGTgggtaccccaaaat                                    >  2:381166/1‑227 (MQ=255)
                                                                                                                                                                                                                gtgGCAGGGCGTATGCTGGAAGCAAAAAACCCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTATCGACGGCGATGACGTGGTTTACCACAACTAtt                                 <  1:151637/238‑1 (MQ=255)
                                                                                                                                                                                                                gtgGAAGGGCGTCTGCTGGAAGCGAAAACCCCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTATCGACGGCGATGACGTGGTTTACCACAACTAtt                                 <  1:87306/238‑1 (MQ=255)
                                                                                                                                                                                                                 tgGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCCCGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGGGACCGCTTCTACCGACCGCGAGACCGAGGTTTACCAAAAAAATTTc                               >  2:890236/1‑239 (MQ=255)
                                                                                                                                                                                                                 tgGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGAACTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAAACCTTCTCTCGAGCGCGTTGCGCTGGTTTACCACAAccaattt                               >  2:799135/1‑233 (MQ=255)
                                                                                                                                                                                                                 tgGAAGTGCGTCTGCTGGAAGCGAAAAACCCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTATCGACGGCGATGACGTGGTTTACCACAACTATTTc                               <  1:316911/239‑1 (MQ=255)
                                                                                                                                                                                                                  ggCAGGGCCTCTGCGGGAACGAAAAAACCCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTATCGACGGCGATGACGTGGTTTACCACAACTATTTc                               <  1:890236/238‑1 (MQ=255)
                                                                                                                                                                                                                      gggtgttggtgggaacgaaaaaaCCCCCCCGCCAGGGTGACCACGTTCAACGAAGGCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTATCGACGGCGATGACGTGGTTTACCACAACTATTTCGACGt                          <  2:1038750/221‑1 (MQ=255)
                                                                                                                                                                                                                      gagCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAg                                                                                                                                                                                  >  2:504503/1‑87 (MQ=255)
                                                                                                                                                                                                                      gagCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAg                                                                                                                                                                                  <  1:504503/87‑1 (MQ=255)
                                                                                                                                                                                                                        gctttttgtgGAAGAAAAAAACTCCACCGCTATGCTGACCACGTTCAACGAAGGCAACATGAAGCCGATTATGGATCTGCTTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCTGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTATCGACGGCGATGACGTGGTTTACCACAACTATTTCGACGt                          <  2:509440/229‑1 (MQ=255)
                                                                                                                                                                                                                             tggtgGAACAGAAAACCCCCACCGGCATGCTGACCAAGTTCAACGAAGTCAACATGAAGCCGATTATGGAACTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTATCGACGGCGATGACGTGGTTTACCACAACTATTTCGACGTCAGCATg                   <  1:799135/236‑1 (MQ=255)
                                                                                                                                                                                                                              gctgGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGCAACGTTACCCGGAAGTGAACGCTTCTATCGACGGCGATGACGGGGTTTCCCCAAACTATTTCACCGTCAgactgc                  >  1:407656/1‑234 (MQ=255)
                                                                                                                                                                                                                                   aaGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCt                                                                                                                                    >  2:1052615/1‑120 (MQ=255)
                                                                                                                                                                                                                                   aaGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCt                                                                                                                                    <  1:1052615/120‑1 (MQ=255)
                                                                                                                                                                                                                                              tCCCCCGCCATGCTGACCCCGTTCAACGAAGTCAACATGAAGCCGATTATGGACCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTATCGACGGCGATGACGTGGTTTACCACAACTATTTCGACGTCAGCATGGCGGTTTCTACGCcgcg  <  1:189349/239‑1 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                              
TGCTGGCTGAACACAATCTCGACGCCAGCGCCATTAAAGGCACCGGTGTGGGTGGTCGTCTGACTCGTGAAGATGTGGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTATCGACGGCGATGACGTGGTTTACCACAACTATTTCGACGTCAGCATGGCGGTTTCTACGCCGCG  >  NC_000913/761883‑762351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: